Diagnostic technique for aggravated cytomegaloviral infection in third trimester of gestation accompanied by erythrocyte glutathione reductase reduction
SUBSTANCE: method involves measuring pregnant women's blood anti-cytomegalovirus antibody titre and glutathione reductase activity in erythrocytes of the obstetric patient suffering the cytomegaloviral infection. If the anti-cytomegalovirus antibody titre is 1:1600, whereas the measured glutathione reductase activity is below 4.48±0.22 units/gHb, the aggravated cytomegaloviral infection is diagnosed.
EFFECT: higher diagnostic technique.
1. The purpose of the invention is to identify that by reducing the activity in erythrocytes of peripheral blood of pregnant women in the third trimester of gestation glutationreductaza marked exacerbation of cytomegalovirus infection.
2. The invention relates to medicine, namely to develop a method for the diagnosis of acute cytomegalovirus infection in the decrease in peripheral blood erythrocytes glutationreductaza.
3. Disclosure of the invention
Along with glycolysis (anaerobic breakdown of glucose) in erythrocytes occurs, the direct oxidation of glucose to carbon dioxide and water - ecomonically shunt . In this cycle NADP restored in NADP-N. Violation of the reactions of pentose cycle in certain diseases is associated with the destruction of the structures of red blood cells and the onset of hemolytic crises . Formed in the reaction of pentose cycle NADP-H is used in red blood cells to restore oxidized glutathione with the participation glutationreductaza. Restoration of glutathione with the participation of NADP-N glutationreductaza associated with the regeneration of the oxidized form of NADP-N. Restored glutathione (GSH) is 96% participates in the reaction with thiol groups. Maintaining glutathione in the reduced state it is necessary for protection of erythrocyte enzymes from inactivation and prevention of the action of peroxides on meme�the wound of red blood cells [4-6]. When oxidation of the reduced glutathione decreases the intensity of glycolysis in erythrocytes . Given the above, to maintain normal level glutationreductaza in erythrocytes provides sustainable fermentative metabolism and morphological and functional integrity of membranes. When acute infectious processes in the body, pregnant women marked decrease in the activity glutationreductaza in peripheral blood erythrocytes . Changing the content glutationreductaza during exacerbation of cytomegalovirus infection in the available literature were not observed.
4. New technical problem
Determining the level of glutationreductaza in peripheral blood erythrocytes of pregnant women in the third trimester of gestation, diagnosed exacerbation of cytomegalovirus infection.
5. In the available literature counterparts on this issue is not marked.
6. The implementation of the invention
6.1. The research was performed in the obstetric Department of the research center of the far Eastern scientific center of physiology and pathology of respiration SB RAMS. The study included 25 pregnant women in the age group of 18-25 years with chronic cytomegalovirus infection in the acute stage (study group) and 20 pregnant women without pathology (control group). The diagnosis of exacerbation of chronic cytomegalovirus infection was established with a comprehensive study of peri�aricescu blood for the presence of IgM or a fourfold or more increase in titer of IgG antibodies in paired sera in the dynamics after 10 days, the avidity index (65%), and the availability of DNA to CMV.
6.2. The research was carried out taking into account requirements of the Helsinki Declaration of the world medical Association on Ethical principles for conducting research with people" as amended in 2008 and the good clinical practice in the Russian Federation, the approved order of the Ministry of the Russian Federation No. 266 dated 19.06.2003.
6.3. Verification of CMV, the definition of type-specific antibodies, the avidity index was performed by ELISA on spectrophotometer "Stat Fax 2100" (USA) using test systems CJSC "Vector-best" (Novosibirsk), using PCR apparatus DT-96 (Russia) using the kits of the NGO "DNA-technology" (Moscow).
6.4. Glutationreductaza was determined by a spectrophotometric method using a set of reagents "Sentinel Diagnostics (Italy) on spectrophotometer "Stat Fax-2100" (USA).
6.5. Statistical analysis was performed parametric unpaired student's criterion and the method of Fisher.
In the process of research the following results were obtained: in the peripheral blood erythrocytes of pregnant women in the third trimester of gestation reduced content glutationreductaza to 4,48±0,22 U/b (control - 9,00±0,15 U/b). Examination of peripheral blood notes that at this time there is an exacerbation of cytomegalovirus infection until the titer of antibodies to cytomegalovirus�su 1:1600.
1. Lutsenko M. T., Andrievskaya I. A. "a Method for diagnosing respiratory function of erythrocytes of pregnant women by determining the activity glutationreductaza in peripheral red blood cells in cytomegalovirus infection." Patent No. 2402276 published 27.10.2010 Bulletin "Inventions, utility models," No. 30.
2. Ryabov S. I. fundamentals of physiology and pathology of erythropoiesis. L.: Medicine. Leningrad branch, 1971. 251 S.
3. Fedorov N. And. Normal hematopoiesis and its regulation. M.: Medicine, 1976. 543 S.
4. Brewer G., Pewell R. D. Hexokinase activity as a function of age of the human erythrocytes / / Nature (Lond). 1963. Vol. 199. 704 p.
5. Dische Z. The pentose phosphate metabolism in red cells // In: The red blood cell, Ed. Bishop Ch. and Surgenor D. New York, 1964. 189 p.
6. Jaffe E. R. Introduction to discussion of red cell. GSH metabolism the function of reduced glutatione in human erythrocytes // "Exp. Eye Res". 1971. Vol. 11. 306 R.
Method for the diagnosis of acute cytomegalovirus infection in the third trimester of gestation, which includes the determination in the blood of pregnant titer of antibodies to cytomegalovirus and the activity of glutathione reductase in erythrocytes of mothers with cytomegalovirus infection, and if the value of the titer of antibodies to cytomegalovirus 1:1600 and significance of glutathione reductase activity below 4,48±0,22 U/b diagnosed exacerbation of cytomegalovirus infection.
SUBSTANCE: group of inventions relates to medicine, cosmetology, production of food products, vitamins, food supplements, drugs and describes versions of device for realisation of non-invasive potentiometric determination of oxidant/antioxidant activity of biological tissues, which includes device for measuring potentials and double-sided electrode, made in form of plate with similar working surface, covered with electricity-conducting gel, containing mediator system. Electrodes are fixed on biological tissue in such a way that one working surface, playing role of measuring electrode, is in direct contact with biological tissue via gel, second working surface pale role of comparison electrode. Electrodes contact with each other via gel, with oxidant/antioxidant activity being determined by formulae with application of difference between final and initial potentials.
EFFECT: simplification, as well as increase of accuracy and reliability of determination, is achieved.
14 cl, 3 tbl, 4 dwg
SUBSTANCE: invention represents a method for the prediction of preeclampsia in the second trimester of pregnancy by blood examination, differing by the fact that the activity of acid and neutral proteinases is measured in blood serum of the women 7-8 weeks pregnant; if the activity of acid proteinases is more than 5.6 mcmole/l, while the activity of neutral proteinases is more than 3.9 mcmole/l, the preeclampsia progression in the second trimester of pregnancy is predicted.
EFFECT: higher accuracy and specificity of the method for the prediction of gestational toxicosis.
SUBSTANCE: invention relates to medicine, namely to a method of automated morphometric myelofibrosis diagnostics. The method essence consists in the fact that overview images of zones with different optical properties with determinable fibrous and heamopoietic properties of a biological tissue are performed. Ratios of areas of the said zones of at least three paraffin cuts of trepanobiopsy samples are calculated. The coefficient (Cop) is calculated as the ratio of the fibrous tissue area to the area of the heamopoietic tissue by formula. If the value Cop ≥14.5%, myelofibrosis is diagnosed.
EFFECT: application of the claimed method makes it possible to increase the accuracy and improve the efficiency of myelofibrosis diagnostics.
7 cl, 1 tbl, 4 dwg, 1 ex
SUBSTANCE: testicular germ cells are measured quantitatively. That is ensured by 50-day oral administration of selexen and ascorbic acid into male white rats in doses 1.5 and 500 mg/kg of animal's body weight respectively once a day. 14 days later, administering the selenium-containing biocomplex is accompanied by the 30-minute daily exposure to microwave radiation at 42 GHz (λ=7.1 mm) for 30 days. Once the experimental exposures are completed, the corrective properties of the biocomplex as having an effect on the morphofunctional state of epididymal sperm cells are assessing by formula: MFSI=A+B, wherein MFSI is a morphofunctional state index, A is a portion of normal sperm cells in relation to the reference, and B is a portion of moving sperm cells in relation to the reference. If the MFSI value is 1.3 or more, the spermatogenesis correction is considered to be ineffective, while the MFSI value being more than 1.3 shows the effective spermatogenesis correction if exposed to microwave radiation.
EFFECT: invention enables assessing the spermatogenesis correction efficacy with underlying administration of the biocorrector.
2 tbl, 3 dwg, 3 ex
SUBSTANCE: invention relates to field of medicine, in particular hepatology. Method of quantitative estimation of histological activity in liver biopsy materials in case of chronic diffuse liver diseases results in protocol of counting in absolute numbers, percent and points of pathological signs, identified in the course of morphological analysis of liver biopsy materials from patients with chronic diffuse liver diseases, by sections: necrosis of hepatocytes, globular and atomised fatty hydropic degeneration of hepatocytes, small cell dysplasia of hepatocytes, portal, periportal, intralobular and central infiltration. After that conversion of absolute values into point system is performed by means of said protocol, and histological activity is determined by number of points.
EFFECT: method makes it possible to create quantitative unification of obtained results in case of said diseases.
FIELD: veterinary science.
SUBSTANCE: method includes detection of leukocytes, protein, haemoglobin in faeces, and PH-reaction of faeces. A sample of faeces is dissolved in distilled water and applied drop by drop onto appropriate test fields of a test strip designed for urine testing. By variation of colour within 1 minute the reaction is assumed as positive. If pH is less than 7.0 or more than 7.5, and faeces contain soluble protein, haemoglobin and leukocytes at the same time, availability of the inflammatory process in the intestines is confirmed.
EFFECT: invention makes it possible to quickly and accurately diagnose inflammatory process in intestines.
2 cl, 2 ex
SUBSTANCE: invention relates to medicine and is intended for the treatment of patients with the perforation of gastric and duodenal ulcer. A laparotomy is performed, operative aid concerning the ulcer perforation is realised, during the operation an exudate is sampled from the abdominal cavity, pH of the exudate is determined. If Ph of the said material is 6.6 and lower, a conclusion about the presence in it of anaerobic flora is made and serves the basis for the administration of an antibacterial preparation, influencing the anaerobic flora, into the scheme of initial antibacterial therapy.
EFFECT: method makes it possible to administer specific antibacterial therapy without waiting for culture results.
SUBSTANCE: method involves the integrated clinical-laboratory and microbiological studies of patient's urogenital discharge to determine an agent's cytomorphotype as shown by culture analysis and evaluating a degree of manifestation of the patient's urogenital inflammation or leucocytosis.
EFFECT: invention enables improving the quality of laboratory diagnostics, provides establishing the accurate diagnosis that improves the clinical effectiveness, and reduces the rate of complication accompanying chronic trychomonad infection.
1 tbl, 2 ex
SUBSTANCE: invention refers to medicine, namely to a method for producing cells and non-cell elements of an atherosclerotic plaque. The substance of the method consists in the fact that a balloon angioplasty is followed by washing the cells and non-cell elements; the produced suspension of the cells and non-cell elements is centrifuged; a supernatant fluid is removed; the cells and non-cell elements are re-suspended; the suspensions of the cells and non-cell elements are placed into charged cytobuckets, which are centrifuged; the produced preparations are used for further analysis.
EFFECT: using the declared method provides producing the patient's cells and non-cell elements of the atherosclerotic plaque after the balloon angioplasty for the main indication.
5 cl, 10 dwg, 2 ex
SUBSTANCE: invention represents a method for predicting thromboembolic complications accompanying surgical management in the patients suffering oncologic pulmonary diseases; the method is characterised by the fact that performing a pulmonary surgery involves ligaturing vessels of an involved lung is preceded by approaching its afferent and efferent vessels followed by blood sampling from each vessel and venous blood sampling from a peripheral vein; after the surgery, free heparin is measured in each sample. Thereafter, the parameter K is calculated as the arithmetic mean free heparin content in the afferent vessel of the involved lung and in the venous blood from the peripheral vein; the calculated parameter K is compared to the quantitative free heparin content P; if K is less than P by 25% or less, a high risk of the postoperative thromboembolic complications is predicted, whereas K being less than P by more than 25%, a low risk of the postoperative thromboembolic complications is predicted.
EFFECT: higher accuracy and objectivity of the early postoperative results.
FIELD: medicine, psychiatry.
SUBSTANCE: one should isolate DNA out of lymphocytes of peripheral venous blood, then due to the method of polymerase chain reaction of DNA synthesis one should amplify the fragments of hSERT locus of serotonin carrier gene and at detecting genotype 12/10 one should predict the risk for the development of hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis.
EFFECT: more objective prediction of disease development.
FIELD: medicine, urology.
SUBSTANCE: one should conduct subcutaneous prevocational tuberculin test and, additionally, both before the test and 48 h later it is necessary to perform the mapping of prostatic vessels and at decreased values of hemodynamics one should diagnose tuberculosis. The information obtained should be documented due to printing dopplerograms.
EFFECT: more reliable and objective information.
1 ex, 1 tbl
FIELD: molecular biology.
SUBSTANCE: the suggested innovation deals with the fact that nucleic acids should be isolated directly out of the sample without pipetting stage but with the help of interconnected reservoirs being prepared beforehand. The above-mentioned vessels should be applied either separately or being interconnected according to standard microtitrating format. The sample should be mixed with a lyzing buffer and nucleic acids are bound with matrix in closed system including, at least, two interconnected reservoirs. Forced movement of sample's mixture and buffer back and forth from one reservoir into another one for several times through narrow passage provides their thorough intermixing. The method provides quick and safe isolation of nucleic acids.
EFFECT: higher efficiency.
44 cl, 4 dwg, 1 ex
FIELD: medicine, phthisiology, microbiology.
SUBSTANCE: diagnostic material is poured preliminary with chlorohexidine bigluconium solution, homogenized, kept at room temperature for 10-12 h and centrifuged. Precipitate is poured with Shkolnikova's liquid medium, incubated at 37oC for 3 days, supernatant part of Shkolnokova's medium is removed, fresh Shkolnikova's medium is added, and precipitate is stirred and inoculated on the dense cellular egg media. Sensitivity of the strain is determined in 3 weeks by the presence of growth in the control tube only. Invention provides enhancing precision and reducing time for assay. Invention can be used in assay for medicinal sensitivity of tuberculosis mycobacterium.
EFFECT: improved assay method.
FIELD: medicine, biotechnology, pharmacy.
SUBSTANCE: invention relates to agents used for treatment of pathological states associated with disorder of synthesis of neuromediating substances. Method involves the development of pharmaceutical composition and a method for it preparing. Pharmaceutical composition represents subcellular synaptosomal fractions: synaptic membranes, "light" synaptosomes and "heavy" synaptosomes prepared from gray matter of cerebral hemispheres from experimental animals based on the goal-seeking modification of humoral mediators of nerve endings transformed to synaptosomes in development and regression of malignant processes. The composition provides inhibiting the growth of tumor cells, to elevate span-life of patients with ascite Ehrlich's sarcoma, breast adenocarcinoma Ca-755, Wolker's carcinosarcoma-256.
EFFECT: valuable medicinal and anti-tumor properties of composition.
12 cl, 3 tbl, 3 ex
SUBSTANCE: method involves carrying out microscopic examination of blood serum samples taken from femoral vein and cubital vein. Femoral vein sample is taken on injured side. The examination is carried out before and after treatment. The blood serum samples are placed on fat-free glass slide in the amount of 0.01-0.02 ml as drops, dried at 18-30°C for 18-24 h. The set of pathological symptoms becoming larger or not changed after the treatment in comparison to sample taken before treatment, and morphological picture of samples under comparison taken from the cubital vein showing no changes or being changed to worse, the treatment is considered to be effective.
EFFECT: enabled medicamentous treatment evaluation in course of treatment to allow the treatment mode to be changed in due time; avoided surgical intervention (amputation); retained active life-style of aged patients.
FIELD: medicine, clinical toxicology.
SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
EFFECT: higher accuracy of prediction.
2 ex, 3 tbl
FIELD: medicine, juvenile clinical nephrology.
SUBSTANCE: disease duration in case of obstructive pyelonephritis should be detected by two ways: either by detecting the value of NADPH-diaphorase activity, as the marker of nitroxide synthase activity in different renal department and comparing it to established norm, or by detecting clinico-laboratory values, such as: hemoglobin, leukocytes, eosinophils, urea, beta-lipoproteides, lymphocytes, neutrophils, the level of glomerular filtration, that of canalicular reabsorption, urinary specific weight, daily excretion of oxalates, arterial pressure, and estimating their deviation against average statistical values by taking into account a child's age.
EFFECT: higher efficiency of detection.
7 dwg, 1 ex, 6 tbl
FIELD: medicine, urology.
SUBSTANCE: the present innovation deals with differential diagnostics of prostatic cancer and other prostatic diseases at the stage of primary inspection. The method includes the detection of PCA and calculation of probability coefficient for prostatic cancer (PCC) by the following formula: where e - the foundation of natural logarithm (e=2.718…), PCA - the level of total blood PCA in ng/ml, V - patient's age in years. At PCC value being above 0.2 one should diagnose prostatic cancer and to establish final diagnosis one should perform polyfocal prostatic biopsy. The method enables to increase accuracy of diagnostics at decreased number of unjustified prostatic biopsies.
EFFECT: higher efficiency of diagnostics.
FIELD: medicine, biology.
SUBSTANCE: invention relates to nutrient medium used for accumulation of cells for the following cytological and/or immunocytochemical analysis carrying out. Invention relates to medium containing salts NaCl, KCl, anhydrous CaCl2, MgSO4 x 6 H2O, MgCl2 x 6 H2O, Na2HPO4 x 2 H2O, KHPO4, NaHCO3, and also glucose and Henx's solution, 10% albumin solution and polyglucin taken in the ratio 1:1:1. Invention provides enhancing the preservation of cells.
EFFECT: improved an valuable properties of nutrient medium.