Method of human immunomodulation
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to pharmaceutical industry, namely to means, possessive immunomodulating action. Method of human immunomodulation by application of live suspension of chlorella of strain Chlorella vulgaris IGF No C-111 with concentration 8-10 millions cell/ml in quantity 200 ml per day for adult human.
EFFECT: method makes it possible to increase human immunity effectively.
The invention relates to pharmaceutical industry, and more specifically to means of having immunomoduliruushim effect.
Currently developed and found wide practical application, a large number of funds, including dietary supplements (the SUPPLEMENTS) to food containing different natural bioactive compounds.
Especially important in this spectrum is a matter on the basis of microalgae and, in particular, Chlorella. Thanks to its unique natural composition and high concentration of biologically active substances, it differs from higher plants (1).
Chlorella is an active producer of biomass with a large percentage of complete protein, fats, carbohydrates, vitamins ... just over 650 substances in concentrations that far exceed their content in traditional foods: meat, milk, vegetables and fruits.
Chlorella belongs to the category of "Superfoods" such as spirulina, green barley and wheat, pollen (bee products), propolis and Royal jelly, which gathered every one of the nutrients required for the germ of future life and ensuring a prosperous existence of all species (2).
Today there is a wide range of substances on the basis as soon as Chlorella, or in combination with other Mick�vodoroslami and components.
However, in all known substances and food additives on the basis of Chlorella, use only dry microalgae biomass or extracts and extracts of dry Chlorella. This is due primarily to the properties themselves strains of Chlorella used to obtain such substances. It is known that in the world for the production of dietary SUPPLEMENTS used the so-called soil strains of Chlorella, which has a low antibacterial properties and is not able to be kept in suspension. After cultivation of a suspension of Chlorella soil strains, cells of Chlorella are separated from the culture medium, then the resulting paste is dried, yielding a dry microalgae biomass, which is used for the production of dietary SUPPLEMENTS. In such cases, the culture medium of Chlorella is not used.
It is scientifically proven that a number of important and valuable biologically active substances produced in plants is able to maintain their properties only in a living cell. Conversely, after heat treatment plants, such substances are subjected to decay and lose their active properties. In addition, after drying, plant cells, many biologically active substances are not able to be stored for any extended period of time, subjected to oxidation, decomposition and during storage gradually lose their properties.
Another method of preparation of medicinal p�of aparatow from medicinal plants and Chlorella based on the preparation of alcoholic liquors or extracts. This provides without any heating good enough sterility drugs, however, plants may not be removed some medicinal active substances (3).
The best way out of this situation is the ingestion of natural food like herbs, vegetables, fruits, etc., which has medicinal properties. However, this possibility is usually missing in most of the year, which requires the processing and harvesting of plants for future use. Can plants to dry, crush, cook consumer shape in the form of finely divided powders, tablets and capsules (with the addition if necessary of minerals). However, in these cases, without additional measures for the sterilization of such dosage forms from natural raw materials, their ingestion is often unacceptable because of the potential for microbial contamination of raw materials (3).
It is known (4) that in the process of photosynthesis of Chlorella stores in its cells and secretes into culture medium a wide range of substances with pronounced biological activity, and biochemical composition of the biomass of Chlorella cells (355 substances) and the culture medium of Chlorella (312 substances) contains about the same amount and composition of biologically active substances.
Modifying the conditions of cultivation of Chlorella and composition of the nutrient medium, m�can purposefully change the content of the main components in biomass of Chlorella (4).
Numerous experiments with Chlorella on animals in this country and abroad, clearly proves the superiority and the effectiveness of the use of a suspension of Chlorella in comparison with dry Chlorella or pasta (5, 6).
Obviously, from the point of view of better conservation of the entire complex of biologically active substances synthesized by the Chlorella, it is necessary to use as the living cell microalgae and all exudates secreted by Chlorella in culture medium in the process of photosynthesis. Only in this case not only the best possible preservation of all biologically active substances, but also ensuring the maximum effect of its application.
The main reason that soil strains of Chlorella could not be used as a suspension, is the absence of a strong bactericidal properties, which would be able to suppress or stop the development of concomitant microflora in the process of their storage. For this reason, the cells of the soil strains of Chlorella after cultivation immediately separated from the culture medium and dried. The dry powder is then as a rule, tableted, and is used, for example as a nutritional Supplement.
Such powders on the basis of dry cells of Chlorella have a number of disadvantages.
1. During the processing and drying of cells of Chlorella PR�come the loss of some important biologically active substances, can survive only in living cells of Chlorella.
2. The digestibility of the remaining biologically active substances in the dry cell of Chlorella significantly lower than in a living cell.
3. Do not use a wide range of biologically active substances, which it secretes into the culture medium in the process of photosynthesis.
4. Long shelf-life food additives on the basis of dry cells of Chlorella inevitably lead to the impoverishment and deterioration of the composition of biologically active substances due to their gradual oxidation and decay.
Thus, the prepared additives on the basis of dry Chlorella potentially on its biological values are an order of magnitude below the slurry of Chlorella, from which they are derived.
The object of the present invention is to provide means having immunomoduliruushim action on the basis of living cells microalgae Chlorella strain of Chlorella vulgaris and exudates secreted it into the culture medium, namely, live suspensions of Chlorella.
To solve this problem is proposed to use planktonic strain of Chlorella vulgaris IFR number C-111 (SU 1751981, C12N 1/12, 10.02.1997) or obtained on its basis the child strains of Chlorella with high antibacterial qualities. The strain of Chlorella vulgaris IFR number C-111 is the parent strain for strain BIN (RU 2192459, C12N 1/12, C02F 3/34 18.04.2001) and strain Parachlorella nurekis 1904 KIEG (301670, C12N 1/2, 19.05.2010). Both are subsidiaries of the strain are planktonic with high bactericidal properties. Chlorella vulgaris BIN that is designed to produce biomass and wastewater treatment, Parachlorella nurekis 1904 KIEG used to destroy the blue-green algae, bacteria and fungi when legalizazii reservoirs. They both can be used as producers of biomass, and therefore as BAD in the inventive form of a suspension, as they have the same high bactericidal properties as their parent strain IGF No. P-111.
Properties of planktonic strains of Chlorella are fundamentally different from all previously known soil strains:
1. The ability to be stored only in the form of a suspension in a conventional, non-sterile conditions due to their high antibacterial qualities.
Soil strains can only be stored on solid nutrient media on agar-agar, in the special conditions and in compliance with the requirements of sterility in their passage.
2. To be in a planktonic state or condition of floating in a culture medium in the culturing process, and at rest or storage, due to the presence of weakly negative charge of cells of microalgae.
Cells soil strains have no electric charge, quickly settle to the bottom and require constant agitation during the whole time of cultivation.
3. Cultivation PLA�kennyh strains produced in monoculture, the presence of other species of algae and even other strains of Chlorella is not allowed.
For the cultivation of the soil strains of Chlorella in the culture, there are always other types of algae and strains of Chlorella. In practice, often done simultaneously cultivating several strains of Chlorella.
4. Planktonic strains differ by an order of magnitude more efficient, for example in animal husbandry norms of feeding suspensions has decreased ten times in comparison with soil strains at the same density of cells in suspension and the same results.
5. Planktonic strains were significantly greater extent neprihotliva soil, in their cultivation, both in terms of conditions and equipment design, and culture media.
With the right cultivation of planktonic strains of Chlorella and maintaining a strong culture, thanks to a pronounced antibacterial properties, it is possible to stabilize the performance of QMAFAnM the requirements of SanPiN 126.96.36.1998-01, requirements for BAA on the basis of microalgae, in the absence of indicators on BGK (coliforms), pathogenic microorganisms, including Salmonella, yeast and mold.
Method of cultivation of planktonic strains of Chlorella provides for the filling of the nutrient medium in the container, the inoculation suspense�and strain, lighting of the culture vessel during the growth of microalgae and maintaining the necessary temperature of the suspension. The cultivation of microalgae is produced in special plants cultivators of Chlorella. They represent the capacity of a transparent material (glass, plexiglass) in a metal frame with outer and inner sources of artificial light, and automatically maintain the desired temperature in the suspension.
After the cultivation and growth of a given cell density of Chlorella bacterial counts in the suspension remains normal for a long period of storage under normal conditions without the need for its preservation or freezing. Such high bactericidal properties are achieved because of the commitment of cells of Chlorella antibiotic substances in the culture medium. In this case the antibiotic action of these substances is pronounced only in respect of a wide range of pathogenic microorganisms and does not affect the intestinal microflora.
Example 1. A method of producing BAD
For the cultivation of microalgae from the strain of Chlorella vulgadis N With IGF-111 prepared nutrient medium:
Ammonium nitrate - 0.2-0.4 g
Superphosphate, 10% solution - 0.3-0.5 ml
Chloride of iron, 1% solution, 0.15 ml
Nitrate cobalt, about 0.1% �Astor - 0.1 ml
Copper sulfate, 0.1% solution 0.1 ml
Carbon dioxide, the solution (pH 3.0 and 5.0) - 5-10 ml
Tap water - 1000 ml.
The beginning of the cultivation of Chlorella. The nutrient medium at a temperature of about 30°C is poured into the apparatus for the cultivation of Chlorella in the amount of 80% of the volume of the container and injecting a suspension of strain of Chlorella vulgaris IFR With N-111 in the amount of 20% of the volume capacity of the installation to achieve the desired initial cell density (2 million/l) in culture. Then make a solution of carbon dioxide in an amount of 0.5 to 1.0% by volume of a suspension of Chlorella and include sources of lighting setup. In the further cultivation of Chlorella solution of carbon dioxide is injected every day in the form of Buxus getting suspension (pH 3.0 to 5.0) once a day in an amount of 0.5-1.0% in the moment of switching on of the lamps of the installation. Cultivation of Chlorella in the installation lasts four days to achieve the desired density of cells in culture in an amount of 8-10 million/ml. the duration of the illumination lamps of culture is 12-14 hours a day, rest of the day the lamp is off. The temperature in suspension in the culturing process is maintained at 30°C. After four days of cultivation, the cell density in the suspension reaches 8-10 million/ml. Suspension of Chlorella merges with the installation and as such is ready for use as a nutritional Supplement.
In the same way carbon dioxide and dust.�sly gas can be fed in the form of a mixture with air in the amount of 1-2% by volume of air through a compressor and spray gun located on the bottom of the tank installation. The feed rate is 10-20 liters per 1 m3/min.
Willing suspension of Chlorella may also continue to segestica to produce concentrated Chlorella (PFS) with a cell density of 20 to 1000 million/ml.
Example 2. A method for concentrating a suspension of Chlorella.
According to a known method (SU # 1091890) initial suspension of Chlorella subjected to electro-flotation resulting from the electrolysis of water the bubbles of oxygen, charged positively contribute to the ascent and the concentration of biomass at the surface, then the biomass is removed in the receiver.
We have found that when added to a highly concentrated biomass of Chlorella culture medium or suspension of Chlorella in a certain proportion, sufficient to ensure cell densities from 30 to 1000 million/ml in the resulting concentrate biomass of Chlorella, you can get the product is a concentrated biomass of Chlorella cells (KBH), which already can be stored for at least 30 days under normal conditions.
Practically this is achieved by removing foam from the surface of the suspension with electro-flotation occurs simultaneously with a small amount of a suspension of Chlorella, i.e. the foam is taken not in its pure form, but with the suspension of Chlorella. Suspension of Chlorella has high antibacterial properties�AMI and acts as a preservative even at higher cell densities 100 times than in the initial suspension of Chlorella.
The resulting purified the culture medium of Chlorella (COAG) is also a valuable biological product, which in solution is a wide complex of biologically active substances - exudates cells of Chlorella (Chlorella, physiologically active substances and their use. S. S. Melnikov, E. E. Anankina. Institute of photobiology of the an BSSR, Minsk, 1991 ).
To thicken the purified culture medium of Chlorella (COAG) to values from 5 to 100 times using the known method of reverse osmosis or ultrafiltration.
By way of reverse osmosis is the concentration of all substances in the purified culture medium (exudates cells of Chlorella) and a selection of pure solvent (water) from the solution, except for a certain amount of monovalent ions Na, K, Cl. Almost the reverse osmosis is reduced to the concentration of the purified culture medium of Chlorella and obtaining the product of concentrated culture medium of Chlorella (CCSH). This method of thickening to the culture medium of Chlorella nobody has used. Reverse osmosis is today a great variety. Concentration is carried out at least on the order, and if necessary, 30-50 times. Shelf life CCSH is not less than 3 months.
The concentration�probing can be performed by ultrafiltration. By pumping the culture medium is filtered under pressure inside-out hollow fiber UF module. The purified water passes through the porous walls of the hollow fibers and then used as a normal clean water. The particles of the culture medium of Chlorella size of 0.01-0.1 μm remain inside hollow fiber UF module. As a result of leaching of the fibers in the drive gelled culture medium is collected, concentrated culture medium of Chlorella.
The ultrafiltration method is simpler in its execution, but allows you to concentrate just a certain range of substances the culture medium of Chlorella, the size of the molecules which are in the range from 0.01 to 0.1 μm, which is its major drawback.
To obtain KH mix CCCH and KBH. The number KBH and CCSH depends on the concentration (the degree of thickening relative to the source) CBH and CCSH. Can be mixed in various concentrations. If we bear in mind the maximum range of ratios CBH and CCCH in KH, in our case, the AB will be a range of products, from one side, with a maximum cell concentration of Chlorella and minimum concentration CCCH (i.e. CBH), with different minimum cell concentration of Chlorella and a maximum concentration of CCCH (i.e. CCCH). For BAD obtained the following range: density cle�OK from 1 million/ml, to 1000 million/ml (leave minimum) and liquid medium is CXH by a factor of thickening from 2 to 100 times.
In some cases, obtaining CH advisable from a single volume of the initial suspension, i.e. receive and thickened biomass CBH, and thickened CCCH and then mix them. In this case we can speak about the product, thickened relative to the source of the commodity suspension several times (10, 15, 20, 25, 30, etc.).
Suspension of Chlorella with a density of 8-10 million cells/ml or concentrated Chlorella (PFS) with a cell density of 20 to 1000 million/ml can be stored under normal conditions at a temperature of from 0 to 24°C not less than 45 days from the date of receipt. In the process of storage cells of Chlorella after a few days gradually begin to settle to the bottom. Not having conditions for photosynthesis, the cells of Chlorella gradually fall into a state of suspended animation, with all the settling cells to the bottom occurs in approximately one month. However, the cells of Chlorella remain alive and are ready at any time to photosynthesize. So before use a suspension of Chlorella to stir.
In order to increase the shelf life of suspensions of concentrated Chlorella and Chlorella (CH) to 6 months is proposed to use carbon dioxide. Mass fraction of carbon dioxide to a suspension of Chlorella is, for weakly carbonated on�ickow (in accordance with GOST 28188) 0,20-0,30%, inclusive. In this form the cells of Chlorella receive the necessary amount of carbon dioxide for the implementation of your breathing during storage in a tightly closed state.
Our studies on the test volunteers showed that the proposed live suspension of Chlorella strain of Chlorella vulgaris IFR number C-111 concentration of 8-10 million cells/ml in an amount of 200 ml per day was highly effective drug.
Male, age 43 years, took a suspension of Chlorella 200 ml per day for one month. Data of immunograms given before and after the adoption of a suspension of Chlorella:
Male, age 44 years, brought a suspension of Chlorella 200 ml per day for one month. Data of immunograms given before and after the adoption of live suspensions of Chlorella strain of Chlorella vulgaris IFR number C-111
The tests of examples 3 and 4 was conducted on the basis of the Penza regional center for AIDS prevention, where studies have been conducted to study the immunostimulatory properties of the microalgae Chlorella vulgaris (strain IGF no. C-111) on immunological, biochemical and clinical parameters people. The research has established a stimulating effect on phagocytosis, humoral immunity and kletocnah� immunity in both patients. Both volunteers noted a steady improvement in their health since the early days of the use of the drug. Among the examined volunteers recorded a significant improvement of the parameters of immunogram during the observed month relative to the beginning of the experiment. In biochemical studies, both patients had normalization of most of the indicators.
Thus, the results of the research showed immunostimulatory effect of suspension of microalgae Chlorella vulgaris strain IGF no. C-111 on the immune cells both participated in the experiment volunteers.
A group of volunteers from eight people: three men and five women. Age men from 43 to 45 years of age, two women, 30 and 40 years and three women over the age of 65. One of the women at the age of 72 was moved to experiment two stroke. The experiment was conducted at the Department of immunology and Allergology of the Central research laboratory of Penza Institute of advanced medical.
Conclusion: On the basis of the Central research laboratory of Penza postgraduate medical Institute in the period from 21 March to 15 may 2006 studies have been conducted to study the immunostimulatory properties of the microalgae Chlorella vulgaris (strain IGF no. C-111) on immunological, biochemical and clinical parameters people. The subjects are volunteers in the amount of 8 people for m�the month took the studied microalgae 100 grams 2 times a day. Studied parameters were taken before the experiment and after the completion of the reception of this microalgae.
The research has established a stimulating effect on phagocytosis in 3 patients, the humoral immunity in 4 patients, cell-mediated immunity in 8 patients. In biochemical studies, all patients had normalization of most of the indicators. Clinical blood analysis did not reveal any changes in the blood picture.
Thus, the results of the research showed immunostimulatory effect of live suspensions of Chlorella strain of Chlorella vulgaris IFR number C-111 on immunocompetent cells of all participants in the experiment people.
The Department SHOWRUN in the period from 24 March to 15 may 2008, studies have been conducted to study the effects on the human body food suspensions of live microalgae Chlorella vulgaris (Strain IGF no. C-111). We analyzed immune-boosting properties and impact on immunological, biochemical and clinical parameters people. The subjects are volunteers in the amount of 20 people (1) and 10 primates (2) within 15 days study took food microalgae suspension of 100 grams 2 times a day. Studied parameters were taken before the experiment and after receiving this �trovadores. In the list of studies included: immunological studies of blood - total immunity, cellular immunity, the rate of total IgE, obsidianstone biochemical analysis. Additionally the group (1) were analyzed: Bioelectrography (assessment of autonomic functions), EHP assessment of the degree of contamination of the organism on the basis of a specialized iridodiagnosis research "iris-Sigma", evaluation of visual function and neurophysiological indicators - method color campimetry, these electrophysiological studies, subjective feelings and objective data of inspections.
The research was conducted jointly with the City Centre for combating AIDS and infectious diseases, ocular area with the endoecological rehab sanatorium "October", research Institute of medical Primatology RAMS, Sochi.
The research has established a stimulating effect on phagocytosis in 65% of patients, the system humoral immunity in 70% of patients cellular immunity in 84% of the examined patients. In biochemical studies, 80% of patients had normalization of most of the indicators. Marked improvement in autonomic functions and the decrease in the percentage of tissue, lymphatic and subcutaneous fat of pollution, improvement of visual functions, neuroscientists�die and electrophysiological indicators of 15-25%. Clinical blood analysis revealed an improvement in the blood picture, according to the following criteria: the color index, thrombocyte, lymphocytes, segmented, eosinophils, ESR in 68% of cases.
Thus, the results of the research showed immunostimulatory effect of live microalgae Chlorella vulgaris on the immune cells involved in the experiment people.
Male, age 78 years. Every day receives a suspension of Chlorella in an amount of not less than 1.5 liters per day for the past 5 years, completely replacing her water in your diet. Since the beginning of the use of a suspension of Chlorella felt a significant improvement in health. Gradually got rid of a number of diseases, including chronic nature of the digestive system, periodontal disease and colds. During the use of a suspension of Chlorella never went to doctors. Assess their condition, as a healthy 50 year old male.
According to our observations the use of a suspension of Chlorella in an amount of 200 ml per day for all volunteers in the first week leads to sustained improvement in General well-being. Improvement of digestion and normalization of stool, a surge of strength and health, shortened sleep and faster recovery after exercise. Chlorella improves brain�qau circulation and memory starting to clear head to work, normalizes blood pressure, strengthens the immune system. During the intake of Chlorella volunteers experienced significantly reduced symptoms of pre-existing diseases.
Experience of the use of substances on the basis of Chlorella in the world proves wide range of effects of this Alga on the human body. Unsurpassed effect identified with the use of live Chlorella strain of Chlorella vulgaris IFR number C-111 for the removal of various endogenous and exogenous intoxication, alcoholic hangover, in the treatment of constipation, stomach ulcers, allergies, arthritis, atherosclerosis, elevated cholesterol levels, hypertension, cardiovascular disease, and also as antiviral agents, etc. the Overall positive impact of the suspension of Chlorella on the human body can be described as a significant and sustainable improvement in the quality of life.
Visible and sustainable improvement of the General condition observed during a certain continuous infusion of dry Chlorella. It is obvious that the substances on the basis of dry cells of Chlorella certainly have a positive effect on the human body, as after cell separation, drying or extraction of the composition of biologically active substances in them is still quite high. However, in the use of live suspension x�orally strain of Chlorella vulgaris IFR number C-111 man such sustainable improvement comes almost from the first week of admission. This positive result is achieved mainly at the expense of living forms the substance - of a suspension of Chlorella strain of Chlorella vulgaris IFR no. C-111, allowing not only to maintain a full range of all the biologically active substances in the cell microalgae in a culture medium, but also conducive to maximum absorption of these substances by the human body.
Thus, for the first time to use a man offers a living plant in the form of suspension of microalgae on the basis of planktonic strains of Chlorella strain of Chlorella vulgaris IFR no. C-111, a unique antibacterial properties which help to keep the cells in suspension in this form for a long time under normal conditions without the need for preservatives or freezing.
Suspension of Chlorella strain of Chlorella vulgaris IFR number C-111 can be used in the form of live suspensions with a density of cells obtained immediately after cultivation in an amount of 8-10 million/ml, and in thickened form, with a cell concentration of 20 to 1000 million/ml.
The list of references
1. Mike Adams. Superfoods For Optimum Health: Chlorella and Spirulina.
2. Robert Atkins. Supplements: a natural alternative to drugs.
3. Patent RU (11) 2178660 (13) C2.
4. S. S. Melnikov, E. E. Anankina. Chlorella: physiologically active substances and their use. Minsk. �Science and technology", 1991
5. J. M. Salnikova, Chlorella is a new kind of feed, Moscow, KOLOS, 1977
6. Muzafarov A. M., Taubaev T. T., Salamatov R. A., Chlorella vulgaris and its use in livestock, Tash., 1974.
A method of immunomodulation of human rights by taking in a live suspension of Chlorella strain of Chlorella vulgaris IFR number C-111 concentration of 8-10 million cells/ml in an amount of 200 ml per day.
FIELD: medicine, pharmaceutics.
SUBSTANCE: group of inventions refers to medicine and concerns an adjuvant immunogenic composition containing meningococcal lipooligosaccharide (LOS) and serotype 14 pneumococcal capsular saccharide (CS14), wherein CS14 contains tetrasaccharide Galβ1-4GlcNAcβ1-3Galβ1-4Glc, while LOS is free from tetrasaccharide Galβ1-4GlcNAcβ1-3Galβ1-4Glc. The group of inventions also concerns a method for inducing the immune response in a mammal involving administering the above composition.
EFFECT: group of inventions provides the stronger immune response to CS14 A as compared to wild-type OMV containing LNnT.
15 cl, 4 dwg, 1 ex
SUBSTANCE: invention refers to agriculture, particularly to veterinary medicine, and aims at providing the more effective mosquito puncture procedures for the purpose of treating subclinical mastitis in cows by the effect of mosquitoes on biologically active points. That is ensured by the fact that the exposure covers the biologically active points from both sides of a sacral bone by introducing 20 mosquitoes for 4-5 minutes. The procedures are performed four times every 24 hours.
EFFECT: method provides the clinical effectiveness of 93,3% and is less labour-consuming and environmentally safe as compared to the other methods.
SUBSTANCE: invention refers to agriculture, particularly to veterinary medicine, and aims at providing higher clinical effectiveness in latent mastitis in cows. That is ensured by using mosquito's salivary secretions as biologically active substances. To this effect, 20 laboratory mosquitoes are introduced on the skin of the affected udder lobes. The treatment is administered for 3 days every 24h at any time of day.
EFFECT: method provides the 100% clinical effectiveness in latent mastitis, being less labour-consuming and environmentally safe as compared to the other methods.
SUBSTANCE: group of inventions refers to medicine, and concerns an immunostimulant composition containing a compound of formula I; to a method for enhancing the immune response to an antigen in an individual, involving administering the antigen and composition into the individual. Enhancing the immune response can represent enhancing the antibody-mediated immune response, CD4+ T-cell response, CD8+ T-cell response or activating the antigen-presenting cells. What is also presented is a method for enhancing the immune response by administering the antigen and composition containing the compound of formula I intramuscularly.
EFFECT: group of inventions enables achieving enhancing the immune response to the antigen.
24 cl, 7 ex, 13 dwg
FIELD: medicine, pharmaceutics.
SUBSTANCE: group of inventions refers to medicine and concerns a pneumococcal vaccine containing saccharide of 4, 6B, 9V, 14, 18C, 19F and 23F serotypes, separately conjugated with CRM197, and at least one Toll-like receptor-9 (TLR-9) agonist as an adjuvant, wherein the above Toll-like receptor-9 agonist represents CpG-oligonucleotide. Also, the group of inventions concerns using the pneumococcal vaccine for preparing the therapeutic agent for preventing or treating diseases caused by S.pneumoniae; a method for individual's immunisation against the diseases caused by S.pneumoniae infection, involving administering the immunoprotective dose of the vaccine into the above individual.
EFFECT: adding B-class CpG to the conjugated pneumococcal vaccine provides the considerable increase of the proportion of highly responsive patients, good tolerance and cell memory induction in relation to pneumococcal polysaccharides.
45 cl, 9 dwg, 4 tbl, 3 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: claimed is a group if inventions: a pharmaceutical composition, containing immunologically active enantiomer of a component of the cationic lipid R-DOTAP or S-DOTAP, a method of applying the said enantiomer for induction of an immunostimulating adjuvant effect in the immune system, a method of inducing immune response in a patient with applying thereof and a method of treatment or prevention of a disease in the patient by induction of the immunostimulating adjuvant effect by the lipid.
EFFECT: R-DOTAP and S-DOTAP induced production of cytokines IL-2, IL-8, IL-12, chemokines CCL-5, CCL-19 and tumour regression in mice.
23 cl, 15 dwg, 8 ex
SUBSTANCE: abdominal paracentesis is performed to remove ascetic fluid to be concentrated and filtered by means of hemodialysis filter and plasma filter. The prepared concentrate is added with human recombinant interleukin-2 in a dose of 500 IU. The concentrate is refused intravenously at a perfusion rate of 60 drops a minute in an amount of 500 ml. On the day following the abdominal paracentesis, the concentrate is introduced again.
EFFECT: method provides effective elimination of hypo- and dysproteinemia in the given category of patients, as well as the correction of immune disorders by using plasma self-proteins and cytokines that enables the further surgical correction of the detected pathology.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to medicine, namely to immunology, and can be used for obtaining medication for preventive treatment of tuberculosis. Immunotherapeutic medication contains fragments of cell wall of Mycobacterium tuberculosis complex (MTB-C).
EFFECT: claimed medication is obtained by method including the following stages: cultivation of virulent strain of MTB-C for the period, equal or longer than three weeks, and homogenisation of cell culture in presence of non-ionic surface-active substance.
14 cl, 2 ex, 2 tbl
FIELD: food industry.
SUBSTANCE: inventions group relates to food industry. The nutritive composition for improvement of the immune system with a mammal, preferably, with the human includes the following components: (a) at least 18 en% of protein material; (b) at least 12 wt % of leucine of the total quantity of protein material; (c) lipid fraction including at least one ω-3 polyunsaturated fatty acid chosen from the group consisting of eicosapentaenoic acid, docosahexaenoic acid, eicosatetraenoic acid and docosapentaenoic acid; (d) immunomodulator.
EFFECT: inventions group ensures intensification of protective immune response to an exogenous pathogene or autologous trigger such as neoplastic cells as well as improved humoral immunity or their combination.
17 cl, 4 dwg, 6 tbl, 6 ex
SUBSTANCE: invention refers to veterinary science, concerns a method for correction of secondary immunodeficiency in calves. A phytopreparation representing a 70% alcoholate of purple Echinacea (Echinacea purpurea L.) herb and blossom clusters, common pine (PHnus sylvestris) buds, yellow starwort (Inula helenium) roots and rhizomes, common licorice (Glycyrrhiza glabra L.) roots, common harmala (Peganum harmala) herb taken in the ratio 1:1:1:1:0.5 is administered orally in clinically healthy calves in the form of 7-8% aqueous solution in a dose of 2.0-2.5 ml/kg of an animal's body weight for 10-15 days every 24 hours.
EFFECT: invention provides correcting secondary immunodeficiency to physiological standard that enables preventing gastrointestinal and respiratory diseases in calves.
4 tbl, 4 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to the pharmaceutical industry, namely to a method of obtaining pectins from a biomass of cultured tissues of plants Silene vulgaris (M.) G. The method of obtaining pectins with an increased content of galactose residues in side carbon chains from Calusa cultures includes raw material destruction, extraction with water, processing of the biomass with hydrochloric acid, water washing, extraction with ammonium oxalate solution, sedimentation of polysaccharide with ethanol, dialysis and lyophilising, with Caluse cultures being preliminarily grown for 21 hours on an agarised nutrient medium, which contains the ferment 1,4-β-D-galactozyltranspherase in a specified concentration, and as the raw material the biomass of cultured tissues of plants Silene vulgaris is applied.
EFFECT: method makes it possible to obtain physiologically active pectins, which have a specified structure and stable chemical composition.
1 tbl, 4 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: group of inventions refers to an oral care composition, methods for preparing and using it. The presented composition containing a pre-mix containing arginine in the free form or in the form of a salt, a soluble carbonate salt and arginine bicarbonate formed in situ when mixing arginine in the free form or in the form of a salt with the soluble carbonate salt. The method for preparing the composition involves preparing the pre-mix by mixing arginine in the free form or in the form of the salt and soluble carbonate salt. What is also presented is an oral care procedure involving coating the patient's oral cavity with an effective amount of the above composition.
EFFECT: using the group of inventions provides positive effects achieved by using the oral care composition containing arginine bicarbonate in a combination with a simplified process of preparation of this composition.
15 cl, 2 ex
SUBSTANCE: invention relates to method of obtaining rosemary microcapsules. Claimed method is characterised by the fact that mixture of rosemary and dimethylsulphoxide is dispersed in suspension of carrageenan in ethanol in presence of preparation E472c, with the following addition of toluene and water, obtained suspension of microcapsules is filtered and dried, with ratio core/envelope in microcapsules constituting 1:1 or 1:3.
EFFECT: method by invention ensures simplification and acceleration of process of obtaining microcapsules, as well as increase of output by weight.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to the pharmaceutical industry, namely to a formulation of a cough medical composition. The formulation of the cough medical composition contains an active substance presented by thermopsis herb powder or a dry extract of thermopsis and sodium hydrocarbonate, as well as an excipient, a granulating agent and a lubricant taken in certain relations (versions).
EFFECT: composition of the cough medical composition possesses improved pharmaceutical (appearance, taste) and technological characteristics (hardness, disintegration).
11 cl, 7 ex
SUBSTANCE: method for photodynamic inactivation and inhibition of growth of Enterococcus faecalis bacteria, based on pretreatment of the bacteria with a hypericin-containing photosensitiser based on an alcoholic tincture of hypericum "Diahyperon" or "Hypericum tincture" and subsequent exposure of the obtained suspension to optical radiation of laser or LED sources with transmission maxima in the region corresponding to absorption spectrum maxima or maxima of the excitation spectrum of fluorescent components of the preparations with radiation energy density of 0.9-9.0 J/cm2, under certain conditions.
EFFECT: method simplifies inactivation of Enterococcus faecalis bacteria.
22 cl, 7 dwg, 3 tbl
SUBSTANCE: stellaria grass infusion is introduced to laboratory animals in a dose of 5 ml/kg of weight 20 minutes before irradiation in an ultraviolet chamber for 14 days daily.
EFFECT: stable pharmacological effect under conditions of reduction of the phytocorrection course duration.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to the field of pharmaceutics and represents a composition for oral cavity care in the form of dental varnish, containing bioactive glass and fluoride, where the said fluoride is present in the composition in an amount of up to 5% by weight. An increased ionic release is observed for the dental varnish containing bioactive glass and fluoride.
EFFECT: invention provides the creation of compositions, containing bioactive glass, for which in case of applying fluoride in lower concentrations (lower than 900 mln-1) its efficiency is the same as for higher concentrations (900 - 1450 mln-1).
3 cl, 2 ex, 6 tbl
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to pharmaceutical industry, in particular to application of composition for preparation of medications aimed at secondary prevention of cardiac infarction. Application of composition based on substances, applied in Chinese medicine, for preparation of medications aimed at secondary prevention of cardiac infarction, with composition, based on substances, applied in Chinese medicine, is prepared from composition, which contains Radix Astragali, Radix Salviae Miltiorrhizae, Radix Notoginseng and Lignum Dalbergiae Odoriferae, taken in specified ratio.
EFFECT: composition makes it possible to prepare medication, which is effective for secondary prevention of cardiac infarction, prevents stenocardia, improves coronary blood flow.
14 cl, 8 dwg, 74 tbl, 10 ex
SUBSTANCE: invention relates to a compound with a woody note having structural formula I. In formula , the ring with 6 carbon atoms is saturated or has a double bond between carbon atoms C1 and C2 or between carbon atoms C1 and C6, R is selected from a C2-C5 alkyl or C2-C5 alkenyl group.
EFFECT: invention also relates to a fragrant composition containing said compound.
13 cl, 16 ex
SUBSTANCE: method includes carrying out complex treatment at the background of diet therapy. Intake of antihelmintic of vegetable origin and immunomodulator is carried out daily with washing down each of them with 200 ml of radon water. Intestinal lavage is performed every second day. On days of performing intestinal lavage, patient takes bath with radon water in the morning before lavage, with performing underwater hydrodynamic massage (UHM) on other days. On days, when intestinal lavage is not performed, sessions of sound therapy are carried out after UHM.
EFFECT: method provides correction of biological age of organism as prevention of premature ageing.
3 cl, 4 tbl, 2 ex
FIELD: medicine, oncology, amino acids.
SUBSTANCE: invention relates, in particular, to the development of an antitumor preparation based on natural substances. Invention relates to an amino acid preparation comprising at least one modified essential amino acid obtained by treatment of amino acid by ultraviolet radiation (UV) at wavelength 250-350 nm for 12-80 h at temperature 15-30oC or with ozone at temperature 15-25oC. The modified amino acid has no toxicity for health cells. Also, invention relates to a method for preparing such preparation. Invention provides the development of an antitumor preparation based on modified amino acids and expanded assortment of antitumor preparations being without cytotoxicity for normal cells.
EFFECT: valuable medicinal antitumor properties of preparation.
8 cl, 4 tbl, 2 dwg, 4 ex