Method for dentofacial tissue repair

FIELD: medicine.

SUBSTANCE: on completion of the antibacterial course, sodium hyaluronate in the form of a gel is single administered by means of a syringe with a needle of 2 cm long or more under an oral mucosa along a transitory fold into the points within projections of apexes of dental roots 1.4, 2.4, 3.6, 4.6 and into a point on the mid-line of projections of apexes of anterior roots of upper and lower jaw respectively. The needle is brought under the mucosa on the lower jaw by transecting the projections of an alveolar counterfort line. On the upper jaw - by transecting the projections of the upper counterfort line. While removing the needle from the mucosa, the syringe content is squeezed out. Sodium hyaluronate is administered into both jaws in an amount of 3.0 to 4.4 ml.

EFFECT: method enables increasing the jaw bone density within counterforts, as well as soft and bone tissues of periodontium.

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The invention relates to medicine, operative dentistry and can be used in complex treatment of inflammatory-destructive diseases of dental system to restore the soft and bone tissues of periodontal and bone tissues of the jaws, in particular, can be used for tissue repair dentition patients with contraindications to surgical treatment.

Periodontal disease, as a serious illness, the prevalence ranked fourth among non-communicable diseases, yielding pathology of the cardiovascular system, cancer and diabetes. Chronic generalized periodontitis is one of the most common pathological conditions of the periodontium. Chronic periodontitis is characterized by a progressive course with bone resorption of the alveolar bone, the death of the retaining apparatus of the tooth and loss (deletion) of the latter. All this leads to the degeneration of the tissues of the dentition. Most often, to repair damaged tissues of the dentition using a surgical method in which directly into the tissue of the periodontal lay stimulator of tissue regeneration of periodontal. However, after surgery remain large wound surface, which can PR�lead to additional complications. In addition, not every patient surgical treatment. In this case, the regeneration of hard and soft periodontal tissues is administered by injection directly into the soft tissues of the dentition.

A method of tissue repair dental system, which restores the bone tissue of the periodontium (RF patent No. 2265445, A61K 35/32, A61K 35/48, A61P 1/02, 10.12.2005). In accordance with the method used biotransplant, which is a suspension chondroprotective of human cells comprising a culture of genetically unmodified chondroprotective of human cells containing aggrecan and II collagen expressing cells of at least 80%, obtained from hyaline cartilage tissue of human embryos the 1-2nd trimester of pregnancy and selectively propagated in culture conditions. Biotransplant in suspension injected intraligamentary with the lateral and medial sides of each tooth and to paro subperiosteal dental plots the upper and lower jaws.

The method provides targeted physiological bone regeneration of the alveolar processes in the adult organism in the presence of inflammatory diseases of periodontal tissues.

A method of tissue repair dental system, whereby vosstanovit�Ute periodontal tissues. In accordance with the method used biomaterial, namely, a strain of diploid lung cells of the human embryo LAC-4 (81), which is administered by injection method in the region of the transition folds of the upper and lower jaws in the form of cell suspension course of 5-7 treatments 1 to 3 times per week at a dose on one injection of 500 to 700 thousand to 1 million cells, depending on the age (RF patent No. 2293566, A61K 35/54, A61P 1/02, 20.02.2007).

As a result of use of the known method the analysis of the indicator of the optical density of bone tissue of alveolar process with baseline tenzometricheskie figure to 100.e. showed an increase of 80% from baseline, and at an initial optical density at 100-200.e. increased by 60%.

The closest to the proposed is a method of tissue repair dental system, whereby for periodontal tissue repair using an injectable form of the introduction of platelet rich plasma in periodontal tissue. The plasma, after the course of antibiotic therapy, administered once under the mucous membrane of the oral cavity in the region of the transition folds at the rate of 0.3 ml per one tooth segment consisting of 3 teeth. The amount of plasma ranged from 2.7 to 3.0 ml and depended on the individual size of the jaw. (Nasibullina Camille Pautova, "Rehabilitation of periodontal tissues during orthodontic�ical treatment injectable form of autoplasma" // the dissertation on competition of a scientific degree of candidate of medical Sciences; Moscow, 2011).

Platelet-rich plasma is prepared by centrifugation from the blood taken from the cubital vein. After a year of using the value of the increase in mineral density of bone tissue alveolares process in the group with generalized periodontitis was 18%.

Common to all revealed during a patent search and analogues closest to the proposed regeneration of periodontal tissues is the complexity of manufacturing stimulator of regeneration of periodontal tissue, which complicates the implementation of the known methods.

In addition, the analysis of the above analogues and the closest to the proposed shows that the known methods have a positive impact on the regeneration of tissues of the dentition only in the area of the alveolar ridge. Thus, in relation to the closest to the proposed method, which uses injection in periodontal tissues platelet rich plasma, showed that increasing the density of bone tissue of the alveolar process, after appropriate treatment, more pronounced in the upper part of the alveolar bone and to a lesser extent affect the base part (Nasibullina Camille Pautova "rehabilitation of periodontal tissues at the stage of orthodontic treatment injectable form auto�of lazmi". The dissertation on competition of a scientific degree of candidate of medical Sciences, Moscow 2011).

At the same time, we know from literature that occur in the alveolar bone involutive processes negatively apply to the body of the maxillary bone (E. Y. Simanovsky, A. N. Lovikov, V. M. Twere, Yu nashin "Biomechanical description of the functions of the masticatory apparatus in normal and various pathological processes". Russian journal of biomechanics, vol. 8, No. 4, 2004, pp. 15-26). The ways identified in the patent search counterparts and are the closest to the proposed, only restore periodontal tissues, which prevents their use in complex treatment of inflammatory-destructive diseases of the dentition as a whole, namely: to restore the soft and bone tissues of periodontal and bone tissues of the jaws.

The present invention solves the problem of providing a method of tissue repair dental system, the implementation of which ensures the achievement of the technical result consists in the integrated treatment of inflammatory-destructive diseases of the dentition as a whole, namely: to restore the soft and bone tissues of periodontal and bone tissues of the jaws, and also to simplify the method.

The essence of the claimed invent the�tion is a method of tissue repair dental system, whereby patients underwent antibiotic therapy, what is new is that after the course of antibiotic therapy once injected sodium hyaluronate in the form of a gel syringe with needle, with a length of 2 cm, under the mucous membrane of the mouth on transition the crease points in the projection of the root apex of the teeth 1.4, 2.4, 3.6, 4.6 and at a point on the midline region of the projections of the apexes of roots of front teeth upper and lower jaws, respectively, while the lower jaw needle, is carried out under the mucous membrane, intersecting projection lines the alveolar buttress, and on the upper jaw - intersecting projection lines of the buttresses of the upper jaw, then, with the gradual removal of the needle from the mucous membrane, squeeze the contents of the syringe, at a flow rate of sodium hyaluronate on both jaws in the range from 3.0 to 4.4 ml.

The claimed technical result is achieved as follows.

The essential features of the invention: "Method of tissue repair dental system, whereby patients underwent antibiotic therapy, ..." are an integral part of the inventive method and provide its industrial feasibility, and, consequently, ensure the achievement of the claimed technical result�TA.

Sodium hyaluronate (Sodium Added). The sodium salt of hyaluronic acid - one of the most popular moisturizer and humectant components over long term. Get a biotechnological method (INTERNET, sodium hyaluronate).

Sodium hyaluronate (Gilan) - polysaccharide identical to the human. Used in rheumatology (ostenil, synvisc, fermathron) in arthritis of large joints and ophthalmology (healon) for eye operations.

Active substance: hyaluronic acid. Sodium hyaluronate (Gilan), biological analogue of hyaluronan is a polysaccharide, composed of repeating disaccharide links N-acetylglucosamine and sodium glucuronate. Has high molecular weight. A 1% solution of sodium hyaluronate is a hydrated gel. Sodium hyaluronate is used injections.

It is known the use of sodium hyaluronate in the form of a sterile viscoelastic implant for intra-articular injection into the synovial space in patients suffering from mild or moderate degree of osteoarthritis or osteoarthrosis; for therapy of the knee or any other synovial joints. In fact, is an analogue of human synovial fluid. On this basis, the introduction of sodium hyaluronate in the affected joint breaks a key link in the development of osteoarthritis, hap�dit affected by the cause the progression of osteoarthritis. therapeutic effects of hyaluronate sodium is associated with "filling viscosity, thereby improving the physiological and rheological status of the tissues of the affected joint. In addition, the effect of sodium Hyaluronate is based on the effect of "releaser" - immediately restores the ability of the joint to produce endogenous hyaluronan and thus returns it to a state of homeostasis, which persists for several months.

Applies to: when the pain and limited mobility caused by degenerative and traumatic changes of the knee, hip and other synovial joints; for the temporary replacement and replenishment of synovial fluid; in the treatment of patients, leading an active lifestyle and regularly intensive affected joint.

In addition, sodium hyaluronate is used in eye surgery. Sodium hyaluronate is a natural substance that is present in the extracellular connective tissue matrix in both animals and humans. Provides expansion of the anterior chamber of the eye, facilitates the separation of the eye tissue. Sodium hyaluronate reduces the risk of traumatic damage to the corneal endothelium and other tissues of the eye, ensures transparency of the visual fields.

In ophthalmology, it is used: expansion� anterior chamber or separating tissue, etc. (during operations on the anterior and posterior segments of the eye); a survey of the retina during and after surgery; laser therapy (auxiliary) (http//amt.allergist.ru/gialuronat_1.html, INTERNET).

The spectrum of therapeutic activity of hyaluronic acid is extremely wide: from wound healing (application WO 95/03786, MKI AC 31/725) and treatment of traumatic narrowing of the arteries (application EP 0661981, MKI AC 31/725) to cancer (application WO 95/30423, MKI AC 31/725) or stimulation of cell proliferation in the treatment of ulcers caused by pressure ulcers in diabetes or varicose veins of the lower leg (patent Sweden No. 501217, MKI AC 31/715), cosmetology - drug "Reneall", Russia, Moscow, Nagatinskaya str., building 3A, LLC "SLS".

The use of sodium hyaluronate in dentistry for tissue repair dental system by injecting under the mucous membrane of the mouth on transition the crease previously unknown and are proposed by the inventors.

Use of sodium hyaluronate in the form of a gel facilitating its preparation.

As experience has shown, year after single injections of sodium hyaluronate, made according to the inventive method, in patients with a diagnosis of rapidly progressive periodontitis were observed: an increase in bone density of the upper and lower jaws along the lines of the buttresses, the increase in tight�t bone on a sick gap visible alveolar bone; the growth of bone between the edge of the gums and radiographically determined bone tissue of the alveolar process, which ranges from 0.5 to 2.0 cm, thickening of the mucous membrane of the alveolar process, reducing the looseness of the gums, teeth strengthening ligaments to normal on the mobility of II-III degree, reducing the retraction of gingival oral and vestibular, thickening of turgor in the nasolabial triangle.

The use of sodium hyaluronate in dentistry for tissue repair dental system due to the following properties. From the literature it is known that hyaluronan - hyaluronic acid (HA), which is the current basis of sodium hyaluronate, in vivo has an important dual role. As the main material HA participates in the maintenance of homeostasis, lubrication of joints and plays a main role visco-elastic component of the extracellular matrix (ECM). As a signaling molecule, HA binds to specific proteins in the ECM, the cell surface and in the cell cytosol, thus participating in the stabilization of cartilage matrix, cell motility, the effect of the factors of growth, morphogenesis, and embryonic development and in inflammation ("Biomaterials out of hyaluronan for targeted drug delivery and wound healing", Glenn D. Prestwich, Li Luo, R. Kelly kirk�, Michael R. Zibell and Jeanselme Faculty of medical chemistry. The University of Utah, 30 South 2000 East, room 201, salt lake city, Utah, 84112-5820 USA Department of bioengineering. The University of Utah, 20 South 2030 East, room 506, salt lake city, Utah, 84112-9458 USA Department of neurobiology. Harvard medical school, 220 Longwood Avenue. Boston, mA 02115 USA Department of surgery. The University of Utah, 50 North medical highway, salt lake city, Utah 84132, USA.

It is known (see ibid.) that HA plays an important role in wound healing. This is due to the HA property, in accordance with which HA - rich matrix promotes infiltration of migrating cells in damaged tissue and provides an environment conducive to cell motility and proliferation. The decay products HA modulate the inflammatory response and stimulate angiogenesis. In addition, HA acts as a signaling molecule to provide cell motility, inflammation, and wound healing.

The inventive method uses sodium hyaluronate (Sodium Added), i.e. the sodium salt of hyaluronic acid, which further enhances regenerative properties, which is included in it HA. This is due to the following. It is known that one of the main mechanisms of power cell in the body - the flow of ions of trace elements and nutrients through the cell membrane by passive diffusion,�posredovanje vectors. Movement of substances against a concentration gradient is performed in the cell by active transport systems. Such a movement requires energy (active transport) or the use of active accelerator suction - membrane active substance. It is known that natural accelerator suction are sodium ions. Active transport of sodium ions across cell membranes is essential for the maintenance of homeostasis and ion asymmetry of cells and extracellular environments of all organs and tissues of humans and animals. He is responsible for such important life processes as absorption, secretion, growth, development and differentiation of cells in ontogeny, etc. (Tereshin, S. Y./Questions of balneology, 1999, No. 6; Antonov, V. F./ Lipids and ionic permeability of membranes, Moscow: Nauka, 1982; Tverdislov V. A. et al./ Physical mechanisms of functioning of biological membranes, Moscow: Moscow state University, 1987). Sodium ions are natural accelerator absorption of trace elements and nutrients through the cell membrane, adequate to the human body. Responsible for one of the most important life processes of cells - absorption, sodium ions, due to their content in the sodium hyaluronate, contributes to a more complete penetration into the cells of periodontal tissues therapeutic components deposited in the tissues of the periodontium� as the result of a course of drug therapy, thereby providing the possibility of implementing complex treatment of inflammatory-destructive diseases of the dentition as a whole. The result is increased efficiency for existence regenerating properties of HA, the active active ingredient in sodium hyaluronate.

In the inventive method, sodium hyaluronate) inject the introduction. And sodium hyaluronate is injected under the mucous membrane of the mouth on transition the crease points in the projection of the root apex of the teeth 1.4, 2.4, 3.6, 4.6 and at a point on the midline region of the projections of the apexes of roots of front teeth upper and lower jaws, respectively, while the lower jaw needle, is carried out under the mucous membrane, intersecting projection lines the alveolar buttress, and on the upper jaw - intersecting projection lines of the buttresses of the maxilla. As a result sodium hyaluronate fills all the defective space between the bone tissue of the alveolar process and gum.

In addition, as noted above, occur in the alveolar bone involutive processes apply to the body of the maxillary bone, thus reducing the severity of their relief (buttresses on the upper jaw, the shape of the articular process, the shape of the angle of the mandible). Note the change architectonics cancellous and, in General, exp�sions of its internal structure, the location of the trajectories of the spongy substance and the degree of their severity. The location of the trajectories cancellous reflects the direction of the chewing pressure. Lose the generating function activity dentoalveolar unit resulting in a progressive atrophy of the alveolar process and body of both upper and lower jaws (E. Y. Simanovsky, A. N. Lovikov, V. M. Twere, Yu nashin "Biomechanical description of the functions of the masticatory apparatus in normal and various pathological processes". Russian journal of biomechanics, vol. 8, No. 4, 2004, pp. 15-26).

The density of the facial bone structures of the skull varies because they have different load. Areas with denser bones are called lines of buttress (kgmuhelp.EN>content/118-cherep...cherepa-kontrforsy).

In General the lower jaw to allocate 2 buttress: alveolar and upward. Alveolar pointing up to the alveolar cells.

The lines of the buttresses of the upper jaw are directed upwards from alveranga bone of the upper jaw (human Anatomy, S. S., Mikhailov, A. V. Chukbar, A. G. Tsybulkin).

In the inventive method the proposed introduction sodium hyaluronate ("...in the lower jaw, the needle is carried out under the mucous membrane, intersecting projection lines the alveolar buttress, and on the upper jaw - intersecting projection lines of contro�owls of the upper jaw...") allows you to enter it as close as possible to the lines of the buttresses of the corresponding jaw. As a result, sodium hyaluronate, contributing to directional infiltration of migrating cells in damaged tissue and providing an environment conducive to cell motility and proliferation, creates conditions for the regeneration of bone tissue of the jaw.

The line of buttresses visible on computer tomography, which allows the physician to approach the process of introducing sodium hyaluronate individually for each patient. This increases the effectiveness of sodium hyaluronate.

As a result, in the inventive process, due to the above properties of sodium hyaluronate and the stated route of administration, sodium hyaluronate creates conditions for regeneration, as the damaged periodontal tissues and bone of the body of the respective jaw, i.e. creates conditions for tissue repair dental system as a whole.

In addition to the foregoing, in the claimed invention, sodium hyaluronate simultaneously performs the role of bioresorbable implant, as over time the hyaluronic acid breaks down under the influence of enzymes. As a result of injections of sodium hyaluronate fills all voids in the affected area of periodontal tissue, forming a kind of frame lost part of the supporting apparatus of the tooth in the region of its introduction, i.e. sodium hyaluronate concentrates�I in the diseased area of the periodontium, initiating the vital activity of the cells of periodontal tissues is directed, it is the affected area of the periodontium. In this case, being a bioresorbable implant, sodium hyaluronate over time disintegrates under the influence of enzymes, and in the field of administration remain the restored bone volume and soft periodontal tissues.

In severe forms of periodontitis, particularly in chronic generalized periodontitis, one of the stages of reconstruction of periodontal tissues is antibiotic therapy, which has a destructive effect on periodontal tissues.

We know from literature ("the Effect of oligosaccharides of hyaluronan on the expression of heat shock proteins". Chapin Hu, Tomomi ito, Akira Tawada, Hiroshi Maeda, Hiroko Yamanokuchi, Kyoko of Isahara, Yasuo of Uchiyama and Akira asari Corporation Seikagaku, Tateno 3-1253, Higashiyamato-Shi, Tokyo 207-0021, Japan Department of Cell biology and Anatomy, Medical school of Osaka University, Suita-Shi, Osaka, Japan the Presence.address: Department of ophthalmology. Medical school University of Aberdeen, Foresterhill, Aberdeen AV 2ZD, United Kingdom), to repair damaged cells in the body, when they are subjected to adverse environmental impacts, are called heat shock proteins (Hsp). It has been suggested that the oligosaccharides of ha, to fo�record through the degradation GK in the tissue, can suppress the damage of the cells by regulating the expression of Hsp72 (ibid.).

As claimed in the invention is sodium hyaluronate used in the form of injections, it is embedded in the periodontal tissues and thereby affects the expression of heat shock proteins, significantly reducing the destructive effect of antibacterial drugs on periodontal tissues. The result is improved conditions for regeneration of periodontal tissues.

Sodium hyaluronate is injected under the mucous membrane of the mouth on transition the crease of the lower or upper jaws, depending on the development of the disease, in points in the projection of the root apex of the teeth 1.4, 2.4, 3.6, 4.6 and at a point on the midline region of the projections of the apexes of roots of front teeth upper and lower jaws, respectively. This provides the safe administration of the drug, because in these points the introduction is missing the exits of major nerves and major blood vessels. At the same time, the introduction under the mucous membrane of the mouth on transition the crease and the proposed point of introduction ensure that the introduction of sodium hyaluronate in the vicinity of the place of action, and also uniform distribution of it in the introduction.

A single injection and consumption of sodium hyaluronate on both jaws in the range from 3.0 to 4.4 ml, yavl�are optimal and are obtained empirically.

The simplification of the method is ensured by the fact that sodium hyaluronate does not require additional preparation before use, as it comes in the form of a gel in a sterile package. For carrying out the method only needs to dial it in a syringe and inject under the mucous membrane of the oral cavity in the region of the transition folds of the lower or upper jaw (the drug "Reneall", "Reneall 300", Russia, Moscow, Nagatinskaya str., building 3A, LLC "SLS").

It follows from the foregoing that in the claimed invention, sodium hyaluronate, due to the proposed method of introduction, takes the form of a defect present in the periodontal tissues, and site-specific periodontal, forming a kind of frame missing periodontal tissues. In addition, since the introduction of sodium hyaluronate on the lower jaw needle, is carried out under the mucous membrane, intersecting projection lines the alveolar buttress, and on the upper jaw - crossing lines of the buttresses of the upper jaw, simultaneously it stimulates the regeneration of bone tissue of the jaw body. As a result in the claimed invention, sodium hyaluronate independently directionally stimulates the regeneration of tissues of the dentition.

Thus, it follows from the foregoing that the proposed method of tissue repair of dentition during the implementation provides d�achievement of the technical result namely, in the complex treatment of inflammatory-destructive diseases of the dentition as a whole, namely: to restore the soft and bone tissues of periodontal and bone tissues of the jaws, and also to simplify the method by injecting sodium hyaluronate into finished form.

Fig.1 shows a computed tomography of the patient rapidly progressive periodontitis before treatment, the results of measuring bone density in the area allergo the processes of the lower jaw, as well as the density of the buttresses of the upper and lower jaw before treatment, patient b; Fig.2 - computed tomography of the patient rapidly progressive periodontitis a year after treatment, the results of measuring the density of bone tissue in the alveolar bone of the mandible, as well as the density of the buttresses of the upper and lower jaw after treatment, patient b; Fig.3 - computed tomography of the patient rapidly progressive periodontitis, a three-dimensional image of the upper and lower jaws of the patient to treatment, patient b; Fig.4 - computed tomography of the patient rapidly progressive periodontitis a year after treatment, three-dimensional image of the upper and lower jaws of the patient one year after treatment, patient b; Fig.5 - computed tomography of the patient's bistrobar�esirous periodontitis before treatment, the results of measuring the density of bone tissue in the alveolar bone of the mandible, as well as the density of the buttresses of the upper and lower jaw before treatment, patient T.; Fig.6 - computed tomography of the patient rapidly progressive periodontitis a year after treatment, the results of measuring the density of bone tissue in the alveolar bone of the mandible, as well as the density of the buttresses of the upper and lower jaw after treatment, patient T.; Fig.7 computed tomography of the patient rapidly progressive periodontitis before treatment, three-dimensional image of the upper and lower jaws of the patient to treatment, patient T.; Fig.8 - computed tomography of the patient rapidly progressive periodontitis a year after treatment, three-dimensional image of the upper and lower jaws of the patient one year after treatment, the patient T. in Fig.9 is a photograph of the oral cavity of the patient prior to the treatment of rapidly progressive periodontitis, patient T.; Fig.10 is a photograph of the oral cavity of the patient one year after treatment of rapidly progressive periodontitis, patient T.; Fig.11 - computed tomography of the patient rapidly progressive periodontitis before treatment, the results of measuring the density of bone tissue in the alveolar bone of the upper jaw, and the density of the buttresses of the upper and lower jaw before treatment,the patient Ya. Fig.12 - computed tomography of the patient rapidly progressive periodontitis a year after treatment, the results of measuring the density of bone tissue in the alveolar bone of the upper jaw, and the density of the buttresses of the upper and lower jaw in a year after treatment, the patient Y.; Fig.13 - CT scan of the patient rapidly progressive periodontitis before treatment, three-dimensional image of the upper and lower jaws of the patient before treatment, the patient Y.; Fig.14 - CT scan of the patient rapidly progressive periodontitis a year after treatment, three-dimensional image of the upper and lower jaws of the patient one year after treatment. Patient Y.; Fig.15 is a photograph of the oral cavity of the patient prior to the treatment of rapidly progressive periodontitis, the patient Y.; Fig.16 is a photograph of the oral cavity of the patient one year after treatment of rapidly progressive periodontitis, the patient Y.

The claimed method of tissue repair of the dentition is as follows. The patient previously conducted antibacterial therapy, including: antibacterial therapy aimed at elimination parodontopathogenic microflora; anti-inflammatory therapy aimed at relieving inflammation and edema in the periodontal tissues; vitamin therapy.

After antibacterial� therapy once injected sodium hyaluronate in the form of a gel syringe with needle, with a length of 2 cm, under the mucous membrane of the mouth on transition the crease points in the projection of the root apex of the teeth 1.4, 2.4, 3.6, 4.6 and at a point on the midline region of the projections of the apexes of roots of front teeth upper and lower jaws, respectively. While the lower jaw needle, is carried out under the mucous membrane, intersecting projection lines the alveolar buttress, and on the upper jaw - intersecting projection lines of the buttresses of the maxilla. Then, with the gradual removal of the needle from the mucous membrane, squeeze the contents of the syringe. While sodium hyaluronate is injected at a flow rate of sodium hyaluronate on both jaws in the range from 3.0 to 4.4 ml.

The quantity of injected sodium hyaluronate depends on the severity of the process and the anatomical features of the patient.

The site of injection, it is desirable to anesthetize. After the manipulation of the injection press with a cotton swab.

The results of the reconstruction of periodontal tissues was monitored through the year by comparison with the original data.

In the course of work we examined 31 patients.

In the main group of 14 people appointed by sodium hyaluronate.

A comparison group consisted of 17 people, where sodium hyaluronate not appointed.

No. groupName groups� Main subgroupSubgroup comparisonsTotal
1Rapidly progressive Periodontitis To 05.4325
2CGP in the acute stage To 05.3101525
3CGP on the background somatic pathology101
Total141731

Age and gender of patients: women from 25 to 50 years, men from 25 to 60 years (in women after 50 years through menopause and, as a consequence, the development of postmenopausal osteoporosis, in men after 60 years, begins a pronounced reduction in peak bone mass).

All patients were administered a composition of drugs, including antibacterial therapy:

antibiotic Sumamed (the active ingredient Azitromicin) 500 mg (1 per day 3 per day half an hour before meals);

- Licopid (immuno�RR) (1 mg, 2 times a day half an hour before meals, dissolve in the mouth 10-14 days);

- Wobenzym - 2 tablets 3 times a day before meals 20-30 days;

- Saddique Forte - 1 tablet 1 time a day at night before bedtime for 30 days;

- Biosporin 2 doses morning and evening half an hour before meals for 10 days after taking the antibiotic.

The survey included a clarification of the complaints, history of present illness and life; recent or concomitant diseases; heredity; occupational exposures; the duration of the course of the disease, frequency of exacerbations, duration of periods of remission; treatment carried out earlier its effectiveness; compliance with the rules of hygiene. In addition, I removed the casts, diagnostic cast models, performed protectorate, taking pictures of patients before and after treatment. If necessary, appoint additional methods of examination of internal organs and consultations with relevant experts.

Conducted an external examination of the maxillofacial region, determining the color of the skin, presence of deformities, asymmetry, scarring. Using palpation revealed the state of the regional lymph nodes (submandibular, parotid, cervical). During examination of the oral cavity was evaluated by the depth of the vestibule of the oral cavity, especially the attachment of the frenulum. Also noted the presence of anomalies of the teeth, dentition, occlusion dental �poisons; carious and non-carious lesions of teeth, the quality of the seals (especially on contact surfaces and in the cervical area) and dental prostheses, the presence of soft and hard dental deposits.

The level of oral health and the condition of periodontal tissues was evaluated using hygienic indexes: green-Vermillion index of plaque (ZN) by Quigley u Hein; periodontal indexes: periodontal index (PI) (Russel A. Z.), bleeding index (IR) (N. Loe), papillare-marginal-alveolar index (PMA) (S. Parma), index Ramfjord (Ramfjorde), index Fuchs.

Tooth mobility was determined on a scale Miller (Miller) modification Flasar (Fleszar). Registration indexes was carried out in all groups of patients in terms of: 1st visit, 2 weeks, 3, 6, 12 months.

Depth clinical pockets were measured from four sides of the calibration tooth periodontal probe (D=0.5 mm). Most deep pocket, found on the surface, determined the final assessment of the study (Van der Velden).

Measurement of bone density was performed using computed tomography and measurement scales bone density in units of Hausfeld between two points. Density measurement was performed in a region of interest in three planes (axial, sagittal and coronal). Bone density was measured at the time of treatment and JV�STN year after treatment.

The source is sodium hyaluronate used as stimulator of regeneration of periodontal tissues, used drugs "Reneall", "Reneall 300". Drugs are medical devices and are used in cosmetology for the correction of deep wrinkles, anatomical defects and to fill the shortage of the volume of tissues (lacrimal groove, podsolevy area, etc.) Preparations are sterile, non-pyrogenic, bright, clear, viscoelastic gel; the current component is the sodium salt of hyaluronic acid. Commercially available packaged in a disposable syringe.

Example 1. Patient B., aged 57 appealed to maprotiline dental clinic UGMA, as disturbed tooth mobility, fan-shaped divergence of the teeth, bleeding gums when brushing your teeth, halitosis. Seven years ago the patient was prescribed a course of antibiotic therapy after professional oral hygiene. Annually supportive periodontal treatment. In the last two years watching bleeding of the gums when the reading of the teeth.

During the inspection the General condition of the patient is satisfactory. The face of the patient symmetrical, regional lymph nodes were not palpable. The chewing function is impaired. Shallow vestibule of the oral cavity. Strands in the region of the vestibule of the oral cavity. �of sticky upper lip, tongue, lower lip middle. The mucous membrane of the oral cavity: lips, cheeks, tongue, bright pink, shiny, moist without pathological entities. The mucous membrane of the alveolar processes of the upper and lower jaws, bright-red, visible supra - and subgingival plaque. The depth of periodontal pockets 6-7 mm in the Central group of teeth.

Index Russell (PI)=6,5. CT scan on the unit Planmeca.

Radiologically determined by destructive throughout the length of the alveolar bone with signs of inflammatory activity - resorption of interalveolar septa teeth more than one-half its length.

Diagnosis: Chronic generalized severe periodontitis. Partial loss of teeth. Shallow vestibule of the oral cavity, a heavyweight in the field of the oral vestibule. The deformation of the occlusal surface.

Picked up complex of pharmacological agents, including antibacterial and immunocorrective and enzymatic therapy. Loose teeth were deportirovany and siniawan. During the year the patient was performed twice professional oral hygiene. Recommended rational prosthesis.

The patient after antibiotic therapy once introduced sodium hyaluronate in the form of a gel syringe with needle, with a length of 2 cm, p�d the mucous membrane of the mouth on transition the crease points in the projection of the root apex of the teeth 1.4, 2.4, 3.6, 4.6 and at a point on the midline region of the projections of the apexes of roots of front teeth upper and lower jaws, respectively. While the lower jaw needle was performed under the mucous membrane, intersecting projection lines the alveolar buttress, and on the upper jaw - intersecting projection lines of the buttresses of the maxilla. Then, with the gradual removal of the needle from the mucous membrane, squeezed the contents of the syringe. When sodium hyaluronate was injected at a flow rate of sodium hyaluronate on both jaws, in this case in the amount of 4.0 ml.

Controlled the density of the buttresses and the alveolar process of the upper jaw of the patient before and after treatment by computed tomography in the coronary slice. The results are summarized in the table.

Bone density in units of Hausfeld before treatmentThe bone density in units of Hausfeld after treatment
The buttress of the upper jaw
Coronary slice
The average density value473566

The maximum density9861064
Minimum density-294-58
The buttress of the mandible
Coronary slice
The average density value8631059
The maximum density11011651
Minimum density353146
The alveolar process of the maxilla. Coronary slice.
The average density value271619
The maximum density9041046
Minimum density-314 120
The alveolar process of the maxilla. Axial slice.
The average density value205537
The maximum density5421008
Minimum density-18052
The alveolar process of the maxilla. Surgically slice.
The average density value214600
The maximum density6001134
Minimum density-260261

Comparative analysis of the results of measurements of the density of alveolar bone and buttresses of the upper and lower jaws of the patient before and one year after treatment shows a significant increase in bone density (Fig.1, Fig.2). Improvement of alveolar tissue atractaspididae and the results of comparing three-dimensional images of the upper and lower jaws of the patient before (Fig.3) and a year after (Fig.4) treatment. Fig.4 clearly shows the filling of bone defects in the alveolar bone. A year later, the patient reported improvement in the oral cavity: disappeared bleeding gums, decreased tooth mobility. The mucous membrane of the alveolar process of the pale pink color, close-lying teeth.

Example 2. Patient T., 22 years old was sent to maprotiline dental clinic UGMA, as treatment in the periodontist at the place of residence (Pervouralsk) has not resulted in the positive dynamics of the disease. The patient is concerned about a sharp tooth mobility, pain when eating, recurrent inflammation of the gums.

The patient had spontaneous bleeding of the gums, mobility of teeth, bad breath. In a patient with history of chronic and hereditary cyclic neutropenia. Is at the dispensary hematologist at the place of residence. The patient from an early age suffered from inflammatory processes of different localization. Suffered a sepsis of three years ago. Has meatwad from all vaccinations since birth. History monthly cold sores in the mouth - in the bottom of the oral cavity and in the area of the mucous membrane of the cheeks.

During the inspection of the General condition of the patient satisfactory. The patient's face are symmetrical, regional lymph nodes were not palpable. Puncturevine violated. The frenulum of the upper lip, tongue, lower lip middle. The eve of the oral cavity of medium depth, the mucous membrane of the oral cavity: lips, cheeks, tongue, bright pink. A blood test er. 3,5·10'2/l, Hb 113 g/l, L. 3,8·10/l, E. Oh, p., S. lymphs. 42%, Mont. 42%; ESR 53 mm/h.

In the mouth redness and swelling of the mucous membrane of the alveolar process. Papillomatous growth of interdental gingival papilla. You can see above - and subgingival plaque. Supragingival dark yellow, subgingival dark brown color.

Tooth mobility II(18, 17, 16, 15, 14, 13, 21, 22, 24, 48, 47, 46, 38, 34, 32) and III (28, 37) degrees on a scale Miller, modification Fleser, sustainable(23, 33, 43, 44). Depth paro dental pockets 5-6-7 see Index Russell (PI)=6,5.

CT scan on the unit Planmeca. Radiologically determined by destructive throughout the length of the alveolar bone with signs of inflammatory activity - resorption of interalveolar septa teeth more than one-half its length.

Diagnosis: prudently syndrome (periodontal diseases) on the background of cyclic neutropenia. Partial loss of teeth, deformation of the occlusal surface. The lack of abrasion of the bumps of teeth.

Together with the hematologist selected complex pharmacological agents, including antibacterial, antiviral, immune-modifying and �fermentativnuyu therapy further - eubiotic. The patient received pre-professional oral hygiene, training and selection of new funds to care for the oral cavity. Locally designated Acepta balm and gel for gums.

It is recommended to consult a dentist podiatrist for the purpose of rational prosthetics and consulting a dental surgeon to remove the teeth with mobility III.

Particular emphasis was placed on the need for regular oral health as well as the rapid elimination of exacerbation of the process contributes to the preservation of bone component paro complex dental tissues.

After performing drug therapy the patient is entered in accordance with the method claimed sodium hyaluronate.

Computer tomography was done again in a year.

Determined the density of bone tissue buttress the mandible and the alveolar process of the mandible, maxilla before and one year after treatment. The measurement results are summarized in the table below.

Bone density in units of Hausfeld before treatmentBone density in units of Hausfeld after treatment
The buttress of the lower jaw. Coronary slice.
The average density value717154
The maximum density9861548
Minimum density114504
The alveolar process of the maxilla. Coronary slice.

The average density value452512
The maximum density1007945
Minimum density-17102
The alveolar process of the maxilla Axial slice
The average density value499 557
The maximum density9241209
Minimum density12097
The alveolar process of the maxilla Sagittal slice
The average density value336612
The maximum density10171294
Minimum density-176-20

Comparative analysis of the results of measurements of bone density buttress the mandible and the alveolar processes of the jaws of the patient before and one year after treatment shows a significant increase in bone density (Fig.5, Fig.6). This is confirmed by a three-dimensional image of the jaws before and one year after treatment (Fig.7 and Fig.8).

In addition, a comparative analysis of three-dimensional images of the jaws before and one year after treatment (Fig.7, Fig.8) shows that before treatment the patient had very low bone density of the buttresses of the upper jaw, as in Fig.7 the upper buttresses� jaw visible. A year after treatment Fig.8 visible left and right buttresses of the upper jaw, which demonstrates the increased density of the bone of the upper jaw in the line of buttresses.

A year later, the patient noted improvement in General condition: in the last year of remission cyclic neutropenia reaches 3-4 months passes without increasing the temperature. The condition of the oral cavity also improved: the mucosa is pale pink, no signs of hyperemia and edema of the mucous membrane of the alveolar process (Fig.9 and Fig.10).

Example 3. Patient Y., 37 years old, came in monopropylene dental clinic UGMA, as disturbed tooth mobility, displacement of the mandible to the left, bleeding gums when brushing your teeth. Earlier it was observed in the periodontist in a residence in the town of Asbestos. Watches bleeding gums when reading of the teeth more than 3 years.

During the inspection the General condition of the patient is satisfactory. The face of the patient symmetrical, regional lymph nodes were not palpable. Chewing function is not impaired. Shallow vestibule of the oral cavity. Strands in the region of the vestibule of the oral cavity. The frenulum of the upper lip, tongue, lower lip middle. The mucous membrane of the oral cavity: lips, cheeks, tongue, bright pink, shiny, moist without pathological entities. The mucous membrane of the alveolar processes of the upper and lower jaws bright�red color you can see above - and subgingival plaque. The depth of periodontal pockets 6-7 mm in distal teeth of the upper jaw on the left. Index Russell (PI)=6,5.

CT scan on the unit Planmeca. Radiologically determined by destructive throughout the length of the alveolar bone with signs of inflammatory activity - resorption of interalveolar septa teeth more than one-half its length.

Diagnosis: chronic generalized severe periodontitis. Partial loss of teeth. Shallow vestibule of the oral cavity, a heavyweight in the field of the oral vestibule. The deformation of the occlusal surface.

Picked up complex of pharmacological agents, including antibacterial and immunocorrective, vitamin therapy and enzyme therapy. Loose teeth were deportirovany and siniawan. During the year the patient was performed twice professional oral hygiene.

After performing drug therapy the patient is entered in accordance with the method claimed sodium hyaluronate.

Computer tomography was done again in a year.

Determined bone density buttresses of the lower and upper jaw and alveolar process of maxilla before and one year after treatment. The measurement results are summarized in the table below./p>

Bone density in units of Hausfeld before treatmentBone density in units of Hausfeld after treatment
The buttress of the upper jaw Coronary slice
The average density value247750
The maximum density7541118
Minimum density-311-12
The buttress of the mandible coronal slice
The average density value8791277
The maximum density12221583
Minimum density107733
The alveolar process of the maxilla. Coronary slice.
The average density value-14267
The maximum density13612
Minimum density-314-257
The alveolar process of the maxilla. Axial slice.
The average density value-216-38
The maximum density153504
Minimum density-446-285
The alveolar process of the maxilla. The sagittal slice.
The average density value-12415

The maximum density16698
Minimum density-310-289

Comparative analysis of the results of measurements of bone density of the alveolar bone of the upper jaw and buttresses of the upper and lower jaws of the patient before and one year after treatment shows a significant increase in bone density (Fig.11, Fig.12). Improving the condition of bone tissue of alveolar process and jaw in the area of the buttresses is confirmed by the results of comparing three-dimensional images of the upper and lower jaws of the patient before (Fig.13) and a year after (Fig.14) treatment. Fig.14 clearly shows the filling of bone defects in the alveolar bone. In addition, the right buttress of the upper jaw before treatment not visible, which indicates a low density of a bone of the upper jaw in the area of the buttress (Fig.13). A year after treatment Fig.14 visible to the left and right buttresses of the maxilla.

The condition of the oral cavity before treatment: the mucous membrane of the alveolar processes of the upper and lower jaws, bright-red, visible supra - and subgingival plaque, tooth neck covers loose, (Fig.15). A year later, the patient reported improvement in the oral cavity: disappeared bleeding gums, decreased tooth mobility. The mucous membrane of the alveolar process of the pale pink color, fits closely to the neck� teeth (Fig.16). Disappeared bleeding gums, decreased tooth mobility.

Is recommended to consult a dental surgeon for the purpose of conducting reconstructive operations in the area of the vestibule of the oral cavity.

The above examples of the method illustrate effective recovery method claimed tissues of the dentition in patients with chronic generalized severe periodontitis, and one of them (Example 2) periodontal syndrome (periodontal diseases) on the background of cyclic neutropenia, partial loss of teeth, deformation of the occlusal surface, the lack of abrasion of the tubercles of the teeth. Examples of the claimed invention is confirmed that the introduction in accordance with the inventive method after the end of drug therapy sodium hyaluronate provides a significant increase in bone density not only of the alveolar processes of the upper and lower jaw, but the jaw bones, and in the buttresses. In this case, as shown by computed tomography, bone density increases directly: so in the alveolar bone the bone density is increased in the place of greatest destruction of bone tissue. In addition, the results of a comparative analysis of three-dimensional images of the jaws before and one year after treatment clearly de�will Instituut the growth of alveolar bone and in the place of greatest bone destruction

At the same time due to the influence of sodium hyaluronate on the increased activity of heat shock protein, reduced negative effects from the effects of antibacterial drugs on periodontal tissues, thereby contributing to their recovery.

Thus, the use of the claimed method in the complex treatment of inflammatory-destructive diseases of dental system provides restoration of soft and bone tissues of periodontal and bone tissues of the jaws, i.e. ensures the recovery of the tissues of the dentition as a whole.

Method of tissue repair dental system, whereby patients underwent antibiotic therapy, characterized in that after the course of antibiotic therapy once injected sodium hyaluronate in the form of a gel syringe with needle, with a length of 2 cm, under the mucous membrane of the mouth on transition the crease points in the projection of the root apex of the teeth 1.4, 2.4, 3.6, 4.6 and at a point on the midline region of the projections of the apexes of roots of front teeth upper and lower jaws, respectively, while in the mandible the needle is carried out under the mucous membrane, intersecting projection lines the alveolar buttress, and in the upper jaw - intersecting projection lines of the buttresses of the upper cells�and, then, with the gradual removal of the needle from the mucous membrane, squeeze the contents of the syringe at a flow rate of sodium hyaluronate on both jaws in the range from 3.0 to 4.4 ml.



 

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12 cl, 7 dwg, 3 tbl, 1 ex

FIELD: chemistry.

SUBSTANCE: invention relates to a novel compound - N,O-(2,3-dihydroxypropyl)chitosanyl-borate, having formula , where m=500-3000.

EFFECT: compound has antibacterial, immunomodulating and antitoxic effect.

1 tbl, 6 ex

FIELD: chemistry.

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2 tbl, 1 dwg

FIELD: medicine.

SUBSTANCE: there are described compositions containing hyaluronic acid with a low degree of modification of functional groups, and mixtures prepared by a controlled reaction of this slightly modified hyaluronic acid with applicable difunctional or polyfunctional cross-linking agents. The compositions possess the low anti-inflammatory properties in injection in vivo and can be used as medical devices, biomedical adhesives and sealing matters.

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49 cl, 14 dwg, 24 tbl, 45 ex

FIELD: biotechnology.

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20 cl, 18 ex

FIELD: chemistry.

SUBSTANCE: invention relates to natural polysaccharide polymers and can be used in medicine. The obtained water-soluble bioactive nanocomposite includes a melanin compound-modified hyaluronic acid salt as a matrix and gold nanoparticles as filler. The method includes chemical reaction of solid-phase hyaluronic acid powder, a melanin compound, aurichlorohydric acid or a gold salt in conditions of simultaneous pressure action in the range of 50 to 1000 MPa and shearing deformation in a mechanochemical reactor at temperature of -18° to 110°C.

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4 cl, 18 ex

FIELD: chemistry.

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EFFECT: effective liposome composition stabiliser which can be obtained using a simple method.

8 cl, 3 tbl, 5 ex, 7 dwg

FIELD: chemistry.

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1 ex, 1 tbl

FIELD: biotechnologies.

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EFFECT: improvement of the method.

2 dwg, 1 tbl, 1 ex

FIELD: biotechnologies.

SUBSTANCE: production method of glucan-chitosan complex from yeast biomass of brewing waste involves mechanical and ultrasonic treatment of yeast biomass, destruction of proteins by treatment of the obtained suspension using alkali reagents with further extraction of a target product. As biomass, Saccharomyces living yeast is used. First, yeast is frozen to -15°C during 24 hours. After mechanical destruction, biomass is treated for 15 minutes at 20°C in an ultrasonic bath with frequency of an emitter of 35 kHz and voltage of 285 W. Biomass is acidified with chlorhydric acid till pH=5.5 and treated with ferment preparation in the amount of one pellet containing lipase - 3500 units of Ph.Eur., amylase - 4200 units of Ph.Eur. and protease - 250 units of Ph.Eur. per kilogramme of biomass in terms of dry substance; then, lipid components of yeast are removed. Fermentation is performed at t=20-29°C during 30-60 minutes. Destruction of proteins is performed at 55°C by means of a water bath during 60 minutes by treatment using 4% water solution of caustic soda at the ratio of yeast biomass and alkali, which is equal to 1:4. The medium is neutralised and hydrosol of glucan-chitosan complex is deposited by centrifugation during 10 minutes. The deposit is dried at t=55°C during 48 hours.

EFFECT: invention allows improving the quality of the obtained complex and its biological activity.

3 ex

FIELD: medicine.

SUBSTANCE: invention refers to dentistry and concerns treating chronic generalised periodontitis. Implementing the above method is ensured by a complex preparation in the form of an ointment of the following composition: Vaselin - lanolin base (1:1) - 88.5 g, 70% ipecac infusion - 5.0 g, 70% scholar tree - 5.0 g, Ecdysterone - 0.02 g; eucalyptus oil - 1.0 g. The oil is applied as a therapeutic periodontal applicate on a gingival surface following application anaesthesia with 10% lidocaine, chairside oral hygiene, drug-induced treatment of the gingival margin with 1% Iodinolum and separation of salivary glands in the oral cavity, once a day for 2 hours for 4 weeks; the patients are recommended not to eat or drink for two hours. The preparation composition particularly provides capillary-reinforcing, anti-inflammatory, reparative and anaesthetic action.

EFFECT: method of treating is easy to use, physiologically-friendly, and provides the complete recovery.

2 ex

FIELD: chemistry.

SUBSTANCE: invention relates to a compound of formula (I) : or a salt thereof, wherein R1 and R5 are independently selected from H, OH and alkoxy; R2-R4 and R6-R8 are independently selected from H, OH, F, Cl, Br and I; R9 and R10 are C2-C8 alkenyl; under the condition that at least one of R1, R5 and R7 is OH or alkoxy; at least one of R2-R4, R6 and R8 is F, Cl, Br or I; and R2 and R6 are Cl. The invention also relates to an antibacterial composition and treatment methods.

EFFECT: improved properties.

18 cl, 7 ex, 10 tbl

FIELD: medicine.

SUBSTANCE: agent for treating inflammatory periodontal and oral mucosal diseases associated with Helicobacter infection contains silicone glycerohydrogel of Si(C3H7O3)4·6C3H8O3·24H2O and bismuthate tripotassium dicitrate of formula [HOC(CH2COO)2COO]2K3Bi in the following proportions, wt %: bismuthate tripotassium dicitrate 1.0-2.5; silicone glycerohydrogel - the rest up to 100. The method of treating inflammatory periodontal and oral mucosal diseases consists in a combination of systemic standard anti-Helicobacter therapy and a local effect of the above agent on the involved region. Particularly, treating periodontitis is ensured by applying the agent on the gingival surface and introducing it into the gingival pockets once a day for 10 min; the therapeutic course makes 5-6 days. The oral mucosal diseases are treated by applying the agent 0.1 mm thick with a glass spatula on the involved oral mucosa 2 times a day; the therapeutic course is 12 days.

EFFECT: formulation of the agent provides achieving the high therapeutic effect; the dosage form is easy to be applied locally.

4 cl, 2 dwg, 1 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: method includes the preparation of a carious cavity, opening the tooth cavity, creation of an access to root canals, extension of their orifice. Removal of a decayed material from the root canals and their drug treatment are carried out. After that, performed are: wide opening of the apical tooth orifice, mechanical and drug removal of periapical pathological exudative formations in the focus of periapical inflammation through the root canal. Before filling the canal with a filling material the gel "Lamifaren" is introduced into the focus of periapical destruction. Such introduction is performed three times after a day under temporary stopping. The gel "Lamifaren" is introduced inside in a dose of 50 g 2 times per day for 30 days.

EFFECT: application of the invention accelerates bone tissue regeneration due to the active release of an active substance calcium alginate, provides stable remission due to local and systemic detoxifying effect.

2 ex

FIELD: medicine.

SUBSTANCE: treatment is two-staged; at the first stage, gingival pockets are rinsed with a stream of 1.5% hydrogen peroxide in a cannula delivered through the gingival pockets around the teeth. If observing the exposed furcation of premolars and molars, the cannula is brought under the root furcation. Thereafter, the gingival pockets are similarly rinsed with an aqueous tincture of garden sage herb for 3-5 days, then with an aqueous tincture of camomile blossom for 3-5 days, an aqueous tincture of plantain leaves daily for the following 3-5 days; each rinsing procedure is followed by applications of cotton drains impregnated with the respective aqueous herbal tincture introduced by the cannula on gingival mucous membranes for 1 hour; that is combined with prescribing oral baths with the respective aqueous herbal tincture introduced by the cannula daily 5-7 times a day. The second stage involves instillations of Normoflorin-B or Bifidumbacterin in the cannula delivered through the gingival pockets around the teeth, brought under the furcation if observing the exposed furcation of premolars and molars; the gingival mucous membranes are laid with cotton drains with the above preparation for 2 hours daily for 5-10 days.

EFFECT: method enables providing the higher clinical effectiveness.

2 cl, 1 ex

FIELD: medicine.

SUBSTANCE: at the first stage, after removing supra- and sub-gingival dental calculus and granulations, cleaning the teeth professionally, rinsing gingival pockets by stream infusions of 1.5% hydrogen peroxide in a cannula delivered through the gingival pockets around the teeth, brought under root furcation if observing the exposed furcation of premolars and molars; the preparation "Calsept" is administered within the frontal teeth for 5-7 days daily into the gingival pockets bringing the cannula similarly around the teeth, whereas the preparation "Calsept-Iodo" is administered within the distal teeth; the dentist recommends that the patient does not eat for 2 hours thereafter. The second stage involves administering a preparation containing bifidus bacteria, e.g. Normoflorin-B or Bifidumbacterin by instillations in a cannula delivered through the gingival pockets around the teeth, brought under the furcation if observing the exposed furcation of premolars and molars; gingival mucous membranes are laid with cotton drains with the preparation for 2 hours daily for 5-10 days.

EFFECT: using the method provides increasing the therapeutic effect ensured by the antibacterial effect, odontotropic and anaesthetic action, bone tissue recovery, action of the therapeutic preparation in difficult-to-access anatomic formations.

2 cl, 1 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to health-promoting compositions and methods for preparing them. A method for preparing the composition of non-living lactic acid bacilli, possessing an ability of specific binding to Streptococcus mutans, involves the following stages: heating a cell suspension of lactic acid bacillus or a mixture of lactic acid bacilli possessing an ability of specific binding to Streptococcus mutans from an initial temperature of less than 40°C to a pasteurisation temperature of 75 to 85°C with a temperature variation within the range of 0.5 to 2°C/min, keeping the heated suspension at a pasteurisation temperature of 20 to 40 minutes and cooling the suspension to a final temperature of less than 40°C within the range of 0.5 to 2°C/min. The specific binding the cell suspension of the lactic acid bacillus or the mixture of lactic acid bacilli to Streptococcus mutans is stable to heat treatment and/or resistant to proteases and/or calcium-dependent and/or is observed within the range of pH values falling within the range of 4.5 and 8.5, and/or in the saliva environment.

EFFECT: invention enables producing the agent preventing or delaying the caries lesion formation.

9 cl, 4 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: group of inventions refers to oral care compositions containing a basic amino acid or its salt. The presented oral care composition promoting the dentine defect closure in the oral cavity contains arginine in the free form or in the form of a salt, and an abrasive substance representing synthetic amorphous silica and containing small particle fractures making at least approximately 5% of total weight of the composition, wherein the particles of the small particle fracture having d50 from 3 to 4 mcm. What is also presented is a method of treating sensitive teeth in the oral cavity involving using the oral treatment with this composition, as well as using arginine as a part of the oral care composition and for producing a therapeutic agent, wherein the above composition and agent contain the above abrasive material containing the small particle fracture.

EFFECT: group of inventions provide the effective dentin defect closure in the patient's oral cavity.

8 cl, 1 tbl, 6 ex

FIELD: medicine.

SUBSTANCE: carious cavity is prepared. Then it is treated instrumentally and antiseptically. A hole of an apical canal of a causative tooth is expanded. Methylene blue is introduced into the cyst through a root canal at a depth of 7-10 min. The cyst envelope is exposed to a laser light generated by a diode laser through the root canal for 30-60 s by means of a needle light guide. The exposure is continued from the vestibular or oral surface for 60.0-120.0 s by means of a plate light guide of intensity 0.5-1 W, wave length 625-630 nm in a pulse mode. Before the root canals of the causative tooth is hermetically sealed, Collap-An gel with metrogil 1.0-1.5 ml is introduced into the cyst.

EFFECT: method enables provides increasing clinical effectiveness, avoiding the complications by removing the cyst envelope completely that enables restoring the bone defect structure completely.

2 ex

FIELD: medicine.

SUBSTANCE: agent for local therapy of recurrent oral aphthae accompanying dental stomatitis and periodontitis contains glycerine, kalanchoe juice and common nettle juice, glucosamine hydrochloride and dimethylsulphoxide, lavender, common thyme, garden sage and pepper mint essences, lime blossom and drinking water in a certain ratio.

EFFECT: agent possesses anti-inflammatory, antibacterial, wound healing, haemostatic, local anaesthetic, saliva thinning properties enabling its effective use for combined periodontal and oral mucosal pathologies in the form of dental stomatitis and periodontitis, including if observing deep periodontal pockets.

3 ex

Tooth paste // 2246931

FIELD: medicine, stomatology.

SUBSTANCE: the present innovation deals with preventing diseases and hygiene of mouth cavity. The suggested tooth paste contains chalk, sodium carboxymethylcellulose, glycerol and/or sodium laurylsulfate, vitamin additive, biologically active components of plant origin, functional additives and water, moreover, as vitamin additive it contains vitamin C, as biologically active components it contains lavender and eucalyptus oils and, additionally, it contains menthol. Components should be taken at a certain quantitative ratio. The suggested tooth paste has mint-plant odor with a scent of lavender and eucalyptus and refreshening taste by protecting teeth and parodontium tissues.

EFFECT: better sanitation and prophylactic action.

4 cl, 3 ex

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