Method for producing soluble concentrate from sideline products of reindeer antlers
SUBSTANCE: invention represents the method to obtain the soluble concentrate from sideline products of reindeer antlers, comprising aqueous raw extraction, grounded up to the forced meat condition with particle size of 3-5 mm under the action of ultrasonic vibrations with frequency of 37 kHz with subsequent filtration and vacuum drying at temperature of 45°C and pressure of 0.9 atm, distinguished by the fact that the aqueous raw extraction is carried out at temperature of 35-36°C in the presence of pepsin ferment at its concentration in raw mixture: water of 0.5% during at least 3 hours, at ratio raw: water for tails 1:5, for male genital 1:4, for uteruses with embryos and amniotic fluid 1:2.
EFFECT: significant increase of final concentrate yield from sideline products of reindeer antlers.
3 ex, 2 tbl
The invention relates to cosmetic industry, namely to technology of processing of products of reindeer antlers for the biologically active soluble concentrate in powder form, used for the production of cosmetics.
A method of producing biologically active concentrate of canned antlers, characterized by the fact that the antlers are ground to a particle size of 100 μm and subjected to enzymatic hydrolysis with subsequent extraction, enzymatic hydrolysis was carried out in two stages, the first stage in the presence of the enzyme pepsin at the rate of 2% by weight of antlers when the ratio antlers:water 1:3 for 4 hours and a temperature of 40°C, followed by centrifugation and filtration of the hydrolysate, and the second stage is carried out enzymatic hydrolysis of bagasse obtained after the first stage of fermentation, in the presence of the enzyme papain activity 30 thousand UNITS at the rate of 2% of the initial weight of antlers when the ratio cake:water of 1:1.5 for 4 hours at 40°C, subjected to extraction of the press cake obtained after centrifugation and filtration of the hydrolysate after the second stage of fermentation when the ratio cake:water 1:5 for 3 hours and a temperature of 95-98°C, and drying of all effluents is carried out at a temperature not exceeding 50°C and a vacuum of 0.9 bar (See patent RU 2461384, A61K 35/32, B01D 11/02).
A method of producing a wound-healing drug for external use, based on the preparation of the concentrate from raw deer antler with the addition of form-building substances and preservative. As raw material for the preparation of the concentrate used the uterus of pregnant females with embryo at the age of 2-3 months and amniotic fluid. The concentrate thus prepared by aqueous extraction of shredded to a particle size of 3-5 mm of the uterus with the embryo in a mixture of amniotic fluid with a weight ratio of raw materials:water 1:1 and a temperature of 97-99°C for 4-5 hours followed by filtration and vacuum drying of the extract at a temperature of 55°C and a pressure of 0.9 ATM (Cm. patent RU 2442594 C1, IPC A61K 35/50, A61K 35/12, A61K 35/48, A61K 47/44, A61K 47/46, A61K 9/06, A61P 17/02).
However, this method involves high-temperature extraction of raw materials in native (unmodified) condition, which can lead to coagulation of proteins and as a consequence to the decrease of solubility of the concentrate, and this, in turn, in the manufacture of cosmetics reduces the degree of its impact.
A method of producing a soluble concentrate of by-products of reindeer antlers, comprising an aqueous extraction of raw materials (crushed to a fine mince particle size of 3-5 mm) under the action of ultrasonic Coleman�th a frequency of 37 kHz for 2-3 hours at a ratio of raw materials:water for tailings and/or genital organs of male deer 1:3, for ewes with fruits and amniotic fluid 1:1 at a temperature of 45-50°C followed by filtration and vacuum drying the obtained filtrate at a temperature not exceeding 50°C and a pressure of 0.9 ATM (Cm. patent RU 2488401, IPC A61K 35/12, A61K 35/48, A61K 35/50, B01D 11/02, B01J 19/10 - prototype).
This method ensures that the finished concentrate from tailings at the level of 26.0%, from the genital organs of males is 4.0% of females with embryos of 5.5%.
However, potential biologically active by-products of reindeer antlers not limited to the above percentages of extraction of raw materials of biologically active substances.
Necessary to develop a method of producing a soluble concentrate of by-products of reindeer antlers using tails and genitals of males and females with embryos and amniotic fluid, providing a higher degree of extraction of biologically active substances with high solubility of the finished concentrate.
Said technical result is achieved in that in the method of producing a soluble concentrate of by-products of reindeer antlers, comprising an aqueous extraction of raw materials, ground to a state of minced meat particle size of 3-5 mm under the action of ultrasonic vibrations of a frequency of 37 kHz followed by filtration and vacuum drying at a temperature of 45°C and a pressure of 0.9 ATM, according to W�C to the invention, aqueous extraction of raw materials is carried out at a temperature of 35-36°C in the presence of the enzyme pepsin when its concentration in the mixture of raw materials:water of 0.5% for at least 3 hours when the ratio of raw materials:water tails for 1:5 for genital mutilation of males is 1:4, for dams with fetuses and amniotic fluid 1:2.
The essence of the claimed method is that optimization of the method of obtaining biologically active concentrate is carried out by the intensification of the extraction process, by means of a simultaneous effect on raw ultrasound and enzyme pepsin. Mechanical destruction of particles and intensive mixing of the mixture of raw materials:water when exposed to ultrasound determines greater access pepsin to extractable mass, and found individual the ratio of raw materials:water for every kinds of raw materials allows to intensify the process of ultrasound exposure in each case. This need of search is individual for each type of raw material to the dilution was based on the fact that the intensity of ultrasound exposure to the environment depends on its density and viscosity, that is, different levels of ultrasound exposure to different kinds of raw materials likely stemmed from the specific physico-chemical properties of raw materials, textile structures, etc., leading to different degree of dilution. Cu�IU, in the course of the experiment in connection with the heating of the processed raw material (under the action of ultrasound) emerged the need to reduce maintenance of the temperature of the extraction process to 35-36°C to avoid changes in temperature above 40°C, which is inactivated by the action of pepsin. In the case of increasing the temperature above +40°C-+50°C the enzyme molecule, which is a protein that undergoes a process called denaturing, and the rate of chemical reaction decreases significantly. The optimal concentration of pepsin 0.5% by weight of the mixture of raw materials:water was found in preliminary experiments at the involved concentrations from 0.25 to 1.0%.
Studies have shown when combined in the extraction process of the enzyme pepsin (when its concentration in the mixture of raw materials:water, 0.5%) and ultrasound, the yield of concentrate was increased with the dilution (the ratio of raw material:water) for all types of raw materials (probably due to the dependence of the intensity of ultrasound exposure on the density and viscosity of the medium). So the output of concentrate from tailings at a ratio of raw materials:water 1:2 (when 2-hour extraction) and 19.4%, while the ratio of 1:5 and 32.6% (at 3-hour extraction). A similar trend was observed in the processing of the genital organs of males (11.3 per cent at a ratio of 1:2 and 23.8% at 1:4). For females with embryos and eye�oplatnoy liquid optimal ratio of raw material:water is 1:2, since a further increase in the dilution almost no effect on the output of the concentrate. It should also be noted that the optimum extraction time for all kinds of raw materials amounted to not less than 3-hours, as a 2 hour extraction provided a significantly low level of concentrate output, and when the 4-hour extraction concentrate output increased slightly. In addition, this experiment showed that for high yield of concentrate was important to introduce the enzyme pepsin is not based on his amount of raw material in a mixture of raw materials:water, and its concentration in the mixture. So if you enter 2,0% of the enzyme in the mixture at a ratio of 1:1, compared with a dilution of 1:3, its concentration will be in 2 times less, which significantly influenced the process of entering concentrate. Testing of the claimed method showed that the yield of the finished product in the processing of tails and genitals of males, on average, in comparison with the prototype increased by 1.8 times, and of the uterus with embryos and amniotic fluid - 14,0%.
The study of the biochemical composition of the concentrates obtained by the claimed method and the prototype presented in table 2.
According to table 2 fermentation of raw materials by the enzyme pepsin in the field of ultrasound is possible to increase the content of amino acids 30.1-33.2 per cent, including the essential amino acid 18,0-28,8%, interchangeable - 31.5 to 41.0%. While the content of fat, ash, fatty acids, vitamins and minerals grew by only 6.5 to 12.5 percent. This is because the primary role of pepsin mainly lies in the breakdown of proteins to polypeptides and amino acids.
Example 1. One kilogram tailings were crushed in a cutter or grinder to a particle size of 3-5 mm. Further to 1.0 kg of the crushed tailings were added to 5.0 l of water and 30.0 g of the enzyme pepsin (0.5% pepsin at 6000,0 g of mixture). Ultrasonic extraction at a frequency of 37 kHz (Elmasonic S 80 M) was performed for 3 hours while maintaining a temperature of 35-36°C, at the end of the process, the hydrolysate was filtered and dried at a temperature of 45°C and a pressure of 0.9 ATM.
Example 2. One kilogram of the genital organs of males was ground on the cutter or grinder to a particle size of 3-5 mm. To 1.0 kg of the crushed material was added to 4.0 l of water and 25.0 g of the enzyme pepsin (0.5% pepsin at 5000,0 g of a mixture), then, as in example 1.
Example 3. One kilogram of females with embryos and amniotic fluid were crushed in a cutter or grinder to particles of 3-5 mm. To 1.0 kg of the crushed material was added 2.0 liters of water and 15.0 g of the enzyme pepsin (0.5% pepsin at 3000,0 g of a mixture), then, as in example 1.
The ready solubility of the concentrate by the inventive method exceeded the solubility product prototype slightly (on average by 1.2%).
Thus�time the use of the claimed method of obtaining biologically active concentrate of by-products of reindeer antlers provides a high degree of extraction of biologically active substances at a high percentage of solubility of the finished product.
A method of producing a soluble concentrate of by-products of reindeer antlers, comprising an aqueous extraction of raw materials, ground to a state of minced meat particle size of 3-5 mm under the action of ultrasonic vibrations of a frequency of 37 kHz followed by filtration and vacuum drying at a temperature of 45°C and a pressure of 0.9 ATM, characterized in that the aqueous extraction of raw materials is carried out at a temperature of 35-36°C in the presence of the enzyme pepsin when its concentration in the mixture of raw materials:water of 0.5% for at least 3 hours, at a ratio of raw material:water tails 1:5, for the genital organs of males 1:4, for dams with fetuses and amniotic fluid 1:2.
SUBSTANCE: method includes placing liquid media and objects located in a medium inside a mechanical oscillatory channel system, having a natural oscillation frequency, in which parametric resonances are excited or parametric excitation of self-oscillation is performed, setting as the cavitational processing efficiency criterion the oscillation amplitude of the channel system, determining optimum frequency of oscillation frequency of power exciters with experimentally predetermined natural and parametric oscillation frequencies.
EFFECT: shorter processing time, higher power of acoustic waves and higher efficiency of cavitational processing of liquid media and objects located in the media.
FIELD: technological processes.
SUBSTANCE: invention relates to devices for physical and chemical treatment by ultrasonic cavitation of molecular and colloid solutions, and also disperse systems, phases of which may include live forms, by initiation of sonochemical reactions in media of such solutions and cavitation erosion of their phases. A sonochemical reactor for treatment of liquids includes a working volume filled with liquid flowing through it and limited with the surface of the body, which belong to a single solid-state vibrating system, from metal by two emitting surfaces and a binding surface between them, at the same time the centre of mass of this system is the centre of its geometric symmetry, for which purpose emitting surfaces belong to acoustic waveguide transformers of identical shape, dimensions and mass, transmitting coherent cophased flat-elastic oscillations from both sides from sources into liquid at frequency of free elastic oscillations of this solid-state vibrating system from metal, into composition of which they are included, and binding length makes half of length of vibration wave in metal, from which it is made.
EFFECT: invention provides for increased acoustic capacity of a cavitation reactor and increased efficiency of liquid treatment in it due to reduced losses of energy of ultrasound exciting cavitation.
2 cl, 4 dwg, 1 tbl
SUBSTANCE: invention relates to field of chemical technology of energy-saturated materials, namely to methods of utilisation of formed wastes of production of defective and expired products, and is intended for laboratory methods of trinitrotoluene decomposition. Method of trinitrotoluene destruction consists in influencing trinitrotoluene in water medium with alkaline chemical reagents - water solutions of sodium sulphite and sodium hydroxide with concentration 5-20%, with weight ratio trinitrotoluene: chemical reagent, equal 1:3-30, and simultaneous impact with ultrasonic fluctuations with frequency not lower than 20 kHz and intensity not lower than 2,5 W/cm2; and at initial temperature 40-50°C heating is realised due to absorption of energy of ultrasonic fluctuations to temperature 80-85°C.
EFFECT: invention provides complete decomposition of explosive substance, absence of toxic organic products and high rate of the process.
9 dwg, 1 tbl
FIELD: power industry.
SUBSTANCE: heat-mass-energy exchange method consists in formation of vortex annular flows of media, their direction parallel to each other with provision of partial contact of opposite directed external surface layers in radial and tangential directions to the depth providing their acoustical excitation due to deformation-shear interaction, and further combination of excited flows. One of the vortex annular flows is additionally directed in an opposite direction to the rest ones along their axes. A heat-mass-energy exchange device containing two pipes with tangential inlets, which are interconnected with each other by partial intersection along generatrixes, and an acoustical chamber; with that, tangential inlets and the outlet of one of the pipes are located in an opposite direction in relation to the tangential inlets and outlets of the rest pipes.
EFFECT: invention provides for additional excitation of flows in the zone of their contact and increase of heat-mass-energy exchange intensity.
2 cl, 6 dwg
SUBSTANCE: invention relates to obtaining biocompatible magnetic nano-particles and can be applied for therapeutic purposes, in particular, for fighting cancer. Method of obtaining nano-particles, including iron oxide and silicon-containing casing and having value of specific absorption rate (SAR) 10-40 W per g of Fe with field strength 4 kA/m and frequency of alternating magnetic field 100 kHz, contains the following stages: A1) preparation of composition of at least one iron-containing compound in at least one organic solvent; B1) heating of composition to temperature in range from 50°C to temperature 50°C lower than temperature of reaction of iron-containing compound according to stage C1 for minimal period 10 minutes; C1) heating composition to temperature between 200°C and 400°C; D1) purification of obtained particles; E1) suspending purified nano-particles in water or water acid solution; F1) addition of surface-active compound to water solution, obtained according to stage E1); G1) processing of water solution according to stage F1) by ultrasound; H1) purification of water dispersion of particles, obtained according to stage G1); I1) obtaining dispersion of particles according to stage H1) in mixture of solvent from water and water-mixable solvent; J1) addition of alkoxysilane into dispersion of particles in mixture of solvent according to stage I1); and K1) purification of particles.
EFFECT: invention makes it possible to obtain biocompatible magnetic particles with high value of specific absorption rate (SAR).
42 cl, 3 dwg, 9 ex
SUBSTANCE: invention refers to medicine, namely to a method for preparing a soluble concentrate of deer-breeding antler by-products. The method for preparing the soluble concentrate of deer-breeding antler by-products involving water extraction of raw materials when exposed to ultrasonic vibrations at a ratio of raw materials: water 1:3 for male deer tails and/or reproductive organs, 1:1 for uterus with foetuses and amniotic fluid; that is followed by filtration and vacuum drying, under certain conditions.
EFFECT: method provides higher yield of the end product and keeping the natural properties of the raw material and a high degree of water-solubility.
3 tbl, 3 ex
SUBSTANCE: invention relates to the technology of processing mineral material and can be used to produce amorphous silicon dioxide from rice husks. The method producing amorphous silicon dioxide from rice husks involves washing rice husks with deionised water in an ultrasonic field in cavitation mode while heating to 90°C, for 10 minutes at frequency of 20 kHz and 20 minutes at frequency of 35-60 kHz, respectively. Carbonisation is then carried out, as well as grinding the ash and oxidising roasting in a reactor lined with quartz glass, while constantly stirring in a current of cleaned air and raising temperature at not more than 10°C/min.
EFFECT: invention enables to obtain, using an ecologically clean method, amorphous silicon dioxide with purity of up to 99,99%, specific surface area of up to 420 m2/g and particle size of 10-80 nm.
3 cl, 5 ex
FIELD: process engineering.
SUBSTANCE: invention relates to cavitation treatment of fluid media and those wherein specific content of water or other fluid medium exceed 65-70% of total amount. Proposed method consists in placing whatever volumes in channel with fluid wherein flat standing acoustic wave is generated to pass through processed volumes, primarily, perpendicular to their position. Material of said volumes features specific acoustic resistance equal to or approximating to resistance of fluid filling channel or to that of processed fluid. Amplitude of ultrasound resonance oscillations exceeds the threshold of acoustic cavitation for fluids processed at the given moment with due allowance for losses wall penetration losses. Fluid processing temperature is set by channel fluid. Width between channel walls is multiple of the quarter of channel fluid acoustic wavelength.
EFFECT: ultrasound processing at required temperature conditions.
FIELD: process engineering.
SUBSTANCE: invention relates to cavitation treatment of fluid media and those therein specific content of water or other fluid medium exceed 65-70% of total amount. Proposed method consists in generating acoustic cavitation simultaneously in two or several different frequencies. Note here that mechanical oscillating system composed of rectangular cross-section channel consisting of sequential membranes with different frequencies of fundamental harmonic of oscillations. Standing wave sound oscillations are generated in-phase at channel opposite sides to generate quasi flat standing waves between channel walls corresponding to frequencies of membrane oscillations. Channel gap width is selected to make multiple of the wavelength quarter excited in given fluid for used frequencies. Channel wall oscillation amplitude is selected to be optimum for different fluid treatment steps to exceed acoustic cavitation threshold.
EFFECT: higher efficiency at reduced power output of ultrasound radiators.
SUBSTANCE: invention relates to hydrodynamics and a method of exciting acoustic vibrations in a fluid medium and an apparatus for realising the method. The method involves forming a stream of the fluid medium and feeding said stream into a treatment zone having reflectors and flexible obstacles in form of mechanical resonators, generating parametric resonance vibrations at each point of the fluid medium, simultaneously increasing the amplitude of vibrations of each flexible obstacle in resonance conditions with parametric resonance vibrations, exciting harmonic acoustic vibrations in the fluid medium with formation of a cavitation zone and removing the treated fluid medium from the treatment zone. Vibrations are excited by moving the fluid medium relative the flexible obstacles which are fitted with cavitators which are mounted on mechanical resonators or are made in form of ends of resonators which cambered towards the incident flow, wherein the fluid medium flows around the obstacles in developed cavitation flow conditions with formation of a pulsating cavitation zone (cavern) whose surface serves as a source of acoustic vibrations, and resonance conditions of a defined overtone are created by placing flexible obstacles relative reflectors and each other at a distance which depends on acoustic properties of the fluid medium. The apparatus according to one of the versions has a housing made from at least two flat or curved plates which act as reflectors; mechanical resonators are made with ends which are cambered towards the incident flow or are fitted with cavitators, wherein the distance from the reflection points lying on the reflectors to mechanical resonators and the distance between the mechanical resonators is a multiple of λ/4, where λ is the wavelength of the fluid medium which corresponds to the frequency of acoustic vibrations in a defined medium.
EFFECT: invention increases efficiency of exciting and generating vibrations of a fluid medium, widens the cavitation zone and also enables to obtain abrasive media which are stable over time, such media including dispersions, suspensions and emulsions, suitable for prolonged storage without deterioration of properties obtained from treatment owing to use of a fluid medium as an acoustic vibration radiator.
9 cl, 3 dwg
SUBSTANCE: invention relates to method of simultaneous obtaining of two flavonoids - patuletine and its 7-O-β-D-glucopyranoside - patuletrine. Method consists in the following: milled edge petals of flower of high flavonoid sorts of tagetes patula are extracted with hexane, dried and r-extracted with chloroform, chloroform extract is concentrated, dry residue is dissolved in mixture of petroleum ether - chloroform, precipitated sediment is filtered, washed with petroleum ether and dried, obtained dry powder is dissolved in mixture chloroform - ethanol, precipitated sediment is filtered, washed with petroleum ether and dried with obtaining patuletine. Then, extraction of raw material, which remains after chloroform processing, is carried out with ethanol, alcohol extract is filtered and concentrated, after that, water residue is subjected to liquid phase extraction with ethylacetate, then, organic layer is concentrated, dry residue is dissolved in mixture chloroform - ethylacetate, precipitated sediment is filtered, washed with cooled ethylacetate, solution of hydrochloric acid in ethanol, ethanol and ethylacetate and dried, dry powder is dissolved in mixture ethylacetate-ethanol, precipitated sediment is filtered, washed with ethylacetate and dried with obtaining patuletrine.
EFFECT: method makes it possible to obtain highly pure samples of patuletine and patuletrine, as well as increase target product output.
4 dwg, 3 tbl, 4 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to pharmaceutical industry, namely to a method for preparing lappaconitine hydrobromide (versions) A method for preparing lappaconitine hydrobromide is implemented by extraction of Aconitum leucostomum root and herb or Aconitum septentrionale root and herb in methylene chloride in a continuous extraction apparatus that is followed by decontamination by means of flash chromatography (version 1), or extraction of a herbal raw material in a polar organic solvent followed by extract removal from the organic solvent (version 2), alkalinisation and extraction of the prepared extract in methylene chloride followed by decontamination of the extract by flash chromatography.
EFFECT: method for preparing lappaconitine hydrobromide provides simplifying the technological process, reducing its length and improving higher yield of the end product of officinal purity.
7 cl, 1 tbl, 9 ex
FIELD: food industry.
SUBSTANCE: method for production of Siberian cedar seeds liqueur (with hepatoprotective, antioxidant, antihypoxic, hypolipidemic effect) by way of maceration with ethyl alcohol usage; whole Siberian cedar seeds are loaded into the reactor, poured with 70% ethyl alcohol water solution; extraction is performed under preset conditions. The medicinal preparation with hepatoprotective, antioxidant, antihypoxic, hypolipidemic effect contains Siberian cedar seeds liqueur. Usage of the medicinal preparation as a hepatoprotective remedy.
EFFECT: liqueur has pronounced hepatoprotective, antioxidant, antihypoxic and hypolipidemic effect.
6 cl, 3 dwg, 8 tbl
SUBSTANCE: method for producing a pigment complex of bisnaphthazarin for preventing inflammatory diseases, involving demineralising commercial sea urchins' crusts and needles in an organic acid solution, separating organic acid salts and protein, applying pigment solution on a chromatographic column, washing the column with diluted mineral acid and distilled water, eluting the pigment complex, combining fractions containing the pigments, removing ethanol, lyophilising concentrate in the certain environment. The complex of pigments bisnaphthazarins for preventing inflammatory diseases.
EFFECT: complex of pigments prepared by the above method is effective for preventing the inflammatory diseases.
3 cl, 2 dwg, 2 tbl, 4 ex
FIELD: machine building.
SUBSTANCE: for extraction (leaching) of the substances extracted from the plant materials in food, chemical-pharmaceutical and other industries, for output increasing of the substances extracted from the extractable plant materials and for increasing of their concentration in the ready extraction it is suggested to provide the extractor with the extractant recirculation circuit containing devices for solid phase separation, pump, discharge tank, flowmeter, shutdown valves system, and in the extractor bottom part additionally union will be installed for continuous liquid phase supply.
EFFECT: wider possibility of return in the vessel of part of extraction after solid phase separation in specified ratio with fresh extractant, thus improving conditions of mass exchange due to decreasing of the surface tension of the liquid phase.
SUBSTANCE: method for preparing an agent possessing anti-inflammatory, diuretic and antioxidant activity, involving milling Spiraea salicifolia shoots representing a mixture of leaves, blossom and shoots, extracting them three times by gradual maceration, mixing in infusing, filtering, condensing, separating, drying in the certain environment.
EFFECT: agent shoes the pronounced anti-inflammatory, diuretic and antioxidant activity.
2 dwg, 12 tbl
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to pharmaceutical and consumer industry in preparing dry herbal extracts used for later colouration of textile fabrics with this extract. A method for preparing the dry herbal extract of St. John's wort involves grinding herbal raw material, extracting in water, filtering, boiling down in a rotary evaporator and drying the residual product to constant weight in a drying chamber in the certain environment.
EFFECT: method described above enables preparing the extract with dry colorant weight 25% of dry herbal raw material weight.
SUBSTANCE: method of obtaining sanguinarine and chelerythrine sulphates includes extraction of milled overground part of macleaya microcarpa and/or macleaya cordata with water aliphatic alcohol, removal of water aliphatic alcohol in vacuum, alkalinisation of water distillation residue with hydrophobic solvent, processing organic phase with sulphuric acid, filtration, washing and drying of target product, with extraction of milled raw material being carried out with water aliphatic alcohol in presence of methanesulphoacid, and solution of alkaloid bases in hydrophobic organic solvent is additionally filtered through layer of hydrophobic solvent, and target product is subjected to boiling in acetone.
EFFECT: method makes it possible to increase quality of finished product, simplify technology of production of sanguinarine and chelerythrine sulphates, and reduce the process duration.
8 cl, 14 ex
SUBSTANCE: method of bee pollen processing consists in the fact, that bee pollen is extracted with CO2 by pumping CO2, obtained fat extract is separated, remaining oil-seed meal is subjected to enzymatic hydrolysis in presence of enzyme Distizym Protacide Extra, obtained fermentolisate is separated into solid and liquid phases, solid phase is dried, liquid phase is filtered, preservative potassium sorbate and sodium benzoate are added into filtered liquid fraction under specified conditions.
EFFECT: method makes it possible to efficiently process bee pollen with obtaining fractions, characterised by microbiological purity, high degree of bioavailability, and a long storage term.
SUBSTANCE: invention relates to versions of composition for heat transmission. One of composition versions contains (i) from about 20 to about 90 wt % of R-1234yf; (ii) from about 10 to about 60 wt % of R-134a and (iii) from about 1 to about 20 wt % of R-32. Invention also relates to a number of versions of composition application.
EFFECT: composition has lower value of global warming potential and at the same time is characterised by productivity and energy efficiency.
56 cl, 7 dwg
SUBSTANCE: invention refers to medicine, particularly to a method for active substance (AS) delivery through an epidermal barrier. The declared method involves using a matrix-type transdermal patch containing a substrate, a protective tape and a polymer layer, and characterised by the fact that 10% PEG-12 dimethicone niosomes are introduced into the polymer layer of the transdermal patch; then the substrate is coated with the polymer layer. The ACs are encapsulated into the niosomes with the use of hemogeniser APV, as well as 10 wt % propylene glycol and 5 wt % isopropyl myristate.
EFFECT: improving the active substance penetration with maintaining the biological activity and prolonging the active substance action.
2 tbl, 3 ex