Method of correcting processes of peroxidation of biomembrane lipids under conditions of ultraviolet irradiation
SUBSTANCE: stellaria grass infusion is introduced to laboratory animals in a dose of 5 ml/kg of weight 20 minutes before irradiation in an ultraviolet chamber for 14 days daily.
EFFECT: stable pharmacological effect under conditions of reduction of the phytocorrection course duration.
The invention relates to medicine, in particular to pharmacology, and can be used for correction of processes of lipid peroxidation of biomembranes and improving the antioxidant status of the warm-blooded organism in conditions of exposure to ultraviolet rays, to find application in experimental medicine and clinical practice.
Known methods of correction of processes of lipid peroxidation and increasing the antioxidant status of the warm-blooded organism in terms of exposure to prooxidant factors the introduction of synthetic drugs antioxidant action - dibunola, tocopherol acetate, Mexidol [1, Mashkovsky M. D. Medicines, 2010], emoxipin [2, V. A. dorovskikh, Antioxidants in the prevention and correction of cold stress, 2000]. The disadvantages of these methods are the need of the use of pharmacological drugs of synthetic origin, having a number of side and toxic effects and relatively high cost.
The known method of correction of lipid peroxidation processes in the experiment including the irradiation region of the xiphoid process of the sternum albino rats electromagnetic terahertz waves at frequencies of 150,176-150,664 GHz at a power density of 0.2 mW/cm2within 30 minutes [3, Patent RF №2393891]. The disadvantage of this method is �necessary special equipment for irradiation of electromagnetic waves.
Known way to increase nonspecific resistance of the organism in the conditions of UV irradiation, to ensure stabilization of processes of peroxide oxidation of lipids of membranes in the body irradiated animals, the introduction of infusion based on the collection of nettle leaves, birch, plantain, oak and flowers of tansy, in the ratio 1:1:1:1:1 [4, RF Patent №2485598]. The disadvantage of this method is multicomponent collect to prepare, providing an antioxidant effect.
Also known a method of improving the antioxidant status of the warm-blooded organism in the conditions of UV irradiation introduction of infusion based on the collection of nettle leaves, birch, plantain, taken in the ratio 1:1:1 [5, Patent RF №2424580]. This technical decision taken by us as a prototype.
The object of the present invention is to expand the Arsenal of tools that allows you to adjust the processes of lipid peroxidation in the background of increased activity of the antioxidant system of warm-blooded organism in the conditions of UV irradiation, based on the available domestic raw materials and increase persistent pharmacological effect in terms of reducing the rate of fotokorrektsii.
The task is solved by developing a new method of correction of peroxidation whether�species under conditions of UV irradiation introduction the infusion of herbs starwort (woodlice). Grass starwort widely spread on the territory of Russia, contains a wide spectrum of biologically active substances. Raw materials used for production, available technology for producing cost-effective, wide range of applications.
The invention consists in that in the method of correcting processes of lipid peroxidation under conditions of ultraviolet irradiation, comprising daily oral administration to laboratory animals the infusion rate of 5 ml/kg 20 minutes before exposure to UV rays, animals injected infusion of the herb starwort, obtained by maceration of 6 g of raw material per 200 ml of boiling water, for 14 days.
The implementation of the method. Experimental animals (rats or mice) in standard conditions of vivarium, 20 minutes before exposure in a UV chamber [6, Patent RF №2348079] administered daily by oral infusion of the herb starwort at the rate of 5 ml/kg for 14 days. On the 15th day of the experiment animals were killed by decapitation.
Infusion starwort grass. The starwort grass were crushed, poured boiling water at the rate of 6 g per 200 ml of water, insisted 60 minutes, filtered, the precipitate was removed, the infusion has cooled.
The results were taken into account by the ratio of the content of peroxidation products (lipid hydroperoxides, diene conjugates, malonic dial�of Egida), the main components of AOS (ceruloplasmin, vitamin E) and the activity of AOS enzymes (glucose-6-phosphate dehydrogenase, catalase) in the blood plasma of rats of the experimental group in comparison with intact animals, control groups and the group prototype at 14 and 28 days of the experiment, treated with standard parametric methods using t-student criterion.
Method made possible the correction of processes of lipid peroxidation under conditions of UV irradiation, based on the decrease in the content of products of radical character and lipid peroxides in the body irradiated rats and the increase of antioxidant activity, in terms of reducing the duration of the course of fotokorrektsii to 14 days in comparison with the prototype.
Investigated the content of peroxidation products in blood plasma of intact rats, control groups, group prototype and experimental animals at 14 and 28 days of the experiment (table 1).
As a result of the conducted researches the content of lipid hydroperoxides in the blood of control (irradiated) animals were significantly higher by 46.6% compared to intact rats (p<0.01), diene conjugates - 53,0% (p<0,05), malondialdehyde - 62.5% (p<0,001), indicating the increase in the intensity of peroxidation processes in the conditions of daily UVR on day 14 of EC�experiment. The level of lipid hydroperoxides in plasma of rats treated amid irradiation infusion of the herb starwort, significantly lower by 20.5% than the control (irradiated) group of animals (p<0.01), diene conjugates - 26.6% (p<0.01), malondialdehyde - by 18.5% (p<0,05). Comparing the results of studies of LPO products in blood of animals of the experimental group with the prototype, we can conclude that the proposed method has a more pronounced effect on the stabilization of the processes of peroxidation on day 14 of the experiment: the level of lipid hydroperoxides in plasma of experimental animals relative to rats of group-prototype of 10.0% is lower diene conjugates - by 16.4%, malondialdehyde - 14.6%.
On day 28 of the experiment, the content of peroxidation products in the blood of control (irradiated) animals were significantly higher in comparison with the intact group of rats: the level of lipid hydroperoxide - by 26.2% (p<0,05), diene conjugates - 28.5% (p<0.01), malondialdehyde - 40.5% (p<0,05). In the blood of animals of the experimental group, the content of lipid hydroperoxide lower by 12.7% relative to the control (p<0,05), diene conjugates - 23.9% (p<0,001), malondialdehyde - by 23.8%. Comparative evaluation of the stabilizing activity against accumulation of products of the radical character of infusion of herbs starwort (EC�experimental group) and infusion of nettle leaves, birch, plantain (prototype) showed practically identical results obtained on day 28 of the experiment.
The increase in the intensity of processes of peroxide oxidation of lipids of membranes under conditions of UV exposure is associated with reduced activity of AOS components in the blood of irradiated animals compared with intact rats on day 14 of the experiment (table 2): the level of ceruloplasmin in the blood of control (irradiated) animals lower by 28.2% (p<0.01), vitamin E - by 31.0% (p<0.01).
In the blood of experimental animals, the content of ceruloplasmin were significantly higher by 19.9% compared with the control group rats (p<0,05), the level of vitamin E - 19.1%) (p<0,05). Comparative evaluation of results of research activity of components of EPA in the blood of animals of the experimental group and the prototype at the end of the second week of the experiment shows a more pronounced antioxidant effect from the infusion of herbs starwort (the content of ceruloplasmin higher by 14.1%, vitamin E - 7.5%).
On day 28 of the experiment under conditions of UV irradiation (control) observed reduced levels of ceruloplasmin by 34.2% compared to the intact group (p<0,001), vitamin E - by 31.2% (p<0.01). In the blood of rats of the experimental group, the content of ceruloplasmin higher relative to control 9.7%, �of itamin E - by 24.9% (p<0,05). Analyzing the investigated parameters in the dynamics, it is important to note that the most persistent pharmacological effect (antioxidant) is registered to the end of the second week of the experiment.
The activity of antioxidant enzymes ultraviolet irradiation varies accordingly to the nature of the variability of the major components by the end of the second and fourth weeks of the experiment (table 3): the activity of glucose-6-phosphate dehydrogenase in the blood of control rats following a relatively intact group of 21.5% (day 14, p<0,05) and 17.0% (day 28, p<0,05), catalase - 34.4% and 31.6%, respectively (p<0.01). The activity of the enzyme AOC glucose-6-phosphate dehydrogenase in the blood of experimental group animals on day 14 of the experiment were significantly higher at 20% compared to control (p<0,05), catalase - 34.8% (p<0.01) and decreases slightly towards the end of the experience. Comparative efficacy of infusion of herbs starwort and infusion of nettle leaves, birch, plantain (prior art) shows a predominant activating influence on the state of antioxidant system in the inventive method, and in relation to enzyme activity - both at 14 and 28 day studies.
Thus, experimentally the stabilizing effect of the infusion of herbs starwort on the processes of peroxide oxidation of lepidosiren under conditions of ultraviolet irradiation, based on the decrease in the content of peroxidation products and increased activity of the major components of the antioxidant system in the blood of irradiated animals that give grounds to recommend infusion of the herb starwort to application for correction of processes of lipid peroxidation in the background ultraviolet radiation.
Overall, based on the obtained experimental results, the proposed method (injection of the infusion of herbs starwort) provides the reduced rate of fotokorrektsii peroxidation processes in the conditions of UV exposure for up to 14 days in comparison with the prototype, showing a more pronounced pharmacological (antioxidant) effect.
The technical result of the invention is to reduce the duration of the course of fotokorrektsii peroxidation processes up to 14 days in comparison with the prototype ultraviolet irradiation under conditions of oral administration of infusion starwort grass for their antioxidant activity.
Method of correction of processes of peroxide oxidation of lipids of membranes under conditions of ultraviolet irradiation, comprising daily oral administration to laboratory animals the infusion rate of 5 ml/kg 20 minutes before exposure to ultraviolet rays, characterized in that the infusion is produced by infusion of 6 g herb starwort n� 200 ml of boiling water for 60 minutes, the duration of the course of fotokorrektsii is 14 days.
SUBSTANCE: invention refers to experimental medicine and concerns simulating a bite wound. That is ensured by forming an experimental animal's skin wound by an instrument similar to a bite with small mammal's upper and lower jaws. Fresh saliva with oral microflora taken from some mammalian donors is introduced into each wound. The saliva is taken in an equal volume in each donor, and the saliva mixture is introduced into the bite wound in an equal dose to the animals of all experimental groups.
EFFECT: method provides higher reliability of the comparative analysis of the experimental study results by the more accurate creation of the simulated bite wound of the same length, depth and microfloral contamination in the various experimental groups in the animals.
FIELD: radio engineering, communication.
SUBSTANCE: mobile computer simulator for public civil defence training includes a vehicle, a mobile power generating unit with an electric wire unit, a built-in room with training places for public training, simulators, simulator robots and means for accommodating trainees. The vehicle has three compact training places. The first is a lecture hall for theoretical classes and testing based on a self-contained pneumatic-frame Emergencies Ministry tent. The second training place is a simulator for processing practical skills of the public during emergency situations, with a simulator robot for processing first aid exercises during emergency situations. The third training place is a simulator with a set of different equipment of protecting respiratory organs. Practical skills on offering first aid and transporting victims are processed using equipment and video materials for practical training.
EFFECT: high efficiency of the public training system and high moral and psychological state of the public in times of danger.
3 cl, 4 dwg
SUBSTANCE: method for designing a bioengineered rat's lung frame involves lung perfusion by detergent-enzymatic method with controlling the frame quality by histological method. The lung perfusion is accompanied by continuous atmospheric air pulmonary ventilation according to physiological parameters, through a trachea for 24 hours by means of successive exposure to decellularising solutions. That is ensured by using phosphate buffer, 1% aqueous sodium deoxycholate, swine pancreatic DNAase I, purified water at an equal exposure length. To ensure the quality of the following re-cellularisation, a colorimetric method is used to prove the designed lung frame biocompatible, viable and having its architechtonics unchanged by determining its biomechanical tensile and compression strength, by fixing pulmonary compliance.
EFFECT: maintaining the lung matrix structure and its quality, eliminating the risk of contamination.
SUBSTANCE: laboratory rats with one shin bone excised are daily placed for 60 minutes into an altitude chamber in the environment of 1.5 absolute atmospheres. The therapeutic course makes 10 sessions.
EFFECT: method optimises the articular cartilage condition, improves its morphological structure, delays osteoporosis and provides their partial regression.
SUBSTANCE: skin graft is simulated in laboratory animals on the second experimental day. Dihydroquercetin is administered intragastrically in a daily dose of 5.5 mg/kg from the first day every 46 hours of the experiment.
EFFECT: increasing the skin graft survival in the reduced circulation environment by activating the pre-conditioning process.
1 ex, 1 tbl
SUBSTANCE: method for mechanical simulation of an external rotation contracture of a hip joint in an experimental unit of a hip implant consists in using the experiment unit of a hip implant comprising a head fixed on a holder, a polyethylene insert of a metal cup or a polyethylene cup and a device for cup fixation. The head consists of ceramics or metal alloys and has a rough surface. A device for cup fixation enables changing the position of the polyethylene insert of the metal cup or the polyethylene cup. The head is clamped experimentally on a holder in any of two collets of a universal test machine. The device with the polyethylene insert of the metal cup or the polyethylene cup is mounted in the other collet of the universal test machine so that the head is immersed completely into the polyethylene insert of the metal cup or the polyethylene cup. The head of the module is mounted in the polyethylene insert of the metal cup or the polyethylene cup so that a long axis of the head is deviated from a plane of an input into the polyethylene cup or polyethylene insert of the metal cup of the module of the implant by 10 degrees that corresponds to the outer rotation contracture of the degree 1 hip joint. The head of the module is placed into the polyethylene insert of the metal cup or the polyethylene cup so that the long axis of the head is deviated from the plane of the input into the polyethylene cup or polyethylene insert of the metal cup of the module of the implant by 20 degrees that corresponds to the outer rotation contracture of the degree 2 hip joint. The head of the module is placed further into the polyethylene insert of the metal cup or the polyethylene cup so that the long axis of the head is deviated from the plane of the input into the polyethylene cup or polyethylene insert of the metal cup of the module of the implant by 30 degrees that corresponds to the outer rotation contracture of the degree 3 hip joint.
EFFECT: setting up the clinical-biomechanical correspondence between a horizontal inclination angle and a degree of the external rotation contracture of the experiment module of the hip joint for the purpose of extrapolating the obtained experimental data of polyethylene abrasive wear to the clinics for the purpose of early and remote results of endoprosthesis replacement in the patients with external rotation contracture of the hip joint.
SUBSTANCE: invention relates to modelling in medicine and can be applied for the anatomo-surgical modelling of an angle of the horizontal inclination in the human hip joint in experiment. The posterior Kocher-Langenbeck access to the hip joint is performed. The head and neck of the femur, edges of the acetabulum are exposed; a guide wire, which determines a line of the plane of entrance into the acetabulum in the horizontal plane of the hip joint, is laid from the posterior to anterior edge of the acetabulum. Osteotomy of the neck and head of the femur is performed in the horizontal plane. The guide wire is passed through the base of the greater trochanter apex in the outside-inward direction along the longitudinal axis of the neck and head of the femur on the horizontal plane of the osteotomised head and neck of the femur to the subchondral part of the medial edge of the femoral head. Rotation of the hip by the shin rotation is performed with the lower extremity being bent in the knee joint at 45° angle in such a way that the angle of crossing of the guide wires in the area of the hip joint when measured constitutes 66°.
EFFECT: increased efficiency of anatomo-surgical modelling.
SUBSTANCE: method involves laparotomy in Wistar rats with inserting a complex of spleen and pancreas into the wound. That is followed by cryodestruction of a portion of pancreas for 5 sec with the use of an applicator of the area of 0.6 cm2 cooled in liquid nitrogen for 40 sec. That is followed by immersing the complex of the pancreas and spleen into the limited cavity pre-formed of the abdominal external and internal oblique muscles. That is followed by introducing S.aureus microbial suspension 0.3 ml 1 billion into this cavity. Before closing the incisional wound, a fistula is attached to leaves of the external oblique muscles for the following administration of drug preparations into the formed cavity.
EFFECT: providing the controlled simulation of pancreonecrosis close to that developing in the clinical settings.
6 dwg, 4 tbl, 1 ex
SUBSTANCE: method for instant simulation of abrasive wear of a polyethylene insert of a metal cup or a polyethylene cup of an experiment module of a hip implant, which has a head with a leg, a polyethylene insert of a metal cup or a polyethylene cup and a cup clamping device. The head consists of ceramics or metal alloys and has a rough surface. The experiment module of the hip implant is clamped in a special device so that the device is placed into the polyethylene insert of the metal cup or into the polyethylene cup of the module of the implant; a long axis of the head and leg of the module of the implant are perpendicular to a plane of an input into the polyethylene cup or polyethylene insert of the metal cup of the module of the implant. That is followed by dynamic co-compression and cyclic torsion tests in a friction pair; the experiment is terminated by producing the abrasive wear of the polyethylene insert of the metal cup or the polyethylene cup.
EFFECT: invention makes it possible to study the abrasive wear of the polyethylene insert of the cup or the polyethylene cup of the experiment module of the hip implant with the long axis of the head and the axis of the leg of the experiment module arranged perpendicularly to the plane of the input into the polyethylene cup or polyethylene insert of the metal cup of the module of the implant.
SUBSTANCE: shin muscle ischemia is simulated in laboratory animals on the second experimental day by surgical removal of a portion of great vessels, including femoral, popliteal, anterior and posterior tibial arteries. The ischemia is corrected by the intragastric administration of dihydroquercetin in a daily dose of 5.5 mg/kg every 46 hours of the first 7 experimental days.
EFFECT: effective treatment of the skeletal muscle ischemia by stimulating neoangiogenesis that is proved by laser Doppler flowmetry and morphological analysis.
SUBSTANCE: invention refers to medicine and can be used for making a drug for removing subcutaneous fat. That is ensured by using a composition containing: at least one phospholipid, at least one glycyrrhizic acid or a glycyrrhizic acid salt, and wherein total content of phospholipids and glycyrrhizic acid or its salts makes 2-80 wt % and a weight ratio of phospholipids and glycyrrhizic acid or its salts makes from 30:1 to 0.5:1. The composition can additionally contain additives. As phospholipid, the composition contains animal or herbal phosphatidylcholine. The composition can also contain glycyrrhizic acid or potassium, sodium, ammonium or magnesium salt of glycyrrhizic acid. As an additive, the composition contains sugar, particularly glucose, or maltose, and/or their derivatives, mannitol, sorbitol or lactose. The composition contains phosphatidylcholine in a total amount of 15 to 98 wt %, preferentially 30 to 98 wt %, more preferentially 50 to 98 wt %, especially preferentially 75 to 98 wt %, and most preferentially 75 to 90 wt % of total content of phospholipids. The composition is used in the dry form, preferentially in the form of lyophilisate prepared by freezing and drying, or in the form of a solution. The composition can contain physiologically acceptable solvents, including water, normal saline, glucose solution, such monohydric alcohols, as ethanol, 2-propanol, n-propanol, such polyatomic alcohols, as glycerol and/or propane diol, such polyglycols, as polyethylene glycol and/or Miglyol, glycerol, formal, dimethylisosorbitol, natural and synthetic oils and/or esters. Diseases of subcutaneous fatty tissue can be particularly related to local fat maldistribution. Fatty tissue tumour decomposition and reduction can be also solved. The drug can be presented in the form of cream, ointment, gel, hydrogel, lotion, paste, powder or solution. Undesired unaesthetic and pathological local fat maldistribution involve lipoedema, adipose growth, abdominal adiposis, cellulites, pseudogynecomasty, "buffalo hump" in HIV-infected patients, panniculitis or nonspecific subcutaneous fat deposition. The preparation is administered by subcutaneous, intra-abdominal, intramuscular or intravenous injection. The administration is implemented by a method specified in a group consisting of ioophoresis, electroporetion and phonophoresis.
EFFECT: described invention is successfully used for the above purposes.
17 cl, 11 ex, 7 tbl, 11 dwg
SUBSTANCE: what is presented is using meso-tetra(3-pyridyl)bacteriochlorin of structural formula (I) as a near-infrared photosensitiser for a photodynamic therapy. Doses 1.0-2.5 mg/kg of the declared photosensitiser have provided 70-100% tumour growth inhibition, 80-131% increase in life expectancy and 25-100% animals' recovery by selective tumour collection and fast clearance.
EFFECT: high photoinduced activity on human tumour cells of various epithelial origin and high dose-dependent anti-tumour effectiveness in the animal with tumours of various origins.
5 dwg, 8 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: presented invention refers to immunology. There are disclosed versions of a dimer compound for forming a multimer capable to reproduce the effector function of aggregated IgG with identical monomers. Each monomer of the dimer comprises: a monomer of IgG2 link region or a monomer of isoleucine zipper, dimerising each of which forms a multimerising region, and at least Fc-domain monomer containing a link region, CH2 domain and CH3 domain of IgG1. What is described is a multimer compound capable to reproduce the effector function of aggregated IgG and containing two or more dimers. There are disclosed a method for changing the immune response using the dimer or multimer, as well as a multimer-based method of treating an inflammatory disease.
EFFECT: using the invention provides the new compounds capable to bind at least one FcR specified in a group consisting of: FcγRI, FcγRII, FcγRIII, FcγRIV, or their non-human version that can find application in medicine for IVIG substitution for treating a wide range of diseases, including the inflammatory and autoimmune diseases.
7 cl, 25 dwg, 5 tbl, 25 ex
FIELD: veterinary medicine.
SUBSTANCE: product comprises Lycopodium clavatum, Acidum arsenicosum, Phosphorus, Podophyllum peltatum, Thuja occidentalis, Echinacea purpurea, Silybum marianum, Selenocysteine, and the components are taken in the dilutions described below in the following ratio, in parts: Lycopodium clavatum ⌀=D1 0.004, Podophyllum peltatum ⌀ 0.003, Acidum arsenicosum ⌀=D2 0.0001, Phosphorus ⌀=D3 0.001, Thuja occidentalis ⌀ 30, Echinacea purpurea ⌀ 30, Silybum marianum ⌀ 60, Selenocysteine 0.2.
EFFECT: product has an effective stress-protective and growth-stimulating effect, it regulates the metabolism in young farm animals.
3 cl, 10 tbl, 1 ex
SUBSTANCE: method for producing an agent for stimulating body cells involving preparing a mixture of aqueous solution of selenious acid and PEG 400; that is followed by preparing a mixture of hydrazine hydrochloride and PEG 400; the prepared mixtures are combined; the solution is put to dialyse against distilled water; surplus of water is driven off; the produced solution is added with hexamethylene tetramine; pH is reduced to 7.2-7.4; the method is implemented in certain circumstances.
EFFECT: producing high-effective, ecologically safe agent by the synergism of colloidal selenium and hexamethylene tetramine on body cell stimulation.
1 dwg, 2 tbl, 4 ex
SUBSTANCE: invention relates to medicine and can be used for the treatment of radiation-thermal injury of an organism. For this purpose a single subcutaneous introduction of bifidumbacterin, irradiated by gamma-rays in a dose of 14.0 Gy, is carried out. Bifidumbacterin is introduced in a dose of 1.43·106 CFU/kg. After that, 10% hypericum oil is applied on the burnt region. Then a 10% hypericum cream is applied after 3-4 days.
EFFECT: method makes it possible to carry out the treatment of combined radiation-thermal injuries in an effective way with the application of available and cheap pharmacotherapeutic means.
1 tbl, 4 ex
SUBSTANCE: erythrocyte cell medium is added with an aqueous solution of sodium and potassium salts of humic acids prepared on brown coal of leonardite in a dose of 10.0 mg/kg. That is incubated at a temperature of 37°C for 40 minutes before treatment with acidic haemolytic.
EFFECT: invention enables normalising the cell membrane permeability and reducing the damaged cell count under acidic haemolytic.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to a new intensifier of the antitumour effect, which is a uracil derivative of the general formula (I) or its pharmaceutically acceptable salt. In the general formula (I) X represents a C1-5-alkylene group, and wherein one of methylene groups making an alkylene group is optionally substituted by an oxygen atom; R1 represents a hydrogen atom or a C1-6-alkyl group; R2 represents a hydrogen atom or a halogen atom; and R3 represents a C1-6-alkyl group, C2-6-alkenyl group, C3-6-cycloalkyl group, (C3-6-cycloalkyl)-C1-6-alkyl group, halogen-C1-6-alkyl group or a 5-6-merous saturated heterocyclic group with an oxygen atom as a heteroatom, a uracil derivative presented by the following formula (I). The invention also refers to a method for potentiating the antitumour action or a method of treating tumours, involving administering an effective amount of a combination of the above uracil derivative or its pharmaceutically acceptable salt and an antimetabolite in an effective amount. The antimetabolite represents an agent specified in 5-fluoruracil (5-FU), potassium tegafur/gimeracil/oteracil (TS-1), tegafur/uracil (UFT), capecitabin, 5-fluor-2'-deoxyuridine (FdUrd) and Pemetrexed.
EFFECT: preparing the intensifier of the antitumour effect.
18 cl, 10 dwg, 10 tbl, 67 ex
SUBSTANCE: invention relates to medicine, namely to methods of purification and health improvement of an organism. For this purpose therapeutic starvation for not fewer than 5 days in case of a 7-day programme and for not fewer than 7 days in case of a 9-day programme is carried out. The duration of a recovery period constitutes two days. Food intake is realised nine times per day each day both in the process of therapeutic starvation and in the recovery period. In the period of therapeutic starvation the first food intake includes bee products "APIGRANULES 2" in a dose of one teaspoon, "KHINAZI balm" in a dose of one teaspoon, "A-P-V" in a dose of one teaspoon, "APITOK" in a dose of one teaspoon, "Antihelm phyto" - one capsule and still mineral water - one glass. The second food intake includes a drink with ginger, lemon juice, garlic and mint, honey - one teaspoon and one glass of apple-carrot juice. The third food intake supposes an intake of bee products "APIGRANULES 2" in a dose of one teaspoon, "KHINAZI balm" in a dose of one teaspoon, "A-P-V" in a dose of one teaspoon, "Antihelm phyto" - one capsule and still mineral water - one glass. The fourth food intake includes a drink with ginger, lemon juice, garlic and mint, honey - one teaspoon and one glass of apple-carrot juice. The fifth food intake consists of tea black or green with ginger, one teaspoon of honey and one glass of apple-carrot juice. The sixth food intake consists of bee products "APIGRANULES 2" in a dose of one teaspoon, "A-P-V" in a dose of one teaspoon, "Antihelm phyto" - one capsule and one glass of still mineral water. The seventh food intake includes tea black or green with ginger, one teaspoon of honey and one glass of apple-carrot juice. The eighth food intake supposes an intake of tea black or green with ginger, one teaspoon of honey and one glass of apple-carrot juice. The ninth intake of food includes depressant tea, including mint grass, valerian root, fennel seeds, cumin seeds, epilobium or strawberry leaves and one glass of apple-carrot juice. In the recovery period in 1-st, 2-nd, 3-rd, 4-th and 6-th intakes of food the composition of products remains the same as in the process of starvation. For the fifth intake of food the patients take tea black or green with ginger, one teaspoon of honey and vegetable soup. For the seventh intake of food the patients take tea black or green with ginger, "MILK COCKTAIL WITH CHITOSAN". The eighth intake of food includes tea black or green with ginger, one teaspoon of honey, "APICAMPA" cereal. The ninth intake of food for the recovery period includes a baked apple, depressant tea. In addition, a number of procedures are carried out after each food intake during the period of therapeutic starvation and the recovery period. After the first food intake gymnastics "5 Tibetan pearls" is realised. After the second food intake exercises on training apparatuses, procedures of press-therapy, electrolypolysis and myostimulation are realised. After the third food intake exercises of therapeutic physical training or aerobics are performed. After the fourth food intake a rest in form of a walk, a halotherapy session, combined with a session of relaxation therapy are realised. After the fifth food intake a course of strip-plastic is carried out. After the sixth food intake massage by manual application with a peloid-based mixture or manual massage with honey is carried out. After the seventh food intake an infra-red sauna, shower, phytobath with medicinal herbs, honey, ginger, lemon juice is taken. After the eighth food intake a shower is taken.
EFFECT: method ensures effective health improvement of the organism with the preservation of the full value life style and a sense of a comfortable state in the starvation period, reduction of recovery period term after starvation, purification of the organism without loading on the gastrointestinal tract, weight loss, and improvement of the general state and workability of the patients.
3 tbl, 7 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to biotechnology, specifically to a fused protein containing a variant of rodostomin, and can be used in medicine. An ανβ3 integrin selective polypeptide consisting of an amino acid sequence SEQ ID NO:1 conjugated on the N terminal by a linker amino acid sequence containing a combination of the amino acids glycine and serine with a variant of a human serum albumin (HSA) with SEQ ID NO:4.
EFFECT: invention enables the higher therapeutic effectiveness in the diseases related to ανβ3 integrin.
12 cl, 14 dwg, 2 tbl, 7 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to the field of pharmaceutics and represents a composition for oral cavity care in the form of dental varnish, containing bioactive glass and fluoride, where the said fluoride is present in the composition in an amount of up to 5% by weight. An increased ionic release is observed for the dental varnish containing bioactive glass and fluoride.
EFFECT: invention provides the creation of compositions, containing bioactive glass, for which in case of applying fluoride in lower concentrations (lower than 900 mln-1) its efficiency is the same as for higher concentrations (900 - 1450 mln-1).
3 cl, 2 ex, 6 tbl