Method for preventing and treating acute radiation disease experimentally

FIELD: medicine.

SUBSTANCE: melanin having water-solubility of at least 80% and an paramagnetic centre concentration of at least 8·1017 spin/g is administered orally into the animals having been exposed to the radiation in a dose high enough to cause a spinal radiation injury; melanin is administered after dissolved in distilled water in the effective concentration. Melanin water is used as drinking water for the mice having been exposed to single and fractionated acute radiation, which is able to cause acute radiation disease. Melanin water is taken from the 1st to 30th day following the single radiation, or from the 1st day of the fractionated radiation to the 30th day on completion of the radiation.

EFFECT: higher survival rate, faster recovered haemopoiesis, body weight and orientation and motion activity.

7 tbl, 5 ex

 

The invention relates to medicine and veterinary medicine and can be used for the treatment of acute radiation sickness.

Melanin is a natural pigment, biologically active substances contained in the tissues of microorganisms, plants, animals and humans (impart color to the hair, the eyelashes, the skin), in a number of foods (tea, coffee, mushrooms, buckwheat, etc.). Formed by polymerization of tyrosine, dioksifenilalanina or of catecholamines on the protein matrix. The chemical structure of natural melanin still has not been established due to their extremely complex composite structure.

According to pharmacological studies, water-soluble melanin is not toxic. Once administered orally or intraperitoneally in the dose range of 100-3000 mg/kg did not cause death of mice. When administered for 15 days there were also no fatalities, violations of the General condition, the pathological changes of internal organs revealed no carcinogenic effect [1-4].

Information available in the literature, demonstrate the existence of melanin diverse biological properties that manifest when administered orally or intraperitoneally. In small laboratory animals (mice, rats) it was shown that melanin has anti-stress [1, 5], anticonvulsant [5] and antimutagenic effect [6, 7]. Fitted�but what the melanin increases physical performance [8], enhances the immune system [1, 8, 9], induces the production of cytokines such as IL-6, IL-8, colornegative factor, the growth factor of vascular endothelium [10, 12]. There is information about the successful application of melanin in the complex therapy of oncological diseases of the lung, colon, breast, diabetes [1]. One of the most important properties is pronounced antioxidant activity. Melanin production inhibit lipid peroxidation due to their ability to capture the superoxide anion radicalO2and peroxide radicals of organic compounds [2, 3, 13, 14, etc.].

In the database literature were found separate sources of information that describe the use of melanin as a radioprotector, parenterally administered up to exposure to ionizing radiation, which is practically impossible in case of sudden emergencies. In experimental conditions once or daily for the 4 days of intraperitoneal administration of melanin before irradiation at doses that cause death 40-80% of mice increased the survival rate of 35% and 60%, respectively [15, 16]. It is also shown that the intravenous injection of nonmelanoma when the radioisotope�Apia melanoma in experiments on mice had no protective effect on tumor cells, but it helped reduce the destruction of hematopoiesis [17 - prototype, 18].

In chronic within 20 days of irradiation of pregnant rats at a total dose ~1,25 Gr, accompanied by the development of acute radiation sickness (ARS), daily intragastric administration of a suspension of melanin in starch gel (10 mg/kg) was eliminated in the postnatal period deficit somatic development in rats [8, 9]. There are indications of the ability of melanin to reduce the accumulation of radionuclides in the body [19].

The objective of this work is to study and proof of optimal preventive and curative (introduction after irradiation) the use of water-soluble melanin after exposure to radiation in doses that cause acute radiation syndrome (ARS). The development to improve the survival of irradiated animals, the acceleration of recovery of body weight, locomotor activity and indicators of blood, irradiated.

The technical result is to increase the survival of irradiated animals, the acceleration of recovery of body weight, locomotor activity and indicators of blood, irradiated.

To achieve these objectives we studied the influence of melanin on survival, indicators of blood and spontaneous motor activity of irradiated mice.

In the course� study revealed, that the best effects are achieved when using melanin in dissolved form. Moreover, the optimal is dissolving it in distilled water at the rate of 12.5 mg per 100 ml, with the use of this water in animals as drinking. We have identified that in this form the melanin is able to provide the most adequate radioresistant effect on already subjected to acute exposure of animals.

In the experiments used the melanin produced in accordance with TU 9197-001-6735746-0, with a water-solubility of not less than 80% and the concentration of paramagnetic centers is not less than 8·1017spin/g.

Therapeutic efficacy of melanin, has been studied in 262 outbred mice CD-1, female weighing 22-30 g. In the experiments were selected only clinically healthy individuals. The control and experimental groups were formed by random selection from an equivalent mass of animals.

Test therapeutic efficacy of melanin conducted in total - acute and fractionated radiation exposure. Short irradiation was performed on x-ray biological RUST-M1 (RUB RUST-M1): voltage of 200 kV, a beam current of 2×2.5 mA, aluminum filter 1.5 mm. the dose rate of 0.85 Gy/min ±10%. The mice irradiated with gamma-quanta60Co with a capacity of 1 Gy/min to install ROKUS-M Dose of a single irradiation was 5.0; 6,5; 7,0; 7,5 Gr.

p> Fractionated total irradiation at 0.5 Gy or 1 Gy was performed daily for 5 days, then again after 2-day break.

Melanin is dissolved in distilled water at the rate of 12.5 mg per 100 ml, mice received free access to drinking water instead from the 1st to the 30th day after a single exposure, and during fractionated irradiation with 1 day after irradiation and 30 days after irradiation. In the same period untreated control animals consumed distilled water.

The experiments were carried out in compliance with the "Rules of work with experimental animals, regulated by the Ministry of health of the USSR No. 75 of 12.08.1987 G.".

The influence of melanin on the General condition of the irradiated animals was assessed by their 30-day survival and the dynamics of the mass, which was measured 2 times per week, as well as the level of locomotor activity in "open field" and the state of blood.

Statistical processing of experimental data was performed using standard methods of variation statistics. Calculated simple averages and their standard errors in all terms of the survey. About the statistical significance of differences between compared groups of animals was assessed by t-test, χ2and Wilcoxon-Mann-Whitney test.

Provedennyh studies, the authors found, what the melanin when taken after irradiation has a therapeutic effect, manifested in the increase in radioresistance. The results in summary form and in different series are presented in tables 1 and 2. Irradiation at doses of 6.5; 7.0 and 7.5 G of x-rays and gamma rays caused the development of medullary form ARS, after which the vast majority of mice in the control groups (68 of 70) died. The survival of untreated animals when observed for 30 days after irradiation was 20% (SD80) and 0% (in DM100). therapeutic use of melanin with drinking water helped to increase the survival rate to 40% (in DM80) and 8.3% (in DM100). Combined ratio stood at 16.3%, in control - of 2.9%. The difference between groups was statistically significant (χ2, p<0.01).

Monitoring the clinical condition of the animals have also revealed that taking melanin after exposure contributes to maintaining body weight at a higher level than in controls, and accelerated growth in the recovery period. Differences in 18-21 days are statistically significant (table 3).

In addition to monitoring clinical course ARS in experimental mice was determined the state of blood on the 8th day after irradiation by x-rays in su�lethal dose of 5 Gy. Table 4 shows that the mass of the thymus, the spleen weight and the number of endogenous colony-forming units in the spleen from treated animals is significantly higher than that of the control and irradiated mice. Registered differences in the mass of these bodies are statistically significant (p<0.01).

In mice undergoing ARS, application of melanin has a beneficial effect on the condition of locomotor activity and physiological status. When testing in the "open field" after irradiation at a dose of 6.5 Gy most of the indicators (number of crossed squares, the number of stevani, zaglyadyvanie in mink) treated mice were significantly higher level than in controls. The total respectively 94,6±6,7 and 52.8±19,8 (p<0,05) when the value of the physiological norm for this type of animals in our experiments 125,0±8,3. (Table 5)

Therapeutic effect of melanin when fractionated total effects in mice caused clinical manifestations of the lesion, characteristic of bone marrow form of ARS, is illustrated in table 6.

In the conducted researches it is established that in the control group, the survival rate was 43.7%. therapeutic use of melanin increased the survival rate to 10% (p=0.002). The mass increase after the end of exposure in treated animals observed for 7 days, in the control - on the 11th day.

After 30 days after fractionated exposure to behavioral activity of treated mice in the open field was also higher than that of control: 141,5±7.6 and 87,7±44, respectively.

At a lower dose per fraction 0.5 Gy and similar irradiation plan in the dynamics of the influence of melanin on indicators of blood (table 7).

The data presented shows that in the period of greatest bone marrow suppression treatment melanin mitigated radiation-induced changes, contributing to the conservation at a higher level (on 7th day) the number of platelets in peripheral blood (873±10,6×109/l, in control - 715±46,6×109/l; p<0,02), mielokariocitov in the bone marrow (13,7±2,8×106in the control - 8,1±1,17×106; p<0.1), with accelerated recovery of hematopoiesis at day 14 and 21 days.

The analysis of obtained results allows to draw a conclusion about the presence of melanin of therapeutic effect when administered after radiation exposure. 2 models of bone-marrow form ARS shown that the use of melanin, improves survival, reduces the negative effect of irradiation on the dynamics of the mass, the indicators of blood and physical activity./p>

Thus, the proposed method is as follows.

After irradiation is carried out oral administration to animals of melanin in the liquid form. And use the melanin with the water-solubility of not less than 80% and the concentration of paramagnetic centers is not less than 8·1017spin/g, in solution in distilled water at an effective concentration. Water use with melanin in mice as drinking after single and fractionated acute doses can cause acute radiation sickness. Water with melanin is used from the 1st to the 30th day after a single exposure, and during fractionated irradiation with 1 day after irradiation and 30 days after irradiation.

Example 1. Treatment-and-prophylactic radioprotective efficacy study of melanin in the experience of the 32 outbred mice CD-1, female weighing 28-30 g, under the conditions of vivarium on a normal diet.

As a model of radiation damage using fractionated by 10-fold the total irradiation (1 Gy daily for 5 days, then after 2-day break again in the same mode. Irradiation is performed by gamma-quanta60Co on installing ROKUS-M (medium dose rate of 1 Gy/min). Animals irradiated groups of 10-16 heads in plastic boxes. After the 1st fraction of the animals are divided into d�e equal to the mass of the group, one of them gets to drink for free access melanin is dissolved in distilled water (DW) at the rate of 12.5 mg/100 ml, the other group receives the DV throughout the observation period (up to the 30th day after irradiation).

The development of the ARS accompanied by a decrease of body weight, decreased physical activity, feed refusal, loss of neatness, more pronounced in control animals.

Integral assessment of the influence of melanin on for ARS is the survival rate. To 30-day application of melanin ensured the survival of all animals (16 of 16), whereas in the control group survived 7 out of 16.

Thus, therapeutic effect of melanin is manifested in increasing the survival rate to 100% compared with 43.7% of survivors in the control group. The difference between the indices is statistically significant (p=0.02).

Example 2. Therapeutic efficacy study of melanin in outbred mice CD-1, female weighing 22-30 g in standard conditions of vivarium with free access to water. Mice subjected to single total irradiation at a dose of 6.5 Gy x-rays biological RUST-M1 (RUB RUST-M1): voltage of 200 kV, a beam current of 2×2,5 mA, aluminum filter 1.5 mm. the dose rate of 0.85 Gy/min ±10%.

Animals irradiated groups in special plastic containers divided into individual�social cell.

One group of mice immediately after irradiation and further up to the 30th day instead of tap water gets the melanin, which is dissolved in ET rate of 12.5 mg/100 ml. At the same time the control animals consume DV. The experimental and control groups are formed by random sampling from an equivalent mass of animals. Surveillance is carried out within 30 days, after which register the number of surviving mice in the experiment and the control.

The development of the ARS accompanied by a decrease of body weight, decreased physical activity, feed refusal, loss of neatness, more pronounced in control animals. When taking melanin inside the group of treated mice out of 20 survived 8 in the control group - from 10 - 2.

Thus, therapeutic effect of melanin in acute short-term exposure resulted in an increase in survival of 20%.

Example 3. The influence of melanin on the change in mass of a body is the integral indicator of the General condition of the animals, study on 30 outbred mice CD-1, female with initial mass 22-30 g under the conditions of the vivarium. Distribute the animals by weight into 2 groups.

The irradiation is performed with x-rays at a dose of 6.5 Gy to install RUST-M1 (RUB RUST-M1): voltage of 200 kV, a beam current of 2×2,5 mA, aluminum filter 1.5 mm. the dose rate of 0.85 Gy/min ±10%. Animals irradiated groups of 10-15 goals with�special plastic containers. After irradiation, one group gets to drink a solution of melanin in DV rate of 12.5 mg/100 ml, the other DV during postirradiation period of observation within 30 days.

In dynamics 2 times a week and record the changes of body weight. Melanin helps accelerate the growth of body weight and its return to baseline. In the period 18-21 days in treated mice, the weights were 18.9±1,09 and 20.9±1.2 g in the control to 13.5±1,24 g and 15.0±2.9 g the Difference between the indices is statistically significant (student test, p<0,02-0,05).

Example 4. The influence of melanin on postirradiation recovery of hematopoiesis study on 24 outbred mice female CD-1 with initial mass 22-30 g (8 - irradiated treatment, 8 - irradiated (control) and 8 - non-irradiated, biocontrol) under the vivarium on a normal diet.

For modeling of acute radiation damage using x-ray radiation at a dose of 5 Gy to install RUST-M1 (RUB RUST-M1): voltage of 200 kV, a beam current of 2×2,5 mA, aluminum filter 1.5 mm. the dose rate of 0.85 Gy/min ±10%. Animals irradiated groups 8 heads in plastic containers. After irradiation, forming 3 randomized by weight groups, one of which is ad libitum receives DV with melanin (12.5 mg/100 ml), and the other two (control of irradiation and biocontrol) - DV.

On the 8th day after irradiation, animals were sacrificed put�m cervical dislocation and standard methods determine the mass of the thymus and spleen, count the number of endogenous colonies detected after 2 hours of fixation in the liquid Buena.

The weights of organs from treated mice was: thymus - 32±2,9 mg; spleen - 38,9±5.3 mg; irradiated control, respectively, and 22.1±2.0 mg and of 25.8±1.6 mg; in biocontrol - 82,6±14,4 mg and 120,8±26,0 mg. Differences between characteristics of the irradiated treated and control groups are statistically significant (Mann-Whitney test, p<0.01).

The level of endogenous colonies in the spleen of treated animals was 7.4±2.1 that is significantly higher than in the control irradiated group 4,0±0,8.

Example 5. The influence of melanin on approximately research and General locomotor activity were examined for 40 outbred CD-1 female mice weighing 22-30 g, irradiated at a dose of 6.5 Gy x-rays at the installation RUST-M1 (RUB RUST-M1): voltage of 200 kV, a beam current of 2×2,5 mA, aluminum filter 1.5 mm. the dose rate of 0.85 Gy/min ±10%. Animals are randomized by weight, is distributed into 3 groups, one of which is ad libitum with DV gets from the 1st day of the melanin (12.5 mg/100 ml), the other two (control of irradiation and biocontrol - unirradiated) - DV.

At the end of the recovery period (32-34 days) in mice undergoing ARS, as well as in intact (biocontrol) determine the state of spontaneous motor activity in "open field". The mouse is placed in the center of the arena, the floor of which is split�Yong into sectors. During the 3-min counts the number of racks, crossed squares, visits to the center, zaglyadyvanie into the holes in the floor and the total number of actions.

I treated melanin mice total sum amounted to 94.6±6.7 and was significantly higher than that of the irradiated control animals - 52,8±19,8, a value of the physiological norm (biocontrol) 125±8,3. Differences between irradiated animals treated and control groups are statistically significant (Mann - Whitney test, p<0,05). Statistically significant differences are registered including the number of zaglyadyvanie in "mink" (29,5±1,46 and 16.8±5,3) and the crossed squares (51,8±6,0 and 27.8±12,4).

Thus, the melanin increases the survival of animals after single or fractionated radiation exposure at doses that cause the development of bone marrow forms of ARS. The effect is achieved with a course of therapeutic ingestion of drinking water at the rate of 12.5 mg/100 ml. Therapeutic efficacy is also clinically registered as maintaining at a higher level of body weight, approximately-motor activity and some indicators of hematopoiesis.

Radioprotective properties in almost complete safety, simple and convenient method of application allow the use of melanin for solving problems of radiation medicine, in particular for the treatment of bone�Mozgovoy forms of radiation injuries.

Sources of literature

1. Water-soluble vegetable melanin http//factor.net.ua/melanin/vodorasvorimyj-rastitelnyj-melanin.html. 2011.

2. Ostrovsky M. A., Dontsov A. E. Physiological functions of melanin in the body //human Physiology. 1985. T. 11. No. 4. Pp. 670-678.

3. Zherebin Y. M., Bondarenko N. And., Makan S. Y., etc. Pharmacological properties anomaloninae pigments //Reports of USSR Academy of Sciences. 1984. No. 3. series 5. P. 64-67.

4. Borshchevskaya M. I., Vasiliev S. M. the Development of ideas about the biochemistry and pharmacology of melanin pigments //Issues honey. chemistry. 1999. V. 45. vol.1. S. 13-24.

5. Zherebin Y. M., Sava, V. M., Kolesnik, A. A., A. V. Bogatsky Study of the antioxidant properties of anomalina DOKL. 1982. 262. No. 1. P. 112-115.

6. Alekseeva T. N., Eremenko V. A., Kulikov V. A., etc. the Effect of herbal melanin pigment on the clastogenic effects of chemical mutagens in mice //J. "Experimental and clinical pharmacology". 2001. No. 6. P. 51-61.

7. Mosse I. B. //Radiation and heredity: Genetic aspects of radiation protection. Minsk: University publishing house. 1990. 208.

8. Izmestiev O. S., Dubovik, B. V., lark L. P. Experimental evaluation of the radioprotective effect of melanin on somatic development when irradiated in prenatal period of ontogenesis //Radiation biology. Radioecology. 2007. T. 47. No. 6. Pp. 684-689.

9. Izmestiev O. S., Dubovik, B. V., lark L. P. the Experiment�price evaluation neiroembrioprotektornykh properties when irradiated melanin in the antenatal period of development //Radiation biology. Radioecology. 2007. T. 47. No. 6. Pp. 690-695.

10. El-Obeid, A., Al-Harbi S., AL-Jomah N., A. Hassib Herbal melanin modulates tumor necrosis factor alpha (TNF-alpha), interleukin 6 (IL-6) and vascular endothelial growth factor (VEGF) production //Phytomedicine: international journal of phytotherapy and phytopharmacology. 2006./May; 13 (5). P. 324-333.

11. El-Obeid, A., Hassib A., Ponten F., Westermark B. Effect of herbal melanin on IL-8: a possible role of Toll - like receptor 4 (TLR4) //Biochemical and Biophysical research communications. 2006. Jun. 16; 344 (4); 1200-6.

12. Oberg F., A. Hassib, Ahnfelt M. et. al. Herbal melanin activates TLR 4/NF-kappa In signaling pathway //Phytomedicine: international journal of phytotherapy and phytopharmacology. 2009. May; 16 (5). P. 477-484.

13. Grossi G. F., M. Durante, Gvalanella G. et al. Effects of melanin on high-LET radiation response of human epithelial cells //Radiation and environmental biophysics. 1998. V. 37. P. 63-67.

14. Zhdanova N. N. The protective effect of the pigment. The originality of the chemical structure of melanin //Microbiology. 1970. T. XXXXIX. vol.4. Pp. 431-434.

15. Kunwar A., Adhihary V., Jayakumar S. et al. Melanin, a promising radioprotector: Mechanisms of actions in a mice model //Toxicol. Appl. Pharmacol. 2012. Oct. 15; 264 (2): 202-11.

16. Berdyshev, G. D., On the protective action of melanin during irradiation of mice //Radiobiology. 1964. Vol. 4. vol.4. S. 644-645.

17. Schweitzer A. D., E. Revskaya, P. Chu et al. Melanin-covered nanoparticles for protection of bone marrow during radiation therapy of cancer //Int. J. Radiat. oncol. Biol. phys. 2010. V. 78. No. 5. P. 1494-1502.

18. E. Revskaya, Iongco A. M., Celler, R. S. et al. Radioimmunotherapy of experimental human metastatic melanoma with melanin - binding antibodies and in combination with decarbazine //Clin. Cancer. Res. 2009. V. 15. No. 7. P. 2373-2379.

19. Mosse I. B., Plotnikov, S. I., Lyakh p. P. Radioprotector, etc. that reduce the accumulation of radionuclides in the body. //Application to and.with. 06.12.90. №1888566 (116992).

Method of sieves�tics and treatment of acute radiation sickness in the experiment comprising the oral administration to animals of melanin in the liquid form after irradiation, characterized in that the melanin with the water-solubility of not less than 80% and the concentration of paramagnetic centers is not less than 8·1017spin/g, in solution in distilled water at an effective concentration, and water use with melanin in mice as drinking after single and fractionated acute doses can cause acute radiation sickness, while water with melanin is used from the 1st to the 30th day after a single exposure, and during fractionated irradiation with 1 day after irradiation and 30 days after irradiation.



 

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3 cl, 4 dwg

FIELD: chemistry.

SUBSTANCE: invention provides a composition having antioxidant properties in the form of a tablet, comprising an active agent based on nicotinamide adenine dinucleotide in reduced form (NADH) and inert filling agents, characterised by that the active ingredient is a complex which is a mixture of 10 wt % NADH with 63 wt % vegetable fats, 17 wt % beeswax and 10 wt % chlorophyll, and the inert filling agents are in the form of microcrystalline cellulose, Macrogol 6000, intense sweetener and a food flavourant.

EFFECT: invention provides a new tablet form of NADH.

1 ex

FIELD: medicine.

SUBSTANCE: invention refers to a pharmaceutical composition with anti-ischemic and antioxidant activity in the form of tablets or capsules, and a method for preparing it. The composition contains 4-((3-oxo-3-ethoxypropanoyl)amino)benzoic acid in an amount of 40 to 80 wt %, an amino-containing compound and pharmaceutically acceptable excipients. The amino-containing compound is specified in a group of trometamol, methyl glucamine and L-lysine; 1 mole of 4-((3-oxo-3-ethoxypropanoyl)amino)benzoic acid is accounted for 0.05 to 0.25 mole of the above amino-containing compound. The composition also contains lactose, microcrystalline cellulose, calcium stearate and other pharmaceutically acceptable excipients. According to the method for preparing the composition, taking 4-((3-oxo-3-ethoxypropanoyl)amino)benzoic acid and amino-containing compound in molar ratio 1:0.05 to 1:0.25, pre-mixing, moisturising with a aqueous or alcohol solution of a binding agent, adding pharmaceutically acceptable excipients in such an amount to provide the content of 4-((3-oxo-3-ethoxypropanoyl)amino)benzoic acid from 40 to 80 wt %, granulating the mixture, drying and producing tablets or capsules according to the known technique.

EFFECT: implementing the above application.

6 cl, 7 tbl, 4 ex

FIELD: chemistry.

SUBSTANCE: claimed is the application of 5(6)-nitro-1-(1,1-dioxothietanyl-3)-2-chlorobenzimidazole of formula (I) , earlier known as the means with broncholytic and spasmolytic activity, as the means, inhibiting peroxide oxidation of lipids.

EFFECT: realisation of the claimed purpose.

1 dwg

FIELD: chemistry.

SUBSTANCE: claimed is the application of 5(6)-nitro-2-chloro-benzimidazole of formula (I) as the preparation, inhibiting peroxide oxidation of lipids.

EFFECT: achievement of the claimed purpose, activity of the said formula compound is higher than of the comparison medication dibazole.

1 dwg

FIELD: medicine.

SUBSTANCE: method for preparing an agent possessing anti-inflammatory, diuretic and antioxidant activity, involving milling Spiraea salicifolia shoots representing a mixture of leaves, blossom and shoots, extracting them three times by gradual maceration, mixing in infusing, filtering, condensing, separating, drying in the certain environment.

EFFECT: agent shoes the pronounced anti-inflammatory, diuretic and antioxidant activity.

2 dwg, 12 tbl

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to medicine and can be used as a hepatotrophic, lipotropic agent in gastroenterology, as well as for treating cardiac and cerebral atherosclerosis, in neurology in the lower extremity artery diseases (endarteritis). The agent contains tabletted powders of diisopropylammonium dichloroacetate, microcrystalline cellulose, lactose and excipients presented by Aerosil, Primogel and calcium stearyl fumarate.

EFFECT: agent possesses no toxicity, has the high adsorption properties, the apparent anti-inflammatory action, and provides metabolism and the liver regeneration.

3 tbl, 2 ex

FIELD: chemistry.

SUBSTANCE: invention relates to heterocyclic compound - 6-methyl-5-morpholynomethyl-1-(thiethan-3-yl)pyrimidine-2,4(1H,3H)-dione of formula 6-methyl-5-morpholynomethyl-1-(thiethan-3-yl)pyrimidine-2,4(1H,3H)-dione of formula: .

EFFECT: novel compound, possessing antioxidant activity, is obtained.

2 cl, 6 tbl, 7 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to medicine, and concerns a pharmaceutical composition for treating gastroduodenal ulcer in the form of tablets, capsules or gel, containing therapeutic agents and a consistency base applicable for each dosage, wherein the therapeutic agents are as follows: recombinant interferon specified in a group: recombinant interferon alpha, recombinant interferon beta, recombinant interferon gamma; antiseptics; amino acids specified in a group: arginine, histidine, lysine, cysteine, methionine, glutamic acid; and antioxidants specified in a group: beta-carotene, vitamin C, vitamin E.

EFFECT: invention has the integrated body effect promoting faster healing of the mucosal defect accompanying the aggravated gastroduodenal ulcer and preventing recurrences, including by the eradication effect.

6 cl, 5 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to medicine, and concerns a pharmaceutical composition in the form of tablets for oromucosal administration in treating infectious-inflammatory oropharyngeal diseases. The composition contains recombinant interferon specified in a group: recombinant interferon alpha, recombinant interferon beta, recombinant interferon gamma; amino acid specified in a group: arginine, histidine, glycine, glutathione, lysine, methionine, cysteine; an antiseptic, a preserving agent, flavours specified in a group: tea tree oil, eucalyptus leaf oil, mint leaf oil and a consistence base.

EFFECT: invention makes the composition applicable in fragile and frequently ill patients for reducing a disease recurrence.

7 cl, 1 ex

FIELD: food industry.

SUBSTANCE: biologically active food additive strengthening the organism adaptive power and body defences and having anti-inflammatory and antioxidant activity contains vegetal origin components represented by a complex extract of devil's-club root, Rhaponticum carthamoides root, Hedysarum neglectum Ledeb root, celery roots and leaves, rhodiola rosea root, Japanese angelica tree roots, boschniakia rossica roots, Hungarian sainfoin herb, magnolia-vine fruits; additionally the additive contains chitosan, trepang fermentative hydrolysate, ascorbic acid, taurine, glutathione, nicotinamide, vitamin B1, vitamin B2, vitamin B6, vitamin B12, folic acid, anhydrous calcium chloride, magnesium chloride, zinc chloride, bee honey at preset ingredients ratio.

EFFECT: biologically active food additive promotes effective strengthening of the organism adaptive power and body defences and human aging retardation.

4 tbl, 6 ex

FIELD: medicine.

SUBSTANCE: invention refers to medicine, more specifically to clinical dentistry. The invention represents a composition for treating erosive ulcerative and exudative hyperaemic forms of oral lichen planus.

EFFECT: implementing the invention provides high oral adhesion of the preparation, increases the therapeutic concentration of the preparation in the area of involvement (inflammation), accelerates the length of treatment (reduces the size of erosion, the length of epithelisation of erosions).

2 dwg

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