Solution for fixing biological cells
SUBSTANCE: invention relates to a solution for fixing biological cells. The fixing solution is intended for in vitro preservation of a cytological sample containing nucleated cells and erythrocytes. The solution contains 80-95 vol. % of a mixture of 590 ml normal saline, 10 ml polyethylene glycol (Carbowax®), 203 ml isopropyl alcohol, 193 ml pure ethanol, 0.01 vol. % sodium azide, and 5-20 vol. % buffered 4% formalin; pH of the fixing solution is in the range of 6.4 to 7.4.
EFFECT: preserving the integrity of nucleated cells.
2 cl, 2 dwg, 1 ex
The present invention relates to a solution for fixing biological cells (fixative solution) intended for in vitro conservation of the cytological specimen, the type containing alcohol solution for fixing cells. More specifically, the invention relates to the field of Cytology.
The fixing solutions or the latches provided for the preservation and conservation of cytological samples, including cells, for subsequent analysis by a pathologist. Cell samples can be, for example, obtained by the puncture needle by washing, scraping or collecting urine from the patient. The cells can be cells of any organ, such as the uterus, liver, stomach, mammary gland, and the like. The sample of cells may be, for example, a sample of fluid, more specifically, urine, ascites, pleural or pericardial fluid; smear, more specifically, cervical smear, smear from the vagina; the puncture body, more specifically the outer body, such as mammary gland, thyroid gland, or an internal organ, such as pancreas or liver and the like.
"Cytological fixative or cytological fixing solution" means a solution commonly used cytological assays for cell fixation.
The commit operation is designed for maximum feasible�th preservation of the morphology of cells in the state in which they were prior to their selection. The best clips are the ones that, providing a fast action, cause the minimum possible number of secondary or artificial changes which can interfere with the analysis of the internal morphology of the cells.
Known and has long been used, particularly in the agricultural and veterinary field, formaldehyde acetic alcohol or AFA. AFA contains methyl or ethyl alcohol, formalin and glacial acetic acid. Formalin is a mixture consisting of formic acid, formaldehyde, paraformaldehyde and methanol. An example is the AFA company Locquin, having the following composition:
|Ethyl alcohol strength 80°||100 cm3|
|Laboratory formalin concentration of 38%||10 cm3|
|Glacial acetic acid||5 cm3|
|Distilled water||20 cm3|
Thus, glacial acetic acid destroys red blood cells and dissolves their contents, resulting in formation of deposits of hemoglobin, very disturbing �ri analysis after standard staining, more specifically on the smear, or staining by May-Grunwald-Giemsa (MGG), or very disturbing when immunocytochemically or other studies. Moreover, from the perspective of normative acts with this concentration, ethanol is considered to be a flammable substance, and the formalin is toxic and carcinogenic substance.
The purpose of the invention is to provide a solution for fixing biological cells, to ensure good preservation of the integrity of nucleated cells and red blood cells for analysis, but less dangerous for the user.
In this regard, an object of the invention is the solution for fixing biological cells of the above type containing the saline solution to prevent osmotic shock on the level of the cell wall, formaldehyde and polyethylene glycol to preserve the size and integrity of nucleated cells and erythrocytes, fixed mentioned solution.
According to other aspects of the invention the solution for fixing biological cells possess one or more of the following properties:
the fixing solution contains no acetone or compounds of the class of ketones or acetic acid to maintain the integrity of red blood cells.
- alcohol is ethanol or isopropanol;
- alcohol is a mixture of ethanol and isopropanol;
- the number of alcohol 45% by about�jemu fixative;
the amount of formaldehyde in substance is from about 0.2 to 1% by volume of fixative.
the fixing solution contains a buffer, ensuring that the pH of the fixing solution is essentially from 6.4 to 7.4; and
the fixing solution contains from 80% to 95% by volume:
- 590 ml of saline,
To 10 ml of polyethylene glycol (Carbowax®),
- 203 ml of isopropyl alcohol,
- 193 ml of pure ethanol,
- 0,01% sodium azide,
and from 20% to 5% by volume of formalin, buffered at the rate of 4%.
So, this solution provides better preservation of cell ratios, more specifically the size of the nucleus relative to the size of the cell, and more specifically the excellent preservation of erythrocytes.
In addition, this fixing solution slavophones, non-toxic and not carcinogenic.
The invention will be better understood when reading the description below is only an example and made with reference to the drawings, in which:
- figure 1 is a photograph of a sample of cells obtained from breast cancer, which was retained in the fixing solution according to the invention,
- figure 2 is a photograph of a sample of cells selected from the thyroid gland, which has been stored in fixative solution according to the invention.
The present invention relates to a fixing solution, PR�naznachennomu for in vitro conservation of the cytological specimen, containing biological cells intended for analysis by a pathologist.
The fixing solution contains an isotonic solution of sodium chloride or saline instead of distilled or deionized water is normally used as the basis of traditionally used fixing solution.
Saline has long been known, it contains sodium chloride dissolved in distilled water at the rate of 9 grams of sodium chloride in 1 liter of water. Saline is ISO-osmolar that supports cells able to correct osmolarity, prevent any osmotic shock on the level of the cell wall and, consequently, the destruction of cells by rupture of the cytoplasmic and nuclear membranes. The osmolarity of normal saline is 308 mosmol ' per liter.
Osmolarity is a concentration of the medium. This brings us to the concept of osmosis which is the diffusion of solvent through a semipermeable membrane separating two solutions with different concentrations, for example, the fixing solution and the cytoplasmic fluid of the cell.
In addition, the fixing solution contains alcohol for maintaining the cells in their condition, locking them in alcohol medium.
Preferably, the amount of alcohol 45% by volume of fixative so that it was slavophones. Thus�Ohm, it is a small amount of alcohol makes it easier to transport and store the fixative solution, because it is less dangerous. More specifically, under the weak Flammability imply that the solution according to the invention does not require application to the packaging solution for a security icon, warning about the Flammability of the product.
More specifically, the alcohol is ethanol or isopropanol.
According to one of the embodiments of the invention, the alcohol is a mixture of ethanol and isopropanol.
However, alcohol is dehydrating, reducing substance, thus changing the size of cells due to reduction of nucleus and cytoplasm.
To compensate this disadvantage of fixing solution also contains formalin to preserve the size of the cells. The fact that the fixing solution may also contain Carbowax® or other polyethylene glycol, known and published Saccomanno (Saccomanno) and colleagues. Known and publicized the fact that formalin can be combined with substances that remove calcium salts or antiplatelet agents such as ethylenediaminetetraacetic acid (EDTA) and its salts. Also known and publicized the fact that the samples containing mucus, you can add a mucolytic substance, in particular dithiotreitol (DTT) or acetylcysteine.
Formalin allows you to maintain the red blood cells and nuclear cells, not on them and ensuring that the ratio in size that allows the cytologist to have more accurate diagnosis.
More specifically, as can be seen in figures 1 and 2, samples of cells that were stored in the fixative solution according to the invention, the red blood cells, marked by the numeral 1 in the figures, perfectly preserved and not compromised, which allows a precise analysis of these samples.
Preferably, the amount of formaldehyde in substance is from about 0.2 to 1% by volume of fixative. With this concentration, formalin or formaldehyde is considered as a simple irritant tool on Toxicological registry issued by the National Institute of research and safety (INRS), in contrast to the commonly used concentrations from 1 to 25%, in which the formaldehyde is corrosive and carcinogenic. Thus, the packaging solution of the invention also does not require the application of security badge, alerting you that the product is corrosive.
Therefore, when the concentration is below 1% with a solution you can safely make the manipulation, as the level of precautions for performing manipulation with it is below.
It is known that the fixing solution may contain an antimicrobial additive.
The fixing solution contains a buffer, ensuring the pH of the fixing solution essentially from 6.4 to 7.4. This pH range corresponds to about�optimal conditions of storage cells and blood of the human body.
The following examples illustrate the invention without limiting its scope.
A solution formed of 80% by volume of:
- 590 ml of saline,
To 10 ml of polyethylene glycol (Carbowax®),
- 203 ml of isopropyl alcohol,
- 193 ml of pure ethanol,
- 0,01% sodium azide,
and 20% by volume buffered 4% formalin.
In one embodiment, the solution can be formed from 90% by volume of the above mixture and from 10% by volume buffered 4% formalin or 95% by volume of the above mixture and of 5% by volume buffered 4% formalin.
It should also be noted that the fixing solution according to the invention contains no acetone or compounds of the class of ketones or acetic acid. These products lisarow red blood cells, with destruction which releases hemoglobin, which is deposited on the cells. Some types of staining, such as staining of PAP smears, given the complexity of dyes that combine multiple nuclear dyes and hemoglobin do in this case, cytological analysis, and more specifically the study of the nucleus is very difficult, if not impossible. Immunocytochemical studies also often obstructed by deposits of hemoglobin.
The use of a fixative solution according to the invention improves the subsequent analyses of the sample with staining on Papanek�Lau or Immunocytochemical examination fixed in fixation solution described in this document and does not contain any acetone or compounds of the class of ketones or acetic acid.
Thus, as described above, the fixing solution provides excellent preservation of the integrity of nucleated cells and red blood cells for analysis.
1. The fixing solution used for in vitro conservation of cytological sample containing nuclear cells and red blood cells, characterized by the fact that it contains from 80% to 95% by volume:
- 590 ml of saline,
To 10 ml of polyethylene glycol (Carbowax®),
- 203 ml of isopropyl alcohol,
- 193 ml of pure ethanol,
- 0,01% sodium azide,
and from 20% to 5% by volume buffered 4% formalin.
2. The fixing solution according to claim 1, characterized in that it comprises a buffer providing a pH of the fixing solution from 6.4 to 7.4.
SUBSTANCE: static, dynamic or vibration sensing is carried out preliminary at the selected points to the depth from 1 m with respect to the top of the earth fill. At the same time the samples of compacted soil of undisturbed structure are selected in order to determine the moisture and density of skeleton of the specified soil from several drilled wells at points at a distance of not more than 1 metre in plan from sensing points. Laboratory researches of standard compaction with definition of compacting factor depending on the density of soil skeleton, are carried out on the selected samples of soils from the body of compacted fill. Construction of correlation dependence is performed between the specified values of compaction factor and values of the resistance to penetration of standard cone into the soil during sensing, taking into account determinations previously performed in the laboratory followed by evaluation of compaction quality of the earth fill.
EFFECT: improving the accuracy of definition and identifying the areas of non-compacted soil for its subsequent local postcompaction.
SUBSTANCE: method includes the preparation of smear from peripheral blood with preliminary fixation with methyl alcohol, drying, washing with distilled water. After that, the smears are placed in a potassium chloride solution in a ratio of 0.57 g of potassium chloride per 100 ml of distilled water for 20 min and washed with distilled water. Additionally prepared is a mixture of solutions, prepared ex tempore, containing a solution "A" and "B". The solution "A" includes a 50% silver nitrate solution in an amount of 5 g of silver nitrate + 5 ml of distilled water. The solution "B" includes a 2% solution of gelatin on a 1% formic acid solution in an amount of 15.8 ml of distilled water + 0.2 ml of 100% formic acid + 4.0 ml of 10% gelatin. The solutions "A" and "B" are mixed in an amount of 5 ml of each, in darkness, with further submergence of the blood smears for 20 min in darkness in the thermostat at a temperature of 37°C with the further submergence of the smears into distilled water for 2-3 seconds. After that, they are twice subjected to a 8 min exposure in a 5% sodium thiosulphate solution in darkness in the thermostat at a temperature of 37°C. After that, they are washed successively with tap water and distilled water, after-staining is performed in the Romanovskiy dye for 30 min. After that, the smears are washed again with tap water, air-dried, placed in the Canadian balm and covered with a coverslip.
EFFECT: increased quality of smear staining and provision of a possibility to identify and further evaluate parameters of nucleolus organiser regions.
4 tbl, 6 ex
SUBSTANCE: method of selection of horizontal soil monolith comprises embedding along the genetic horizons of nth thin-walled metal cylinder-monolith-selector of the ith diameter with a pointed lower end of a triangular shape. The selection of the horizontal soil monolith from the pit is carried out with the number of cylinders-monolith-selectors k, equal to where i - number of the cylinder diameter (n > i > 1), n - number of cylinders of different diameters, ki - the number of repetitions of the cylinder of ith diameter (ki > 3). And each time, prior to selection of the horizontal soil monolith the inner surface of each used cylinder-monolith-selector is greased with petroleum jelly, and the load on the cylinder-monolith-selector is performed in a direction perpendicular to the surface of the pit stepwise with fixing the load of each step. The set of devices for selection of the horizontal soil monolith comprises the said k-th number of thin-walled metal cylinder-monolith-selectors and a metal cylindrical nozzle on the cylinder-monolith-selector. The metal nozzle is provided with a cylindrical recess in one of its ends, which diameter is equal to the outer diameter of the cylinder-monolith-selector having a maximum diameter of n cylinder-monolith-selectors, and the axis of symmetry coincident with the axis of symmetry of the metal cylindrical nozzle. The set also comprises (n-1) washer with an outer diameter equal to the diameter of the recess and the thickness equal to the height of the recess in the end of the metal cylindrical nozzle with the ability of mounting of each of them to the recess, followed by fixing in it. The inner washer diameters are different and equal to the outer diameter of each of the (n-1) cylinder-monolith-selector, constituting a pair: washer-cylinder-monolith-selector. Set is provided with a screw press with a head and a heel of cylindrical shape and a shield with a recess on its axis of symmetry with the ability of mounting in it through the heel of the screw press. And in the other end of the metal cylindrical nozzle on the cylinder-monolith-selector on its axis of symmetry a recess of cylindrical shape is made, the diameter and depth of which correspond to the diameter and thickness of the head of the screw press.
EFFECT: improvement of quality of sampling soil of undisturbed placement and increase in the accuracy of determining the water-physical and filtration properties of soil on genetic horizons of the soil profile, reduction of time for selection of the monolith and the complexity of operations in selection of quality horizontal soil sample.
3 cl, 1 dwg, 1 tbl
FIELD: engines and pumps.
SUBSTANCE: device comprises a floating element 10, which is placed onto the sea surface and connected to a pump, rigidly fixed to the sea bottom or to massive floatage 8. The pump is made in the form of a cylindrical pipe-shaped vertically arranged chamber 1 semi-submerged into the sea, which in its upper and lower parts is equipped accordingly with lower 3 and upper 6 nozzles. At the lower nozzle 3 there is a hose 4 with certain length arranged in water depth. In the chamber there is a piston in the form of an inlet check valve placed on the stem 9, which is made as capable of passing water in the chamber only in direction from the lower nozzle to the upper one and is connected by means of the stem 9 with a floating element 10. The piston may be made within a membrane 12 adjacent to the plane of a disc 11 made with through holes, axes of which are parallel to the axis of the disc.
EFFECT: simplified design, expanded area of application of a device for water lifting.
2 cl, 1 dwg
FIELD: measurement equipment.
SUBSTANCE: invention relates to equipment for determining consumptions and periodic water sampling from different horizons of a peat deposit, which are fixed as to depth. The complex includes a well casing pipe with a cone tip and a water intake structure. Besides, a sampling unit includes a cylindrical housing, on which there located are two elastic rubber cuffs with diameter equal to well diameter; in the wall of the cylindrical housing there are side holes - a middle one - for water receiving from a working horizon and is located between two cuffs; an upper one is located above the upper cuff; the lower one is located under the lower cuff; upper and lower holes are of a transit type and connected to each other with a tube passing inside the cylindrical housing of the sampling unit; the lower part of the cylindrical housing is connected to the water intake structure through a flange attached to the cylindrical housing; the upper part of the cylindrical housing is connected to a bracket for lifting the sampling unit and the water intake structure connected to it, the diameter of which is lower than inner diameter of the well casing pipe; the well casing pipe is pipes from one to N, which are connected to each other with external threaded couplings and side holes made throughout length of the pipes.
EFFECT: simpler design.
2 cl, 2 dwg
FIELD: measurement equipment.
SUBSTANCE: invention relates to a measuring probe for measurement and taking samples in molten metal. The probe is provided with a measurement head located on a rod, which includes at least a temperature sensor and a sampling chamber. The latter is at least partially enveloped with the measurement head and includes an input channel passing through the measurement head. The input channel has an internal section with length L, which is located in the measurement head, and has minimum diameter D at least at one point in this internal section; with that, L/D2 ratio is less than 0.6 mm-1. Besides, the measurement head has counter pressure Pg of lower than 20 mbar, which is determined so that first a reference gas flow is passed via a pipe with two open ends, and pressure P1 is measured in the pipe. Then, the pipe is inserted with one end into the inlet channel of the measurement head; the same reference gas flow is passed via the pipe and pressure P2 is measured in the pipe, and counter pressure Pg of the measurement head is determined based on difference P2-P1.
EFFECT: improvement of quality of the obtained samples.
23 cl, 5 dwg
SUBSTANCE: apparatus includes a receptacle in the form of a sealed container whose lower part is fitted with a controlled two-way diaphragm valve, and a nozzle for feeding water used to wash the sample delivery line. The apparatus also includes a filter element, which is hermetically connected to a filtrate storage container, and an information control, display and transmission unit. The filter element is located in the working medium and is mounted on the sample removal line. To regenerate the filter surface, the apparatus includes a hydraulic pressure pulsator, which is installed on the sample removal line between a ball valve and a resistance disc, through which the sample is fed into the sample receptacle in the form of a sealed container, having a water-cooled jacket located at the end of the filtrate removal line.
EFFECT: invention improves the accuracy of monitoring process parameters, provides timely detection and rectification of process disorders, which enable to obtain more reliable data on the chemical composition of a solution.
SUBSTANCE: blood is sampled, acidified to pH 2-3 with aqueous oxalic acid, extracted in toluene for 5 min; the prepared extract is centrifuged for 60 min at 7,000 rpm, added with sodium sulphate to dewater and acetylated for 3 hours by introducing trifluoroacetic anhydride while stirring continuously in the pyridine medium. The blood sample, toluene, trifluoroacetic anhydride and pyridine are taken in volume ratio 5:2.5:0.2:0.1 respectively.
EFFECT: simplifying the stage of sample preparation and increasing the sensitivity of pentachlorophenol test.
3 cl, 4 tbl, 1 ex
SUBSTANCE: described are: solution for preliminary processing for immunohistochemical staining, which elutes paraffin-containing mounting medium from microscope slide with tissue sample, embedded into medium, and extracts antigenicity of tissue sample, and which can be used three or more times, and solution concentrate for preliminary processing for immunohistochemical staining, which provides possibility of easy obtaining of solution for preliminary processing. Solution for preliminary processing for immunohistochemical staining contains antigen-extracting agent, certain non-ionic surface-active substances in specified range and cyclodextrin or its derivative in certain amount, with not less than 80% of water constituting the remaining part. Composition of antigen-extracting agent is such that pH of solution for preliminary processing is in specified range, and content of cyclodextrin or its derivative represents specified amount.
EFFECT: obtaining solutions for preliminary processing for immunohistochemical staining.
21 cl, 5 tbl, 11 ex
FIELD: test equipment.
SUBSTANCE: invention relates to laboratory test equipment, namely to the device for forming and testing of samples of thin coatings in loading devices, for example, for testing of thin ceramic heat-shielding coatings for tensile strength. The device is a two-piece unit intended for placement in the load device comprising two cylindrical and circular details the external surface of which is intended for application, at least, of one layer of thin coating and forming of a sample. One of cylindrical details has on the axis a cylindrical cavity, and another one a companion cylindrical ledge placed through a ring hole in a cavity and connecting the details. The external surface of cylindrical details has adhesion, and a ring surface has applied coatings without adhesion, and serve, respectively, for forming of a sample as a connecting layer and/or non- adhesion thin coating.
EFFECT: improvement of reliability of study of strength properties of thin coatings by forming of non-adhesion longitudinal superficial sample on the two-piece unit suitable for loading by longitudinal and temperature loads.
5 cl, 3 dwg
SUBSTANCE: coloured X-ray contrast mass consists of barium sulphate, glycerine, acrylic dye and aqueous gelatine solution. The invention relates to methods of producing a setting coloured X-ray contrast mass. The disclosed mass can be used for macro- and micro-preparation of vessels and X-ray imaging thereof. The preparation method includes thoroughly mixing barium sulphate, glycerine, acrylic dye and aqueous gelatine solution, wherein barium sulphate, glycerine, acrylic dye and aqueous gelatine solution are taken in amount of 1.5 parts, 1 part, 0.1 parts and 7.5 parts, respectively, stirring and heating the mixture to 60-80°C before application. The prepared mass has high permeability in thin vessels, brightness (clarity), fast setting and elasticity. The mass is prepared from readily available and cheap materials. The remaining or unwanted mass can be stored for a long period of time.
EFFECT: disclosed mass can be heated repeatedly and is ready for use.
SUBSTANCE: cryoprotector is opened in a laminar flow unit; a special syringe of asyringe pump is filled with a cryoprotector; that is followed by introducing a filter solution of the cryoprotector - 55% dimethylsulphoxide with 5% dextran 40 at temperature +4°C in a nucleated cell suspension with haemopoietic stem cells in a cryopackage with the concentrate and mixing mechanically in a mixing apparatus, transferring the system together with the cryoprotector flask into the laminar flow unit; the air is released from the cryopackage and portion of the suspension; the package is sealed and placed into a shrink bag; that is followed by programmed multi-stage freezing, the first stage of which keeping the mixture of the suspension with the stem cells and cryoprotector - a freezing sample - for 10 min at temperature +4°C; the second stage is cooled at a rate of 1°C/min to temperature -12°C; the thirst stage provides cooling at a rate of 20°C/min to temperature -60°C; at the fourth stage, the sample is heated at a rate of 10°C/min to temperature -18°C; at the fifth stage, the sample is cooled at a rate of 1°C/min to -60°C; at the end of the freezing program, the sample is cooled at a rate of 3°C/min to temperature -100°C; after freezing, the sample is placed into a quarantine dewar with liquid nitrogen until infection and bacteriological fungal contamination test results are obtained. After termination of the quarantine shelf life, the sample with haemopoietic stem cells are placed for long-term storage at temperature not exceeding -150°C, into the dewar with liquid nitrogen if observing negative test results. If the infection and bacterial and/or fungal contamination test results are positive, the sample with haemopoietic stem cells are transferred into the dewar with liquid nitrogen for infectious material for long-term storage.
EFFECT: invention enables increasing cell viability in the sample.
3 cl, 4 dwg
SUBSTANCE: invention relates to method of obtaining 1,6-bis-(1,5,3-dithiazepan-3-yl)-2,5-disulphanylhexane of formula (1), possessing fungicidal activity against Botrytis cinerea and Rhizoctonia solani. Essence of method lies in interaction of mixture of 1,2-ethanedithiol and formaldehyde with water solution of ammonium salts NH4X (X=F, Br, OAc, NO3, 1/2SO4) with molar ratio HS(CH2)2SH:CH2O:NH4X=3:6:2 at room temperature (~20°C) and atmospheric pressure for 5-7 h.
EFFECT: output of 1,6-bis-(1,5,3-dithiazepan-3-yl)-2,5-disulphanylhexane of general formula (1) depending on applied ammonium salts (NH4X) constitutes 31-67%.
2 cl, 2 tbl, 1 ex
SUBSTANCE: paleontological objects with soft tissues are treated for 1-4 months with an embalming solution containing 40% formalin - 0.516%, crystalline phenol - 0.262%, glycerine - 79.077%, 96% alcohol - 18.576%, sodium chloride - 1.569%. The ratio of the embalming solution to the mass of the paleontological object must not be less than 3:1. Embalming is carried out at room temperature.
EFFECT: method of embalming paleontological objects with soft tissues provides prolonged preservation of soft tissues, minimises loss of soft tissues having different rotting stages, stops rotting processes, skin straightening, preserves natural colour thereof and natural morphometric parameters, which enables further use of the paleontological object for scientific purposes and for exhibition in dry form.
SUBSTANCE: invention refers to physiology, cryobiology and medicine, namely to human blood nuclear cell preservation technique with the use of inert gas. The method involves cell saturation in Compoplast 300 plasticised container placed in a metal cryobarocontainer with inert gas, xenon at pressure 0.6 atm for 20min in the room temperature environment of 21±2°C; excessive gas is eliminated, and the biological object is removed from the metal container and frozen to -28°C in ethanol, placed into an electric freezer at -80°C. The bioobject is unfrozen in a water bath at 39±1.5°C for 1.5 min.
EFFECT: implementing the invention provides the reliable cryonic preservation of leukocytes in the inert gas medium and the high level of qualitative and morphological safety of leukocyte concentrate of human blood.
1 tbl, 3 ex
SUBSTANCE: method of reducing impact of low-temperature jump on cryoprotectant solution is provided at the expense of remote processing of cryoprotectant solution with cells of living organisms by ultrasonic radiation with frequency 0.50-10 before its freezing.
EFFECT: reduction of low-temperature jump of cryoprotectant solutions, which makes it possible to eliminate overcooling effect, provide continuous and smooth character of freezing and increase integrity of defrosted cells after cryoconservation.
SUBSTANCE: what is presented for embalming of dead bodies for the purpose of organ harvesting and transplantation drills is a method, wherein tissues and organs are saturated with embalming solutions; the dead body is immersed into water at t 40°C for 120-160 min; cannulas are inserted into a femoral artery, and a blood flow is washed with warm normal saline with 1-2% sodium citrate added; the femoral artery is sealed, and a solution containing formalin and glycerol is inserted into the artery.
EFFECT: keeping the internal organ structure by increasing the preserving properties of the solution and providing the small vessel filling.
SUBSTANCE: invention relates to a method of lyophilisation of a composition, which contains purified antithrombin III (AT III) and a crystallised substance, selected from alanine, mannitol, glycine or NaCl. The claimed method includes freezing the composition at a temperature from -52°C to -60°C for 6-15 hours, annealing the composition at -30°C for 1 hour, re-freezing the composition at a temperature from -52°C to -60°C for 2-15 hours at keeping the product temperature between -48°C and -52.7°C for 4-10 before lyophilisation and drying the composition with obtaining a lyophilised cake. The invention also relates to a pharmaceutical set, which contains the said lyophilised cake and a liquid reagent.
EFFECT: invention provides obtaining the lyophilised composition, containing AT III, which preserves its activity and stability.
14 cl, 24 dwg, 5 tbl, 2 ex
SUBSTANCE: invention relates to novel compounds of formula
where X=S, SO or SO2, and one of radicals R1 and R2 is a hydrogen atom and the other has a value given in the claim, which are used for depigmentation of the skin and/or hair and/or body hair and for skin disinfection, to cosmetic application of the disclosed compounds, and to compounds of formula (I), wherein R1 and R2 can also be a hydrogen atom at the same time. The invention also relates to a cosmetic composition and a pharmaceutical composition based on the disclosed compounds and to methods of producing said compounds.
EFFECT: improved properties.
19 cl, 2 tbl, 30 ex
SUBSTANCE: preserving the myocardium in transplantation involves perfusion and storage of the isolated heart with using a Custodiol solution at a temperature of 2-4°C. The heart tissue culture into the donor precedes a 10-12-hour infusion of Levosimendan 10 mcg/kg of body weight, while the perfusion and storage of the isolated organ is ensured by adding Levosimendan into the Custodiol solution at 10 mcg of the preparation per 1 litre of the solution. The surgical stage of the heart transplantation after the recipient's aorta de-clamping and the blood flow recovery is followed by introducing Levosimendan 12.5 mg into the continuing bypass circuit and infusing it for 1 hour.
EFFECT: invention aims at preventing the ischemic and re-perfusion myocardial injuries, providing the adequate function of the donor's heart in the recipient's body.
SUBSTANCE: method involves using product containing spermatozoa treated by drying with freezing to humidity level of 1% and having injured membrane or spermatozoon head so that spermatozoon nucleus retains its genetic validity enough for fertilization. The spermatozoon heads fertilize an isolated oocyte after rehydration and microinjection being done. The retained genetic integrity is enough for fertilizing an oocyte and producing living descendants. Method involves collecting living mature spermatozoa, making spermatozoa suspension in special purpose physiological medium, freezing the spermatozoa suspension for producing frozen spermatozoa, drying the frozen spermatozoa or spermatozoa heads in vacuum to humidity level of 1%, making rehydration of spermatozoa or spermatozoa heads with injured membrane and selecting those retaining nucleus of genetic integrity. The selected spermatozoa or spermatozoa heads are used for fertilizing isolated oocytes with living descendants being produced.
EFFECT: enhanced effectiveness in producing living descendants.
39 cl, 5 dwg, 1 tbl