Method for simulating bite wound

FIELD: medicine.

SUBSTANCE: invention refers to experimental medicine and concerns simulating a bite wound. That is ensured by forming an experimental animal's skin wound by an instrument similar to a bite with small mammal's upper and lower jaws. Fresh saliva with oral microflora taken from some mammalian donors is introduced into each wound. The saliva is taken in an equal volume in each donor, and the saliva mixture is introduced into the bite wound in an equal dose to the animals of all experimental groups.

EFFECT: method provides higher reliability of the comparative analysis of the experimental study results by the more accurate creation of the simulated bite wound of the same length, depth and microfloral contamination in the various experimental groups in the animals.

 

The invention relates to experimental medicine and is intended for modelling small bite wounds in experiments on mammals.

With the increase in the number of bites people animals mammals [Old B. C., Potkin, T. N., Golovina, L. N., Agafonov, A. N. Emergency situation - the growth of injuries from animal bites. Man and his health. Eighth Russian national Congress, 24-28 November 2003. Saint-Petersburg. 2003 - P. 357-358], the relevance of the treatment of such patients is increasing. Not only heavy damage from the bites, but the shallow bite wounds require proper treatment to prevent infectious complications. Encountered in one bite in a single anatomical localization of several minor wounds from the teeth of small mammal animal, we believe it is possible to call the bitten wound. Multiple same injury called multiple injuries of different localizations of the same anatomical system of the body. Found that feature bite wounds, even if it is shallow, is the high level of pollution of its pathogenic microorganisms in the saliva of biting [Kollontai Yu. Yu., Panchenko, M. K., Andruson M. V. Open injuries of the hand. Kiev, "Health", 1983. P. 88]. It is known that in the bitten wounds often streptococci, staphylococci, Bacteroides and other anaerobes, anaerobic bacteria, gram-negative bacilli [Managing bites from humans and other mammals.DTB 2004; 42(9): 67-70; Treatment bitten wounds. Published: Wednesday, may 25, 2005 - 08:55] and other microflora in different climatic conditions and ecological environment [Potkin, T. N., Old B. C., B. C. Soft, Vdovchenko O. Y. Injuries from animal bites to conditions of Kuzbass. Bulletin of the Kuzbass research center. Issue 7. "Public health issues". Volume 1. Kemerovo. 2008. P. 189-190]. It is known that through the epithelium of the gingival papillae in interdental spaces do desquamated epithelial cells, leukocytes, together with microorganisms and their metabolic products, which can be a cause of infection of bite wounds. In the bitten wounds inflicted by different types of animals that may be not the same microflora, at this point a special saturation pathogenic microbial flora of human saliva. Even in the mouth of a healthy person 1 mm3dental plaque contains 100-200 million colonies of saprophytes and pathogens, both gram-positive and gram-negative cocci, spirochetes, Bacillus, actinomycetes, protozoa, fungi, etc. [N.F.Danilevsky, E. A. Meguid, N. And.Mukhin, V. Yu Milicevic. Of periodontal disease. Atlas. Under the editorship of Prof. N. And.Danilevsky. M., "Medical". 1993. C-41, 51, 64]. When not deep wounds from the bites of small animals that are not subject to surgical treatment�TKE, the resulting infection can lead to abscess, osteomyelitis, septic arthritis, meningitis, sepsis, and even rabies [Kollontai Yu. Yu., Panchenko, M. K., Andrusov M. V. 1983. Ibid.], which indicates the inefficiency of current treatment methods. The necessity of finding more efficient technology for the treatment of patients with small bites inflicted by animals mammals, previously motivated us to develop a new external agent (the Decision to grant a patent on the invention under the application №2011111925). To explore new ways to treat victims of stings, in particular the influence of the us invented the funds for a period of bite wounds and the condition of the body, there is a need of carrying out experiments with the formation of bite wounds. For the purity of the experiments and the validity of the studies of the wound should be the same not only on the localization, shape, size and depth, but the content of pathogenic organisms in each experience of the main and control groups of experiments. In the available literature, we have not met the method of forming bacterial contaminated bite wounds in experimental animals, which prompted us to develop ways of modelling bite wounds with a wide range of pathogenic microorganisms in it.

Known causing bite wounds one animal to another (e.g.�, in dog fights) that theoretically could be organized and in a scientific experiment.

However, in this method there is no ethical compulsion of one animal without his willingness to bite another animal. The disadvantage of the natural bite from another animal is also that the formation of the wound is not controlled by the experimenter. Bites inflicted without the control of the experimenter, will be different according to the localization, size and depth. The difference in the bites in animals of different groups leads to repetitive injuries, which is not conducive to the high purity of the experiment. In this method of forming the bitten wounds there are no signs of increase of reliability of results of the comparative analysis of experimental research in control and treatment group by a more precise formation of the bite wounds are equal in size, depth and contamination microflora in all groups of experiments on animals.

Known application of the wound covering of the body of the surgical instrument, for example a clip used for attaching surgical linen to the skin. The clip at the enlarged base of the jaws has a transverse row of teeth with a pointed apex, which at the closing of pierce the integument [kabatov Y. F. Medical instruments, apparatus and equipment. "Medicine". M., 1977. Page 118-119 Fig.22-G]. Such a tool can be traumatic movement to put some small wounds.

The disadvantage of this method is the lack of signs improve the reliability of the comparative analysis of the results of an experimental study by modeling the bite wounds, more similar to natural, applied to small animals, and the same contamination microflora in different groups of experiments.

The closest in technical essence to the proposed technical solution is the latest method that we adopted for the prototype, and his shortcomings set forth above.

The technical result of the invention is to improve the accuracy of comparative analysis of the results of an experimental study by more precise creation of simulated bite wounds, both were the same size, depth and contamination microflora in different groups of experiments on animals.

The technical result of the invention is achieved in that the method of simulation of bitten wound comprises applying one injury with the formation of several wounds cover his body. And the wound in experimental animal dosed form tool according to the mechanism of the bite of the teeth of the upper and lower jaws of small mammal, and in every wound bring the mixture of fresh saliva from the microphone�ora of the oral cavity, taken from several mammalian donor.

In a preferred embodiment of the saliva take in equal volume from each blood donor and injected a mixture of saliva in the bitten wound in the same dose of experimental animals in all groups of experiments.

A specific example of the method in the experiment. Previously from several mammalian donor saliva in equal amounts each donor and mixed for the formation of mixtures with a wide range of microorganisms. After immersion in anesthetized experimental animals (lab rats), on the outer surface of the middle third of his hips to grasp a fold of skin and teeth special cutters, like the bite of the teeth of the upper and lower jaws of small mammal, inflict injury with the formation of several wounds on opposite sides of the skin fold. Then in each wound make a mixture of fresh saliva from the oral cavity microflora taken from several mammalian donor. Also simulate bacterial contaminated bitten wound the other experimental animals in other groups of experiments.

The significance of differences of the claimed method from the selected prototype is as follows. It is known that externally wound is characterized by three main local signs: the size, depth and localization (Struchkov V. I. General surgery, M., 1966). The formation�the stripes, the wounds in experimental animal rows of teeth of the tool, which the gnashing pierce the skin on the mechanism of the bite of the teeth of the upper and lower jaws of small mammal, is allowed the experimenter or under his control in all experimental animals dosed to form the wound, identical in size and depth. Since bitten, the wound has a high level of contamination of pathogenic microorganisms, the implementation of sampling saliva from several donor animal in equal amounts each donor, provides the same opportunity of contamination microflora wounds in all experimental animals in different groups and, in addition, if necessary, to repeat exactly the amount of re-sampling of saliva and the formation of a mixture with the same microflora. Introducing into each wound a blend of fresh saliva from the oral cavity microflora taken from several mammalian donor provides contamination of the wound more diverse microflora.

Thus, a method is developed for modeling bite wounds, ensuring the accuracy of formation of wounds equal in magnitude, depth and contamination microflora in different groups of experiments on animals, increases the purity of experiments and reliability of the result of the comparative analysis of an experimental study in groups.

Application of the method is possible in experimental veterinary medicine.

The method of modeling the�uchennai wounds, including the application of one injury with the formation of several wounds cover body, characterized in that in experimental animal wounds form tool according to the mechanism of the bite of the teeth of the upper and lower jaws of small mammal and in every wound bring the mixture of fresh saliva from the oral cavity microflora taken from several donor mammal, wherein the saliva take in equal volume from each blood donor and injected a mixture of saliva in the bitten wound in the same dose animals of all groups of experiments.



 

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