Method for producing soluble concentrate from sideline products of reindeer antlers

FIELD: aviation.

SUBSTANCE: invention represents the method to obtain the soluble concentrate from sideline products of reindeer antlers, comprising aqueous raw extraction, grounded up to the forced meat condition with particle size of 3-5 mm under the action of ultrasonic vibrations with frequency of 37 kHz with subsequent filtration and vacuum drying at temperature of 45°C and pressure of 0.9 atm, distinguished by the fact that the aqueous raw extraction is carried out at temperature of 35-36°C in the presence of pepsin ferment at its concentration in raw mixture: water of 0.5% during at least 3 hours, at ratio raw: water for tails 1:5, for male genital 1:4, for uteruses with embryos and amniotic fluid 1:2.

EFFECT: significant increase of final concentrate yield from sideline products of reindeer antlers.

3 ex, 2 tbl

 

The invention relates to cosmetic industry, namely to technology of processing of products of reindeer antlers for the biologically active soluble concentrate in powder form, used for the production of cosmetics.

A method of producing biologically active concentrate of canned antlers, characterized by the fact that the antlers are ground to a particle size of 100 μm and subjected to enzymatic hydrolysis with subsequent extraction, enzymatic hydrolysis was carried out in two stages, the first stage in the presence of the enzyme pepsin at the rate of 2% by weight of antlers when the ratio antlers:water 1:3 for 4 hours and a temperature of 40°C, followed by centrifugation and filtration of the hydrolysate, and the second stage is carried out enzymatic hydrolysis of bagasse obtained after the first stage of fermentation, in the presence of the enzyme papain activity 30 thousand UNITS at the rate of 2% of the initial weight of antlers when the ratio cake:water of 1:1.5 for 4 hours at 40°C, subjected to extraction of the press cake obtained after centrifugation and filtration of the hydrolysate after the second stage of fermentation when the ratio cake:water 1:5 for 3 hours and a temperature of 95-98°C, and drying of all effluents is carried out at a temperature not exceeding 50°C and a vacuum of 0.9 bar (See patent RU 2461384, A61K 35/32, B01D 11/02).

A method of producing a wound-healing drug for external use, based on the preparation of the concentrate from raw deer antler with the addition of form-building substances and preservative. As raw material for the preparation of the concentrate used the uterus of pregnant females with embryo at the age of 2-3 months and amniotic fluid. The concentrate thus prepared by aqueous extraction of shredded to a particle size of 3-5 mm of the uterus with the embryo in a mixture of amniotic fluid with a weight ratio of raw materials:water 1:1 and a temperature of 97-99°C for 4-5 hours followed by filtration and vacuum drying of the extract at a temperature of 55°C and a pressure of 0.9 ATM (Cm. patent RU 2442594 C1, IPC A61K 35/50, A61K 35/12, A61K 35/48, A61K 47/44, A61K 47/46, A61K 9/06, A61P 17/02).

However, this method involves high-temperature extraction of raw materials in native (unmodified) condition, which can lead to coagulation of proteins and as a consequence to the decrease of solubility of the concentrate, and this, in turn, in the manufacture of cosmetics reduces the degree of its impact.

A method of producing a soluble concentrate of by-products of reindeer antlers, comprising an aqueous extraction of raw materials (crushed to a fine mince particle size of 3-5 mm) under the action of ultrasonic Coleman�th a frequency of 37 kHz for 2-3 hours at a ratio of raw materials:water for tailings and/or genital organs of male deer 1:3, for ewes with fruits and amniotic fluid 1:1 at a temperature of 45-50°C followed by filtration and vacuum drying the obtained filtrate at a temperature not exceeding 50°C and a pressure of 0.9 ATM (Cm. patent RU 2488401, IPC A61K 35/12, A61K 35/48, A61K 35/50, B01D 11/02, B01J 19/10 - prototype).

This method ensures that the finished concentrate from tailings at the level of 26.0%, from the genital organs of males is 4.0% of females with embryos of 5.5%.

However, potential biologically active by-products of reindeer antlers not limited to the above percentages of extraction of raw materials of biologically active substances.

Necessary to develop a method of producing a soluble concentrate of by-products of reindeer antlers using tails and genitals of males and females with embryos and amniotic fluid, providing a higher degree of extraction of biologically active substances with high solubility of the finished concentrate.

Said technical result is achieved in that in the method of producing a soluble concentrate of by-products of reindeer antlers, comprising an aqueous extraction of raw materials, ground to a state of minced meat particle size of 3-5 mm under the action of ultrasonic vibrations of a frequency of 37 kHz followed by filtration and vacuum drying at a temperature of 45°C and a pressure of 0.9 ATM, according to W�C to the invention, aqueous extraction of raw materials is carried out at a temperature of 35-36°C in the presence of the enzyme pepsin when its concentration in the mixture of raw materials:water of 0.5% for at least 3 hours when the ratio of raw materials:water tails for 1:5 for genital mutilation of males is 1:4, for dams with fetuses and amniotic fluid 1:2.

The essence of the claimed method is that optimization of the method of obtaining biologically active concentrate is carried out by the intensification of the extraction process, by means of a simultaneous effect on raw ultrasound and enzyme pepsin. Mechanical destruction of particles and intensive mixing of the mixture of raw materials:water when exposed to ultrasound determines greater access pepsin to extractable mass, and found individual the ratio of raw materials:water for every kinds of raw materials allows to intensify the process of ultrasound exposure in each case. This need of search is individual for each type of raw material to the dilution was based on the fact that the intensity of ultrasound exposure to the environment depends on its density and viscosity, that is, different levels of ultrasound exposure to different kinds of raw materials likely stemmed from the specific physico-chemical properties of raw materials, textile structures, etc., leading to different degree of dilution. Cu�IU, in the course of the experiment in connection with the heating of the processed raw material (under the action of ultrasound) emerged the need to reduce maintenance of the temperature of the extraction process to 35-36°C to avoid changes in temperature above 40°C, which is inactivated by the action of pepsin. In the case of increasing the temperature above +40°C-+50°C the enzyme molecule, which is a protein that undergoes a process called denaturing, and the rate of chemical reaction decreases significantly. The optimal concentration of pepsin 0.5% by weight of the mixture of raw materials:water was found in preliminary experiments at the involved concentrations from 0.25 to 1.0%.

Studies have shown when combined in the extraction process of the enzyme pepsin (when its concentration in the mixture of raw materials:water, 0.5%) and ultrasound, the yield of concentrate was increased with the dilution (the ratio of raw material:water) for all types of raw materials (probably due to the dependence of the intensity of ultrasound exposure on the density and viscosity of the medium). So the output of concentrate from tailings at a ratio of raw materials:water 1:2 (when 2-hour extraction) and 19.4%, while the ratio of 1:5 and 32.6% (at 3-hour extraction). A similar trend was observed in the processing of the genital organs of males (11.3 per cent at a ratio of 1:2 and 23.8% at 1:4). For females with embryos and eye�oplatnoy liquid optimal ratio of raw material:water is 1:2, since a further increase in the dilution almost no effect on the output of the concentrate. It should also be noted that the optimum extraction time for all kinds of raw materials amounted to not less than 3-hours, as a 2 hour extraction provided a significantly low level of concentrate output, and when the 4-hour extraction concentrate output increased slightly. In addition, this experiment showed that for high yield of concentrate was important to introduce the enzyme pepsin is not based on his amount of raw material in a mixture of raw materials:water, and its concentration in the mixture. So if you enter 2,0% of the enzyme in the mixture at a ratio of 1:1, compared with a dilution of 1:3, its concentration will be in 2 times less, which significantly influenced the process of entering concentrate. Testing of the claimed method showed that the yield of the finished product in the processing of tails and genitals of males, on average, in comparison with the prototype increased by 1.8 times, and of the uterus with embryos and amniotic fluid - 14,0%.

The study of the biochemical composition of the concentrates obtained by the claimed method and the prototype presented in table 2.

According to table 2 fermentation of raw materials by the enzyme pepsin in the field of ultrasound is possible to increase the content of amino acids 30.1-33.2 per cent, including the essential amino acid 18,0-28,8%, interchangeable - 31.5 to 41.0%. While the content of fat, ash, fatty acids, vitamins and minerals grew by only 6.5 to 12.5 percent. This is because the primary role of pepsin mainly lies in the breakdown of proteins to polypeptides and amino acids.

Example 1. One kilogram tailings were crushed in a cutter or grinder to a particle size of 3-5 mm. Further to 1.0 kg of the crushed tailings were added to 5.0 l of water and 30.0 g of the enzyme pepsin (0.5% pepsin at 6000,0 g of mixture). Ultrasonic extraction at a frequency of 37 kHz (Elmasonic S 80 M) was performed for 3 hours while maintaining a temperature of 35-36°C, at the end of the process, the hydrolysate was filtered and dried at a temperature of 45°C and a pressure of 0.9 ATM.

Example 2. One kilogram of the genital organs of males was ground on the cutter or grinder to a particle size of 3-5 mm. To 1.0 kg of the crushed material was added to 4.0 l of water and 25.0 g of the enzyme pepsin (0.5% pepsin at 5000,0 g of a mixture), then, as in example 1.

Example 3. One kilogram of females with embryos and amniotic fluid were crushed in a cutter or grinder to particles of 3-5 mm. To 1.0 kg of the crushed material was added 2.0 liters of water and 15.0 g of the enzyme pepsin (0.5% pepsin at 3000,0 g of a mixture), then, as in example 1.

The ready solubility of the concentrate by the inventive method exceeded the solubility product prototype slightly (on average by 1.2%).

Thus�time the use of the claimed method of obtaining biologically active concentrate of by-products of reindeer antlers provides a high degree of extraction of biologically active substances at a high percentage of solubility of the finished product.

A method of producing a soluble concentrate of by-products of reindeer antlers, comprising an aqueous extraction of raw materials, ground to a state of minced meat particle size of 3-5 mm under the action of ultrasonic vibrations of a frequency of 37 kHz followed by filtration and vacuum drying at a temperature of 45°C and a pressure of 0.9 ATM, characterized in that the aqueous extraction of raw materials is carried out at a temperature of 35-36°C in the presence of the enzyme pepsin when its concentration in the mixture of raw materials:water of 0.5% for at least 3 hours, at a ratio of raw material:water tails 1:5, for the genital organs of males 1:4, for dams with fetuses and amniotic fluid 1:2.



 

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