Coloured x-ray contrast mass for filling vessels and method for preparation thereof for anatomical studies

FIELD: chemistry.

SUBSTANCE: coloured X-ray contrast mass consists of barium sulphate, glycerine, acrylic dye and aqueous gelatine solution. The invention relates to methods of producing a setting coloured X-ray contrast mass. The disclosed mass can be used for macro- and micro-preparation of vessels and X-ray imaging thereof. The preparation method includes thoroughly mixing barium sulphate, glycerine, acrylic dye and aqueous gelatine solution, wherein barium sulphate, glycerine, acrylic dye and aqueous gelatine solution are taken in amount of 1.5 parts, 1 part, 0.1 parts and 7.5 parts, respectively, stirring and heating the mixture to 60-80°C before application. The prepared mass has high permeability in thin vessels, brightness (clarity), fast setting and elasticity. The mass is prepared from readily available and cheap materials. The remaining or unwanted mass can be stored for a long period of time.

EFFECT: disclosed mass can be heated repeatedly and is ready for use.

2 cl

 

The present invention relates to the field of humane medicine, veterinary medicine, biology, namely to methods for preparing colored paste cordials vascular system in anatomical studies. Injection of colored masses greatly facilitate macro - and Microdissection, as filled with contrast vessels serve as a good guide and better identified in the investigated post mortem material. X-rays (angiography) allows to study the angioarchitectonics and topography of blood vessels, is not modified during the dissection, and further serves as a landmark in the study of the bloodstream to organs and tissues. To study the normal and pathological morphology of cardiovascular system there was always the question of the relevance of methods of filling contrasting masses of blood vessels.

Injection mass can be attributed to dyeing hot filling substances for blood and (or) venous vessels with radiopaque properties. The proposed lot can be used for macro - and micropreparative vessels and their x-rays.

The prior art discloses similar compositions and method of preparation of the filling mass.

Known, for example, injecting radiopaque mass to fill the vessels, consisting of 20-25% of barium sulfate 5% solution of gelatin or nutricosmetics, heated to a temperature of +38°C (Shalimov S. A., Radzikhovsky, A. P., and Kasevich L. V. - M., Medicine, 198, p. 95).

The disadvantage is a small shelf life of ready mass, poor permeability in vessels of small calibre and lack of staining, making it difficult dissection.

Similar in composition and method of producing colored filling mass is solidified "hot weight" Herlihy-Gooyer, consisting of a 10% gelatin solution mixed with sifted chalk or tooth powder and paint, which is injected into the vessels at a temperature of 38°C (Khrustalev, I. V. techniques of manufacturing and storage of anatomical specimens with the basics of museology / I. V. Khrustalev, Khristoforova B. V. - M., IBA, 1986, p. 42).

The disadvantage of rentgencontrastnoe vessels, low penetration rate in small vessels due to increased density of the mass and the lack of time of preservation of mass in usable condition.

Known method of preparing a colored paste cordials vascular system by the anatomical researches on patent No. 2423702 IPC G01N 33/49, CL A01N 1/00, publ. 10.07.2011, which includes pre-mixing of the dye (water-soluble acrylic paint) with water in a ratio of 0.9 to 1.1 parts by 0,18-0,21 parts until a homogeneous mass, add the 9.7-9.9 parts of water and 0.18-21 parts 96% ethanol, incubated for 12 hours filterout.

The disadvantage is the lack of rentgencontrastnoe mass, as well as the need soaking for 12 hours.

A disadvantage of known methods is the provision of only one of the necessary for the study of blood vessels properties. So, earlier known recipe of contrasting masses stained vessels for preparation or only provide radiopacity with loss of visualization of surrounding tissues. Weak permeability in small vessels, the difficulty of preparation and lack of time of preservation of mass in the prepared state.

The closest analogue (prototype) is a method for preparing a color contrast mass cordials vessels (Parables V. A. Comparative and age-related macro - and micromorphology of the arterial bed of thin and thick departments of intestines of sheep and goats. Dis. Stavropol, 2005, p. 81), which consists in the fact that they use a lot consisting of carcasses with glycerin, barium sulfate, lead minium and white lead, in which dry barium sulfate was preliminarily pulverized in a mortar, sifted through a sieve, diluted by gradual addition of glycerol at constant rubbing to the consistency of cream, then in a mortar was filled with distilled water, after which the slurry was filtered, and before use was usbantivirus.

What ka�recover it this way contrast weight fills not only microscopically visible vessels, but smaller, up to arterioles and venules with the possibility of x-ray examination.

The disadvantage is the insufficient brightness of the filled vessels, making their differentiation from surrounding tissue, and if the damage to the integrity of their contrasting mass flows and "pollutes" the material studied.

The object of the invention is to increase the brightness of stamina, increase the retention of operating properties in the prepared condition colored radiopaque mass cordials vessels and method of preparation for anatomical study

The problem is solved by adding in a colored mass of gelatin solution, which makes a lot more durable and elastic, on the retention properties of the mass, and the brightness of the existence, also affected by the application of a solution of an acrylic paint.

The invention consists in that the barium sulfate are thoroughly mixed with glycerin and acrylic paint until smooth (add acrylic paint adds color and brightness contrast weight, and further vessels respectively), then poured into an aqueous solution of gelatin (gelatin does not spread weight when damage to the integrity of the vessel without loss and� elasticity) and heated to 60-80° to the introduction, and the ratio of ingredients in parts corresponds to the barium sulfate is 1.5 parts of glycerol, 1 part acrylic paint - 0,1 part and an aqueous solution of gelatin and 7.5 parts. The mass thickens quickly in the blood vessels (due to the gelatin solution) and requires a small amount of time to start preparation after it fills. Also there is no need to withstand the weight after cooking, and you can immediately start pouring it into the vessels and then to the dissection.

Method of preparing colored radiopaque mass cordials vessels is implemented as follows.

A specific example of preparation of the masses

Powder of barium in the form of insoluble sulfate salt was ground in a ceramic dish.

It pounded the barium sulfate was added glycerin in the proportion 1.5 parts by BaSO4to 1 part of glycerin and thoroughly mixed to obtain a homogeneous mass, viscous creamy consistency.

To the resulting mass was added acrylic paint in a small amount is 0.1 part (i.e. 1-3 g enough to obtain a homogeneous saturated bright mass). Acrylic paints are characterized by high luminosity, waterproof and temperature resistance, tight grip, dry quickly, do not crack and have high strength.

Then 10-15 g of gelatin poured warm (20-30°C) water 200-25 ml and left for 15 min to swell, stirring occasionally (aqueous gelatin solution is about 7.5 parts of the total weight).

Swollen granules of gelatin was heated on the water bath at 60-80°C until full dissolution and the production of liquid smooth.

The hot aqueous solution of gelatin was poured to the weight of barium sulphate, glycerine and acrylic paints, then thoroughly mixed until a homogeneous liquid mass.

The ratio of parts by mass of the identified experimentally.

Add in colored radiopaque mass of acrylic helps make it more brightness, longer preservation, and gelatin, in addition, gives a lot of flexibility and also the ability to save over a long period of time, the prepared weight in working condition.

The goal is to create easy to prepare and the use of contrast mass, consisting of inexpensive and readily available substances, highly informative post-mortem method of research of the cardiovascular system in humans and animals and the use of the autopsy of the drug in the training and demonstration process for a long time, made.

The sum of all applied in the mass of ingredients in the above proportions, prepared by the proposed method allows to solve the task.

1. Renganathan�age mass of color cordials vessels for anatomical study, containing a mixture of barium sulfate and glycerin, characterized in that a mixture of acrylic paint and an aqueous solution of gelatin.

2. Method of preparing colored radiopaque mass cordials vessels for anatomical study, characterized by the fact that in the mixture of barium sulfate and glycerol was added acrylic paint and gelatin, wherein barium sulfate take 1.5 parts of glycerol, 1 part acrylic paint and 0.1 parts of an aqueous solution of gelatin is added 7.5 parts, stirred, and before applying the mixture is heated to a temperature of 60-80°C.



 

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