Method for making plate from modified xenogeneic enteral submucosa
SUBSTANCE: method for making a plate from modified xenogeneic enteric submucosa involves mechanical evacuation of small bowel, processing in hypertonic saline in a combination with ultrasonic exposure, enzymatic treatment, subsequent washing in acetic acid and sodium hydrocarbonate, sodium chloride processing, additional ultrasonic exposure and enzymatic treatment, storage in acetic acid and sodium hydrocarbonate, storage in an antimicrobial agent and multiply changed ascending glutaric dialdehyde. The biotissue is stored in multiply changed ascending glutaric dialdehyde in the melted form between the two plates of porous non-woven material.
EFFECT: preparing the non-immunogenic biomaterial uniformly cross linked throughout with particularly damaged collagen structure that provides the fast recipient's cell penetration into the transplant and smooth invasion of vessels and tissues in the postoperative period.
The present invention relates to medicine, in particular for prosthetics affected areas of organs and tissues, dentistry, combustology, as well as to the field of tissue engineering, in particular to the manufacture of substrates, scaffolds to check their donor cells.
There are many ways of processing submucosal membrane of the small intestine with the purpose of its further application in medicine.
So, one of the patents describes a tissue graft, comprising a shell submucosal basis of a segment of small intestine of warm-blooded vertebrate. Shell submucosal basics layer is separated from the muscle sheath and at least from the abdominal part of the mucous membrane (U.S. Pat. RF 2037317 C1 IPC6, A61B 17/00). Further, the biomaterial is placed in a solution of neomycin sulfate. The disadvantage of this method of preparing a tissue graft is that the fabric is not subjected to decellularization and chemical processing, which would reduce the degree of immunogenicity of the biomaterial, as well as to monitor its biodegradation. Also the material is processed with a stabilizing agent, then its strength is increased.
Closest to the claimed method of manufacturing submucosal membrane of the small intestine is the method described in the patent of the Russian Federation 2438713 navigated machining thin the intestine, her sterilization, degreasing, the fixation cross stitching, minimizing antigenic activity and adherence of the active layer. However, this method of processing biological tissue does not provide for the complete removal of cellular elements of the collagen matrix, which may lead to a possible immune response in the patient's body.
The proposed method provides mechanical cleaning of the small intestine from the mucous and muscular layers, pre-treatment in hypertonic salt solutions of sodium chloride with simultaneous exposure to ultrasound, the enzymatic treatment of submucosal membrane of the small intestine, washing in a solution of acetic acid and sodium bicarbonate, treatment with sodium chloride, the additional impact of ultrasound and re enzymatic processing with subsequent curing of the biomaterial solution of acetic acid and sodium hydrogen carbonate solution, exposure to antimicrobial solution, and then cross-linking agent.
The purpose of this method is to reduce the risk of immune response to the transplant, partial destruction of collagen structures submucosal membrane of the small intestine to provide a more rapid and unimpeded sprouting of blood vessels and body tissues of the patient at the plate on the basis of the modified xenogeneic submucosal membrane thin the intestine.
The essence of the method lies in the fact that biological tissue prior to enzymatic treatment is cleaned mechanically and placed in hypertonic solutions of sodium chloride and exposed to ultrasound at least once during 20-300 minutes, then treated with a proteolytic enzyme at a concentration of 0.1-10 PE 1 gram of wet tissue, washed in a solution of acetic acid and sodium bicarbonate, and incubated in hypertonic solutions of sodium chloride, then further exposed to ultrasound and enzymatic processing in acetate buffer solution with a concentration of the enzyme is not less than 10.1 PU per gram of wet tissue with subsequent curing of the biomaterial solution of acetic acid and sodium hydrogen carbonate solution, whereupon the submucosal membrane of the small intestine is treated with an antimicrobial agent and repeatedly smenauscheesa solutions of glutaraldehyde increasing concentrations straightened as between the two plates of a porous non-woven material (for example, a porous film material made from a mixture of cellulose acetates) and sterilized.
Mechanical cleaning of the small intestine, muscular and mucous layer is carried out by exposure to the gut set shaft, compresses and extrudes the above layers. It is also possible application is the development of other methods of mechanical treatment of the small intestine to get out of it submucosal shell: rubbing, the scraping and so on.
In the pre-treatment in hypertonic solutions of sodium chloride purified submucosa membrane of the small intestine is subjected to ultrasonic treatment at least once in the course of 20-300 minutes, and the enzymatic treatment is carried out in acetate buffer solution, using 0.1-10 PE of proteolytic enzyme per gram of wet tissue.
The processing time of the biomaterial with ultrasound, smaller than 20 minutes is not enough for the effective destruction of cellular elements, and more than 300 minutes is not reasonable, because the process of destruction of cellular elements will be completed.
The enzymatic processing guarantees the destruction left after ultrasonic treatment of cellular elements submucosal membrane of the small intestine and proteins as the main carriers of antigenicity.
The concentration of the proteolytic enzyme is less than 0.1 PE may not be effective for the destruction of the remaining cells, tissues and proteins. The concentration of more than 10 PE is not appropriate, because the concentration of enzyme in 10 NE enough to process the complete destruction of the cell.
Order inactivation effects of proteolytic enzymes on submucosal membrane of the small intestine change the parameters on which depends the activity f is rment, namely, the temperature of the acetate buffer solution, pH. For this biomaterial is kept in a cooled acidic solution and repeatedly washed in running distilled water.
To neutralize remaining after washing with acetic acid biomaterial submucosal membrane of the small intestine is placed in a solution of sodium bicarbonate, then incubated in hypertonic salt solutions to extract the products of proteolysis of cells and proteins from tissues and washed with distilled water.
Repeated exposure of ultrasound on submucosal membrane of the small intestine and re-processing enzyme solution at a concentration of more 10,1 PE per gram wet tissue with subsequent curing of the biomaterial solution of acetic acid and sodium hydrogen carbonate solution leads to the partial destruction of collagen structures submucosal membrane of the small intestine without significant changes in its physical and mechanical properties that will provide a more rapid penetration of the tissue cells of the recipient to the graft and smooth the sprouting of blood vessels and body tissues of the patient in the postoperative period. The concentration of the proteolytic enzyme is less than 10.1 PE per gram wet tissue is not sufficient to start the destruction of collagen fibers.
Keeping podkisst the second membrane of the small intestine in antimicrobial agent will eliminate microbial activity on the surface of the submucosal membrane of the small intestine. As the antimicrobial agent can be an antibiotic of broad or narrow spectrum of activity, or the agents based on silver.
Treatment of submucosal membrane of the small intestine solutions of glutaraldehyde increasing concentrations necessary to stabilize the fabric, reducing its immunogenicity. Keeping submucosal membrane of the small intestine in solutions of glutaraldehyde is straightened as between the two plates of a porous non-woven material. This allows to get a smooth plate, without folds, uniformly impregnated with a crosslinking agent. The use of porous material provides a sufficient degree of contact with the submucosal membrane of the small intestine with a solution glutarovogo aldehyde.
A method of manufacturing a plate on the basis of the modified xenogeneic submucosal membrane of the small intestine submucosal membrane of the small intestine is performed in the following way.
The small intestine thoroughly washed several times with internal and external parties running water and cut in the longitudinal direction. Then produce a division of the small intestine into sections with a length of 10-40 cm by cutting.
Next, perform a mechanical cleaning of the small intestine, muscular and mucosal layers by extrusion between the two shafts. You can also use other methods IU onicescu cleaning of the small intestine to get out of it submucosal shell: rubbing, the scraping and so on.
Received submucosal membrane of the small intestine is placed in a container with a hypertonic solution of sodium chloride at a concentration of 1-10% and leave on 24-76 hours, changing every day, hypertonic solution of sodium chloride in the container. During this processing submucosal membrane is exposed to ultrasound for 50 minutes. After processing, the material is washed in distilled water. Next, perform a processing of a proteolytic enzyme. To do this, calculate the amount of proteolytic enzyme, necessary for the process: 0,1-10 PE 1 gram of wet tissue and dissolve it in acetate buffer solution, heated to 37°C. After treatment with the enzyme submucosal membrane is kept in inactivating solution (6-60 g of acetic acid, 100 g of sodium chloride, 840-894 g of distilled water) not less than 20 minutes, washed in distilled water and placed in a 0.1 M solution of sodium bicarbonate, then washed with distilled water. Then hold postfermentation processing hypertonic solutions of sodium chloride 2% and 7% concentration within 24-76 hours, then exposed to ultrasound for 40 minutes, washed in running distilled water and re-produce enzymatic processing solution with a concentration of proteolytic the ski enzyme 10,1 PE over one gram of tissue with subsequent curing of the biomaterial solution of acetic acid and sodium hydrogen carbonate solution, washed in running distilled water. Then make processing of submucosal membrane of the small intestine by triclosan and solutions of glutaraldehyde increasing concentrations straightened as between two porous nonwoven plates.
The small intestine clear of muscular and mucosal layers by scraping them into submucosal membrane of the small intestine, which is placed in a container with a hypertonic solution of sodium chloride at a concentration of 3% and leave for 68 h, producing daily replacement of a hypertonic solution of sodium chloride, then hold shift for a 7% solution of sodium chloride and leave submucosal membrane of the small intestine for 5 hours in this solution. During the processing of biological tissue once exposed to ultrasound for 110 minutes. After a time pre-treatment samples repeatedly washed in distilled water and carry out enzymatic processing. To do this, calculate the amount of proteolytic enzyme needed to process: 1 PE 1 gram of wet tissue and dissolve it in acetate buffer solution, heated to 37°C. After enzyme treatment, the biomaterial is kept in inactivating solution of acetic acid (6-60 g of acetic acid, 100 g of sodium chloride, 840-894 g of distilled water) for at least 20min, washed repeatedly in distilled water and placed in 0.1 M sodium hydrogen carbonate solution and again washed with distilled water. The material is then placed in a hypertonic salt solutions at concentrations of 3% and 7% for 15 and 6 h, respectively, and washed in running distilled water. Then hold the additional impact of ultrasound on submucosal membrane of the small intestine within 60 minutes and re-enzymatic treatment with a concentration of proteolytic enzyme 12,5 PE per gram wet tissue for 5 minutes, followed by curing of the biomaterial solution of acetic acid and sodium hydrogen carbonate solution, as described above. Then submucosal membrane of the small intestine incubated in 0.3% solution of triclosan within 24 hours and placed in a solution of glutaraldehyde concentration was low in the unfolded between the two plates of a porous non-woven material for 72 h, then make shift solution of glutaraldehyde low concentration in a solution of glutaraldehyde higher concentration.
Differs from example 1 in that submucosal membrane of the small intestine is placed in a container with a hypertonic solution of sodium chloride at a concentration of 3% at 48 h, and then at 7% hypertonic sodium chloride solution for 12 h, is subjected to the action of ultra is sound for 50 minutes, processing proteolytic enzyme is carried out at 5 PE 1 gram of wet tissue and a second enzymatic treatment is carried out at 10,2 PE per gram wet tissue for 10 minutes.
Differs from example 1 in that submucosal membrane of the small intestine is treated with an antimicrobial agent, which is gentamicin.
Differs from example 1 in that the small intestine clear of muscular and mucosal layers by punching through the shafts.
Application of the proposed method of manufacture plate on the basis of the modified xenogeneic submucosal membrane of the small intestine will get a non-immunogenic, evenly stitched throughout the volume of the biomaterial with partially destroyed collagen structure that will provide a more rapid penetration of recipient cells in the graft and smooth the sprouting of blood vessels and body tissues of the patient in the postoperative period.
Sources of information
- RF patent №2037317,
- RF patent №2438713.
A method of manufacturing a plate on the basis of the modified xenogeneic submucosal membrane of the small intestine, intended for use in tissue engineering and artificial organs and tissues, including mechanical cleaning of the small intestine, the treatment in hypertonic solutions of chloride of soda is I with the simultaneous influence of ultrasound, enzymatic processing, sequential washing in a solution of acetic acid and sodium bicarbonate, treatment with sodium chloride, additional processing ultrasound high frequency and re-enzymatic processing carried out in acetate buffer solution with a concentration of the enzyme is not less than 10.1 PE over one gram of tissue, the shutter speed in the solution of acetic acid and sodium hydrogen carbonate solution, the exposure to the antimicrobial agent and repeatedly samenaide solutions of glutaraldehyde increasing concentrations, with the extract of tissues in repeatedly samenaide solution of glutaric aldehyde is carried out in expanded form between the two plates of a porous non-woven material.
SUBSTANCE: group of inventions refers to a bone implant matrix, which contains the matrix base treated or needed to be treated with a reinforcing mixture consisting of a polymer and an additional ingredient. The bone implant matrix is in particular applicable for plastic orthopaedic surgery, orthopaedics, and specifically in maxillofacial surgery, maxillofacial and dental implantology. What is described is a method for preparing the bone implant matrix.
EFFECT: matrix base and polymer, which are the ingredients of the reinforcing mixture, promote the growth of a new bone integrated into surrounding tissues.
23 cl, 5 dwg, 3 ex
SUBSTANCE: group of inventions deals with medical prostheses for implantation into a human organism and methods of their manufacturing, in particular prosthesis of the jaw bone, which can be used in cosmetic surgery of the jaw bone or in the jaw bone reconstruction. Claimed is an implant of the jaw bone, manufactured in accordance with the method, including the following stages: selection of material of animal origin from an organism of cattle or pigs, with the material of animal origin being the jaw bone; shaping the material of animal origin to obtain a desirable shape of the jaw bone implant; removal of cells from the material of animal origin, crosslinking of the material of animal origin; removal of antigens from the material of animal origin; subjection of the material of animal origin to alkaline processing; introduction of active substances, improving adhesion on the implant of the growth factor and stem cells, produced by the human organism into the material of animal origin; packing the material of animal origin into a container with a sterilising solution. The jaw bone implant, manufactured by the said method, possesses high tissue compatibility.
EFFECT: group of inventions provides elimination of development or, at last, minimisation of phenomena of various types of discomfort under an impact of moving muscles in the period of the implant growing into tissues of the host organism, resorption and replacement with the new bone tissue with simultaneous provision of high tissue compatibility with elimination, or, at least, minimisation of phenomena of immunological rejection of a biological prosthesis of the jaw bone.
14 cl, 1 ex, 4 dwg
SUBSTANCE: method involves skin sampling from a dead donor in an operating room with using a skin grafting knife according to the standard procedure in compliance with aseptic and antiseptic regulations. Immediately after preparation, the biological material is placed in a sterile container with an aqueous solution of broad-spectrum antibiotic. The container is hermetically sealed keeping them sterile. The biomaterial is stored at -40°C until the donor's pathoanatomical findings have been obtained, and biological safety of the donor's tissues has been studied. The biomaterial with the proved biological safety is used for preparing the DM. Preparing the DM involves the following stages: separating the epidermis and the derma, conducting decellularisation of the derma, providing biocompatibility of the graft.
EFFECT: method enables reducing the production process to 36 hours and provides preparing the cell-free, biocompatible dermal matrix up to 1 mm thick with preserving fibre structures and direction.
2 cl, 2 tbl, 1 dwg
SUBSTANCE: invention refers to medicine. What is described is a method for preparing a cell-free organic tissue of a human or animal origin for the vitality recovery, particularly for introducing living cells, involving a stage of making a number of holes (4; 14) in the cell-free organic tissue (2; 12) through its surface (8; 18) and setting in the tissue (2; 12); wherein the said number of holes (4; 14) is formed using a needle or a kit of needles. The holes (4; 14) are partially intersected thereby forming partially connected holes (4; 14).
EFFECT: invention also refers to a respective cell-free organic tissue (2; 12) of the human or animal origin.
17 cl, 3 dwg
SUBSTANCE: what is described is a method for applying a chitosan coating on a pericardial surface of a biological heart valve prosthesis by direct chitosan application from a non-immunogenic solvent absolutely biocompatible with a human body and possessing antimicrobial properties, - high-pressure carbonated water, onto the pericardium of the biological heart valve prosthesis pre-processed with 0.625% glutaric aldehyde. The method for chitosan coating from high-pressure carbonated water enables providing higher effectiveness and prolonged functioning of the biological heart valve prosthesis ensured by avoiding formation of deposited calcium on the surface, providing better elasticity, enhancing antimicrobial properties enabled by the chitosan coating on the surface.
EFFECT: making the non-immunogenic calcification resistant biological heart valve prostheses possessing the antimicrobial properties.
12 cl, 1 dwg, 4 tbl, 12 ex
SUBSTANCE: invention refers to medicine, namely to surgery, and may be used in reconstructive surgery for performing endoscopic operations. Cadaveric dura mater tissue is prepared. The prepared dura mater is immersed into a preserving solution. Dura mater biografts are de-frozen, and the adequate biografts are cut out. The prepared grafts are packed into plastic bags and lyophilised. The prepared graft is split out to make a dura mater biograft 0.2-0.4 mm thick.
EFFECT: developing the method for making a biocompatible and thinned human dura mater biograft that makes it applicable in endoscopic operations.
4 cl, 1 tbl, 1 ex, 2 dwg
SUBSTANCE: invention refers to medicine, particularly to methods for improving the biocompatibility of valve and vascular grafts ensured by reducing the immunogenicity and preventing the calcification. The method for improving the biocompatibility of valve and vascular grafts consists in pre-incubation of the tissue grafts in normal saline containing 0.9% sodium chloride, EDTA (0.5-2 mM), organic buffer HEPES (5-20 mM) at pH 7.0 for 4-6 hours, incubation in normal saline containing 0.9% sodium chloride, HEPES 5-20 mM (pH 7.8), 1% sodium deoxycholate for 30-48 h; the tissue grafts are washed from sodium deoxycholate in a solution containing 0.9% sodium chloride, HEPES 5-20 mM (pH 7.8) and 20% ethanol for 8 days with the medium replaced for the fresh one every day and washed from ethanol for one day.
EFFECT: method for improving the biocompatibility enables preventing the graft calcification, reducing their cell toxicity and the immune response to the tissue grafts.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine and pharmacology, and represents a bioengineered collagen construct for reconstruction or replacement of damaged tissue, characterised by the fact that it comprises a layer of a purified collagen tissue matrix taken from the small intestinal submucosa, wherein said purified collagen tissue matrix is processed by a pharmaceutically acceptable antimicrobial agent and has the anti-microbial properties.
EFFECT: invention provides the bioengineered collagen construct with the improved antimicrobial properties.
17 cl, 11 ex, 2 tbl
SUBSTANCE: invention refers to medicine. What is described is a sterile dehydrated cell-free implant which when rehydrated by water or body fluids is exposed to anisotropic expansion, and can be used as a substrate for living cell adhesion, migration and growth. Collagen structures of a transplant are at least partially denatured after exposure to heat or organic solvents, such as lower aliphatic alcohols and ketones which are simultaneously preserving and sterilising agents, especially with respect to specific types of viruses. The implant is sterilised by radiation, preferentially by accelerated electrons, practically in the dehydrated condition. The transplant can be produced of various animal tissues, especially of mammalian tissues, such as human or swine tissues. Tissues suitable for the prevent invention can represent, e.g. skin, placenta, pericardium, peritoneum, intestinal wall, tendon, blood vessel, etc.
EFFECT: dehydrated implant is suitable as a temporary coating for a wound or a burn, for recovery, replacement and regeneration of tissues, and also as a substrate for cell cultivation in the laboratory conditions, and easier to flex, and being less fragile.
31 cl, 8 dwg, 6 ex
SUBSTANCE: group of inventions refers to medicine. The group of inventions consists of two objects: a method of processing and a biological tissue. The method is characterised by taking the biological tissue stabilised by glutaric dialdehyde and presented by both animal and human biological tissue, e.g. swine or bovine pericardium or human cadaver mitral valves wherein physical plasma processing, particularly of collagen tissue is performed.
EFFECT: group of inventions provides higher biocompatibility of biological tissue, cell colonisation and prolonged service life.
SUBSTANCE: what is described is a composition for an antimicrobial coating containing inorganic substance nanoparticles, an active substance, a binding agent and a solvent. As the inorganic substance, the composition contains silicone dioxide; the active substance is presented by a mixture of quaternary ammonium compound and chlorhexidine; the solvent is a mixture of ethyl cellosolve and butyl cellosolve; the binding agent is a mixture of polymethylphenylsiloxane resin and butyl/methyl methacrylate copolymer in the following proportions, wt %: butyl/methyl methacrylate copolymer 1.70-10.0, polymethylphenylsiloxane resin 5.0-20.0, silicone dioxide 0.5-3.0, chlorhexidine (20% aqueous) 3.0-8.0, quaternary ammonium compound 0.5-3.0, ethyl cellosolve 20.0-50.0, butyl cellosolve to 100%.
EFFECT: providing the water-stability of the long-action antimicrobial coating, ecological safety and applicability for porous surface finish.
4 cl, 5 tbl, 1 ex
SUBSTANCE: set task is solved by application of final slag, formed in production of ferrovanadium by alumino-silicothermic method as bactericidal material.
EFFECT: extension of raw material resources for bactericidal materials.
SUBSTANCE: method includes forming, on a surface, a coating from a dimethylalkylbenzylammonium chloride complex containing a C10-C14 alkyl group or a mixture of C8-C16 alkyl groups with a copolymer of styrene and maleic anhydride, which is subjected to preliminary chemical modification to open anhydride rings to form carboxyl groups, 50-60% of which are neutralised to form carboxylate groups, or a mixture of said complex with polyvinyl butyral, in the following ratio, wt %: antiseptic polymer complex 25-40, polyvinyl butyral 60-75.
EFFECT: full prolonged protection of articles made of aluminium alloy from fouling by microorganisms, which prevents corrosive change of the surface of said alloys.
28 ex, 3 tbl
SUBSTANCE: invention relates to means of purifying and disinfecting different types of water. Described is a disinfectant which contains a first polyhexamethylene guanidine hydrochloride component, a second alkyldimethylbenzylammonium chloride component and water, wherein content of the components in the solution is selected in the following amounts, wt %: first component 7-34.5, second component 1.75-6.9, water - the balance.
EFFECT: disinfection of drinking water.
SUBSTANCE: group of invention relates to a synergistic antimicrobial composition which includes a zinc salt of glyphosate and 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one, and is intended for controlling the level of fungi and algae. The group of inventions also provides a method of inhibiting growth or controlling growth of microorganisms in building material by adding said synergistic antimicrobial composition, a coating composition containing said synergistic antimicrobial composition and a dry film made from said coating composition.
EFFECT: use of the group of inventions increases efficiency of inhibiting growth of microorganisms, provides more precise control of the growth of microorganisms without affecting health and/or the environment.
12 cl, 7 tbl
SUBSTANCE: tableted antimicrobial composition contains polyhexamethylene guanidine hydrochloride (PHMH) in a combination with at least one ingredient specified in a group: quaternary ammonium compound (QAC), N,N-bis-(3-aminopropyl)dodecylamine and persol, as well as with an alkaline metal iodide and ammonium iodide. The antimicrobial composition has the following ratio of ingredients: PHMH - 10 to 70 wt %; QAC, N,N-bis(3-aminopropyl)dodecylamine and/or persol - 1 to 30 wt %; an alkaline metal iodide and ammonium iodide - 0.1 to 20 wt %.
EFFECT: using the invention provides the fast-acting tableted antimicrobial composition having a wide antimicrobial spectrum and a higher level of biocidal activity.
5 cl, 2 tbl, 2 ex
SUBSTANCE: invention relates to the field of poultry industry and is intended for sanation of poultry hatching eggs. The method comprises processing the egg surface with aqueous solution of preparation based on milk whey with lactulose and quaternary ammonium compound trimethyloctadecyl ammonium bromide. As the aqueous solution of the preparation based on milk whey with lactulose and quaternary ammonium compound trimethyloctadecyl ammonium bromide 0.1-0.2% aqueous solution of the preparation is used, taken in equal proportions and diluted with water in a ratio of 1:10. Hatching eggs are processed 2-4 hours prior to their laying for incubation.
EFFECT: use of claimed method enables to carry out effectively sanation of poultry hatching eggs.
1 tbl, 1 ex
SUBSTANCE: agent comprising ethanol, glycerol, Carbopol ultrese and softened water according to the invention comprises aminomethylpropanol, essential oil selected from the group: eucalyptus essential oil, lemon essential oil, peppermint essential oil, anise essential oil, jasmine essential oil with the following ratio of components in g per 100 g of gel: ethanol 65.0-66.0; glycerol 1.0-2.0; Carbopol ultrese 0.16-0.18; aminomethylpropanol 0.06-0.08; essential oil 0.005-0.03; softened water - up to 100.0.
EFFECT: invention has a high antimicrobial activity against pathogenic bacteria, fungi and viruses and provides safety for life and health of people without causing allergy.
4 cl, 4 ex
SUBSTANCE: biocidal composition for soaking napkins contains benzethonium chloride, sodium hydroxymethylglycinate, glycerine, decyl polyglucose, niacinamide, liposentol hydro, phenylmethanol and distilled water. Components are used in a defined ratio given in wt %.
EFFECT: invention improves biocidal properties of the napkin soaking composition.
3 tbl, 1 ex
SUBSTANCE: invention discloses a disinfectant and can be used in prevention and treatment facilities, at first aid facilities, disinfection facilities, child care centres, public catering facilities, public utilities, transportation, veterinary, agriculture, food industry enterprises and other sectors, as well as for pre-sterilisation cleaning of medical items, including surgical and dental instruments, rigid and flexible endoscopes and other instruments. The disinfectant contains catamine AB, isobornyl acetate, isopropanol, α-pinene, glutaric aldehyde, glycerine, a nonionic surfactant and water in defined ratios.
EFFECT: disinfectant does not cause corrosion in articles made of metal and decomposition of metals and endoscope units; does not cause organic contamination.
FIELD: process engineering.
SUBSTANCE: invention relates to treatment of fluids by cavitation. Oscillatory system with fluid and objects consists of walls each being made up of membrane secured on its edges that features intrinsic frequency equal to first harmonic, allowing for oscillator weight. Ultrasound waves are emitted by all membranes at a time to produce superposition of waves with generation of standing acoustic waves or several waves with different frequencies. Amplitude of resonance oscillation of every membrane exceeds the threshold of acoustic cavitation for fluid and objects therein. Membrane frequencies and phase characteristics are selected to mate or to differ to produce cavitation effects with due allowance for medium characteristics. Oscillatory system may have arbitrary shape, flow or stationary mode of fluid motion.
EFFECT: higher efficiency of cavitation effects.