Coloured x-ray contrast mass for filling vessels and method for preparation thereof for anatomical studies
SUBSTANCE: coloured X-ray contrast mass consists of barium sulphate, glycerine, acrylic dye and aqueous gelatine solution. The invention relates to methods of producing a setting coloured X-ray contrast mass. The disclosed mass can be used for macro- and micro-preparation of vessels and X-ray imaging thereof. The preparation method includes thoroughly mixing barium sulphate, glycerine, acrylic dye and aqueous gelatine solution, wherein barium sulphate, glycerine, acrylic dye and aqueous gelatine solution are taken in amount of 1.5 parts, 1 part, 0.1 parts and 7.5 parts, respectively, stirring and heating the mixture to 60-80°C before application. The prepared mass has high permeability in thin vessels, brightness (clarity), fast setting and elasticity. The mass is prepared from readily available and cheap materials. The remaining or unwanted mass can be stored for a long period of time.
EFFECT: disclosed mass can be heated repeatedly and is ready for use.
The present invention relates to the field of humane medicine, veterinary medicine, biology, namely to methods for preparing colored paste cordials vascular system in anatomical studies. Injection of colored masses greatly facilitate macro - and Microdissection, as filled with contrast vessels serve as a good guide and better identified in the investigated post mortem material. X-rays (angiography) allows to study the angioarchitectonics and topography of blood vessels, is not modified during the dissection, and further serves as a landmark in the study of the bloodstream to organs and tissues. To study the normal and pathological morphology of cardiovascular system there was always the question of the relevance of methods of filling contrasting masses of blood vessels.
Injection mass can be attributed to dyeing hot filling substances for blood and (or) venous vessels with radiopaque properties. The proposed lot can be used for macro - and micropreparative vessels and their x-rays.
The prior art discloses similar compositions and method of preparation of the filling mass.
Known, for example, injecting radiopaque mass to fill the vessels, consisting of 20-25% of barium sulfate 5% solution of gelatin or nutricosmetics, heated to a temperature of +38°C (Shalimov S. A., Radzikhovsky, A. P., and Kasevich L. V. - M., Medicine, 198, p. 95).
The disadvantage is a small shelf life of ready mass, poor permeability in vessels of small calibre and lack of staining, making it difficult dissection.
Similar in composition and method of producing colored filling mass is solidified "hot weight" Herlihy-Gooyer, consisting of a 10% gelatin solution mixed with sifted chalk or tooth powder and paint, which is injected into the vessels at a temperature of 38°C (Khrustalev, I. V. techniques of manufacturing and storage of anatomical specimens with the basics of museology / I. V. Khrustalev, Khristoforova B. V. - M., IBA, 1986, p. 42).
The disadvantage of rentgencontrastnoe vessels, low penetration rate in small vessels due to increased density of the mass and the lack of time of preservation of mass in usable condition.
Known method of preparing a colored paste cordials vascular system by the anatomical researches on patent No. 2423702 IPC G01N 33/49, CL A01N 1/00, publ. 10.07.2011, which includes pre-mixing of the dye (water-soluble acrylic paint) with water in a ratio of 0.9 to 1.1 parts by 0,18-0,21 parts until a homogeneous mass, add the 9.7-9.9 parts of water and 0.18-21 parts 96% ethanol, incubated for 12 hours filterout.
The disadvantage is the lack of rentgencontrastnoe mass, as well as the need soaking for 12 hours.
A disadvantage of known methods is the provision of only one of the necessary for the study of blood vessels properties. So, earlier known recipe of contrasting masses stained vessels for preparation or only provide radiopacity with loss of visualization of surrounding tissues. Weak permeability in small vessels, the difficulty of preparation and lack of time of preservation of mass in the prepared state.
The closest analogue (prototype) is a method for preparing a color contrast mass cordials vessels (Parables V. A. Comparative and age-related macro - and micromorphology of the arterial bed of thin and thick departments of intestines of sheep and goats. Dis. Stavropol, 2005, p. 81), which consists in the fact that they use a lot consisting of carcasses with glycerin, barium sulfate, lead minium and white lead, in which dry barium sulfate was preliminarily pulverized in a mortar, sifted through a sieve, diluted by gradual addition of glycerol at constant rubbing to the consistency of cream, then in a mortar was filled with distilled water, after which the slurry was filtered, and before use was usbantivirus.
What ka�recover it this way contrast weight fills not only microscopically visible vessels, but smaller, up to arterioles and venules with the possibility of x-ray examination.
The disadvantage is the insufficient brightness of the filled vessels, making their differentiation from surrounding tissue, and if the damage to the integrity of their contrasting mass flows and "pollutes" the material studied.
The object of the invention is to increase the brightness of stamina, increase the retention of operating properties in the prepared condition colored radiopaque mass cordials vessels and method of preparation for anatomical study
The problem is solved by adding in a colored mass of gelatin solution, which makes a lot more durable and elastic, on the retention properties of the mass, and the brightness of the existence, also affected by the application of a solution of an acrylic paint.
The invention consists in that the barium sulfate are thoroughly mixed with glycerin and acrylic paint until smooth (add acrylic paint adds color and brightness contrast weight, and further vessels respectively), then poured into an aqueous solution of gelatin (gelatin does not spread weight when damage to the integrity of the vessel without loss and� elasticity) and heated to 60-80° to the introduction, and the ratio of ingredients in parts corresponds to the barium sulfate is 1.5 parts of glycerol, 1 part acrylic paint - 0,1 part and an aqueous solution of gelatin and 7.5 parts. The mass thickens quickly in the blood vessels (due to the gelatin solution) and requires a small amount of time to start preparation after it fills. Also there is no need to withstand the weight after cooking, and you can immediately start pouring it into the vessels and then to the dissection.
Method of preparing colored radiopaque mass cordials vessels is implemented as follows.
A specific example of preparation of the masses
Powder of barium in the form of insoluble sulfate salt was ground in a ceramic dish.
It pounded the barium sulfate was added glycerin in the proportion 1.5 parts by BaSO4to 1 part of glycerin and thoroughly mixed to obtain a homogeneous mass, viscous creamy consistency.
To the resulting mass was added acrylic paint in a small amount is 0.1 part (i.e. 1-3 g enough to obtain a homogeneous saturated bright mass). Acrylic paints are characterized by high luminosity, waterproof and temperature resistance, tight grip, dry quickly, do not crack and have high strength.
Then 10-15 g of gelatin poured warm (20-30°C) water 200-25 ml and left for 15 min to swell, stirring occasionally (aqueous gelatin solution is about 7.5 parts of the total weight).
Swollen granules of gelatin was heated on the water bath at 60-80°C until full dissolution and the production of liquid smooth.
The hot aqueous solution of gelatin was poured to the weight of barium sulphate, glycerine and acrylic paints, then thoroughly mixed until a homogeneous liquid mass.
The ratio of parts by mass of the identified experimentally.
Add in colored radiopaque mass of acrylic helps make it more brightness, longer preservation, and gelatin, in addition, gives a lot of flexibility and also the ability to save over a long period of time, the prepared weight in working condition.
The goal is to create easy to prepare and the use of contrast mass, consisting of inexpensive and readily available substances, highly informative post-mortem method of research of the cardiovascular system in humans and animals and the use of the autopsy of the drug in the training and demonstration process for a long time, made.
The sum of all applied in the mass of ingredients in the above proportions, prepared by the proposed method allows to solve the task.
1. Renganathan�age mass of color cordials vessels for anatomical study, containing a mixture of barium sulfate and glycerin, characterized in that a mixture of acrylic paint and an aqueous solution of gelatin.
2. Method of preparing colored radiopaque mass cordials vessels for anatomical study, characterized by the fact that in the mixture of barium sulfate and glycerol was added acrylic paint and gelatin, wherein barium sulfate take 1.5 parts of glycerol, 1 part acrylic paint and 0.1 parts of an aqueous solution of gelatin is added 7.5 parts, stirred, and before applying the mixture is heated to a temperature of 60-80°C.
SUBSTANCE: what is involved is a morphological examination aimed at observing mantle-free lymphoid follicles and surface epithelium infiltration with intraepithelial lymphocytes with lysed zones in a proper gastroduodenal mucous plate; if any, virus-associated gastroduodenitis is diagnosed.
EFFECT: technique enables optimising the screening procedure of virus-associated chronic gastroduodenitis in children.
SUBSTANCE: invention refers to diagnostic technique in medicine and describes a laboratory diagnostic technique for Alzheimer disease by using metal-binding domain modified 1-16 forms of human beta-amyloid as Alzheimer disease pathogenesis biomarkers. The method is characterised by the fact that total peptide fraction, then beta-amyloid fraction and fragments of 1-16 beta-amyloid fraction to be further analysed after the targeted proteolysis is recovered from a denatured sample of biological fluid, urine, cerebrospinal fluid. If the biological fluid sample appears to contain a metal-binding domain modified, asparlic acid isomerised (position 7) form of beta-amyloid in the relative amount of more than 5% of the total amount of the 1-16 beta-amyloid fraction and/or a metal-binding domain modified, serine phosphorylated (position 8) form of beta-amyloid, Alzheimer disease is diagnosed.
EFFECT: invention can be used to establish a diagnosis of Alzheimer disease.
SUBSTANCE: portion of endothelial cell culture is pre-processed with immunoglobulins for intravenous administration, while the other portion is left unprocessed, cultured with female's blood serum to measure a percentage of non-viable endothelial cells processed with immunoglobulins for intravenous administration. A percentage of non-viable endothelial cells unprocessed with immunoglobulins for intravenous administration is also determined. The cytoprotective effect is calculated in per cent by formula: CE=100-100X/X1, wherein X is the percentage of non-viable endothelial cells processed with immunoglobulins after culturing with blood serum; X1 is the percentage of non-viable endothelial cells unprocessed with immunoglobulins after culturing with blood serum. If the derived value is more than 55%, the cytoprotective effect is considered to be positive.
EFFECT: method enables predicting the cytoprotective effect of intravenous immunoglobulins both in treating the females suffering recurrent pregnancy loss and diagnosed anti-phospholipid syndrome, and in treating some other diseases related to endothelial injuries and derived endothelial dysfunction.
6 dwg, 3 tbl, 2 ex
SUBSTANCE: invention refers to a method of simultaneous determination of zinc cation concentration and zinc and copper cation ratio in blood serum, involving use of dithizone solution in carbon tetrachloride as the main reactant, incubation at room temperature in alkali medium, organic phase removal and colorimetry of colloid solutions of blood serum sample and standard solution at 535 nm, zinc cation concentration calculation, colorimetry of colloid solutions of blood serum sample and standard solution at 435 nm and molar ration calculation for zinc and copper cations.
EFFECT: determination of zinc cation concentration in blood serum together with zinc and copper cation ratio in the same sample without additional reactants.
FIELD: veterinary medicine.
SUBSTANCE: essence of the proposed method of instant diagnosis of genital form of infectious rhinotracheitis (IRT) in cows is treatment of surface of cervix uteri with 3% acetic acid at a dose of 10-15 ml, removal of surface mucus and identifying areas of mucosa with atypical cells which become white during the test. Upon condition of appearance of white coloured spots the laboratory tests of blood samples of the cow are performed, to confirm the presence or absence of IRT virus in animals according to known methods.
EFFECT: method enables for 4-5 minutes with high certainty to diagnose the presence of genital form of infectious rhinotracheitis in cows.
SUBSTANCE: following parameters are determined for the purpose of detection andtimely correction of a immune metabolic polypathy in females aged 19-22 years old and males 25-30 years old: blood pressure, heart rate, waist circumference (WC), hip circumference (HC), WC/HC ratio, body weight index, lipid profile, uric acid, thyroid-stimulating hormone (TSH), insulin, leptin, prolactin, testosterone, sex hormone binding globulin (SHBG), luteinising hormone (LH), follicle-stimulating hormone (FSH), anti-mullerian hormone (for females). If any of the parameters vary by 10-25% from standard, the person being tested is referred to a risk of the immune metabolic polypathy requiring preventive measures. If three or more parameters vary significantly - more than 25% - from the norm, the presence of the existing polypathy requiring conducting the therapeutic measures by a specialist.
EFFECT: method provides the most accurate determination of a transition moment of the physiological metabolic processes into physiological ones as an indicator of disturbed homeostasis and pre-nosological body state in the young individuals.
2 cl, 1 tbl, 1 ex
SUBSTANCE: method of detection of intracellular proteins using fluorescein-5-isothiocyanate (FITC) in various types of mammalian cells is provided, which are characterised by different levels of the synthetic process, using the method of confocal laser microscopy. The mammalian cells are fixed by paraformaldehyde (PFA) preventing the extraction of proteins in subsequent stages of staining, permeabilised with detergent, then stained for 2 h with FITC in a concentration of 1 mcg/ml. The cells are enclosed in Mowiol 4-88 adding DABCO (1,4-diazabicyclo[2.2.2] octane). The resulting preparations are used to analyse the localisation and the content of the protein component of the cells by confocal microscopy.
EFFECT: invention enables to obtain information and to investigate the location of proteins in the cells and to carry out a comparative analysis of protein content in cells with different levels of metabolism.
7 dwg, 7 ex
SUBSTANCE: technique involves intestinal ultrasonography and X-ray imaging, peripheral computed electrogastroenterography and bacteriological faeces analysis. Besides, at least 24 hours from the onset of acute pancreatitis and at least 24 hours after, faeces is sampled for microscopic study; if the findings show a large number of cylindrical epithelium cells in the faeces, acute pancreatitis is considered to be developing.
EFFECT: technique enables performing the non-invasive diagnosing of intestinal failure in the patients suffering abortion-induced acute pancreatitis.
SUBSTANCE: invention refers to biology and medicine, namely to laboratory methods of examination. Detecting phosphatidyl serine on the erythrocyte membrane surface is ensured by fixing cells by glutaric aldehyde, washing in distilled water and placing on a slide processed with lanthanum chloride, mixing and studying cell aggregation by optical microscopy. Observing the erythrocyte aggregates testifies to the presence of phosphatidyl serine on the erythrocyte membrane surface.
EFFECT: invention provides developing simple, fast method requiring no special equipment and critical agents and used for detecting phosphatidyl serine on the erythrocyte membrane surface.
SUBSTANCE: method of the postnatal diagnostics of foetal alcohol syndrome in deceased young children consists in the following: the brain of the deceased child is weighed and its weight is fixed. Morphological changes in the brain are identified. For this purpose blocks of the brain substance are cut out from the convexital cortex of the sensomotor zone, hypothalamic zone and cerebellum. Analysis by light-optical microscopy with the preliminary staining of the brain cuts is carried out. The staining is performed with hematoxylin-eosin by Van Gieson and by Nissl. Results of the analysis are used to diagnose foetal alcohol syndrome in case if a complex of the following morphological changes of the brain is present: in the brain substance from the convexital cortex of the sensormotor zone there are sections of rarefaction in middle layers, chromatolysis of the cytoplasm of the nerve cells and pyknotic changes, in the brain substance of the hypothalamic area there is chromatolysis of the nerve cells in the form of the Nissl substance margination, pericellular oedema, in the brain substance of the cerebellum there is the rarefaction of cells, dystrophic changes of the Purkinje cells, with the fixed weight of the brain being lower than the minimally admissible for a normally developed child of the similar age.
EFFECT: increase of reliability and extension of the arsenal of means of forensic examination for the postnatal diagnostics of foetal alcohol syndrome in deceased young children.
3 dwg, 1 ex
SUBSTANCE: multispiral computed tomography and intravenous angiography in varicose vein disease that is ensured by catheterising a saphena of the examined lower extremity with introducing a non-ionic radiographic pigment therein. That is followed by scanning with forming a three-dimensional image of the veins by means of automatic three-dimensional rendering protocols included into a multispiral computed imaging scanner. The scanning procedure is sequential and two-staged, wherein the first scanning stage is initiated 20 seconds after the radiographic pigment has been introduced with a 30-second breath holding; the second stage of scanning is performed 60 seconds later with the patient breathing freely. The first scanning procedure is directed from the foot to pelvis, while the second one - from the pelvis to foot.
EFFECT: more effective diagnosing of the anatomy of the lower extremity venation by facilitating the interpretation of the obtained results, visualisation, higher quality of the vein image 3D-reconstruction with adequate and uniform filling of the lower extremity venous bed with the radiographic pigment.
1 dwg, 1 ex
SUBSTANCE: composition contains Compound I a pharmaceutically acceptable carrier and dissolved sodium compound and calcium compound providing the sodium ion concentration of 40-50 mM and the calcium ion concentration of 0.1-0.7 mM. The present invention also refers to imaging methods using such diagnostic composition.
EFFECT: invention describes the X-ray diagnostic composition, which exhibits the excellent cardiac safety profile.
16 cl, 6 dwg, 3 ex, 5 tbl
SUBSTANCE: X-ray examination of a venous bed of an individual's rectum involves using 76% urografin. The inferior mesenteric vein stem and iliac vascular bundles from both sides are approached. They are catheterised and prepared by washing in 10% ammonia and distilled water. Before preparation, the inferior mesenteric vein is detected by a method of visualisation of the portal vein system. A green solution of brilliant green is inserted into its stem. The final stage is a direct X-contrast examination by fixed 12P9 Armobil-9 X-ray apparatus. 3 series of images are done. The first comparison X-ray represents plan radiography in a frontal projection of the small pelvis. The second X-ray is plan radiography in a frontal projection of the small pelvis with tight filling of the prepared bed of the rectal portal system with X-ray contrast 76% urografin. The third X-ray is plan radiography in a frontal projection of the small pelvis with tight filling of the internal iliac veins from both sides in a combination with X-ray contrast 76% urografin.
EFFECT: method provides optimising the X-ray examination of the individual's rectal veins, visualising the vascular system adequately, ensuring the information concerning special characteristics of the vascular organometry, enables detecting the portocaval anastomosis by the optimum preparation of the vascular system, using urografin as the contrast substance and implementing the developed procedure of the X-ray examination.
3 dwg, 1 ex
SUBSTANCE: invention relates to a method of producing paramagnetic iron oxide nanoparticles. The disclosed method includes reacting an iron complex having iron as a central atom and a carboxylate group having 10 to 22 carbon atoms which is bonded to the central atom in a ligand form; a C10-C22 fatty acid and a C10-C22 aliphatic alcohol or C10-C22 aliphatic amine to obtain iron oxide nanoparticles. The iron oxide nanoparticles are obtained by raising the temperature from room temperature to 200 to 250°C with at a rate of 5°C/min or higher, and conducting the reaction at 200 to 250°C for 5 to 60 min.
EFFECT: invention enables to obtain iron oxide nanoparticles having a size of 4nm or less, which can be used as a MRI T1 contrast agent.
7 cl, 1 tbl, 25 dwg, 31 ex
SUBSTANCE: invention refers to medicine, oncology, radiodiagnostics of nonpalpable intraductal benign tumours and intraductal breast cancer manifested by nipple discharge and not presented by mammography and ultrasonic examination. The method involves a stereotaxic percutaneous invasive tumour labelling; that is ensured by enhancing the tumour first by filling the ductal system with an urographin solution; a ductograpm is derived in a frontal projection, and mutually perpendicular lines are drawn thereon - through a tumour centre in the frontal projection and through a nipple in a sagittal plane. That is followed by measuring lengths of two segments: from the nipple to a cross point of these lines and from a lateral or medial edge of the gland depending on the tumour localisation in an inner or outer quadrant, to the tumour. The drawn segments are marked on the skin by protracting the corresponding distance from the nipple and from the edge of the gland to the cross point thereof that is a point of the tumour projection on the skin. This marked point is used for the purpose of the stereotaxic localisation of the tumour by means of a wire localisation mandril.
EFFECT: method enables the reliable localisation of the pathological new growth in the mammary gland accurate within +0,1mm with ensured removal of the new growth by a segmental mastectomy.
1 dwg, 1 ex
SUBSTANCE: invention relates to medicine, diagnostics of adenoma of parathyroid glands (PTG), and can be applied in radiodiagnostics, endocrinology, surgery. Multi-phase multispiral computed tomography (MSCT) with X-ray contrast preparation (RC) is carried out at arterial and venous phases of analysis - respectively at 25 and 50 seconds after RC introduction. reading of parameters, analysis of obtained images and pre-operation diagnostics of patient's state are performed. RC is introduced single time, after its introduction physiological solution is introduced intravenously, with introduction of preparations through catheter at rate 4±1 ml/s. Visual and densitometric analysis of obtained images are performed in analysis of obtained images. As analysis parameters determined are: coefficient of radiation attenuation in all formation of the area under examination, suspicious of PTG adenoma. Presence of adenoma is diagnosed with fixation of X-ray radiation attenuation coefficient to +180+200 HU at early arterial phase and detection of not less than 80 HU density reduction at venous stage.
EFFECT: method provides improvement, high quality of diagnostics of PTG adenomas due to more accurate fixation of neoplasm location, optimal distribution and retention of contrast substance in the zone of examination in healthy and pathogenic zones, which makes it possible to reduce operation time to 15 minutes instead of 60 minutes, reduce post-operation period from 5 days to 1 day.
6 dwg, 4 ex
FIELD: veterinary medicine.
SUBSTANCE: method of production of roentgenopaque mass for vaso-X-ray radiography in post-mortem examinations of animals comprises preparation of the mass consisting of 45% white lead composed with 45% turpentine oil, and 10% of medical plaster powder, added as a thin jet in the given composition. At that the medical plaster powder is previously sieved, and the resulting mass is stirred vigorously for 20-30 minutes until obtaining the slurry of homogeneous consistency with a viscosity similar to blood plasma.
EFFECT: production of injection mass having high radiopacity, fine dispersion ability, the ability of precipitation on the walls of the vascular bed, accessible and affordable.
SUBSTANCE: invention relates to molecular imaging. The imaging system comprises a radiation source which crosses the investigated region, a detector for detecting radiation and generating a signal which characterises energy of the detected radiation, a data selector which performs signal discrimination according to energy based on energy spectrum-related settings which correspond to the first and second spectral characteristics of a contrast substance administered to a subject, and a unit for signal reconstruction based on the first and second spectral characteristics and generation of three-dimensional image data characterising a target. The contrast substance has a first spectral attenuation characteristic when bonded to a target and a second different spectral characteristic in a state without bonding to the target.
EFFECT: use of the invention increases the amount of the obtained information on the composition of the tissue of a subject.
10 cl, 14 dwg
SUBSTANCE: invention relates to a nanoparticle-based contrast agent, where the nanoparticles contain a core whose surface is silicon dioxide free and a coating bound to the surface of the core and contains a silane-functionalised zwitterionic group. In one version, the nanoparticle does not contain a ligand which facilitates directed delivery. In another version, the nanoparticle contains a ligand which facilitates directed delivery, wherein the zwitterionic group and the ligand which facilitates directed delivery are not identical. The invention also relates to methods of producing said nanoparticles, a contrast agent composition containing a plurality of nanoparticles and use of the nanoparticles as a diagnostic agent.
EFFECT: invention relates to methods of using a composition which contains a plurality of said nanoparticles for X-ray imaging, computed tomography (CT) or magnetic resonance imaging (MRI), where the method includes administering the composition to a subject and imaging the subject using a diagnostic device.
23 cl, 2 tbl, 6 dwg
SUBSTANCE: invention refers to medicine, pulmonology, and roentgenology. An X-ray diagnostic technique for open retention cysts of the tracheal and bronchial excretory glands consists in administering a contrast agent into dilated excretory ducts of the cysts. The contrast agent is presented by an air-powder aerosol of a bioinert material with particles of the diameter less than 1 mcm. Active inhalation provides the particles motion into each cyst through the excretory duct and the particles deposition on the cyst walls to visualise in such a way the presence thereof. The bioinert material is presented by tantalum, a powder of which is placed into a container coupled with PAI-2 portable aerosol inhaler comprising a compressor. An air spray is generated to activate the powder and to form the air-powder aerosol to be supplied through a catheter into the excretory ducts of the open retention cysts of the tracheal and bronchial excretory glands.
EFFECT: method provides accurate and reliable X-ray diagnosis of specific formations of the tracheobronchial tree - open retention cysts of the tracheal and bronchial excretory glands.
2 cl, 1 dwg, 1 ex
SUBSTANCE: cryoprotector is opened in a laminar flow unit; a special syringe of asyringe pump is filled with a cryoprotector; that is followed by introducing a filter solution of the cryoprotector - 55% dimethylsulphoxide with 5% dextran 40 at temperature +4°C in a nucleated cell suspension with haemopoietic stem cells in a cryopackage with the concentrate and mixing mechanically in a mixing apparatus, transferring the system together with the cryoprotector flask into the laminar flow unit; the air is released from the cryopackage and portion of the suspension; the package is sealed and placed into a shrink bag; that is followed by programmed multi-stage freezing, the first stage of which keeping the mixture of the suspension with the stem cells and cryoprotector - a freezing sample - for 10 min at temperature +4°C; the second stage is cooled at a rate of 1°C/min to temperature -12°C; the thirst stage provides cooling at a rate of 20°C/min to temperature -60°C; at the fourth stage, the sample is heated at a rate of 10°C/min to temperature -18°C; at the fifth stage, the sample is cooled at a rate of 1°C/min to -60°C; at the end of the freezing program, the sample is cooled at a rate of 3°C/min to temperature -100°C; after freezing, the sample is placed into a quarantine dewar with liquid nitrogen until infection and bacteriological fungal contamination test results are obtained. After termination of the quarantine shelf life, the sample with haemopoietic stem cells are placed for long-term storage at temperature not exceeding -150°C, into the dewar with liquid nitrogen if observing negative test results. If the infection and bacterial and/or fungal contamination test results are positive, the sample with haemopoietic stem cells are transferred into the dewar with liquid nitrogen for infectious material for long-term storage.
EFFECT: invention enables increasing cell viability in the sample.
3 cl, 4 dwg