Method for assessing functional state of haemostasis system
SUBSTANCE: invention refers to medicine, namely to haemocoagulogy, and can be used for detecting a group of individuals suffering a risk of haemocoagulation complications. Substance of the method: measuring initial blood coagulation trace amplitude, determining initial and final blood coagulation parameters and comparing them to corresponding normal blood coagulation parameters; if observing oppositely directed deviations, the disturbed functional state of the haemostasis system is diagnosed. A time constant is determined according to a calibration curve; the calibration procedure a priori covers two measured U1, U2 and known U01, U02 values of lower t1 and upper t2=kt1 limits of the adaptive range; the calibration curve U0i is a blood limit strength function compensating an uncertainty T* of the random time constant T0 and relating the reference Uri and measured Ui characteristics by normalising the measured values by the known ones the calibration curve U0i shows the actual values of the time constant T0 and blood limit strengthU0 which are used to draw the calibration curve of the blood limit strength, reference performance Uri
and to determine initial Ti and final Tf blood coagulation parameters wherein Ui, Uf are the normalised initial and final blood coagulation thresholds.
EFFECT: invention enables reducing a procedure error by tens orders, increasing a coagulation time accuracy by 4 orders, and reducing the efficiency three times as much that eventually provides higher metrological effectiveness of computer analysers applicable for the purpose of automating the process of detecting the individuals having a risk of haemocoagulation complications.
The present invention relates to medicine, namely to hemocoagulase, and can be used to identify individuals at risk of hemocoagulation complications.
Known instrumental method of assessing the functional state of the hemostatic system - thromboelastography (TEG), which consists in a graphical (or photooptical mechanical) registration viscosity characteristics of blood and plasma in the process of coagulation, with subsequent determination of indicators of thromboelastogram characterizing the investigated process [USSR Author's certificate N 1520450, M. CL G01N 33/86, publ. 07.11.89, BI N41].
The disadvantages of this method are the low sensitivity and reproducibility, inability to detect subtle changes in the blood clotting system and to provide an analytic assessment of the identified violations.
A method of determining the functional state of the hemostatic system by registering electrocochleography blood [see proc. Koblov L. F. Methods and devices for the study of hemostasis. - M.: Medicine, 1975, pp. 75-79], which consists in registration of the change of electrical resistance of the blood sample, poured into a cell with two electrodes. The cell oscillates, whereby the blood is alternately closes and interrupts the electrodes. Record the result of the research has the appearance of a number of perio�quarter of pulses with a repetition rate of 0.1 Hz (6 pulses per minute), the envelope which characterizes the process of blood clotting. The amplitude of the pulses corresponds to the resistance of blood being present in the moment between the electrodes of the measuring cell. When assessing electrocochleography take into account the following parameters: T1 is the starting time of coagulation: T2 - the end time of coagulation; T - duration of the drawdown; Am is the maximum amplitude; AO is the value of the minimum amplitude. To change these settings, you get the idea of the different disorders of blood coagulation.
The disadvantages of this method are inertness, relatively low accuracy and sensitivity of measurements due to leakage intensive side of physico-chemical processes related to movement of the electrodes and the test medium relative to each other.
A method of determining the functional state of the hemostatic system [see RF patent №2109297, G01N 33/86, 1998], which consists in the fact that conduct measurements of the amplitudes of the recording process of blood clotting in the beginning, then after one, two and three minutes from the beginning, determine the speed of blood clotting for the second and third minute, calculate return them to the magnitude and compare all four of the same coagulation parameters were normal. In the presence of mixed deviations diagnose violation fu�csinalni the status of the hemostasis system.
The disadvantages of this method are the low accuracy and the duration of its execution.
For the prototype accepted method of determining the functional state of the hemostatic system [see RF patent №2430380, G01N 33/86, 2011], which consists in the fact that conduct the measurement of the amplitude of the recording process of blood clotting in the beginning, define the parameters of beginning and end of the coagulation process electrocochleography blood and compare them with the same indicators of the process of blood coagulation in normal and multi-directional deviations diagnose violations of the functional state of the hemostatic system, record the current amplitude of the resistance of blood in the first time and measuring a second resistance of blood at multiple time from the initial time value, two resistances and the times are marginal blood resistance and time constant, which calculates the resistance of blood at the beginning and end of the coagulation process, and found the arguments specify the indices of the beginning and end of the process of blood coagulation.
The disadvantages of the prototype are relatively low precision and sensitivity of measurement and the duration of the measurement.
Technical challenge of this method are improvement of the metrological efficiency, namely the measurement accuracy, and reduce research time.
The stated technical problem is achieved as follows.
In the method of determining the functional state of the hemostatic system, consisting in the fact that conduct the measurement of the amplitude of the recording process of blood clotting in the beginning, define the parameters of beginning and end of the coagulation process electrocochleography blood and compare them with the same indicators of the process of blood coagulation in normal and multi-directional deviations diagnose violations of the functional state of the hemostatic system, unlike the prototype, determine the time constant for the calibration characteristic, the calibration is carried out a priori for two of the measured and known values of the upper and lower boundaries of the adaptive range of the calibration characteristic is a function of the limiting voltage of the blood that compensate for the uncertainty in the time constant chosen arbitrarily, and connecting the reference and the measured performance at the expense of normalization of the measured values is known, the calibration feature to find the actual value of the time constant and threshold voltage of the blood, which consecutively build the calibration characteristic limit stress of blood, the reference characteristic and determine the indices of the beginning and end of the process of blood coagulation.
Sunstoragetek method explained in Fig.1-4.
1. Determine the time constant T0the calibration function U0i(t).
2. Calibration is done a priori for two well-known reference to Ue(Fig.1 curve 1) and the measured Ui,
3. The calibration characteristic is the characteristic U0i(Fig.1, curve 3) limit stress of blood that compensate for the uncertainty of the time constant T* is arbitrary, and connecting the reference to Ueand measured Uidependencies due to the normalization of the measured values is known (Fig.1, curve 3)
The calibration characteristic U0irestore the characteristic Uiidentical reference
which is as close to the reference curve Ue:
Reference characteristic Ue=U and characteristics, it is identical to, Uiobtained from exponential dynamic performance with desired informative parameters T0U0:
where T0- time constant of the process of hemostasis and U0- limit stress of the blood. The physical meaning of informative parameters follows from the limit ratios:
In practice, one of the informative parameters of the studied characteristics, as a rule, unknown. In this case, one parameter is specified arbitrarily T*, and the second takes the form of a function U0ithat compensates for the ignorance of the first informative parameter. With the help of this funk�AI is calibrated to the measured curve.
Asked by arbitrarily setting T*=const is the unknown actual value of the time constant T0. To compensate for the arbitrariness of the constant T* marginal blood stress U0to turn into the characteristic U0icompensating for the lack of knowledge of the time constant T0.
Calibration function for known parameters T0U0is exponential dynamic response (2).
The calibration characteristic U0iExpress from the system of equations with known parameters T0U0characteristics of Uewhich reference (obtained by fitting the experimental data), and characteristics of Uibeing measured, with an arbitrary constant T* and the characteristic U0i:
Divide one equation of the system to another to Express the calibration feature:
In accordance with the laws of the calibration and te=tishould the calibration characteristic U0irelating the reference and the measured curves:
Therefore, the calibration characteristic is f�NCCI limit stress blood compensating the uncertainty in the time constant selected at random (Fig.1, curve 3).
4. The calibration characteristic U0ifind the actual value of the time constant T0and limit stress blood U0that are informative parameters that delivers the optimum calibration characteristic. From specifications (4) form a system of equations for
Dividing one equation of the system (5) to another and prologue�mirowave, define algorithm time constant T0:
Expressing T0from the first and second equations of system (5) and equating them to each other
find an algorithm to determine the maximum operating voltage of the blood:
5. On the actual values of the time constant T0and limit stress blood U0consistently build the calibration characteristic U0ilimit stress of the blood and the reference characteristic Ue. The result of calibration is the identity of the measured characteristics of Uireference to Uei.e. Ui≡Ue.
For informative parameters (6) and (7) build (approximate) calibration characteristic U0i(4) (Fig.1 curve 3), which is found according to (3) the calibrated characteristic Udi(Fig.1 curve 4), identical with a desired reference feature.
Found informative parameters define the start and end of the clotting process:
The values obtained in the beginning of Tnand the end of TKthe clotting is compared in magnitude with the same parameters of clotting healthy people. Upon detection of mixed abnormalities diagnose the violation of the functional state of the hemostatic system.
1. Prove metrological effectiveness of the proposed method relative to the prototype of a methodical error of ε1(Fig.2):
From the graph (Fig.2) shows that the truncation error of the prototype varies from 3% to 12%.
Rate truncation error ε2between reference 1 and calibrated 4 characteristics (Fig.1)
From the graph (Fig.3) shows that the relative error does not exceed 2.4*10-16or 2.4*10-14% through the use of calibration features in the adaptive range of the normalized values at the borders.
2. Rate metrological efficiency on clotting time.
The start time of coagulation to the reference characteristic (Fig.4, curve 1) TN1=170, end time coagulation TK1=460 for normalized amplitudes Un=7,34 UK=4,33. Found by the algorithms (7) and (8) limiting parameters U0=10, T0=550.
Find valid time values (Fig.4, curve 4) for the algorithms (8):
Calculate the error start time coagulation characteristics between 4 and 1
and the end of the coagulation
In characteristic 2 (Fig.4, curve 2) normalized amplitude thresholds Un=7,34, UK=4,33 find clotting time of the prototype Th 2=130 and TK2=375.
Estimate the uncertainty end time coagulation 1 between the reference and measured 2 characteristics
and error start coagulation
The efficiency η on the accuracy of clotting time was calculated as the ratio of the first to the second error
Thus, the determination of the actual values due to the normalization of the measured values famous�mi on the calibration characteristic limit stress blood unlike known solutions, reduces truncation error by tens of orders of magnitude, the accuracy of the clotting time increases to about 4, and the speed reduces three times, which ultimately improves measurement efficiency of the computer analyzers to automate the identification of persons at risk of hemocoagulation complications.
Method for determining the functional state of the hemostatic system, consisting in the fact that conduct the measurement of the amplitude of the recording process of blood clotting in the beginning, define the parameters of beginning and end of the coagulation process electrocochleography blood and compare them with the same indicators of the process of blood coagulation in normal and multi-directional deviations diagnose violations of the functional state of the hemostatic system, characterized in that the determined time constant for the calibration characteristic, the calibration is carried out a priori for two measured U1U2and known U01U02values of the lower t1top and t2=kt1the boundaries of the adaptive range of the calibration characteristic U0iserves the function of limiting the voltage of the blood that compensate for the uncertainty of the time constant T0chosen arbitrarily T*, and connecting the reference to Ueand measured Uicharacteristics W� by normalization of the measured values is known
the calibration characteristic U0ifind the actual value of the time constant T0and the limit voltage U0blood
which consistently build the calibration characteristic limit stress of blood, the reference characteristic Ue
and define the parameters beginning with Tnand the end of TKthe process of blood clotting
where UnUK- normalized threshold voltage of the beginning and end of the process of blood clotting.
SUBSTANCE: invention relates to method of determining platelet resistance to acetylsalicylic acid (ASA) by impedance analysis of aggregation function of platelets in vivo, in which aggregation activity is analyses after incubation of biological material sample with ASA with application of aggregation inducer, and aggregation of platelets is induced by collagen in optimal concentration 2 mg/ml, and simultaneously with measurement of impedance determination of dynamics of platelet granules release is performed by luminescent method, where before carrying out aggregation samples are calibrated by means of adenosine triphosphate (ATP) standard, value of aggregation amplitude are determined in Ohms by obtained aggregatograms and obtained values are given points: values ≤6 correspond to 0 points, values 7-9 correspond to 1 point, values 10-12 correspond to 2 points, values >12 correspond to 3 points; after that, intensity of ATP release from platelet granules is determined in nmoles and obtained values are given points: values <0.5 correspond to 0 points, values 0.5-1.0 correspond to 1 point, values 1.0-1.5 correspond to 2 points, values 1.5 correspond to 3 points, and then resistance index (RI) is calculated by formula, value of IR parameter points to presence of aspirin-resistance of platelets.
EFFECT: carrying out fast complex diagnostics of platelet resistance to ASA with estimation of functional state of platelet granules before beginning treatment and possibility of preventing development of undesirable thrombotic or hemorrhagic complications.
2 dwg, 1 tbl, 2 ex
SUBSTANCE: invention represents a diagnostic technique for the disturbed thrombocyte aggregation accompanying mucoviscidosis in children involving a thrombocyte aggregation test using the Multiplate aggregometer inducers. Trays with a magnetic mixer and electrodes are added with NaCl 400 mcl at 37°C and immediately added with whole blood 400 mcl from a hirudin test tube, incubated in the chamber for two minutes; the tray is added with 30 mcl of an aggregation inducer specified in a group: soluble thrombin receptor - peptid-6, adenosine diphosphate, arachidonic acid. The thrombocyte aggregation rate is displayed on the screen in the form of a curve, and the sub-curve area U is automatically calculated; the sub-curve area U shows the thrombocyte aggregation state as compared to reference values in the group of healthy children; if the threshold area U has appeared to exceed the reference, the thrombocyte hyperaggregation, while the threshold area U being less than the reference, the thrombocyte hypoaggregation is stated.
EFFECT: invention provides the timely diagnosis of microcirculatory disorders accompanying mucoviscidosis.
2 ex, 1 dwg
SUBSTANCE: invention refers to medicine, particularly to clinical biochemistry, and aims at determining oxidative protein modification in a substance pool of an average molecular weight in a biological medium accompanying any pathological conditions by biochemical examination. The biological medium specified in the blood plasma, erythrocyte or urine is sampled; proteins are deposited by adding 10% trichloroacetic acid; if observing sedimentation, a centrifugation cycle follows at 1000 rpm for 15 minutes; thereafter, 2,4-dinitrophenylhydrazine 0.05 M in hydrochloric acid 2 M is added; the sample is centrifuged at 1000 rpm for 20 minutes; and if observing sedimentation, the sediment is washed twice in ethanol-ethylacetate (1:1), dried on a water bath for 10 minutes and dissolved in urea 8 M; the sample is kept in a boiling water bath for 10 minutes until dissolved completely; the prepared solution is analysed by spectrophotometry. The method is applicable both for single use, and for monitoring of the postoperative oxidation protein modification and average molecule levels.
EFFECT: method provides higher information value of biochemical tests, reducing consumption of the biological material.
9 tbl, 2 ex
SUBSTANCE: invention includes determination of content of soluble fibrin and D-dimers, formed in the process of fibrinolysis, activated in blood sample. In method, in accordance with the claimed invention, level of D-dimers, corresponding to destruction of soluble fibrin and level of D-dimers in sample with border values of the norm, are compared.
EFFECT: test in accordance with the claimed invention can be applied for determining whether resistance to blood coagulation in patient is sufficient.
4 tbl, 3 ex, 2 dwg
SUBSTANCE: method for thrombin activity test in an initially non-reacted mixture of thrombin and fibrinogen (versions) involving the stages: (a) reversible thrombin inhibition by adding an inhibitory solution having pH varying within the range of 8.5 to 11.5; (b) addition of the known amount of fibrinogen to the mixture (or the known amount of a chromogenic or fluorogenic thrombin substrate), (c) reversible thrombin activation by pH reduction to approximately 6.0 to less than 8.5, (d) enabling thrombin reacting with fibrinogen, (e) thrombin activity test initially found in the dry mixture. The method for fibrinogen functionality test in an initially non-reacted mixture of thrombin and fibrinogen (versions) involving the stages: (a) reversible thrombin inhibition by adding an inhibitory solution having pH varying within the range of 8.5 to 11.5; (b) addition of the known amount of thrombin to the mixture (or a thrombin-like enzyme), (c) reversible thrombin activation by pH reduction to approximately 6.0 to less than 8.5, (d) enabling thrombin reacting with fibrinogen, (e) fibrinogen functionality test initially found in the dry mixture.
EFFECT: group of inventions enables higher accuracy of thrombin and fibrinogen activity test.
32 cl, 1 dwg
SUBSTANCE: analyser has a revolving cuvette with a sample in which there is a control ferromagnetic ball, a magnet which can interact with said ball, a coagulation sensor which transmits signals from the ball and having a Hall sensor and a magnet, a signal processing device in form of a power supply unit, a Hall sensor, a microprocessor and a display device included in a common measuring circuit. The analyser is multi-channelled by fitting at least one additional revolving cuvette to form several channels. All cuvettes lie in a temperature-controlled unit included in the common measuring circuit. The longitudinal axis of each cuvette is inclined at an acute angle to the vertical. In the coagulation sensor, the magnet lies opposite the Hall sensor on the opposite side of the cuvette. The magnet is in form of a flat cylinder mounted with possibility of displacement along the cuvette. The analyser is also fitted with a unit for controlling rotation of the cuvettes and, included in the common measuring a circuit, a measurement parameter adjustment unit having rewritable read-only memory, and a timer configured to automatically switch on when a reactant is fed into the cuvette.
EFFECT: use of the invention increases reliability and broadens functional capabilities of the analyser owing to use of a multichannel measuring circuit, and simplifies the measuring procedure by automating the process.
4 cl, 2 dwg
SUBSTANCE: blood plasma is examined in 4 minutes after the beginning of spontaneous red blood cell aggregation for free red blood cell count and cell count in aggregates. A percentage of non-aggregated red blood cells (PNA RBC) by formula PNA RBC=FRBSC×100/(TRBCA+FRBSC) wherein FRBSC is the free red blood cell count, TRBCA are total red blood cells in aggregates. If the PNA RBC is 56 to 30%, I degree of severity is stated, 30% to 4% - II degree of severity, less than 4% - III degree of severity.
EFFECT: use of the invention enables objectifying and increasing precision of evaluation of red blood cell aggregation, evaluating an intensity of patient's microcirculation disorders in a relatively short time, and thereby ensuring well-timed adequate complex of therapeutic measures or corrected therapy.
SUBSTANCE: quantity of fibrin-monomers, dissolved in 0.5 N sodium hydroxide, is determined spectrophotometrically with application of ethanol test. Claimed method of quantitative determination of fibrin-monomers in blood makes it possible to reveal pathological process in organism with 95% reliability.
EFFECT: increase of determination accuracy.
2 tbl, 4 ex
SUBSTANCE: thrombosis monitor comprises: a thrombosis chamber, at least in a part of which there is a thrombogenic material; an inlet tube connected to the thrombosis chamber through which blood flows into the thrombosis chamber; a blood supply container connected to the inlet tube; a feed pump for the container; a pressure sensor for measuring pressure applied to the container. A method of thrombosis monitoring consists in the fact that after introduction of an anticoagulant, blood is supplied from the container to the thrombosis chamber by pressing on a fluid placed on a blood layer and having density less than that of the blood layer; it is combined with anticoagulation blood processing or blood coagulation stimulation, and measurement of pressure applied to the container; the thrombogenic material is placed at least in a part of the thrombosis chamber.
EFFECT: group of inventions provides overall assessment of blood coagulation and platelet-cell thrombosis in a medium equivalent to blood flow for evaluation of efficacy of an antithrombotic drug.
11 cl, 15 dwg, 23 ex
SUBSTANCE: for determination of functional state of hemostasis system record of blood coagulation process is performed, current amplitude of blood resistance in first time moment is registered and second resistance of blood at multiple time moment from initial time value is measured. Two resistances and time moments are used to determine maximum blood resistance and time constant, by which blood resistance at the beginning and end of coagulation process is calculated. Obtained parameters are used to determine indices of beginning and end of blood coagulation process. Obtained indices are compared with of the same name indices of blood coagulation process in norm and in case of differently directed deviations disturbances of functional state of hemostasis system are diagnosed.
EFFECT: invention makes it possible to increase measurement accuracy and reduce examination time.
1 tbl, 4 dwg
FIELD: medicine, laboratory diagnostics.
SUBSTANCE: the suggested studying should be carried out on the glass simultaneously with several inductors by applying minimal inter-taking antilogarithms concentrations of aggregation inductors which correspond at double combination of inductors: ADP 5.0 x 10-8 M, adrenaline 3.0 x 10-9, collagen - dissolving the main suspension 1:8, thrombin 0.075 U/ml; at triple combination of inductors: ADP 10-9 M, adrenaline 10-9, collagen - dissolving the main suspension 1:9, thrombin 0.060 U/ml. The development of aggregation means thrombocytic activation in patients with arterial hypertension at metabolic syndrome. The method enables to evaluate the changes of thrombocytic functional state with combination of inductors more probably present in area of vascular lesion by applying minimal necessary concentrations that develops real conditions at hemostatic initiation in human vessels.
EFFECT: higher efficiency of studying.
3 dwg, 3 ex, 2 tbl
SUBSTANCE: method involves checking consciousness, blood coagulation state, peripheral blood leukocytes number, K+ ions, bilirubin, fibrinogen, hemolysis and hemoglobinuria availability, prothrombin index and exotoxic shock development. Each value is calculated in points as follows. Lucidity is evaluated as -2 points; depression - +3 points; coma - +6 points; lack of changes in blood coagulation system - -2 points; coagulation availability without clinical injuries - +2 points; coagulopathy with clinical manifestation signs - +19 points; K+ ions concentration being less than 3.0 mmole/l - +3 points, from 3.1 to 3.5 mmole/l - -5 points, from 3.6 to 5.0 mmole/l - 0 points, greater than 5.0 points - +7 points, failure in determining K+ ions concentration - 0 points; hemolysis availability - +6 points, its lack - -3 points; hemoglobinuria availability - +8 points, its lack - -1 points; leukocytes number being less than 12.0x109/l - -2 points, from 12,1 to 18.0x109/l - 0 points, higher than 18.0x109/l - +8 points; hourly urine output being less than 30 ml/h - +6 points, greater than 30 ml/h - -2 points; bilirubin content being less than 31 mcmole/l - -2 points, from 30.1 to 50.0 mcmole/l - 0 points, greater than 50.0 mcmole/l - +2 points, failure in determining bilirubin content due to hemolysis being available -+6 points; prothrombin index being equal to or less than 60% - +3 points, greater than 60% - 0 points, failure in determining prothrombin index due to hemolysis being available - +12 points; fibrinogen concentration in blood plasma being less than 2.1 g/l - +4 points, from 2.1 to 4.0 g/l - -1 point, from 4.1 to 6.0 g/l - +1 point, failure in determining fibrinogen concentration due to erythrocyte hemolysis being available - +13 points; exotoxic shock development - +9 points, its lack - -1 point. The points are summed up. The value being greater than +13, admission for treatment in resuscitation department is indicated. The value being less than -13, admission for treatment in therapeutics department is indicated. The value being from -13 to +13, resuscitation expert consultation is advised.
EFFECT: high evaluation accuracy.
FIELD: medicine, laboratory diagnostics.
SUBSTANCE: one should evaluate the time for clotting of plasma under testing in phospholipid-dependent test, moreover, one should apply high- and low-sensitive thromboplastin reagents to lupus anticoagulant to calculate the ratio of indices of prothrombin time prolongation and at its value being either equal to or above 1.1 one should diagnose APS.
EFFECT: shortened terms of research.
1 ex, 4 tbl
SUBSTANCE: method involves analyzing symptoms manifesting initial disseminated intravascular blood coagulation syndrome danger like burn area, availability of upper air passages burn, shock with its severity degree taken into consideration, sepsis development; clinical manifestations of disseminated intravascular blood coagulation syndrome like lung, kidney, liver function insufficiency, cerebral dysfunction, local and multiple hemorrhages, thrombosis, infarction; homeostasis system laboratory analysis data, hyper- and hypocoagulation based on chronometry test data, number of blood platelets, fibrin-monomer complexes, D-dimers, activity of antithrombin III, C and S proteins, XIIa-dependent fibrinolysis plasminogen content, availability of injured erythrocytes, combinations of laboratory tests for recognizing disseminated intravascular blood coagulation syndrome. Each sign under consideration receives a number of points corresponding to its diagnostic significance and integral value is calculated DIBCSIV=(X1+X2+…+Xn)/n, where n is the number of signs taken into consideration. DIBCSIV value equal to 1.0-1.5 units shows physiological norm. The value being between 1.6 and 2.5 units, light disseminated intravascular blood coagulation syndrome is diagnosed. The value being between 2.6 and 3.5 units, disseminated intravascular blood coagulation syndrome of medium severity is diagnosed; 3.6-4.5 points to one heavy severity degree; 4.6 and greater indicates highly severe case of disseminated intravascular blood coagulation syndrome.
EFFECT: high accuracy and objectiveness in differentiating syndrome severity degrees.
FIELD: medicine, diagnostics.
SUBSTANCE: one should study blood components to detect anticoagulant-fibrinolytic activity. Moreover, patient's blood should be sampled: in whole blood one should detect the presence of affected erythrocytes and evaluate the quantity of thrombocytes, in plasma it is necessary to study the activity of antithrombin III, XIIa-dependent fibrinolysis, the content of soluble fibrin-monomeric complexes, in blood serum of the sample taken one should detect the concentration of urea, creatinine, sodium, albumin, total cholesterol and the activity of aspartate aminotransferase, moreover, one should calculate integral value of renal-hepatic deficiency, to put corresponding point for the degree of parameters under testing, then one should calculate integral value of disseminated intravascular clotting (IVDIC) and at its value being 6.3 U and more DIC-syndrome should be diagnosed, moreover, at IVDIC value ranged 6.3-10.1 U it is possible to diagnose latent DIC-syndrome, at 10.2-14.6 - subacute DIC-syndrome and at 14.7 and higher - acute DIC-syndrome should be concluded.
EFFECT: higher accuracy and efficiency of diagnostics.
4 ex, 2 tbl
FIELD: medicine, obstetrics.
SUBSTANCE: the present innovation deals with predicting disadaptive processes in women in dynamics of menstrual cycle. During menstrual cycle beginning since the 1st d to the 21st d one should detect the dynamics for alteration in coefficient of activity of syntoxic adaptation programs (CASAP), calculated by the following formula:
where CST - concentration of blood serotonin, AAT-III - activity of antithrombin III, Aaoa - total antioxidizing activity of plasma, CCD8 + - concentration of T-suppressors, Cad - concentration of blood adrenalin, Cα2MG - concentration of α2-macroglobulin, CMDA - concentration of malonic dialdehyde, CCD4 + - concentration of T-helpers. Moreover, normally CASAP value alters two-fold against the first day of the cycle - since 0.70 up to 1.40 on the 21st d of the cycle, at no alterations in CASAP value one should diagnose female disadaptive alterations leading to failed pregnancy. The innovation enables to perform diagnostics of disadaptive processes in women in dynamics of menstrual cycle followed by prognostic conclusion upon future pregnancy.
EFFECT: higher accuracy of diagnostics.
SUBSTANCE: method involves determining spontaneous blood platelets aggregation and one induced by adrenalin and collagen, thrombocytospecific peptides activity of β-thromboglobulin and thrombocytic factor 4 in blood plasma.
EFFECT: high accuracy of diagnosis.
SUBSTANCE: method involves determining coagulating blood viscosity values like reaction period r, thrombin constant K, maximum amplitude MA, time T for forming fibrin-thrombocytic blood clot, spontaneous blood platelets aggregation intensity Ar, retraction and spontaneous clot lysis total FA. The r being within 5-7 min, Ar from -2 to -6 relative units, K being within 4-6 min, MA within 500-700 relative units, T within 40-60 min and FA equal to 10-20%, low inflammatory process activity is considered to be the case. The r being less than 5 min, Ar equal to -8 to -12 relative units, T less than 40 min and FA less than 10% with no changes in K and MA being observed, inflammatory process activity in chronic glomerulonephritis case is considered to be of high severity degree.
EFFECT: high accuracy of diagnosis; enhanced effectiveness of treatment method selection.
FIELD: medicine, clinical neurology, neurosurgery.
SUBSTANCE: one should study both activation and aggregation of thrombocytes in blood of carotid artery, at the quantity of thrombocytic active forms being above 70% and the number of aggregated thrombocytes being above 9.0% one should predict the development of cerebral ischemic lesion along with stable focal neurological symptomatology, and at the quantity of thrombocytic active forms being below 30% and the number of aggregated thrombocytes being below 8.0% it is possible to predict positive dynamics in the course of the disease mentioned without developing cerebral ischemic lesion.
EFFECT: higher accuracy of prediction.
FIELD: medicine, clinical neurology, neurosurgery.
SUBSTANCE: one should study the level of von Willebrand's factor in patient's carotid artery blood. At its content being below 105% one should predict the development of repeated AICH. The innovation improved information value of testing due to possibility to obtain reliable prediction in latent period, as well.
EFFECT: higher accuracy of prediction.
2 ex, 1 tbl