Method for assessing spermatogenesis correction efficacy in animals exposed for microwave radiation

FIELD: medicine.

SUBSTANCE: testicular germ cells are measured quantitatively. That is ensured by 50-day oral administration of selexen and ascorbic acid into male white rats in doses 1.5 and 500 mg/kg of animal's body weight respectively once a day. 14 days later, administering the selenium-containing biocomplex is accompanied by the 30-minute daily exposure to microwave radiation at 42 GHz (λ=7.1 mm) for 30 days. Once the experimental exposures are completed, the corrective properties of the biocomplex as having an effect on the morphofunctional state of epididymal sperm cells are assessing by formula: MFSI=A+B, wherein MFSI is a morphofunctional state index, A is a portion of normal sperm cells in relation to the reference, and B is a portion of moving sperm cells in relation to the reference. If the MFSI value is 1.3 or more, the spermatogenesis correction is considered to be ineffective, while the MFSI value being more than 1.3 shows the effective spermatogenesis correction if exposed to microwave radiation.

EFFECT: invention enables assessing the spermatogenesis correction efficacy with underlying administration of the biocorrector.

2 tbl, 3 dwg, 3 ex

 

The invention relates to medicine, namely to the field of molecular physiology and biochemistry and can be used to improve spermatogenesis in animals under conditions of chronic exposure to microwave radiation of extremely high frequencies.

The known method of correction of spermatogenesis, which consists in the use of selenium-containing compounds (sodium Selenite) is added under conditions of chronic intoxication hydrogen sulfide-containing gas Astrakhan gas field (Ushakov M. V. functioning of the reproductive system of male rats during chronic exposure to natural toxicants: abstract dis. ... candidate. Biol. Sciences. - Astrakhan, 2002. - 22 p.). However, the known method has the following disadvantages:

- as protector the author proposes an inorganic form of selenium (sodium Selenite), which, unlike organic, is trudnousvoyaemoe;

- Sam selenium-containing protector is not supplemented by any known vitamin-antioxidant (vitamin E, ascorbic acid), is able to strengthen protective properties of selenium;

- as a stress factor was used chemical toxicant - Astrakhan hydrogen sulfide-containing natural gas.

Known methods of correction of spermatogenesis in animals with combined effects of organic selenium in the form of antioxidants (vitamin E, ascorbic acid) (Echeverria-Alonzo S., Santos-Ricalde, R., Centurion-Castro F., Ake-Lopez R., Alfaro-Gamboa, M., Rodriquez-Buenfll J. Effects of dietary selenium and vitamin E on semen quality and sperm morphology of young boars during warm and fresh season // Journal of Animal and Veterinary Advances. - 2009. - Vol. 8, No. 11. - P. 2311-2317; Loginov V. P., Nikolaev A. A., Vetoshkin P. B. Method of correction of spermatogenesis in animals under conditions of chronic intoxication with natural gas: Pat. 2480221 Grew. Federation. No. 2012107565/15; Appl. 28.02.2012; publ. 27.04.2013, bull. No. 12. - 8 S.). However, the known methods have the following disadvantages:

- as extreme exposure conditions are the following factors: (a) thermal factor (temperature) associated with seasonal rhythm, and b) chemical factor (natural gas);

- no quantitative criterion for the efficiency of spermatogenesis in terms of exposure to adverse factors against the use of Biocorrector.

The method for evaluation of spermatogenesis in the immature sex cells of the ejaculate (Kurilo L. F., Dubinsky V. P. Ostroumova, T., etc. In Assessment of spermatogenesis in immature sex cells of the ejaculate // problems of reproduction. - 1995. - Vol. 1, No. 3. Pp. 33-36). However, the known method has the following disadvantages:

- evaluation of spermatogenesis is given based on the analysis of immature spermatogenic cells, was not involved in the fertilization process;

- no quantitative criterion of the effectiveness of the spermatogenic process in C�scrap.

Also known prototype method quantitative cytological evaluation of the efficiency of spermatogenesis in patent No. 2231979 from 15.06.2001 (publ. 10.07.2004). The prototype has the following disadvantages:

- provides a quantitative assessment of the morphological forms of germ cells testicular tissue in General;

is not taken into account the kinetic characteristics of Mature gametes - spermatozoa;

- not given integrated assessment of the morphology and kinetics of sperm to interpret the effectiveness of the spermatogenic process.

The present invention allows to estimate the efficiency of correction of spermatogenesis in terms of exposure to microwave radiation of extremely high frequencies on the background of the input Biocorrector using the integrated criterion of morphofunctional state indicate epididymal spermatozoa. The proposed method is able to quantify the efficiency of spermatogenesis in different experimental conditions relative to benchmarks.

Said technical result is achieved in that the male albino rats orally administered selexin and ascorbic acid, respectively, at doses of 1.5 and 500 mg/kg animal body weight of 1 times a day for 50 days, and after 14 days on the background of the introduction of selenium-containing biocomplex is exposed to microwave radiation with a frequency of 42 GHz (λ=7.1 m�) within 30 days of 30 minutes daily, then at the end of the scheme of the experimental impact estimate corrective properties of biogas plant in relation to the morphofunctional state indicate epididymal sperm by the formula:

IMPS=A+b, where

IMPS - the index of the morphofunctional state,

A - the proportion of normal spermatozoa compared to the control,

- Fraction of motile sperm relative to control,

and when values of IMFS less than or equal to 1.3 judged ineffective correction of spermatogenesis, while IMPS more than 1.3 judged on effective correction of spermatogenesis in terms of exposure to microwave radiation.

From small rodents microwave radiation even millimeter range causes inhibition of spermatogenic function (M. G. Kuznetsov. The function of the reproductive system of male rats under the influence of electromagnetic radiation of millimeter range. Author. dis. ... candidate. Biol. Sciences. - Astrakhan, 2009. - 24 S.; Nikolaev, A. A., Kuznetsov M. G., Serdyukov V. G. Gonadotoxic action of millimeter radiation: Monograph. - Astrakhan: Publishing house "sbei HPE AGMA", 2013. - 78 p.). This raises the problem of correction of spermatogenesis in terms of exposure to microwave radiation. As a reliable proofreader can act bioelement selenium (A. I. Polunin, D. L. Lutsky, Miroshnikov, V. M. and others Selenium and zinc in the correction of muisc�th subfertility: a manual for physicians. - Astrakhan: AGMA, 2002. - 42 p.). Selenium in organic form has a high degree of bioavailability and low toxicity (Sanok I. V. Irreplaceable selenium. - M.: Association AKM, 2001. - 96 p.). Animals were injected selenium (350 mcg of selenium per 1 kg of animal body weight per day, which is adequate for Mature rats from the point of view of the level of their metabolism (Ushakov M. V. functioning of the reproductive system of male rats during chronic exposure to natural toxicants. The author's abstract dis. ... candidate. Biol. Sciences. - Astrakhan, 2002. - 22 p.). Ascorbic acid was given to animals in moderately high-dose, still relying on old research by L. Pauling and other researchers about the role of high doses of vitamin C in the regulation of reproductive processes (Pauling L. Vitamin C and health. M.: Nauka, 1974. - 74 p.; A. Sharaf, N. Gomaa Androgenicity in vitamins // Qualitas plantarum et material vegetabiles. - 1970. - Vol. 19, No. 4. - P. 369-374; Yousef M. I., Abdallah G. A., Kamel, K. I. Effect of ascorbic acid and vitamin E supplementation on semen quality and biochemical parameters of male rabbits // Anim. Reprod. Sci. - 2003. Vol. 76, No. 1-2. - P. 99-111). The addition of selexin ascorbic acid provides the output excessive amounts of selenium from the body and increases antioxidant status (Bebne D. Studies in the distribution and characteristics of new mammalian selenium-containing proteins // Analyst. - 1995. - Vol. 120. - P. 823-825). Given that the spermatogenic cycle in rats is 48 days (who, 1967), animals were assigned to the 50-day diet enriched in se�axinom and ascorbic acid. 2 weeks after the initiation of selexin and ascorbic acid began to influence microwave radiation with a frequency of 42 GHz. The specified frequency radiation refers to millimeter range of electromagnetic waves (microwave radiation of extremely high frequency EHF) emanating from a number of sources (radars, medical equipment, telecommunication) with whom you are in daily life (GOST 24375-80). The influence of microwave radiation was carried out inside the course of the introduction of selexin and ascorbic acid (from the third week in a period of 30 days), allowing to smoothly introduce this factor of physical stress in an experimental model, thus avoiding errors due to the primary effects of radiation in conjunction with the accompanying manipulations on animals in the absence of correctors (selexin and ascorbic acid). Exposure to radiation was carried out for 30 minutes, which simulates the conditions of real life (the effects of radio communication, the impact of medical devices, magnetic fields generated by radar installations).

Because in the epididymis of the testis occurs the maturation of sperm, the analysis was subjected to suspension indicate epididymal spermatozoa. For the development of integrated assessment criterion Mohr�funktsionalnogo condition of the testes were taken as a basis of two approaches: 1) quantification of morphologically normal forms of the sperm; 2) evaluation of kinetic properties (mobility) indicate epididymal spermatozoa. To create an integrated criterion it is convenient to Express the quantitative indicators for the adopted standard. In the classic sense, that standard may be a control (intact) group. Next, the resulting relative indicators (ratios) are added and the result is a criterion, and taking into account the kinetics, and morphology of cells.

Percentage of normal forms of spermatozoa is calculated by subtracting from 100% the total number of defective and dead sperm percentage. The relative number of normal spermatozoa is calculated by dividing the percentage of normal forms of spermatozoa in the experimental group on the percentage of normal forms in the control by the formula (1):

where A is the proportion of normal sperm cells relative to control, P and PKpercent number of defective sperm, respectively, in the experimental and control groups, D and DK - percentage of dead spermatozoa, respectively in the experimental and control groups.

The ratio of sperm motility will be assessed by calculating the proportion of motile sperm compared to the control by the formula (2):

where b - share stock�tion of sperm cells relative to control, M - mobility indicate epididymal spermatozoa in the experimental group, %, MK- indicate epididymal motility of spermatozoa in the control group, %.

Then the integrated index of morphofunctional state (IMPS) indicate epididymal sperm will take into account the coefficients A and b by the formula (3):

In addition, you can also calculate the average quality of sperm cells (CSC) in percent by dividing in half IMPS and multiplying by 100% by the formula (4):

Here the division of the index IMPS in half means calculating the average value of the sum A+b with subsequent expression of the resulting values in percent.

A method of estimating the correction spermatogenic function was carried out on 40 adult male Wistar rats weighing 210±10 g at the Department of chemistry chair of sbee HPE "Astrakhan state medical Academy" of rmph. The animals were divided into 4 groups. The first group was a control (10 PCs). The second group (10 PCs) included animals treated orally with seleken in combination with ascorbic acid in doses of 1.5 and 500 mg/kg animal body weight per day for 50 days. The third group consisted of animals exposed to microwave radiation of millimeter range (λ=7.1 mm, frequency f=42 G�C) within 30 days for 30 minutes. To create an electromagnetic field using a generator of monochromatic electromagnetic waves JAV-1-7,1 (Russia). In the fourth group consisted of animals treated with seleken in combination with ascorbic acid in the above daily doses for 50 days, and in parallel through 14 days (third week) from the start of administration of these drugs is exposed to microwave radiation with a frequency of 42 GHz for 30 days for 30 minutes a day.

To assess the protective properties of selexin in combination with ascorbic acid in terms of exposure to microwave radiation was investigated ejaculate white rats. To do this, at the end of the experiments from the tail of epididymis, slice them lengthwise, washed semen metered amounts of saline (empirically for rats is the amount of 2-4 ml) (I. V. Sanok, V. N. Fomenko. Long-term effects of the influence of chemical compounds on the body. - M.: Medicine, 1979. - 232 p.) and received a suspension of (indicate epididymal spermatozoa in animals of the third group were isolated on day 7 after exposure to microwave radiation). In a watch glass within 2 minutes of epididymis actively moved for exemption from parts of the sperm (to avoid artifacts glass rod cannot be used, use segments of washed rubber Tr�KJV). A suspension of sperm cells were applied to glass slides, dried in air, fixed over the flame and dyed with any dye. Counting degenerative forms were in relation to the amount calculated in fields of view of the sperm. Counting the total number indicate epididymal spermatozoa in the suspension produced in the camera Goryaeva under the eyepiece of a light microscope at a magnification of 400x. The number of sperm counted in 5 large squares microscope slides diagonally. In addition, in native semen was determined by the percentage of different morphological forms of spermatozoa (defective, movable and dead). Additionally, to confirm the quantitative indicators were given General characteristics of the morphofunctional state of the seminiferous tubules on the basis of histological sections. Sections of the testes of a thickness of 7 μm were produced on a microtome "Microm NM - 400 (Germany). Sections were stained with hematoxylineosin. The obtained preparations were studied on the universal microscope "Nu" (Germany), connected to a color television camera "Pixera" (USA). All the findings of the experiment data is statistically processed using student's criterion (Lakin GF Biometrics: a textbook for Biol. spec. universities. - M.: Higher school, 1990. - 352 p.).

In the proposed method the results were as follows. Koli�the degree of morphological and functional indicators indicate epididymal sperm in white rats in norm and in conditions of experimental effects is provided in table 1. The change in the number indicate epididymal spermatozoa under the influence of microwave radiation selenium-containing biocomplex (selexin + vitamin C) and their combinations is shown in figure 1. In animals exposed to microwave radiation, a marked decrease in the overall number indicate epididymal sperm in 1,5 times in comparison with the control (P<0,05) (table 1, Fig. 1). The number of defective spermatozoa (30,5%) grew 1.7 times in comparison with the control (18,2%). The number of dead sperm increased in 3 times (30.2 per cent) compared with the control (9,8%) (P<0,001). The lack of sperm motility is mainly due to this defect, as the breakup of the tail, which can be explained by the strengthening of the process of lipid peroxidation in terms of exposure to microwave radiation. The consumption of selenium-containing biocomplex caused the improvement of mobility and contributed to the increase in the number indicate epididymal sperm compared with the control group animals. The number of dead spermatozoa was decreased more than 2 times compared to control (P<0,001). Improved kinetic properties and the viability of sperm when receiving specified biocomplex can be explained by the fact that in the tail of spermatozoa of rats contains Selenite, which has important structural importance in the Assembly of the tail spermato�of oidov (M. V. Ushakov The function of the reproductive system of male rats during chronic exposure to natural toxicants. The author's abstract dis. ... candidate. Biol. Sciences. - Astrakhan, 2002. - 22 p.). However prior consumption of selenium-containing animals of biocomplex contributed to the reduction of the destructive effects of microwave radiation. Authentically significant deviations from control sperm count in animals exposed to microwave radiation and pre-treated with the indicated bioprotector, we have not been fixed. Tended to increase in total indicate epididymal spermatozoa. The number of defective spermatozoa in the group of animals exposed to microwave radiation in patients receiving selenium-containing biocomplex, did not differ significantly from that of control group. Selenium-containing biocomplex contributed to some improvement of sperm motility in terms of exposure to microwave radiation compared to the group of animals exposed only to radiation exposure. The number of dead spermatozoa was markedly lower in terms of exposure to microwave radiation in patients receiving biocomplex compared with the group of animals exposed to radiation alone, although did not reach con�roll values.

To confirm the nature of changes in the morphofunctional state indicate epididymal spermatozoa in different experimental conditions will look at examples of quantitative evaluation of morphofunctional state indicate epididymal sperm using the criterion of IMFS, using the table 1 and the formulas (1-3).

Example 1. Control group

Here as the experimental group (in the numerator) would speak for itself control group, so the coefficients A and b will be equal to one, which corresponds to 100% effectiveness standard.

1) the Percentage of normal sperm cells relative to control:

2) the Percentage of motile sperm relative to control:

3) IMPS=A+b=1+1=2

Conclusion: the reference value IMFS in the absence of experimental effects, when the indices A and b equal to one (reference standards), has a value of 2.

Example 2. The influence of selenium-containing biocomplex

Animals received selexin and ascorbic acid at doses of 1.5 and 500 mg/kg of body weight in accordance with the schema defined for the second experimental group.

1) the Percentage of normal sperm cells relative to control:

2) the Percentage of motile sperm relative to control:

3)IMPS=A+b=1,1+1,1=2,2

Conclusion: the improvement in IMPS compared with the control through the consumption of selenium-containing biocomplex reflects the fact that improving the efficiency of spermatogenesis.

Example 3. The influence of microwave radiation

Animals were exposed to microwave radiation with a frequency of 42 GHz in accordance with the scheme developed for the third experimental group.

1) the Percentage of normal sperm cells relative to control:

2) the Percentage of motile sperm relative to control:

3) IMPS=A+b=0,55+0,74=1,29≈1,3

Conclusion: Microwave radiation with a frequency of 42 GHz in the given exposure caused a decline of IMPS to the value of 1.3, which reflects the negative effects of radiation on the morphology and kinetics indicate epididymal spermatozoa.

Example 4. The influence of microwave radiation on the background of Biocorrector.

The male albino rats was orally given salesin and ascorbic acid at doses of 1.5 and 500 mg/kg body weight in 50 days. 14 days after the start of administration of selenium-containing biocomplex began to influence microwave radiation with a frequency of 42 GHz (λ=7.1 mm) within 30 days of 30 minutes daily. Irradiation was performed in the seed chamber, made of a material not shielding electro�ignitee radiation. After the effects of microwave radiation background and the introduction of selenium-containing biocomplex in animals was evaluated by morphological and kinetic parameters indicate epididymal sperm by the formulas (1-3) on the basis of the indicators shown in table 1.

1) the Percentage of normal sperm cells relative to control:

2) the Percentage of motile sperm relative to control:

3) IMPS=A+B=0,85+0,82=1,67≈1,7.

Conclusion: the obtained value of IMPS exceeds that for the group of animals exposed to only microwave radiation (EMFS=1,3), although somewhat lower than the control values IMPS equal to 2. This circumstance testifies in favor of the efficiency of correction of spermatogenesis by using the proposed selenium-containing biocomplex in terms of exposure to microwave radiation of extremely high frequencies.

The obtained values of IMFS also allow to calculate the average coefficients of sperm quality (CSC) in percent, taking into account the morphology and kinetics indicate epididymal sperm by the formula (4).

Then we get the following average coefficients of sperm quality for the above examples.

1) Control group:

2) the Impact serenaders�embracing biocomplex:

3) the Influence of microwave radiation:

4) the Effect of microwave radiation on the background of Biocorrector:

In the aggregate all of the data for the calculation of IMPS and CSC are shown in table 2.

Thus, in terms of exposure to microwave radiation indicator of IMFS has a value of 1.3, while in the background of consumed animal selenium-containing biocomplex IMFS increases to a value of 1.7. Therefore, the value of IMPS more than 1.3 in terms of the effects of microwave radiation on the background of consumed Biocorrector demonstrates effective correction of spermatogenesis specified biocomplex that is also confirmed by the data of morphological analysis sections of testicular tissue (Fig. 2, 3). The value of IMFS less than or equal to 1.3, speaks of ineffective correction of spermatogenesis in terms of exposure to microwave radiation of extremely high frequencies.

The structure of the convoluted tubules of the testes of animals exposed to microwave radiation, is shown in figure 2 (paint hematoxylineosin; took. 200x). In terms of exposure to microwave radiation was observed vascular congestion of the testes and the total reduction of germ cells. Was observed chaotic s�agenie of spermatogenic epithelium on the background in some cases the detachment of the basal membrane. In some cases, there were multiple breaks in the basement membrane, zapustevanie seminiferous tubules or uneven height of seminiferous epithelium, which generally allows us to speak about the signs of necrosis of seminiferous epithelium (Fig. 2). The structure of the convoluted tubules of the testes of animals exposed to microwave radiation and pre-treated with selenium-containing bio-complex, is shown in figure 3 (paint hematoxylineosin; took. 200x). Choose the seminiferous tubules have the correct rounded shape. Prior administration of a biocomplex contributed to a marked reduction of the destructive effects caused by only one microwave radiation. Seminiferous tubules are filled with different types of spermatogenic cells. Closer to the lumen of seminiferous tubules can be seen sperm. The height of seminiferous epithelium visually significantly higher compared with that in the control group of animals subjected to only microwave radiation.

Thus, the proposed method estimates the correction of spermatogenesis in terms of exposure to microwave radiation allows to make a conclusion about the effectiveness of the applied fluid on the basis of integrated index that takes into account morphological and kinetic characteristics indicate epididymal spermatozoa. The proposed SPO�obom also possible to quantify the efficiency of spermatogenesis in different experimental conditions.

A method of evaluating the efficiency of correction of spermatogenesis in animals in conditions of exposure to microwave radiation, which consists in the quantification of germ cells of the testis, characterized in that the male albino rats orally administered selexin and ascorbic acid, respectively, at doses of 1.5 and 500 mg/kg animal body weight of 1 times a day for 50 days, and after 14 days on the background of the introduction of selenium-containing biocomplex is exposed to microwave radiation with a frequency of 42 GHz (λ=7.1 mm) within 30 days of 30 minutes daily, then at the end of the scheme of the experimental impact estimate corrective properties of biogas plant in relation to the morphofunctional state indicate epididymal sperm by the formula:
IMPS=A+b, where
IMPS - the index of the morphofunctional state,
A - the proportion of normal spermatozoa compared to the control,
- Fraction of motile sperm relative to control,
and when values of IMFS less than or equal to 1.3 judged ineffective correction of spermatogenesis, while IMPS more than 1.3 judged on effective correction of spermatogenesis in terms of exposure to microwave radiation.



 

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EFFECT: high accuracy and simplicity of the method.

FIELD: medicine.

SUBSTANCE: method involves applying integrated diagnostics approach with blood serum examination being applied using infrared spectrometry methods. Sample absorption spectra in the area of 1200-1000 cm-1 are recorded, absorption peak heights having maximum at 1170, 1165, 1140, 1125, 110, 1070, 1025 cm-1 points are measured. Then, mean peak height C and ratio X of peak height at 1125 cm-1 point of maximum to C are calculated. X≥0.7 being the case, light myocardium injury degree is to be diagnosed. 0.7>X≥0.53 being the case, moderate severity degree case is to be diagnosed. X<0.53 indicates grave injury to take place.

EFFECT: high reliability of diagnosis.

FIELD: analytical methods in medicine.

SUBSTANCE: cells of tested organs are subjected to alternating cytological electrophoresis. When cell vibration amplitude rises by at least 20%, drug is regarded to be appropriate for treatment.

EFFECT: increased drug selection efficiency.

2 ex

FIELD: medicine, hepatology.

SUBSTANCE: the present innovation deals with detecting bilirubin content both before and after curative starvation, moreover, curative starvation one should introduce antipyrine at the dosage of 1 mg/kg, in a day it is necessary to detect antipyrine content in saliva and urine and bilirubin content and at bilirubin content being 30-40 mM/l one should starve at daily intake of 400-450 kcal/d for 3 d, then one should again detect bilirubin level and at its increase by 50-100% one should again introduce antipyrine at the dosage of 1 mg/kg and according to its delayed half-life by 20-50% it is possible to diagnose Gilbert's disease.

EFFECT: higher accuracy of diagnostics.

3 ex

FIELD: medicine, otorhinolaryngology.

SUBSTANCE: the present innovation deals with treating diseases of the upper respiratory tract. One should detect disorders of mucociliary transport (MCT) due to measuring the motor parameters of ciliated epithelium cilia, at least, the rate of mucociliary transport that includes material sampling for further testing as ciliary epithelial cells and registration of cilia fluctuations in human respiratory tract due to TV microscopy performed in patient's lifetime. Material for testing should be sampled from mucosal surface of respiratory tract, and therapeutic tactics should be chosen depending upon motor velocity of cilium's tip obtained based upon mathematical modeling followed by calculations by the formula: , where Vc - velocity of cilium's tip [mcm*sec1]; C1, C2, α - calculated as average values being characteristic for every of human body states under investigation: healthy persons, patients with either acute or chronic forms of sinusitis by the developed model of cilia movement; C1 - curvature of cilium's working length, constant being equal to 1/38 mcm-1; C2 - cilium's curvature from its working length up to the tip [mcm-1]; α - cilium's inclination angle against vertical position [degrees]; π = 3.1417 - constant; L, Ts and Te - parameters measured individually in every patient; L - cilium's length [mcm]; Ts - time from the onset of movement till maximal straightening of cilium's tip [sec]; Te - time of efficient impact [sec]. Moreover, the value being Vc = 9.6 - 11.3 mm/min corresponds to normal MCT functioning. For patients with chronic purulent sinusitis beyond exacerbation accompanied with MCT disorder it is necessary to prescribe complex therapy: surgical or conservative therapy with preparations of secretolytic and secretomotory action. For patients with chronic purulent sinusitis beyond exacerbation without any MCT disorder one should prescribe surgical therapy. All patients with acute sinusitis with MCT disorder should be prescribed complex conservative therapy with preparations of secretolytic and secretomotory action. The method enables to specify optimal therapy based upon scientifically proved indications of investigations by taking into account individual peculiarities of every patient.

EFFECT: higher efficiency.

5 dwg, 3 ex, 2 tbl

FIELD: medical engineering.

SUBSTANCE: device has non-absorbing substrate having hydrophilic target region, which is covered with reagent by applying non-impact micro-drop printing method to produce practically uniform reagent layer. The device is in particular usable for measuring blood coagulation time. Preferential invention embodiment involves determining blood coagulation time by carrying out monitoring of light transition through the target region as the blood sample cover interacts with the reagent.

EFFECT: high reliability of analysis results.

20 cl, 9 dwg

FIELD: medicine.

SUBSTANCE: one should register the values of pH and Redox potential (Red) of liquid means in bio-objects simultaneously. It is necessary to plot a graph ▵pH/▵Red according to fluctuations of variation values in measured parameters at their temporal diagram to transfer it into a columnar one where the height of every column is proportional to the area of this graph between neighboring measurements, correspondingly. According to the ratio of area sums of positive columns to that of negative ones during a certain period of time ( from a minute- to a year-long ones) one should conclude upon a state in a bio-object. The value ranged 0.5-2 is considered to be a standard. The method enables to provide efficient control for a two-phase systemic process.

EFFECT: higher efficiency and accuracy of diagnostics.

1 cl, 9 dwg, 5 ex, 1 tbl

FIELD: medicine, clinical neurology, neurosurgery.

SUBSTANCE: one should perform crystallographic study if cerebrospinal fluid and at protein concentration in liquor samples being from 0.50 g/l and higher at predominance of crystals as suppressed dendrites upon a crystallographic picture one should diagnose a malignant cerebral tumor, and at protein concentration being below 0.50 g/l and predominance of crystals as branched dendrites - a benign cerebral tumor. The innovation enables to fulfill not only due diagnostics of cerebral neoplasms, but detect the degree of tumor process malignancy at the early stages of its development.

EFFECT: higher accuracy of differential diagnostics.

3 dwg, 2 ex, 1 tbl

FIELD: biology, medicine, in particular histological investigation of shells with natural surface by using light-optical microscope.

SUBSTANCE: claimed method includes surface relief investigation by using plane field microscope in reflected light at one-side falling shadow-forming lighting. Light-reflecting ability of specimen surface is provided by silver impregnation. Said impregnation is carried out by specimen hold in 10 % silver nitrogen solution for 10 min followed by reducing thereof with 1 % ascorbic acid solution for 1 min.

EFFECT: method for investigation of natural shell surface relief of improved quality due to visual representation of surface three-dimension image on tissue level.

3 cl, 4 dwg

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