Modified method for experimental surgical approach to rat's shoulder blade

FIELD: medicine.

SUBSTANCE: general anaesthesia is performed. A skin incision 1.5-2 cm long in a projection of the shoulder bone is performed; the skin, subcutaneous fat and fascias are excised; muscles are skeletonised within the scapular spine. An examined material is implanted into the bone bed, and the wound is closed. The muscles of the scapular spine are skeletonised in the ventral direction towards the glenoid cavity of the scapula. The neck of the scapula is skeletonised; a bone bed is formed within the neck of the scapula at 30°-45° to the surface of the neck of the scapula at a depth stop short of the wall of the glenoid cavity. The examined material is implanted into the bone bed of the neck of the scapula until primarily stabilised.

EFFECT: method provides the minimum injuries accompanying the wound formation, reduces the risk of a postoperative wound damage by animals, makes it possible to perform surgical interventions on both scapulas simultaneously, provides an adequate volume and quality of the bone tissue in the incision area for the purpose of studying osteointegration processes, and ensures the adequate fixation of the implant.

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The invention relates to medicine, namely to experimental surgery, pathological anatomy and pathological physiology. Various methods of surgical access in the experiment for the study of osseointegration.

The known method of surgical access in the experiment on rats to evaluate the osseointegration of materials, which consists in the following steps. Under General anesthesia on the back of the thigh to produce a linear skin incision, blunt pushing muscles, bare thigh and fissure Bur at low speed with sterile water cooling form a longitudinal hole in the middle of the diaphysis length of about 5 mm. Into the hole install the titanium implant, the skin and muscles laid in place and covered with silk sutures [1].

The disadvantage of this method is to create trauma the wound, the risk of possible damage by animals of the postoperative wound, infringement of function of a limb of an animal, the inability to operate simultaneously on opposite bones, insufficient volume and quality of bone tissue in the operated area.

The known method of surgical access in the experiment on rats to evaluate the osseointegration of materials, which consists in the following steps. Under General anesthesia an incision of skin and subcutaneous fat.�the front surface of the left thigh length 1.5 cm. The wound edges are mobilizing, longitudinally cut through the muscle layer and the periosteum, dissect the anterior surface of the femur in the region of the diaphysis. With the help of a drill spherical boron produce longitudinal propyl cortical plate and cancellous bone length 10 mm, width 0.55 mm, a depth of 0.55 mm. obtained In the bone cavity implanted sterile sample. The implant is fixed to the femur 2 catgut ligatures. The wound is sutured in layers with interrupted sutures, completely covering the implant [2].

The disadvantage of this method is to create trauma the wound, the risk of possible damage by animals of the postoperative wound, infringement of function of a limb of an animal, the inability to operate simultaneously on opposite bones, insufficient volume and quality of bone tissue in the operated area.

The known method of surgical access to the shoulder blade in the experiment on rats to evaluate the osseointegration of materials which consists in the following steps. Under anesthesia, make a removal of hair in the region of shoulder bones. Produce antiseptic treatment of the surgical field. Make a skin incision with a length of 1.5-2 cm in the projection of the spine of scapula, lateral spine. Layers cut through the skin, subcutaneous fat, fascia. The muscles in the spine of the scapula cut for sceletium. In the projection of the spine of the scapula to form the bone defect. In formed bone defect was placed a sample of the studied material. The wound is sutured in layers [3].

The disadvantage of this method is the insufficient amount and quality of bone tissue in the operated area, poor implant fixation.

This method is taken as a prototype.

The aim of the invention is to ensure the minimal trauma of the creation of the wound, reducing the risk of damage of the animals of the postoperative wound, creating opportunities to operate simultaneously on opposite bones, ensuring sufficient volume and quality of bone tissue in the operated area for the study of osseointegration, ensuring adequate fixation of the implant.

This object is achieved in that produce stellerovaya muscle spine of scapula in the ventral direction, in the direction of the glenoid cavity of the scapula; skeletonize the neck of the scapula; forming a bone bed in the neck of the scapula the scapula at an angle of 30°-45° relative to the surface of the neck of the scapula, with a depth not reaching the walls of the glenoid cavity; in the bone cavity of the neck of the scapula set the implant material to the primary stability.

The method is illustrated graphic material.

Figure 1 from�affected scapular bone (1) rats. In the neck (2) of the scapula formed bone bed (3) at an angle of 30°-45° relative to the surface of the cervix (2) of the scapula, with a depth not reaching the walls of the glenoid cavity.

Method is used as follows.

Under General anesthesia, make a removal of hair in the area of the scapula rats. Produce antiseptic treatment of the surgical field. Make a skin incision with a length of 1.5-2 cm in the projection of the spine of the scapula, cutting the skin, subcutaneous fat, fascia. Skeletonize the muscles in the spine of the scapula in the ventral direction, in the direction of the glenoid cavity of the scapula. Skeletonize the neck of the scapula. Form a bone bed in the neck of the scapula the scapula at an angle of 30° relative to the surface of the neck of the scapula, with a depth not reaching the walls of the glenoid cavity. In the bone cavity of the neck of the scapula set the implant material to the primary stability. The wound is sutured in layers. At the stage of osseointegration animal is withdrawn from the experiment.

Is the fixation of drugs, decalcification. Study the osseointegration of the material with a spongy and compact bone tissue.

Bone in the neck of the scapula has developed spongy and compact substance, compared with the spine of scapula, h�about in the experiment allows to study the processes of osseointegration materials [4].

Achieve the result of the new method is confirmed by examples, produced on the basis of the Institute of experimental medicine and biotechnology, Samara state medical University.

The method presents the following examples.

Example 1. From the vivarium taken rat-male Wistar weighing 250 g. Under General anesthesia (Zoletil-Rometer) removes hairline in the area of the scapula rats. Produced antiseptic treatment of the surgical field. Produced skin incision with a length of 1.5-2 cm in the projection of the spine of the scapula, cut the skin, subcutaneous tissue, fascia. Skeletal the muscles in the spine of the scapula in the ventral direction, in the direction of the glenoid cavity of the scapula. Skeletron the neck of the scapula. Formed bone in the neck of the scapula the scapula at an angle of 30° relative to the surface of the neck of the scapula, with a depth not reaching the walls of the glenoid cavity. In the bone cavity of the neck of the scapula an implant was studied material to primary stability. The wound is sutured in layers. The animal alive and transferred to the vivarium. At 4 months after surgery produced by the removal of the animal from the experiment. Produced by fixation of the drug in 12% neutral formalin for 2 days. After fixing mate�ial washed in running water for 24 hours. The drug decalcified in a solution of Trilon-B for 4 weeks. Degreasing and dehydration of the material carried out in ethanol solutions (C2H5OH) of increasing concentration (70°, 80°, 96°, and absolute alcohol for 2-3 hours in each solution), with further casting in paraffin. Slices with a thickness of 5 μm is made on a rotary microtome Sakura with subsequent staining with hematoxylin and eosin and pikrofuksin by van gieson. Investigated the osseointegration of the material with a spongy and compact bone tissue.

Example 2. From the vivarium taken rat-male Wistar weighing 200 g Under General anesthesia (Zoletil-Rometer) removes hairline in the area of the scapula rats. Produced antiseptic treatment of the surgical field. Produced skin incision with a length of 1.5-2 cm in the projection of the spine of the scapula, cut the skin, subcutaneous tissue, fascia. Skeletal the muscles in the spine of the scapula in the ventral direction, in the direction of the glenoid cavity of the scapula. Skeletron the neck of the scapula. Formed bone in the neck of the scapula the scapula at an angle of 45° relative to the surface of scapula, long before reaching the wall of the glenoid cavity. In the bone cavity of the neck of the scapula an implant was studied material to primary stability. R�on sutured in layers. The animal alive and transferred to the vivarium. On the 6th month after the operation the animal is derived from the experiment. Produced by fixation of the drug in 12% neutral formalin for 2 days. After fixation, the material is washed in running water for 24 hours. The drug decalcified in a solution of Trilon-B for 4 weeks Degreasing and dehydration of the material carried out in ethanol solutions (C2N5IT) of increasing concentration (70°, 80°, 96°, and absolute alcohol for 2-3 h in each solution), with further casting in paraffin. Slices with a thickness of 5 μm is made on a rotary microtome Sakura with subsequent staining with hematoxylin and eosin and pikrofuksin by van gieson. Investigated the osseointegration of the material with a spongy and compact bone tissue.

The positive effect of the proposed method is that it provides minimal trauma when creating the wound, reduces the risk of damage by animals of the postoperative wound, creates opportunities to operate simultaneously on opposite shoulder bones, provides sufficient volume and quality of bone tissue in the operated area for the study of osseointegration provides adequate fixation of the implant.

The method can be applied and widely used in experimental medicine to study the effects of new comp�composite materials on the process of osseointegration.

SOURCES of INFORMATION

1. Malginov N. N. Improving the efficiency of osseointegration of titanium dental implants by optimizing their shape, surface texture and application of cellular technologies in the experiment: Diss. Dr. med. Sciences. - Moscow. - 2011.

2. Kulakov A. A., Grigoryan A. S., M. R. Filonov, Shtansky D. V., Toporkov, A. K. Experimental-morphological study of the integration of the hybrid implant material in bone tissue // Dentistry, 2009. - No. 3. - P. 8-12.

3. Beglaryan V. V. Clinical and experimental rationale for the use of dental implants with a through porosity: Diss. Cand. honey. Sciences. - Samara. - 2011.

4. Nozdrachev A. D., Polyakov E. L. Anatomy of the rat. - 2001. - S. 36-77.

A modified method of surgical access to the shoulder blade in the experiment on a rat, including General anesthesia, a skin incision with a length of 1.5-2 cm in the projection of the spine of the scapula, dissection of the skin, subcutaneous fat, fascia; stellerovaya muscles in the field of spine of scapula; placement in a bone bed for the implant of the studied material; wound closure, characterized in that produce stellerovaya muscle spine of scapula in the ventral direction, in the direction of the glenoid cavity of the scapula; skeletonize the neck of the scapula; form a bone bed in the neck of the scapula scapular coast� at an angle of 30°-45° relative to the surface of the neck of the scapula, depth before reaching the wall of the glenoid cavity; in the bone cavity of the neck of the scapula set the implant material to the primary stability.



 

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