Device for non-invasive potentiometric determination of oxidant/antioxidant activity of biological tissues
SUBSTANCE: group of inventions relates to medicine, cosmetology, production of food products, vitamins, food supplements, drugs and describes versions of device for realisation of non-invasive potentiometric determination of oxidant/antioxidant activity of biological tissues, which includes device for measuring potentials and double-sided electrode, made in form of plate with similar working surface, covered with electricity-conducting gel, containing mediator system. Electrodes are fixed on biological tissue in such a way that one working surface, playing role of measuring electrode, is in direct contact with biological tissue via gel, second working surface pale role of comparison electrode. Electrodes contact with each other via gel, with oxidant/antioxidant activity being determined by formulae with application of difference between final and initial potentials.
EFFECT: simplification, as well as increase of accuracy and reliability of determination, is achieved.
14 cl, 3 tbl, 4 dwg
The invention relates to the field of electrochemical methods of analysis, in particular to the analysis of the surface of biological tissues (including skin) to determine the integral oxidant/antioxidant activity of the examined object.
The method for evaluation of antioxidant status of the skin (international publication WO 2007/077360). The rate is measured by voltammetry using a working electrode, reference electrode and counter-electrode, placed in contact with the skin solution.
The disadvantage of this method is the use of direct skin contact of the solution, feeding the potentiostat and current source of information. The latter is not a unique function of the content of antioxidants in the object of study. It depends, along with other, not identifiable parameters also from the presence of impurities, temperature and condition of the surface of working electrode.
The known method of determining the level of carotenoids in the surface layer of the biological tissue as a measure of their antioxidant status. In the basis of measurement is the principle of Raman spectroscopy. Beam of a specific wavelength interacts with molecules of carotenoids, thus there is a shift from blue to green part of the spectrum (U.S. Patent No. 6205354).
The disadvantage of this method is that it assesses only antioxidant activity, due to carotenoids, which are only one species of a large number of antioxidants, in particular, in the skin, i.e. the concentration does not allow to judge the overall antioxidant content in the surface layer of the biological tissue.
The closest technical solution to the claimed invention is a device for noninvasive potentiometric determination of oxidant/antioxidant activity of biological tissues, including open-front casing, in which is placed connected to the device for measuring potentials, electrically interconnected conductive gel containing neurotransmitter system, the reference electrode and the measuring electrode (Patent RF №2433405).
This device has the following disadvantages.
The measurement process when using this device involves many steps: coating the surface of biological tissue in an electrically conductive gel with incorporated neurotransmitter system, the installation on the surface of biological tissue working electrode in contact with the gel containing neurotransmitter system, the introduction into contact with the gel reference electrode and then measuring. The reference electrode is disposable, which increases the measurement process. When gluing the reference electrode on the skin nebo�possible to ensure a tight fit of the measuring electrode to the uneven surface of the object. This entails the penetration of air and, consequently, the formation of air bubbles, which distorts the measurement results. In addition, the known device is complicated in structure, and performance of electrodes made of different materials and of different shapes preclude their interchangeability.
The problem solved by the present invention is to simplify the design and operation of the device, improving performance.
The technical result provided by the present invention is to reduce time in preparation for the measurement, reducing the number of operations in the operation of the device, providing the possibility of reuse, improving the accuracy and reliability of measurement results.
The technical result and the solution of this problem is achieved in that the electrodes are identical in the form of an electrically conductive metal-containing layer deposited on the opposite side of the common dielectric substrate in the form of a plate, on top of the conductive metal-containing layer is a layer of an insulator separating the conductive layer on the contact and a working zone, wherein the gel of transmitter system applied to the working area of the electrodes a uniform layer of uniform thickness not exceeding 0.3 mm. Also the technical result is achieved in that the distance�merger between the outer surfaces of the electrodes is 1.1...2.2 mm; the dielectric substrate is made of high temperature ceramic material; the body is made of flexible material, which preferably use a band-aid on a synthetic basis; the width of the insulator layer is not less than the width of the dielectric substrate plate.
The technical result is also achieved by another embodiment, characterized by the fact that the electrodes are identical in the form of a planar thick-film electrodes on top of the conductive film which is an insulating layer separating a film on contact and working zones, wherein the substrate electrodes are connected to the sides opposite to the surfaces with an electrically conductive film, and the gel of transmitter system deposited on the working areas of both electrodes with an even layer of uniform thickness.
These distinctive signs are essential and together provide the achievement of the technical result.
The implementation of identical electrodes on a common substrate, or connected reverse-conductive by the parties provides a simplified design and operation, increases the accuracy and reliability of measurements due to the exclusion of factors associated with impact on the processes of measurement of different materials, and the arrangement of working areas of the electrodes on opposite sides of the p�Daiki prevents diffusion of the test medium in the area of the reference electrode. This feature also simplifies the operation of the device. The execution of the body of flexible material, preferably plaster, allow to easily adapt the device to uneven surfaces, eliminate the formation of air bubbles between the gel and the body, and also provides a more tight fit of the gel and electrode to the sample surface. The use of adhesive plaster in combination with two-sided design is identical electrodes greatly simplifies and accelerates the process of preparation for the measurements, which minimizes the influence of external environment on the neurotransmitter system in the gel and, consequently, improves the accuracy of the measurements. The stated thickness of the gel and the distance between the surfaces of the electrodes of the selected optimal according to the results of research from the perspective of minimizing the adverse impact of processes and measurement time, which also increases the accuracy and reliability of measurement results.
Fig. 1 shows the device in General.
Fig. 2 shows a view of the electrodes in the plan.
Fig. 3 shows a view of the electrodes from the side.
Fig. 4 shows the electrodes according to the second embodiment of the invention, implemented on separate substrates.
The device for noninvasive potentiometric determination of oxidant/antioxidant activity of biological tissues contains the reference electrode 1 and edit�measuring electrode 2, made identical in the form of an electrically conductive metal-containing layer 3 deposited on the opposite side of the common dielectric substrate in the form of a plate 4. The distance between the outer surfaces of the electrodes is 1.1...2.2 mm. the Dielectric substrate is made of high temperature ceramic material. On top of the conductive layer 3 is an insulating layer 5 separating the electrically conductive layer 3 on pin 6 and 7 working zone. The width of the insulator layer 5 is not less than the width of the plate 4 dielectric substrate. On work areas 7 of the electrodes 1 and 2, uniform layer of uniform thickness less than 0.3 mm applied conductive gel 8 of transmitter system. Work areas 7 of the electrodes during the measurement are located in the housing 9, is placed with the open side on the studied object, made of a flexible material, preferably of plaster with a synthetic substrate. The contact areas 6 are electrically connected with the device for measuring potentials 10. The device is placed on the examined object 11.
According to the second embodiment of the invention, the electrodes 1 and 2 is made as a separate planar thick-film electrodes on top of the conductive layer 12 which is an insulating layer 13 separating the electrically conductive layer to the contact 14 and 15 working zone. The substrate 16 of the electrodes 1 and 2 soy�inany between the parties, opposite surfaces with an electrically conductive layer 12.
The device operates as follows.
Plate 4 with the electrodes 1 and 2, is covered with a conductive gel 8 with incorporated neurotransmitter system, is mounted on the surface of biological tissue 11, in particular of the skin, with adhesive plaster, performing the functions of the housing 9 of the device. Due to the flexibility of the hull ensures a snug fit of the device to a surface of any shape without the formation of air bubbles and air access to the gel 8. The necessary condition is the presence of work zones 7 of the electrodes 1 and 2 in contact with a conductive gel 8. Established in the system of electric potential measured by the device 10. The change in potential difference is fixed from the moment of contact of the gel and electrode with the object and before the expiration of 5-15 min.
Oxidant/antioxidant activity is determined using the difference between the final and initial potential by the formulas:
where Δ - the difference between the initial potential of the system and the value of the potential established at the end of the measurement, COx- concentration of the oxidized form of the mediator system, M; CRed- the concentration of reduced forms of the mediator system, M; AOA - antioxidant activity, mEq; OA - oxidant activity, mEq.
�length of the leg between the outer surfaces of the electrodes, which is 1.1...2.2 mm provided that the thickness of the gel on the working surfaces of the electrodes is not more than 0.3 mm is optimal, since, on the one hand, creates the conditions under which during the measurement of oxidants/antioxidants from the research object do not reach the working surface of the electrode, and on the other hand, ensures a minimum time of establishment of the stationary state diffusion processes in the gel and, consequently, leads to the correct measurement of capacity.
When the thickness of the layer of gel between the skin and the working electrode than 0.3 mm increases the diffusion time of the designated substances to the working electrode, which leads to a significant increase in measurement time. When the distance between the conductive surfaces of the electrodes is less than 1.1 mm-defined objects (oxidants/antioxidants) of time during the measurement to prodifferentiating to the surface of the electrode, thus distorting the measurement results. When the distance between the conductive surfaces of the electrodes is more than 2.2 mm increases the electrical resistance between them, which makes a significant error in the measurement result.
The dependence of the precision (error) of measurement of the thickness of the layer of electrically conductive gel and the distance between the outer surfaces of the electrodes are shown by measurement of the AOA model system�we electroconductive gel, containing neurotransmitter system and ascorbic acid at a concentration of 2×10-5M-EQ. The measurement time is 10 minutes.
Measurement error depending on the thickness of the layer of electrically conductive gel are shown in table 1.
As can be seen from table 1, with increasing thickness of the layer of gel on the electrodes increases the accuracy of measurement results. At a thickness of more than 0.3 mm, it significantly increases and becomes unacceptable - more than 15%.
Measurement error depending on the distance between the conductive surfaces of the electrodes are presented in table 2.
The measurement results show that when the distance in the range of 1.1...2.2 mm the error is in acceptable limits and does not exceed 10%, varying proportionally to the predetermined distance. If the distance is outside the specified range of distances significantly increase the magnitude of the measurement error and the spread of values.
The device was used to evaluate the oxidant-antioxidant activity of the skin in groups of patients both healthy and suffering from various diseases. Table 3 shows sample results of such measurements with the aid of the inventive device (the number of Parallels n=4-5).
As can be seen from the prima�s, the device provides a reliable assessment of oxidant/antioxidant activity of human skin depending on the condition of the body.
The proposed device, providing accurate and precise determination of oxidant/antioxidant status of live biological tissues, greatly simplifies and cheapens the process of measurement.
1. The device for noninvasive potentiometric determination of oxidant/antioxidant activity of biological tissues, including open-front casing, which are connected to the device for measuring potentials, electrically interconnected conductive gel containing neurotransmitter system, the reference electrode and the measuring electrode, characterized in that the electrodes are identical in the form of an electrically conductive metal-containing layer deposited on the opposite side of the common dielectric substrate in the form of a plate, and on top of the conductive metal-containing layer is an insulating layer separating the electrically conductive layer is in contact and working areas, the gel of transmitter system applied to the working area of the electrodes a uniform layer of equal thickness.
2. The device according to claim 1, characterized in that the thickness of the layer of gel on the working areas of the electrodes is not more� 0.3 mm.
3. The device according to claim 1, characterized in that the distance between the outer surfaces of the electrodes is 1.1...2.2 mm.
4. The device according to claim 1, characterized in that the dielectric substrate is made of high temperature ceramic material.
5. The device according to claim 1, characterized in that the casing is made of flexible material.
6. Device according to claims.1, 5, characterized in that as the material of the body using a band-aid on a synthetic substrate.
7. The device according to claim 1, characterized in that the width of the layer of the insulator is not less than the width of the dielectric substrate plate.
8. The device for noninvasive potentiometric determination of oxidant/antioxidant activity of biological tissues, including open-front casing, which are connected to the device for measuring potentials, electrically interconnected conductive gel containing neurotransmitter system, the reference electrode and the measuring electrode, characterized in that the electrodes are identical in the form of a planar thick-film electrodes on top of the conductive metal-containing layer which is a layer of an insulator separating the conductive metal-containing layer on the contact and working zones, wherein the substrate electrodes are connected storo�AMI, opposite surfaces with an electrically conductive layer, and the gel of transmitter system deposited on the working areas of both electrodes with an even layer of uniform thickness.
9. The device according to claim 8, characterized in that the thickness of the layer of gel on the working areas of the electrodes is not more than 0.3 mm.
10. The device according to claim 8, characterized in that the distance between the outer surfaces of the electrodes is 1.1...2.2 mm.
11. The device according to claim 8, characterized in that the substrate electrode is made of high temperature ceramic material.
12. The device according to claim 8, characterized in that the casing is made of flexible material.
13. Device according to claims.8, 12, characterized in that as the material of the body using a band-aid on a synthetic substrate.
14. The device according to claim 8, characterized in that the width of the layer of the insulator is not less than the width of the substrate electrodes.
SUBSTANCE: testicular germ cells are measured quantitatively. That is ensured by 50-day oral administration of selexen and ascorbic acid into male white rats in doses 1.5 and 500 mg/kg of animal's body weight respectively once a day. 14 days later, administering the selenium-containing biocomplex is accompanied by the 30-minute daily exposure to microwave radiation at 42 GHz (λ=7.1 mm) for 30 days. Once the experimental exposures are completed, the corrective properties of the biocomplex as having an effect on the morphofunctional state of epididymal sperm cells are assessing by formula: MFSI=A+B, wherein MFSI is a morphofunctional state index, A is a portion of normal sperm cells in relation to the reference, and B is a portion of moving sperm cells in relation to the reference. If the MFSI value is 1.3 or more, the spermatogenesis correction is considered to be ineffective, while the MFSI value being more than 1.3 shows the effective spermatogenesis correction if exposed to microwave radiation.
EFFECT: invention enables assessing the spermatogenesis correction efficacy with underlying administration of the biocorrector.
2 tbl, 3 dwg, 3 ex
SUBSTANCE: test tray comprises a case 1 made of an optically transparent material. From one end face, the case 1 has a stop plug 2 with a hole 3, a connecting pipe 4 threaded to connect to either a haemofilter 5, or a cap 7. The case 1 comprises a movable piston 8 connected by a rod 10 to a handle 11. Electrically supplied electrodes 12 are arranged on surfaces of the stop plug 2, piston 8 and on the inner surface of the case 1. The electrodes 12 are connected to contact groups of a device - a laser analyser - through conductors 13, 14. What is disclosed is an alternative version of the structural embodiment of the test tray.
EFFECT: sterile measurement of the colloidal fluid sample.
17 cl, 5 dwg
SUBSTANCE: technique involves the three-stage diagnosis of all the patients suffering tumour diseases of various localisations. The first stage involves CT-densitometry performed every 6 months; if the CT-densitometry shows sites with varying spine bone density by 30% and more, the second diagnostic stage that involves a transpedicular biopsy is initiated. If the biopsy material appears to contain no tumour material, the third diagnostic stage starts with F-18 positron-emission tomography (PET-CT).
EFFECT: improving the early diagnosis of the spinal tumours.
SUBSTANCE: clinical assessment of the oral mucosa state is ensured by examining non-stimulated oral fluid or swabs. That involves determining five parameters: content of yeast-like fungi Candida in the yeast or mycelia form (1), concentration of secretory immunoglobulin A (SIgA) (2) and lysozyme (3), emission light sum (S) 5 minutes before chemoluminescent examination (4); the Wood-beam beam luminescent examination covers marginal portions of the gum and apexes of interdental papillas, buccal mucosa within dental occlusion, dorsal surface of the tongue within the thread-like processes, and performing morphological examination of the multilayer squamous epithelium of the buccal mucosa along the dental occlusion line (5). The derived results enable diagnosing the absence of pathogenic microflora and oral mucosa pathology, Candida carriage or chronic oral candidiasis in the mycelia or yeast form in the form of hyperkeratosis or leukokeratosis.
EFFECT: using the invention enables increasing the differential diagnostic accuracy for keratolytic processes in the form of white manifestations.
10 dwg, 5 ex
SUBSTANCE: method includes selection, and crushing of biomaterial, two-stage extraction of pesticides with n-hexane, purification of biomaterial from coextractive substances with concentrated sulphuric acid, formation of concentrate of n-hexane extract of pesticides, drying, sample formation by dissolution in 0.5-1 ml of n-hexane and carrying out gaschromatographic identification.
EFFECT: invention is characterised by higher effectiveness and accuracy of research and can be used in biology, ecology, medicine for gaschromatographic identification of organochlorine pesticides, namely α-HCCH, β-HCCH, γ-HCCH, DDT, DDD, DDE, in various biomaterials, such as lipids of internal organs and tissues, blood, milk, bird feathers.
2 cl, 1 dwg
SUBSTANCE: invention represents a method for preclinical study of cardiotropic antiarrhythmic drugs, involving determining the bioelectric parameters in isolated multicellular perfused preparations and measuring an action potential duration, differing by the fact that the isolated multicellular perfused preparations are presented by rat's pulmonary vein myocardium; the parameters are measured in three operation modes of the multicellular preparations; a resting potential is additionally measured; varying APD 90%, related APD 50%/APD 90%, a spontaneous shear velocity of the resting potential, the most positive membrane potential in the resting preparation, a spontaneous activity train repetition rate, spontaneous action potential train repetition and variability frequency, post-depolarisation number and intensity, as well as a shear membrane potential corresponding to the beginning of train activity are used to evaluate the signs of antiarrhythmic and arrhythmogenic action.
EFFECT: more reliable prediction of the antiarrhythmic action of the potential pharmacological agents and reduction of experimental phase time.
FIELD: measurement equipment.
SUBSTANCE: invention comprises a method of determination of nano trace contaminants, supposing the use of emulsion from liquid crystal drops, dispersed in water and capable to change a configuration of liquid crystal drops in presence in the emulsion composition of foreign admixtures, measurement of change of intensity of light scattered by the emulsion, according to which it is possible to evaluate the content and density of required admixtures differing by that for a liquid crystal the compounds capable to trans-cis-transition under the effect of actinic light are selected and before measurement of change of light intensity the emulsion is illuminated additionally by actinic light, thus ensuring change of configuration in liquid crystal drops due to trans-cis-transition in liquid crystal molecules.
EFFECT: increase of sensitivity of method of determination of nano-trace contaminants.
SUBSTANCE: invention refers to a method for identifying living and dead mesozooplankton in seawater samples, which involves taking samples, staining the organisms with suitable colouring material, giving a visual estimation of the colour intensity of the units under the microscope, which is combined with microphotographying the units with an adjustable camera without changing the settings keeping throughout a photographic session of at least one sample; thereafter colour and brightness specifications average for each unit are measured in the formed images with using a painting program, e.g. Adobe Photoshop package, and the units are referred to living or dead by a discriminative analysis of the varied digital values.
EFFECT: improving the method.
SUBSTANCE: identification method of blue pus bacillus Pseudomonas aeruginosa involves inoculation of the test material on a hard substrate, incubation of the inoculum under anaerobic conditions at the temperature of 37°C during 16-18 hours. The obtained bacterial mass in the amount of one bacteriological loop is placed in 300 mcl of a physiological solution and warmed-up at the temperature of 98-99°C during 20-30 minutes and centrifuged at 12000 revolutions per minute during 30 seconds. To a supernatant there added is colouring material for electrophoretic detection in the quantity of 0.5 mcl and 20 mcl is added to a well with a size of 4×1 mm 1.2% agarose gel on TAE-buffer with 10 mcl of 1% ethidium bromide. Besides, the amount of 3 mcl of the solution of standard DNA-marker 1 kb containing DNA fragments in the range of 250-10000 bp is added to the test well. Horizontal electrophoresis is performed during 15-20 minutes, and at detection on the obtained electrophoregramme of the test bacterial mass of three strips radiating in ultraviolet light, one of which corresponds to fragments of standard DNA-marker with the size of 10000 bp, the second one corresponds to fragments of standard DNA-marker with the size of 6000-8000 bp and the third strip at the end of the track in the form of a whisk, which corresponds to fragments of standard DNA-marker with the size of less than 750 bp, there identified is Pseudomonas aeruginosa in the test bacterial mass.
EFFECT: method allows quick and complete identification of pigment-shaping and non-pigment strains Pseudomonas aeruginosa in a test bacterial mass.
5 dwg, 2 ex
SUBSTANCE: for analysis of removal with meadow grass of biochemicals the yield fluctuations are accounted depending on the structure of phytocenosis in the form of a ground cover. Conducting statistical data processing of testing the samples of grass from the test plots on the meander, central and terrace near flood plains, and also on the meadows with uneven and tiling location of grass species. And prior to establishment of sample plots the terrain reconnaissance is carried out with the grass cover selected for the measurements, an outline map of location of the components of the grass cover is made, then on each component of the grass cover in the form of a plot at least one temporary test plot is established, the wet and air-dry weight of the sample is determined by weighing on the cut grass sample. At that the tests on biochemical analysis are additionally carried out on the dried samples of grass to determine the concentration of at least three chemical nutrients in the form of mobile nitrogen, potassium and phosphorus oxides, and then by summing the concentrations of these three substances the total summarised removal of substances from the aerial part of grass on all test sites is calculated.
EFFECT: invention enables to determine the productivity of meadows.
9 cl, 12 dwg, 4 tbl, 1 ex
SUBSTANCE: method of determining the diffusion coefficient of solvents in massive products from orthotropic capillary-porous materials consists in the creation in the sample under analysis of an even initial content of a solvent distributed in the solid phase , bringing the flat sample surface in contact with a solvent dose source, measuring the change of a galvanic converter signal in time, determination of the time of reaching the maximum on the curve of the galvanic converter EMF change and the calculation of the diffusion coefficient, pulse influence on the flat surface of the product under analysis with the dose of the solvent is realised on a straight line in a specified direction of an orthotropic material, electrodes of the galvanic converter are made in the form of rectilinear segments which are placed from both sides of the line of the pulse influence on straight lines, parallel to the line of the pulse influence and located at an equal specified distance from it, and the required coefficient is calculated by a specified formula.
EFFECT: providing the possibility to control the accuracy increase and determination of the diffusion coefficient in different directions of the orthotropic capillary-porous material.
SUBSTANCE: method provides blood serum analysis by a bipolar method for multi-frequency impedancemetry to determine impedance modulus (|Z|) and phase angle (φ) at AC low-intensity frequencies 20, 98, 1000, 5000, 10,000, and 20,000 Hz by means of hardware-software complex provided with BIA-lab Compositum software; measurements are taken in a microchamber of 50 mcl; the software automatically calculate the blood serum concentrations of total protein, glucose, chlorides and bivalent ions on the ground of solution of assembled mathematical equations; the result is displayed and can be printed.
EFFECT: higher diagnostic effectiveness by eliminating the need for using chemical agents, reducing the time for analysis, cutting the cost and extending the indications for using the technique.
SUBSTANCE: invention relates to the field of analytical chemistry and can be used for automatic or express-analysis in laboratory or industrial conditions. The method of controlling main components of a chloroaluminate melt includes the determination of the molar ratio of the said components in the liquid chloroaluminate melt by a potentiometric method; measurement of the potential difference is carried out between a working electrode of aluminium and an aluminium comparison electrode both located in an electrolyte , with the comparison electrode being separated by a diaphragm from the main melt and representing an assembly from a ceramic casing and aluminium wire, submerged into the located in a casing chloroaluminate melt, saturated by solid potassium chloride, with the molar ratio of the main components in the melt being determined by the value of difference of potentials between the electrodes by means of preliminarily built calibration curves in coordinates "difference of potentials - ratio of concentrations of potassium chloride to aluminium chloride".
EFFECT: compactness of the installation, cheapness of the applied material and simplicity of technological equipment, applied for the said method realisation.
2 cl, 2 ex, 2 dwg
SUBSTANCE: invention relates to the electrochemical processor which comprises: a) first electrode and the second electrode, each of which has the first and opposite second surfaces, and the first electrode and the second electrode have the differing electrode potentials and physically separated mutually along the axes X, b) electrolyte, which covers at least a part of the first surface of the first electrode and a part of the first surface of the second electrode along the axis Y and electrically connects the named first electrode with the second electrode. Meanwhile at least a part of the first surface of the second electrode not covered by electrolyte, is covered towards the axis Y with a an electric insulation material, which bounds with an electrolyte, and the activation of the electrochemical processor favours to lateral foliation of the second electrode and isolation material, starting from the place of contact of the second electrode and isolation material, that ensures the channel between the second electrode and the isolation material. Besides, the invention relates to the use of this electrochemical processor and method of assembly of such electrochemical processor.
EFFECT: processor ensures the constant time of reaction.
17 cl, 12 dwg
SUBSTANCE: invention relates to analytical chemistry. The method is characterised by electrochemically concentrating benzoic acid on the surface of a graphite electrode for 90 s at electrolysis potential of (-0.500) V on a background of 0.1 mol/l sodium hydrogen phosphate, recording polarisation curves with linear potential sweep rate of 25 mV/s and determining concentration of benzoic acid from the peak height in potential range of 0.5-1.6 V relative to a silver chloride electrode.
EFFECT: method provides highly sensitive and rapid determination of benzoic acid in medicinal drugs.
3 ex, 6 tbl
SUBSTANCE: device for determining the oxygen and hydrogen concentration in a gas medium relates to measurement instruments and can be used for control of gas media parameters, in particular of those, containing oxygen and hydrogen. The device consists of a channel (7), located at an angle to the horizon, an input hydrogen sensor (2) and an input oxygen sensor (3), located in the input part of the channel (7) cavity, an input catalytically active element (1), installed in the channel (7) cavity above the output sensors of hydrogen (2) and oxygen (3), an output hydrogen sensor (5) and an output oxygen sensor (6), located in the channel (7) cavity between the input (1) and output (4) catalytically active elements. The input (2) and output (4) catalytically active elements are made of highly porous cellular materials with a platinum coating applied on their surface. As the input hydrogen sensor (5) and the output hydrogen sensor (7) used are solid electrolyte sensors of the hydrogen concentration with a ceramic sensitive element, made of oxygen-conducting ceramics.
EFFECT: increased speed of the device performance and sensitivity, provision of protection against erroneous readings of the device.
3 cl, 1 dwg
SUBSTANCE: invention relates to the field of analytic chemistry an can be applied for the simultaneous determination of the content of Cu(II), Pb(II), Fe(III) and Bi(III) ions in different matrices. The essence of the invention: a method of the combined determination of Cu(II), Pb(II), Fe(III) and Bi(III) ions by a method of capillary zone electrophoresis includes preliminary complex-formation of the components of a sample with ethylenediaminetetraacetic acid, introduction of the sample zone into a non-modified quartz capillary, filled with a phosphate buffer electrolyte, after a zone of diglycylglycine, separation of the components at negative polarity of a voltage source, direct spectrophotometric detection at a wavelength of 260 nm, identification and quantitative determination of each component by a preliminarily built calibrating diagram or by a method of standard additives.
EFFECT: extended list of determinable components, development of a simple, sensitive and express method of determining ions of transition metals in environmental objects, wastes of industries, complex oxide materials and other objects by the method of capillary zone electrophoresis.
FIELD: measurement equipment.
SUBSTANCE: invention relates to the field of engineering, which can perform remote monitoring of formation and growth of cracks in steel structures. A device includes a cover that has magnetic legs for attachment of the cover to a ferromagnetic structure, at least one pair of microprocessor-controlled voltage controls; with that, each voltage control has a wire of a sensor to an electrochemical fatigue sensor attached to the structure subject to an analysis for availability of cracks as a result of fatigue of metal in the steel structure, a power source and earthing; the voltage control is used to perform monitoring of fatigue state of the steel structure; each voltage control is electrically isolated from the rest part of an electrical mounting plate of the device and includes an analogue-to-digital converter. Voltage is applied by means of a voltage control through the above sensors during cyclic loading, and current flowing through the above sensors is measured, and the measurement result is digitised for the analysis of fatigue state of the steel structure.
EFFECT: possible prevention of occurring noises and distortion of data during the measurement.
34 cl, 4 dwg
SUBSTANCE: invention relates to the field of electrochemical methods of analysis, in particular to the analysis of solutions to determine the total antioxidant/oxidant activity. The invention can be used in research laboratories, food industry, medicine to determine the antioxidant/oxidant activity of natural, synthetic and biological objects to study antioxidant/oxidant properties of substances and products, control the composition of food products, and diagnose diseases. The essence of the claimed method consists in the following: determination of the antioxidant/oxidant activity is carried out by a difference of potentials, one of which is measured after the realisation of a chemical reaction between antioxidants/oxidants of an analysed substance and an applied agent, and the second one - after the following addition of a solution of a reagent or the analysed substance.
EFFECT: invention provides an increase of accuracy, reliability and reproducibility of results, increase of the analysis expression, extension of the range of analysed substances, applied reagents and solvents.
3 cl, 6 dwg, 6 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine and represents a reagent for detecting glucose containing an enzyme FAD-glucose dehydrogenase, a phenothiazine or phenoxazine mediator, at least one surfactant, a polymer and a buffer. The reagent is used with an electrochemical test sensor comprising a number of electrodes with the test sensor possessing high filling rate and accuracy.
EFFECT: reagent is stable and characterised by lower background current of the test sensors.
19 cl, 2 tbl, 13 dwg, 9 ex
FIELD: biology, experimental medicine.
SUBSTANCE: the method deals with filling hermetically sealed, optically transparent by height active capacity of a chamber with suspension of abiotic microobjects prepared upon distilled or bidistilled water and supplying opposite-charge tension of the same power onto two plane-parallel electrodes followed by registering the amplitude of fluctuation in vision field of light microscope's ocular.
EFFECT: higher efficiency of measurement.
1 ex, 1 tbl