Diagnostic technique for blood serum glucose, total protein and electrolytes by multi-frequency impedance analysis
SUBSTANCE: method provides blood serum analysis by a bipolar method for multi-frequency impedancemetry to determine impedance modulus (|Z|) and phase angle (φ) at AC low-intensity frequencies 20, 98, 1000, 5000, 10,000, and 20,000 Hz by means of hardware-software complex provided with BIA-lab Compositum software; measurements are taken in a microchamber of 50 mcl; the software automatically calculate the blood serum concentrations of total protein, glucose, chlorides and bivalent ions on the ground of solution of assembled mathematical equations; the result is displayed and can be printed.
EFFECT: higher diagnostic effectiveness by eliminating the need for using chemical agents, reducing the time for analysis, cutting the cost and extending the indications for using the technique.
The invention relates to medicine, namely to laboratory diagnostics, and is intended to improve the efficiency of the study of glucose and total protein in serum.
The closest analogue is a method of biochemical analysis based on photometric measurement of the intensity of the color of the chemical reagent solution of phenolphthalein in determining the concentration of glucose and protein complexes with copper sulfate in the determination of the concentration of total protein (Laboratory techniques: a Handbook / edited by Menshikov V. V. - M.: Medicine, 1987. - S. 175, 232-233, 261-265). Method of biochemical analysis is performed as follows. After protein precipitation with trichloroacetic acid pH protein-free solution with sodium phosphate adjusted to a point close to the optimal action of glucose oxidase, and carry out the enzymatic reaction. The formed hydrogen peroxide in the presence of copper ions oxidize phenolphthalein to phenolphthalein, which in the neutral colorless area, and in the alkaline region is colored in red. The result is recorded by a spectrophotometric method. To determine the total protein concentration to 0.1 ml of serum was added to 5.0 ml of the working solution biuret reagent (4.5 g sagatavoj salt dissolved in 40 ml of 0.2 mol/l sodium hydroxide solution, is added 1.5 g of copper sulfate and 0.5 g of potassium iodide and races�varaut; made up to 100 ml of 0.2 mol/l sodium hydroxide solution; 20 ml of biuret reagent was mixed with 80 ml of 0.5% solution of potassium iodide) and mixed, avoiding formation of foam. After 30-60 minutes to complete the measurement of the optical density of the solution in the photometer in a cell with a layer thickness of 1 cm at a wavelength of 500-560 nm against blank sample. Calculating the concentration of total protein lead via a calibration curve. The concentration of sodium and potassium define a unified method of flame photometry. For this purpose, diluted with water, the serum is sprayed in the form of tiny droplets with a stream of air enters the flame of a gas burner that potassium gives a weak red-violet and sodium bright yellow staining. The color intensity is measured by a photocell. To calculate the concentrations of potassium and sodium used calibration solution. The results calculated by a calibration curve. The concentration of calcium ions is determined using a unified method of colour reaction with cresolphthalein. This reagent reacts with calcium in alkaline medium complex red-violet color, the intensity of which is proportional to the calcium concentration. The reaction mixture was added 8-okshihinolin that binds metals, preventing the determination of the concentration of calcium.
The disadvantages of the prototype: the existing method of biochemical �analysis is not effective enough, requires the use of chemical reagents and laboratory equipment, specially trained medical personnel (a technician) that has a duration of at least 1 hour, is suitable for use in medical institutions and insufficiently cost-effective.
The task of the invention is to simplify the process and expansion of indications for the use of the method.
The problem is solved with the features specified in the claims, such as a method of diagnosis of glucose concentration, total protein and electrolytes in serum, wherein the human serum used bipolar method policestate elektroindustrie with the definition of modular values of impedance (|Z|) and phase angle (φ) at frequencies 20, 98, 1000, 5000, 10000, and 20000 Hz alternating electric current of low power by using a hardware-software complex, equipped with the computer program "BIA-lab Compositum", carry out the measurement in the microcamera volume of 50 μl the program automatically calculates the concentration of total protein, glucose, chloride and divalent ions in the serum based on the solution of a system of mathematical equations, and the result is displayed and can be printed on the printer.
The technical result of the above precisionautofinance essential features - improving the effectiveness of the method by eliminating the need to use chemicals, reducing the run-time studies, cost reduction and the expansion of indications for the use of the method.
A solution to this problem is achieved by measuring the electrical impedance of the blood serum, placed in a special microcamera, bipolar method using policestate sensing an alternating electric current of low power. The computer program "BIA-lab Compositum" (Certificate of state registration of computer programs "BIA-lab Compositum" No. 2012613493, April 13, 2012 copyright Holders: Mishlanov Y. V., Mishlanov V. Yu., Mishlanova I. V., Mishlanova S. L. Authors: Mishlanov Y. V., Mishlanov V. Yu., Mishlanova I. V., Mishlanova S. L.) allows you to register two physical characteristics of bioelectrical impedance: a modular value of the impedance (|Z|) and phase angle (φ) in series at 6 frequencies: 20, 98, 1000, 5000, 10000 and 20000 Hz. At each frequency of the probing AC electric current is recorded 576000 measurements of the analyzed parameters, and a computer program calculates the average value of these indicators is based on the equations of conformity. The entire measurement period is 18 seconds.
The method is as follows. Blood studies are taken from �octavos a vein or a finger of the patient. Isolated serum and 50 µl her make the micro camera unit using the pipette. The electrodes of hardware and software complex, for example, "BIA-lab Spiro" (Patent RF №2487662, publ. 20.07.13. IPC A61B 5/08) or other (Patent RF "Device for measuring impedance of biological media" No. 2462185 from 19.07.2011. IPC A61B 5/08, publ. 27.09.12) connected to the contacts of the micro cameras include the instrument in a second-by-second visualization of the results at a frequency of 20000 Hz, then write the results of the study successively at frequencies of 20, 98, 1000, 5000, 10000 and 20000 Hz. Total recording time of 18 seconds (the program automatically changes the modes of registration of bioelectrical impedance 6 frequency bands, recording the results of measurements for 3 seconds at each frequency of the probing AC electric current, which corresponds 576000 measurements of parameters measured). The result is recorded automatically in a folder named after the name of the patient. Total execution time of the study less than 1 minute.
Diagnosis the concentration of total protein, glucose, potassium ions and calcium in the sample of blood serum is carried out in automatic mode with the help of the computer program "BIA-lab Compositum", and calculate the concentration according to formulas that take into account the magnitude of the modular value of the impedance |Z| and f�basic angle φ, obtained by probing serum alternating electric current of low power on 6 frequencies: 20, 98, 1000, 10000 and 20000 Hz.
Used the following equation corresponding to the used measuring chamber. To calculate the concentration of glucose: SGL=1,86*Exp(-0,058*f98), where SGL is the concentration of glucose in mmol/l, f98 - value of the angle φ on the frequency of the probing AC electric current 98 Hz. Calculating the concentration of total protein was carried out using the formula: Sa=171,8529*Exp(-0,85*(f10000/f20000)), where Sa is the total protein concentration in g/l, f10000 - value of the angle φ on the frequency of the probing AC electric current 10000 Hz, f20000 - value of the angle φ on the frequency of the probing AC electric current 20000 Hz. Calculating the concentration of calcium ions was carried out using the formula: CCA=-2,7667+1,4374*Log10(Z5000), where Sa is the total protein concentration in mmol/l, Z5000 - the value of the modular values of |Z| at the frequency of the probing AC electric current of 5000 Hz. Calculating the concentration of potassium ions was carried out using the formula: SC=3,0793-0,009*(Z1000/f1000)-0,00001274*(Z1000/f1000)∧2, where ck is the concentration of potassium ions in mmol/l, Z1000 - the value of |Z| at the frequency of the probing AC electric current of 1,000 Hz, f1000 - value of the angle φ on the frequency of the probing AC electric current of 1,000 Hz.
Error method to determine the concentration of glucose does not turn into a�exceeds 0.5 mmol/l, to determine the total protein concentration is not more than 10 g/l, to determine the concentration of potassium ions is not more than 0.5 mmol/l, calcium - not more than 0.3 mmol/L.
Examples of specific performance.
Patient B., 51 years old, diagnosed with multiple myeloma, diffuse alopecia form, variant G.
The total protein concentration in serum, a certain method is 93,1 g/l. the Concentration of glucose in serum, determined by, is 6.0 mmol/l. the Concentration of potassium ions is 4.5 mmol/l. the Concentration of calcium ions is 2,36 mmol/L.
Method policestate electrical impedance analysis using a hardware-software complex, equipped with the computer program "BIA-lab Compositum", defined concentration: protein and 86.5 mmol/l, glucose 5.6 mmol/l, potassium of 4.7 mmol/l, sulphate of 2.3 mmol/L.
The presented example demonstrates the high accuracy of the application policestate electrical impedance analysis for the diagnosis of the concentration of total protein, glucose, potassium ions and calcium in blood serum in clinical practice.
Patient K., 60 years. Diagnosis: diabetes mellitus, type 2, average flow, uncompensated. Complications: Micro - and macroangiopathy, lipodystrophy liver, diabetic foot syndrome. KHAN 1 tbsp.
The concentration of glucose in serum, ODA�divided method is 12.8 mmol/L. Method policestate electrical impedance analysis using a hardware-software complex, equipped with the computer program "BIA-lab Compositum", defined glucose concentration of 13.0 mmol/L.
The presented example demonstrates the high accuracy of the application policestate electrical impedance analysis for the diagnosis of glucose concentration in the serum of the patient with diabetes mellitus in a state of decompensation of carbohydrate metabolism and excess of the normal range of glucose concentration in the blood.
Patient K., aged 29. Diagnosis: diabetes mellitus, type 2, average flow, uncompensated. Complications: Micro - and macroangiopathy, lipodystrophy liver, retinopathy 1 article Hypoglycemic condition on the background of alcoholic intoxication mild. Competing diagnosis: Pneumonia, focal, in the lower lobes of the right and left, not severe. ONE 1 tbsp.
The concentration of glucose in serum, determined by, is 2.3 mmol/L. Method policestate electrical impedance analysis using a hardware-software complex, equipped with the computer program "BIA-lab Compositum", determined the concentration of glucose was 2.7 mmol/L.
The presented example demonstrates the high accuracy of the application policestate electrical impedance analysis in SL�tea diagnosis of glucose concentration in the serum of the patient with diabetes mellitus in a state of decompensation of carbohydrate metabolism and a decrease in the concentration of glucose in the blood.
The proposed method is characterized by the following features. In the proposed method adopts the definition of electrical impedance of the blood serum in a special microcamera volume of 50 µl in the mode policestate analysis that allows to determine the concentration of total protein, glucose and electrolytes on the basis of mathematical solutions of the system of equations based on the experimental results.
Method for the diagnosis of glucose concentration, total protein, potassium ions and calcium in blood serum, characterized in that for the human serum used bipolar method policestate elektroindustrie with the definition of modular values of impedance (|Z|) and phase angle (φ) at frequencies 98, 1000, 5000, 10000, and 20000 Hz alternating electric current of low power by using a hardware-software complex, equipped with the computer program "BIA-lab Compositum", carry out the measurement in the microcamera volume of 50 µl, calculate the concentration of total protein, glucose, ions of potassium and calcium in blood serum, based on the solution of a system of mathematical equations, wherein the glucose concentration is calculated by the formula: SGL = =l,86*Exp(-0,058*f98), where SGL is the concentration of glucose in mmol/l, f98 - value of the angle φ on the frequency of the probing AC electric current 98 Hz, the calculation �concentratii total protein is carried out using the formula: Sa=171,8529*Exp(-0,85*(f10000/f20000)), where Sa is the total protein concentration in g/l, f10000 - value of the angle φ on the frequency of the probing AC electric current 10000 Hz, f20000 - value of the angle φ on the frequency of the probing AC electric current 20000 Hz, calculate the concentration of calcium ions is carried out using the formula: CCA = =-2,7667+1,4374*Log10(Z5000) where CCA is the concentration of calcium ions in mmol/l, Z5000 - the value of the modular values of |Z| at the frequency of the probing AC electric current of 5000 Hz and calculation of the concentration of potassium ions is carried out by applying the formula: SC=3,0793-0,009*(Z1000/f1000)-0,00001274*(Z1000/f1000)^2, where ck is the concentration of potassium ions in mmol/l, Z1000 - the value of |Z| at the frequency of the probing AC electric current of 1,000 Hz, f1000 - value of the angle φ on the frequency of the probing AC electric current of 1,000 Hz, and the result of calculation of concentrations of biological substances in the serum is displayed and can be printed on the printer.
FIELD: measurement equipment.
SUBSTANCE: group of inventions relates to the field of biochemistry. A biosensor system and test sensors (versions) are proposed to determine concentration of an analysed substance in a sample. The biosensor system includes a test sensor, comprising at least two electric conductors, a reaction agent containing a binding substance, including at least one water soluble polymer material, buffer salt, a water soluble medium for transfer of one or two electrons, containing at least 20% (wt/wt) of inorganic salt of non-transition metal, a fermentative system and a non-ionic surfactant. The biosensor system also comprises a measurement facility to measure speed of oxidation-restoration reaction of the analysed substance.
EFFECT: proposed group provides for accurate determination of dependence of an output signal of a test sensor, which contains compositions of reagent with low total concentration of salt, from concentration of analysed substance in samples of whole blood in a wide range of levels of hematocrit within the limits of not more than 7 seconds.
49 cl, 9 dwg, 7 tbl
FIELD: measurement equipment.
SUBSTANCE: group of inventions relates to an analysis of biological liquids of a different nature. A method for determining concentration of an analyte in a sample involves stages at which at least one output signal value is generated, which depends on concentration of the analyte in the sample; at least one value ΔS is determined from at least one error parameter; at least one value ΔS represents deviation of an inclination or deviation of a normalised inclination relative to at least one basic correlation; at least one above said value of the output signal is compensated by means of at least one basic correlation and at least one value ΔS and concentration of the analyte is determined in the sample from at least one above said value of the output signal. Besides, the invention presents a biosensor system for determination of concentration of the analyte in the sample and sensor platinum for this biosensor system.
EFFECT: achieving improvement of accuracy and reliability of an analysis.
33 cl, 32 dwg, 3 tbl
SUBSTANCE: invention can be used to determine the inner energy of biospecifically reacting suspension of volumetric agglutination reaction (VAR) with brucellosis or tularemia antibody solutions and suspensions of cells. For this purpose the measurement of temperature difference of test biospecifically reacting suspension of volumetric agglutination reaction and control biospecifically unresponsive suspension is carried out, in which the antibody solution or suspension of cell antigen is replaced by the heterologous ingredient.
EFFECT: invention provides the ability of quantitative determination in SI system physical units of Joules and non-system calories of magnitude of the inner energy and work, respectively, shown and carried out as a result of the biospecific interaction of the active sires of antibodies and antigens in VAR suspension.
1 tbl, 6 ex
FIELD: measurement equipment.
SUBSTANCE: invention may be used as a measurement system for non-invasive express diagnostics of multi-component biological media for detection of viruses, bacteria and other microorganisms. A microscope comprises a source of radiation, a focusing lens, a diaphragm and a cuvette for placement of the examined object, arranged along the optical axis, a matrix of photodetectors, an electronic-computing system, including a unit of processing, software and a PC. Additionally upstream the cuvette there is a filter to smoothen Gaussian distribution of a radiation beam and to produce even lighting along the beam cross section. The cuvette has a transparent flat input window. The output window of the cuvette has the shape of the hemisphere with a radius equal to the distance from the input to the output window of the cuvette. The matrix of photodetectors has the shape of the hemisphere, which is located in parallel to the output window of the cuvette, follows its shape and is rigidly connected to it.
EFFECT: preservation of identical light force along cuvette cross section and increased resolution of a DHM.
SUBSTANCE: invention refers to orthopaedics and represents a diagnostic technique for a degree of severity of acute postoperative knee synovitis. According to the invention, the degree of severity of acute postoperative knee synovitis is determined by a microscopic examination of synovial fluid taking into account cytosis, s neutrophil count, a lymphocyte count and a synoviocyte count in a synoviocytogram that enables diagnosing mild, moderate or severe knee synovitis.
EFFECT: invention provides increasing the accuracy and simplifying the diagnostic technique.
5 tbl, 3 ex
SUBSTANCE: invention relates to the field of instrumentation and may be used to analyse a specific component, contained in a sample, in particular, level of glucose in blood. An analysing device or a method of analysis is proposed, by means of which one may receive valid results of analysis even under conditions, when ambient temperature changes, thus saving a user from inconvenience. The analysing device (1) is equipped with a facility (13) of detection, which detects, whether the ambient temperature measured with the help of a temperature measurement facility (6), within the previously determined temperature range. The detection facility (13) is made in order to determine whether the ambient temperature is within the previously determined temperature range, even under conditions, when information related to the projected substance in the sample, may not be produced from the analysing device.
EFFECT: increased validity and accuracy of analysis data.
14 cl, 14 dwg
SUBSTANCE: invention relates to medicine and describes an enzymatic ink composition comprising an enzyme capable of selectively recognising glucose in a blood sample, a mediator, and a first and a second fumed silica, wherein the first fumed silica has a BET surface area of about 130 to 170 m2/g and carbon content of about 0.8 to about 1.23 wt % and the second fumed silica has a BET surface area of about 270 to 330 m2/g and carbon content of about 1.4 to about 2.6 wt %.
EFFECT: enzymatic inks according to the present invention enable the use of a more advanced method of producing batches of test strips with a single calibration code and high efficiency.
12 cl, 6 dwg, 2 tbl, 6 ex
SUBSTANCE: invention can be used for the purpose of the early prediction of a risk of peripheral vitreochorioretinal dystrophies (PVCRD) on a paired eye after regmatogenous retinal detachment (RRD) surgeries. The IL18 content in the sub-retinal fluid (SRF) taken intraoperatively from an RRD eye is measured; if the measured value makes 550 pcg/ml, the PVCRD is predicted on the paired eye.
EFFECT: invention enables the preventive treatment of the paired eye in case of the risk of the developing PVCRD before the clinical manifestations of the developing PVCRD in the individual with operated retinal detachment on the other eye.
SUBSTANCE: invention relates to a method of forming a 3-phenylimino-3H-phenothiazine mediator or a 3-phenylimino-3H-phenoxazine mediator, which includes providing a first reactant including phenothiazine or phenoxazine, providing a first solvent, providing a second reactant and providing a second solvent. The first reactant, first solvent, second reactant and second solvent are combined to form a reactant solution. Sodium persulphate is added to the reactant solution to link the first and second reactants resulting in a reaction solution containing the 3-phenylimino-3H-phenothiazine or the 3-phenylimino-3H-phenoxazine mediator. Further treatment of the reaction solution is carried out to remove the first solvent and second solvent in order to separate the mediators. The invention also relates to products obtained using said method and use thereof in an electrochemical test sensor.
EFFECT: disclosed mediators have a low background current and have the required characteristics, including stability.
12 cl, 1 tbl, 25 ex, 5 dwg
SUBSTANCE: set of inventions relates to measurement of volume or concentration of biological matters. Proposed measuring device comprise casing and at least one display integrated therein. Note here that said casing comprises compartment for fitting the cartridge in said casing to fit specimen to be measured. Note also that said compartment has hole made at casing front. Note that casing first part for cartridge to be fitted therein extends at the angle to casing second part. Besides, this invention covers the method of measurement with the use of described device in analysis of biological fluids.
EFFECT: higher reliability, enhanced performances.
8 cl, 5 dwg
SUBSTANCE: invention relates to the field of analytical chemistry and can be used for automatic or express-analysis in laboratory or industrial conditions. The method of controlling main components of a chloroaluminate melt includes the determination of the molar ratio of the said components in the liquid chloroaluminate melt by a potentiometric method; measurement of the potential difference is carried out between a working electrode of aluminium and an aluminium comparison electrode both located in an electrolyte , with the comparison electrode being separated by a diaphragm from the main melt and representing an assembly from a ceramic casing and aluminium wire, submerged into the located in a casing chloroaluminate melt, saturated by solid potassium chloride, with the molar ratio of the main components in the melt being determined by the value of difference of potentials between the electrodes by means of preliminarily built calibration curves in coordinates "difference of potentials - ratio of concentrations of potassium chloride to aluminium chloride".
EFFECT: compactness of the installation, cheapness of the applied material and simplicity of technological equipment, applied for the said method realisation.
2 cl, 2 ex, 2 dwg
SUBSTANCE: invention relates to the electrochemical processor which comprises: a) first electrode and the second electrode, each of which has the first and opposite second surfaces, and the first electrode and the second electrode have the differing electrode potentials and physically separated mutually along the axes X, b) electrolyte, which covers at least a part of the first surface of the first electrode and a part of the first surface of the second electrode along the axis Y and electrically connects the named first electrode with the second electrode. Meanwhile at least a part of the first surface of the second electrode not covered by electrolyte, is covered towards the axis Y with a an electric insulation material, which bounds with an electrolyte, and the activation of the electrochemical processor favours to lateral foliation of the second electrode and isolation material, starting from the place of contact of the second electrode and isolation material, that ensures the channel between the second electrode and the isolation material. Besides, the invention relates to the use of this electrochemical processor and method of assembly of such electrochemical processor.
EFFECT: processor ensures the constant time of reaction.
17 cl, 12 dwg
SUBSTANCE: invention relates to analytical chemistry. The method is characterised by electrochemically concentrating benzoic acid on the surface of a graphite electrode for 90 s at electrolysis potential of (-0.500) V on a background of 0.1 mol/l sodium hydrogen phosphate, recording polarisation curves with linear potential sweep rate of 25 mV/s and determining concentration of benzoic acid from the peak height in potential range of 0.5-1.6 V relative to a silver chloride electrode.
EFFECT: method provides highly sensitive and rapid determination of benzoic acid in medicinal drugs.
3 ex, 6 tbl
SUBSTANCE: device for determining the oxygen and hydrogen concentration in a gas medium relates to measurement instruments and can be used for control of gas media parameters, in particular of those, containing oxygen and hydrogen. The device consists of a channel (7), located at an angle to the horizon, an input hydrogen sensor (2) and an input oxygen sensor (3), located in the input part of the channel (7) cavity, an input catalytically active element (1), installed in the channel (7) cavity above the output sensors of hydrogen (2) and oxygen (3), an output hydrogen sensor (5) and an output oxygen sensor (6), located in the channel (7) cavity between the input (1) and output (4) catalytically active elements. The input (2) and output (4) catalytically active elements are made of highly porous cellular materials with a platinum coating applied on their surface. As the input hydrogen sensor (5) and the output hydrogen sensor (7) used are solid electrolyte sensors of the hydrogen concentration with a ceramic sensitive element, made of oxygen-conducting ceramics.
EFFECT: increased speed of the device performance and sensitivity, provision of protection against erroneous readings of the device.
3 cl, 1 dwg
SUBSTANCE: invention relates to the field of analytic chemistry an can be applied for the simultaneous determination of the content of Cu(II), Pb(II), Fe(III) and Bi(III) ions in different matrices. The essence of the invention: a method of the combined determination of Cu(II), Pb(II), Fe(III) and Bi(III) ions by a method of capillary zone electrophoresis includes preliminary complex-formation of the components of a sample with ethylenediaminetetraacetic acid, introduction of the sample zone into a non-modified quartz capillary, filled with a phosphate buffer electrolyte, after a zone of diglycylglycine, separation of the components at negative polarity of a voltage source, direct spectrophotometric detection at a wavelength of 260 nm, identification and quantitative determination of each component by a preliminarily built calibrating diagram or by a method of standard additives.
EFFECT: extended list of determinable components, development of a simple, sensitive and express method of determining ions of transition metals in environmental objects, wastes of industries, complex oxide materials and other objects by the method of capillary zone electrophoresis.
FIELD: measurement equipment.
SUBSTANCE: invention relates to the field of engineering, which can perform remote monitoring of formation and growth of cracks in steel structures. A device includes a cover that has magnetic legs for attachment of the cover to a ferromagnetic structure, at least one pair of microprocessor-controlled voltage controls; with that, each voltage control has a wire of a sensor to an electrochemical fatigue sensor attached to the structure subject to an analysis for availability of cracks as a result of fatigue of metal in the steel structure, a power source and earthing; the voltage control is used to perform monitoring of fatigue state of the steel structure; each voltage control is electrically isolated from the rest part of an electrical mounting plate of the device and includes an analogue-to-digital converter. Voltage is applied by means of a voltage control through the above sensors during cyclic loading, and current flowing through the above sensors is measured, and the measurement result is digitised for the analysis of fatigue state of the steel structure.
EFFECT: possible prevention of occurring noises and distortion of data during the measurement.
34 cl, 4 dwg
SUBSTANCE: invention relates to the field of electrochemical methods of analysis, in particular to the analysis of solutions to determine the total antioxidant/oxidant activity. The invention can be used in research laboratories, food industry, medicine to determine the antioxidant/oxidant activity of natural, synthetic and biological objects to study antioxidant/oxidant properties of substances and products, control the composition of food products, and diagnose diseases. The essence of the claimed method consists in the following: determination of the antioxidant/oxidant activity is carried out by a difference of potentials, one of which is measured after the realisation of a chemical reaction between antioxidants/oxidants of an analysed substance and an applied agent, and the second one - after the following addition of a solution of a reagent or the analysed substance.
EFFECT: invention provides an increase of accuracy, reliability and reproducibility of results, increase of the analysis expression, extension of the range of analysed substances, applied reagents and solvents.
3 cl, 6 dwg, 6 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine and represents a reagent for detecting glucose containing an enzyme FAD-glucose dehydrogenase, a phenothiazine or phenoxazine mediator, at least one surfactant, a polymer and a buffer. The reagent is used with an electrochemical test sensor comprising a number of electrodes with the test sensor possessing high filling rate and accuracy.
EFFECT: reagent is stable and characterised by lower background current of the test sensors.
19 cl, 2 tbl, 13 dwg, 9 ex
SUBSTANCE: method involves extraction preparation of a sample of biological material, centrifuging and performing analysis on a capillary electrophoresis system in a quartz capillary with an effective length of 0.5 m and inner diameter of 75 mcm, wherein analysis is performed using an aqueous main electrolyte which contains 0.28% boric acid and 0.04% sodium tetraborate, with positive voltage polarity and detection wavelength of 254 nm.
EFFECT: rapid and reliable quantitative determination of indolyl-acetic acid by capillary electrophoresis using nontoxic and readily available reactants for analysis.
6 ex, 1 tbl, 1 dwg
SUBSTANCE: method involves potentiometric titration of a sample with complexon (III) with an indicator electrode made of bismuth metal with a buffer solution at pH 4.1-9.0.
EFFECT: easier, more accurate and safer analysis.
2 tbl, 1 dwg
SUBSTANCE: method consists in diagnosing chronic cardiac failure. The diagnostic procedure involves using the high-frequency electrical impedance analysis. Bipolar measurements of electrical impedance of the chest involve recording average modular impedance |Z| and phase angle φ, and calculating the relation |Z|/|φ|. The measurements taken involve probing with AC current at frequencies 50, 100, 200 and 500 kHz. That implies using electrocardiographic electrodes 21 mm in diameter. The first electrode is placed in the 3rd intercostal space along the left parasternal line. The second one is placed series in three positions. For the first time, the second electrode is placed in the 2nd intercostal space along the left sternal line, lead 3-2. Then, in the 3rd intercostal space along the right parasternal line, lead 3-3. Thereafter in the 5th or 6th intercostals space on the left in a projection of apex beat, lead 3-5. Decreasing angle φ modulus at frequency 200 kHz in lead 3-2 less than 34°, and/or increasing the relation |Z|/|φ| measured at frequency 200 kHz in lead 3-3 more than 15, and/or decreasing the relation |Z|/|φ| measured at frequency 200 kHz in lead 3-5 less than 10 enable diagnosing chronic cardiac failure.
EFFECT: method increases the diagnostic accuracy ensured by measuring combined with recording the above parameters.