Method of obtaining patuletine and patuletine-7-o-β-d-glucopyranoside (patuletrine) from vegetable raw material

FIELD: chemistry.

SUBSTANCE: invention relates to method of simultaneous obtaining of two flavonoids - patuletine and its 7-O-β-D-glucopyranoside - patuletrine. Method consists in the following: milled edge petals of flower of high flavonoid sorts of tagetes patula are extracted with hexane, dried and r-extracted with chloroform, chloroform extract is concentrated, dry residue is dissolved in mixture of petroleum ether - chloroform, precipitated sediment is filtered, washed with petroleum ether and dried, obtained dry powder is dissolved in mixture chloroform - ethanol, precipitated sediment is filtered, washed with petroleum ether and dried with obtaining patuletine. Then, extraction of raw material, which remains after chloroform processing, is carried out with ethanol, alcohol extract is filtered and concentrated, after that, water residue is subjected to liquid phase extraction with ethylacetate, then, organic layer is concentrated, dry residue is dissolved in mixture chloroform - ethylacetate, precipitated sediment is filtered, washed with cooled ethylacetate, solution of hydrochloric acid in ethanol, ethanol and ethylacetate and dried, dry powder is dissolved in mixture ethylacetate-ethanol, precipitated sediment is filtered, washed with ethylacetate and dried with obtaining patuletrine.

EFFECT: method makes it possible to obtain highly pure samples of patuletine and patuletrine, as well as increase target product output.

4 dwg, 3 tbl, 4 ex

 

The invention relates to the field of Bioorganic chemistry and relates to a method for the simultaneous receipt of two flavonoids - patuletin and its 7-O-β-D-glucopyranoside (patuletin).

The following methods are known simultaneous selection patuletin and patuletin from vegetable raw materials:

- from the flowers of Inula britannica (family Asteraceae) with the use of raw material extraction with 80% methanol, liquid-phase extraction of the methanol extract, hexane, chloroform and butanol, separated by column chromatography butanone fraction on silica gel, MCl gel, Sephadex LH-20 and preparative HPLC; yield patuletin - 8.97·10-4%; patuletin - 1.15-10-4% [1];

- from herb Tagetes mendocina (family Asteraceae) with the use of raw material extraction with hexane, dichloromethane and methanol, liquid-phase extraction of methanol extracts of diethyl ether and ethyl acetate, separated by column chromatography of the ether fraction on Sephadex LH-20 and preparative HPLC; yield patuletin - 1.34·10-2%; separated by column chromatography water residue on a Sephadex LH-20; output patuletin - 5.40·10-3% [2];

- from flowers of Tagetes patula (family Asteraceae) with the use of raw material extraction with 80% ethanol, preparative HPLC alcoholic extract; output patuletin - 0.01%, patuletin - 0.01% [3];

- from flowers of Tagetes patula (family Asteraceae) with the use of raw material extraction with methanol, liquid-phase extraction �etnologi extract petroleum ether and chloroform, stepwise extraction of the aqueous residue with ethyl acetate followed by concentration and pooling of fractions of the same composition; the output patuletin - 0.55%, patuletin - 0.11% [4].

The disadvantages of the known methods is the use of chromatographic steps, which increases the cost of the target products, as well as the low yield of the target product due to the use of whole herbs or flowers. It has been previously shown that the distribution of flavonoids in the flowers of the marigold open (Tagetes patula) unevenly. So the total content of this class of compounds in the boundary petals is 10-11%, in tubular - 5-6%, in the receptacle is 4-5% [5]. In this regard, as an alternative source for obtaining the dominant flavonoids marigold open - patuletin and patuletin offered marginal petals vysokoplodorodnyh varieties of Tagetes patula.

The object of the invention is to simplify the method of obtaining high-purity samples patuletin and patuletin, as well as increase the yield of target products.

The technical result of the invention (getting patuletin and patuletin) is achieved through the use of boundary petals flowers vysokoplodorodnyh varieties of marigolds open.

To achieve the said technical result of the crushed plant material (marginal petals cvetko� marigold open) extracted in Soxhlet apparatus with hexane in the ratio raw: extractant 1:3, then skim the raw material was extracted with the chloroform in the ratio raw:extractant 1:(3.5-4.5). The organic extract was concentrated in vacuo to a dry residue, which was dissolved in a mixture of petroleum ether - chloroform (1:1) in a ratio of 1:1 when heated to 40°C and leave at 4°C for 36-40 hours. The precipitate was filtered off on a quartz filter, washed with petroleum ether until colorless wash water and dried in vacuo. The yield of the target product patuletin is 3.33-4.10% by weight of vegetable raw materials. Plant material after treatment with chloroform, extracted with 90% ethanol in the ratio raw:extractant 1:(15-20) three times at a temperature of 85-90°C for 60-90 min. ethanol extract was filtered, pooled and concentrated in vacuo to 1/50 of the original volume. The aqueous residue was subjected to liquid-phase extraction by ethyl acetate in the ratio of the residue:ethyl acetate 1:(0.3-0.5). Ethylacetate extraction were pooled, concentrated in vacuo to dryness and dissolved in a mixture of chloroform - ethyl acetate (1:2) in a ratio of 1:1 when heated to 50°C and leave at a temperature of 48°C for 36-40 hours. The precipitate was filtered off on a quartz filter, washed with chilled ethyl acetate (1-2°C), 0.1% solution of hydrochloric acid in 96% ethanol, 96% ethanol and ethyl acetate �about colorless wash water and dried in vacuo. The output patuletin is 6.02-7.80% by weight of vegetable raw materials.

Identified features allow to make a conclusion about conformity of the proposed technological solution to the criterion "novelty".

The proposed method allows to obtain substance patuletin and patuletin with the following properties:

- patuletin - amorphous greenish-yellow powder, without taste or smell, insoluble in hexane, petroleum ether and water, sparingly soluble in 30% ethanol and ethyl acetate, soluble in chloroform, methanol and 96% ethanol. The loss in mass on drying - 2-3%;

- patuletin - yellow amorphous powder, without taste or smell, insoluble in hexane, petroleum ether, chloroform and water, moderately soluble in 20% ethanol and ethyl acetate, soluble in 80% methanol and 70% ethanol. Mass loss during drying is 2-3%.

A method of producing patuletin and patuletin is confirmed by the following examples.

Example 1. 250 g of crushed boundary marigold petals open variety "petite orange" is placed in the cartridge from the filter paper, the resulting cartridge is made in an extraction flask Soxhlet extraction. In a flask pour 750 ml of hexane, attached to the extraction flask and return to the fridge and extraction is carried out at 60°C for 36 h. the Cartridge after extraction hexaamminecobalt and repeat the extraction with 875 ml of chloroform in a similar situation. Chloroform extract was concentrated in vacuo at 60°C until complete removal of solvent. The dry residue is dissolved in 120 ml of a mixture of petroleum ether - chloroform (1:1) by heating to 40°C and leave at 4°C for 36-40 hours. The precipitate was filtered off on a quartz filter (pore 160), washed with 150 ml petroleum ether and dried in vacuo. The dry powder was dissolved in 100 ml of a mixture of chloroform - ethanol (5:1) and allowed to stand at 4°C for 24-30 hours. The precipitate was filtered off on a quartz filter (pore 160), washed with 100 ml of petroleum ether and dried in vacuo. Obtained 10.25 g patuletin (4.10% by weight of air-dry raw material).

Cartridge after extraction with chloroform, dried, loose plant material, transfer it into a flask with a ground joint with a capacity of 5 liters, pour 3.75 l of 90% ethanol, attach return the fridge and perform the extraction in a water bath at a temperature of 85-90°C for 90 min. ethanol extract was filtered. Extraction with 90% ethanol is repeated in the same conditions two more times. The combined ethanol extract was concentrated in vacuo to 1.0-1.2 l Water the residue transferred to a separatory funnel with a volume of 2 litres, pour 400 ml of ethyl acetate, stirred for 10 min and left until the bundle. The organic layer is taken; liquid-phase extraction with an aqueous �Loja repeat three more times. The combined organic layer was concentrated in vacuo to remove the solvent. The dry residue was dissolved in 100 ml of a mixture of chloroform - ethyl acetate (1:2) when heated to 50°C and leave at 4°C for 36-40 hours. The precipitate was filtered off on a quartz filter, washed with 50 ml of cooled ethyl acetate (1-2°C), 100 ml of 0.1% hydrochloric acid in 96% ethanol, 200 ml of 96% ethanol and 100 ml of ethyl acetate and dried in vacuo. The dry powder was dissolved in 150 ml of a mixture ethyl acetate - ethanol (3:1) and allowed to stand at 4°C for 24-30 hours. The precipitate was filtered off on a quartz filter (pore 160), washed with 100 ml of ethyl acetate and dried in vacuo. Received 19.49 g patuletin (7.80% by weight of air-dry raw material).

Example 2. The process of allocating patuletin and patuletin carried out analogously to example 1; the difference is that the extraction is carried out from the edge of the petals of the marigold open class "Bolero". The yield of the target product patuletin - 8.32 g (3.33% by weight of air-dry raw material), patuletin - 15.04 g (6.02% by weight of air-dry raw material).

Example 3. The process of allocating patuletin and patuletin carried out analogously to example 1; the difference is that the extraction is carried out from the edge of the petals of the marigold prostrate cultivar "Red cherry". The output patuletin - 9.25 g (3.70% by weight of air-dry raw material), p�toletana -17.33 g (6.93% by weight of air-dry raw material).

Example 4. The process of allocating patuletin and patuletin carried out analogously to example 1; the difference is that the extraction is carried out from the edge of the petals of the marigold prostrate varieties of "dune". The output patuletin - 9.04 g (3.62% by weight of air-dry raw material), patuletin - 16.58 g (6.63% by weight of air-dry raw material).

Physico-chemical properties patuletin and patuletin

Selected compounds according to physico-chemical analysis are 3,5,7,3',4'-tetrahydroxy-6-methoxyflavone (patuletin) and 3,5,7,3',4'-tetrahydroxy-6-methoxyflavone-7-O-β-D-glucopyranosid (patuletin) (Fig.1). The main properties of the compounds are shown below.

Paullin. The UV spectrum (MeOH, λmax, nm): 258, 370. Brutto-formula: C16N12O8. HR-ESI-MC-spectrum (m/z): 355.014 [M+Na]+. EI+-MS spectrum (m/z): 333 [M+H]+.13C-NMR spectrum (125 MHz; δ, M. D.): 61.1 (CH3, 6-och3), 94.7 (CH, C-8), 104.4 (C-10), 116.4 (CH, C-2'), 116.7 (CH, C-5'), 120.8 (CH, C-6'), 121.3 (C, C-10, 132.3 (S, 6), 137.6 (S, 3), 146.2 (S, 30, 148.0 (S, 2), 148.7 (C, C-4'), CHENGDE 151.7 (S, 5), 152.8 (C, C-9), 158.9 (S, 7), 177.2 (S, 4).

Poultry. The UV spectrum (MeOH, λmax, nm): 259, 372. Brutto-formula: C22N22O13. HR-ESI-MC-spectrum (m/z): 517.112 [M+Na]+. EI+-MS spectrum (m/z): 495 [M+H]+, 333 [(M-glucose)+N]+.13C-NMR spectrum (125 MHz; δ, M. D.): aglycone - 61.2 (CH3, 6-och3), 94.7 (CH, C-8), 104.1 (S, 10), 116.2 (CH, C-2'), 116.9 (CH, C-5'), MEASURES 120.7 (CH, �-6'), 121.8 (C, C-1'), 132.4 (S, 6), 137.5 (S, 3), 146.3 (C, C-3'), 148.1 (S, 2), 148.7 (C-4'), CHENGDE 151.7 (S, 5), 152.9 (S, 9), 156.9 (S, 7), 177.3 (S, 4); glucose - 60.8 (CH2, C-6”), 70.3 (CH, C-4”), 74.0 (CH, C-2”), 77.4 (CH, C-3”), 78.0 (CH, C-5”), 101.1 (SN, S-1”).

The rationality used allocation scheme is confirmed by HPLC. Patuletin and patuletin are dominant components of the marginal lobes of T. patula (Fig.2). Patuletin as lipophilic flavonoid possesses great affinity for such non-polar extractive solvent as chloroform, in connection with which is ensured by its selective removal. The content patuletin in chloroform fractions of T. patula is 75-80% (mass fraction) (Fig.3a). The use of recrystallization from a mixture of chloroform - ethanol (5:1) allows to obtain the target product content ≥98% (Fig.3b). After removal patuletin of raw materials for the extraction of more hydrophilic patuletin used 90% ethanol, which allows to extract up to 96% of the compound (Fig.4A). Recrystallization of a technical product from a mixture of ethyl acetate - ethanol (3:1) was obtained target compound with a purity of ≥98% (Fig.4B).

Method for the quantitative analysis of the substance;

About 10 mg (a precisely weighed) of the substance is placed in a flask with a ground joint with a capacity of 10 ml, dissolved in 5 ml of methanol and the volume was adjusted solution to the mark with the same solvent. 1 ml of the resulting solution was filtered through quartz fil�p Millipore (⌀0.45 µm) in a chromatography vial and used for analysis nl HPLC-UV. The conditions of HPLC-UV: high performance liquid chromatograph " milichrom f-02 (EcoNova); column (ProntoSIL-120-5-C18 AQ (2×75 mm, ø 5 mm; Metrohm AG); mobile phase: (4.1 M LiClO4in 0.1 M HClO4):H2O 5:95 (A), MeCN (B); gradient mode B in A: 10-100% (0-20 min); ν 200 µl/min; Tcolumn408°C; Vsamples1 µl; detector at 360 nm.

The content patuletin (patuletin) in substance in percent (X) is calculated by the formula:

X=S1K1Vm1m2S2K2V100100-W100,

where S1- the peak area patuletin (patuletin) in the test solution;K1V- the dilution factor of the investigated solution (10); m1- the weight of the portion of the substance, g; S2- the peak area patuletin (patuletin) in the solution of the RIS; ofK2V- the dilution factor of the solution of the RIS patuletin (patuletin) (1); m2- the weight of the portion patuletin (patuletin), g; W - loss in m�CCE when dried substance %.

Preparation of solution RDF patuletin (patuletin)

About 1 mg (a precisely weighed) patuletin (patuletin) are transferred into the Eppendorf tube, pour 1 ml of methanol and stirred.

Metrological analysis of the developed technique showed that the relative error of the determination of the chromatographic method does not exceed 3%. The findings indicate a satisfactory validation parameters of the methods, which indicates the possibility of their use in practice pharmacopoeial analysis to determine indicators of quality substances patuletin and patuletin.

2.05
Table 1
Metrological characteristics of methods of quantitative analysis patuletin and patuletin in substance*
Connectionx, %S2Sx±Δx, %E%
patuletin98.520.641.791.82
patuletin98.942.910.762.122.14
*n=5, P=0.95, tP,f=2.78.

Studies have identified common indicators of quality substances patuletin and patuletin summarized in tables 2 and 3. For standardization of substances proposed definition appearance, authenticity, the loss in mass on drying, a quantitative determination of the content of the basic substance in the substance and content of impurities.

To calculate the economic feasibility of the proposed method is currently not possible because of the lack of commercially available samples of comparison patuletin and patuletin, however, the foregoing advantages, in combination with a simple scheme for obtaining contribute to the rational use of medicinal plant raw material and determine the prospects of implementation of this method of obtaining chemical industry.

Table 2
Indicators of quality substance patuletin
IndicatorMethodNorm
Description, exterior signsVisual, organolepticCrumbly nephroscopy amorphous powder, colour from yellow to greenish-yellow, no odor.
Authenticity:
- UV spectrumSpectrophotometricThe ultraviolet spectrum of a 0.001% solution patuletin in 96% ethanol in the region of wavelengths from 200 to 500 nm should contain absorption maxima at wavelengths of 258±2 and 370±2 nm. On the chromatogram after development should be the only yellow zone, coinciding with mobility such RDF patuletin; the presence of other zones is not allowed.
- patuletin-WATCH
Loss in weight on dryingGravimetricNo more than 5%
Quantitative definition:
- content patuletin.
HPLCNot less than 98%
ImpuritiesHPLCOn the chromatogram obtained in the quantitative analysis of the substance, there should be only one dominant peak with tR ~ 6.50-6.53 min. total area of the other peaks should not exceed 2%.
StorageIn the dark place at a temperature of 12-15°C

Table 3
Indicators of quality substance patuletin
IndicatorMethodNorm
Description, exterior signsVisual, organolepticCrumbly nephroscopy amorphous powder, yellow color, no odor.
Authenticity:
- UV spectrumSpectrophotometricThe ultraviolet spectrum of a 0.001% solution patuletin in 70% ethanol.�STI wavelengths from 200 to 500 nm should contain absorption maxima at wavelengths 259±2 and 372±2 nm. On the chromatogram after development should be the only yellow zone, coinciding with mobility such RDF patuletin; the presence of other zones is not allowed.
- patuletin-WATCH
Loss in weight on dryingGravimetricNo more than 5%
Quantitative definition:
- content patuletin.
HPLCNot less than 98%
ImpuritiesHPLCOn the chromatogram obtained in the quantitative analysis of the substance, there should be only one dominant peak with tR~4.65-4.69 min total size of other peaks should not exceed 2%.
StorageIn the dark place at a temperature of 12-15°C

Brief description of the drawings

In Fig.1 shows structural formulas patuletin (a) and patuletin (b).

In Fig.2 shows a chromatogram of an alcohol extract of boundary petals of Tagetes patula on which numbers are marked connections: 1 - patuletin, 2 - patuletin.

In Fig.4 shows the chromatogram of the ethanolic fraction of boundary petals of Tagetes patula (a) and patuletin (b) showing the position patuletin (7).

Sources of information

1. Park E. J., Kim Y., Kim J. Acylated flavonol glycosides from the flower of Inula britannica II Journal of Natural Products. 2000. Vol.63. P. 34-36. - the prototype.

2. Schmeda-Hirschmann G., Tapiaa A., Theoduloz C, Rodriguez J., Lopez S., G. E. Feresin Free radical scavengers and antioxidants from Tagetes mendocina II Zeitschrift fur Naturforschung. 2004. Vol.59c. P. 345-353. - the prototype.

3. Guinot P., Gargadennec A., Valette G., Fruchier A., C. Andary Primary flavonoids in marigold dye: extraction, structure and involvement in the dyeing process // Phytochemical Analysis. 2008. Vol.19. P. 46-51. - the prototype.

4. Faizi S., H. Siddiqi, A. Naz, S. Bano, Lubna Specific deuteration in patuletin and related flavonoids via keto-enol tautomerism: Solvent - and temperature-dependent 'H-NMR studies // Helvetica Chemie Acta. 2010. Vol.93. P. 466-481.- as a prototype.

5. Kashchenko N. And., Tancheva L. M., Oleynikov, D. N. Phenolic compounds and polysaccharides inflorescences Tagetes patula II. Proceedings of the V all-Russian conference with international participation "New advances in chemistry and chemical technology of plant raw materials". 24-26 April 2012, Barnaul: publishing house of the Altai University, 2012. P. 262-263.

Method for simultaneous obtaining of two flavonoids - patuletin and its 7-O-β-D-glucopyranoside - patuletin by extraction of the flowers of the marigold open, characterized in that the milled edge petals vysokoplodorodnyh varieties of marigolds locatio�ostertag placed in a Soxhlet apparatus, pour the hexane in the ratio raw : extractant 1:3 and extraction is carried out at 60°C for 36 h, after extraction with hexane and dried repeat the extraction of raw materials chloroform in the ratio raw : extractant 1:(3.5-4.5) in the same conditions, the obtained chloroform extract was concentrated in vacuo at 60°C until complete removal of the solvent, then dry the residue dissolved in a mixture of petroleum ether - chloroform 1:1 when heated to 40°C and leave at 4°C for 36-40 hours, then the precipitate was filtered off, washed with petroleum ether and dried in vacuo, the obtained dry powder was dissolved in a mixture of chloroform - ethanol 5:1 and left at 4°C for 24-30 hours, then the precipitate was filtered off, washed with petroleum ether and dried under vacuum to give patuletin; further subjected to extraction of raw materials remaining after treatment with chloroform, and the extraction is carried out with 90% ethanol in the ratio raw : extractant 1:(15-20) three times at a temperature of 85-90°C for 90 min, the obtained ethanol extract was filtered, pooled and concentrated in vacuo to 1/50 of the original volume, then the aqueous residue was subjected to liquid-phase extraction by ethyl acetate in the ratio of the residue : ethyl acetate 1:(0.3-0.5), then the organic layer is taken and repeat 3 times LM�Covasna extraction of the water layer, next, the combined organic layer was concentrated in vacuo to remove the solvent, the dry residue dissolved in a mixture of chloroform - ethyl acetate 1:2 in ratio 1:1 when heated to 50°C and leave at a temperature of 48°C for 36-40 hours, after which the precipitate was filtered off, washed with cooled to 1-2°C ethyl acetate, 0.1% solution of hydrochloric acid in 96% ethanol, 96% ethanol and ethyl acetate and dried in vacuo, the dry powder was dissolved in a mixture of ethyl acetate - ethanol 3:1 and leave at 4°C for 24-30 hours, the precipitate was filtered off, washed with ethyl acetate and dried, yielding patuletin.



 

Same patents:

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry, namely to a method for preparing lappaconitine hydrobromide (versions) A method for preparing lappaconitine hydrobromide is implemented by extraction of Aconitum leucostomum root and herb or Aconitum septentrionale root and herb in methylene chloride in a continuous extraction apparatus that is followed by decontamination by means of flash chromatography (version 1), or extraction of a herbal raw material in a polar organic solvent followed by extract removal from the organic solvent (version 2), alkalinisation and extraction of the prepared extract in methylene chloride followed by decontamination of the extract by flash chromatography.

EFFECT: method for preparing lappaconitine hydrobromide provides simplifying the technological process, reducing its length and improving higher yield of the end product of officinal purity.

7 cl, 1 tbl, 9 ex

FIELD: food industry.

SUBSTANCE: method for production of Siberian cedar seeds liqueur (with hepatoprotective, antioxidant, antihypoxic, hypolipidemic effect) by way of maceration with ethyl alcohol usage; whole Siberian cedar seeds are loaded into the reactor, poured with 70% ethyl alcohol water solution; extraction is performed under preset conditions. The medicinal preparation with hepatoprotective, antioxidant, antihypoxic, hypolipidemic effect contains Siberian cedar seeds liqueur. Usage of the medicinal preparation as a hepatoprotective remedy.

EFFECT: liqueur has pronounced hepatoprotective, antioxidant, antihypoxic and hypolipidemic effect.

6 cl, 3 dwg, 8 tbl

FIELD: medicine.

SUBSTANCE: method for producing a pigment complex of bisnaphthazarin for preventing inflammatory diseases, involving demineralising commercial sea urchins' crusts and needles in an organic acid solution, separating organic acid salts and protein, applying pigment solution on a chromatographic column, washing the column with diluted mineral acid and distilled water, eluting the pigment complex, combining fractions containing the pigments, removing ethanol, lyophilising concentrate in the certain environment. The complex of pigments bisnaphthazarins for preventing inflammatory diseases.

EFFECT: complex of pigments prepared by the above method is effective for preventing the inflammatory diseases.

3 cl, 2 dwg, 2 tbl, 4 ex

Vibration extractor // 2545300

FIELD: machine building.

SUBSTANCE: for extraction (leaching) of the substances extracted from the plant materials in food, chemical-pharmaceutical and other industries, for output increasing of the substances extracted from the extractable plant materials and for increasing of their concentration in the ready extraction it is suggested to provide the extractor with the extractant recirculation circuit containing devices for solid phase separation, pump, discharge tank, flowmeter, shutdown valves system, and in the extractor bottom part additionally union will be installed for continuous liquid phase supply.

EFFECT: wider possibility of return in the vessel of part of extraction after solid phase separation in specified ratio with fresh extractant, thus improving conditions of mass exchange due to decreasing of the surface tension of the liquid phase.

1 dwg

FIELD: medicine.

SUBSTANCE: method for preparing an agent possessing anti-inflammatory, diuretic and antioxidant activity, involving milling Spiraea salicifolia shoots representing a mixture of leaves, blossom and shoots, extracting them three times by gradual maceration, mixing in infusing, filtering, condensing, separating, drying in the certain environment.

EFFECT: agent shoes the pronounced anti-inflammatory, diuretic and antioxidant activity.

2 dwg, 12 tbl

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical and consumer industry in preparing dry herbal extracts used for later colouration of textile fabrics with this extract. A method for preparing the dry herbal extract of St. John's wort involves grinding herbal raw material, extracting in water, filtering, boiling down in a rotary evaporator and drying the residual product to constant weight in a drying chamber in the certain environment.

EFFECT: method described above enables preparing the extract with dry colorant weight 25% of dry herbal raw material weight.

FIELD: chemistry.

SUBSTANCE: method of obtaining sanguinarine and chelerythrine sulphates includes extraction of milled overground part of macleaya microcarpa and/or macleaya cordata with water aliphatic alcohol, removal of water aliphatic alcohol in vacuum, alkalinisation of water distillation residue with hydrophobic solvent, processing organic phase with sulphuric acid, filtration, washing and drying of target product, with extraction of milled raw material being carried out with water aliphatic alcohol in presence of methanesulphoacid, and solution of alkaloid bases in hydrophobic organic solvent is additionally filtered through layer of hydrophobic solvent, and target product is subjected to boiling in acetone.

EFFECT: method makes it possible to increase quality of finished product, simplify technology of production of sanguinarine and chelerythrine sulphates, and reduce the process duration.

8 cl, 14 ex

FIELD: chemistry.

SUBSTANCE: method of bee pollen processing consists in the fact, that bee pollen is extracted with CO2 by pumping CO2, obtained fat extract is separated, remaining oil-seed meal is subjected to enzymatic hydrolysis in presence of enzyme Distizym Protacide Extra, obtained fermentolisate is separated into solid and liquid phases, solid phase is dried, liquid phase is filtered, preservative potassium sorbate and sodium benzoate are added into filtered liquid fraction under specified conditions.

EFFECT: method makes it possible to efficiently process bee pollen with obtaining fractions, characterised by microbiological purity, high degree of bioavailability, and a long storage term.

4 tbl

FIELD: chemistry.

SUBSTANCE: invention relates to versions of composition for heat transmission. One of composition versions contains (i) from about 20 to about 90 wt % of R-1234yf; (ii) from about 10 to about 60 wt % of R-134a and (iii) from about 1 to about 20 wt % of R-32. Invention also relates to a number of versions of composition application.

EFFECT: composition has lower value of global warming potential and at the same time is characterised by productivity and energy efficiency.

56 cl, 7 dwg

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry, particularly to a method for recovering pinosylvin and methylpinosylvin from common pine (Pinus sylvestris) wood. The method for recovering pinosylvin and methylpinosylvin consists in extracting ground knots of common pine (Pinus sylvestris) wood, common pine (Pinus sylvestris) in isopropyl alcohol mixed with water, separating the extract and removing the solvent.

EFFECT: method enables a higher yield of pinosylvin and methylpinosylvin from common pine (Pinus sylvestris) wood.

3 cl, 5 ex, 2 dwg

FIELD: food industry.

SUBSTANCE: imitator of odour of hallucinogenic plant i.e. Hawaiian baby woodrose for sniffer dogs training contains an inert carrier and an odour substance; the imitator is specifically as follows: the odour substance is represented by a mixture containing benzoic acid and "rose" food flavouring agent.

EFFECT: sniffer dogs preparation for the said hallucinogenic substance search.

6 cl, 7 ex

FIELD: chemistry.

SUBSTANCE: medication includes 7.0-9.0 wt % of a dried thick fraction of a product of hazel dry sublimation, 0.025-0.033 wt % of hydrochloride anaprilin, 36.0-38.0 wt % of sodium hydrocarbonate, 24.5-25.5 wt % of boric acid, 12.0-16.0 wt % of phthalic acid, 5.5-7.5 wt % of sodium carboxymethylcellulose, 0.8-1.0 wt % of sodium dodecylsulphate, 0.45-0.55 wt % of calcium stearate and 5.5-10.8 wt % of glucose.

EFFECT: invention increases the therapeutic and preventive efficiency of treatment of postnatal endometritis in cows and cow-heifers.

FIELD: personal use articles.

SUBSTANCE: group of invention relates to oral care means suitable for application with oral care appliances and their use methods. The oral care comprises the following components: 5 - 45 wt % of antibacterial agent selected from among copper (II) compounds, zinc ions sources, phthalic acid and salts thereof, hexetidine, octenidine, sanguinarine, benzalkonium chloride, domiphen bromide, alkylpyridine chlorides, iodine, sulphanilamides, bis-biguanides, magnolia extract, grapeseed extract, Augmentin, amoxicillin, tetracycline, doxycycline, minocycline, metronidazole, neomycin, kanamycin and clindamycin; 5 - 70 wt % of a flavouring agent and an orally acceptable liquid carrier The composition has certain viscosity parameters. Additionally proposed are a method for introduction of the antibacterial agent into a mammal's oral cavity using such composition; an oral care device including an oral care appliance including a vessel, positioned inside it and accommodating the said composition, as well as an applicator element and a transfer mechanism for the composition transfer from the vessel to the applicator element surface; as well as application of the said composition for introduction of the antibacterial composition into the oral cavity with the help of the above device.

EFFECT: usage of the group of inventions ensures the possibility of efficient antibacterial effect, reduction of the quantity of volatile sulphur compounds and, optionally, a cooling sensation in the oral cavity in case of usage of such insignificant quantity of the composition as 50 ml.

20 cl, 4 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: skin care compound possessing the antifungal properties, alcoholic extract of birch leaves, pine paste, tea tree, fir, lemon and eucalyptus essences, an emulsion base in certain proportions.

EFFECT: compound possesses the pronounced antifungal properties.

4 ex

FIELD: medicine.

SUBSTANCE: application of a mixture of triterpenoids, containing β-amyrin 27.48 wt %, α-amyrin 27.52 wt %, moretenol 25.52 wt %, lupeol 19.48 wt % or butyro-lignans - arctiin or arctigenin, separated from Alfredia cemua (L.) Cass., as a medication, possessing a cerebroprotective action. The said mixture of triterpenoids or butyro-lignans - arctiin or arctigenin, separated from Alfredia cemua (L.) Cass., possess the expressed cerebroprotective action.

EFFECT: increased efficiency of the medication application.

1 tbl, 2 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to an agent for treating hemorrhoid, proctitis and other proctologic inflammations. The above agent represents a suppository 1,35 - 3,65 g containing diosmin 0,3 - 0,65 g, dexpanthenol 0,05 - 0,2 g, green tea extract 0,05 - 0,2 g as active substances, and emulsifier 0,0135 - 0,1825 g and fatty acid glycerides as additives.

EFFECT: agent provides the integrated antimicrobial, local analgesic, anti-inflammatory, wound healing and adaptogenic therapeutic action.

2 cl, 3 ex

FIELD: veterinary science.

SUBSTANCE: declared are a preparation for treating calves' gastrointestinal diseases and a method of treating using the same. The preparation represents a sterile culture fluid produced by culturing the fungus Trametes pubescens (strain 0663) on standard solid and fluid media with sodium selenite and zinc sulphate additionally included into the nutrient medium; that is following by lyophilisation. A method of treating involves adding the preparation to feeding milk. The preparation is introduced in a dose of 180-300 mg per 1 kg of animal's body weight once a day for 4-5 days.

EFFECT: high therapeutic effect of the preparation.

3 cl, 18 tbl, 4 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to cosmetic industry, and represents a cosmetic skin care composition of oil-in-water emulsion containing (a) paraffin wax and/or polyethylene wax, (b) microcrystalline wax and (c) animal/vegetable wax, which as a basic ingredient contains ester of higher fatty acid containing 20 to 32 carbon atom, and alcohol containing 28 to 34 carbon atoms, and has a melting point falling within the range of 75 to 100°C, wherein the ratio of the ingredient (a) and the ingredient (b) falls within the range of 70/30 to 95/5 wt., a total amount of the ingredient (a) and the ingredient (b) falls within the range of 0.01 to 2 wt % and an amount of the ingredient (c) falls within the range of 0.005 to 2 wt %.

EFFECT: invention provides the excellent sensation of skin plasticity and elasticity with no stickiness and stability.

2 cl, 2 tbl, 8 ex

FIELD: chemistry.

SUBSTANCE: invention relates to field of cosmetology and represents placed into container composition for personal hygiene with oriented particles, including visible particles with side ratio greater than 1.5:1 and particle size by long side from 300 mcm to 1000 mcm, where planes x-y of at least 50% of said visible particles are practically parallel, parallel or coincide with planes x-y of other visible particles, where said composition includes structural material for preservation of particle orientation in space, and filling container with composition is realised under conditions of laminar flow, and method of its manufacturing.

EFFECT: invention provides presentation of compositions for personal hygiene, which deliver useful agents to skin when applied, are stable in storage and have visible peculiarities, which can release product for consumer.

18 cl, 4 dwg, 2 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: treatment is two-staged; at the first stage, gingival pockets are rinsed with a stream of 1.5% hydrogen peroxide in a cannula delivered through the gingival pockets around the teeth. If observing the exposed furcation of premolars and molars, the cannula is brought under the root furcation. Thereafter, the gingival pockets are similarly rinsed with an aqueous tincture of garden sage herb for 3-5 days, then with an aqueous tincture of camomile blossom for 3-5 days, an aqueous tincture of plantain leaves daily for the following 3-5 days; each rinsing procedure is followed by applications of cotton drains impregnated with the respective aqueous herbal tincture introduced by the cannula on gingival mucous membranes for 1 hour; that is combined with prescribing oral baths with the respective aqueous herbal tincture introduced by the cannula daily 5-7 times a day. The second stage involves instillations of Normoflorin-B or Bifidumbacterin in the cannula delivered through the gingival pockets around the teeth, brought under the furcation if observing the exposed furcation of premolars and molars; the gingival mucous membranes are laid with cotton drains with the above preparation for 2 hours daily for 5-10 days.

EFFECT: method enables providing the higher clinical effectiveness.

2 cl, 1 ex

FIELD: medicine, oncology, amino acids.

SUBSTANCE: invention relates, in particular, to the development of an antitumor preparation based on natural substances. Invention relates to an amino acid preparation comprising at least one modified essential amino acid obtained by treatment of amino acid by ultraviolet radiation (UV) at wavelength 250-350 nm for 12-80 h at temperature 15-30oC or with ozone at temperature 15-25oC. The modified amino acid has no toxicity for health cells. Also, invention relates to a method for preparing such preparation. Invention provides the development of an antitumor preparation based on modified amino acids and expanded assortment of antitumor preparations being without cytotoxicity for normal cells.

EFFECT: valuable medicinal antitumor properties of preparation.

8 cl, 4 tbl, 2 dwg, 4 ex

Up!