Stimulation method of growth of agricultural plants |
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IPC classes for russian patent Stimulation method of growth of agricultural plants (RU 2542484):
 Strain of bacteria desulfovibrio sp. for wastewater treatment from heavy metal ions / 2542402
Strain of bacteria Desulfovibrio sp. A 4/1 is deposited in the Russian Collection of Microorganisms (RCM) under the registration number of RCM B-2820D, and is resistant to heavy metal ions.
Nutrient medium for determining antibiotic susceptibility of legionella pneumophila / 2542390
Nutrient medium comprises enzyme-autolyzate of cattle spleen, monosubstituted potassium phosphate, disubstituted potassium phosphate, potassium gluconate, mesoinosite, soluble starch, gelatin, L-cysteine, iron (III) sodium salt, high-grade ink and distilled water in a predetermined ratio of components.
 Bacteria bacillus subtilis producing 5`-aminoimidazole-4-carboxamideriboside (aicar), and method of microbiological synthesis of aicar by culturing this bacterium / 2542387
Bacterium Bacillus subtilis is proposed, producing 5′-aminoimidazole-4-carboxamideriboside (AICAR). The said bacterium comprises deregulated pur-operon against the background of inactivated gene purH, the modified heterologous genes prs and purF E.coli under the control of a strong promoter PrpsF as part of chromosome. At that it has one of the following characteristics: comprises the gene zwf under control of a strong promoter PrpsF, comprises the heterologous gene udhA under the control of a strong promoter PrpsF, comprises the deletion of the gene sacB. The method of synthesizing AICAR by culturing the said bacteria under proper conditions is also proposed. At that culturing is carried out on the medium of the following composition, wt %: fodder yeast 0.5-1.0, sucrose 10-13, soy isolate 2.5-3.5, corn-steep extract 3.0-5.0, urea 0,6-0,8, (H34)23CO4 0.8-1.6, propinol 0.4-0.5, water - the rest.
 Using bottom seawater of hydrogen sulphide pool of black sea as sea algae culture medium / 2541448
Invention "Using bottom sea water of hydrogen sulphide pool of the Black Sea as sea algae culture medium" refers to marine culture. Technical substance of the invention consists in using bottom seawater of the Black sea both containing hydrogen sulphide, and oxidised as a sea algae culture medium. Studying the biogenic properties of the aqueous medium from a reduction zone of the Black sea implemented by the invention has shown that the bottom seawater has no bad influence on the Black Sea plankton algae in the presence of high initial concentrations of hydrogen sulphide. After complete oxidation of this xenobiotic, the Black Sea bottom water can be used as a nutrient fertile culture medium of unicellular plankton and multi-cellular benthoalgae in laboratory or industrial environment, and in marine culture farms.
 Saccharomyces cerevisiae vkpm y-3973 yeast strain for fruit-and-berry wine production / 2540023
Invention relates to winemaking industry. "Quince -D" Saccharomyces cerevisiae yeast strain is deposited in the All-Russian Collection of Industrial Microorganisms (VKPM), Federal State Unitary Enterprise State Research Institute for Genetics, under Registration No Y-3973. Y-3973 strain has spore formation capability, ferments and digests glucose, sucrose, maltose, galactose, 1/3 of raffinose. Saccharomyces cerevisiae VKPM Y-3973 yeast strain has a high fermentative activity, well ferments quince wort containing sugar in an amount of 14.75 g/100 cm3 accumulating 8.79 % vol. of ethanol. The strain can more completely digest carbohydrates with formation of ethanol increased by 0.79 % vol. as compared to a conventionally known strain during the same period of fermentation. The specificity of the wine material produced with usage of Saccharomyces cerevisiae Y-3973 strain in a finer aroma and taste.
 Strain of lactobacillus delbrueckii, reducing content of cholesterol in blood / 2539785
Group of inventions relates to biotechnology. Claimed is strain of Lactobacillus delbrueckii subspecies lactis CNCM I-3741, reducing content of cholesterol in blood. Strain is applied for obtaining fermented dairy products.
Arthrospira platensis (nordst) geitl rsemsu t/05-117 strain - lipid-containing biomass producer / 2539766
Invention relates to biotechnology. The Arthrospira platensis (Nordst.) Geitl. rsemsu T/05-117 strain has high content of neutral lipids. The strain is stored in the collection of the research laboratory of renewable energy sources of the Faculty of Geography of Moscow Lomonosov State University.
 Bacillus subtilis strain capable of splitting sugars and having antagonistic effect on pathogenic and opportunistic pathogenic bacteria and fungi, and use thereof / 2539762
Group of inventions relates to biotechnology. Disclosed is a Bacillus subtilis VKPM V-11353 strain, capable of splitting a wide range of mono- and di-sugars and a wide range antagonistic effect on pathogenic and opportunistic pathogenic bacteria and fungi, which cause diseases in plants and farm animals. Also disclosed are versions of using the Bacillus subtilis VKPM V-11353 strain as a bacterial preservative for silos, for producing agents for normalising intestinal microflora of animal farms and for producing agents for protecting plants from diseases.
Strain of bacteria paenibacillus sp. for obtaining biological product against diseases of wheat caused by phytopathogenic fungi / 2539738
Strain of bacteria Paenibacillus sp. IB-1 has antagonistic activity against phytopathogenic fungi. The strain is deposited in the Russian National Collection of Microorganisms under the registration number VKM B-2823D and can be used to produce the biological product for protection of plants against diseases caused by phytopathogenic fungi.
Method of cleaning soils from oil under conditions of low positive temperatures with psychrotolerant bacteria pseudomonas sp. ib-1.1 / 2539148
Method comprises application into soil of suspension of microbial preparation on the basis of suspension of psychrotolerant bacterial strain Pseudomonas sp. IB - 1.1 with a titre of not less than 2.0·108 CFU/ml.
Strain of bacteria desulfovibrio sp. for wastewater treatment from heavy metal ions / 2542402
Strain of bacteria Desulfovibrio sp. A 4/1 is deposited in the Russian Collection of Microorganisms (RCM) under the registration number of RCM B-2820D, and is resistant to heavy metal ions.
Nutrient medium for determining antibiotic susceptibility of legionella pneumophila / 2542390
Nutrient medium comprises enzyme-autolyzate of cattle spleen, monosubstituted potassium phosphate, disubstituted potassium phosphate, potassium gluconate, mesoinosite, soluble starch, gelatin, L-cysteine, iron (III) sodium salt, high-grade ink and distilled water in a predetermined ratio of components.
 Method for production of bacterial concentrate and its application as probiotic biologically active food additive / 2541778
Inventions group relates to biotechnology and may be used for preparing bacterial preparations applicable as probiotic biologically active additives. The bacterial concentrate production method envisages preparation of a nutritional medium, sterilisation and cooling. Inoculum introduction, cell propagation, bacterial mass separation from culture liquid, dispensing and closuring. Into the nutritional medium composition one introduces cedar or linseed oil of fish or ringed seal fat in an amount of 1-1.5% of the medium weight; the inoculum is represented by a Bifidobacterium longum DK-100 strain. The produced bacterial concentrate is used as a probiotic biologically active food additive.
Method for production of dry complex starter for kvass fermentation / 2541758
Method involves preparation of a saccharified brew by way of first grade wheat flour and wheat bran mixing at a ratio of 1:1, the produced mixture brewing with 85-90°C water, maintenance during 45-60 minutes, the mixture cooling to 65-67°C, saccharifying with non-fermented barley or rye malt in an amount of 10% of the mixture weight during 60-90 minutes, yeast autolysate introduction in an amount of 0.1% of the mixture weight to produce a nutritional substrate. Then one performs the substrate weighing out into three fermentative vessels with quadruplication of weighed portion equal to 50 g:200 g:800 g, respectively, three-times' autoclaving with a 24 hours' interval under 1.5 atm during 60 minutes, lactic acid bacteria reproduction in the prepared substrate in three-phase propagation cycle till the final acidity of sodium hydroxide solution with concentration equal to 1 mole/dm3 is equal to16-18 cm3 per 100 cm3 of the brew; the lactic acid bacteria source is represented by prebiotic preparations "Lactobacterin" or "Bifidumbacterin" "Evitalia" or a complex consisting of the said three preparations that are dissolved in 5 cm3 of a sterile physiological solution with each of them transferred into the cooled sterile saccharified brew in an amount of 1 dose of the preparation which is equal to 2×109, 107 and 1.5-2.0×109 CFU, respectively, per 50 g of the substrate. The inoculated nutrient medium is incubated (first phase) during 24 hours at a temperature of 37°C; then one performs the second and the third phases of the propagation cycle by way of dilution of the ripe starter of the previous phase in a four-fold quantity of the sterile saccharified brew for the subsequent phase. Incubation of the subsequent phases of the propagation cycle is performed during 24 hours at a temperature of 37°C till acidity of sodium hydroxide solution with concentration equal to 1 mole/dm3 is 16-18 cm3 per 100 cm3 of the brew. Then one performs the phases mixing with potato starch in an amount equal to the starter weight and Saccharomyces cerevisiae pressed bakery yeast in a dose of 5 g/100 dm3 of the kvass wort, the produced mass drying in a sterile air flow at room temperature till moisture content is equal to 13-14%. The total time of the saccharified brew fermentation is equal to 72 hours.
 Nutrient culture medium for swine erysipelas strain erysipelothrix rhuisipathie / 2541454
Nutrient culture medium for swine erysipelas strain Erysipelothrix rhuisipathie refers to general biotechnology and veterinary microbiology. As a nitrogenous nutrition source, the nutrient medium contains a mixture of fish autolysate and alkaline mussel hydrolysate in the following proportions: alkaline mussel hydrolysate 20-50%; fermentation peptone 1%; potassium phosphate 0.3%; sodium phosphate 1.8%; fish autolysate - the rest.
 Method of obtaining bacterial concentrate of bifidobacteria in liquid form / 2540022
Invention relates to biotechnology and medical industry and can be used in production of bacterial concentrates, biologically active food additives, fermented food products. Method of obtaining bacterial concentrate of bifidobacteria in liquid form includes preparation of nutritional medium with addition of growth components based on clarified cottage cheese whey or on water base with addition of up to 1.5% of glucose, or on soybean whey with addition of lactose in amount 1%. Strain of bifidobacteria B. bifidum 83, activated with β-halactosidase, is introduced into prepared nutritional medium in amount 3-5%, biomass is grown, cooled, poured in containers.
Barley-and-milk starter preparation method / 2540015
Invention relates to a starter preparation method. The method envisages preparation of the substrate of a mixture of barley flour and dry milk, mixed with water at a ratio of 1:3-1:4 and a temperature of 75-80°C, the substrate cooling, introduction of a mixture of amylase and xylanase enzyme preparations, maintenance at a temperature of 48-50°C during 90-120 min, the barley-and-milk hydrolysate cooling to 32-35°C, introduction of Linex combined preparation (1 capsule per 100 g of the barley-and-milk hydrolysate) and pressed bakery yeast (0.1% of the barley-and-milk hydrolysate total weight), the barley-and-milk starter incubation during 18-20 h at a temperature of 32-35°C, until titratable acidity is 10-12 degrees.
 Method for preparing pertussis component of combined vaccines / 2540014
Invention refers to biotechnology and concerns a method for preparing a pertussis component of complex vaccines. The presented method involves the B. pertussis culture growth on dense Bordet-Gengou and/or CCA nutrient media, the grown colonies selection according to the morphological characteristics, the selected colonies passage, the bacterial mass growth, the culture washout, the extinction reduction of the prepared pertussis suspensions to 10 international optical units, the agglutination reaction to measure agglutinogens 1, 2, 3 and the selection of pertussis suspensions, wherein the agglutinogen content is determined by type-specific serum dilution 1:3200 and more. The detected B. pertussis culture expressing agglutinogens 1, 2, 3 actively after the lyophilisation is used to prepare the pertussis component of complex vaccines.
Strain of lactobacillus delbrueckii, reducing content of cholesterol in blood / 2539785
Group of inventions relates to biotechnology. Claimed is strain of Lactobacillus delbrueckii subspecies lactis CNCM I-3741, reducing content of cholesterol in blood. Strain is applied for obtaining fermented dairy products.
Bacillus subtilis strain capable of splitting sugars and having antagonistic effect on pathogenic and opportunistic pathogenic bacteria and fungi, and use thereof / 2539762
Group of inventions relates to biotechnology. Disclosed is a Bacillus subtilis VKPM V-11353 strain, capable of splitting a wide range of mono- and di-sugars and a wide range antagonistic effect on pathogenic and opportunistic pathogenic bacteria and fungi, which cause diseases in plants and farm animals. Also disclosed are versions of using the Bacillus subtilis VKPM V-11353 strain as a bacterial preservative for silos, for producing agents for normalising intestinal microflora of animal farms and for producing agents for protecting plants from diseases.
Use of enzyme of ribonuclease bacillus pumilus as inhibitor of phytopathogenic virus / 2542480
Use of the enzyme of ribonuclease Bp as an inhibitor of development of RNA-genomic phytoviruses is proposed. In processing tobacco plants with ribonuclease Bp enzyme solution with the concentration of 100 µg/ml of distilled water, 94% of the plants are resistant to X-virus of tobacco.
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FIELD: biotechnologies. SUBSTANCE: method provides for pre-seeding treatment of seeds, soil irrigation and treatment of vegetative parts of plants with cultural liquor of strain Lactobacillus plantarum 60-DEP deposited in the All-Russian State Collection of Strains of Microorganisms, which are used in veterinary science and animal breeding, with a titre of 106 CFU/ml at the consumption of 5-30 ml per 100 ml of water. EFFECT: invention allows simplifying the method. 8 tbl, 3 ex
The invention relates to agricultural Microbiology, in particular to the use of the bacterial strain Lactobacillus plantarum as a stimulator of growth and development of plants, such as plants for agricultural purposes. Known method of promoting growth and protecting fruit crops from diseases caused by fungal pathogens, including processing plants, biological product in liquid form, RU # 2477596 C2, A01C 1/00, A01N 63/00, A61P 1/14, C12N 1/20, 20.03.2013. There is a method of combating pests and diseases of cruciferous crops by seed treatment composition comprising an insecticide, fungicide, biologically active trace element composition, growth and development of plants and water, EN No. 2468582 C1, A01N 65/00, A01P 3/00, 10.12.2013. You know the widespread use of the bacterial strain Lactobacillus plantarum in the form of pharmaceutical compositions for the preparation of cosmetics, food industry, veterinary medicine, feed production, agricultural Microbiology. Known bacteria strains Lactobacillus plantarum used in compositions to obtain probiotic preparations for the prevention and treatment of various diseases, RU # 2491081 C2, A61K 35/74, A61P 11/00, A61P 1/14, 27.08.2013; RU №2491079 C1, A61K 35/74, A23K 31/718, A61P 1/00, 27.08.2013; RU №2486234 C2, C12N 1/20, A61K 35/74, A23C 9/123, 27.06.2013; RU №2482177 C1, C12N 1/20, A61K 35/74, 20.05.2013; RU №2465320 C2, C12N 1/20, A61K 35/74, A61P 15/00, C12R 1/225, 27.10.2012; RU №2463067 C2, A1K 36/45, A61K 36/18, A23L 1/29, A61P 3/10, 10.10.2012; RU №2440123 C2, A61K 35/74, A23K 39/02, C12R 1/25, A61P 31/12, 27.10.2012; RU №2418063 C2, C12N 1/20, A61K 35/74, A61P 31/16, A61P 11/00, 10.05.2011; RU №2417092 C2, A23K 35/74, A61P 37/00, 27.04.2011; RU №2402341 C2, A23K 35/74, A23C 9/123, A23L 1/03, A61P 19/10, C12R 1/20, 27.10.2010; RU №2376366 C2, C12N 1/20, A23K 35/74, A23C 9/12, A23L 1/30, 20.12.2009; RU №2356942 C2, C12N 1/20, A61K 31/35, A61K 35/74, A23L 1/30, 27.05.2009; RU №2326938 C2, C12N 1/20, A61K 35/74, A23C 9/123, 20.06.2008; RU №2316586 C2, C12N 1/20, A23L 15/26, A61K 35/74, A23C 9/123, 2008; RU №2224017 C2, C12N 1/20, A61K 35/74, A61L 15/36, 20.02.2004; RU №2061040 C1, C12N l/20, 27.05.1996. It is known the use of the bacterial strain Lactobacillus plantarum in the field of cosmetology, RU # 2459864 C2, C12N 1/20, A61K 8/99, 27.08.2012; RU №2351376 C1, A61Q 19/08, 10.04.2009. Known bacterial strain Lactobacillus plantarum as antimicrobial probiotic used to prepare dairy products for the production of starter cultures for various purposes, for the fermentation of raw meat and its products, for the production of bread, RU # 2477750 C2, C12N 1/20, A61K 35/74, A23C 9/123, C12R 1/25, 20.03.2013; RU №2409034 C1, A21D 8/02, A21D 8/04, 20.01.2011; RU №2409033 C1, A21D 8/02, A21D 8/04, 20.01.2011; RU №2391845 C2, A23C 15/00, A23C 15/02, 20.06.2010; RU №2373270 C2, C12G 3/02, 20.11.2009; RU №2367685 C1, C12N 1/20, 20.09.2009; RU №2345676 C1, A23L 2/02, A23L 1/29, 10.02.2009; RU №2345675 C1, A23L 2/02, A23L 1/29, 10.02.2009; RU №2339258 C2, A23L 2/04, 27.11.2008; RU №2264114 C1, A23C 9/12, A23C 9/13, 20.11.2005; RU №2139346 C1, C12N 1/20, A61K 35/74, 10.10.1999; RU №2102473 C1, C12N 1/20, A22C 11/00, 20.01.1998. Known probiotic preparations on the basis of the bacterial strains Lactobacillus plantarum related to the field of medicine, veterinary medicine, biotechnology, to obtain the means of prevention and lichenicolous and farm animals, RU # 2268926 C2, C12N 1/20, A23C 9/12, 27.01.2006; RU №2247148 C2, C12N 1/20, A61K 35/74, 27.02.2005. Know the use of the bacterial strain Lactobacillus plantarum for fodder production, RU # 2423518 C1, C12N 1/20, A23K 1/16, 10.07.2011; RU №2409661 C2, C12N 1/20, C12P 21/00, 20.01.2011; RU №2391859 C2, A23K 1/14, A23K 1/16, 20.06.2010; RU №2390554 C2, C12N 1/20, C12P 21/00, C12R 1/25, 27.05.2010; RU №2380107 C1, A23K 35/66, A23K 1/165, 27.01.2010; RU №2378000 C1, A61K 35/74, 10.01.2010; RU №2350101 C2, A23K 1/16, 27.03.2009; RU # 2341100 C2, A23K 1/00, A23K 1/16, A61K 35/74, 20.12.2008; RU №2268299 C2, C12N 1/20, A23K 3/02, 20.01.2006; RU №2265655 C2, C12N 1/20, A23K 3/02, 10.12.2005; RU №2250265 C2, C12P 7/06, 20.04.2005; RU №2244001 C1, C12N 1/20, C12P 21/00, 10.01.2005; RU №2168909 C1, A23K 3/02, C12N 1/20, 20.06.2001. Know the use of a consortium of bacteria Lactobacillus plantarum in agricultural Microbiology, RU # 2374211 C2, C05F 3/00, 27.11.2009; RU №2362295 C1, A01F 25/00, 27.07.2009; RU №2162833 C2, C05F 11/08, C05F 3/00, C12N 1/20, C12P 39/00, 10.02.2001. In the known methods using bacteria strains Lactobacillus plantarum 60, stimulating the growth and development of plants, not found. Known biological preparation to enhance the productivity of agricultural crops, including bacterial strain Lactobacillus plantarum that is used in the form of cultural liquid for presowing treatment of seeds, soil and processing of vegetative parts of plants, RU # 2264999 C2, C05F 11/08, 27.11.2005. This solution is accepted as the closest analogue of the present invention. Biologic closest analogue relates to the field of agricultural Microbiology and including the et a mixture of live microbial cultures of Rhodopseudomonas palustris 100, Rhodobacter spheroides 5, Lactobacillus casei 21, Lactobacillus plantarum 51, Lactococcus lactis 47 and Saccharomyces cerevisiae 22 in a predetermined ratio of the components. Biological product increases the productivity of agricultural crops, stimulates the development of natural microbial soil and plants. However, it is designed on the basis of groups of microorganisms, which complicates the biotechnological process of obtaining a biological product. The present invention is based on a solution that allows to simplify the biotechnological process and to expand the technological capabilities when using the bacterial strain Lactobacillus plantarum 60-DEPT. The technical result of the present invention is a positive influence on the growth and development of plants due to the processing plant culture fluid on the basis of a single bacterial strain Lactobacillus plantarum 60 - DEPT optimal flow of the culture fluid at pre-sowing seed treatment, shedding of the soil and the processing of vegetative parts of plants. According to the invention this problem is solved due to the fact that the way to promote crop growth is a pre-sowing treatment of seeds, in the Strait of soil and in the processing of vegetative parts of plants of the culture fluid in the presence of the bacterial strain Lactobacillus plantarum. As the strain of bacteria used Lactobacillus plantarum 60-DEPT and predposevnoi the processing of seeds, Strait soil and processing of vegetative parts of plants hold the culture liquid with a titer of 106CFU/ml at a flow rate of 5-30 ml 100 ml Voy. The applicant has not identified the sources containing information about technical solutions, identical to the present invention, which allows to make a conclusion about its compliance with the criterion of "novelty". Due to the implementation of the distinguishing features of the invention (in conjunction with the features indicated in the restrictive part of the formula) achieved an important new properties object. The use of the culture fluid with a titer of 106CFU/ml based on the bacterial strain Lactobacillus plantarum 60 simplifies biotechnological process. The use of the culture fluid at a flow rate of 5-30 ml per 100 ml of water expands the range of possibilities of technological operations when conducting pre-sowing treatment of seeds, the Strait of soil and treatment of vegetative parts of plants. The applicant is not aware of any publication that would contain information about the impact of the distinctive features of invention technical result achieved. In this regard, according to the applicant, it is possible to draw a conclusion on the conformity of the proposed technical solution the criterion of "inventive step". Lactobacillus plantarum 60-DEPT - representative of the family Lactobacillaceae of the genus Lactobacillus are used as production is i.i.d. probiotic preparation in ensiling of forages. The strain deposited 29.06.2001 year. Registration number - strain Lactobacillus plantarum 60-DEPT in the all-Russian state collection of strains of microorganisms used in veterinary medicine and animal husbandry (123022, , Moscow, highway, and 5). Left strain - OOO Biotrop". Lactobacillus plantarum 60 isolated as a spontaneous mutant strain of Lactobacillus plantarum 52. The culture is stored on environment Wort broth with chalk at a temperature of 4-6°C. Gram "+" still sticks, arranged singly or in pairs. Optional gone anaerobic. When cultivation on Mrs-broth formed white precipitate, which dissolved with shaking, on Mrs-agar microorganism produces a creamy-yellow round colonies, easily removable from the agar. On Wort-agar forms a round white colonies with smooth edges. Ferments lactose, maltose, rhamnose, sorbitol, dulcet, sucrose, galactose, slightly fermented to arabinose and raffinose without the formation of gas. Restores the milk with the addition of methylene blue and milk with the addition of litmus paper. Does not grow on milk with added 2%, 4%, 6% NaCl, 0.4% phenol. Milk with added bile growth is good. Catalase - negative microorganism. Optimum pH is 6.5-7.0. Growth at a temperature of 37°C. Pathogenicity to animals - negative. Species identification of strains of the microorganism is in Samples of the culture fluid were analyzed by molecular genetic methods sequencing. DNA from the culture fluid was isolated according to the standard Protocol Set for isolation of genomic DNA from various sources". Set a key part of the DNA sequence of the bacterial strain Lactobacillus plantarum 60-DEPT. Sequence:
Lactobacillus plantarum strain 60 16S ribosomal RNA gene, partial Sequence (100%). Link: http://www.ncbi.nlm.nih.gov/nuccore/KF387575 Nutrient medium for growing the bacterial strain Lactobacillus plantarum 60 - DEPT In a small volume of distilled water melt the agar in the amount of 2.5 g per 1 DM3cooking environment. To the rest of the distilled water, add 10 g of peptone, 40 cm3processed corn extract, 6.6 g of sodium citrate translesanas, 0.12 g of magnesium sulfate, 2 g of potassium phosphate disubstituted. The mixture is heated to a temperature of (80±2)°C, after which connect with the molten agar, add 10 g of glucose and 0.15 g of cysteine hydrochloric acid or 0.5 g of ascorbic acid. The entire mixture is topped up with hot distilled water up to a specified amount (1 DM3) and set (7,05±0,05) pH units using a 40% aqueous solution of sodium hydroxide. Wednesday poured into test tubes (12±2) ml and closed with cotton-gauze, Seeley is Anonyme or rubber plugs. Sterilization is carried out in an autoclave at a temperature of (112±5)°C (pressure 0.1 MPa) for (30±1) minutes. To test for sterility test tubes with the medium is incubated in a thermostat at a temperature of (37±1)°C for 2-3 days. Upon expiration of the specified term note visually the presence or absence of microbial growth on the surface of the agar. For planting use tubes in which no growth of microorganisms. Compounding semi-corn-glucose environment: - Peptone - 10 g/l; - Processed corn extract - 40 cm3; - Sodium citrate trehzameshchenny - 6.6 g/l; - Magnesium sulfate - 0.12 g/l; - Potassium phosphate disubstituted - 2 g/l; Agar - 2.5 g/L. The culture fluid from the strain of the bacteria Lactobacillus plantarum 60-DEPT meets the following characteristics: Appearance and color - liquid light brown (presence of sediment); The concentration of microbial cells of Lactobacillus plantarum 60-DEPT 1 ml of culture liquid of not less than 1×106CFU/cm3; - Titratable acidity of not less than 30°T; - The presence of pathogenic organisms (bacteria p. Salmonella; enteropathogenic E. coli; toxinproducing anaerobes; any aerobic and facultative anaerobic microflora) is not allowed; - Non-toxic. Getting uterine culture The culture of the collective is ion tubes subcultured prick in semi-corn-glucose medium and cultivated at (28±1)°C until the rich growth zone around the injection in test tubes (2nd passage). Obtain seed culture. Culture of cells from the test tubes pipette bring in bottles with a liquid nutrient medium and carry out the cultivation at (28±1)°C for 2-3 days (passage 3). Stored uterine culture in test tubes at a temperature (2-4°C) for 1 month. Quality control uterine culture on the physiological condition and the absence of extraneous microflora are conducted once a month. All work associated with the preparation of inoculum (inoculation on culture media, breeding), produced in a special box, equipped with supply and exhaust ventilation with filter elements of thin air purification FG 315, WFR 90×50; 70×40; 60×35. Sowing culture in test tubes produced in microbiological Boxing microbial loop in the sterile area, using the sterilizer loops SteriMax WLD-TEC, or sterile disposable polymer loop. As the source of culture using the culture of the industrial Museum of Lactobacillus plantarum 60-DEPT. Each tube is seeded with a "prick". Cropped shoals tubes or vials are grown in an incubator at a temperature (28±1)°C for (36±12) hours. The temperature is controlled by the contact thermometer and record in the process log. For control of seed taken 2-5% test tubes and test them for purity and morphology of ognore the ability of culture. After 24 hours, carry out a preliminary examination of typical growth. The growth of culture should be abundant and uniform along the entire length of the injection, the colony of white color on the surface is not allowed growth of foreign microflora in the form of a film or a separate colonies of unknown origin. In a "squashed drop" should be detected typical homogeneous coli Lactobacillus plantarum 60-DEPT. Uterine culture tubes stored in a refrigerator at a temperature of (3±1)°C for 2 months. Quality control uterine culture on the physiological condition and the absence of extraneous microflora spend once a month, the results are recorded in the journal movement strain. Experimental studies on the use of the culture fluid with a titer of 106CFU/ml based on the bacterial strain Lactobacillus plantarum 60-DEPT confirmed by Examples 1-3. Example 1 Evaluation of the effect of bacterial strain Lactobacillus plantarum 60-DEPT on speed, vigor and laboratory germination of seeds. Selected 100 seeds of Timothy grass and 30 seeds of clover, wheat, barley and soak for 2 hours in a solution culture fluid on the basis of the bacterial strain Lactobacillus plantarum 60-DEPT in various concentrations. The seeds are soaked in an amount of 5, 10, and 30 ml of the culture fluid with a titer of 106CFU/ml per 100 ml of water. In the control seeds C is Machaut in the water. The treated seed laid on moistened filter paper in sterile Petri dishes (5 ml of water per Cup). Accounting laboratory germination of seeds is performed on the 1st and 3rd day. Estimates of the effects of pre-sowing treatment of seeds of agricultural plants at the initial growth processes are presented in table 1. As the table shows, a significant stimulating effect of strain was observed only in the seeds of barley, largely infected by phytopathogenic fungi p. Fusarium: indicators of the mass of sprouts, length of stem and root increased 2-2 .5 times compared with the control. On an uninfected seeds of barley and spring wheat stimulating effect was not observed. A slight increase in the length of the stem (6-12%) was observed in seedling clover. Root length was significantly increased in seedlings Timothy when using a dilution of 1:10 (table.1) Effect of presowing treatment of the culture fluid of the bacterial strain Lactobacillus plantarum 60 - DEPT on field germination of seeds of various crops is presented in table 2. Analysis of experimental results shows that the germination was significantly increased after treatment of the culture fluid of the bacterial strain Lactobacillus plantarum 60 - DEPT, and this effect was observed in all crops. But Uwe is ikenie germination did not exceed 3-7%. More significantly, up to 20%, germination was increased only in the seeds of barley. Example 2 Evaluation of the effect of bacterial strain Lactobacillus plantarum 60-DEPT on growth rate of plants. To determine the effect of bacterial strain Lactobacillus plantarum 60-DEPOT at the initial growth processes selectively from each of the Petri dishes selected 10 seedlings seeds of each crop, weigh, measure the length of the root and stem length of the sprout. Within 10 days after sowing spend Strait soil culture fluid of bacterial strain Lactobacillus plantarum 60-DEPT (5 ml of the culture fluid with a titer of 106CFU/ml per 100 ml of water). The studies revealed a significant effect of bacterial strain Lactobacillus plantarum 60-DEPT on the intensity of plant growth (table.3-5). Effect of presowing seed treatment and spraying the culture fluid of the bacterial strain Lactobacillus plantarum 60-DEPT on plant height, Timothy meadow presented in table 3. From table 3 it can be seen that the treatment of the culture fluid of the bacterial strain Lactobacillus plantarum 60-DEPT had a positive effect on plant growth, Timothy and amounted at the end of the growing season of 12.8%. Effect of presowing seed treatment and spraying the culture fluid of the bacterial strain Lactobacillus plantarum 60-DEPT on the height of plants of barley (clean seeds) are presented in table 4. Table 4 shows that the creature is Noah stimulating influence of the culture fluid of the bacterial strain Lactobacillus plantarum 60-DEPT on growth rate of plants identified on the culture of barley in the variant of the experiment with seeds, not infected seed infection. The stimulating effect was observed throughout the growing season and reaches its end of 11-13%. Effect of presowing seed treatment and spraying the culture fluid of the bacterial strain Lactobacillus plantarum 60-DEPT on the height of plants of red clover are presented in table 5. From table 5 it is seen that the application of integrated seed treatment, soil application and spraying of plants culture fluid of bacterial strain Lactobacillus plantarum 60-DEPT significantly influenced the plant height. Example 3 The influence of the culture fluid of the bacterial strain Lactobacillus plantarum 60-DEPT of basic biometric indicators of growth and development of crops Pre-sowing treatment is carried out by soaking the seeds, Timothy, clover, barley in working solution culture fluid of bacterial strain Lactobacillus plantarum 60-DEPT (5 ml of the culture fluid with a titer of 106CFU/ml per 100 ml of water) for 2 hours followed by drying in air. Then the seeds are sown in ceramic vessels with prepared soil. For the experience of taking the seeds of clover and barley in the amount of 30 pieces, Timothy - 100 pieces After 10 days spend Strait soil working solution culture fluid of bacterial strain Lactobacillus plantarum 60-DEPT (5 ml of the culture fluid with a titer of 106CFU/ml per 100 m of the water). After 15 days after sowing the seeds carry out spraying of seedlings of plants in all variants of experience working solution culture fluid of bacterial strain Lactobacillus plantarum 60-DEPT (5 ml of the culture fluid with a titer of 106CFU/ml per 100 ml of water). Accounting biometric growth and development of plants carried out by standard methods for Gorbatschow. Determine the height of the plants, moist and air-dry biomass, separate raw and air-dry mass of the root system and above-ground parts of plants. All experiments are performed in a 4-fold repetition, the results are processed statistically methods of variance analysis. The studies revealed a significant effect of bacterial strain Lactobacillus plantarum 60 on biometrics growth and development of plants (PL.6-8). Effect of presowing seed treatment and spraying the culture fluid of the bacterial strain Lactobacillus plantarum 60-DEPT on biometrics growth and development of plants, Timothy meadow presented in table 6. From table 6 it is seen that the total mass of plants, as weight per plant increased in 2-3 times, the total mass of the aerial part and the mass of the aerial part of a plant - 2.9-4.4 times compared with the control. The total mass of roots and average weight of root per plant was increased not so much, but exceeded the screening of the teli control 2-2,75 times. Effect of presowing seed treatment and spraying the culture fluid of the bacterial strain Lactobacillus plantarum 60-DEPT on biometrics growth and development of plants of red clover are presented in table 7. Table 7 shows that the total mass of plants, as the weight of one of the plants has increased by almost 30%, the total mass of the aerial part and the mass of the aerial part of a plant - 27-40% compared with control. The total mass of roots and average weight of root per plant was higher than control at 15-23%. Effect of presowing seed treatment and spraying the culture fluid of the bacterial strain Lactobacillus plantarum 60-DEPT on biometrics growth and development of barley are presented in table 8. From table 8 it is seen that the total mass of plants, as weight per plant, increased by 15-20%, the total wet mass of the aerial part and the mass of the aerial part of a plant - 21-25% compared with control. The total dry mass of the aerial part and the mass of the aerial part of a plant has exceeded that of the control 12-18%. Thus, studies have shown that the culture fluid of the bacterial strain Lactobacillus plantarum 60-DEPT has a significant positive impact on the growth and development of agricultural colour. Pre-treatment culture fluid of bacterial strain is Lactobacillus plantarum 60-DEPT increases the germination of seeds of agricultural crops on 3-8% and 20% increases the germination of seeds of barley. The use of cultural liquid of the bacterial strain Lactobacillus plantarum 60-DEPT has a stimulating effect on the growth rate of plants, especially grasses. Plant height, Timothy field increased by 13-35%, and clover - 20-22% compared to control. On culture, Timothy meadow samples treated with the culture fluid of the bacterial strain Lactobacillus plantarum 60-DEPT, increased the total mass of the plants 2-3 times the mass of the aerial - 2.9-4.4 times compared with the control. The weight of the root system has exceeded that of the control 2-2,75 times. On the clover significant positive impact on the growth and development of plants treated with the culture fluid of the bacterial strain Lactobacillus plantarum 60-DEPT total weight of the plants increased by almost 30%, the mass of the aerial part - 27-40%, the root system on 15-23% compared to control. In barley revealed a significant positive influence on the accumulation of total plant mass and the mass of their aboveground parts - by 21-25% compared to control. The proposed method of stimulating the growth of agricultural crops carried out using well-known biotechnology, which is widely used in agriculture, and carried out laboratory works LLC BIOTROP" condition, according to the applicant, his criterion of "industrial is eminimall". The proposed method allows us to simplify biotechnological process due to the use for the preparation of the culture fluid from one bacterial strain Lactobacillus plantarum 60-DEPT (in contrast to the closest equivalent) and to expand the technological capabilities when using the bacterial strain Lactobacillus plantarum 60-DEPT expense of choice when conducting pre-sowing treatment of seeds, the Strait of soil and treatment of vegetative parts of plants the culture fluid at a flow rate of 5-30 ml per 100 ml of water depending on the type of culture and technological processing.
Way to stimulate crop growth, which consists in pre-sowing treatment of seeds, in the Strait of soil and in the processing of vegetative parts of plants of the culture fluid in the presence of the bacterial strain Lactobacillus plantarum, characterized in that as the strain of bacteria used Lactobacillus plantarum 60-DEPT deposited in the all-Russian state collection of strains of microorganisms used in veterinary medicine and animal husbandry, and pre-sowing seed treatment, shedding of the soil and the processing of vegetative parts RA the plants hold the culture liquid with a titer of 10 6CFU/ml at a flow rate of 5-30 ml per 100 ml of water.
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