Method for prediction of preeclampsia in second trimester of pregnancy
SUBSTANCE: invention represents a method for the prediction of preeclampsia in the second trimester of pregnancy by blood examination, differing by the fact that the activity of acid and neutral proteinases is measured in blood serum of the women 7-8 weeks pregnant; if the activity of acid proteinases is more than 5.6 mcmole/l, while the activity of neutral proteinases is more than 3.9 mcmole/l, the preeclampsia progression in the second trimester of pregnancy is predicted.
EFFECT: higher accuracy and specificity of the method for the prediction of gestational toxicosis.
The invention relates to the field of medicine, namely to obstetrics, and can be widely used to objectively predict the development of preeclampsia (preeclampsia) in the second trimester of pregnancy.
Previously used the term "preeclampsia" is currently in compliance with the approved MoH standards and protocols of the Scientific center of obstetrics, gynecology and Perinatology recommended from 2012 to replace the term "preeclampsia". Because the distinction between preeclampsia and preeclampsia only terminological, and the replacement of the term is relatively recent, in some analogs, to which we refer, uses the term - gestosis.
Despite the undoubted successes in the treatment of preeclampsia (gestosis) she remains a frequent and life-threatening complications of pregnancy and childbirth, leading to health problems in the mother, fetus and newborn. In the structure of maternal mortality in Russia this pathology occupies the third place, its specific weight is 11.8-14.8 per cent (Torchinov A. M., tsakhilov S. G., Arahova D. H. and others the Relevance of pre-eclampsia (preeclampsia) in modern obstetrics. Problems and solutions (literature review) // Probl. Reprod. - 2010. - №3. - 87-91). Pathogenic (pathochemical and pathophysiological mechanisms laid down already in the early stages of pregnancy and due to a violation of invasion of triabl�hundred and gestational transformation of the spiral arteries of the uterus with subsequent formation of deep metabolic damage in the system mother-placenta-fetus.
To date, the early prediction of pre-eclampsia (preeclampsia) during different periods of gestation remain completely unresolved, which makes timely preventative therapy for this obstetric pathology.
Among well-known in the patent and scientific and medical literature methods for the prediction of preeclampsia include the following.
Known method for predicting preeclampsia (patent RF №2249212, 27.03.2005) by examining the blood plasma of the pregnant woman, characterized in that in the early stages (from 5 weeks of gestation) determine the level of carbonyl derivatives of proteins and when the value is equal to 51.7% of the optical density /g of protein and higher predict the development of preeclampsia.
The increase in the number of carbonyl derivatives linked to the degradation of proteins due to the development of oxidative stress, which also occurs when growth retardation (I. Mert, A. Oruc S, Yuksel S. et al. Role of oxidative stress in preeclampsia and intrauterine growth restriction // J. Obstet. Gynaecol. Res. - 2012. - Vol.38, N 4. - P. 658-664), which indicates its lack of specificity.
Known method for predicting preeclampsia mild severity from early pregnancy through the study of peripheral venous blood of women, characterized in that in the period from 6 to 12 weeks of gestation examine the relative content of CD3+CD16+ lymphocytes and its values, Rav�s or more than 5.4%, predict the development of preeclampsia mild severity (patent RF №2265221, 27.11.2005).
The disadvantage of this method is the limited possibility to predict only mild preeclampsia less hazardous to the mother, the more pronounced degree.
According to the patent of Russian Federation №2287823 (20.11.2006) predict the occurrence of preeclampsia by analysis of blood serum of a pregnant, characterized in that in the serum to determine the concentration of antibodies to neurospecific enolase and gliofibrillary acidic protein and the level of anti-NDE-antibodies more than 0.6 μg/ml or anti-GFAP antibodies more than 0.9 μg/ml predict the development of preeclampsia.
In the patent of Russian Federation №2304783 (20.08.2007) proposed a method for predicting the development of preeclampsia by examining the peripheral blood of the pregnant woman, characterized in that starting from 22 weeks of pregnancy in serum to determine the concentration of neurospecific enolase and gliofibrillary acidic protein and the magnitude of neurospecific enolase above and 12.4 ng/ml and gliofibrillary acidic protein above 4 ng/ml predict the development of preeclampsia.
These methods are difficult to meet in an obstetric hospital, because require imported special equipment and expensive reagents. In addition, the research is conducted only with the end of the second trimester of his pre�STI, hence the impossibility of an earlier prediction of preeclampsia.
A method of predicting the occurrence of preeclampsia in pregnant women (Patent RF №2478960,10.04.2013), including the study of blood serum by enzyme immunoassay in terms of 16-18 weeks of gestation, characterized in that determine the content of neopterin concentrations and the occurrence of preeclampsia predict when neopterin levels above 8.1 nmol/L.
For this method the characteristic drawbacks of the previous method, it should be noted also that the content of neopterin is a marker activity of cellular immunity, indicator of inflammatory process, changes in a number of pathological conditions (E. A. Sviridov, Telegina T. A. Neopterin and its reduced forms: biological role and involvement in the cellular immune system // advances in biological chemistry. - 2005. - V. 45. - Pp. 355-390).
Known method for predicting preeclampsia (patent RF №2485894, 27.06.2013), including the identification of endothelial function in early pregnancy, characterized in that in the first half of pregnancy twice in a period of 5-7 weeks and 16-19 weeks assess the functional activity of the endothelium on the results of cuff compression test on the brachial artery of the mother and compute the indicator photocatalysis dilatation (PPD) of the brachial artery after the first and second research �about the formula, and if the indicator IFE decreases or increases by less than 4% relative to the initial values in the first study, predict the development of preeclampsia, and if the indicator IFE increased by 4% and more in comparison with initial values, predict uncomplicated pregnancy without clinical manifestations of preeclampsia.
The disadvantages of this method include the need to double examination of the pregnant woman and the nonspecific character, since the index photocatalysis dilation can be changed and at such a complication of gestation as placental insufficiency, the development of which is also associated with a modification of the functional activity of the endothelium (Zainulina M. S. the Role of endothelial dysfunction in the pathogenesis of placental insufficiency // Regional circulation and microcirculation. 2003. - No. 2. - P. 36-42).
According to the patent of Russian Federation №2481578 (10.05.2013) predict the development of severe preeclampsia with blood, characterized in that a pregnant second trimester count leukocyte index of intoxication and when the value above 1.6 predict the development of severe preeclampsia.
The disadvantage of this method is later than the determination of a prognostic indicator only in the second trimester, the increase above guideline values in this period are considered as risk time�itia severe preeclampsia. Final diagnosis: severe preeclampsia is exposed only in the third trimester. In addition, the present method predicts the development of severe preeclampsia.
In all these patents are no data on the accuracy of the method.
The prototype of the claimed invention, the selected method for predicting preeclampsia (patent RF №2263913, 10.11.2005) by examining peripheral venous blood of women, characterized in that in the period 6-13 weeks of gestation to determine the activity of cathepsin D and its value is equal to or more than 0.021% act. f/h predicted the development of preeclampsia in the 3rd trimester.
This method, like the previous one, has a number of drawbacks:
the limited information content of the method: the use of only one enzyme from the group of acidic lysosomal endopeptidases;
- lack of accuracy and specificity of the method;
- the inability to predict an earlier development of gestosis;
- the authors do not indicate which are the reaction products catalyzed by cathepsin D, they are registered at 280 nm, therefore, lead quantities in relative rather than absolute units of the analyte.
These shortcomings are proposed to be eliminated in the present method.
Object of the invention is to develop a highly informative way to 7-8 weeks of pregnancy to make forecasts.�foresight for the development of preeclampsia (preeclampsia) in the second trimester.
The problem is solved as follows: in the serum of women in 7-8 weeks of pregnancy determine the activity of acid and neutral proteinases and when the value of the activity of acid proteinases above 5.6 mmol/l and the activity of neutral proteinases above to 3.9 mmol/l predict the development of preeclampsia (preeclampsia) in the second trimester of pregnancy.
The technical result obtained during the use of the invention is to improve the accuracy and specificity of the method for predicting preeclampsia that will allow time to appoint adequate pathogenetic therapy.
The novelty of the proposed method lies in the fact that the first proposed to predict the development of preeclampsia (preeclampsia) in the second trimester of pregnancy for a single definition in the serum of women in 7-8 weeks of pregnancy, the activity of acid and neutral proteinases.
In the complex chain of violations, leading to the development of preeclampsia (preeclampsia), the importance of the violation of the balance between anabolism and catabolism of proteins that play a key role in all regulatory cellular reactions (Whitford D. Proteins: Structure and Function. - West Sussex, England: John Wiley & Sons Ltd., 2005. - 542 p.). Shift this balance may be accompanied by profound biochemical changes in the maternal organism, and fetoplacental system (Linear�relova T. N., Linda V. A. Metabolism of the placenta and molecular mechanisms of its regulation. Clinical and experimental study. - LAP LAMBERT Academic Publishing, 2012. - 200 C.). Significant place in these processes is a proteinase that plays a Central role in the biological processes of decay and renewal of protein molecules. And only the combined action of acid and neutral endo - and ectoprotheses, functioning as in the lysosomes and other subcellular fractions: mitochondria, microsomes, nuclei and the cytosol, fully regulates the metabolism of proteins (Dean R. the decay Processes in the cell. - M.: Publishing house "Mir", 1981. - 120 p.).
Simultaneous determination of serum acid and neutral proteinases increases the accuracy and specificity of the method and allows you to more objectively predict the development of preeclampsia is still at a preclinical stage. The ability to predict the development of obstetric pathology in the second trimester has undoubted practical value (compared with the forecast of its development in the third trimester) because the rate of complications with the development of preeclampsia in the period 1.5-3.4 times higher than when it is a late manifestation (Savelyeva G. M., Krasnopolsky, V. I., Strizhakov A. N. etc. What is the classification of gestosis (preeclampsia) should adhere to the doctor's clinical practice? // Questions of gynecology, obstetrics and �perinatologia. - 2013. - No. 2. - P. 5-8).
We have established prognostic parameters of the development of obstetric pathology in the second trimester by definition in the serum at 7-8 weeks of pregnancy, the activity of acid and neutral proteinases and acid proteinases above 5.6 mmol/l and the activity of neutral proteinases above to 3.9 mmol/l predict the development of preeclampsia.
Detailed description of the method and examples of its clinical use.
The method is as follows.
Pregnant women to predict the possible development of complications of pregnancy, including preeclampsia, in the morning on an empty stomach produce blood from the cubital vein.
To 0.2 ml of serum is added as a protein substrate of 0.2 ml of 1% albumin in 0.1 M Tris-HCl buffer (pH 7.8) and incubated for 30 minutes at 38°C. stop the Reaction by adding 0.2 ml of 5% solution of trichloroacetic acid, after which the samples centrifuged at 3000 Rev/min In the control sample protein-substrate is added after stopping the reaction. To determine the number of centrifuges formed by proteolysis of tyrosine classical Lowry method using Folin reagent. Sample colorimetric in the visible region of the spectrum at 750 nm. The activity of neutral proteinases is defined as the difference of optical density between the experimental and control samples and expressed mcmol/L. Determination of acid proteinases and their activity calculation performed in a similar manner, using as substrate the reaction of 1% solution of hemoglobin in the sodium acetate buffer (pH 3.5). Calculation of the content of tyrosine spend on calibration graphs constructed using standard solutions of amino acids (ICN, Belgium).
The performance of the proposed method is confirmed by the following clinical examples.
Example 1. Patient B-Eva, 24 years old. Surveyed in the consultative outpatient clinic RNIUP. Perfoermance. Pregnancy 7 weeks, suspected ectopic pregnancy. When ULTRASOUND examination and analysis of HCG in the blood this diagnosis was excluded. The woman has also been conducted on the determination of serum activity of acid and neutral proteinases. The activity of acid proteinases amounted to 5.7 mmol/l, neutral to 4.0 μmol/l, which, according to the claimed method, showed an opportunity for the development of preeclampsia. Pregnant woman refused hospitalization for preventive therapeutic interventions. At 20 weeks gestation, the woman was admitted to the Department of pathology of pregnancy. Diagnosis: preeclampsia moderate severity (edema, BP 140/90). A patient treatment of pre-eclampsia. At the end of therapy was discharged in satisfactory condition. The pregnancy is completed�plastic surgery in full-term childbirth. Thus, the diagnosis of the claimed method was confirmed.
Example 2. The patient-ova, 23 years. Surveyed in the consultative outpatient clinic RNIUP. Avtomobilnaya. Diagnosis: pregnancy 8 weeks. Anemia. After the standard survey, also carried out the determination in the serum activity of proteinases. The activity of acid proteinases was 5.6 mmol/l, the activity of neutral to 3.9 mmol/L. These values of activity indicate the absence of biochemical signs of pre-eclampsia.
Follow-up of a woman until the end of pregnancy, culminating in the full-term birth of a child, confirmed the lack of development of preeclampsia.
According to the claimed method were examined 95 women at 7-8 weeks of gestation in serum determined the activities of acid and neutral proteinases. Of the screened patients in 34 the activity of acid proteinases was 5.6 mmol/l or below, the activity of neutral to 3.9 mmol/l and below. Surveillance in this group of pregnant women for the development of preeclampsia is not revealed. In 61 patients, these figures were respectively above 5.6 mmol/l (neutral proteinase) and up to 3.9 mmol/l (acid proteinase). In 60 of the 61 women with these values is the activity of proteinases in the second trimester of pregnancy was diagnosed W�eclampsia, and only one of them is the development of pre-eclampsia occurred. Analysis of the obtained values showed that the true-positive result occurred in 60 cases, false - positive in one case, a true negative result was in 34 cases, false negative was absent.
From these data it follows that the accuracy and specificity of the method was 98%.
The above confirms the prognostic value of the claimed method. Thus, the claimed method is more effective in comparison with well-known and has several advantages.
1. High accuracy - 98%, specificity was 98%.
2. Availability of application of the method in clinical practice.
3. A single study.
4. The possibility of early prediction of pre-eclampsia. The claimed method for predicting the development of preeclampsia in the second trimester makes timely preventive therapy of this obstetric pathology that eventually will have favourable impact on perinatal outcomes and reduce neonatal morbidity.
Method for prediction of preeclampsia in the second trimester of pregnancy by examining blood, characterized in that in the serum of women in 7-8 weeks of pregnancy determine the activity of acid and neutral proteinases and acid activity value PR�Teens above 5.6 mmol/l and the activity of neutral proteinases above to 3.9 mmol/l predict the development of preeclampsia in the second trimester of pregnancy.
SUBSTANCE: invention relates to medicine, namely to a method of automated morphometric myelofibrosis diagnostics. The method essence consists in the fact that overview images of zones with different optical properties with determinable fibrous and heamopoietic properties of a biological tissue are performed. Ratios of areas of the said zones of at least three paraffin cuts of trepanobiopsy samples are calculated. The coefficient (Cop) is calculated as the ratio of the fibrous tissue area to the area of the heamopoietic tissue by formula. If the value Cop ≥14.5%, myelofibrosis is diagnosed.
EFFECT: application of the claimed method makes it possible to increase the accuracy and improve the efficiency of myelofibrosis diagnostics.
7 cl, 1 tbl, 4 dwg, 1 ex
SUBSTANCE: testicular germ cells are measured quantitatively. That is ensured by 50-day oral administration of selexen and ascorbic acid into male white rats in doses 1.5 and 500 mg/kg of animal's body weight respectively once a day. 14 days later, administering the selenium-containing biocomplex is accompanied by the 30-minute daily exposure to microwave radiation at 42 GHz (λ=7.1 mm) for 30 days. Once the experimental exposures are completed, the corrective properties of the biocomplex as having an effect on the morphofunctional state of epididymal sperm cells are assessing by formula: MFSI=A+B, wherein MFSI is a morphofunctional state index, A is a portion of normal sperm cells in relation to the reference, and B is a portion of moving sperm cells in relation to the reference. If the MFSI value is 1.3 or more, the spermatogenesis correction is considered to be ineffective, while the MFSI value being more than 1.3 shows the effective spermatogenesis correction if exposed to microwave radiation.
EFFECT: invention enables assessing the spermatogenesis correction efficacy with underlying administration of the biocorrector.
2 tbl, 3 dwg, 3 ex
SUBSTANCE: invention relates to field of medicine, in particular hepatology. Method of quantitative estimation of histological activity in liver biopsy materials in case of chronic diffuse liver diseases results in protocol of counting in absolute numbers, percent and points of pathological signs, identified in the course of morphological analysis of liver biopsy materials from patients with chronic diffuse liver diseases, by sections: necrosis of hepatocytes, globular and atomised fatty hydropic degeneration of hepatocytes, small cell dysplasia of hepatocytes, portal, periportal, intralobular and central infiltration. After that conversion of absolute values into point system is performed by means of said protocol, and histological activity is determined by number of points.
EFFECT: method makes it possible to create quantitative unification of obtained results in case of said diseases.
FIELD: veterinary science.
SUBSTANCE: method includes detection of leukocytes, protein, haemoglobin in faeces, and PH-reaction of faeces. A sample of faeces is dissolved in distilled water and applied drop by drop onto appropriate test fields of a test strip designed for urine testing. By variation of colour within 1 minute the reaction is assumed as positive. If pH is less than 7.0 or more than 7.5, and faeces contain soluble protein, haemoglobin and leukocytes at the same time, availability of the inflammatory process in the intestines is confirmed.
EFFECT: invention makes it possible to quickly and accurately diagnose inflammatory process in intestines.
2 cl, 2 ex
SUBSTANCE: invention relates to medicine and is intended for the treatment of patients with the perforation of gastric and duodenal ulcer. A laparotomy is performed, operative aid concerning the ulcer perforation is realised, during the operation an exudate is sampled from the abdominal cavity, pH of the exudate is determined. If Ph of the said material is 6.6 and lower, a conclusion about the presence in it of anaerobic flora is made and serves the basis for the administration of an antibacterial preparation, influencing the anaerobic flora, into the scheme of initial antibacterial therapy.
EFFECT: method makes it possible to administer specific antibacterial therapy without waiting for culture results.
SUBSTANCE: method involves the integrated clinical-laboratory and microbiological studies of patient's urogenital discharge to determine an agent's cytomorphotype as shown by culture analysis and evaluating a degree of manifestation of the patient's urogenital inflammation or leucocytosis.
EFFECT: invention enables improving the quality of laboratory diagnostics, provides establishing the accurate diagnosis that improves the clinical effectiveness, and reduces the rate of complication accompanying chronic trychomonad infection.
1 tbl, 2 ex
SUBSTANCE: invention refers to medicine, namely to a method for producing cells and non-cell elements of an atherosclerotic plaque. The substance of the method consists in the fact that a balloon angioplasty is followed by washing the cells and non-cell elements; the produced suspension of the cells and non-cell elements is centrifuged; a supernatant fluid is removed; the cells and non-cell elements are re-suspended; the suspensions of the cells and non-cell elements are placed into charged cytobuckets, which are centrifuged; the produced preparations are used for further analysis.
EFFECT: using the declared method provides producing the patient's cells and non-cell elements of the atherosclerotic plaque after the balloon angioplasty for the main indication.
5 cl, 10 dwg, 2 ex
SUBSTANCE: invention represents a method for predicting thromboembolic complications accompanying surgical management in the patients suffering oncologic pulmonary diseases; the method is characterised by the fact that performing a pulmonary surgery involves ligaturing vessels of an involved lung is preceded by approaching its afferent and efferent vessels followed by blood sampling from each vessel and venous blood sampling from a peripheral vein; after the surgery, free heparin is measured in each sample. Thereafter, the parameter K is calculated as the arithmetic mean free heparin content in the afferent vessel of the involved lung and in the venous blood from the peripheral vein; the calculated parameter K is compared to the quantitative free heparin content P; if K is less than P by 25% or less, a high risk of the postoperative thromboembolic complications is predicted, whereas K being less than P by more than 25%, a low risk of the postoperative thromboembolic complications is predicted.
EFFECT: higher accuracy and objectivity of the early postoperative results.
SUBSTANCE: invention represents a method of differential diagnostics of chronic viral hepatitis and fatty liver disease in patients with dyslipidemia syndrome, consisting in the following: activity of the glutathione reductase antioxidant system enzyme (GLR), which is estimated by a spectrophotometric method, is determined in the patient's blood serum, and if GLR value is lower or equals 14.6 mcmol/l/min, the fatty liver disease is diagnosed, if GLR concentration is higher than 14.6 mcmol/l/min, chronic viral hepatitis is diagnosed.
EFFECT: reduction of trauma, increase of availability and simplicity of realisation with high sensitivity, specificity and effectiveness.
4 ex, 1 tbl
SUBSTANCE: substance of the method consists in analysing blood serum by marginal dehydration in polarised light. A mild degree of bronchial asthma is diagnosed if observing anisotropic morphotypes in the form of spherolite crystal aggregations with an integrated globular cluster of weakly anisotropic granules. A moderate degree of bronchial asthma is diagnosed if observing anisotropic morphotypes in the form of spherolite crystals surrounded by incoherent aggregations of weakly anisotropic granules. Diagnosing a severe degree of bronchial asthma is ensured by observing anisotropic morphotypes arranged in various sites of an analytic cell and structured as thin parallel lines, as well as anisotropic morphotypes in the form of spherolite crystals surrounded by incoherent aggregations of weakly anisotropic granules.
EFFECT: method is objective, accurate and accessible; it enables assessing the severity of bronchial asthma by detecting specific anisotropic morphotypes of blood serum.
3 ex, 3 dwg
FIELD: medicine, psychiatry.
SUBSTANCE: one should isolate DNA out of lymphocytes of peripheral venous blood, then due to the method of polymerase chain reaction of DNA synthesis one should amplify the fragments of hSERT locus of serotonin carrier gene and at detecting genotype 12/10 one should predict the risk for the development of hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis.
EFFECT: more objective prediction of disease development.
FIELD: medicine, urology.
SUBSTANCE: one should conduct subcutaneous prevocational tuberculin test and, additionally, both before the test and 48 h later it is necessary to perform the mapping of prostatic vessels and at decreased values of hemodynamics one should diagnose tuberculosis. The information obtained should be documented due to printing dopplerograms.
EFFECT: more reliable and objective information.
1 ex, 1 tbl
FIELD: molecular biology.
SUBSTANCE: the suggested innovation deals with the fact that nucleic acids should be isolated directly out of the sample without pipetting stage but with the help of interconnected reservoirs being prepared beforehand. The above-mentioned vessels should be applied either separately or being interconnected according to standard microtitrating format. The sample should be mixed with a lyzing buffer and nucleic acids are bound with matrix in closed system including, at least, two interconnected reservoirs. Forced movement of sample's mixture and buffer back and forth from one reservoir into another one for several times through narrow passage provides their thorough intermixing. The method provides quick and safe isolation of nucleic acids.
EFFECT: higher efficiency.
44 cl, 4 dwg, 1 ex
FIELD: medicine, phthisiology, microbiology.
SUBSTANCE: diagnostic material is poured preliminary with chlorohexidine bigluconium solution, homogenized, kept at room temperature for 10-12 h and centrifuged. Precipitate is poured with Shkolnikova's liquid medium, incubated at 37oC for 3 days, supernatant part of Shkolnokova's medium is removed, fresh Shkolnikova's medium is added, and precipitate is stirred and inoculated on the dense cellular egg media. Sensitivity of the strain is determined in 3 weeks by the presence of growth in the control tube only. Invention provides enhancing precision and reducing time for assay. Invention can be used in assay for medicinal sensitivity of tuberculosis mycobacterium.
EFFECT: improved assay method.
FIELD: medicine, biotechnology, pharmacy.
SUBSTANCE: invention relates to agents used for treatment of pathological states associated with disorder of synthesis of neuromediating substances. Method involves the development of pharmaceutical composition and a method for it preparing. Pharmaceutical composition represents subcellular synaptosomal fractions: synaptic membranes, "light" synaptosomes and "heavy" synaptosomes prepared from gray matter of cerebral hemispheres from experimental animals based on the goal-seeking modification of humoral mediators of nerve endings transformed to synaptosomes in development and regression of malignant processes. The composition provides inhibiting the growth of tumor cells, to elevate span-life of patients with ascite Ehrlich's sarcoma, breast adenocarcinoma Ca-755, Wolker's carcinosarcoma-256.
EFFECT: valuable medicinal and anti-tumor properties of composition.
12 cl, 3 tbl, 3 ex
SUBSTANCE: method involves carrying out microscopic examination of blood serum samples taken from femoral vein and cubital vein. Femoral vein sample is taken on injured side. The examination is carried out before and after treatment. The blood serum samples are placed on fat-free glass slide in the amount of 0.01-0.02 ml as drops, dried at 18-30°C for 18-24 h. The set of pathological symptoms becoming larger or not changed after the treatment in comparison to sample taken before treatment, and morphological picture of samples under comparison taken from the cubital vein showing no changes or being changed to worse, the treatment is considered to be effective.
EFFECT: enabled medicamentous treatment evaluation in course of treatment to allow the treatment mode to be changed in due time; avoided surgical intervention (amputation); retained active life-style of aged patients.
FIELD: medicine, clinical toxicology.
SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
EFFECT: higher accuracy of prediction.
2 ex, 3 tbl
FIELD: medicine, juvenile clinical nephrology.
SUBSTANCE: disease duration in case of obstructive pyelonephritis should be detected by two ways: either by detecting the value of NADPH-diaphorase activity, as the marker of nitroxide synthase activity in different renal department and comparing it to established norm, or by detecting clinico-laboratory values, such as: hemoglobin, leukocytes, eosinophils, urea, beta-lipoproteides, lymphocytes, neutrophils, the level of glomerular filtration, that of canalicular reabsorption, urinary specific weight, daily excretion of oxalates, arterial pressure, and estimating their deviation against average statistical values by taking into account a child's age.
EFFECT: higher efficiency of detection.
7 dwg, 1 ex, 6 tbl
FIELD: medicine, urology.
SUBSTANCE: the present innovation deals with differential diagnostics of prostatic cancer and other prostatic diseases at the stage of primary inspection. The method includes the detection of PCA and calculation of probability coefficient for prostatic cancer (PCC) by the following formula: where e - the foundation of natural logarithm (e=2.718…), PCA - the level of total blood PCA in ng/ml, V - patient's age in years. At PCC value being above 0.2 one should diagnose prostatic cancer and to establish final diagnosis one should perform polyfocal prostatic biopsy. The method enables to increase accuracy of diagnostics at decreased number of unjustified prostatic biopsies.
EFFECT: higher efficiency of diagnostics.
FIELD: medicine, biology.
SUBSTANCE: invention relates to nutrient medium used for accumulation of cells for the following cytological and/or immunocytochemical analysis carrying out. Invention relates to medium containing salts NaCl, KCl, anhydrous CaCl2, MgSO4 x 6 H2O, MgCl2 x 6 H2O, Na2HPO4 x 2 H2O, KHPO4, NaHCO3, and also glucose and Henx's solution, 10% albumin solution and polyglucin taken in the ratio 1:1:1. Invention provides enhancing the preservation of cells.
EFFECT: improved an valuable properties of nutrient medium.