Agent for pyoinflammatory processes in soft tissues and mucous membranes
SUBSTANCE: therapeutic agent contains an alloy of polyethylene oxide of molecular weight 400 and 1500 as a base and comprises a combination of antiseptic, benzalkonium chloride and metronidazole as therapeutic ingredients. The invention provides preparing the therapeutic agent possessing the antimicrobial, sorption and wound-healing action on local pyoinflammatory processes in soft tissues and mucous membranes, used in surgery, dermatology, obstetrics and gynaecology, otorhinolaryngology.
EFFECT: agent possesses the higher efficacy.
2 tbl, 3 ex
The invention relates to medicine, namely to funds that have antimicrobial, sorption and wound healing effect on local purulent-inflammatory processes of the soft tissues and mucous membranes, used in surgery, dermatology, obstetrics and gynecology, otorhinolaryngology.
The problem of treatment of purulent wounds is still relevant in modern medical practice. According to some authors from all surgical diseases of purulent complications account for 30-35%, and the mortality reaches 25%.
Without doubt, to achieve a positive result modern drugs should have the opposite effect and combine properties such as broad antimicrobial activity, high dehydrating ability, stimulation of tissue regeneration.
For today, the impact on the diversity of microorganisms that cause chronic inflammatory process in the soft tissues, used drugs for local application of antibiotic. However, it should be noted that the mass, and in some cases uncontrolled, their use leads to predominance in the wound are not sensitive or insensitive to them microflora. In this regard, in surgical practice in the treatment of local inflammatory processes, there has been increasing integration of the EU antiseptics and drugs.
The purpose of purulent exudate absorption and drainage of the wound surface in medicinal preparations (ointments, gels, etc. for outdoor applications currently widely used polyethylene oxides (PEO), carbon sorbents, derivatives of cellulose and other
The prototype of our invention is considered a drug, which includes (in mass fractions) of the antibiotic erythromycin estolate (1,0), antiseptics - chamomile extract (2,0) and a solution of methylene blue (0,004), and as the basis - alloy PEO-400 (79,2) and PEO-1500 (19,8), purified water (2,0) ("Experimental rationale for the use of new multi-ointment erythromycin of astolat and vibro-acoustic effects in the treatment of purulent wounds". Biryukov Century. And. Thesis Cand. the honey. n-K, 14.00.27-surgery, Kursk, 2006)
However, despite the fact that the antibiotic erythromycin is still used in clinical practice, its application is limited, as it quickly develops resistance of microorganisms, it has a relatively narrow spectrum of antimicrobial activity, instability suction, short duration of action. In turn antiseptics chamomile extract and methylene blue have not wide enough spectrum of antimicrobial action.
The technical result of the invention is to provide an effective medication the aqueous means, possessing antimicrobial, sorption and wound healing activity.
The technical result is achieved in that the means for the treatment of purulent-inflammatory processes of the soft tissues and mucous membranes contains as the base alloy oxides with a molecular weight of 400 and 1500, characterized in that as a therapeutic component contains a combination of antiseptic benzalkonium chloride and metronidazole in the following mass proportions:
|Benzalkonium chloride (Ph Eur monograf 0372)||0,02-0,04|
|Metronidazole (FS 42-2283-93)||1,0-2,0|
|The polyethylene oxide - M 400 m (FS 42-1242-96)||80,0 to 90.0|
|The polyethylene oxide - M 1500 m (FS 42-1885-96)||20,0-30,0|
Characteristic of interest:
Benzalkonium chloride (Bensalkonium chlorid). Has microbiostatic and microbicides action on gram-positive, in large doses - on gram-negative bacteria and fungi of the genus Candida. It is also known as the preservative used to prevent microbial contamination during long-term storage of dosage forms, including ointments, gels, etc. in Addition to antiseptic that has a deodorizing action is. In addition, benzalkonium chloride is a cationic surface-active agent, which can influence the degree of grinding pharmaceutical substances, thereby increasing their bioavailability and efficacy.
Metronidazole. Antibacterial chemotherapeutic drug, a derivative of 5-intorimidazole, has a high activity against a broad spectrum of bacteria and protozoa. It is proved that the anaerobes have kept him 100% sensitivity. The mechanism of action is the biochemical reduction of 5-nitro group of metronidazole intracellular transport proteins anaerobic microorganisms and protozoa. Restored 5-nitrogroup of metronidazole interacts with the DNA of the cells of microorganisms by inhibiting the synthesis of nucleic acids, which leads to the death of bacteria.
The oxides have a dehydrating effect, and in the festering wound it applies not only to its fabric, but it contains microbial cells. PEO-based dehydrates microbial cell, dramatically reduces its biological activity and weakens resistance to different drugs, resulting in significantly increased antimicrobial activity of many antibiotics, sulfonamides and antiseptics. Due to the ability of PEO to absorb exudate, and together with him and Mick is one of the toxins from the decay products of fabrics, biologically active substances of lysosomal enzymes and other mediators of the inflammatory process, "breaking" thereby its progressive course, they cause a potentiating effect on therapeutic effect of the medicinal product in General.
THE METHOD IS AS FOLLOWS.
The manufacturer of the medicinal product consists of three stages:
First stage: the calculated amount of polyethylene oxide M 1500 m (per M 1500 m) is melted in a water bath, after which it add the calculated amount of polyethylene oxide M 400 m (per M 400 m), stirred until a homogeneous mass is obtained alloy is cooled.
Second stage: the calculated number of sample metronidazole milled in the presence of the calculated amount of benzalkonium chloride. Third stage: powdered mixture of metronidazole and benzalkonium chloride in parts add alloy PEO M 1500 m and PEO M 400 m and homogenized to obtain a homogeneous mass.
EXAMPLE 1 (METHOD GET)
20,0 mass fractions of PEO M m 1500 melted on a water bath, then add in parts 80,0 mass fractions of PEO M 400 m and stirred until a homogeneous mass is obtained alloy leave to cool. Then crush the sample to 1.0 mass fraction of metronidazole in the presence of 0.02 mass fraction benzalkonium chloride. After that, the obtained sm is si metronidazole and benzalkonium chloride in parts add alloy oxides (PEO M m 400 and PEO M 1500 m) and homogenized to obtain a homogeneous mass.
EXAMPLE 2 (METHOD GET)
30,0 mass fractions of PEO M m 1500 melted on a water bath, then add in parts 90,0 mass fractions of PEO M 400 m and stirred until a homogeneous mass is obtained alloy leave to cool. Then crush the sample to 2.0 mass fraction of metronidazole in the presence of 0.04 mass fraction benzalkonium chloride. Then to the resulting mixture metronidazole and benzalkonium chloride in parts add alloy oxides (PEO M 400 m and PEO M 1500 m) and homogenized to obtain a homogeneous mass.
DETERMINATION of MICROBIOLOGICAL ACTIVITY FUNDS FOR the treatment of PURULENT-INFLAMMATORY PROCESSES of the SOFT TISSUES AND MUCOUS MEMBRANES
Antimicrobial activity funds for the treatment of purulent-inflammatory processes of the soft tissues and mucous membranes was studied by the method of diffusion in agar (GF XII, 2007) against the test strains St. aureus ADS 6538-P, You. cereus was ATSS 10702, E. coli ATCC 25922, Proteus vulgaris and Pseudomonas aeruginosa was ATSS 9027, Candida albicans was ATSS 885-653. The determination results are presented in table.1.
From the data presented in table.1, it follows that the remedy for the treatment of purulent-inflammatory processes of the soft tissues and mucous membranes (example No. 1 and No. 2) has a strong enough b is oldnum action against all three test strains. The zone of inhibition of growth when studying the spectrum of antimicrobial activity is most pronounced against the test strains St. aureus ATCC 653 8-P, You. cereus ATCC 10702, Candida albicans ATCC 885-653, E. coli ATCC 25922, Proteus vulgaris, which is not inferior, and in some ways even surpasses zone of stunting in the study of the prototype (erythromycin estolate, chamomile extract, a solution of methylene blue alloy PEO M 400 m and 1500).
EXAMPLE 3 (EXPERIMENTAL USE)
Study of wound healing activity funds for the treatment of purulent-inflammatory processes of the soft tissues and mucous membranes (example No. 1 and No. 2) were carried out in the experiment on rats male breed "Wistar" weighing 180-200 g, which was modeled purulent wound by the method of P. I. Tolstykh (1976). Experimental animals were divided into three sets of 36 animals in each:
In 1 series (model) treatment was not conducted.
In the 2nd series (control) used for the treatment of ointment erythromycin estolate, chamomile extract, a solution of methylene blue, alloy PEO M 400 m and 1500 (prototype).
In the 3rd series treated tool for the treatment of purulent-inflammatory processes of the soft tissues and mucous membranes (sample No. 1).
In the 4th series treated tool for the treatment of purulent-inflammatory processes of the soft tissues and mucous membranes (example No. 2).
The course of the wound process in the experiment, the selected animals were evaluated planimetric method (L. I. Popova), microbiological, histological methods. The research results are processed statistically (calculated averages, criterion validity).
The animals were sacrificed experiment by overdose of ether anesthesia on 1, 3, 5, 8, 10, 15 day.
After modeling on the 1st day in all the series was marked hyperemia edges of the wound, swelling of the surrounding tissues, the bottom of the wounds were covered with fibrin with areas of necrosis, purulent discharge was observed.
In the course of treatment was cleansing the wound from purulent-necrotic masses, were stopped swelling, appeared granulation and marginal epithelization.
Data planimetric studies indicate a more rapid decrease in the area of RAS in the 3rd and 4th series compared with model control (the original area of the wounds was 250 mm2), with 3 days square RAS had statistically significant differences. The healing process proceeded faster in the 3rd and 4th series compared to the control and the model. The planimetric results of the study are presented in table.2.
Thus, the data obtained planimetric studies confirm the high effectiveness of the drug for treatment of purulent-inflammatory processes of the soft tissues and mucous membranes (examples 1 and 2) in the treatment of purulent wounds. To the 15th day a t the d ' wounds in the 3rd and 4th series decreased by 98,3%, that was higher than the speed of wound healing in an average of 2.8 times in comparison with the control series.
These microbiological studies have shown that in the 3rd and 4th series of microbial infection of wounds is significantly less than in the control series, starting with the 5-day and throughout the observation period. In the 3rd and 4th series 5th day microbial colonization of RAS 2.2 times less than in the control series (64,1×2,82×105, 57,3±3,17×105and 1.4±0,21×107CFU/g, respectively), on the 10th day - 4.1 times less than in the control series (18,9±1,54×103, 16,1±2,21×103and 7.6±0,93×104CFU/g, respectively). It was thus established that the remedy for the treatment of purulent-inflammatory processes of the soft tissues and mucous membranes (examples 1 and 2) 2.2-4.1 times reduces microbial colonization of wounds in comparison with the prototype - ointment containing erythromycin estolate, chamomile extract, a solution of methylene blue, alloy PEO M 400 m and 1500.
To fully assess the condition of the wound in the dynamics was used histological research method of wound biopsies, giving the opportunity to objectively assess the dynamics of the course of wound process.
On the 1st day after modeling the microscopic picture in all the series is as follows. The surface of the wound covered in a thick fibrinous-leukocyte layer. Leukocytes are able RAS is hell. Connective tissue under leukocyte scab swollen, infiltrated isolated macrophages and segmented by leukocytes. Connective tissue loosened fibroblasts have basophilic cytoplasm distended and swollen core. There are foci of hemorrhage diapedetic character. The epithelium of distriict, the edge of his flat.
On the 3rd day in a group of animals in the control series on the surface of the wound - wide fibrinous-leukocyte scab under which there is active growth of granulation tissue. Granulation tissue abundantly infiltrated by leukocytes. Fibroblasts swollen with basophilic cytoplasm are heavyweights. Intercellular substance swollen, distended. On the 10th day of leukocyte-necrotic scab is completely separated from the surface of the wound. In the surface layer of the wound is determined fibroblasts and numerous macrophages. Infiltration is expressed weaker than on the previous day of observation. On the surface of the granulations continued growth of the epithelium. On the 15th day wound on the large surface epithelization. Beneath the epithelium is the connective tissue layered structure containing collagen fibers and infiltrated by leukocytes and macrophages. Swollen intercellular substance.
On the day 3 when using tools for the treatment of inflammatory Pro is escow soft tissues and mucous membranes (examples 1 and 2) there is a slight infiltration of fresh granulation by leukocytes. On the 10th day is the restoration of the skin (creeping epithelial shaft with the edges of the wound). On the 15th day wound epithelization, was observed almost complete recovery of the skin except its derivatives.
Thus, in the 3rd and 4th series notes faster cleaning of the surface of the wound from leukocyte-necrotic masses, active growth of granulations. By the 10th day, a significant portion of the wound or all of its surface epitelizirutmi.
Apply the remedy for the treatment of purulent-inflammatory processes of the soft tissues and mucous membranes (example 1 and 2) in the first and second phases of the wound healing process speeds up healing of wounds 2.8 times, 2.2 -4,1 times reduces microbial colonization of the Russian Academy of Sciences, accelerates the formation and maturation of granulation tissue, promotes early and rapid epithelialization of the wound surface in comparison with treatment with an ointment containing erythromycin estolate, chamomile extract, a solution of methylene blue, alloy PEO M 400 m and 1500 (the prototype of the invention).
For the treatment of purulent-inflammatory processes of the soft tissues and mucous membranes, containing as the base alloy oxides with a molecular weight of 400 and 1500, and includes as a therapeutic component combination antiseptic benzalkonium chloride and metronidazole in the following mass proportions:
|Polyethyleneoxide M 400 m||80,0 to 90.0|
|Polyethyleneoxide M 1500 m||20,0-30,0|
SUBSTANCE: invention relates to application of 2-R1-4-R2-6-polynitromethyl-1,3,5-triazines of general formula: , where n=0, X=NO2, Cl, Br, R1=R2=OR3, OAr (R3=CH3, C2H5, CH2(CH2)6CH3, CH2CH2Cl, Ar=metha-C6H4CH3), R1=OR3, OAr, R2=N(C2H5)2; n=1, X=Cl, R1=OR3, R2=NH(CH2)2NH2, N(CH2CH2)2NCH3 as compounds, which possess antibacterial activity.
EFFECT: identification of compounds based on 1,3,5-triazine derivatives, which possess high antibacterial activity.
3 tbl, 7 ex
SUBSTANCE: method of producing a carbon sorbent with antibacterial and antimycotic properties includes impregnating granules of a carbon hemosorbent with 10-50% aqueous solution of glycolic acid for 7-9 hours at room temperature. The ratio of the hemosorbent to the modifier solution of glycolic acid is 1:1. Further, drying is carried out for an hour at 100-110°C. Polycondensation of glycolic acid on the carbon hemosorbent is carried out in two steps: at 185-205°C for 1 hour, at 215-235°C for not less than 5 hours, on a sand bath. The disclosed modified carbon sorbent with antibacterial and antimycotic properties is granules with a round shape, contains not less than 5% polyglycolide, characterised by a specific adsorption surface area of less than 250 m2/g and total pore volume of less than 0.50 cm3/g.
EFFECT: improved properties of the sorbent.
2 cl, 2 tbl, 2 dwg
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to compounds of formula and their pharmaceutically acceptable salts possessing antibacterial properties, as well as to a based pharmaceutical composition. In formula (I) R1 represents an alkoxy group; each of U and V represents CH, and W represents N, or U represents N, V represents CH and W represents CH or N, or each of U and V represents N, and W represents CH; R2 represents hydrogen or fluorine, if W represents CH, or R2 represents hydrogen, if W represents N; A represents O or CH2; Y represents CH or N; Q represents O or S; and n represents 0 or 1.
EFFECT: preparing the pharmaceutically acceptable salts possessing the antibacterial properties.
23 cl, 3 tbl, 15 ex
SUBSTANCE: invention refers to medicine, namely to surgery, and concerns preventing the abdominal adhesion formation following the intra-abdominal operations. To this effect, a laparotomic wound closure is preceded by administering Miramistin 3 ml/kg of body weight into the abdominal cavity. The preparation is left in the abdominal cavity for 5 hours and passively evacuated.
EFFECT: preparation dose schedule provides the effective prevention of the adhesion formation by the antiadhesive, antiseptic and microcirculation improving action of Miramistin.
FIELD: veterinary medicine.
SUBSTANCE: method comprises administering a mixture of medicinal solution of formaldehyde and isotonic solution of sodium chloride at a ratio by weight of (2-6):(994-998). The mixture is administered intramuscularly at a dose of 5-6 ml 6 times. The second injection is administered on the day 7-8 after the first injection, the third - on the day 7-8 after the second injection, the fourth - 12 hours after the third, the fifth - 12 hours after the fourth, the sixth - 7-8 days after the fifth injection.
EFFECT: method is highly effective in treatment of brucellosis of cattle.
3 tbl, 3 ex
SUBSTANCE: present invention relates to a preparation which inhibits microbial growth, which includes an arylamide compound as an active compound and kleptose or captisol.
EFFECT: methods of producing the preparation, use and method of treating microbial infections are disclosed.
21 cl, 5 dwg, 11 tbl, 16 ex
FIELD: veterinary medicine.
SUBSTANCE: pharmaceutical composition in the form of ointments for treatment of mastitis in cows comprises n-tetradecyltributylphosphonium bromide as active ingredient and petrolatum as an excipient at the ratio of 1:2000.
EFFECT: invention provides a pharmaceutical composition in the form of an ointment, which is new in mechanism of action, effective when applied topically at low therapeutic concentration.
SUBSTANCE: invention relates to novel hexasubstituted para-aminophenols: where R=C6H5, C6H4Cl, C6H4Br, C6H4CH3, with arylamide groups in 2,6 positions with respect to hydroxyl, which demonstrate antibacterial activity.
EFFECT: hexasubstituted para-aminophenols with arylamide groups in 2,6 positions with respect to hydroxyl, possessing high bactericidal and bacteriostatic activity.
1 tbl, 4 ex
SUBSTANCE: invention relates to veterinary, in particular to obstetrics, gynecology and reproduction biotechnology, and can be used for treating animals in case of clinical mastitis. Medication for treating animals with clinical mastitis includes: trivit - 10.0 ml, cefotaximum - 750 mg, prednisolone - 10.0 mg, nystatin - 325.0 ml. Medication is introduced to ill animals intracycternally in dose 10.0 ml with interval 12 hours after milking.
EFFECT: invention provides increased efficiency of treating clinical mastitis by complex impact on all sides of pathological process.
4 tbl, 3 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine, more specifically to new dosage forms of cephem compounds effective for treating bacterial infections.
EFFECT: dosage forms are stable; they show the improved solubility and are especially applicable for parenteral administration.
9 cl, 29 tbl, 8 ex
FIELD: veterinary medicine.
SUBSTANCE: preparation for treatment of infected wounds in the toe region of animals, containing dimethyl sulphoxide, methyl cellulose, additionally comprises tylosin tartrate, sulphadimine, trimethoprim in the following ratio of components, wt %: tylosin tartrate - 2-3, sulphadimine - 4-5, trimethoprim - 1-1.5, dimethyl sulphoxide - 10-15, methylcellulose - 3.0-3.5, water - the rest. The claimed method comprises applying the claimed preparation without preliminary wound cleaning. The preparation is applied to the wound with the layer thickness of 2-3 mm, without application of soft dressing. The treated animals are kept for 2-3 hours on the dry surface.
EFFECT: use of claimed group of inventions increases the efficiency of treatment.
4 cl, 7 tbl, 2 ex
SUBSTANCE: invention refers to medicine, namely to surgery, and can be used for treating trophic ulcers and purulonecrotic involvements of lower extremities in diabetic patients. That requires a baseline therapy and a regional fibrinolytic therapy. Conducting the regional fibrinolytic therapy following applying a rubber bandage on the lower one-third of the shin is accompanied by administering Urokinase medac in a dose of 100 thousand units into the heel bone once a day within 5 days.
EFFECT: in the setting of reducing the total dosage of the preparation, the invention enables providing the high concentration of Urokinase medac in a pathological centre, improving microcirculation and metabolic processes in the involved tissues, accelerating the wound healing and reducing the length of hospital admission of the patients suffering from diabetic foot syndrome.
SUBSTANCE: pharmacological composition contains a therapeutic agent and a pharmaceutically acceptable base. As a therapeutic agent, it contains recombinant interferon specified in a group: recombinant interferon alpha, recombinant interferon beta, recombinant interferon gamma, as well as hypromellose, boric acid as an antiseptic, anesthesin or lidocaine as local anaesthetics in the following proportions, g per 1 ml of the mixture: recombinant interferon, IU 100-10,000,000, hypromellose 0.00001-0.5, boric acid 0.00001-0.5, anesthesin or lidocaine 0.00001-0.5, a pharmaceutically acceptable base - the rest. Besides, the pharmaceutical composition contains heparin in an amount of 0.00001-0.5 g; antibiotics specified in a group of: baneocin, levomycin, tetracycline, amoxicilline in an amount of 0.00001-0.5 g. And as a pharmaceutically acceptable base, the pharmaceutical composition contains macrogol 400, or macrogol 1500, or macrogol 4000.
EFFECT: more effective treatment.
4 cl, 9 ex
SUBSTANCE: ulcer region is coated with macroporous hydrogel with the concentration of lightly cross-linked acrylic polymer 0.2-0.6 wt %, gel viscosity 45-85 poises at pH from 7.0 to 7.8. The wound floor is coated with a layer 2-4mm thick, and the wound edges - 1.5-3 mm, while a periulcerous region - 1-3 mm thick.
EFFECT: healing trophic ulcer 5-15 days later and no recurrence for 1-2 years as shown by patient's follow-up.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to new idebenone derivatives substituted by carboxylic acid with general formula I wherein R1 represents C2-C22 saccharic acid with a direct or branched chain, while two or more hydroxy groups are independently substituted by C1-C22 carboxylic acid, wherein the term 'branched' refers to one or more groups of a lower alkyl. The invention also refers to a formulation for skin treatment containing the above idebenone derivatives, a method of treating the skin changes by local applications with these idebenone derivatives, as well as to methods of synthesis thereof. When adding the idebenone derivatives according to the present invention into the formulations for local application, they possess the antioxidant action effective in treating the skin changes.
EFFECT: above idebenone derivatives have unexpectedly occurred to be effective in skin treatment, especially in relation to skin tolerance.
20 cl, 1 tbl, 5 ex
SUBSTANCE: method comprises scaling of diploid cells of line M-20 from cryobank of Institute of Poliomyelitis and Viral Encephalitis n.a. MP Chumakov of RAMS from the ampoule of the seed cell bank of 7 passage with obtaining the working cell bank of 16 passage. At that the cells of 20-33 passages, suitable for use in therapeutic and/or diagnostic purposes are produced by culturing in a nutrient medium containing 10% of human fibrinolytic active plasma (FAP), comprising platelet-derived growth factor PDGF in a concentration of from 155 to 342 pg/ml.
EFFECT: invention enables to increase proliferative activity of human fibroblast diploid cells.
2 cl, 2 tbl
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to pharmaceutical industry and represents dermatological cream, intended for local treatment of bacterial skin infections and for healing wounds associated with them, which contains framycetin sulfate and biopolymer, included into cream base, which contains at least one substance from each of the following groups: preservative, primary and secondary emulsifier, selected from the group which contains ketostearyl alcohol, ketomacrogol 1000, polysorbate-80 and Span-80; paraffin as wax-like product, cosolvent, selected from the group, including propylene glycol, hexylene glycol and polyethylene glycol-400; nitric acid or lactic acid and water, with said biopolymer preferably being chitosan.
EFFECT: invention provides higher therapeutic effect.
8 cl, 10 tbl, 2 dwg
FIELD: medicine, pharmaceutics.
SUBSTANCE: present invention refers to medicine, particularly to aspects covering antimicrobial compositions, and described antimicrobial compositions, antimicrobial silicone gel based on the above antimicrobial composition, a wound dressing and methods for preparing them. Among other things, the antimicrobial compositions contain at least one alkenyl- and/or alkynyl-substituted polysiloxane, at least one polysiloxane containing silicone-linked hydrogen atoms, and at least one hydroxylation catalyst, at least one hydrophilic ingredient, at least one silver salt.
EFFECT: invention can be used for preparing a drug preparation to be used in treating burns, scars, bacterial infections, viral infections and/or mycotic infections.
19 cl, 5 dwg, 8 ex, 6 tbl
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to a topical pharmaceutical preparation for treating inflammatory skin pathologies, preferentially an ischemia-related skin pathology. The above preparation contains a supernatant of normal saline prepared by culturing for at least 1 hour peripheral blood mononuclear cells (PBMC) or their subpopulation in normal saline free from substances promoting the PBMC proliferation and activating the PBMC in the stress-inducing environment. Also, the invention refers to a method for producing and using the above preparation.
EFFECT: invention promotes relieving the symptoms of the inflammatory skin pathologies and the healing process.
20 cl, 18 dwg, 5 ex
SUBSTANCE: biocompatible polymer representing a mixture of Pluronic F127 and carboxymethyl cellulose sodium salt is immobilised on hydroxyapatite nanoparticles. The agent represents the following mixture ratio, wt %: dimethylglucamine salt of chlorine E6 - 0.03-0.45; hydroxyapatite - 0.1-0.3; Pluronic F127 - 2.0-2.5; carboxymethyl cellulose sodium salt - 2.0-2.5; water - the rest. What is also presented is a method of preparing the above agent by immobilising the biocompatible polymer with an included dimethylglucamine salt of chlorine E6 on hydroxyapatite nanoparticles, involving mixing in water of Pluronic F127 with dimethylglucamine salt of chlorine E6, agitating the prepared mixture and solution for 30-40 min and adding carboxymethyl cellulose sodium salt. What is also presented is a method of treating septic wounds of soft tissues combining conventional treatment according to a standard practice and photodynamic therapy. The photodynamic therapy involves coating a wound surface with the presented agent in an amount of 0.05-0.1 g per 1 cm2 of the wound surface, settling for 40-60 min; with the agent coating kept on, the wound surface is exposed to a low-intensity laser light at a wavelength of 663±0.03 nm, power density 1 Wt/cm2 and energy density 25-30 J/cm2.
EFFECT: invention provides the high clinical effectiveness.
8 cl, 2 dwg, 7 ex
SUBSTANCE: first, the method involves teeth cleaning, surgical site isolation with cotton swabs, drug-induced treatment of 2% chlorhexidine and drying. Then, an erosion surface and adjoining solid dental tissues are coated with a phytoapipreparation for 15-20 minutes. The preparation is presented in the form of an ointment and has the following composition, weight fractions: 40% alcoholate (1:10) of tartarian catchfly or rhizomes and roots of maral root 10 ml, 40% alcoholate (1:10) of spirea herb 5 ml, apilac 0.5 g, clove ester 0.5 ml, lanolin 25.2 g, Vaselin 58.8 g. The ointment is used once a day for 10 days with the patients advised to avoid eating for one hour.
EFFECT: method is easy-to-use and physiological, and ensures an absolute recovery.