Method for predicting toxaemias of pregnancy
SUBSTANCE: predicting toxaemia of pregnancy is ensured by determining 24-hour urine placenta-like alkaline phosphatase and lactoferrin when the woman is 20-22 weeks pregnant, and then 2 weeks later, and the measured placenta-like alkaline phosphatase and lactoferrin of more than 16.0 ng/ml enable estimating potential toxaemia of pregnancy.
EFFECT: method enables predicting potential toxaemia of pregnancy, including the comprehensive assessment of protein risk factors taking into account the clinical presentation and significance of each factor in numeric equivalent.
The invention relates to medicine, namely to biochemistry to predict the development of preeclampsia in pregnant women.
Preeclampsia is a syndrome of multiple organ functional failure, which develops as a result of pregnancy or aggravated by the pregnancy. In the classification of preeclampsia include swelling pregnant, proteinuria, hypertension, preeclampsia and eclampsia. In recent years in pregnant women there is a significant increase of OPG-gestosis, the Frequency of preeclampsia according to the median estimates in the literature is 7-22% of all births. Preeclampsia remains a major cause of perinatal morbidity (64-68%) and mortality (18-30%). In the structure of causes of maternal mortality in the Russian Federation preeclampsia steadily ranks third and is of 11.8 to 14.8% (Repin, M. A., 2010. Gridchin A. L., 2012, Ailamazyan, 2013). The urgency of the problem and also due to the severe consequences of this disease. Preeclampsia leads to increased frequency of perinatal complications in mother, fetus and newborn, negatively affects the health of children in later years of life (Sidorova I. S., 2003; Savelyeva G. M., 2010; L. V. Vasilenko et al., 2012). In the pathogenesis of preeclampsia, premature birth pregnancy, the retardation (IUGR) plays a pivotal role dysfunction of the vascular endothelium (Sukhikh G. T. et al., 2008).
It is known that in women with Geus�eskers are observed impairment of many metabolic cycles:
aerobic oxidation in the Krebs cycle, lipid peroxidation, antioxidant protection, endogenous synthesis of prostaglandins, lipid metabolism, immune reactions, all contribute to the dysfunction of the vascular endothelium of the placenta, the change of rheological properties of blood, thrombophilic States. Therefore, the development of an integrated approach to the prediction and prevention of preeclampsia, severe forms of gestosis in pregnant women is an important problem in modern biochemistry and obstetrics.
In the practice of biochemistry known methods of predicting the development of preeclampsia, based on biomicroscopy vessels of the conjunctiva of the eye by slit lamp with computer processing of data by computer programs Adobe Photoshop CS in women in the first trimester of pregnancy. Conducted analysis of chroma pixels of a digital image of blood vessels of the conjunctiva of the eye in the disruption of the microcirculation in comparison with the chromaticity of the pixels in the image of the vessels of the bulbar conjunctiva of healthy subjects to assess the microcirculation was determined in points): score of 7-8 is the norm, aggregation of erythrocytes no; 9-10 points - increased erythrocyte aggregation; 11 points - aggregation of erythrocytes mild degree; 12 points - moderate erythrocyte aggregation; 14 or more points - pronounced aggregation, there is a severe disturbance of processes of microcirculatory�.
Blood test for homocysteine was performed in the first trimester of pregnancy by ELISA. Conditionally normal levels of homocysteine in the blood for women were considered from 4.6 to 12.4 µmol/L. the Levels of homocysteine in the blood in the first trimester of pregnancy proportional to the severity of preeclampsia. With the development of preeclampsia mild severity indicators of homocysteine was 13.4±0.3 μmol/l, with an average severity - 15,9±0.4 µmol/l, with severe degree of 31.4±0.7 µmol/l (p<0,001) (Odnokozova O. C., L. V. Vasilenko, trackin N. And., Kolbenev O. I. pregnancy, childbirth and infant health after the preventive treatment of preeclampsia in pregnant women at risk // Saratov scientific medical journal. - 2009. - T. 5. - No. 4. - Pp. 533-537). However, this predictive model is based on the combined determination of microcirculation and blood levels of homocysteine, has not proven effective for the prediction of preeclampsia as based on factors that are only remotely associated with the pathogenesis of gestosis and placental insufficiency (Roach m, Lu j, Pilepich MV., Asbell SO., Mohiuddin M., Terry R. et al.: Four prognostic groups predict long-term survival from prostate cancer following radiotherapy alone on Radiation Therapy Oncology Group clinical trials. Int. J. Radiat. Oncol. Biol. Phys. 2010, 1; 47:609-615; T. Steuber, M. Graefen, A. Haese et al. Validation of a nomogram for prediction of side-specific extracapsular extension at radical prostatectomy. J. Urol. 2006; 17:939 - 14).
There are also known methods of determination of other biochemical markers such as prostaglandins in combination with means for determining vascular tone. In pregnant women in trimester 1 determine the average MMPI profile, the orientation of vegetative regulation, the type of Central hemodynamics and the level of prostaglandins in serum and increasing the average MMPI profile, the sympathetic orientation of vegetative regulation, hypokinetic type of blood circulation and reduction of prostacyclin by increasing thromboxane predict the occurrence of preeclampsia (stolnikova I. I. Shpak, L. V., " a METHOD of PREDICTING OPG-GESTOSIS IN PREGNANT women WITH ESSENTIAL HYPERTENSION).
All the described methods have drawbacks:
- the above methods are based on a combination of sophisticated instrumental methods of examination (biomicroscopy retinal vessels, functional study of the Central nervous system and vascular tone), psychological tests (score on a scale of MMPI) and complex biochemical analyses determination of prostaglandins.
- lack of correlation of the level of prostaglandins with the MMPI profile;
- the technical complexity of implementation;
- the need for blood tests in the first 12 hours of collection because of the instability of prostaglandins.
- low accuracy of the research.�tion and the lack of clear limits for assessing the prognosis of developing preeclampsia.
Closest to the proposed method is a test to determine serum and urine other markers of preeclampsia Inhibin A and Activin a level in peripheral blood and urine correlates with the likelihood of developing preeclampsia. Especially pronounced is the increase in the concentration of the proposed markers in the urine (more than 100 times). The authors propose to consider the levels of inhibin A and Activin a in the blood equal or above 5.0 ng/ml, and the levels of inhibin A and Activin A in the urine above 1.0 PG/ml, as the boundary of the assessment of the probability of developing preeclampsia. (Muttukrishna S., Hyett J., Paine M., Moodley J., Groome N. and Rodeck C. Can vein and maternal urinary levels of activin A and inhibin A in pre-eclampsia patients Clinical Endocrinology - 2006 - v. 64 - p. 469-473).
However, the disadvantages of this method are:
- fuzzy differentiation in the evaluation of prognosis of preeclampsia;
- no communication with clinical signs of preeclampsia, in addition to hypertension;
- narrow range of concentrations of inhibin A and Activin A, proposed by the authors to assess the development of preeclampsia;
- complicated calculation for standardization and accounting of urine;
- the lack of data on the prediction of preeclampsia (preeclampsia) in the 1st or early 2nd trimester of pregnancy.
The invention is directed to predicting the development of preeclampsia on the basis of non-invasive dynamic immunochemical determination of placental alkaline phosphatase and lactoferrin in daily urine baie�variable item, correlated with subsequent development of preeclampsia. Said technical result is achieved in that to predict the development of preeclampsia, determine the concentration of placental alkaline phosphatase and lactoferrin, characterized in that determine the concentration of placental alkaline phosphatase and lactoferrin in daily urine of pregnant women at 20-22 weeks of pregnancy and again after 2 weeks and on the sums of the concentrations of placental alkaline phosphatase and lactoferrin concentrations above 16.0 ng/ml predict the development of preeclampsia.
The proposed method was successfully tested on the 96 women in the period from 2009 to 2012, observed in the antenatal clinic OKB №1 Astrakhan from 15 to 36 weeks of pregnancy, with an observation interval of 2 weeks.
The method is illustrated by the following clinical examples of its implementation.
Clinical example 1
Patient A., 23 years old (case History No. 14329). Turned 21.02.2009 G. Daily urine on the study of markers taken on 20 week of pregnancy 22.02.2009 G. concentrations of placental alkaline phosphatase was 0.9 ng/ml lactoferrin was 6.6 ng/ml, which amounted to a total of 7.5 ng/ml At 22 weeks of pregnancy (06.03.2009) urine for the study of markers taken again. Concentrations of placental alkaline phosphatase was 1.8 ng/ml, lactoferrin, and 11.1 ng/ml, for a total of two studies �left of 20.4 ng/ml. Prognosis - the likelihood of developing preeclampsia.
With 28 weeks of the current pregnancy was with transient proteinuria, recurrent swelling of the lower extremities. Given birth to the term, vaginally a living male fetus, weighing 3250 g with estimation on Apgar scale - 6-7 points.
Clinical example 2
Patient S., 24 years old (case History No. 23124). Turned 01.11.2010 Daily urine on the study of markers taken on 21 week of pregnancy (02.11.2010). Concentrations of placental alkaline phosphatase was 0.1 ng/ml lactoferrin 2.5 ng/ml, which was 2.6 ng/ml. 16.11.2010 urine in a study of markers taken again at 23 weeks of pregnancy. Concentrations of placental alkaline phosphatase was 4.7 ng/ml lactoferrin to 4.3 ng/ml, for a total of two studies was 11.6 ng/ml the Prognosis - the likelihood of developing preeclampsia is missing. During pregnancy carried out correction HELL, it was recommended that a diet low in salt. The pregnancy was uneventful. Given birth to the term, vaginally full-term baby girl weighing 3100 g with estimation on Apgar scale - 7-8 points.
Clinical example 3
Patient Sh, 26 years old (case History No. 10217), entered 02.04.2011 G. Daily urine on the study of markers taken at 22 weeks of pregnancy (03.04.2010). The values of concentration�ation placental alkaline phosphatase was 10.1 ng/ml, lactoferrin is 14.5 ng/ml, which in total amounted to 24.6 ng/ml. 16.04.2010 urine in a study of markers taken again on 24 week of pregnancy, the concentrations of placental alkaline phosphatase amounted to 13.0 ng/ml lactoferrin of 10.3 ng/ml, for a total of two studies was 47.8 ng/ml. Prognosis - the likelihood of developing preeclampsia. The pregnancy proceeded with transient proteinuria, recurrent swelling of the lower extremities. With 30 weeks of pregnancy was complicated by severe late preeclampsia to eclampsia. Given birth in the period 39-40 weeks of gestation by cesarean section live male fetus, weighing 2950 g with estimation on Apgar scale - 6-7 points.
The basis of the invention was based on the results of a survey of 96 pregnant women. The average age of patients was 23.5±0.4 years (21 to 24 years) (tab. 1).
The diagnosis of preeclampsia was established on the basis of clinical (complaints, the presence of hidden and overt edema, hypertension, the presence of concomitant extragenital pathology, the degree of violation of the utero-placental and fetal-placental blood flow, IUGR of the fetus, signs of fetal hypoxia), laboratory data (OAK, OAM, blood chemistry, coagulation, urinalysis in General, the Reichstag, Nechiporenko. The study of daily proteinuria), data instrumental examination (ultrasound, doplerometriya, CTG, studied�e fundus, monitoring blood pressure) were assessed severity of preeclampsia on the scale of G. M. Savelieva.
The conducted research has allowed to produce a comprehensive assessment of risk factors with regard to informative value of each factor. The results of the study are presented in table 1. Positive = 12; false positive = 8; false negative = 4. Sensitivity: True Positive/True Positive + false negative = 75,0%.
Specificity: True negative/true negative + false positive = 89.7 per cent.
Thus, this means: 75,0% of pregnant women with a total level PDF and lactoferrin above 16.0 ng/ml predict the development of preeclampsia appears to be true. Specificity is equal to 89.7% of it, therefore, in 89.7% of patients with a known negative Outlook, the test results are negative. The negative difference 14.7% between significantly positive and significantly negative Outlook is the uncertainty factor and in this case opens the possibility for the introduction of new prognostic markers to improve sensitivity.
The proposed method has the following advantages:
- improving the accuracy of predicting the development of preeclampsia on the basis of determination of total concentrations of placental alkaline phosphatase and lactoferrin with high verootstupnichestva 75,0% (r=0,7; P < 0,00001).
- has the availability and economic feasibility, as it is based on an assessment of only put into wide practice of laboratory methods and does not require expensive laboratory equipment.
- has the simplicity as it is based on non-invasive study
- is open prognostic system, since it allows to easily introduce additional laboratory markers to improve sensitivity and reduce the number of false positive results, i.e. to improve the specificity.
A METHOD of PREDICTING PREECLAMPSIA IN PREGNANT women
A method of predicting gestosis of pregnant women, namely, in the biochemical study of biological fluids, characterized in that determine the concentration of placental alkaline phosphatase and lactoferrin in daily urine of pregnant women at 20-22 weeks of pregnancy and again after 2 weeks and on the sums of the concentrations of placental alkaline phosphatase and lactoferrin concentrations above 16.0 ng/ml predict the development of preeclampsia.
SUBSTANCE: invention relates to field of medicine. Claimed is method, including determination of presence or absence of K-rasG12D mutation. Presence of K-rasG12D mutation points to the fact that patient will not respond to treatment with B-Raf inhibitor.
EFFECT: invention provides effective method of identifying patient who does not respond to treatment with B-Raf inhibitor.
6 cl, 34 dwg, 12 tbl, 3 ex
SUBSTANCE: invention refers to medicine, particularly to oncology, and can be used for detecting endogenous intoxication (EI) symptoms in the patients suffering colorectal cancer. That is ensured by studying the complete blood count and deriving an integral intoxication index (III) by formula:
EFFECT: invention provides diagnosing the absence (III=4,6) or presence (III=10,4) of the EI symptoms and associated respiratory disorders and enables assessing the therapy efficacy.
SUBSTANCE: predicting heart rhythm disorder in the patients with pre-excitation syndromes is ensured by measuring patient's blood plasma matrix metalloproteinase-9 (MMP-9), and if the measured value is less than 90.6 ng/ml, the favourable clinical course accompanied by no heart rhythm disorders (HRD) is predicted, whereas the MMP-9 concentration of more than 90.6 ng/ml enables predicting HRD.
EFFECT: method enables predicting HRD in the patients with pre-excitation syndromes by high sensitivity and specificity of the method with its low invasiveness and no counterindications.
SUBSTANCE: technique consists in measuring a pinopod count in the germinal epithelium, an expression level of progesterone and oestrogen alpha receptors in the endometrial stroma, calculating their ratio, and determining an expression level of implantation LIF factor on the 21st-24th day of the menstrual cycle ('implantation window'). If the pinopod count is less than 35%, the expression ratio of progesterone and oestrogen alpha receptors in the endometrial stroma less than 3.5, as well as the LIF expression level of less than 2 points, disturbed fertility associated with uterine myoma is diagnosed.
EFFECT: technique enables applying the differentiated approach to managing the patients planning pregnancy, suffering uterine myoma undeforming the uterine cavity, as well as with myomatous nodule up to 5,0 cm in diameter.
2 ex, 4 dwg
SUBSTANCE: method involves sampling a biological tissue, introducing a biologically active substance therein, keeping the prepared mixture in a thermostat at 37°C and incubating, performing microscopic examination for identifying the forms of trichomonads. The sampled biological tissue represents the urogenital mucosa that is incubated for 24 h in a nutrient medium. The prepared sediment is separated and added with the biologically active substance nitrosoguanidine in an amount of 500 mcg/ml. That is followed by incubating for 15 min at +37°C; a phosphate buffer with pH 5.6 heated to +37°C is added and centrifuged twice at 1000 rpm for 10 min. The material is placed into the nutrient medium for trichomonad growing, incubated for at least 24 hours, and microscopic examination is used to identify atypical nonflagellate and typical round and amoeboid forms of trichomonads.
EFFECT: using the declared invention enables the effective, fast and reliable identification of typical changed and atypical forms of trichomonads.
3 dwg, 3 ex
SUBSTANCE: method involves measuring pregnant women's blood anti-cytomegalovirus antibody titre and glutathione reductase activity in erythrocytes of the obstetric patient suffering the cytomegaloviral infection. If the anti-cytomegalovirus antibody titre is 1:1600, whereas the measured glutathione reductase activity is below 4.48±0.22 units/gHb, the aggravated cytomegaloviral infection is diagnosed.
EFFECT: higher diagnostic technique.
SUBSTANCE: group of inventions relates to medicine, cosmetology, production of food products, vitamins, food supplements, drugs and describes versions of device for realisation of non-invasive potentiometric determination of oxidant/antioxidant activity of biological tissues, which includes device for measuring potentials and double-sided electrode, made in form of plate with similar working surface, covered with electricity-conducting gel, containing mediator system. Electrodes are fixed on biological tissue in such a way that one working surface, playing role of measuring electrode, is in direct contact with biological tissue via gel, second working surface pale role of comparison electrode. Electrodes contact with each other via gel, with oxidant/antioxidant activity being determined by formulae with application of difference between final and initial potentials.
EFFECT: simplification, as well as increase of accuracy and reliability of determination, is achieved.
14 cl, 3 tbl, 4 dwg
SUBSTANCE: invention represents a method for the prediction of preeclampsia in the second trimester of pregnancy by blood examination, differing by the fact that the activity of acid and neutral proteinases is measured in blood serum of the women 7-8 weeks pregnant; if the activity of acid proteinases is more than 5.6 mcmole/l, while the activity of neutral proteinases is more than 3.9 mcmole/l, the preeclampsia progression in the second trimester of pregnancy is predicted.
EFFECT: higher accuracy and specificity of the method for the prediction of gestational toxicosis.
SUBSTANCE: invention relates to medicine, namely to a method of automated morphometric myelofibrosis diagnostics. The method essence consists in the fact that overview images of zones with different optical properties with determinable fibrous and heamopoietic properties of a biological tissue are performed. Ratios of areas of the said zones of at least three paraffin cuts of trepanobiopsy samples are calculated. The coefficient (Cop) is calculated as the ratio of the fibrous tissue area to the area of the heamopoietic tissue by formula. If the value Cop ≥14.5%, myelofibrosis is diagnosed.
EFFECT: application of the claimed method makes it possible to increase the accuracy and improve the efficiency of myelofibrosis diagnostics.
7 cl, 1 tbl, 4 dwg, 1 ex
SUBSTANCE: testicular germ cells are measured quantitatively. That is ensured by 50-day oral administration of selexen and ascorbic acid into male white rats in doses 1.5 and 500 mg/kg of animal's body weight respectively once a day. 14 days later, administering the selenium-containing biocomplex is accompanied by the 30-minute daily exposure to microwave radiation at 42 GHz (λ=7.1 mm) for 30 days. Once the experimental exposures are completed, the corrective properties of the biocomplex as having an effect on the morphofunctional state of epididymal sperm cells are assessing by formula: MFSI=A+B, wherein MFSI is a morphofunctional state index, A is a portion of normal sperm cells in relation to the reference, and B is a portion of moving sperm cells in relation to the reference. If the MFSI value is 1.3 or more, the spermatogenesis correction is considered to be ineffective, while the MFSI value being more than 1.3 shows the effective spermatogenesis correction if exposed to microwave radiation.
EFFECT: invention enables assessing the spermatogenesis correction efficacy with underlying administration of the biocorrector.
2 tbl, 3 dwg, 3 ex
FIELD: medicine, psychiatry.
SUBSTANCE: one should isolate DNA out of lymphocytes of peripheral venous blood, then due to the method of polymerase chain reaction of DNA synthesis one should amplify the fragments of hSERT locus of serotonin carrier gene and at detecting genotype 12/10 one should predict the risk for the development of hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis.
EFFECT: more objective prediction of disease development.
FIELD: medicine, urology.
SUBSTANCE: one should conduct subcutaneous prevocational tuberculin test and, additionally, both before the test and 48 h later it is necessary to perform the mapping of prostatic vessels and at decreased values of hemodynamics one should diagnose tuberculosis. The information obtained should be documented due to printing dopplerograms.
EFFECT: more reliable and objective information.
1 ex, 1 tbl
FIELD: molecular biology.
SUBSTANCE: the suggested innovation deals with the fact that nucleic acids should be isolated directly out of the sample without pipetting stage but with the help of interconnected reservoirs being prepared beforehand. The above-mentioned vessels should be applied either separately or being interconnected according to standard microtitrating format. The sample should be mixed with a lyzing buffer and nucleic acids are bound with matrix in closed system including, at least, two interconnected reservoirs. Forced movement of sample's mixture and buffer back and forth from one reservoir into another one for several times through narrow passage provides their thorough intermixing. The method provides quick and safe isolation of nucleic acids.
EFFECT: higher efficiency.
44 cl, 4 dwg, 1 ex
FIELD: medicine, phthisiology, microbiology.
SUBSTANCE: diagnostic material is poured preliminary with chlorohexidine bigluconium solution, homogenized, kept at room temperature for 10-12 h and centrifuged. Precipitate is poured with Shkolnikova's liquid medium, incubated at 37oC for 3 days, supernatant part of Shkolnokova's medium is removed, fresh Shkolnikova's medium is added, and precipitate is stirred and inoculated on the dense cellular egg media. Sensitivity of the strain is determined in 3 weeks by the presence of growth in the control tube only. Invention provides enhancing precision and reducing time for assay. Invention can be used in assay for medicinal sensitivity of tuberculosis mycobacterium.
EFFECT: improved assay method.
FIELD: medicine, biotechnology, pharmacy.
SUBSTANCE: invention relates to agents used for treatment of pathological states associated with disorder of synthesis of neuromediating substances. Method involves the development of pharmaceutical composition and a method for it preparing. Pharmaceutical composition represents subcellular synaptosomal fractions: synaptic membranes, "light" synaptosomes and "heavy" synaptosomes prepared from gray matter of cerebral hemispheres from experimental animals based on the goal-seeking modification of humoral mediators of nerve endings transformed to synaptosomes in development and regression of malignant processes. The composition provides inhibiting the growth of tumor cells, to elevate span-life of patients with ascite Ehrlich's sarcoma, breast adenocarcinoma Ca-755, Wolker's carcinosarcoma-256.
EFFECT: valuable medicinal and anti-tumor properties of composition.
12 cl, 3 tbl, 3 ex
SUBSTANCE: method involves carrying out microscopic examination of blood serum samples taken from femoral vein and cubital vein. Femoral vein sample is taken on injured side. The examination is carried out before and after treatment. The blood serum samples are placed on fat-free glass slide in the amount of 0.01-0.02 ml as drops, dried at 18-30°C for 18-24 h. The set of pathological symptoms becoming larger or not changed after the treatment in comparison to sample taken before treatment, and morphological picture of samples under comparison taken from the cubital vein showing no changes or being changed to worse, the treatment is considered to be effective.
EFFECT: enabled medicamentous treatment evaluation in course of treatment to allow the treatment mode to be changed in due time; avoided surgical intervention (amputation); retained active life-style of aged patients.
FIELD: medicine, clinical toxicology.
SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
EFFECT: higher accuracy of prediction.
2 ex, 3 tbl
FIELD: medicine, juvenile clinical nephrology.
SUBSTANCE: disease duration in case of obstructive pyelonephritis should be detected by two ways: either by detecting the value of NADPH-diaphorase activity, as the marker of nitroxide synthase activity in different renal department and comparing it to established norm, or by detecting clinico-laboratory values, such as: hemoglobin, leukocytes, eosinophils, urea, beta-lipoproteides, lymphocytes, neutrophils, the level of glomerular filtration, that of canalicular reabsorption, urinary specific weight, daily excretion of oxalates, arterial pressure, and estimating their deviation against average statistical values by taking into account a child's age.
EFFECT: higher efficiency of detection.
7 dwg, 1 ex, 6 tbl
FIELD: medicine, urology.
SUBSTANCE: the present innovation deals with differential diagnostics of prostatic cancer and other prostatic diseases at the stage of primary inspection. The method includes the detection of PCA and calculation of probability coefficient for prostatic cancer (PCC) by the following formula: where e - the foundation of natural logarithm (e=2.718…), PCA - the level of total blood PCA in ng/ml, V - patient's age in years. At PCC value being above 0.2 one should diagnose prostatic cancer and to establish final diagnosis one should perform polyfocal prostatic biopsy. The method enables to increase accuracy of diagnostics at decreased number of unjustified prostatic biopsies.
EFFECT: higher efficiency of diagnostics.
FIELD: medicine, biology.
SUBSTANCE: invention relates to nutrient medium used for accumulation of cells for the following cytological and/or immunocytochemical analysis carrying out. Invention relates to medium containing salts NaCl, KCl, anhydrous CaCl2, MgSO4 x 6 H2O, MgCl2 x 6 H2O, Na2HPO4 x 2 H2O, KHPO4, NaHCO3, and also glucose and Henx's solution, 10% albumin solution and polyglucin taken in the ratio 1:1:1. Invention provides enhancing the preservation of cells.
EFFECT: improved an valuable properties of nutrient medium.