Method for surgical approach to rat's shoulder blade experimentally

FIELD: medicine.

SUBSTANCE: general anaesthesia is performed. A skin incision 1.5-2 cm long is made with incising the skin, subcutaneous fat and fascia. The muscles attached to an infraspinous fossa of shoulder bone are skeletonised towards a spine; a caudal surface of a scapular spine is skeletonised; a blind bone bed is formed within the scapular spine at an angle of 45° to the surface of infraspinous fossa of shoulder bone. An implant of the analysed material is implanted into the bone bed to achieve primary stability. The wound is closed.

EFFECT: method provides minimum injuries accompanying the wound formation, reduces a risk of the incision wound damage by an animal, makes it possible to perform the surgery on opposite shoulder blade simultaneously, ensures the adequate volume and quality of the bone tissue in the surgical site, prevents the disordered muscular integrity throughout and loss of no more than 1 point of the muscular attachment.

2 dwg, 2 ex

 

The invention relates to medicine, namely to experimental surgery, pathological anatomy and pathological physiology. Various methods of surgical access to the shoulder blade in the experiment on rats to evaluate the osseointegration of materials.

The known method of surgical access in the experiment on rats to evaluate the osseointegration of materials, which consists in the following steps. Under anesthesia to improve access to the alveolar bone and the teeth of the lower jaw hold an incision of soft tissues of the buccal region of the angle of the mouth 2 cm long After tooth extraction the lower jaw begin the formation of the bone bed for the implant with the use of generally accepted principles of atraumatic preparation of bone tissue. Drill diameter is 0.8 mm dissect a channel in the bone to a depth corresponding to the height of the intraosseous element, then set the implant in the generated box. Produce suturing of soft tissues [1].

The disadvantage of this method is to create trauma the wound, the risk of possible damage by animals of the postoperative wound, the risk of infection of the wound microflora of the oral cavity, insufficient volume and quality of bone tissue in the operated area.

The known method of surgical access in the experiment on rats to evaluate the osseointegration of materials, comprising �doing implants in the parietal-occipital area of subperiosteal [2].

The disadvantage of this method is to create trauma the wound, the risk of infection of the wound microflora of the oral cavity, the impossibility of studying the influence of osseointegration with spongy bone tissue.

The known method of surgical access to the shoulder blade in the experiment on rats to evaluate the osseointegration of materials, which consists in the following steps. Under anesthesia, make a removal of hair in the region of shoulder bones. Produce antiseptic treatment of the surgical field. Make a skin incision with a length of 1.5-2 cm in the projection of the spine of scapula, lateral to the spine. Layers cut through the skin, subcutaneous fat, fascia. The muscles in the spine of the scapula cut over and sceletium. In the projection of the spine of the scapula to form the bone defect. In formed bone defect was placed a sample of the studied material. The wound is sutured in layers [3].

The disadvantage of this method is the insufficient amount and quality of bone tissue in the operated area, poor fixation of the implant, the violation of the continuity of the muscles during loss of several points of attachment of muscles.

This method is taken as a prototype.

The aim of the invention is to ensure the minimal trauma of the creation of the wound, reducing the risk of damage of the animals of the postoperative wound, castaneocoronata to operate simultaneously on opposite bones, ensuring adequate quantity and quality of bone tissue in the operated area, a sufficient fixation of the implant, prevention of discontinuities of the muscles on the stretch, preventing the loss of no more than 1 point of attachment of muscles.

This object is achieved in that an incision of tissues in the projection of the lower edge of the infraspinatus fossa of the scapula, repeating its contours; stupidly pass into the region of the lower edge of the infraspinatus fossa of the scapula; produce stellerovaya muscles that attach to the infraspinatus fossa of the scapula towards the spine; sceletium caudal surface of spine of scapula; forming blind bone bed in the field of spine of scapula at an angle of 45° to the surface of the infraspinatus fossa of the scapula; the bone bed set the implant material to the primary stability.

The method is illustrated graphic material. Figure 1 formed blind bone bed in the field of spine of scapula at an angle of 45° to the surface of the infraspinatus fossa of the scapula; figure 2 shows the installed implant material in bone bed up to primary stability.

Method is used as follows. Under General, make a removal of hair in the area of the scapula rats. Produce antiseptic treatment of the surgical field. Produce �asres skin (2 cm in the projection of the lower edge of the infraspinatus fossa of the scapula, repeating its contours. Stupid pass into the region of the lower edge of the infraspinatus fossa of the scapula. Produce skalierbare muscles attach to the infraspinatus fossa of the scapula towards the spine. Skeletonize the caudal surface of the spine of the scapula. Form blind bone bed in the field of spine of scapula at an angle of 45° to the surface of the infraspinatus fossa of the scapula. In a bone bed set the implant material to the primary stability. The wound is sutured in layers. At the stage of osseointegration animal is withdrawn from the experiment. Is the fixation of drugs, decalcification. Study the osseointegration of the material with a spongy and compact bone tissue.

When the proposed method not violated the integrity of the muscles that attach to the scapula, over, through incision the lower edge of the infraspinatus fossa of the scapula, repeating its contours. When skeletron muscles that attach to the infraspinatus fossa of the scapula towards the spine, there is a loss of no more than 1 point of attachment of muscles to the scapula; fixation of the muscles to the spine and other bones of the thoracic limb is preserved [4].

Achieve the result of the new method is confirmed by examples, produced on the basis of the Institute of experimental medicine and biotechnology, Samara state medical Univ�sity.

The method presents the following examples.

Example 1. From the vivarium taken rat-male Wistar weighing 250 g. Under General anesthesia (Zoletil - Rometer) removes hairline in the area of the scapula rats. Produced antiseptic treatment of the surgical field. Produced skin incision (2 cm in the projection of the lower edge of the infraspinatus fossa of the scapula, repeating its contours. Stupidly passed into the region of the lower edge of the infraspinatus fossa of the scapula. Produced skalierbare muscles that attach to the infraspinatus fossa of the scapula towards the spine. Skeletron caudal surface of spine of scapula. Formed blind bone bed in the field of spine of scapula at an angle of 45° to the surface of the infraspinatus fossa of the scapula. In the bone cavity an implant was studied material to primary stability. The wound is sutured in layers. The animal alive and transferred to the vivarium. At 4 months after surgery, the animal is derived from the experiment. Produced by fixation of the drug in 12% neutral formalin for 2 days. After fixation, the material is washed in running water for 24 hours. The drug decalcified in a solution of Trilon-B for 4 weeks. Degreasing and dehydration of the material carried out in ethanol solutions (C2H5OH) increasing concentration(70°, 80°, 96° � absolute alcohol for 2-3 h in each solution), with further casting in paraffin. Slices with a thickness of 5 μm is made on a rotary microtome Sakura with subsequent staining with hematoxylin and eosin and pikrofuksin by van gieson. Investigated the osseointegration of the material with a spongy and compact bone tissue.

Example 2. From the vivarium taken rat-male Wistar weighing 200 g Under General anesthesia (Zoletil - Rometer) removes hairline in the area of the scapula rats. Produced antiseptic treatment of the surgical field. Made a skin incision of 1.5 cm in length in the projection of the lower edge of the infraspinatus fossa of the scapula, repeating its contours. Stupidly passed into the region of the lower edge of the infraspinatus fossa of the scapula. Produced skalierbare muscles that attach to the infraspinatus fossa of the scapula towards the spine. Skeletron caudal surface of spine of scapula. Formed blind bone bed in the field of spine of scapula at an angle of 45° to the surface of the infraspinatus fossa of the scapula. In the bone cavity an implant was studied material to primary stability. The wound is sutured in layers. The animal alive and transferred to the vivarium. On the 6th month after the operation the animal is derived from the experiment. Produced by fixation of the drug in 12% neutral formalin for 2 days. After fixation, the material is washed in running water for 24 hours. The drug calcinaro�h in a solution of Trilon-B for 4 weeks. Degreasing and dehydration of the material carried out in ethanol solutions (C2H5OH) of increasing concentration (70°, 80°, 96°, and absolute alcohol for 2-3 h in each solution), with further casting in paraffin. Slices with a thickness of 5 μm is made on a rotary microtome Sakura with subsequent staining with hematoxylin and eosin and pikrofuksin by van gieson. Investigated the osseointegration of the material with a spongy and compact bone tissue.

The positive effect of the proposed method is that it provides minimal trauma when creating the wound, reduces the risk of damage by animals of the postoperative wound, creates opportunities to operate simultaneously on opposite shoulder bones, provides sufficient volume and quality of bone tissue in the operated area, prevents discontinuities muscle for and loss is not more than 1 point of attachment of muscles. The method can be applied and widely used in experimental medicine to study the effects of new composite materials on the process of osseointegration.

Sources of information

1. Vorob'ev A. A., Mikhalchenko V. F., A. V. Poroshin, Mikhalchenko D. V., K. I. Hodes Possibilities of research of influence of TES therapy on the process of osseointegration // Bulletin of new medical technologies, 2012. - No. 1.

2. Malysheva N. M. Aagaard�of gnekow B. V. Evaluation of the effectiveness of the use of porous alumina ceramics for removing defects in the maxillofacial region // Dentistry, 2008. - No. 1.

3. Beglaryan V. V. Clinical and experimental rationale for the use of dental implants with a through porosity: Diss. Cand. honey. Sciences. - Samara. - 2011.

4. Nozdrachev A. D., Polyakov E. L. Anatomy of the rat. - 2001. - S. 36-77.

Method of surgical access to the shoulder blade in the experiment on rats to evaluate the osseointegration of the material, including General anesthesia, a skin incision with a length of 1.5-2 cm, dissection of the skin, subcutaneous fat, fascia; stellerovaya muscles in the field of spine of scapula; placement in a bone bed for the implant of the studied material; wound closure, characterized in that an incision of tissues in the projection of the lower edge of the infraspinatus fossa of the scapula, repeating its contours; stupidly pass into the region of the lower edge of the infraspinatus fossa of the scapula; produce stellerovaya muscles that attach to the infraspinatus fossa of the scapula towards the spine; skeletonize caudal surface of spine of scapula; forming blind bone bed in the field of spine of scapula at an angle of 45° to the surface of the infraspinatus fossa of the scapula; the bone bed set the implant material to the primary stability.



 

Same patents:

FIELD: medicine.

SUBSTANCE: method of the express-modelling wear of a polyethylene insert of a metal cup or a polyethylene cup under dynamic conditions at different angles of horizontal inclination in an experimental module of the hip joint endoprosthesis consists in carrying out long-term and multiple cyclic movements in the hip joint endoprosthesis module under experiment conditions. The experimental module of the hip joint endoprosthesis, which has a leg, a polyethylene insert of the metal cup or the polyethylene cup and a head, consisting of ceramics, metal, metal alloys and other materials with a rough surface, is used in the method. The experimental module of the hip joint endoprosthesis is fixed in the device in such a way that the head is located in the polyethylene cup or in the polyethylene insert of the metal cup of the endoprosthesis module, after which the device with the polyethylene insert of the metal cup or the polyethylene cup is installed in such a way that the longitudinal axis of the module head and neck has a 10-degree deviation relative to the plane of entrance into the polyethylene insert of the metal cup or into the polyethylene cup. After that dynamic tests for joined compression and cyclic twisting in the friction pair are carried out. Then, the worn polyethylene cup or the polyethylene insert and the head with the rough surface are replaced with new ones. The device with the new polyethylene insert of the metal cup or the polyethylene cup is installed in such a way that the longitudinal axis of the module head and neck has a 20-degree deviation relative to the plane of entrance into the polyethylene insert of the metal cup or into the polyethylene cup, after which dynamic tests for joined compression and cyclic twisting in the friction pair are carried out, after which the worn polyethylene cup or the polyethylene insert and the head with the rough surface are replaced with new ones. Then the device with the new polyethylene insert of the metal cup or the polyethylene cup is installed in such a way that the longitudinal axis of the module head and neck has a 30-degree deviation relative to the plane of entrance into the polyethylene insert of the metal cup or into the polyethylene cup, after which dynamic tests for joined compression and cyclic twisting in the friction pair are carried out, after which the wear of the polyethylene insert of the cup or the polyethylene cup of the experimental module of the hip joint endoprosthesis is analysed.

EFFECT: efficiency under dynamic conditions.

16 dwg

FIELD: medicine.

SUBSTANCE: invention refers to systems for training the medical stuff and population to get skills of the first aid to an individual suffering from the upper airway obstruction with a foreign matter. An interactive training system comprises a full-size model of the human body including a head unit with the oral cavity and an open mouth provided with a foreign matter simulator, the neck unit with anelastic deformation front surface connected to the head unit through the neck unit, and a device for the foreign matter extraction from the upper airway. The model is made of an inflexible plastic material and provided with an electronic assembly, an upper airway obstruction simulator, a sensor of forward and backward flexion of the head, a sensor of a paramedic's hands position on the chest surface when giving a closed-chest cardiac massage, a sensor of the paramedic's hands position on the abdominal surface, a sensor of back strokes, a sensor of forward inclination of the body connected to the electronic assembly which is also connected to a control unit, dynamic speakers and an internal battery.

EFFECT: training system enables simulating pathological conditions occurred in the choking individual, extending the range of trained rescuing measures, improving the ease of use and enabling the self-assisted training of the rescue skills.

8 cl, 3 dwg

FIELD: medicine.

SUBSTANCE: general anaesthesia is performed. A skin incision 1.5-2 cm long in a projection of the shoulder bone is performed; the skin, subcutaneous fat and fascias are excised; muscles are skeletonised within the scapular spine. An examined material is implanted into the bone bed, and the wound is closed. The muscles of the scapular spine are skeletonised in the ventral direction towards the glenoid cavity of the scapula. The neck of the scapula is skeletonised; a bone bed is formed within the neck of the scapula at 30°-45° to the surface of the neck of the scapula at a depth stop short of the wall of the glenoid cavity. The examined material is implanted into the bone bed of the neck of the scapula until primarily stabilised.

EFFECT: method provides the minimum injuries accompanying the wound formation, reduces the risk of a postoperative wound damage by animals, makes it possible to perform surgical interventions on both scapulas simultaneously, provides an adequate volume and quality of the bone tissue in the incision area for the purpose of studying osteointegration processes, and ensures the adequate fixation of the implant.

2 ex, 1 dwg

FIELD: medicine.

SUBSTANCE: method involves general anaesthesia, tissue dissection, lower jaw skeletisation, defect creation, and wound closure. The skin is incised in a projection of a lower edge of the lower jaw within the vascular segment at 5 mm from the edge of the lower jaw. The incision extends by blunt dissection towards the lower edge of the lower jaw with the facial vein and artery towards the mandibular angle; the lower edge of the lower jaw is skeletonised within the vascular segment. A blind vascular bed is formed within the vascular segment or at 1-5 mm anteriad from the vascular segment at an angle of 40-75° to the lower edge of the lower jaw in parallel with the mandibular canal; an implant made of the investigated material is placed into the bone bed until primarily stabilised.

EFFECT: method provides the minimum injuries accompanying creating the wound, reduces the risk of the incision wound damage by animals, enables performing the operation on both sides of the skeleton simultaneously, provides an adequate amount and quality of the bone tissue in the surgical area, reduces a risk of involving vital anatomical regions, and preserves the functional and anatomical integrity of the mastication muscles.

2 ex, 6 dwg

FIELD: medicine.

SUBSTANCE: stellaria grass infusion is introduced to laboratory animals in a dose of 5 ml/kg of weight 20 minutes before irradiation in an ultraviolet chamber for 14 days daily.

EFFECT: stable pharmacological effect under conditions of reduction of the phytocorrection course duration.

3 tbl

FIELD: medicine.

SUBSTANCE: invention refers to experimental medicine and concerns simulating a bite wound. That is ensured by forming an experimental animal's skin wound by an instrument similar to a bite with small mammal's upper and lower jaws. Fresh saliva with oral microflora taken from some mammalian donors is introduced into each wound. The saliva is taken in an equal volume in each donor, and the saliva mixture is introduced into the bite wound in an equal dose to the animals of all experimental groups.

EFFECT: method provides higher reliability of the comparative analysis of the experimental study results by the more accurate creation of the simulated bite wound of the same length, depth and microfloral contamination in the various experimental groups in the animals.

FIELD: radio engineering, communication.

SUBSTANCE: mobile computer simulator for public civil defence training includes a vehicle, a mobile power generating unit with an electric wire unit, a built-in room with training places for public training, simulators, simulator robots and means for accommodating trainees. The vehicle has three compact training places. The first is a lecture hall for theoretical classes and testing based on a self-contained pneumatic-frame Emergencies Ministry tent. The second training place is a simulator for processing practical skills of the public during emergency situations, with a simulator robot for processing first aid exercises during emergency situations. The third training place is a simulator with a set of different equipment of protecting respiratory organs. Practical skills on offering first aid and transporting victims are processed using equipment and video materials for practical training.

EFFECT: high efficiency of the public training system and high moral and psychological state of the public in times of danger.

3 cl, 4 dwg

FIELD: medicine.

SUBSTANCE: method for designing a bioengineered rat's lung frame involves lung perfusion by detergent-enzymatic method with controlling the frame quality by histological method. The lung perfusion is accompanied by continuous atmospheric air pulmonary ventilation according to physiological parameters, through a trachea for 24 hours by means of successive exposure to decellularising solutions. That is ensured by using phosphate buffer, 1% aqueous sodium deoxycholate, swine pancreatic DNAase I, purified water at an equal exposure length. To ensure the quality of the following re-cellularisation, a colorimetric method is used to prove the designed lung frame biocompatible, viable and having its architechtonics unchanged by determining its biomechanical tensile and compression strength, by fixing pulmonary compliance.

EFFECT: maintaining the lung matrix structure and its quality, eliminating the risk of contamination.

1 tbl

FIELD: medicine.

SUBSTANCE: laboratory rats with one shin bone excised are daily placed for 60 minutes into an altitude chamber in the environment of 1.5 absolute atmospheres. The therapeutic course makes 10 sessions.

EFFECT: method optimises the articular cartilage condition, improves its morphological structure, delays osteoporosis and provides their partial regression.

1 tbl

FIELD: medicine.

SUBSTANCE: skin graft is simulated in laboratory animals on the second experimental day. Dihydroquercetin is administered intragastrically in a daily dose of 5.5 mg/kg from the first day every 46 hours of the experiment.

EFFECT: increasing the skin graft survival in the reduced circulation environment by activating the pre-conditioning process.

1 ex, 1 tbl

FIELD: medicine, experimental abdominal surgery.

SUBSTANCE: as experimental animals one should apply mongrel dogs of 12-17 kg body weight. Under general anesthesia one should conduct superior-median laparotomy, introduce 3.0 ml 70%-ethanol solution under pancreatic capsule and then laparotomic wound should be sutured up. Manipulation should be performed once. The method provides modeling adequate acute pancreatic inflammation at no side effects being very simple in implementation.

EFFECT: higher efficiency.

3 dwg

FIELD: medicine; medical engineering.

SUBSTANCE: method involves studying transverse longitudinal and rotation stiffness characteristics. The studies are carried out step-by-step from the first order units to complete external fixation apparatus structure. The device has frame and is provided with calibration loads, wire rope, displacement indicators, strip for fastening to loading end of bone imitator fragment, beam for fixing displacement indicators, beams having unit for modeling longitudinal and transverse loadings. The frame is manufactured as parallelepiped. The fixing panel has openings for bone imitator, for fixing external fixation apparatus and yoke connection union and is fixed in end face part of the frame. Beam for fixing displacement indicators has longitudinal slit for fixing the indicators and arranging them on lateral slots in frame base. The beams having unit for modeling rotational, longitudinal and transverse loadings are arranged on lateral frame sides on lateral slots in base.

EFFECT: high vision acuity without applying spectacle-based correction; accelerated treatment course.

2 cl, 16 dwg, 1 tbl

FIELD: experimental medicine.

SUBSTANCE: the present innovation deals with modeling urinary calculosis in rats due to injecting intraperitoneally 60%-glucose solution at 1 ml/100 g animal body weight twice daily for 2 mo. The method is very simple and enables to achieve lithogenesis in 25% experimental animals.

EFFECT: higher efficiency of experimental modeling.

2 dwg, 2 ex, 4 tbl

FIELD: medicine.

SUBSTANCE: method involves using Hann diode crystal with proper frequencies of pathogenic microorganisms and cells during their death period or during the stimulating factors action period being applied.

EFFECT: enhanced effectiveness of treatment; wider range of biophysical action types.

3 cl, 1 tbl

FIELD: medicine, experimental physiology.

SUBSTANCE: hypoxia with hypercapnia should be modeled due to creating a closed system of inhaled air circulation. Air enters lungs out of hermetically sealed reservoir and at expiration returns back. The process of recirculation is supplied with an apparatus of artificial pulmonary ventilation. The innovation suggested provides steadiness in development of hypoxia with hypercapnia excluding the development of stressor reaction. Conditions should be created to carry out any manipulations with an animal in the course of an experiment.

EFFECT: higher efficiency of modeling.

5 dwg, 1 ex

FIELD: medicine, stomatology.

SUBSTANCE: the present innovation should be carried out for the purpose to study ethiology and pathogenesis of parodontitis. One should affect with emotional stress in experimental animals (mature rats) due to placing 10-11 experimental animals into the cage at area of 0.018 sq. cm/animal. Before placing into the cage one should create artificial dental plaque around the cervix of the upper and lower incisors with the help of stomatological cement for every experimental animal. In the course of modeling all experimental animals should eat paste-like food. The method enables to shorten terms for obtaining the model desired and increase its similarity with pathomorphological manifestations of human parodontitis.

EFFECT: higher efficiency of investigation.

2 ex

FIELD: medical equipment.

SUBSTANCE: device has input first variable resistor, capacitor and permanent resistor. Permanent resistor is connected to arm second and third variable resistors. Second ends of variable resistors are connected with motionless contacts of polarized relay. Movable contact of relay is connected to common bus. Input of device is connected to amplifier which has output connected with control wiring of polarized relay. Second end of wiring is connected with common bus. Device is intended for electrical modeling of balanced and misbalanced conditions of acupuncture point at electropunctural action with unlike-poled signals due to liquidation mutual errors at any circuit of opposite arms of the device. Values of active resistances can be installed independently at any arm of device.

EFFECT: increased precision.

2 dwg

FIELD: experimental medicine.

SUBSTANCE: laboratory animals should be once injected intraperitoneally or intravenously with phenylhydrazine at the dosage of 100-150 mg/kg.

EFFECT: higher efficiency.

1 cl, 4 ex, 2 tbl

FIELD: medicine, experimental biology, ecology, toxicology.

SUBSTANCE: at studying the mechanisms of heavy metals toxic action, in particular, cadmium upon renal function, it is suggested to introduce cadmium sulfate solution into stomach once daily for 2 mo at the dosage of 0.5 mg/kg, on conversion to metal, where cadmium corresponds to 0.5 mg per 1 ml solution. The present innovation enables to study the pathology in dynamics of development and elaborate and searching preparations for treating and preventing chronic toxic nephropathy.

EFFECT: higher efficiency.

1 ex, 3 tbl

FIELD: medicine.

SUBSTANCE: method involves exposing cell or cell group to external power source. At least two electrodes are introduced before treating the cells. One of electrodes is set on cytoplasmatic external cell membrane surface and the other one cell membrane and membrane potential value is measured. External electric voltage source is connected to the introduced electrodes oppositely in polarity with cell membrane potential difference value being not less than cell membrane potential.

EFFECT: enhanced effectiveness in building cell damage model by means of energy burst and death.

Up!