Method of obtaining encapsulated native blood, possessing supramolecular properties

FIELD: medicine.

SUBSTANCE: method of obtaining encapsulated native blood, which is characterised by the fact that suspension of native blood in diethyl ether is dispersed in mixture of sodium carboxymethylcellulose in benzene, in presence E472, mixed, with pouring hexane and water to obtained mixture, obtained suspension is filtered and dried under specified conditions.

EFFECT: method makes it possible to simplify and accelerate process of obtaining encapsulated native blood.

2 dwg, 2 ex

 

The invention relates to the field of encapsulation.

Previously known methods for producing microcapsules.

In us Pat. 2173140, IPC A61K 009/50, A61K 009/127, Russian Federation, published 10.09.2001, a method of producing kremnijorganicheskih microcapsules using a rotary cavitation equipment with high shear forces and powerful hydroacoustic phenomena sonic and ultrasonic range for dispersion.

The disadvantage of this method is the use of special equipment - rotary-cavitational installation, which has the ultrasonic effect, which influences the formation of the microcapsules and thus may cause adverse reactions due to the fact that ultrasound has a destructive effect on polymers of protein nature, therefore, the proposed method is applicable when working with polymers of synthetic origin.

In us Pat. 2359662, IPC A61K 009/56, A61J 003/07, B01J 013/02, A23L 001/00 published 27.06.2009, Russian Federation, proposed a method of obtaining microcapsules of sodium chloride with the use of spray cooling in the spray tower Niro under the following conditions: air temperature at the inlet 10°C, the temperature at the outlet 28°C, the speed of rotation of the spray drum 10000 rpm/min Microcapsules according to the invention have improved stability and provide�ivut adjustable and/or prolonged release of the active ingredient.

The disadvantages of the proposed method are the duration of the process and the use of special equipment, certain set of conditions (the air temperature at the inlet 10°C, the temperature at the outlet 28°C, the speed of rotation of the spray drum 10000 rpm/min).

The closest method is the method proposed in us Pat. 2134967, IPC A01N 53/00, A01N 25/28, published 27.08.1999, Russian Federation (1999). In water dispersed solution of a mixture of natural lipids and PYRETHROID insecticide in a weight ratio of 2-4:1 in an organic solvent, which leads to simplification of the method of microencapsulation.

The disadvantage is the dispersion in an aqueous medium, which makes the proposed method applicable for obtaining microcapsules of water-soluble drugs in water-soluble polymers.

The technical objective is the simplification and acceleration of the process of production of microcapsules, reducing loss upon receipt of the microcapsules (increase of the yield by weight).

The solution of the technical problem is achieved by a method of producing encapsulated native blood, characterized in that as the shell of the microcapsules used sodium carboxymethylcellulose, and as the kernel - native blood when receiving the encapsulated particles by deposition of nerastvorim with the use of hexane as the precipitant, the process received�of the microcapsules is carried out without special equipment.

A distinctive feature of the proposed method is the production of microcapsules by the method of deposition by nerastvorim using hexane as the precipitant, and the use of sodium carboxymethylcellulose as the shell particles and native blood as the kernel.

The result of the proposed method are obtaining microcapsules of native blood in the shell - sodium carboxymethylcellulose.

Fig.1 - self-organization nanocapsules native blood in sodium carboxymethylcellulose in a ratio of sheath: core 3:1 at a concentration of 0.25%: a) with the increase in 505 times, b) with the increase of 620 times) with increasing 930 times, g) with magnification of 1200 times, d) with increasing 1770 times, (e) with increasing 2830 times.

Fig.2 - self-organization nanocapsules native blood in sodium carboxymethylcellulose in a ratio of sheath: core 3:1 at a concentration of 0.125%: a) with the increase in 1770 times, b) with increasing 2830 times.

EXAMPLE 1. Obtaining microcapsules of native blood in sodium carboxymethylcellulose

A suspension of 100 mg of native blood in 1 ml of diethyl ether is dispersed in a solution of sodium carboxymethyl cellulose in benzene containing the specified 300 mg of the polymer in the presence of 0.01 g of the drug E472 (an ester of glycerol with one or two molecules of dietary fatty acids and one or two molecules of citric acid, and limo�Naya tribasic acid as can be etherification other glycerides and as oxanilate - other fatty acids. Free acid groups can be neutralized with sodium with stirring 1300 Rev/sec. Next, pour 5 ml of hexane and 1 ml of water. The resulting suspension was filtered and dried at room temperature.

Received 0,396 g of powder of microcapsules. The yield was 99%.

EXAMPLE 2. Investigation of self-microcapsules from solutions

From the powder of the microcapsules obtained by the method described in example 1, there were prepared aqueous solutions of concentrations 1%, 0,5%, 0,25%, 0,125% etc. by dilution of the solution twice. A drop of each of the prepared solutions were placed on a glass slide prior to complete drying, and dried on the surface was carried out confocal scanning microscopy.

Thus, the obtained microcapsules native blood with a high yield without special equipment within 10 min. the Formation of microcapsules occurs spontaneously through non-covalent vzaimodeistvie, and this suggests that they are characterized by self-Assembly. Shown in Fig.1, 2 patterns are ordered, so they are self-organizing. Consequently, the encapsulated native blood has its supramolecular properties.

A method of producing encapsulated native blood, characterized by the fact that a suspension of 100 mg of native blood in 1 ml of diethyl ETH�and dispersed in 300 mg of sodium carboxymethyl cellulose in benzene, in the presence of 0.01 g E, was stirred at 1300 Rev/sec, then to this mixture was poured 5 ml of hexane and 1 ml of water, the resulting suspension was filtered and dried at room temperature.



 

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