Test tray for colloidal properties of human body fluids (versions)

FIELD: medicine.

SUBSTANCE: test tray comprises a case 1 made of an optically transparent material. From one end face, the case 1 has a stop plug 2 with a hole 3, a connecting pipe 4 threaded to connect to either a haemofilter 5, or a cap 7. The case 1 comprises a movable piston 8 connected by a rod 10 to a handle 11. Electrically supplied electrodes 12 are arranged on surfaces of the stop plug 2, piston 8 and on the inner surface of the case 1. The electrodes 12 are connected to contact groups of a device - a laser analyser - through conductors 13, 14. What is disclosed is an alternative version of the structural embodiment of the test tray.

EFFECT: sterile measurement of the colloidal fluid sample.

17 cl, 5 dwg

 

The present invention relates to medicine, in particular to ditches for the study (measurements) Zeta potential and particle size of the dispersed phase of colloidal fluids (blood, lymph etc.) of the human body. To carry out the measurement of the colloidal liquid is first poured into a cuvette containing electrodes to which voltage is supplied to create in a colloidal liquid of an electric field in accordance with the method of dynamic light scattering. Then the cuvette with the sample of the colloidal liquid is placed in the sample compartment of the laser analyzer (laser correlation spectroscopy), which produces the necessary measurements.

A device for sampling blood for research by centrifugation of the sample containing hermetically closed by an elastic stopper with vacuum tube volume, and the elastic tube forms automatically closes the shutter after piercing needle; when the cavity of the tube is provided with a float in the form of an elongated body of uniform cross-section adapted for placement in the mass of red blood cells to stretch the layer of platelets and leukocytes, and means for forming a dome, a stabilizing layer of plasma in centrifuged blood samples [1].

This was from the selection of blood for only �La research taken blood samples by centrifugation and is not intended to measure Zeta potential and particle size of the dispersed phase liquid environments (e.g., blood or lymph) using laser analyzers.

It is also known a device for sampling blood comprising at least a tube consisting of a body and the internal cavity, and the injection needle; wherein it comprises a needle for connection with a container for blood, and the injection needle attached to one end of the housing tube, and a needle for connection with a container for the blood attached to the other end of the casing tube, in addition, the housing tube includes at least two regions which have different values of ultimate tensile strength and/or different values of the maximum forces that can withstand, not collapsing, identical elements of these areas [2].

This device for the collection of blood enables blood sampling directly from the human body, as designed by the design injection needles for General purposes, however, it does not satisfy in General the requirements of sterility and containment, bezkonechnosti the studied fluids with a person and the external environment, the minimality of the fence (the amount should not exceed 2-3 milliliters), the feasibility of the measurement of Zeta potential and the size factor of the particles of the dispersed phase liquid biological media.

The applicant set itself the task of designing the cell, before�oznachennoe for the study of colloidal properties of liquids (blood, lymph and so on) of the human body with the use of the devices of laser correlation spectroscopy, allowing effectively to carry out measurements, in particular the Zeta potential of such liquids, for which the construction of the cell must be adapted for joint operation with a special device, namely a laser analyzer; wherein the cuvette must be sterile, airtight, convenient and safe in operation, have a minimum working volume not exceeding 2-3 ml, and the procedure of filling the cuvette be carried out without contact of the investigated fluids with a person and the external environment. The above positive practical result was achieved by a new combination of essential structural features of the claimed cell for studies of the colloidal properties of the fluid environment of the human body as presented in the following formula of the invention: "cell for studies of liquid colloidal properties of biological fluids of the human body with the use of the devices of laser correlation spectroscopy, comprising a transparent thin-walled cylindrical body having one end with a plug with a hole for passage into the body fluid through made in concert with it the connecting pipe, fitted with internal or external thread for screw �connection with tube or hemofilter, connected through a medical tube with a medical needle, intended for the detention therein of uniform elements of blood or lymph and transmission into the body only in their plasma, piston rigidly connected with the stem ending in a knob, located inside the housing for translational movement along its longitudinal axis, the stroke of which is limited to clamps, and ensuring a tight contact between its lateral cylindrical surface and the inner cylindrical surface of the housing, wherein the cuvette is provided with electrodes placed on the surfaces of the plugs, the piston and the inner surface of the cylindrical body, contact with the investigated colloidal liquid and simultaneously with the inner cylindrical surface of the housing and connected to the contact groups of the device-analyzer via the electrical conductors; a transparent thin-walled cylindrical body made of glass or plastic; the cap and the cylindrical body is made whole at the same time by injection molding or pressing; cover is made separately from the cylindrical body and connected thereto by means of a sealed threaded connection or by gluing, or pressing or welding; the handle of the piston rod is made in the form of a button; the tabs above �ispolneny in the form of projections on the lateral inner surface of the housing, limiting the maximum volume of the investigated colloidal liquid; the electrodes are conductive metal plates of different shapes in plan in the form of circles, or rectangles, or trapezoids, or sectors, or rings; the electrodes are flush (level) with the inner cylindrical surface of the housing, repeating its shape, by pressing or by injection molding; a cylindrical housing through openings that mimic the shape of the electrodes hermetically pressed into these holes; it swept volume does not exceed 2-3 milliliters; cell for studies of liquid colloidal properties of biological fluids of the human body with the use of the devices of laser correlation spectroscopy, consisting of made of optically transparent thin-walled material body, whose cross section is circular or square or rectangle or any other form geometric shapes, with one end having a cap with hole for suction of colloidal fluids inside it through made integral with the connecting pipe stub associated with hemofilter or tube, and the other end of the said casing is also blanking plate with hole and a second connecting tube, Nieuwe� thread for connection by means of a tube adapter with a syringe or made Threadless, filled with a rubber-like substance, which is pierced by a medical syringe needle, wherein the cuvette is provided with electrodes attached to the inner surfaces of the plugs located at both ends of the thin-walled casing completely filled with his biological fluid or on the side surfaces of the thin-walled casing that is in contact with the investigated colloidal liquid and simultaneously with the inner surface of the thin-walled casing and connected to the contact groups of the device-analyzer via the electrical conductors; thin-walled casing is made of glass or plastic; made integral with the plug connecting tube is provided with internal or external thread designed for screw connection with hemofilter or with tube; the electrodes are flush (level) with the inner surface of the thin-walled housing, repeating its shape, by pressing or by injection molding; the thin walled casing through openings that mimic the shape of the electrodes hermetically pressed into these holes; the working volume of the thin-walled casing does not exceed 2-3 milliliters".

The invention is illustrated by drawings, where Fig.1 is a perspective view of the cell for studies of colloidal properties of liquid body man�, made according to the present invention, a variant of the cuvette syringe type: type A) - cuvette Assembly, type B) - cell with detached hemofilters, type b) - the sample compartment of the laser analyzer; Fig.2 is a cylindrical body with a flanged stub pipe for filling the working volume of the test liquid of the cell in Fig.1; Fig.3 - options A), B), b) securing the electrodes on the inner surfaces of end caps and piston that is in contact with the test liquid in the cuvette of Fig.1; Fig.4 - mounting options the electrodes on the inner surface of the cylindrical body of the cuvette of Fig.1; Fig.5 is a perspective view of the cuvette for research colloidal properties of the fluid environment of the human body made according to the present invention, a variant of the cell transmission type (feed-through cuvette: A) cuvette Assembly with tube adapter and syringe; B) cell assembled with a syringe and needle; B) - ditch the transmission type in a disassembled state.

The proposed ditch syringe type (Fig.1) for studies of colloidal biological fluid environment of the human body with devices laser spectroscopy consists of a thin-walled housing 1 of cylindrical shape, made of an optically transparent material such as glass or transparent plastic. One end of the housing 1 and�States the plug 2 with a hole 3 for the passage of fluid into the housing 1 through the connecting pipe 4, made integral with the cap 2. Connecting tube 4 has an internal thread or external thread for a screw connection or by hemofilter 5 through the adapter in the form of a tube 6, either with the tube 7. Hemofilter 5 is designed for the detention therein of uniform elements, such as blood or lymph, and passing into the housing 1 only their plasma.

Cap 2 and a cylindrical body 1 can be performed at the same time by injection molding or extrusion. Cap 2 can also be implemented separately from the cylindrical body 1 and connected with it by means of a sealed threaded connection or by gluing, pressing or welding.

Inside the housing 1 is inserted a piston 8 which can move translationally along the longitudinal axis 9 of the housing 1 providing a sealed contact between the lateral cylindrical surface of the piston 8 and the inner cylindrical surface of the housing 1. The piston 8 is rigidly connected to the rod 10, the end of which has a pen, for example, in the form of a button 11. The stroke of the piston 8 from the cover plate 2 may be limited to clamps, for example, in the form of projections (not shown) on the side of the inner surface of the housing 1, which limit the maximum amount of colloidal biological fluids for research.

In the design of the cuvette is provided by the electrodes 12, which is supplied to �electric voltage to create an electric field by the method of dynamic light scattering to measure Zeta potential and particle size of the dispersed phase of the investigated colloidal biological fluids using special laser analyzer. The electrodes 12 may be arranged on the surfaces of the stub 2 and the piston 8 that is in contact with the test fluid and the inner surface of the cylindrical body 1. The electrodes 12 are electrically conductive metal plates with different shape in plan: in the form of circles, rectangles, or trapezoids, sectors or rings. Possible variants of the electrodes 12 and their mounting on the surfaces of the working volume of the cuvette shown in Fig.3 and 4. The electrodes 12 are connected with the contact group of the device - laser analyzer - through conductors 13, 14.

In case of installation of the electrodes 12 on the inner cylindrical surface of the housing 1 is necessary to ensure the smoothness of this cylindrical surface. For this purpose, the electrodes 12 should be flush (level) with the inner surface of the cylindrical housing 1, repeating its shape. This may be accomplished by molding or by injection molding. It is also possible when in the cylindrical body 1 are made through holes (not shown) that mimic the shape of the electrodes 12; electrodes 12 with tightly pressed into these openings.

Ditch the transmission type (Fig.5) consists of a thin-walled housing 15 whose cross-section may have different shapes (circle, quadras�the one rectangle or any other geometric shape) and the syringe 16 with a piston 17, piston rod 18 with a handle 19. The housing 15 is made of an optically transparent material (glass, plastic). One end of the body 15 has a cap 20 with hole for suction of colloidal biological fluid into the housing 15 through the connecting pipe 21 made integral with the cap 20. Connecting tube 21 has an internal or external thread for a screw connection or by hemofilter 22 or tube 23. The opposite end of the housing 15 also has a cap 24 with a hole and the connection tube 25 made integral with the cover 24. This tube 25 may be threaded for connection with a tube adapter 26 or tube 27, or not to have a thread, but being hermetically filled with a rubbery substance that can pierce the medical needle 28 of the syringe 16.

Manufacturing technology checkpoint of the cell is similar to technology of manufacturing the syringe of the cell.

The electrodes 12 may be in this embodiment, the cuvette is located on the inner surfaces of the plugs 20, 24. In this case, the thin-walled casing 15 should completely be filled with biological fluid. The electrodes 12 may have various shapes in plan and placed on the side surfaces of the cuvette of the same ways ways syringe for the cell.

And�the acquisition is as follows:

Collection procedure colloidal biological fluid using a syringe option of the cell is as follows. The piston 8 through the stem 10 with the handle 11 is pressed to the cap 2. Unscrewed the tube 7 at the end of the connecting tube 4 and instead screwed hemofilter 5 through the adapter in the form of a tube 6 having a reciprocal threaded and made integral with hemofilters 5. With the opposite end of hemofilter 5 is connected through a pipe 29 with a medical needle (not shown) through which the biological fluid to otkazyvatsa from the container, or to get from Vienna or cavities within the human body. Sampling of biological fluids is carried out using a piston 8, which is moved manually from the cover plate 2 at the desired distance. For the study of biological fluids requires about 0.5 to 2 ml of this liquid. Then hemofilter 5 can be unscrewed and the tube 4 is closed by navinchivanie tube 7. After that the tray fits in the sample compartment 30 laser analyzer, where measurements are colloidal properties of liquid biological media.

Collection procedure of the liquid by passing options of the cell is as follows. The syringe 16 is attached to the thin-walled casing 15 of the cell either through a tube adapter 26, or by puncturing a rubber-like substance that fills the connecting pipe 25. Creating a vacuum in the syringe 16 through the stem 18 of the piston 17, the biological fluid is sucked in the thin-walled casing 15 of the cuvette. After filling a predetermined volume of a thin-walled casing 15 of the syringe 16 with transition tube 26 or a medical needle 28 is disconnected and the connecting hole of the tube 25 is closed by a plug 27. Then detaches hemofilter 22, and the hole in the coupling tube 21 is also closed by navinchivanie tube 23. After that, the body 15 with a checkpoint of the cell fits in the sample compartment 30 laser analyzer, where measurements of the colloidal properties of liquid biological environment of the organism.

The inventive cuvette for studies of the colloidal properties of the fluid environment of the human body with the use of the devices of laser correlation spectroscopy, namely, laser analyzers (Zetatrac, Zetasizer Nano, etc.), the principle of which is based on the method of dynamic light scattering (photon correlation spectroscopy), to ensure effective study (measurement of Zeta potential and particle size of the dispersed phase) liquid biological fluids of the human body, such as blood, lymph and other bodily fluids of the same type, sterility, integrity and security of medical research, as well as contactless investigated� fluids with a person and the external environment, the ability to collect liquids not only from containers for the collection of blood and lymph, but also directly from the human body using medical needles.

Sources of information

1. Description of the invention to the patent of Russian Federation №2095024 "Device for sampling blood samples for research when centrifuging this sample, A61B 5/14, stated 01.07.1994 G., published 10.11.1997.

2. Description of the invention to the patent of Russian Federation №2255655 "Device for the taking of blood A61B 5/15, stated 13.02.2004 G., published 10.07.2005.

3 description of the invention to the patent of Russian Federation №2365333 "Trial of the small sampling tube contained in the body fluid, in particular blood, A61B 5/15, A61B 5/153 claimed 21.10.2005 G., published 10.12.2008..

4. Description of the invention to the patent of Russian Federation №2320263 "Device for drawing blood", A61B 5/15, stated 16.12.2002 G., published 27.03.2008

1. Cell for studies of liquid colloidal properties of biological fluids of the human body using laser correlation spectroscopy, comprising a transparent thin-walled cylindrical body having one end with a plug with a hole for passage into the body fluid through made in concert with it the connecting pipe, fitted with internal or external thread for screw connection with tube or hemofilter, connected through a medical tube with medical needles�th, intended for detention therein formed elements of the blood or lymph and transmission into the body only in their plasma, piston rigidly connected with the stem ending in a knob, located inside the housing for translational movement along its longitudinal axis, the stroke of which is limited to clamps, and ensuring a tight contact between its lateral cylindrical surface and the inner cylindrical surface of the housing, wherein the cuvette is provided with electrodes placed on the surfaces of the plugs, the piston and the inner surface of the cylindrical body, contact with the investigated biological fluid and simultaneously with the inner cylindrical surface of the housing and having the opportunity to connect with the contact groups of the device-analyzer via the electrical conductors.

2. Cuvette according to claim 1, in which a transparent thin-walled cylindrical body made of glass or plastic.

3. Cuvette according to claim 1, in which the cap and the cylindrical body is made whole at the same time by injection molding or extrusion.

4. Cuvette according to claim 3, in which the cover is made separately from the cylindrical body and connected thereto by means of a sealed threaded connection, or by gluing, or pressing, or welding.

5. Ditch p� p. 1, in which the handle of the piston rod is made in the form of a button.

6. Cuvette according to claim 1, in which the above-mentioned clamps made in the form of protrusions on the lateral inner surface of the housing, limiting the maximum volume of the investigated biological fluid.

7. Cuvette according to claim 1, in which the electrodes are conductive metal plates of different shapes in plan in the form of circles, or rectangles, or trapezoids, or sectors, or rings.

8. Cuvette according to claim 7, in which the electrodes are flush with the inner cylindrical surface of the housing, repeating its shape, by pressing or by injection molding.

9. Cuvette according to claim 7 or 8, in which a cylindrical housing through openings that mimic the shape of the electrodes hermetically pressed into these holes.

10. Cuvette according to claim 1, in which the working volume does not exceed 2-3 milliliters.

11. Cell for studies of liquid colloidal properties of biological fluids of the human body using laser correlation spectroscopy, consisting of made of optically transparent thin-walled material of the housing, with one end having a cap with hole for suction of body fluids inside it through made integral with the connecting stub pipe, tie�nnow with hemofilter or with tube, and at the other end of the said casing is also blanking plate with hole and a second connecting tube, a threaded connection by means of a tube adapter with a syringe or made Threadless filled with a rubber-like substance, which is pierced by a medical syringe needle, wherein the cuvette is provided with electrodes attached to the inner surfaces of the plugs located at both ends of the thin-walled casing completely filled with his biological fluid or on the side surfaces of the thin-walled casing that is in contact with the investigated biological fluid and simultaneously with internal
the surface of the thin-walled casing and having the opportunity to connect with the contact groups of the device-analyzer via the electrical conductors.

12. Cuvette according to claim 11, in which the cross-section thin-walled housing has the shape of a circle or square or rectangle.

13. Cuvette according to claim 11, in which the thin-walled casing is made of glass or plastic.

14. Cuvette according to claim 11, which is made integral with the plug connecting tube is provided with internal or external thread designed for screw connection with hemofilter or with tube.

15. Cuvette according to claim 11, in which the electrodes are flush with the inner surface of thin-walled building�sa, repeating its shape, by pressing or by injection molding.

16. Cuvette according to claim 11 or 15, in which the thin walled casing through openings that mimic the shape of the electrodes hermetically pressed into these holes.

17. Cuvette according to claim 11, in which the working volume of the thin-walled casing does not exceed 2-3 milliliters.



 

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FIELD: medicine.

SUBSTANCE: psychological tests "10 words" by A. Luria and "personality levels and situational anxiety" by Spilgerber-Khanin are carried out to trained workers. Indices of long-term memory and individual anxiety are determined. Then, a discriminant function F is evaluated. The obtained result is compared with the constant, and if F is larger than the constant, the worker is related to a group with early signs of vinyl chloride intoxication, and if F is lower than the constant - to a group without signs of vinyl chloride impact on the organism. If F is equal to the constant the situation is considered indefinite.

EFFECT: method makes it possible to detect early signs of the toxic impact of vinyl chloride with the application of the most informative diagnostic indices and in this way contributes to the reduction of the volume of paraclinic examinations.

1 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: body weight, physiological requirements and degree of disease severity are determined. That is followed by calculating an infusion therapy extent by formula: V=Ce×BW+PR+300 ml 5% glucose, wherein V is the infusion therapy extent, ml; Ce is an encephalopathy coefficient: 0.02 in prodromal period, 0.03 in manifested psychosis, 0.04 in chronic alcoholic encephalopathy; BW is patient's body weight, gram; PR are the physiological requirements for one day, ml.

EFFECT: method provides the effective infusion therapy in the given category of patients by the accurate determination of the therapy extent caused by taking into account the stages of alcoholic encephalopathy development.

3 ex

FIELD: medicine.

SUBSTANCE: diagnostic test with a 6-minute walk is performed to a patient. Additionally 5 mg of torasemide are given to the patient after the test. Then the test is repeated after one day and if the distance of the 6-minute walk increases by not less than 15 meters, chronic heart failure (CHF) is diagnosed.

EFFECT: method makes it possible to increase the accuracy of differential diagnostics of chronic heart failure in cardiovascular patients with dyspnoea due to the introduction of torasemide.

1 dwg

Blood taking unit // 2547076

FIELD: medicine.

SUBSTANCE: invention refers to medical equipment, namely to a blood taking unit. The unit comprises a cannula sleeve, which limits a chamber, an inlet cannula, an outlet cannula, a closed hose and a ventilation mechanism. The inlet cannula limits an axis, comprises a distal edge and a cavity passing through it. The inlet cannula is attached to the cannula sleeve, so that the distal edge of the inlet cannula is arranged outside the cannula sleeve and the cavity passing through the inlet cannula is connected to the chamber. The outlet cannula comprises a proximal edge and a cavity passing through it. The outlet cannula is attached to the cannula sleeve so that the proximal edge of the outlet cannula is arranged outside the cannula and the cavity of the outlet cannula is connected to the chamber. The closed hose covers the section of the outlet cannula arranged outside the cannula sleeve. The ventilation mechanism generates the connection between the chamber and environment. The ventilation mechanism comprises a tubular insert, which limits a flow passage through it, and a membrane arranged across the fluid passage. The membrane is made of an air-permeable and blood-impermeable material. The tubular insert of the ventilation mechanism penetrates into the tubular flange extending from the cannula sleeve and comprises an outer ring formed on its outer surface. The outer ring adjoins an edge from the side opposite to the cannula sleeve; the outer diameter of the outer ring is equal to the outer diameter of the tubular flange.

EFFECT: using the invention enables providing the safer treatment of patients; the device is simple and inexpensive.

12 cl, 3 dwg

FIELD: physics.

SUBSTANCE: determination is ensured by making the conclusion on psychophysiological conditions of a person by variation in time of the ratio of absolute magnitude of arbitrary jitter of speech signal main tone period, duration of pauses in speech signal, duration of key depression and intervals between key depressions, duration of depression and intervals between depressions of left mouse key, mouse motion signal and image oscillation period exceeding the threshold to their total number.

EFFECT: higher accuracy of determination.

8 cl, 14 dwg

FIELD: medicine.

SUBSTANCE: method involves carrying out ultrasonic scanning examination of subclavian artery over its whole extent in physiological arm position with arterial blood pressure being measured in the middle one third of the arm. Next, when applying compression tests, blood circulation parameters variations are recorded in distal segment of the subclavian artery with arterial blood pressure being concurrently measured. Three degrees of superior thorax aperture syndrome severity are diagnosed depending on reduction of linear blood circulation velocity and arterial blood pressure compared to their initial values. Mild one takes place when linear blood circulation velocity reduction reaches 40% and arterial blood pressure 20% of initial level, moderate one when linear blood circulation velocity reduction reaches 70% and arterial blood pressure 50% and heavy one when linear blood circulation velocity reduction is greater than 70% of initial level and arterial blood pressure is greater than 50% to the extent of no blood circulation manifestation being observed in the subclavian artery.

EFFECT: high accuracy of diagnosis.

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