Composite ceramic bone grafts made of ceramic material of zirconium oxide - aluminium oxide system
SUBSTANCE: invention describes a composite ceramic bone graft containing a porous ceramic carrier made of zirconium oxide - aluminium oxide; the carrier is coated with a layer of hydroxyl apatite and platelet-rich plasma; the carrier is made by preparing a mixture ceramic powder and foaming agent Al(OH)3 or Zr(OH)4, adding distilled water to give the moulding properties and caking the end product.
EFFECT: composite ceramic bone graft of the ceramic material of zirconium oxide system is effective and applicable in medicine for synthesis of the anatomic continuity and functions of the bone tissue.
1 ex, 14 dwg
The invention relates to medicine, namely to traumatology, orthopedics, regenerative medicine, dentistry and maxillofacial surgery to restore the structure and function of bone tissue.
The actual problem in medicine is the restoration of the anatomical integrity and function of bone tissue. One of the reasons for the disability population are diseases and post-traumatic conditions, coupled with the need for reconstruction and replacement of sections of bone tissue, prosthetic fragments of the musculoskeletal system. Bone defects were formed after injuries, surgeries, tumors, require a full plastic materials. Duration of treatment patients and their disability ranges from 6-8 months to 2-3 years, and 6-8% of cases end in a permanent disability that entails significant economic costs and social significance. Hence the importance of early research management capabilities reparative processes. The timing adjustment through an adequate choice of materials or the creation and use of materials with desired set of properties.
Currently, medicine is known for a wide range of upper-matrices. The greatest interest for tissue engineering represent a mule is thepotential stromal cells in the bone marrow (MSC), capable of differentiation into osteogenic, chondrogen and adipokines directions . Introduction cells leads to the replacement of lost cellular elements, induction of regeneration by releasing growth factors and differentiation, formation of extracellular matrix, which greatly speeds up the recovery of damaged tissue . Immobilization of cultured cells on the matrix carrier provides the highest concentration of cells in the damaged area, differentiation in a given direction and increases the survival rate of the cells during transplantation.
Demineralized bone matrix (DCM) due to natural architectonics, osteogenic potential, due to the presence of biologically active substances, is one of the most popular materials for osteoplasty. Shows the effectiveness of media-based DCM with immobilized MSC recovery defects of the parietal bones in rats and dogs . Patented technology for the treatment of false joints using biotransplant based DCM and autologous MSCS . The disadvantages of this xenogenic material are the need for handling aggressive fluids (reduce cell adhesion), potential immunogenicity and the possibility of transfer of infectious diseases.
Shiro is used on synthetic biodegradable carriers on the basis of polylactic and polyglycolic acids and their copolymers in combination with MSC also demonstrate the induction of osteogenesis in vitro and formation of bone tissue through 6-9 weeks in experiments on animals . Membrane of polylactide and/or polyglycolides, impregnated with chondrocytes or bone marrow MSC, patented as a method of treatment of bone and cartilage defects in humans and animals . The disadvantages of such media is quick and not always predictable degradation of the matrix, which is accompanied by a local decrease of pH in the field of implant that reduces the effectiveness of their use .
Known biotransplant based on highly porous ceramic material system Zirconia - alumina and multipotent stromal cells of human bone marrow to restore long bone defects (patent RF № 2386453, 2008) adopted for the prototype, characterized in that it contains autologous or donor multipotential mesenchymal stromal cells (MSC) from the bone marrow and the media created on the basis of a highly porous ceramic material system Zirconia - alumina technology duplication polyurethane basis, with the media densely seeded MSC, cultured from 1 to 3 passages, at the same time on the same carrier immobilized from 200 to 500 thousand cells, the vitality of which is at least 90%, and functional orientation is confirmed by the ability of the directed differentiation of IU is dermaline line and demonstrates the expression of stromal markers CD 90, CD 105, 60-90% of the cells and the absence of expression of the marker CD 34.
However, working with multipotent mesenchymal stromal cells (MMSC) is very laborious and requires a considerable period of time from 2-3 weeks up to 1 month from the period of sampling from the patient prior to implantation. In addition, when the number of passages in cell culture, an increasing number of mutations.
Disclosure of the invention.
The technical result of the invention is the creation of a composite bone-ceramic implant-based ceramic material system Zirconia - alumina, which optimizes the process of repair and restoration of damaged bone structure. Increases biological compatibility, strength properties. Ensures the absence of immune response. Enhances the reparative processes in the damaged tissues. Provides fast healing and restoration of bone structure.
This technical result is achieved in that the composite bone-ceramic implant containing porous ceramic media based on Zirconia - alumina in the carrier coated with the layer of hydroxyapatite and platelet-rich plasma, while the carrier is obtained by preparing a mixture of ceramic powder based on ZrO2(Mg, Y) or Al2About3and additives of plastipak the ora and pore-forming Al(OH) 3or Zr(OH)4followed by adding distilled water to give a mixture of molding properties and sintering the finished product.
The invention is illustrated in the following graphic materials, where Fig.1 - General view of the product.
A variant embodiment of the invention.
Step 1. The manufacture of porous ceramic material. The manufacture of porous ceramic material is carried out in a known manner by the RF patent № 2476406, 2008, by preparing a mixture of ceramic powder and an additive acting as a plasticizer and blowing agent, forming the desired product configuration and subsequent sintering, as the ceramic powder uses ultra-fine powder of solid solutions on the basis of ZrO2(Mg, Y) or ultra-fine powder of Al2O3and as a plasticizer and blowing agent use the Hydrosol Al(OH)3or Zr(OH)4in an amount of from 1 to 50% by volume of the mixture, add, stirring, distilled water to give a mixture of molding properties, then hold the molding by pressing under a pressure of from 12 to 25 kN, and sintering the product is carried out at a temperature 1450-1600°C.
Step 2. Manufacturer WPC - sawdust further biological hydroxyapatite. Made of biological hydroxyapatite in a known manner by the RF patent№ 2232585, 2001 by cleaning the bones, sawing into fragments, washing with water and deproteinization at 37°C with 0.01% solution himopsina within 72 h and 10% hydrogen peroxide solution for 48 h, the processing liquid ether, 10% solution of lithium chloride for 16 h and washing with sterile distilled water, deproteinizing bone after washing with sterile distilled water crushed to particle size of 0.1-0.5 mm, obtained deproteinizing bone meal are combined at a temperature of 38-40°C with 9% gelatin, prepared on preserving solution with the addition of antibiotic, preserved at -20°C and sterilized.
Step 3. The coating of biological hydroxyapatite. On the composite bone-ceramic implants is a cold gas-dynamic spraying of biological hydroxyapatite by installing a cold gas spraying (CS) with the following parameters: working gas is air; the pressure in the prechamber 0.3 to 5 MPa; the temperature in the prechamber 0-1000°C; gas Flow rate ≤1.5 m3/min; power consumption ≤20 kW.
Step 4. Application of platelet-rich plasma on the composite bone-ceramic implant with a coating of biological hydroxyapatite. 20-30 minutes before operations are performed blood sampling 10-15 ml of the patient in a vacuum tube. The tube is blood centrifuged for medical centrifuge at 1500 rpm for 15 minutes. In the centrifuge process is the separation of blood into fractions with the formation of the median fraction on the border between erythrocytes and plasma. The resulting platelets are extracted with a syringe in a sterile Petri dish, where directly immerse the implant.
Experimental study. Implantation of experimental samples of materials were carried out in the compact bone defect Mature rats male Wistar rats at the age of 5-6 months, weighing 260-320 grams. Model perforating defect of the mandible with the subsequent filling of the defect with the materials used for the study of biological response for the experimental sample.
Composite bone-ceramic implants based on Al2About3and ZrO2-ceramic, cylindrical shape with a height of 2-3 mm, diameter 2 mm and coated with a biological hydroxamate. To stimulate bone formation was used platelet-rich plasma (PRP). Animals were taken from the experiment on the 15th and 30th day. Thus were formed the following groups/
In the first series - studied biological response upon implantation in the compact bone of experimental samples of bone-ceramic implants, i.e., Zirconia ceramics with surface coating deproteinisation holocaustum powder. Number of animals - 6, to icesto operations - 12. The follow-up period was 15 and 30 days after implantation.
In the second series studied biological response upon implantation in compact bone of the experimental modified samples of bone-ceramic implants, i.e., samples modified platelet-rich plasma. Number of animals - 6, number of operations 12. The follow-up period was 15 and 30 days after implantation.
Experimental studies were performed according to the elaborated methods and protocols "NIETO them. J. L. Ziviani" Ministry Of Health Of Russia.
The results of the first series of experiments, after 15 and 30 days after implantation.
1.1. Composite bone-ceramic implants in 15 days after implantation. In Fig.2 presents a composite bone-ceramic implant in the perforation defect of the mandible in 15 days after implantation. White arrows indicate the bone tissue on the surface of the sample, black - bridges between the edge of the bone defect and the newly formed bone. In Fig.3 presents extracted from the bones of an animal bone-ceramic implant. On the surface of the bone-ceramic implant - the newly formed bone tissue, painted in the color purple. Quite a large array of newly formed bone with formation of young bone beams.
1.2. Composition to the IDT-ceramic implant 30 days after implantation. Composite bone-ceramic implant 30 days after implantation is shown in Fig.3, Fig.4, Fig.5. In Fig.4 presents the appearance of a composite bone-ceramic implant is implanted in the lower jaw of the experimental animal. Observed the formation of newly formed bone at the periphery of the implant and on its surface. In Fig.5 presents the appearance of a composite bone-ceramic implant extracted from the jaw bone of an experimental animal. Himself composite bone-ceramic implant presents white. On its surface are dark blotches corresponding newly formed bone on the porous surface of the implant. In Fig.6 shows the appearance of the bone defect after removal of her implant. Edge of the bone defect rather rounded, correspond to the shape of the implanted composite bone-ceramic implant. Sometimes small bumps corresponding to the forming bone "bridges" to the pores of the composite bone-ceramic implant. In the lower part there is a configuration change of bone defects due to formation of surface layers on the implant, directed from the periphery to the center of the formed bone defect.
The results of the second series of the experiments after 15 and 30 days after implantation.
2.1. Composite bone-ceramic implant with a modified enriched vombatidae plasma within 15 days after implantation. The appearance of the composite bone-ceramic implant with a modified platelet-rich plasma, after 15 days after implantation into the bone of the lower jaw of the experimental animal (rat) looks as shown in Fig.7 and Fig.8. Some sites of bone resorption, places, sites of deposition of the newly formed bone on the composite bone-ceramic implant with a modified platelet-rich plasma. Strata to reach from the bones of the lower jaw to the center of the implant. In Fig.9 and Fig.10 presents extracted from the bones of the lower jaw composite bone-ceramic implant with a modified platelet-rich plasma. There are clear signs of biological compatibility of the implant, which is expressed in the ability to the formation on the surface of young bone, dyed in purple color when counterstaining with hematoxylin and eosin
2.2. Composite bone-ceramic implants with a modified platelet-rich plasma after 30 days after implantation. The appearance of the composite bone-ceramic implant with a modified about kamennoi platelet plasma after implantation into the bone of the lower jaw of the experimental animal (rat) looks like this, as is shown in Fig.11 and Fig.12. Newly formed bone was almost completely covers the surface of the implant. Strata to reach from the bones of the lower jaw to the center of the implant. Composite bone-ceramic implant with a modified platelet-rich plasma, extracted from the mandible experimental animal after 30 days after implantation is shown in Fig.13 and Fig.14. Newly formed bone is formed directly on the implant surface. A large array of fabrics dyed represents the deposition of the newly formed bone. Observed the formation of osteoid on the implant surface and the area of the bone-implant. The formation comes from the bone bed to the center of the implant. On the periphery of the implant structure of bone is identical to the intact bone of the jaw, as the distance from the bone bed structure of the newly formed bone unstructured, which is typical for the stage of formation of osteoid.
Thus, the results of experimental studies have shown that the inflammatory reaction caused by the implanted materials in any series of the experiment, was not observed. Bone formation is by layering, in some areas, the mass of newly formed bone on the implant is large, but always there is a connection with the mater is nskoi bone. Proliferation and migration of bone cells leads to appositional bone growth on the implant surface. In all periods of the experiment, there is a pronounced mineralization of the extracellular matrix calcium ions. When applying the modified platelet-rich plasma is the activation of both stromal and hematopoietic component of bone formation (developing the stroma of the bone marrow and are hematopoietic cells). In late period (30 days) use of a modified platelet-rich plasma to remove the implant from the bone of the jaw was not possible due to immured implant newly formed bone tissue. In late period (30 days) is showing signs of biodegradation of the material of the implant, more pronounced in the case of the use of platelet-rich plasma. Resorption of the material of the implant occurs without apparent involvement of histiocytes and osteoclasts.
The study results of the claimed invention demonstrate the ability to effectively use in medical practice for repair of damaged bone structure as a composite bone-ceramic implant-based ceramic material system Zirconia - alumina optimizes the process of repair and recovery, damaged the th bone structure, fast healing and restoration of bone structure. Increases biocompatibility and mechanical properties, ensures the absence of immune response, enhances the reparative processes in the damaged tissues.
Research results indicate that composite bone-ceramic implant-based ceramic material system Zirconia - alumina, effective and useful in medicine to restore anatomic integrity and function of bone tissue.
The invention can be manufactured using hi-tech equipment, modern technologies and materials.
1. Pittenger, M. F., Mosca, J. D., McIntosh K. R., Human mesenhymal stem cells: progenitor cells for cartilage, bone, fat and stroma. Curr Top Environ Immunol. 2000; 251:3-11.
2. Salgado A. J., Oliveira J. T., Pedro A. J., R. L. Reis Adult stem cells in bone and cartilage tissue engineering. Curr. Stem Cell. Res. http://Ther.2006.sep; 1(3):345-64.
3. Liu W., Cui L., Cao Y. Mesenhymal stem cells and tissue engineering. Methods in enzimology. 2006. Vol. 420. P. 339-361.
4. RF patent № 2309756 from 10.11.2007.
5. Yao J., Radin, S., Leboy p, Ducheyne P. The effect of bioactive glass content on synthesis and bioactivity of composite poly (lactic-co-glycolic acid)/ bioactive glass substrate for tissue engineering. Biomaterials. 2005 May; 26(14):1935-43.
6. Bostman O., Paivarinta U., Partio e, Vasenius j, Manninen m, Rokkanen P. Degradation and tissue replacement of an absorbable polyglycolide screwin the fixation of rabbit femoral osteotomies. J. Bone Joint. Surg. Am. 1992. Aug; 74(4): 1021-31.
7. RF application No. 2000109319 from 20.02.2003.
HDMI is traditional bone-ceramic implant, containing porous ceramic media based on Zirconia - alumina, characterized in that the carrier is coated with the layer of hydroxyapatite and platelet-rich plasma, while the carrier is obtained by preparing a mixture of ceramic powder based on ZrO2(Mg, Y) or Al2About3and additives plasticizer and pore-forming Al(OH)3or Zr(OH)4followed by adding distilled water to give a mixture of molding properties and sintering the finished product.
FIELD: medicine, pharmaceutics.
SUBSTANCE: group of inventions refers to medicine, more specifically to absorbable polyether esters that have been found to reduce bacterial adhesion to materials such as medical devices and implants. Amorphous copolymers are used to produce a coating for medical devices and implants to reduce bacterial adhesion.
EFFECT: invention refers to new amorphous copolymers containing polyethylene diglycolate (PEDG) copolymerised with lactide-rich monomers.
15 cl, 4 dwg, 7 tbl, 13 ex
SUBSTANCE: there are described new reinforced biodegradable frames for soft tissue regeneration; there are also described methods for living tissue support, extension and regeneration, wherein the reinforced biodegradable frame is applied for relieving the symptoms requiring high durability and stability apart from patient's soft tissue regeneration. What is described is using the frames together with cells or tissue explants for soft tissue regeneration in treating medical prolapsed, e.g. rectal or pelvic prolapse, or hernia.
EFFECT: frames are adequately durable to be applicable for implantation accompanying the medical conditions requiring the structural support of the injured tissues.
14 cl, 19 dwg, 2 tbl, 8 ex
SUBSTANCE: tissue regeneration or healing is stimulated when using a structure comprising a multilayer plate of a collagen membrane material, which contains a lamellated barrier material of pure collagen prepared of a natural collagen tissue; the lamellated barrier material containing a barrier layer with an outer smooth barrier surface and a fibre surface, which is opposite the outer smooth barrier surface. The structure additionally contains a matrix layer of a collagen sponge material adjoining the fibre surface.
EFFECT: matrix layer of the collagen sponge material is absorbed by an individual's body at a higher rate, than the lamellated barrier material.
20 cl, 3 dwg, 5 ex
SUBSTANCE: invention refers to medicine. What is described is a method for measuring antimicrobial-coated tubular polyurethane products, including multiple-lumen polyurethane catheters consisting in the three-staged chlorhexidine and/or its salts modification. Chlorhexidine and/or its salts are impregnated on the product surface by processing the products in aqueous-alcohol solutions of chlorhexidine and/or its salts, removing excessive chlorhexidine and/or its salts from the product surface, applying a solution of polyurethane in tetrahydrofurane containing chlorhexidine and/or its salts, and evaporating tetrahydrofurane.
EFFECT: preparing the polyurethane products, eg catheters possessing the prolonged antimicrobial activity being in full accord with the multifunctionality of the multiple-lumen catheters.
9 cl, 1 tbl
SUBSTANCE: antimicrobial composition for coating a medical device includes a material, which forms a polymer film, and an antimicrobial preparation from the traditional Chinese medicine, selected from a group: extract of Houttuynia cordata, sodium houttuyfonat and sodium new houttuyfonat or their mixtures. The medical device, covered with an antimicrobial composition, is made in the form of an implanted device.
EFFECT: invention provides the antimicrobial effectiveness with respect to microorganisms - causative agents of surgical infections.
19 cl, 2 dwg, 7 tbl, 4 ex
SUBSTANCE: invention refers to medicine. What is described is a bioresorbable hydrogel polymer composition for cardiovascular surgery in the form of a film prepared by a reaction of natural polymers, biologically active substances, a solvent and a softening agent wherein the polymers are presented by cross-linked bioresorbable polymers - gelatin, chitosan or a mixture of chitosan and gelatin, chitosan and polyhydroxybutyrate; the biologically active substance or mixtures thereof are presented by the antioxidant L-carnosine, the anticoagulant heparin, the antiaggregant dipyridamole, acetylsalicylic acid, the non-steroid anti-inflammatory preparation acetylsalicylic acid, the antimicrobial preparations - ciprofloxacin, metronidazole; mechanical strength of the film is not less than 1.2 MPa, the relative elongation is no more than 160%, and the elasticity modulus is 0.4-5 MPa.
EFFECT: there are used hydrogel polymer compositions with the control bioresorption period, prolonged length of biologically active substance release, having biocompatible and thrombus-resistant properties and improved mechanical characteristics - higher softness and elasticity.
7 cl, 12 dwg, 2 tbl, 4 ex
SUBSTANCE: invention refers to medicine and tissue engineering, and may be used in cardiovascular surgery for small-vessel bypasses. A vascular graft is made by two-phase electrospinning with the staged introduction of the ingredients into the polymer composition.
EFFECT: making the bioresorbed small-diameter vascular graft possessing the improved biocompatibility ensured by using the polymer composition of polyhydroxybutyrate (PHBV) with oxyvalerate, and epsilon-polycaprolactone with type IV collagen, human fibronectin and human fibroblast growth factor (hFGF) additionally introduced into the composition.
2 cl, 1 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine. What is described is a biomaterial on the basis of calcium phosphate, preferentially on the basis of hydroxyapatite, or on the basis of a material containing hydroxyapatite, such as diphase calcium phosphates and calcium phosphate cements, and using it for making an implant or for positioning prosthesis for the purpose of osteoanagenesis.
EFFECT: biomaterial provides the excellent properties of biological compatibility and fast osteoneogenesis.
17 cl, 4 dwg, 1 tbl
SUBSTANCE: invention relates to chemical-pharmaceutical industry and represents artificial dura mater, produced from electrospinning layers by technology of electorspinning, with electrospinning layer, consisting of, at least, hydrophobic electrospining layer, which is produced from one or several hydrophobic polymers, selected from polylatic acid and polycaprolactone.
EFFECT: invention ensures creation of artificial dura mater, which has good tissue compatibility, anti-adhesiveness and possibility of introducing medications, preventing cerebrospinal fluid outflow during regeneration of person's own dura mater.
30 cl, 7 ex, 11 dwg
SUBSTANCE: claimed invention relates to ophthalmological device, method of its obtaining. Device contains antimicrobial particles of metal salts, which have size less than approximately 200 nm, dispersed throughout polymer mass. Device ensures at least 0.5 log reduction of at least one of Pseudomonas aeruginosa and S.aureus, and opacity value constituting less than 100%, with 70 micron thickness, in comparison with CSI lens.
EFFECT: invention possesses high antibacterial activity 3 independent claims, 34 dependent claims of invention formula.
17 tbl, 46 ex, 5 dwg
SUBSTANCE: what is described is a method for impregnation of porous polymer matrixes with liquid slurry of calcium carbonate powder containing sintering additives of alkaline metal carbonate or carbonates. The taken samples are dried and thermally treated to remove a fluid and to decompose an organic polymer. That results in producing porous ceramic matrix of calcium carbonate having a porosity of 40-60% and a compression resistance of 4-10 MPa after final sintering at temperature 540-620°C. What is described is a calcium carbonate material possessing the bioadhesive properties and high bioresorption that promotes the fast bone tissue repair.
EFFECT: calcium carbonate material possessing the bioadhesive properties and high bioresorption, promotes the fast bone tissue repair.
SUBSTANCE: method includes obtaining a charge for sintering ceramic materials from a powder, synthesised by precipitation of salts in water solutions, with a component ratio: 60-95 wt % of calcium carbonate and 5-40 hydroxyapatite and/or carbonate hydroxyapatite. The claimed method also includes additional milling of the obtained powder and introduction into the powder before and after milling of sodium carbonate in an amount of 0.25-5.0 wt %, taken with respect to calcium carbonate and hydroxyapatite and/or carbonate hydroxyapatite.
EFFECT: obtaining a composite ceramic material with higher compression strength and lower porosity.
1 tbl, 4 ex
SUBSTANCE: invention relates to a method of producing canaphite, i.e. a hydrated disodium pyrophosphate of calcium (Na2CaP2O7*4H2O). The method involves feeding starting components: water, a crystalline hydrate of sodium pyrophosphate, calcium acetate or a crystalline hydrate of calcium nitrate, in molar ratio of the crystalline hydrate of sodium pyrophosphate to calcium acetate or crystalline hydrate of calcium nitrate of 0.98-1.02 and ratio of the mass of water to mass of the produced canaphite of 0.7-0.8. Said components then react while mixing intensely in a planetary mill at a rate of 5000-7000 rpm for 1-3 hours. The obtained product is then washed with water 4-6 times and disaggregated.
EFFECT: method enables to obtain single-phase canaphite powder with particle size in the range of 100-500 nm with high output of the end product.
1 tbl, 2 ex
SUBSTANCE: invention refers to medicine, and may be used for facialmaxillary osseous prosthesis, as a drug delivery system and as a matrix for tissue engineering structures. Bruschite cement contains (wt %): β-tricalcium phosphate - 34.5-45, monocalcium phosphate monohydrate - 23-29.5, bioactive glass - 0.5-10, water-base tempering fluid 25-32.5. The bioactive glasses are strongly alkaline glasses as follows (wt %): SiO2-40-60, CaO-10-25, Na2O-22-35, P2O5-3-5, of insular, chain, ring or layer silica-oxygen motifs. The glasses are introduced in the amorphous or crystalline state at the granule dispersity 30 to 80 and 80 to 120 mcm.
EFFECT: allows adjusting the dig structure of the material, its mechanical properties, pH and solubility in the buffer solutions, as well as enables intensifying the regenerative processes in the injured osseous structures and reducing the length of treatment and recovery of the bone integrity.
8 cl, 8 ex
FIELD: process engineering.
SUBSTANCE: invention relates to production of beta-tribasic calcium phosphate ceramic articles fur use as bone implants. Proposed method comprises the following steps: preliminary heat treatment of gypsum preforms for 2.0-2.5 hours, conversion of said gypsum preform in hydroxyapatite in 1-molecular solution of ammonium phosphate for, at least, 24 hours and further heat treatment at 970-1030°C.
EFFECT: hardness of, at least, 2,2 MPa.
1 tbl, 2 ex
SUBSTANCE: invention relates to medicine, more specifically to ophthalmology, and is applied for prosthetics of cadaverous cavity of eye socket after enucleation of eye bubble at its application with purpose of keratoplasty. Eye cadaverous prosthesis is ball-shaped, with imitation of sclera and iris. From the back pole of prosthesis diverges sharp flat pin, 20-50 mm long, 7-15 mm wide, 0.5-2.0 mm thick, with notched edges, on the principle of fishhook.
EFFECT: invention ensures full imitation of natural human eye in corpse after eye bubble enucleation, as well as reliable fixation of eye cadaver prosthesis in necessary position.
2 dwg, 2 ex
SUBSTANCE: invention relates to a method of producing porous bioactive glass-ceramic material. Said method involves preparing a semidry mass containing powdered calcium phosphate glass and 1-10% solution of a polymer selected from polyvinyl alcohol, gelatine, methyl cellulose or carboxymethyl cellulose, which is then moulded in paper masters at moulding pressure 1.0-1.2 MPa, dried, burnt and held for 0.5-1.5 hours at 900-1000°C. Main channel foaming agents in form of caprone, nylon, lavsan or graphite in amount of 3-30 wt % and starch in amount of 1-10 wt % are then added to the semidry mass. The calcium phosphate glass has the following composition in mol %: CaO - 40-55, Al2O3 - 4-10, B2O3 - 1-5, TiO2 - 1-10, ZrO2 - 1-10 and P2O5 - the balance.
EFFECT: method enables to obtain bioactive glass-ceramic materials for implantation with a channel and an inter-channel pore structure with open porosity of 20-70%, content of pore channels of 5-40%, channel pore diameter of 100-700 mcm, pore size in inter-channel partitions of 20-100 mcm.
2 cl, 8 ex
SUBSTANCE: invention refers to a bioactive microcellular material for orthopaedic surgery, which contains certain proportions of isle-, chain-, ring- and layer-pattern powdered strongly alkaline glass of certain composition, powdered calcium phosphate excipient, hydroxyapatite specified in calcium-deficient hydroxyapatite with the Ca/P ratio = 1.5-1.65 or β-tricalcium phosphate, and a cellulating agent representing starch or gelatine. Besides, the invention refers to a method for making said material applied for the purpose of orthopaedic surgery which involves the stages of material granulation, moulding and heat treatment. At the stage of granulation, glass of dispersity 40-120 mcm is introduced into the powdered material, while the heat treatment is three-staged: at first the material is heated up to 600-750°C and kept for 30-50 minutes, then tempered to 520-550°C and kept for 5-10 minutes and cooled slowly to 450-470°C and kept for 5-10 minutes.
EFFECT: making the materials characterised by adjustable solubility, high hydrophilic properties, biomodal pore structure and high mechanical properties.
2 cl, 4 ex
SUBSTANCE: method to prepare charge to produce bioceramics includes dosing of initial components and their grinding in a planetary mill. Initial components are hydroxyapatite and sodium dihydrophosphate at the mass ratio of "hydroxyapatite/sodium dihydrophosphate" in the range of 20/80 - 85/15.
EFFECT: method makes it possible to prepare charge for bioceramics of new generation, capable of resorption and stimulating new bone tissue.
1 tbl, 1 ex
SUBSTANCE: invention refers to medicine and represents a biotransplant based on a high-porous ceramic material of zirconium oxide - aluminium oxide system and multipotent stromal cells of human bone marrow for restoring the extended bone tissue defects characterised by that contains autologous or donor multipotent mesenchimal stromal cells (MMSC) of bone marrow and a carrier made of the high-porous ceramic material of zirconium oxide - aluminium oxide system by polyurethane foam base doubling with the carrier being densely seeded with MMSC cultivated within 1 to 3 passages; one carrier contains 200 to 500 thousand cells immobilised of the vitality at least 90%, while the functional targeting is verified by the ability to the directed differentiation of the mesodermal lines and the expression of stromal markers CD90 and CD105 in 60 - 90% of cells and the absence of expression of marker CD34 is shown.
EFFECT: invention provides accelerated repair processes and more effective osteointegration.
SUBSTANCE: described is method of obtaining oxide biocompatible coatings on steel transosseous implants, which is realised by their thermal oxidation in air at temperature 300-600°C under conditions of blowing by air, supplied into working chamber of furnace under pressure 1.5-1.7 atm for 0.1-0.2 h, with further cooling of oxidised implants in furnace with switched off heating to the temperature of the environment.
EFFECT: method makes it possible to obtain biocompatible oxide coating on steel transosseous implants and possesses higher technical-economical efficiency, consisting in reduction of oxidation process duration and accelerated formation of oxide coating.