Method of estimating osteointegration of porous wire materials in experiment

FIELD: medicine.

SUBSTANCE: method includes sampling bone blocks with a porous wire material to be analysed, fixation of the bone tissue with the porous wire material, decalcination, degreasing, dehydration, pouring the said tissue, preparation of cuts, staining and histomorphometry. Before or after the fixation of the bone tissue with the analysed porous wire material and before or after decalcination search and fixation of an edge wire element of the porous wire material in the bone block is carried out. Untwisting of the porous wire material from the bone block is performed by the method of pulling out the edge wire element. An analysis of macropreparations is carried out when pore spaces of the porous wire material are emptied. After that, the tissues are sampled from the emptying pore spaces, including the sampling and analysis of intra-pore fragments.

EFFECT: possibility of the qualitative and quantitative estimation of histomorphometric parameters of intra-pore tissues in the entire volume of an implant.

3 ex, 1 dwg

 

The invention relates to medicine, namely to experimental surgery, pathological anatomy, pathological physiology and can be used to evaluate the osseointegration of porous wire materials in experiment.

Various methods for the evaluation of osseointegration of porous wire materials in experiment.

Known way to assess the osseointegration of porous wire materials in the experiment, representing the deep etching of the bone blocks with the engraftment of the titanium implants. Produce preparation solution with the following ingredients: 40% R-R hydrofluoric acid 220 g; zinc, 100 g; ethylene glycol 800, Dipped in the prepared solution of the bone block with the engraftment of the titanium implants. The method allows to obtain the opportunity to study the processes of bone growth inside the porous implants [1].

The disadvantage of this method is the destruction of the chemical components of the solution of bone tissue, lack of credibility of research results.

Known way to assess the osseointegration of porous wire materials in the experiment, representing the fence at autopsy bone fragment material, separation of the bone blocks in the area of implantation, the fixation of the fragments in 10% formalin solution, dehydrated in vakuumpakkere, metallization of the samples for the study of the gold-palladium coating, scanning electron microscope [2].

The disadvantages of this method are the destruction of bone tissue by physical impact factors, insufficient validity of the research results, surface analysis removed macroscopic specimen, inability to hold histomorphometrical analysis of osseointegration in a depth of the studied material.

Known way to assess the osseointegration of porous wire materials in the experiment, representing the harvesting of bone blocks with the studied porous wire material, fixation in formalin solution, decalcomanie, degreasing, dehydration bone blocks with the studied porous wire material, fill in paraffin, manufacture of stripes on the border of the bone - implant, staining and histomorphometry [3].

The disadvantage of this method is the insufficient reliability of the results of the study, surface analysis removed macroscopic specimen, inability to hold histomorphometrical analysis of osseointegration in a depth of the studied material.

This method is taken as a prototype.

The aim of the invention is to ensure reliability of the results of the study of osseointegration, analysis macropreparations with the release of the pore spaces of a porous wire�of material, enabling, fence tissue released from the pore spaces of a porous wire material for histomorphometrical research, creating the opportunity for sampling and analyzing interstitial fragments porous wire material.

This object is achieved in that before or after fixation of the bone tissue with the studied porous wire material, and before or after decalcification of bone tissue of the studied porous wire material, perform and record boundary wire element porous wire material in the bone block; and then produce Raspletina porous wire material from a bone block method of drawing the boundary wire element porous wire material; analyze macropreparations with the release of the pore spaces of a porous wire material; producing the tissues released from the pore spaces of a porous wire material; producing the sampling and analysis of interstitial fragments of porous wire material.

The method is illustrated graphic material.

In Fig. 1 shows Raspletina porous wire material from a bone block method of drawing the boundary wire element porous wire material.

Method is used as the image�M.

In the experiment under General anesthesia produced euthanasia of the animal. Produce fence bone block with the studied porous wire material. Before or after fixation of the bone tissue with the studied porous wire material, and before or after decalcification of bone tissue of the studied porous wire material, perform and record boundary wire element porous wire material in the bone block. Produce Raspletina porous wire material from a bone block method of drawing the boundary wire element porous wire material. Analyze macropreparations with the release of the pore spaces of a porous wire material. Produce fence fabrics released from the pore spaces of a porous wire material. Produce sampling and analyzing interstitial fragments porous wire material. Produce a fill of bone tissue released from the pore spaces of a porous wire material. Make slices of bone tissue. Stained bone. Produce bone histomorphometry. Assess osseointegration of porous wire material with bone tissue.

In the proposed method opens the possibility of analyzing interstitial fragments porous wire mate�IALA, analysis macropreparations with the release of the pore spaces of a porous wire material, opens the possibility of biopsy from back-ordered pore spaces of a porous wire material. This method is not disturbed native bone tissue structure which integrated porous wire material. Raspletina porous wire material from a bone block method of drawing the boundary wire element porous wire material is high-energy and denaturing factor for bone tissue.

Achieve the result of the new method is confirmed by examples, produced on the basis of the Institute of experimental medicine and biotechnology, Samara state medical University.

The method presents the following examples.

Example 1. From the vivarium taken rat-male Wistar weighing 190 g., previously operated in the experiment with the installation in the region of the scapula porous wire material. At the stage of integration, under General anaesthesia (Zoletil - Rameter) is produced by animal euthanasia by intracardiac injection of a solution of thiopental sodium. Produce fence bone block with the studied porous wire material. To fixation of bone studied with porous wire material and to decalcification of cottoidei with the studied porous wire material, under physiological bath solution derived search and fixation of boundary wire element porous wire material in the bone block. Produced by Raspletina porous wire material from a bone block method of drawing the boundary wire element porous wire material. The analysis removed macroscopic specimen with the release of the pore spaces of a porous wire material. Derived tissue samples released from the pore spaces of a porous wire material. Produce sampling and analyzing interstitial fragments porous wire material.

Produced by fixation of bone studied with porous wire material formalin solution. Produced by decalcomanie bone tissue of the studied porous wire material in a solution of Trilon-B, degreasing, dehydration of bone tissue of the studied porous wire material. Produced fill the bone tissue. Slices with a thickness of 5 μm is made on a rotary microtome Sakura with subsequent staining with hematoxylin and eosin and pikrofuksin by van gieson. Held histomorphometry of bone tissue. Evaluated the osseointegration of porous wire material in the experiment.

Example 2. From the vivarium taken rat-male Wistar weighing 200 g, earlier operated in the experimental�the have their installation in the region of the scapula porous wire material. At the stage of integration, under General anaesthesia (Zoletil - Rameter) is produced by animal euthanasia by intracardiac injection of a solution of thiopental sodium. Produce fence bone block with the studied porous wire material. Produced by fixation of bone studied with porous wire material in formalin solution. After fixation of the bone tissue with the studied porous wire material and to decalcification of bone tissue of the studied porous wire material made finding and fixing the boundary wire element porous wire material in the bone block. Produced by Raspletina porous wire material from a bone block method of drawing the boundary wire element porous wire material. The analysis removed macroscopic specimen with the release of the pore spaces of a porous wire material. Derived tissue samples released from the pore spaces of a porous wire material. Produce sampling and analyzing interstitial fragments porous wire material. Produced by decalcomanie bone tissue of the studied porous wire material in a solution of Trilon-B, degreasing, dehydration of bone tissue of the studied porous wire material. Produced fill the bone tissue. Slices with a thickness of 5 MK� made on a rotary microtome Sakura with subsequent staining with hematoxylin and eosin and pikrofuksin by van gieson. Held histomorphometry of bone tissue. Evaluated the osseointegration of porous wire material in the experiment.

Example 3. From the vivarium taken rat female Wistar weighing 180 g., previously operated in the experiment with the installation in the region of the scapula porous wire material. At the stage of integration, under General anaesthesia (Zoletil - Rameter) is produced by animal euthanasia by intracardiac injection of a solution of thiopental sodium. Produce fence bone block with the studied porous wire material. Produced by fixation of bone studied with porous wire material in formalin solution. Produced by decalcomanie bone tissue of the studied porous wire material in a solution of Trilon-B, degreasing, dehydration of bone tissue of the studied porous wire material. After fixation of the bone tissue with the studied porous wire material and after decalcification of bone tissue of the studied porous wire material made finding and fixing the boundary wire element porous wire material in the bone block. Produced by Raspletina porous wire material from a bone block method of drawing the boundary wire element porous wire material. The analysis removed macroscopic specimen during the liberation parov�x spaces of the porous wire material. Extracted tissue released from the pore spaces of a porous wire material. Produce sampling and analyzing interstitial fragments porous wire material. Produced fill the bone tissue. Slices with a thickness of 5 μm is made on a rotary microtome Sakura with subsequent staining with hematoxylin and eosin and pikrofuksin by van gieson. Held histomorphometry of bone tissue. Evaluated the osseointegration of porous wire material in the experiment.

The positive effect of the proposed method is that it ensures the validity of the research results of osseointegration, analysis macropreparations with the release of the pore spaces of a porous wire material, allows the tissues released from the pore spaces of a porous wire material for histomorphometrical researches, creates the capability of sampling and analyzing interstitial fragments porous wire material.

The method can be applied and widely used in experimental medicine to study the effects of new composite materials on the process of osseointegration.

Method of evaluation of osseointegration of porous wire materials in the experiment, including the harvesting of bone blocks with the studied porous wire mate�Yalom; fixation of bone studied with porous wire material; decalcomanie bone tissue of the studied porous wire material; degreasing, dehydration of bone tissue of the studied porous wire material; filling of bone tissue; the production of sections of bone tissue; staining of bone tissue; bone histomorphometry, characterized in that before or after fixation of the bone tissue with the studied porous wire material and before or after decalcification of bone tissue of the studied porous wire material searches and fixation of boundary wire element porous wire material in the bone block; then produce Raspletina porous wire material from a bone block method of drawing the boundary wire element porous wire material; analyze macropreparations with the release of the pore spaces of a porous wire material; producing the tissues released from the pore spaces of a porous wire material; producing the sampling and analysis of interstitial fragments of porous wire material.



 

Same patents:

FIELD: medicine.

SUBSTANCE: general anaesthesia is performed. A skin incision 1.5-2 cm long is made with incising the skin, subcutaneous fat and fascia. The muscles attached to an infraspinous fossa of shoulder bone are skeletonised towards a spine; a caudal surface of a scapular spine is skeletonised; a blind bone bed is formed within the scapular spine at an angle of 45° to the surface of infraspinous fossa of shoulder bone. An implant of the analysed material is implanted into the bone bed to achieve primary stability. The wound is closed.

EFFECT: method provides minimum injuries accompanying the wound formation, reduces a risk of the incision wound damage by an animal, makes it possible to perform the surgery on opposite shoulder blade simultaneously, ensures the adequate volume and quality of the bone tissue in the surgical site, prevents the disordered muscular integrity throughout and loss of no more than 1 point of the muscular attachment.

2 dwg, 2 ex

FIELD: medicine.

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16 dwg

FIELD: medicine.

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8 cl, 3 dwg

FIELD: medicine.

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2 ex, 1 dwg

FIELD: medicine.

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2 ex, 6 dwg

FIELD: medicine.

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3 tbl

FIELD: medicine.

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FIELD: radio engineering, communication.

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3 cl, 4 dwg

FIELD: medicine.

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1 tbl

FIELD: medicine.

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1 tbl

FIELD: medicine.

SUBSTANCE: invention relates to medicine, in particular to traumatology and orthopaedics. Patient's body type is determined: brachymorphic (B), dolichomorphic (D), mesomorphic (M). X-ray examination of knee joint in frontal and lateral projections and patella in Merchant axial projection are performed. X-ray images are used to calculate patella length (Lp), width (Wp), thickness (Tp) by means of ruler or calliper. Model and size of endoprosthesis, anterior-posterior and medial-lateral dimension of its components, which must correspond to maximal extensibility (E) of wound, are determined by means of fitting templates with application of X-ray images. Angle of bending in knee joint (Ab), angle of patella rotation angle (Ap), value of maximal horizontal patella displacement in lateral direction (Pp) are specified. Obtained data are substituted into formula for calculation of sum of values of influencing factors Rm, Rb, Rd for each body type: for people of mesomorphic body type: Rm=(0.001385Ab+0.01032Ap+0.95102Pp-0.7712Tp+1.7949Wp-0.8902Lp+0.22803); for people of brachymorphic body type: Rb=(-0.00228Ab+0.0116Ap+0.072Pp-0.232Tp+0.2298Wp+1.1696Lp+0.4631); for people of dolichomorphic body type: Rd=(-0.001351Ab+0.01625Ap+0.968Pp-0.3574Tp+0.603Wp-0.1554Lp+2.809), where Ab is angle of bending in knee joint, degrees; Ap is angle of patella rotation, degrees; Pp is displacement of patella horizontally in lateral direction, cm; Tp is thickness of patella, cm; Wp is width of patella, cm; Lp is length of patella, cm; after which minimal value of skin cut is calculated: for people of mesomorphic body type P"з"m=(R-Rm)/0.1726; for people of brachymorphic type P"з"b=(R-Rb)/-0.1495; for people of dolichomorphic body type P"з"d=(R-Rd)/0.0533, where P"з"m, P"з"b, P"з"d is minimal value of skin cut for each body type, R is maximal extensibility of operation wound, Rm, Rb, Rd is sum of values of influencing factors for each body type, and mini-invasive endoprosthetics of knee joint with minimal traumatic cut is realised, if ratio of preliminarily determined parameters, satisfying given mathematical expression, is fulfilled for each body type.

EFFECT: method makes it possible to reduce complications due to determination and implementation of minimally traumatic and sufficient for operation performance access.

1 tbl, 3 ex

FIELD: medicine.

SUBSTANCE: operation is performed by skin puncture along the posterior surface of the shin bone in the proximal direction from the calcaneal tuberosity at 1-1.5 cm along the external edge of the Achilles tendon. A drill is inserted into the cavity of the subtalar joint, and the articular surface of the posterior calcaneoastragaloid articulation is treated. The articular surfaces of the anterior calcaneoastragaloid joint are treated from an additional approach by incising the skin not larger than by 0.5-0.8 cm along the dorsal external edge of the foot in a projection of the tarsal sinus. A cavity between the calcaneal and ankle bones is formed, wherein an autograft of the lateral femoral condyle is placed.

EFFECT: method eliminates deformities and pain syndrome by reducing surgical injuries and improving ankle vascularisation.

3 dwg

FIELD: medicine.

SUBSTANCE: method includes an external-lateral access, oblique osteotomy of the external ankle-bone above the joint fissure with the dissection of soft tissues in its front part, turning the ankle-bone outwards with the preservation of the connection with soft tissues, excision of cartilages of injured joints, displacement of the external ankle-bone with overlapping the arthrodised area to a tight contact with the tibia, talus and calcaneus with the formation of a united bone block and its fixation, lateral and longitudinal compression with the restoration of the extremity axis. Soft tissues are dissected in the distal tibiofibular joint of the front part of the ankle-bone through the external-lateral access. Osteotomy is carried out from outside inwards and upwards. The formed united bone block is fixed by a blocking pin intraosteally through the sole surface of the foot, with the restoration of the extremity axis under EIA control, with its introduction through the calcaneus, talus and lower third of the tibia. Then two fixing screws are introduced through the metatarsal tubercle in the sagittal plane. Axial compression, fixation by means of two blocking screws in the frontal plane in the middle-lower third of the tibia are realised. Lateral compression is realised by means of one screw through the talus and the other - through the proximal end of the external ankle-bone into the tibia in the frontal plane.

EFFECT: method eliminates the deformation of the foot and pain syndrome due to strong arthrodising of the ankle and subtalar joints and improvement of the talus vascularisation.

3 dwg

FIELD: medicine.

SUBSTANCE: interlaminectomy preserving a yellow ligament is performed. A sequestrum is visualised and removed. An EOC-controlled laser light guide is inserted into the intervertebral disk through its injured portion. That is followed by a laser vaporisation of a nucleus pulposus with a 3- or 4-pulse train, power 20 Wt, length 1 s, interval 2 s in a total dose of 940-1,000 J. An operative aid area is filled with Oxiplex® gel from epidural-periradicular-subligamentous direction.

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2 cl, 4 dwg, 1 ex

FIELD: medicine.

SUBSTANCE: cutaneo-subcutaneous flap is formed on a thumb stump by an arched incision, separated from the thumb stump with exposing a stump end face. A pedicle cutaneo-subcutaneous flap with its bed facing a back surface is cut out on the side surface of the third finger of the same hand at the level of the middle phalanx by an arched incision. The flap is mobilised; the formed defect is closed by a free skin graft. A bone or cartilage graft shaped after the nail phalanx is inserted into a deepening on the end face of the thumb. The cut out flaps are sutured together, and the formed nail phalanx is covered completely. The skin sutures are removed 2 weeks after the operation. The flap pedicles are dissected away on the third finger 4 weeks later.

EFFECT: method forms the nail phalanx by acceptance of the bone or cartilage graft to the nail phalanx stump.

6 dwg

FIELD: medicine.

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EFFECT: method enables reducing the episodes of sternomediastinitis, eliminating a residual cavity in the anterior mediastinum, providing higher strength of the thoracic wall frame, avoiding postoperative hernias, and improving the patient's quality of life.

1 dwg, 1 ex

FIELD: medicine.

SUBSTANCE: skin is incised 1 cm within an apex of ischial tuberosity. A closed reduction follows. After achieving a satisfactory contact of the fragments, under control of an electro-optical converter (EOC), a 3-mm screw of the required length is delivered along a descending branch of the ischial bone through a fracture line outside the hip joint cavity by means of a collet guide, into an iliac bone body to provide interfragment compression. Guide blades are opened, and the collet guide is removed from the wound. The second 3-mm screw is delivered through the same incision in the apex of the ischial tuberosity. The following stage involves delivering one 3-mm screw into a superior branch of pubic bone through the 1 cm skin incision from a point 2 cm above the cotyloid cavity, through the iliac bone body and fracture line.

EFFECT: method enables increasing the osteosynthesis stability.

4 dwg

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to traumatology and orthopaedics, and is intended for application in elimination of ankle dislocations. Additional ring support is fixed to base support, fixed to distal part of shin at level VII on rods in proximal direction. Distance between basic and remote support is determined from 5 to 8 cm. Diameter of remote support is selected to be 2-3 standard sizes larger than of the basic one. Distal support, which consists of two semi-rings, connected by bars, is fixed to foot bones in a single block. Additional support, fixed to basic support, and mobile support are connected by six strata. Fastening of strata to supports is realised in the following order: strata No 1, No 3 and No 5 are fixed to additional support at level IV of shin. Stratum No 1 is fixed in position 12, strata No 3 and No 5 in position 4 and 8, strata No 2, No 4 and No 6 are fixed to mobile support. Strata No 2 and No 6 are fixed to support at level of metatarsophalangeal joints, and stratum No 6 is fixed in position 6.

EFFECT: due to application of Ortho-SUV apparatus, working on the basis of computer navigation method makes it possible to set dislocations in ankle joint by apparatus in one stage for short period of time, with construction having minimal possible dimensions during fixation period.

46 dwg, 1 tbl, 1 ex

FIELD: medicine.

SUBSTANCE: one of ends of a periosteal-cortical graft is separated from the periosteum by stripping and turning it off together with feed vessels. A niche is formed in an end face portion of a projecting bone fragment at the level of the bed depth formed in an opposite bone fragment, and at the height enabling bringing one of the ends of the bone graft behind. The periosteum-free end of the bone graft is brought into the niche in the projecting bone fragment, pressed to the bed surface in the opposite bone fragment and fixed with elastic tension. The stripped periosteum is laid on the end face of the projecting end fragment and fixed.

EFFECT: method enables improving functional results, reducing the length of treatment.

FIELD: medicine.

SUBSTANCE: bone integrity is broken by osteotomy. The method involves close skeletisation of an endoprosthesis leg end, and an intramedullary rod is inserted into the thigh canal through a knee joint by means of a conductor guide up to a place of osteotomy. That is followed by percutaneous osteotomy of the thigh, and the intramedullary rod is further advanced until it connects to the endoprosthesis leg. In a front plane, 3-4 rods are delivered into a distal thigh and fixed on an outer support, which is connected by a compression-distraction rods to a support mounted on the conductor guide of the intramedullary rod. The compression-distraction rods are used to elongate the rod to the required size. The intramedullary rod is blocked with 2-4 thigh stabilisation screws. The supports and conductor guide are demounted, the pins are removed.

EFFECT: method enables reducing injuries and providing the previously recovery of support ability.

5 dwg

FIELD: medicine.

SUBSTANCE: bioptic skin tissue with particles of implanted tattoo pigment is sampled. The samples are used to determine the tattoo pigment depth. The most effective laser wavelength is determined by exposing the tissue samples to laser light at various wave lengths. The samples are coloured, and those suffered the most severe damage of the tattoo pigment are detected. If the measured tattoo pigment depth is no more than 0.7 mm, the laser removal of the tattoo pigment is initiated. If the measured tattoo pigment depth falls within the range of 0.7 mm to 2.0 mm, the superficial destruction is expected to be followed by the laser removal of the tattoo pigment. The most effective laser wavelength determined by the bioptic tissue sampled is specified for performing the removal procedure.

EFFECT: method enables providing more effective tattoo removal by eliminating the necessity of the excessive thermal exposure of the patient's skin, reduces a probability of the involvement of the adjoining biological tissues, and eliminates the colour inversion.

3 ex

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