Products and method of decontaminating prions
SUBSTANCE: group of inventions relates to a method of decontaminating products or materials infected with prions, and is intended for decontaminating medical or medical surgical devices at risk, decontaminating work surfaces, decontaminating animal meal or other contaminated products of animal origin. The step for decontaminating products or materials infected with pathogenic agents responsible for prion diseases comprises placing them in contact with a solution containing copper (Cu) and/or derivatives thereof. Said copper and/or derivatives thereof are present in an amount of at least 500 mcM.
EFFECT: use of the group of inventions improves efficiency of decontaminating infected products or materials.
7 cl, 4 ex
The object of the present invention are products and methods for disinfecting products and materials infected with non-standard vector-borne pathogens (ATNC) responsible for transmissible subacute spongiform encephalopathy.
The invention concerns, in particular, disinfection products and materials infected with prions, which accumulate mainly in the brain of the host, in particular in the form of abnormal isoforms of PrPSC(PrPscrapie), which is the result of a conformational change of the protein PrPC(cellular PrP), encoded by the owner.
The emergence of a new variant of the disease of Creutzfeldt-Jakob disease in the UK and its possible relationship with spongiform encephalopathy of cattle were brought to the fore the potential transmissibility of the disease, in particular, through an infected animal products, in particular, through contaminated food and medical-surgical supplies.
Indeed, modern methods of disinfection and sanitation cannot easily destroy prions, which are resistant to most commonly used methods. Administrative Guidelines prescribed rules that must be followed in this matter, but they are very difficult to implement, are expensive and require the use of toxic products.
In light of this the th context, the authors conducted a search of compounds, which would simply and efficiently to carry out the clearance of the pathogenic agent and which could be used on the vast majority of medical-surgical surfaces and materials. Their work showed the effectiveness in this regard, some metal derivatives.
The invention aims, therefore, the use of such compounds for disinfection of prion. It is also concerned with a method of handling infected materials and products, including the use of these compounds.
The invention relates, therefore, application of Cu and its derivatives for the treatment of prion.
According to the supplementary regulations of the invention, these derivatives are used in conjunction with H2O2.
Preferably the metal is derived CuSO4. One or more compounds used according to the invention, are mostly in the form of aqueous solutions.
Studying the effects of these derivatives on the clearance of pathogenic agents responsible for prion diseases, showed their high efficiency for purification of contaminated foods or materials.
Preferably these compounds are non-toxic, biodegradable, easy to use.
The invention relates thus also to the way abyssaria the Oia products or materials infected with pathogenic agents responsible for prion diseases, characterized in that it comprises bringing them into contact with at least one compound, as defined above, or its containing solution, or, if necessary, with H2O2.
In a preferred method of carrying out the invention a derivative of metal is CuSO4.
Disinfecting treatment is carried out mainly with the use of a solution containing the specified connection is based at least 500 μmol, in particular from 500 to 1000 µmol.
H2O2when it is used, is present in the solution at a ratio of approximately 50 mmol.
Satisfactory results were obtained by exposure at ambient temperature for about 15-60 minutes, in particular for approximately 30 minutes
This or these compounds and their solutions are, therefore, of great interest within the hospital, where they allow, in particular, to produce disinfection of medical or medical-surgical devices-risk groups, such as materials reuse, such as endoscopes, probes (dialysis), as well as decontamination of work surfaces such as laboratory table or the floor, also included in the risk zones.
These connections or irectory also especially useful for disinfecting, for example, infectious material of cerebral origin and biological products, derived from carriers of infectious forms of the disease of Creutzfeldt-Jakob disease. The invention also concerns the use of these compounds and their solutions for cleaning products blood origin or biological material used in transplants.
Preferably these compounds and solutions have proven effective on the prion strain associated with ESB (transmissible spongiform encephalopathy of cattle, or mad cow disease). They are applicable, therefore, to any type of prion whatever its strain and origin of the pathogen.
They can also successfully be used in food applications, in particular, to neutralize any potentially infectious compounds and, in particular, animal flour or other products derived from infected animals.
Worthy of interest applications include disinfection of areas such as abattoirs, surfaces and devices at risk of becoming in contact with infectious agents. Can be called, for example, especially those who come from ruminant animals.
Other characteristics and advantages of the invention are given in the following examples, in which reference is made to Fig. 1 and 2, which represent, with the responsibility.
Example 1. The solution for disinfection of prion-containing CuSO4and H2O2
a) study of the effects of copper together with H2O2the destruction of PrPSCpresent in the homogenates of infected mouse brain.
Solutions CuSO4and H2O2in various concentrations were added to the samples, consisting of extracts from infected homogenate of rat brain, and left in contact for approximately 30 minutes at ambient temperature. Then the samples were placed on a gel of polyacrylamide SDS-PAGE after equalization of the concentrations of proteins and decomposition with proteinase K (PK) at a concentration of 1 mg PK 50 mg of protein. The presence of PrPSCdetected using Western blotting.
The obtained results are illustrated in Fig. 1. It is seen that at a concentration of 100 µmol CuSO4and 50 mmol of H2O2infected samples have decreased levels of PrPSC. At a concentration of 500 μmol CuSO4and 50 mmol of H2O2the content of PrPSCpresent in homogenates of infected, is not detectable by Western blot (lanes 7 and 8). At this concentration effect is enhanced by the action of H2O2.
When you use the higher doses CuSO4from 1 mmol to 10 mmol only one copper eliminates signal PrPSCand on babok H 2O2is no longer required.
These results were confirmed by repeating these tests in homogenates derived from the brain of mice infected with strain 22L prion. Similar results were obtained with homogenates derived from mouse strain Chandler and ESB, which suggests that the disinfectant effect is not dependent on the strain.
b) study of the infectivity of homogenates of infectious brain processed solutions with a high concentration of copper in vitro.
Homogenates of brain 22L were treated for about 30 min with different concentrations of solutions CuSO4associated or not with H2O2in various concentrations. These infectious homogenates were subjected to dialysis, then was placed on cells of mouse neuroblastoma having the ability to fight the pathogen.
Homogenates of untreated brain were used as the control.
Western blotting was carried out after 6 passages in cell lysates and after decomposition with PK in order to detect the presence of PrPSCmeaning that the tested samples are still infectious.
The results are shown in Fig. 2. It can be stated that the infection is reduced as a result of processing.
Example 2. The in vivo tests
It was confirmed results observed in vitro. The brain homogenates infected with 22L, were treated, in particular, a solution of 500 µmol CuSO4and 100 mmol H2O2. The homogenates treated or not, were inoculated mice intracerebrally way. Control animals infected untreated homogenates, all sick for 168 days +/-2 days. Some animals infected-treated homogenates were alive even after more than 300 days. This result proves that the reduction of the titer of infection for at least 7 orders of magnitude can be obtained by this method of disinfection.
Example 3. Decontamination of surgical materials
Such materials, such as endoscopes, immersed in a solution containing l mg CuSO4on 20 minutes the Tests performed to identify the presence of prion after this treatment turned out to be negative.
Example 4. The effectiveness of disinfection
The following are data regarding the effectiveness of disinfection tested in vivo after vaccination in mice. For this control and disinfected the brain homogenates were inoculated intracerebrally way mice (C57B1).
The survival time of mice has the following values:
1) Inoculation control of infectious homogenates: 167,6 +/-0,5 days.
2) the Homogenates treated in CuSO4(0.5 mmol, 30 min, ambient temperature): 200,2 +/8.9bn days.
3) Homoge the ATA, processed in CuSO4(1 mmol, 30 min, ambient temperature): 217,2 +/-13,4 days.
4) Homogenates treated in CuSO4(1 mmol) + H2O2(100 mmol, 30 min, ambient temperature): 266,4 +/-15,6 days.
Taking into account such terms incubation periods in these different conditions and curve of the titration of the pathogen collected from these mice, it is possible to estimate that the achieved disinfection exceed 104in homogenates treated only CuSO4and above 105in homogenates treated in CuSO4+ H2O2.
1. The method of disinfection products or materials infected with pathogenic agents responsible for prion diseases, characterized in that stage decontamination is their introduction to the contact:
the solution containing copper (Cu) and/or its derivatives, and the copper and/or its derivatives are present in amounts of at least 500 microns.
2. The method according to p. 1, characterized in that the Cu and/or its derivatives are present in amounts of from 500 to 1000 microns.
3. The method according to p. 1, characterized in that the Cu and/or its derivatives are used in conjunction with H2O2.
4. The method according to p. 3, characterized in that the H2O2is present in the amount of approximately 50 mm.
5. The method according to p. 1, characterized in that a derivative of copper is CuSO .
6. Application of the method according to one of paragraphs.1-5 for disinfection of medical or medical-surgical devices-risk groups, such as materials reuse, such as endoscopes, or for decontamination of work surfaces such as laboratory table or the floor.
7. Application of the method according to one of paragraphs.1-5 for disinfection of animal flour or other contaminated foods of animal origin.
SUBSTANCE: invention relates to active compositions of a fabric softener. Described is a composition of the fabric softener, containing from 80 to 95 wt % of bis-(2-hydroxyethyl)dimethylammonium chloride ether of fatty acid, which has a ratio of quantity of moles of fatty acid fragments to the quantity of moles of amine fragments, equal from 1.80 to 1.96, an average length of a chain of fatty acid fragments constituting from 16 to 18 carbon atoms and a iodine number, equal from 0 to 50, from 2 to 8 wt % of fatty acid triglyceride, which has an average length of fatty acid fragments constituting from 10 to 14 carbon atoms, and a iodine number equal from 0 to 15, and from 3 to 12 wt % of alcohol, selected from a group, including ethanol, 1-propanol and 2-propanol. Also claimed is a method of obtaining the fabric softener composition.
EFFECT: low viscosity of melt, high stability in a melted state and bad inflammability.
8 cl, 1 tbl
SUBSTANCE: invention relates to active fabric softener compositions. Described is an active fabric softener composition which contains a) not less than 50 wt % bis-(2-hydroxypropyl)-dimethylammonium methylsulphate ester of a fatty acid, having molar ratio of fatty acid moieties to amine moieties ranging from 1.5 to 1.99, average chain length of fatty acid moieties ranging from 16 to 18 carbon atoms, and iodine number of fatty acid moieties calculated for a free fatty acid ranging from 0.5 to 50, and b) 0.5-5 wt % fatty acid. Also described is a method of producing the fabric softener composition.
EFFECT: good softening capacity, good stability when stored in an aqueous dispersion, the softener composition can be used and processed in a liquid state without adding a flammable solvent.
15 cl, 2 tbl, 19 ex
FIELD: process engineering.
SUBSTANCE: proposed method comprises placing the part into container that sustains a variable pressure. Foam composition is applied to the part including at least one foaming agent, at least one ferment and dissolved gas. Note here that said foam composition is redistributed over the part at application and/or after part cleaning. The part is cleaned by foam composition at application of variable pressure to container. Besides, said part is rinsed with appropriate fluid at pressure application to said container. Note here that pressure in said container is increased unless said foam composition behaves as a fluid.
EFFECT: better cleaning.
14 cl, 6 dwg, 5 tbl
SUBSTANCE: invention relates to methods of producing a fabric softener. Disclosed are methods of producing a fabric softener composition, containing 1-49% bis-(2-hydroxyethyl)-diethylammonium chloride of a fatty acid ester by weight of the composition, which include a step of mixing water with an active composition (FSAC), wherein the FSAC contains 65-95% by weight of FSAC of bis-(2-hydroxyethyl)-diethylammonium chloride of a fatty acid ester, having molar ratio of fatty acid fragments and amine fragments of 1.80-1.96, average chain length of fatty acid fragments of 16-18 carbon atoms and iodine number, calculated for a free fatty acid, of 0-50, 2-8% by weight of FSAC of a fatty acid triglyceride, having average chain length of fatty acid fragments of 10-14 carbon atoms and iodine number, calculated for a free fatty acid, of 0-15, and 3-12% by weight of FSAC of alcohol, selected from ethanol, 1-propanol and 2-propanol.
EFFECT: preparing a fabric softener composition with low content of flammable solvents, low melt viscosity and high stability in molten state.
22 cl, 1 tbl
SUBSTANCE: invention relates to compositions for controlling foaming for liquid detergent systems. Disclosed is a composition for controlling foaming, which contains: (A) an organosilicon antifoaming agent containing (i) organopolysiloxane having at least one substitute of formula X-Ar linked to silicon, where X is a divalent aliphatic group linked to a silicon atom through a carbon atom, and Ar is an aromatic group, (ii) an organosilicon polymer of formula R1 aSiO(4-a)/2, where R1 is a hydrocarbon group, hydrocarbon oxy or hydroxyl, and a has an average value from 0.5 to 2.4, and (iii) hydrophobic filler, and (B) organopolysiloxane resin containing at least one polyoxyalkylene group, as well as tetrafunctional siloxane links of formula SiO4/2 and monofunctional siloxane links of formula R2 3SiO1/2, wherein the total number of tetrafunctional siloxane links in the resin is not less than 50% of the total number of siloxane links, and R2 is a hydrocarbon group. Also disclosed is a liquid detergent containing one or more surfactants, weater and said composition for controlling foaming.
EFFECT: disclosed composition is safe and inert to detergent components, provides stability of the mixture in detergents and excellently control foaming.
4 cl, 1 dwg, 2 tbl, 8 ex
SUBSTANCE: invention relates to a liquid hand dishwashing detergent composition which contains: (a) 0.001-10 wt % cationic polymer and (b) 0.005-3 wt % active inorganic mother-of-pearl agent having particle size smaller than 50 mcm, and the cationic polymer is a carboxyethyl cellulose salt. The present invention relates to a method of cleaning dishes.
EFFECT: obtaining a dishwasher detergent which cares for skin, particularly correction of cysts and skin lustre.
22 cl, 12 ex
SUBSTANCE: present invention relates to a method of reducing creases when washing a garment made from heavy fabric, which includes steps of washing the garment and applying a fabric softener, which contains a quaternary ammonium fatty ester and a hydrolysed protein and polysiloxane copolymer, where the average molecular weight of the protein component ranges from 500 to 500000 Da, to the garment made from fabric weighing at least 250 g/m2. The present invention also relates to a fabric softener for reducing creases on garments made from heavy fabric, which contains: a) 0.5-50 wt % cationic softener, which contains a fatty acid ester of quaternary ammonium, b) 0.02-0.5 wt % hydrolysed protein and siloxane copolymer, where the average molecular weight of the protein component ranges from 500 to 500000 Da, c) a fragrant and d) water.
EFFECT: obtaining an efficient and cheap agent for removing or reducing creases on clothes during washing and drying.
13 cl, 3 ex, 4 tbl
SUBSTANCE: foaming composition contains (i) at least one component of formula (1)
where one of symbols B1, B2, B3 independently denotes an acyl group represented by -CO-R and the rest denote H, (ii) at least one component of formula (1), where two of symbols B1, B2, B3 independently denote an acyl group represented by -CO-R and the rest denote H, (iii) at least one component of formula (1), where each of symbols B1, B2, B3 independently denotes an acyl group represented by -CO-R, (iv) at least one component of formula (1), where each of B1, B2 and B3 denotes H. The weight ratio of components (i)/(ii)/(iii) is equal to 60-90/10-35/less than 10 in formula (1), where R' denotes H or CH3, each of m, n or l independently denotes a number from 0 to less than 9, where the sum of m, n and l ranges from more than 5 to less than 9, where in the acyl group represented by -CO-R, R denotes an alkyl or alkenyl group, linear or branched, containing 6 to 9 carbon atoms, and the weight ratio of (i)+(ii)+(iii)/(iv) ranges from 2.0:0.5 to 0.5:3.0. The invention also relates to use of the foaming composition as a foaming enhancing or stimulating agent in detergent compositions.
EFFECT: foaming composition enables to achieve maximum foam volume and increase the number of theoretical plates.
5 cl, 5 tbl, 3 ex
SUBSTANCE: alkaline detergent for fabric hand washing contains a foaming surfactant, polyethylene imine defoamer having empirical formula (PEI)aEO)b(PO)c (I), where a assumes a value from 100 to 100000, b assumes a value from 5 to 60 and c assumes a value from 2 to 60; and a pH control system. When said detergent is diluted with water in weight ratio 1:350 in order to prepare a washing solution and during washing, the pH control system keeps pH of the washing solution at temperature 20°C higher than 8. The present invention relates to a method for hand washing fabric, involving steps for providing the laundry detergent, preparing the washing solution by diluting the detergent with water in weight ratio from 1:150 to 1:1000, keeping pH of the washing solution higher than 9, hand washing the fabric in the washing solution, keeping pH of the washing solution between 9 and 13 during the washing step, rinsing the linen in a rinsing bath, wherein pH of the rinsing bath is lower than 9. The invention also relates to a method of saving water, involving a step for washing fabric in accordance with the hand washing method.
EFFECT: reduced foaming after the first rinsing.
12 cl, 4 ex, 1 tbl
SUBSTANCE: invention describes fabric softening compositions containing approximately from 0.01 to 50% cationic or nonionic softening compound; approximately from 0.01 to 5% fragrance component; and at least 0.001 wt % polymer material capable of holding ingredients of a volatile fragrance component, containing: at least approximately 0.001 wt % cross-linked polymer containing at least one vinyl monomer; and approximately from 5000 to 100000 ppm divinyl cross-linking agent. Also disclosed is a fabric softening method which involves bringing fabric into contact with the composition of a fabric softening agent in an effective amount, where contact takes place by sprinkling, rubbing or rinsing.
EFFECT: improved composition properties.
26 cl, 5 ex, 4 tbl
SUBSTANCE: method for making a plate from modified xenogeneic enteric submucosa involves mechanical evacuation of small bowel, processing in hypertonic saline in a combination with ultrasonic exposure, enzymatic treatment, subsequent washing in acetic acid and sodium hydrocarbonate, sodium chloride processing, additional ultrasonic exposure and enzymatic treatment, storage in acetic acid and sodium hydrocarbonate, storage in an antimicrobial agent and multiply changed ascending glutaric dialdehyde. The biotissue is stored in multiply changed ascending glutaric dialdehyde in the melted form between the two plates of porous non-woven material.
EFFECT: preparing the non-immunogenic biomaterial uniformly cross linked throughout with particularly damaged collagen structure that provides the fast recipient's cell penetration into the transplant and smooth invasion of vessels and tissues in the postoperative period.
SUBSTANCE: what is described is a composition for an antimicrobial coating containing inorganic substance nanoparticles, an active substance, a binding agent and a solvent. As the inorganic substance, the composition contains silicone dioxide; the active substance is presented by a mixture of quaternary ammonium compound and chlorhexidine; the solvent is a mixture of ethyl cellosolve and butyl cellosolve; the binding agent is a mixture of polymethylphenylsiloxane resin and butyl/methyl methacrylate copolymer in the following proportions, wt %: butyl/methyl methacrylate copolymer 1.70-10.0, polymethylphenylsiloxane resin 5.0-20.0, silicone dioxide 0.5-3.0, chlorhexidine (20% aqueous) 3.0-8.0, quaternary ammonium compound 0.5-3.0, ethyl cellosolve 20.0-50.0, butyl cellosolve to 100%.
EFFECT: providing the water-stability of the long-action antimicrobial coating, ecological safety and applicability for porous surface finish.
4 cl, 5 tbl, 1 ex
SUBSTANCE: set task is solved by application of final slag, formed in production of ferrovanadium by alumino-silicothermic method as bactericidal material.
EFFECT: extension of raw material resources for bactericidal materials.
SUBSTANCE: method includes forming, on a surface, a coating from a dimethylalkylbenzylammonium chloride complex containing a C10-C14 alkyl group or a mixture of C8-C16 alkyl groups with a copolymer of styrene and maleic anhydride, which is subjected to preliminary chemical modification to open anhydride rings to form carboxyl groups, 50-60% of which are neutralised to form carboxylate groups, or a mixture of said complex with polyvinyl butyral, in the following ratio, wt %: antiseptic polymer complex 25-40, polyvinyl butyral 60-75.
EFFECT: full prolonged protection of articles made of aluminium alloy from fouling by microorganisms, which prevents corrosive change of the surface of said alloys.
28 ex, 3 tbl
SUBSTANCE: invention relates to means of purifying and disinfecting different types of water. Described is a disinfectant which contains a first polyhexamethylene guanidine hydrochloride component, a second alkyldimethylbenzylammonium chloride component and water, wherein content of the components in the solution is selected in the following amounts, wt %: first component 7-34.5, second component 1.75-6.9, water - the balance.
EFFECT: disinfection of drinking water.
SUBSTANCE: group of invention relates to a synergistic antimicrobial composition which includes a zinc salt of glyphosate and 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one, and is intended for controlling the level of fungi and algae. The group of inventions also provides a method of inhibiting growth or controlling growth of microorganisms in building material by adding said synergistic antimicrobial composition, a coating composition containing said synergistic antimicrobial composition and a dry film made from said coating composition.
EFFECT: use of the group of inventions increases efficiency of inhibiting growth of microorganisms, provides more precise control of the growth of microorganisms without affecting health and/or the environment.
12 cl, 7 tbl
SUBSTANCE: tableted antimicrobial composition contains polyhexamethylene guanidine hydrochloride (PHMH) in a combination with at least one ingredient specified in a group: quaternary ammonium compound (QAC), N,N-bis-(3-aminopropyl)dodecylamine and persol, as well as with an alkaline metal iodide and ammonium iodide. The antimicrobial composition has the following ratio of ingredients: PHMH - 10 to 70 wt %; QAC, N,N-bis(3-aminopropyl)dodecylamine and/or persol - 1 to 30 wt %; an alkaline metal iodide and ammonium iodide - 0.1 to 20 wt %.
EFFECT: using the invention provides the fast-acting tableted antimicrobial composition having a wide antimicrobial spectrum and a higher level of biocidal activity.
5 cl, 2 tbl, 2 ex
SUBSTANCE: invention relates to the field of poultry industry and is intended for sanation of poultry hatching eggs. The method comprises processing the egg surface with aqueous solution of preparation based on milk whey with lactulose and quaternary ammonium compound trimethyloctadecyl ammonium bromide. As the aqueous solution of the preparation based on milk whey with lactulose and quaternary ammonium compound trimethyloctadecyl ammonium bromide 0.1-0.2% aqueous solution of the preparation is used, taken in equal proportions and diluted with water in a ratio of 1:10. Hatching eggs are processed 2-4 hours prior to their laying for incubation.
EFFECT: use of claimed method enables to carry out effectively sanation of poultry hatching eggs.
1 tbl, 1 ex
SUBSTANCE: agent comprising ethanol, glycerol, Carbopol ultrese and softened water according to the invention comprises aminomethylpropanol, essential oil selected from the group: eucalyptus essential oil, lemon essential oil, peppermint essential oil, anise essential oil, jasmine essential oil with the following ratio of components in g per 100 g of gel: ethanol 65.0-66.0; glycerol 1.0-2.0; Carbopol ultrese 0.16-0.18; aminomethylpropanol 0.06-0.08; essential oil 0.005-0.03; softened water - up to 100.0.
EFFECT: invention has a high antimicrobial activity against pathogenic bacteria, fungi and viruses and provides safety for life and health of people without causing allergy.
4 cl, 4 ex
SUBSTANCE: biocidal composition for soaking napkins contains benzethonium chloride, sodium hydroxymethylglycinate, glycerine, decyl polyglucose, niacinamide, liposentol hydro, phenylmethanol and distilled water. Components are used in a defined ratio given in wt %.
EFFECT: invention improves biocidal properties of the napkin soaking composition.
3 tbl, 1 ex
FIELD: veterinary sanitation.
SUBSTANCE: in the suggested disinfectant one should dissolve electrochemically activated neutral anolyte, containing 0.6 mg/ml active chlorine, with running water at 1:5 ratio and the composition of diluted electrochemically activated neutral anolyte should be supplemented with 1-1.5 mg acetic acid per 1 ml preparation. The innovation enables to decrease the concentration of active chlorine and energy expenses at keeping initial high bactericidal activity of electrochemically activated neutral anolyte and, also, apply it in aerosol form for air sanitation in poultry-breeding housings when poultry is present.
EFFECT: higher efficiency of disinfection.