Bioactive hydrogel wound coating

FIELD: medicine.

SUBSTANCE: what is described is a bioactive wound coating of a hydrogel nanocomposite, which contains antimicrobial and antioxidant ingredients: silver-modified montmorillonite and fullerenol used to optimise the clinical course of the wound process, to prevent and suppress a wound infection. The wound coating can be used to treat gun-shot injuries, severe mechanical injuries, infected and uninfected wounds, including septic and persistent, granulating wounds following deep thermal, chemical and radioactive burns, in the combined therapy of trophic ulcers and bed sores at hospital, in the outpatient setting and in the field. The wound coating is elastic, not fragmented in dressing that facilitates wound care. A high sorption ability of the wound coating matrix, including of coarse-molecular ingredients of the wound effluent, provides the fast elimination of the wound bed. Using the hydrogel, i.e. possessing high degree of hydration, the wound coating meets the modern wound management in the humid medium.

EFFECT: optimum conditions for the early activation of the repair processes.

5 dwg, 2 tbl, 4 ex

 

The invention relates to medicine, namely to surgery, is intended for the local treatment of wounds in the inflammatory phase of wound healing process. Bioactive wound coating based hydrogel nanocomposite contains a complex of bioactive drugs to optimize the course of wound healing, prevention of the development and suppression of wound infection. The wound covering may be used for the treatment of gunshot wounds, wounds in severe mechanical trauma, uninfected and infected wounds, including purulent and nonhealing, granulating wounds after deep thermal, chemical and radiation burns in treatment of trophic ulcers in inpatient, outpatient and field conditions.

Well-known domestic coverage on the basis of sodium alginate, which is a natural hydrogel of origin: Coletex-gel-DNA containing Derinat (sodium desoxyribonucleate) and Coletex-gel-DNA-L" - Derinat and lidocaine. It is recommended to apply the coating for the prevention and treatment of radiation reactions and injuries of the mucous membranes (oral cavity and oropharyngeal, bladder, rectum, vagina, etc.) during and/or after the radiation therapy, and for treatment of nonhealing wounds and trophic ulcers, household burns [RF patent 2483755, LLC "Coleta�with", 2006]. However, weak wound healing activity and the absence of antimicrobial action significantly reduces the possibility of wide application of these hydrogels in medical practice.

Known dressings comprising a polymeric film coated with the surface layer of the polymer hydrogel. Film is made of a biocompatible optically transparent polymer with through holes of diameter D=0.01 to 3.0 mm and a density of N=(103=109)1/cm2and as the polymer of the hydrogel caused hydrogel based on chitosan. Dressings prevents penetration to the wound microorganisms, provides bacteriostatic action in the wound, allows you to visually monitor the processes occurring in the wound, and is conveniently attached to the wound surface [RF patent 2270646, LLC "Folium", 2006].

Common shortcomings of the presented coatings are relatively low sorption capacity, significant reduction in the strength of the matrix during the swelling, the lack of absorptive capacity in relation to macromolecular components of wound exudate and microbial flora.

The closest to the claimed invention are synthetic hydrogel coatings brand "APOLLO-PACK-AM", "APOLLO-PAK-AI", created on the basis of a copolymer of acrylamide and acrylic acid with addition of antiseptics mi�of amitina or yodovidona and the local anesthetic amylocaine. The hydrogels also have low sorption capacity of 2-3 g/G. Coatings contain antimicrobial and analgesic drugs, but do not contain antioxidant agents that significantly reduces their effectiveness in marked inflammation [TU 9393-002-42965160-2009, "th "APOLLO", 2009; http://www.appolo.ru/products/gidrogelevaya-produktsiya/povyazka-ranozazhivlyayushchaya-appolo.html#]. Essential structural disadvantage of these gels is that a significant portion of the gel, when opening the package remains on the inner surface (50%), and is not transferred to the wound with the mesh carrier. These wound dressings have a low pH at 5.5 to 6.0, in the context of local tissue acidosis also reduces their effectiveness.

Thus, the need to develop ways of immobilization of bio-active components in the matrix-media-based hydrogels of different chemical nature, which provides prolonged release of the latest in wound bed, while maintaining their high sorption capacity and mechanical strength, remains.

Technical challenge and a positive result of the present invention to provide a bioactive hydrogel wound dressings containing pathogenetically substantiated the complex of therapeutic drugs, local application which will optimise�VAT the course of wound healing, to reduce healing time, prevent the development of complications.

This problem is solved by creating based hydrogel nanocomposite wound dressings containing bioactive components: antimicrobial - modified silver-montmorillonite (Ag-MMT) and antioxidant - fullerenol.

The task and the technical (social) result is achieved due to the fact that the bioactive hydrogel wound coating contains a copolymer of acrylic acid and acrylamide, wherein the composition of the copolymer filler included-modifier-modified silver montmorillonite, which allows to obtain hydrogel nanocomposite with intercalated structure having antimicrobial activity, immobilization in a copolymer at the stage of synthesis of fullerenol coating gives the antioxidant action.

Bioactive hydrogel wound covering is characterized in that to obtain a homogeneous structure of the polymer matrix wound dressings and optimal, dosage and prolonged, use of antimicrobial action of finely dispersed fraction of the modified silver montmorillonite particles with a diameter less than 0.25 mm.

Bioactive hydrogel wound coating is characterized by the fact that the concentration of bioactive components in the matrix �leaves, wt.%:

a modified silver-montmorillonite2,0-5,0
fullerenol1.0 to 2.0

It wound coating has a high degree of hydration meets the modern requirements of wound management and provides optimal conditions for earlier activation of reparative processes, minimizing the inflammatory phase plot of damage. This allows for earlier recovery of vital functions of the damaged organ.

Hydrogel wound matrix coatings synthesized by reacting the neutralized acrylic acid (AA) and acrylamide (AA) with N,N'-methylene-bis-acrylamide (MBA) as a crosslinking agent in the presence of redox systems "ammonium persulfate - tetramethylethylenediamine (TMED)". Pre-purified acrylic acid was dissolved in water at a concentration of 30 wt.%. The neutralization was performed by sodium bicarbonate solution (NaHCO3) under the control of the pH-metry. Pre-calculated amount of a solution of NaHCO3was gradually added to establish the pH of the mixture in the range of 7.0 to 7.5. The neutralization reaction is accompanied by release of large amounts of carbon dioxide,�that after its completion the mixture was allowed to stand for 5-10 minutes. Then to the mixture was added the calculated amount of AA, MBA, TMED and water. The mixture was thoroughly stirred until complete dissolution of all components followed by re-control of pH. Thereafter, the mixture was cooled to a temperature of 5-10°C to prevent spontaneous polymerization. After cooling, the mixture is added the calculated amount of ammonium persulfate, was mixed thoroughly and poured in chemically pure form.

Introduction bioactive fillers, an aqueous solution of fullerenol and a modified silver-montmorillonite was carried out at the stage of synthesis of the hydrogel polymer, achieving a uniform disclosure of layered structures and the distribution of montmorillonite in the polymer using an ultrasonic disperser. Thus, the obtained bioactive wound dressings, representing nanocomposites with intercalated structure due to the introduction of chains of acrylamide polymer in magpakita space of montmorillonite particles.

Development and practical application of hydrogel wound dressings are particularly relevant in the light of modern principles of the wound in a moist environment. A high degree of water content of hydrogels helps soften the necrotic formations due to the rehydration of the tissues, which facilitates their mechanical removal. The creation of a moist wound �Reda provides for optimal wound healing conditions and, as a result, accelerates the processes of granulation and epithelization. Hydrogel polymers are plastic and well modeled on the wounds with difficult terrain, have no adhesion even after prolonged exposure to the wound surface and are easily removed without the risk of trauma. Significant sorption capacity of hydrogels provides elimination of the wound of necrotic tissue, microbial cells, excess wound discharge.

It is known that fullerenol along with other water-soluble clusters of fullerene C60(C60/PVP and C60/Tween 80) has demonstrated antioxidant properties at concentrations of 0.01%. The use of an aqueous solution of fullerenol in wound process will contribute to the stabilization of their own antioxidant defense system, inhibit the development of free radical lipid peroxidation, cellular and capillary membranes, preventing damage to cells and tissues, limiting the spread of the inflammatory changes and secondary necrosis of the tissues, and by enhancing the phagocytic activity of macrophages contribute to the enhancement of nonspecific immunity. Parenteral administration of fullerenol not exhibit acute toxicity, does not affect the morphological and functional condition of internal organs, metabolism and don't you�device pathological reactions of the blood.

It is known that silver has a broad spectrum of antibacterial action, also influences the growth of yeast, fungi. Certificate of identification of resistant microorganisms have a silver sporadic, which is especially important on the background of increased resistance to other traditional antiseptic. The most promising for immobilization on wound dressings are nanoscale particles of silver (AgNPs - ArgentumNanoParticles) providing a metered-dose and prolonged antimicrobial effect. Limited the interlayer space of montmorillonite, being specific "nanoreactors", can be used for the cultivation and delivery of nanoscale particles of silver.

Preclinical trials, namely the effect of local application of reticulated bioactive wound dressings, established by experiment on animals (male rats of Wistar line).

Examples of pre-clinical trials

Example 1. Biocompatibility of wound coatings was studied on the model of skin-planar conditionally aseptic wounds in 20 rats. In the postoperative period, clinical signs of inflammatory and degenerative changes in the wound is not marked. According to histomorphological studies on day 7 installed classic uncomplicated picture of the regenerative process, allowing �to conclude biocompatibility and bioinertness wound coverings.

Example 2. To determine the ability of wound dressings to inhibit the growth of bacterial flora on the border of contact with wound surface indicators installed their bacteriostatic action, as well as minimum inhibitory concentration (MIC) of Ag-MMT in their composition.

The MIC values of Ag-MMT in the matrix composition, the wound dressings on the basis DCSH acting on the reference and hospital strains of microorganisms, set at 0.1 wt.% and 0.2 wt.%. respectively.

The test results are shown in table 1 and Fig.1 - P. aeruginosa, Fig.2 - E. coli, Fig.3 - MRSA (concentration of Ag-MMT in the polymer mass.%: 1-1%, 2-2%, 3-3%, 4-5%, 5-10%).

Table 1
Evaluation of bacteriostatic action wound coverings on areas of stunted growth of microorganisms, mm
The Ag-MMT, wt.%
Strains of microorganisms
123510
ReferenceE. coli3,2±1,3a 10.4±3,3230,7±2,0434,3±2,87 36,8±3,11
S. aureus02,4±0,568,6±2,7315,7±2,6427,6±3,15
P. aeruginosa2,2±0,658,3±1,1627,5±3,2231±2,6532,8±2,81
Association05,46±1,7117,4±2,5725,3±2,4631,4±3,4
HospitalK. pneumoniae3,6±1,0612,6±1,5518,46±2,320,33±3,4824,1±3,01
P. aeruginosa02,4+1,087,4+1,6515,45±2,5321,3±2,75
MRSA00,26±0,053,76±1,4512,6±1,5418,89±2,55

Example 3. The evaluation of the ability of RA�developed coating to inhibit the growth of wound microflora found a significant reduction in the level of bacterial contamination of infected wounds, and the possibility of relatively long it is maintained below the critical level (103SOME) due to the prolonged antimicrobial action. Effective suppression of wound infection, which is the leading pathogenetic link of the complicated course of wound process, should help to reduce the duration of wound healing (Fig.4 - bioactive hydrogel wound cover, Fig.5 - aseptic gauze bandage).

Immobilization of Ag-MMT as an antimicrobial component of bioactive wound dressings based hydrogel nanocomposite in a minimum concentration of 2 wt.% allows to obtain a significant bacteriostatic effect in vitro and maintain the level of bacterial contamination of the wound below 103(CFU) for 5-7 days in vivo.

Example 4. According to planimetric studies presented in table 2, it was found that topical application of bioactive hydrogel wound dressings prevents complications during the healing process, allows to reduce the time of wound healing in the treatment of granulating wounds after deep burns and infected skin-planar RAS 15-20%.

Table 2
Indicators planimetric studies �of azulene RAS, %
The experimental group
The observation period
Granulating wounds after deep burnsInfected skin of planar-wound
ControlExperienceControlExperience
12121212
5 day20,8±1,14,2±0,227,4±0,8*5,5±0,1*29,4±1,4*5,8±0,4*43,4±0,2*8,7±0,3*
10 days42,3±2,14,2±0,163,2±1,3*6,3±0,3*58,2±0,4*5,8±0,6*89,5±2,1* 8,9±0,5*
15 days68,1±1,34,5±0,398,7±0,6*6,6±0,6*95,3±0,3*6,3±0,3*--
20 days99,3±0,34,9±0,2------
The healing periodof 20.0±0,415,8±0,7*16,0±0,413,4±0,3*
Note to table 2:
1 - reduction of the wound area as a percentage of the initial size of the wound;
2 - healing rate (%/day).
* Differences from control are significant at p<0,05.

Bioactive hydrogel wound coating containing at its core with�of OLIMAR acrylic acid and acrylamide, characterized in that at the stage of synthesis in the composition of the copolymer included two components: a modified silver-montmorillonite in the form of finely dispersed fractions with particle diameters of less than 0.25 mm at a concentration of 2.0-5.0 wt.%, allowing you to receive as a polymeric matrix coating the hydrogel nanocomposite intercalated with homogeneous structure, giving the coating is metered and prolonged antimicrobial action; and fullerenol in a concentration of 1.0-2.0 wt.%, gives the coating an antioxidant effect.



 

Same patents:

FIELD: biotechnology.

SUBSTANCE: invention is a method of producing an adjuvant for vaccines, comprising dissolving a mixture of triterpenoids of bark in tetrahydrofuran, addition of oleic acid, removal of tetrahydrofuran, addition of cryoprotectant, and lyophilisation, at that a mixture of triterpenoids of bark in tetrahydrofuran at a concentration of 5-10 g/l is obtained, followed by dissolving the resulting mixture in oleic acid in an amount of 5-10% by weight of triterpenoids of bark, sterilising filtration of the mixture is carried out, a homogeneous dispersion of spherical amorphous nanoparticles is formed by adding a 25-fold excess of 0.01 M tris buffer, pH - 9.0±0.2 with stirring, followed by ultrasonic treatment for 5-10 minutes, tetrogidrofuran is removed by ultrafiltration at a rate of 1.0-1.2 l/min at a pressure of 0.6-0.8 atm, adding the cryoprotectant the resulting concentrated mixture containing a mixture of terpenoids of 1 mg/ml with the temperature below minus 35°C is frozen, kept at this temperature for 4-6 hours, and lyophilised at a temperature of minus 35°C for 15 hours, followed by subsequent drying at 20-25°C for 15 hours.

EFFECT: invention enables to increase the immunogenic activity of viral vaccines and provides their stability while storage.

3 cl, 2 dwg, 6 tbl, 7 ex

FIELD: food industry.

SUBSTANCE: method for pre-planting treatment of potato tubers is implemented with the help of a suspension of nano-particles of iron oxy hydroxide (IOH), ultrasonically treated. IOH is isolated with water treatment wastes at artesian water deferrisation stations. IOH is used in a gel condition. The gel contains 10-12% of IOH. The IOH gel is used in the form of a 0.01-0.001% suspension, inter alia - mixed with a protectant. IOH is also used in a modified condition. The modifier is represented by PVA in an amount of 0.5-1.0% in conversion to IOH dry matter. Modified IOH can be used as mixed with a protectant.

EFFECT: invention usage will allow to ensure potato yield increase and quality enhancement.

5 cl, 2 tbl, 3 ex

FIELD: nanotechnology.

SUBSTANCE: invention relates to the field of nanotechnology, namely to electronic elements consisting of the layers and containing nanomaterials in their structure. In the electronic element, comprising layers of materials with conductive and nonconductive parts, at least one layer is made of a metal matrix composite containing 25-75% (by volume) of reinforcing particles consisting of nanodiamonds with high electrical resistance and bulb-shaped carbon nanoparticles with low electrical resistance, arranged in order providing the flow of electric current in the desired direction. In the method of manufacturing of electronic elements, consisting of layer application of required materials, at least one layer is made of a metal matrix composite produced by the method of mechanical alloying of the initial particles of the matrix material and 25-75% (by volume) of nanodiamond particles, at that first the granules of composite material are obtained, then the composite material is applied on the preliminary treated process surface, the surface alignment is carried out, then local heating of those areas that need to be conductive is carried out to temperatures exceeding 1000°C, thereby transforming nanodiamonds in bulb-shaped carbon nanoparticles.

EFFECT: reduction in the size of electronic elements.

11 cl

FIELD: physics.

SUBSTANCE: photoconverter element has a plate made of conducting material, sensitised titanium dioxide, a transparent element coated with a conducting coating. The sensitised titanium dioxide is deposited on the plate made of conducting material on both sides and is coated with a transparent element with a conducting coating.

EFFECT: invention increases the efficiency of the photoconverter element, lowers the cost and simplifies production thereof.

1 dwg

FIELD: measurement equipment.

SUBSTANCE: method to manufacture a strain gauge pressure sensor consists in polishing of membrane surface, formation of a dielectric film and strain gauge elements on it with low-resistance links and contact sites between them, using a template of a strain gauge sensitive layer in the form of strips, attachment of output conductors to contact sites in areas remote from strips of sections, connection of a recorder to the output of nano- and microelectromechanical systems (N&MEMS), energising of N&MEMS voltage, development of a normalized non-stationary field of temperatures and temperature deformations symmetrical relative to the centre of the membrane on the membrane. The non-stationary field of temperatures and temperature deformations is recorded on the recorder of the output signal of N&MEMS during impact at the membrane. They compare the produced output signal of the tested N&MEMS according to the amplitude of spectral components with the similar signal of the reference N&MEMS. If differences of amplitudes of output signals or amplitudes of spectral components of output signals do not exceed limit permissible values, which are not taken as criteria of time stability, this assembly is forwarded to subsequent operations.

EFFECT: increased time stability, resource, service life, reduced error under impact of non-stationary temperatures and increased vibration accelerations.

2 cl, 2 dwg

FIELD: physics.

SUBSTANCE: radiator contains a heat-conducting substrate on the working surface of which the parallel extended structures in the form of ledges of prismatic shape are formed which have alloyed surface layers with considerably larger electric conductivity, than the substrate. The extended structures are connected to the conducting contact areas formed on the substrate. Surfaces of the substrate and the heat-emitting structures are covered from above with a nanodimensional layer of silicon dioxide.

EFFECT: improvement of reliability, specific acoustic power and maximum frequencies of radiated acoustic oscillations.

1 dwg

FIELD: construction.

SUBSTANCE: complex admixture for concrete mix contains Giperlit hyperplasticising agent and Taunit carbon nanostructured material (CNM) at the following component ratio, wt %: Giperlit hyperplasticising agent - 99.5-99.7, Taunit carbon nanostructured material - 0.3-0.5. A preparation method of the proposed complex admixture consists in ultrasonic dispergation of Taunit carbon nanostructured material in Giperlit hyperplasticising agent during 3-7 minutes by means of UZG13-0.1/22 ultrasonic generator.

EFFECT: increasing growth rate of strength of concretes at early hardening stage at preservation of their high final strength; increasing freeze resistance and water non-permeability.

2 cl, 1 tbl

FIELD: physics.

SUBSTANCE: method is based on irradiating a surface with a moving laser beam in areas of the surface of the material with an absorption coefficient of not less than 3·104 cm-1 at the laser wavelength, wherein each area is irradiated using a series of laser pulses with pulse duration of not more than 30 ns, and energy density F of the laser beam in the irradiated area is set in the range F=0.005-1.0 J/cm2 to enable splitting of the surface layer of the material without melting and forming on the surface of the material sub-micrometre cracks, slits and flakes with size ranging from 0.05 mcm to 0.8 mcm.

EFFECT: simplified method of producing micro- and nanostructures for a wide range of materials.

12 cl, 1 tbl, 7 dwg

FIELD: physics, signalling.

SUBSTANCE: invention relates to identification of material resources and can be used to label electroconductive articles. The method of making and installing non-reproducible identification label on an electroconductive article includes applying an identification number, an information grid and a non-reproducible matrix, as well as combined input of the identification number and the non-reproducible matrix into a database. The non-reproducible matrix is formed in advance separately from the article on a nanofilm by random point-by-point evaporation of areas of the nanofilm to obtain perforations of different size and shape or obtaining bulging areas of the surface of different size and shape on the nanofilm during electric discharge treatment thereof, after which the nanofilm is placed on the article by pressure sintering.

EFFECT: invention enables to obtain a non-reproducible identification label on an article, which enables to apply information and prevent forgery.

3 cl, 6 dwg, 4 ex

FIELD: chemistry.

SUBSTANCE: invention relates to the purification of finely-disperse organic substances from water-soluble admixtures and can be applied in the chemical, petrochemical, pharmaceutical, food branches of industry. Described is a method of removing water-soluble admixtures from suspensions of organic products by repeated repulpation-decantation with the application of water, containing metal nanoparticles, as a washing liquid. At the first and second stages of the repulpation used is water, passed through a layer of carbon with high reaction ability, containing nanoparticles of metal oxide from the group, including nickel oxide, nickel and chrome oxide, nickel and iron oxide. At the third stage of the repulpation used is water, containing nanoparticles of metal from the group, including iron, aluminium, nickel, platinum. The fourth repulpation is carried out with the application of water, containing a micellar solution of silver or gold as a washing liquid.

EFFECT: invention provides the reduction of loss of the target substance with a washing liquid, reduction of the volume of sewage waters, requiring purification from organic compounds and water-soluble admixtures and increase of the consumer properties of organic substances.

10 cl, 3 dwg, 4 tbl, 14 ex

FIELD: medicine.

SUBSTANCE: wound surface is treated with 3.0-1.5% hydrogen peroxide; then keeping the wound wet, Ichthyosin dressing prepared by streptocide powder 0.5-2.5g and Ichthyol ointment 5.0-10.0g dissolved in castor oil 94.5-87.5ml is applied. The agent is applied once a day; the therapeutic course makes 16 days. That is combined with performing an integrated treatment.

EFFECT: invention provides both the antibacterial and anti-infectious, and local anti-inflammatory, local anaesthetic, wound-healing effects, improves the blood supply, stimulates the epidermis regeneration, intensifies the keratosis processes that enables accelerating the wound cleansing from the purulonecrosis process, and a length of preparation for aurografting.

1 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: group of inventions concerns a method for preparing Sostakovsky balsam containing mixing vinyl butyl ether and butyl alcohol, heating a reaction mass while stirring continuously, feeding a catalyst after achieving the required temperature, decontaminating the prepared polymer, pouring out the polymer heated to 50-70°C into a container through a multilayer filter; the above group also concerns the therapeutic agent possessing the wound-healing, bacteriostatic and analgesic properties and prepared by the above method.

EFFECT: higher yield and purity of the produced preparation as compared to the similar methods with preserving the pharmacological properties inherent to the given preparation.

5 cl, 1 ex

FIELD: medicine.

SUBSTANCE: treating trophic ulcers is ensured by using autoserum prepared of blood 20.0-25.0 ml; centrifuging and separating it from a protein-thrombocyte clot are followed by edge chipping under a wound bed, while the protein-thrombocyte clot is used to be laid on the wound directly. The above procedures are repeated up to 20 times every two days.

EFFECT: invention enables providing fast wound healing with the adequate recovery of the dermal skin layer by using the high-biocompatibility material containing the ingredients creating the optimum medium for the wound regeneration.

2 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to the pharmaceutical industry and represents a therapeutic agent used for treating or preventing palmar-plantar erythrodysesthesia syndrome (PPES) induced by a multifunction kinase inhibitor (MKI) therapy and containing a therapeutically effective amount of allopurinol or its pharmaceutically acceptable salt.

EFFECT: invention provides extending the range of products for treating or preventing palmar-plantar erythrodysesthesia syndrome (PPES) induced by the multifunction kinase inhibitor (MKI) therapy.

21 cl, 5 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to the pharmaceutical industry, particularly to a preparation possessing anti-inflammatory, analgesic and wound-healing action. The preparation possessing the anti-inflammatory, analgesic and wound-healing action represents a mixture of an alcoholate of lilac blossom and plantain leaves with honey and extracted juice of unpeeled pomegranate.

EFFECT: preparation possesses the pronounced anti-inflammatory, analgesic and wound-healing action.

6 ex

FIELD: medicine.

SUBSTANCE: group of inventions refers to medicine and can be used for treating an individual having a slowly healing or chronic wound. That is ensured by administering a composition containing therapeutically effective amounts of an anti-connexin43 agent, and a protein or a peptide effective for stimulating or improving the wound healing into the individual in need thereof. What is also presented is using the therapeutically effective amount of a first wound-healing compound and a second wound-healing compound, wherein the above first wound-healing compound represents the anti-connexin43 agent, while the above second wound-healing compound is specified in a group consisting of beta-adrenergic antagonists, interleukin-1 receptor antagonists, free radical scavengers, anti-inflammatory and antimicrobial agents, opioids, connexin phosphorylation agents.

EFFECT: group of inventions provides higher rate and/or quality of wound healing by means of using the above combination of the therapeutic agents when it is possible to reduce the dose and rate of administration.

46 cl, 19 dwg, 8 tbl, 11 ex

FIELD: medicine.

SUBSTANCE: invention refers to biotechnology, specifically to drug preparations of erythropoietin (EPO) for the regeneration of injured tissues, and can be used in medicine. EPO is used for producing a drug preparation for a patient's structural neogenesis. If a wound represents a burn injury, EPO is applied locally on the burn wound by a skin graft to be applied on the above wound treated by EPO. Skin diseases require the local introduction of EPO into the blood coagulate of a wound bed which is mechanically pre-treated before EPO is applied, or EPO is introduced as a part of a slow-soluble hydrogel of fibrin, polymer or alginate by the local application of the above hydrogel.

EFFECT: invention enables accelerating the granulation tissue formation accompanying the patient's structural neogenesis and ensuring the skin burn healing with no scarring.

11 ex

FIELD: medicine.

SUBSTANCE: therapeutic agent contains carboxymethyl cellulose sodium salt as a base and a combination of antiseptic, 0.01% Myramistinum and metronidazole as therapeutic ingredients. The invention provides preparing the therapeutic agent possessing the antiseptic, wound-healing and sorption action on local pyoinflammatory processes in soft tissues and mucous membranes, used in surgery, dermatology, obstetrics and gynaecology, otorhinolaryngology.

EFFECT: agent possesses the higher efficacy.

2 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: therapeutic agent contains an alloy of polyethylene oxide of molecular weight 400 and 1500 as a base and comprises a combination of antiseptic, benzalkonium chloride and metronidazole as therapeutic ingredients. The invention provides preparing the therapeutic agent possessing the antimicrobial, sorption and wound-healing action on local pyoinflammatory processes in soft tissues and mucous membranes, used in surgery, dermatology, obstetrics and gynaecology, otorhinolaryngology.

EFFECT: agent possesses the higher efficacy.

2 tbl, 3 ex

FIELD: veterinary medicine.

SUBSTANCE: preparation for treatment of infected wounds in the toe region of animals, containing dimethyl sulphoxide, methyl cellulose, additionally comprises tylosin tartrate, sulphadimine, trimethoprim in the following ratio of components, wt %: tylosin tartrate - 2-3, sulphadimine - 4-5, trimethoprim - 1-1.5, dimethyl sulphoxide - 10-15, methylcellulose - 3.0-3.5, water - the rest. The claimed method comprises applying the claimed preparation without preliminary wound cleaning. The preparation is applied to the wound with the layer thickness of 2-3 mm, without application of soft dressing. The treated animals are kept for 2-3 hours on the dry surface.

EFFECT: use of claimed group of inventions increases the efficiency of treatment.

4 cl, 7 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: what is described is a mesh bioactive wound coating with its base containing disintegrated bacterial cellulose comprising antimicrobial and antioxidant ingredients: silver-modified montmorillonite and fellerenol aiming at optimising the course of the wound process, preventing the development and suppression of a wound infection. The mesh bioactive wound coating is used for treating gunshot wounds, severe mechanical injuries, uninfected and infected wounds, including septic and persistent wounds, granulating wounds following deep thermal, chemical and radiation burns, for conducting the integrated treatment of trophic ulcers and bedsores in hospital, out-patient and field settings.

EFFECT: mesh bioactive wound coating is non-toxic; it causes no local irritant and skin re-absorption action, possesses elasticity, a high degree of wound modelling; it is not fragmented that facilitates a wound care.

5 dwg, 2 tbl, 3 ex

Up!