Method of automated morphometric myelofibrosis diagnostics
SUBSTANCE: invention relates to medicine, namely to a method of automated morphometric myelofibrosis diagnostics. The method essence consists in the fact that overview images of zones with different optical properties with determinable fibrous and heamopoietic properties of a biological tissue are performed. Ratios of areas of the said zones of at least three paraffin cuts of trepanobiopsy samples are calculated. The coefficient (Cop) is calculated as the ratio of the fibrous tissue area to the area of the heamopoietic tissue by formula. If the value Cop ≥14.5%, myelofibrosis is diagnosed.
EFFECT: application of the claimed method makes it possible to increase the accuracy and improve the efficiency of myelofibrosis diagnostics.
7 cl, 1 tbl, 4 dwg, 1 ex
The invention relates to medicine, in particular to Hematology, namely to clinical research methods using automated morphometric methods (including the use of computers), and can be used to diagnose lesions of fibrous tissue, in particular bone marrow tissue (myelofibrosis).
In recent years, thanks to the development and use in clinical practice of new drugs, transplantation of hematopoietic stem cells and many other modern methods of treatment, significantly increased overall survival of patients with neoplastic diseases, in particular hematopoietic tissue. It is obvious that with the increase in life expectancy of cancer patients is significantly increased role of reliable qualitative diagnosis of malignant tumors.
Known conventional methods for diagnosis of myelofibrosis with the help of bone marrow biopsy bone marrow. The scale adopted in the European consensus in 2005 (Thiele J., Kvasnicka Η.Μ, F. Facchetti et al. European consensus for grading of bone marrow fibrosis and assessment of cellularity // Haematilogica. - 2005. - 90:1128 - 1132), allows to carry out qualitative and semi-quantitative assessment of myelofibrosis. Qualitative assessment of myelofibrosis lies in the differentiation between reticulin and collagen, semiquantitative assessment involved determining tightly�ti retikulinovye fiber.
Also known method for automated quantitative assessment of myelofibrosis in myeloproliferative disorders, which consists in determining the average percentage of black pixels in the 3 hematopoietic squares of 0.5 mm2(Teman J. arolin, 2010).
However, the known methods of diagnosis of myelofibrosis do not provide sufficient accuracy and rapidity in obtaining the relevant results.
Also known a method of diagnosing an inflammatory pathology of the maxillary sinuses (RF patent for the invention №2234859 publication of 27.04.2004), which in particular includes the performance review of the radiographs of the paranasal sinuses in neopatriomonal projection, characterized in that the x-ray emit a "region of interest" corresponding to the x-ray contours of the maxillary sinus and the namesake of the orbit, build a histogram and calculate the density ratio (KP) as the ratio of the density of the maxillary sinus, which is expressed in units of gray scale, and density of same orbit, expressed in units of grayscale colors moreover, "one-humped" curve area of interest is the x-ray contour of the entire maxillary sinus, and in the presence of "mnogogolos curve areas of interest are the areas of the sinuses with different densities.
The known method, due and�use, in particular, x-ray technicians, also does not have a high sufficient accuracy and efficiency of diagnosis.
Closest to the claimed invention identified the solution known from the publication (Domnikov N. P. . Structural analysis of trypanosomiases in aggressive and indolent Nahodkinskij lymphomas // Fundamental research, No. 9, 2001, pp. 27-61 (page 58 column 1 and 2), which, in particular, is characterized in that it is determined by the relative area of tumor tissue in different types lympany infiltration of bone marrow by aggressive and indolent non-Hodgkin's lymphoma, which, in any case, facilitates the diagnosis of myelofibrosis.
The main disadvantage of the known method, taken as a prototype, is the lack of opportunities automated morphometric diagnosis of myelofibrosis that hinder improving the accuracy of setting the appropriate diagnosis.
The purpose of the claimed invention is to eliminate the above disadvantages and achieve these technical results, increase accuracy and improve efficiency of diagnosis of myelofibrosis, primary myelofibrosis (chronic idiopathic myelofibrosis), and secondary myelofibrosis that develops when: 1) hematologic diseases (polycythemia Vera, �ssentially thrombocythemia, chronic myelogenous leukemia, hairy cell leukemia, rarely acute leukemia, myelodysplastic syndrome, non-Hodgkin lymphoma, Hodgkin lymphoma, multiple myeloma); 2) solid tumors with metastases in the bone marrow (cancer of breast, lung, prostate); 3) diffuse diseases of connective tissue (systemic lupus erythematosus, systemic sclerosis).
This object is achieved in that the method automated morphometric diagnosis of myelofibrosis, including the execution of overviews of biological tissue, the allocation of such image areas with different optical properties, defined fibrous and hematopoietic properties of biological tissue, and calculating the relationship of these zones, characterized by including the fact that the review image is accomplished by the formation of at least three paraffin sections of trypanosomatid obtained sections were treated as follows: first impregnert silver by the method Gomory, the second stained by the method of van Gizon and third were stained with hematoxylin and eosin, thus obtained observation image is subjected to fixation and analysis so that as a result of this analysis get the value of the areas, colored in black and pink color, corresponding to fibrous tissue, and the magnitude of p�Asadi areas painted in blue and pale pink color corresponding to hematopoietic tissue; then calculate the ratio of the relative areas as a ratio of the area of fibrous tissue to the area of hematopoietic tissue by the formula:
Kop- the ratio of the relative area ( % );
Sblack- an area of fabric, painted black;
Sroses- an area of tissue, colored in pink;
SBL-rose- an area of fabric, painted in pale pink color;
Ssin- an area of tissue, colored blue,
moreover, if set Toop≥14,5% are diagnosed with myelofibrosis.
Method automated morphometric diagnosis of myelofibrosis can be characterized by the fact that paraffin sections of trypanosomatid formed in a thickness of 4 microns.
Method automated morphometric diagnosis of myelofibrosis can be characterized by fixation of overviews of paraffin sections by using a color fotovideostore.
Method automated morphometric diagnosis of myelofibrosis can be characterized by the fact that the area sizes of the areas, colored in black and pink color, corresponding to fibrous tissue, and the area sizes of the areas, colored in blue and pale pink, respectively�adequate hematopoietic tissue, get with the light source and the corresponding RGB sensor colour definition, so that the size of the colored areas correspond to the electrical voltage output from the color sensors.
Method automated morphometric diagnosis of myelofibrosis can be characterized by the size of the painted areas get installed in the circuit after the RGB sensors of the digital signal processor.
Method automated morphometric diagnosis of myelofibrosis can be characterized by the analysis to obtain the values of the squares is colored areas produced using a computerized microscope.
Method automated morphometric diagnosis of myelofibrosis can be characterized by the fact that as a light source using RGB led light source.
In some cases the task is, for example, is solved by the fact that the identification of these indicators of myelofibrosis is carried out using image analysis AxioVision 4.6, using, for example, color packageexists photo/video camera AxioCam, Zeiss microscope and a standard PC. In paraffin sections of trypanosomatid, for example, the Ilium, the thickness of 4 microns, impregnated with silver by the method Gomory and stained by the method of van Gisone, increasing 200 absolute measure�Yu square fibrous (collagen and retikulinovye) tissue and hematopoietic tissue. Studied all the medullary cavity of one of the slice that corresponds to 5-7 fields of view of the microscope and 20-30 fields of view of the camera (depending on the size of the slice). The area of one field of view of the camera is known and 364000 μm2(0.7 mm2). Folded area of fibrous tissue in all fields of view of the camera, get the total absolute area of fibrous tissue within the same slice. Similarly get the total absolute area of hematopoietic tissue. Then calculate the percent area of fibrous tissue from hematopoietic tissue (Kop). Kopreflects the severity of myelofibrosis.
Often the task of diagnostics is empowerment evaluation of myelofibrosis for the purpose of determining such a very meaningful measure, as inhibition of erythroid sprout. The state of erythroid Rostock is evaluated in sections, stained with hematoxylin and eosin.
The proposed method is investigated, for example, trainability Ilium 30 patients with diffuse large b-cell lymphoma (DLBL): 20 men, 10 women, 21 to 59 years, mean age 50,36±14,31 years, 30 patients with chronic lymphocytic leukemia (CLL) and non-Hodgkin's lymphomas of small lymphocytes with neoplastic bone marrow lesion (NHL of small lymphocytes): 18 men, 12 women, from 44 to 78 years, average age - to 59.13±9.5 years, 10 patients with multiple myeloma (MM): 5 males, 5 females aged 50 to 68 years, mean age 59,67±6,58 years who were treated in GBUZ NSO "Novosibirsk State regional clinical hospital from 2010 to 2012.
The diagnosis of lymphoma was established in accordance with the REAL classification (1994) and who (2008), the diagnosis of chronic lymphocytic leukemia was made on the basis of the classification Binet (Binet J. L. et. al., 1981). To verify the diagnosis of multiple myeloma criteria used Y. Huang et al. (Y. Huang W. et al.,1 999).
The first group consisted of sections 14 trypanosomatid noted, most likely, only retikulinovye myelofibrosis, in the second group sections 13 trypanosomatid, where there was probably only collagen myelofibrosis, in the third - slices 15 trypanosomatid in which with the greatest certainty was identified as collagen and retikulinovye myelofibrosis. In the remaining 28 trypanosomatid - fibrous tissue is not detected. Assessment of severity of myelofibrosis was carried out in each group.
The Table presents example indicators of myelofibrosis associated with erythroid hypoplasia of Rostock, in particular the data of the results of automated morphometric evaluation of myelofibrosis with lymphoproliferative diseases identified indicators associated with inhibition of eritr�LiDE Rostock.
These tables allow to conclude that these indicators include: in the first group - Kop≥36.7 per cent, in the second group - Kop≥22.5 percent, in the third group To theop- ≥14.5 percent. Thus, the implementation of the method, in the part of these values, such asop>14.5%, and can be used in the diagnosis of myelofibrosis, without a clear separation into groups.
Fig. 1 schematically shows an example of an installation for analysis of paraffin sections trainability claimed method. Fig. 2, Fig. 3 and Fig. 4 shows an enlarged 200 times photographic prints paraffin sections trainability Ilium, subject to such analysis for the purpose of diagnosing the lesion tissue myelofibrosis. In the presented figures the numbers indicate the following:
1. The source of light.
2. Photographic print drug cutoff trainability.
3. Frame photographic print.
4. The direction of light transmitted through the photo of the drug.
5. The sensor matrix colour definition.
6. The connectors.
7. A digital signal processor.
9. Zone, black in color.
10. Zone pink color.
11. Area bright pink color.
12. Area pale pink color.
13. Area blue color.
In the presented example, the value of the squares of the desired zones in paraffin sections trainability defined�s as follows. Obtained in the standard way, three paraffin slice trainability, for example, the Ilium, the thickness of 4 μm, sequentially processed as follows: first impregnert silver by the method Gomory, the second stained by the method of van Gizon and third were stained with hematoxylin and eosin. Thus obtained observation image photographed, thereby fixing the obtained color-coded zones.
With the help of light source 1, for example in the form of a matrix of LEDs, a beam of white light is directed to a photographic picture of the drug cut trainability 2 mounted in a frame 3. Further, passing through a photographic picture of the drug cut trainability 2, the light is colored in colors corresponding to the colors selected for the analysis of photographic print of the drug cutoff trainability 2 in direction of 4, and gets on RGB matrix electronic sensors color definition 5 (e.g., digital). The output matrix of electronic sensors color definition 5 is formed voltage (Ur, Ug, Ub), is proportional to the flow of light of the respective colors. Using connectors 6 outputs matrix electronic sensors determine the color 5 pieces, in particular a digital signal processor, which identifies the area of the painted areas analyzed photographic print of the drug cut the tre�adobeupdate, on the basis of proportionality from the output voltages of the respective colors (URUGUB), the results of the determination of the required areas is read from the indicator 8, for example, in the form of monitor.
All three of the analyzed slice is separated from the test sample of the Ilium in a row one after the other, and considering that the slice thickness does not exceed a few microns, they can be found almost identical drugs. The first drug in paraffin slice trainability (Fig. 2) impregnert silver, with black painted retikulinovye fabric (black color 9). A second drug paraffin slice trainability (Fig. 3) stained by the method of van Gisone, collagen tissue becomes pink zone pink color 10). The third drug paraffin slice trainability (Fig. 4) was treated with hematoxylin and eosin, wherein the hematopoietic tissue is colored in pale pink (cytoplasm) (zone pale pink color 12) and in blue (cell nucleus) (zone blue color 13). Bone preparations, stained by van gieson and hematoxylin-eosin, has a bright pink shade (zone of bright pink fabric 11), the area of fibrous tissue is determined by the sum of the areas of the zones of the black color zones 9 and pink color 10. The area of hematopoietic tissue is determined by summo� areas blue zones 12 and pale pink 13 colors.
Further, to obtain the desired final diagnosis of lesions of the studied sample myelofibrosis compute the ratio of the relative area ( % ) are presented in the claimed invention formula. Suppose that in the proposed example, Kopcalculated for slices trainability, which noted only retikulinovye myelofibrosis, equal to 37%, then according to the above regulations, this indicator is important for the inhibition of erythroid sprout. In sections of trypanosomatid only with collagen myelofibrosis important for inhibition of erythroid city is home To theopfor example , 23%. In slices with retikulinovye and collagen myelofibrosis important to the oppression of Central erythron component is, for example, Kop15%. Thus, when getting To theop≥14,5% in any case clearly diagnosed myelofibrosis, even if no unambiguous dividing it into groups.
The high accuracy of the proposed method is achieved by using the automated estimation of small quantities of the variation in fibrous areas and hematopoietic tissues, and the efficiency is improved due to the use of automated (computerized) method of determining the ratio of said areas.
Thus, the inventive method is based on automatic�teachers morphometric study allows for non-Hodgkin's lymphoma, chronic lymphocytic leukemia, multiple myeloma and other diseases to maintain accurate and rapid diagnosis of myelofibrosis.
The inventive method is industrially applicable, as it does not require any specific complex equipment, in addition to the standard set of medical and computer equipment that makes it possible to widely implement it in clinics, research and educational institutions.
1. Method automated morphometric diagnosis of myelofibrosis, including the execution of overviews of biological tissue, the allocation of such image areas with different optical properties, defined fibrous and hematopoietic properties of biological tissue, and calculating the relationship of these zones, characterized in that the observation image is performed by forming at least three paraffin sections of trypanosomatid obtained sections were treated as follows: first impregnert silver by the method Gomory, the second stained by the method of van Gizon and third were stained with hematoxylin and eosin, thus obtained observation image is subjected to fixation and analysis, the result of this analysis get the value of p�Asadi areas
painted in black and pink colors, corresponding to fibrous tissue, and the area sizes of the areas, colored in blue and pale pink color corresponding to hematopoietic tissue; then calculate the ratio of the relative areas as a ratio of the area of fibrous tissue to the area of hematopoietic tissue by the formula:
Kop- the ratio of the relative area ( % );
Sblack- an area of fabric, painted black;
Sroses- an area of tissue, colored in pink;
SBL-rose- an area of fabric, painted in pale pink color;
Ssin- an area of tissue, colored blue,
moreover, if set Toop≥14,5% are diagnosed with myelofibrosis.
2. Method automated morphometric diagnosis of myelofibrosis according to claim 1, characterized in that the paraffin sections of trypanosomatid formed in a thickness of 4 microns.
3. Method automated morphometric diagnosis of myelofibrosis according to claim 1, characterized in that the fixation overviews of paraffin sections by using a color fotovideostore.
4. Method automated morphometric diagnosis of myelofibrosis according to claim 1, characterized in that the area sizes of the areas, colored in black and pink color, corresponding to fibrous tissue, and in�mask areas, painted in blue and pale pink color corresponding to hematopoietic tissue, are produced by the light source and the corresponding RGB sensor colour definition, so that the size of the colored areas correspond to the electrical voltage output from the color sensors.
5. Method automated morphometric diagnosis of myelofibrosis according to claim 4, characterized in that the area of the painted areas get installed in the circuit after the RGB sensors of the digital signal processor.
6. Method automated morphometric diagnosis of myelofibrosis according to claim 4, characterized in that the analysis to obtain the values of the squares is colored areas produced using a computerized microscope.
7. Method automated morphometric diagnosis of myelofibrosis according to claim 4, characterized in that the light source using RGB led light source.
SUBSTANCE: testicular germ cells are measured quantitatively. That is ensured by 50-day oral administration of selexen and ascorbic acid into male white rats in doses 1.5 and 500 mg/kg of animal's body weight respectively once a day. 14 days later, administering the selenium-containing biocomplex is accompanied by the 30-minute daily exposure to microwave radiation at 42 GHz (λ=7.1 mm) for 30 days. Once the experimental exposures are completed, the corrective properties of the biocomplex as having an effect on the morphofunctional state of epididymal sperm cells are assessing by formula: MFSI=A+B, wherein MFSI is a morphofunctional state index, A is a portion of normal sperm cells in relation to the reference, and B is a portion of moving sperm cells in relation to the reference. If the MFSI value is 1.3 or more, the spermatogenesis correction is considered to be ineffective, while the MFSI value being more than 1.3 shows the effective spermatogenesis correction if exposed to microwave radiation.
EFFECT: invention enables assessing the spermatogenesis correction efficacy with underlying administration of the biocorrector.
2 tbl, 3 dwg, 3 ex
SUBSTANCE: invention relates to field of medicine, in particular hepatology. Method of quantitative estimation of histological activity in liver biopsy materials in case of chronic diffuse liver diseases results in protocol of counting in absolute numbers, percent and points of pathological signs, identified in the course of morphological analysis of liver biopsy materials from patients with chronic diffuse liver diseases, by sections: necrosis of hepatocytes, globular and atomised fatty hydropic degeneration of hepatocytes, small cell dysplasia of hepatocytes, portal, periportal, intralobular and central infiltration. After that conversion of absolute values into point system is performed by means of said protocol, and histological activity is determined by number of points.
EFFECT: method makes it possible to create quantitative unification of obtained results in case of said diseases.
FIELD: veterinary science.
SUBSTANCE: method includes detection of leukocytes, protein, haemoglobin in faeces, and PH-reaction of faeces. A sample of faeces is dissolved in distilled water and applied drop by drop onto appropriate test fields of a test strip designed for urine testing. By variation of colour within 1 minute the reaction is assumed as positive. If pH is less than 7.0 or more than 7.5, and faeces contain soluble protein, haemoglobin and leukocytes at the same time, availability of the inflammatory process in the intestines is confirmed.
EFFECT: invention makes it possible to quickly and accurately diagnose inflammatory process in intestines.
2 cl, 2 ex
SUBSTANCE: invention relates to medicine and is intended for the treatment of patients with the perforation of gastric and duodenal ulcer. A laparotomy is performed, operative aid concerning the ulcer perforation is realised, during the operation an exudate is sampled from the abdominal cavity, pH of the exudate is determined. If Ph of the said material is 6.6 and lower, a conclusion about the presence in it of anaerobic flora is made and serves the basis for the administration of an antibacterial preparation, influencing the anaerobic flora, into the scheme of initial antibacterial therapy.
EFFECT: method makes it possible to administer specific antibacterial therapy without waiting for culture results.
SUBSTANCE: method involves the integrated clinical-laboratory and microbiological studies of patient's urogenital discharge to determine an agent's cytomorphotype as shown by culture analysis and evaluating a degree of manifestation of the patient's urogenital inflammation or leucocytosis.
EFFECT: invention enables improving the quality of laboratory diagnostics, provides establishing the accurate diagnosis that improves the clinical effectiveness, and reduces the rate of complication accompanying chronic trychomonad infection.
1 tbl, 2 ex
SUBSTANCE: invention refers to medicine, namely to a method for producing cells and non-cell elements of an atherosclerotic plaque. The substance of the method consists in the fact that a balloon angioplasty is followed by washing the cells and non-cell elements; the produced suspension of the cells and non-cell elements is centrifuged; a supernatant fluid is removed; the cells and non-cell elements are re-suspended; the suspensions of the cells and non-cell elements are placed into charged cytobuckets, which are centrifuged; the produced preparations are used for further analysis.
EFFECT: using the declared method provides producing the patient's cells and non-cell elements of the atherosclerotic plaque after the balloon angioplasty for the main indication.
5 cl, 10 dwg, 2 ex
SUBSTANCE: invention represents a method for predicting thromboembolic complications accompanying surgical management in the patients suffering oncologic pulmonary diseases; the method is characterised by the fact that performing a pulmonary surgery involves ligaturing vessels of an involved lung is preceded by approaching its afferent and efferent vessels followed by blood sampling from each vessel and venous blood sampling from a peripheral vein; after the surgery, free heparin is measured in each sample. Thereafter, the parameter K is calculated as the arithmetic mean free heparin content in the afferent vessel of the involved lung and in the venous blood from the peripheral vein; the calculated parameter K is compared to the quantitative free heparin content P; if K is less than P by 25% or less, a high risk of the postoperative thromboembolic complications is predicted, whereas K being less than P by more than 25%, a low risk of the postoperative thromboembolic complications is predicted.
EFFECT: higher accuracy and objectivity of the early postoperative results.
SUBSTANCE: invention represents a method of differential diagnostics of chronic viral hepatitis and fatty liver disease in patients with dyslipidemia syndrome, consisting in the following: activity of the glutathione reductase antioxidant system enzyme (GLR), which is estimated by a spectrophotometric method, is determined in the patient's blood serum, and if GLR value is lower or equals 14.6 mcmol/l/min, the fatty liver disease is diagnosed, if GLR concentration is higher than 14.6 mcmol/l/min, chronic viral hepatitis is diagnosed.
EFFECT: reduction of trauma, increase of availability and simplicity of realisation with high sensitivity, specificity and effectiveness.
4 ex, 1 tbl
SUBSTANCE: substance of the method consists in analysing blood serum by marginal dehydration in polarised light. A mild degree of bronchial asthma is diagnosed if observing anisotropic morphotypes in the form of spherolite crystal aggregations with an integrated globular cluster of weakly anisotropic granules. A moderate degree of bronchial asthma is diagnosed if observing anisotropic morphotypes in the form of spherolite crystals surrounded by incoherent aggregations of weakly anisotropic granules. Diagnosing a severe degree of bronchial asthma is ensured by observing anisotropic morphotypes arranged in various sites of an analytic cell and structured as thin parallel lines, as well as anisotropic morphotypes in the form of spherolite crystals surrounded by incoherent aggregations of weakly anisotropic granules.
EFFECT: method is objective, accurate and accessible; it enables assessing the severity of bronchial asthma by detecting specific anisotropic morphotypes of blood serum.
3 ex, 3 dwg
SUBSTANCE: CEA, CA 19-9, CA 72-4, M2-PK cancer-specific markers and Ki-67 proliferation index are pre-determined; combined positron emission and computer tomography (PET/CT) are performed. Up to two procedures of neoadjuvant intra-arterial chemoembolisation are conducted with the first one performed by administering a half single systemic dose of irinotecan in lipiodol 4-6 ml; no sooner than 2-3 weeks later, the cancer-specific markers and Ki-67 proliferation index and PET/CT SUVmax are checked; if at least one of this factors tends to increase as compared to references, the intra-arterial chemoembolisation is conducted again by administering at least 1/3 single systemic dose of irinotecan in no more than lipiodol 5 ml. Not later than 10-14 days following the second chemoembolisation or no sooner than 2-3 weeks after the first chemoembolisation, a surgical intervention is required if the tumour keeps its metabolic and proliferative activity on these days.
EFFECT: method enables decreasing the tumour process activity and performing the surgical intervention at the moment of extreme devitalisation of the tumour and micro-disseminates, and thereby improving the remote therapeutic effects.
2 cl, 4 dwg, 3 ex
FIELD: medicine, psychiatry.
SUBSTANCE: one should isolate DNA out of lymphocytes of peripheral venous blood, then due to the method of polymerase chain reaction of DNA synthesis one should amplify the fragments of hSERT locus of serotonin carrier gene and at detecting genotype 12/10 one should predict the risk for the development of hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis.
EFFECT: more objective prediction of disease development.
FIELD: medicine, urology.
SUBSTANCE: one should conduct subcutaneous prevocational tuberculin test and, additionally, both before the test and 48 h later it is necessary to perform the mapping of prostatic vessels and at decreased values of hemodynamics one should diagnose tuberculosis. The information obtained should be documented due to printing dopplerograms.
EFFECT: more reliable and objective information.
1 ex, 1 tbl
FIELD: molecular biology.
SUBSTANCE: the suggested innovation deals with the fact that nucleic acids should be isolated directly out of the sample without pipetting stage but with the help of interconnected reservoirs being prepared beforehand. The above-mentioned vessels should be applied either separately or being interconnected according to standard microtitrating format. The sample should be mixed with a lyzing buffer and nucleic acids are bound with matrix in closed system including, at least, two interconnected reservoirs. Forced movement of sample's mixture and buffer back and forth from one reservoir into another one for several times through narrow passage provides their thorough intermixing. The method provides quick and safe isolation of nucleic acids.
EFFECT: higher efficiency.
44 cl, 4 dwg, 1 ex
FIELD: medicine, phthisiology, microbiology.
SUBSTANCE: diagnostic material is poured preliminary with chlorohexidine bigluconium solution, homogenized, kept at room temperature for 10-12 h and centrifuged. Precipitate is poured with Shkolnikova's liquid medium, incubated at 37oC for 3 days, supernatant part of Shkolnokova's medium is removed, fresh Shkolnikova's medium is added, and precipitate is stirred and inoculated on the dense cellular egg media. Sensitivity of the strain is determined in 3 weeks by the presence of growth in the control tube only. Invention provides enhancing precision and reducing time for assay. Invention can be used in assay for medicinal sensitivity of tuberculosis mycobacterium.
EFFECT: improved assay method.
FIELD: medicine, biotechnology, pharmacy.
SUBSTANCE: invention relates to agents used for treatment of pathological states associated with disorder of synthesis of neuromediating substances. Method involves the development of pharmaceutical composition and a method for it preparing. Pharmaceutical composition represents subcellular synaptosomal fractions: synaptic membranes, "light" synaptosomes and "heavy" synaptosomes prepared from gray matter of cerebral hemispheres from experimental animals based on the goal-seeking modification of humoral mediators of nerve endings transformed to synaptosomes in development and regression of malignant processes. The composition provides inhibiting the growth of tumor cells, to elevate span-life of patients with ascite Ehrlich's sarcoma, breast adenocarcinoma Ca-755, Wolker's carcinosarcoma-256.
EFFECT: valuable medicinal and anti-tumor properties of composition.
12 cl, 3 tbl, 3 ex
SUBSTANCE: method involves carrying out microscopic examination of blood serum samples taken from femoral vein and cubital vein. Femoral vein sample is taken on injured side. The examination is carried out before and after treatment. The blood serum samples are placed on fat-free glass slide in the amount of 0.01-0.02 ml as drops, dried at 18-30°C for 18-24 h. The set of pathological symptoms becoming larger or not changed after the treatment in comparison to sample taken before treatment, and morphological picture of samples under comparison taken from the cubital vein showing no changes or being changed to worse, the treatment is considered to be effective.
EFFECT: enabled medicamentous treatment evaluation in course of treatment to allow the treatment mode to be changed in due time; avoided surgical intervention (amputation); retained active life-style of aged patients.
FIELD: medicine, clinical toxicology.
SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
EFFECT: higher accuracy of prediction.
2 ex, 3 tbl
FIELD: medicine, juvenile clinical nephrology.
SUBSTANCE: disease duration in case of obstructive pyelonephritis should be detected by two ways: either by detecting the value of NADPH-diaphorase activity, as the marker of nitroxide synthase activity in different renal department and comparing it to established norm, or by detecting clinico-laboratory values, such as: hemoglobin, leukocytes, eosinophils, urea, beta-lipoproteides, lymphocytes, neutrophils, the level of glomerular filtration, that of canalicular reabsorption, urinary specific weight, daily excretion of oxalates, arterial pressure, and estimating their deviation against average statistical values by taking into account a child's age.
EFFECT: higher efficiency of detection.
7 dwg, 1 ex, 6 tbl
FIELD: medicine, urology.
SUBSTANCE: the present innovation deals with differential diagnostics of prostatic cancer and other prostatic diseases at the stage of primary inspection. The method includes the detection of PCA and calculation of probability coefficient for prostatic cancer (PCC) by the following formula: where e - the foundation of natural logarithm (e=2.718…), PCA - the level of total blood PCA in ng/ml, V - patient's age in years. At PCC value being above 0.2 one should diagnose prostatic cancer and to establish final diagnosis one should perform polyfocal prostatic biopsy. The method enables to increase accuracy of diagnostics at decreased number of unjustified prostatic biopsies.
EFFECT: higher efficiency of diagnostics.
FIELD: medicine, biology.
SUBSTANCE: invention relates to nutrient medium used for accumulation of cells for the following cytological and/or immunocytochemical analysis carrying out. Invention relates to medium containing salts NaCl, KCl, anhydrous CaCl2, MgSO4 x 6 H2O, MgCl2 x 6 H2O, Na2HPO4 x 2 H2O, KHPO4, NaHCO3, and also glucose and Henx's solution, 10% albumin solution and polyglucin taken in the ratio 1:1:1. Invention provides enhancing the preservation of cells.
EFFECT: improved an valuable properties of nutrient medium.