Method of determining necessity in sanitary-hygienic processing of livestock buildings
FIELD: veterinary medicine.
SUBSTANCE: strip of filter paper with the width of 20 mm is attached on the filter, mounted in the ventilation system of the test livestock building, for a period of 4 hours. 1% sterile glucose solution is prepared on physiological solution, which is used as a nutrient medium. The strip of filter paper is placed in 10 ml solution of glucose and thermostated at the temperature 37±1°C for 2 hours. Then the electric conductivity of the solution is measured. The conclusion about the necessity in sanitary-hygienic processing of livestock buildings is made when the electric conductivity value is 287.3 mcS/cm, which corresponds to maximum allowable concentration of microorganisms in the air of the working area or below.
EFFECT: invention enables to determine reliably the microbiological environment in the buildings and to make the prompt conclusion about the necessity of sanitary-hygienic processing of livestock buildings.
The invention relates to the field of assessment of microbiological environment environment and may find application in the field of agriculture, characterized by high bacterial colonization, such as in livestock and poultry premises.
Control of microbiological condition of the premises is necessary, first, because of the influence of microorganisms on human health and, secondly, because of their ability to biodegradation of various materials.
There is a method of assessing the microbiological situation in residential pressurized space object, namely, that take samples of air in the internal volume of the housing pressurized, deliver samples to Earth, sown sample on a nutrient medium, analyze visual media composition and number of individual components of the microflora, the results of the analysis are judged on the state of the microbiological environment in residential pressurized space object. As a sample use content filters installed in the ventilation system and manual vacuum space object (RF patent 2308291, publ. 20.10.2007) .
The disadvantages of this method are the complexity associated with the dismantling of the filter and the extraction of microorganisms, the duration of the analysis.
The closest in technical essence and the achieved financial p the tattoo is a way of determining the number of microorganisms in the air, namely, that the microbial aerosol precipitated on a nutrient medium (1% sterile glucose solution), followed by temperature control, and the number of microorganisms is determined by the conductivity temperature-controlled trial on schedule empirical dependence (the decision to grant patent for invention dated 04.04.2013, application No. 2012111034) .
The disadvantage of this method is the locality of sampling.
The objective of the invention is to increase the reliability assessment of the microbiological environment and operational conclusion about the necessity of sanitary and hygienic processing of livestock premises.
Technical challenge is sampling the air environment around the building volume.
The problem is solved due to the fact that in the known method of determining the necessary sanitary and hygienic processing of livestock premises, namely, that precipitated the microbial aerosol from the air, the resulting sample thermostatic at a temperature of 37±1°C in 1% sterile glucose solution, and then measure the conductivity of the resulting solution, according to the invention, the microbial aerosol precipitated on a strip of filter paper, made of 20 mm and fixed for 4 hours in the filter installed in the ventilation of livestock buildings, if this is the conclusion about the necessity of sanitary and hygienic processing of livestock premises do when the conductivity value 287,3 µs/cm, the corresponding MPC microorganisms in the air of working zone or below.
The essence of the proposed solution is illustrated by a drawing, which shows a graph of the number of microorganisms detained strip of filter paper attached to the filter, depending on the exposure time.
The implementation of the method consists in the following. The filter installed in the ventilation system studied livestock premises, secure the strip of filter paper with a width of 20 mm for a period of 4 hours. The proposed width of a strip of filter paper allows not to interfere with the operation of the filter. The length of the strips depends on the size of the filter. Thanks to the circulation of air through the filters, the microorganisms present throughout the volume of the room, trapped by the material of the filter paper.
Based on the data, for 4 hours on the strip precipitates the main part of the microorganisms present in the air. A further increase in exposure leads to a slight increase in the number and impractical.
In addition, filter paper provides detention mechanical particles mainly on its surface. Materials surface filtration ensure the stability of the percentage of the detention of particles of a given size, but have a small life. This is because what is driving available through pores filtered particles mainly at the entrance to them, which leads to a decline in the area of the filter.
Next, a strip of filter paper is removed from the filter and cut into samples with a length of 40 mm, This length is due to the convenience of the premises of the obtained sample in a test tube.
Prepare a 1% sterile solution of glucose at physiological solution, which is used as the nutrient medium. Sample strips of filter paper are placed in a test tube with 10 ml of glucose solution and thermostatic at a temperature of 37±1°C within 2 hours.
Then measure the conductivity of the solution obtained using the sensor KDS-1038. According to GOST SSBT 12.1.005-88 General sanitary-hygienic requirements to the air of the working zone of maximum permissible concentration (MPC) in the air of working zone of microbial aerosol livestock and poultry premises - 50,000 cells in m3(Annex 2, p. 171). Experiments have shown that this value of microbial aerosol corresponds to the conductivity of the resulting solution, equal 287,3 µs/see the Conclusion about the necessity of sanitary and hygienic processing of livestock premises do when the conductivity value corresponding to the maximum permissible concentration of microorganisms in the air of working zone or below. The greater the number of microorganisms, the lower the conductivity.
The proposed method can reliably determine microbiologists is a mini-environment in the premises and give a prompt conclusion about the necessity of sanitary and hygienic processing of livestock premises.
How to determine the necessary sanitary and hygienic processing of livestock premises, characterized in that the microbial aerosol from the air precipitated on a strip of filter paper, made of 20 mm and fixed for 4 hours in the filter installed in the ventilation of livestock buildings, the resulting sample thermostatic at a temperature of 37±1°C for 2 h in 10 ml of 1% sterile glucose solution, and then measure the conductivity of the resulting solution, and the conclusion about the necessity of sanitary and hygienic processing of livestock premises do when the conductivity value 287,3 µs/cm, corresponding to the maximum allowable concentration of microorganisms in the air of working zone or below.
FIELD: measurement technology.
SUBSTANCE: invention refers to measurement equipment and can be applied in determination of electric parameters of space. Method involves placement of probe in the form of open flat capacitor screened from solar radiation by opaque screen, in space and feed of HF signals of definite frequency to the probe. Measurement data are obtained from the probe in the form of loss angle tangent and dielectric permeability of medium under examination, allowing for determination of attenuation rate per length unit for space.
EFFECT: possible determination of attenuation rate per length unit for space.
FIELD: measurement equipment.
SUBSTANCE: device for determining parameters of metal-dielectric structures, which contains a wave-guiding system connected to a cylindrical resonator, in the hole of which a coupling element is arranged, differs by the fact that the coupling element is of measurement type and made in the form of an adjustable quarter-wave frame, one end of which is connected to the housing of the cylindrical resonator, and the other one is made in the form of a nib point placed into a dielectric insert arranged in the holes of the cylindrical resonator housing and projecting beyond external boundaries of the resonator by the value that is smaller than length of a standing electromagnetic wave of the main type of the cylindrical resonator; the device includes additional coupling element intended for input/output of electromagnetic radiation of microwave range, which is made in the form of two metal quarter-wave frames connected to each other with one end and placed into a dielectric insert located in the holes between the wave-guiding system and the cylindrical resonator housing. The first frame is made so that its plane can be turned and the same frame is located in the inner cavity of the cylindrical resonator, and the second one is located in the wave-guiding system; other ends of frames are connected to the cylindrical resonator housing and the wave-guiding systems accordingly; in the wave-guiding system an arrangement is made for a one-dimensional waveguide microwave photonic crystal representing periodically alternating layers of two types; layers of the first type have constant value of relative dielectric permeability that is higher than one, and layers of the second type - close to one; total number of layers and number of layers of the second type - uneven; layers of the first type are extreme in the photonic crystal structure; thickness of layers of the first type is less than thickness of layers of the second type; sum of electrical lengths of layers of the first and the second types is equal to half length of an electromagnetic wave, which corresponds to the middle of the used frequency range; thickness of central layer of photonic crystal comprises one fourth of thickness of the layer of the second type.
EFFECT: improving sensitivity and enlarging functional capabilities.
FIELD: measurement equipment.
SUBSTANCE: method involves force action on the surface of a test object, recording of a set of electrical signals of input information with the information sensors installed on the test object; signals of the information sensors are determined with changes in force action on the surface of the test object. A use is made of information sensors installed on the test object and receiving changes in parameters of the test object, electronic filters to increase signal-to-noise ratio, which are connected to the outputs of the sensors and connected to the inputs of an electronic analogue circuit implementing a neural network model of reliable operation of the test object; with that, to another group of inputs of the electronic analogue circuit there connected is a unit for supply of a signal for retraining of a model with wears, and to its output there in-series connected is approximation unit of time dependences of sets of electrical signals, a shaping unit of time series of forecasts of reliable operation of the test object, an extrapolation unit of the value of the set of electrical signals at the output of the electronic analogue circuit till a limit value and determination of residual life, to the input of which an extrapolation model setting unit is connected.
EFFECT: improving reliability of forecast results; improving flexibility of the method and enlarging its application scope.
3 cl, 3 dwg
FIELD: measurement equipment.
SUBSTANCE: system of localised control of combustible gas leaks by primary parameters of measuring devices includes stationary sensors-gas analysers of combustible gases, an automatic control system, comprising a unit of sound and light alarm, a unit of control of sensors-gas analysers. The system additionally includes a unit of automatic switching of gas supply from the main process line into a reserve one and back, air-conducting boxes with a common swirler, where air is injected with required parameters from an air blowing plant, making it possible to move a gas leak in a certain direction to serially arranged sensors-gas analysers, which will make it possible with a sufficient extent of accuracy to determine local arrangement of the available leak in the shortest possible time from the moment of its formation.
EFFECT: increased safety, timely, efficient and effective detection of a local leak point, reduced risk of gas concentration formation in the air.
FIELD: testing equipment.
SUBSTANCE: invention relates to the field of non-destructive control of materials and substances, measurement of statistic characteristics of random processes. A device to control materials and substances comprises serially connected a source of physical field, an element with a control object, a converter of physical field, and also the first and second conversion circuit, each of which comprises serially connected an accumulating averaging summator and a count block, at the same time the outlet of the first circuit is connected to the first inlet of a computing device, and the outlet of the second circuit is connected to the second inlet of the computing device, the first outlet of which is connected to inlets of strobing of accumulating averaging summators combined into a bus "Measurement time", the first multiplier, the first analogue-digital converter and the second analogue-digital converter, the second multiplier, the first controlled frequency multiplier, serially connected the second controlled frequency multiplier and the controlled phase changer, the outlet of which is connected to the second inlet of the second multiplier, the outlet of which is connected to the inlet of the second analogue-digital converter, and the first inlet of the multiplier is combined with the first inlet of the first multiplier and is connected to the outlet of the first controlled frequency multiplier, the inlet of which is connected to the outlet of the physical field converter, and the outlet of the source of physical field is connected to the inlet of the second controlled frequency multiplier, the outlet of which is connected to the second inlet of the first multiplier, the outlet of which is connected to the inlet of the first analogue-digital converter, in which the outlet is connected to the inlet of the first circuit of conversion, and the inlet of the second conversion circuit is connected to the outlet of the second analogue-digital converter, besides, control inlets of the first and second controlled frequency multiplier and controlled phase changer are combined into a bus "Plant Vm" and are connected to the second outlet of the computing device.
EFFECT: simplification of a circuit of a device for control of quality of materials and substances.
SUBSTANCE: invention relates to facilities for monitoring of injury treatment process. A monitoring device comprises a unit of injury nitrogen oxide level monitoring, a unit of controlling signal generation by means of comparison of a nitrogen oxide level with preset threshold and unit of correction of light dosing for injury treatment, at the same time the monitoring unit is designed to detect magnetic field produced as a result of transition from Fe2+ into Fe3+, production of Fe3+ level in accordance with magnetic field, calculation of met Hb level in accordance with the level of Fe3+ and calculation of nitrogen oxide level in accordance with proportionate ratio between the level of met Hb and level of nitrogen oxide. The injury treatment device comprises several sources of light and a monitoring device.
EFFECT: using this invention makes it possible to correct treatment dose more accurately and conveniently, with minimum side effects.
7 cl, 4 dwg
SUBSTANCE: invention relates to means of efficient detection of poisoning substances and toxins and their instant neutralisation. Device contains microprocessor sets of first 16 and second 22 order, unit of template memory 17, units for detection of poisoning substances and toxins, audio-video system, and units of detection of poisoning substances and toxins are made in form of absorbing devices 3-7, which have sensors at outlet, which determine level of air environment contamination, outlets of sensors are connected to amplifiers-converters 11-15, connected with outlets-inlets with microprocessor set of first order 16, which is connected by outlets-inlets to unit of template memory 17, unit of question introduction 18 and microprocessor set of second order 22, unit of template memory 17 is connected by inlets-outlets to matrix field 21 in form of based on liquid crystals diode crystal lattice, unit of question introduction 18 is connected by inlets-outlets with unit of response analysis 19 and unit of analysis of unknown chemical compounds and combinations of poisoning substances 20, which is connected by inlets-outlets to unit of response analysis and to matrix field 21, connected with inlets-outlets of unit of question introduction 18 and to microprocessor set of second order 22, connected by inlets-outlets with hazard warning unit 23, unit of analysis of unknown chemical compounds and combinations of poisoning substances 20, matrix field 21 and unit of executive device 24 for neutralisation of poisoning substances and toxins, which is connected by outlets with executive mechanisms 25-27.
EFFECT: possibility of determining concentration of toxic materials and their neutralisation in the shortest terms and instant warning of people about hazard, ie maximally possible protection of people against impact of different toxins.
SUBSTANCE: method for determination of pipeline technical condition consists in technical condition integral characteristic quantitative evaluation from which pipeline state is evaluated and corresponding corrective measures are planned. To determine mentioned integral characteristic in-line inspection (ILI) and integrated corrosive survey of pipeline is performed. From results of ILI, proportion factor between technical condition characteristic and relative quantity of defective pipes is established depending on pipeline diameter. Pipeline integrated corrosive survey is performed by measuring electric current from external source along pipeline route with interval not exceeding 10 m. According to obtained data, proportion factor between relative quantity of defective pipes and relative length of damaged protective coating. From determined parameters, technical condition integral characteristic is determined which describes pipeline damage.
EFFECT: higher quality of pipeline reconstruction, repair and technical diagnosis planning.
1 tbl, 2 dwg
SUBSTANCE: bombardment of a surface with an ion beam and recording of intensity of reflected ions is performed; besides, analysed surface is bombarded with inert gas ions with energy of less than 100 eV, and energy spectrum of reflected ions is recorded in the energy range, which is higher than energy of primary ions; then, as per energies of peaks of pair collision in the obtained spectrum there determined are types of atoms in one upper monolayer of atoms, as per the available peak with energy equal to energy of bombarding ions there evaluated is availability of a crystalline phase on amorphous or amorphised surface, including in a film of nanodimensional thickness, and as per the ratio of values of the above peak without any energy losses to a peak or peaks of pair collision there determined is surface concentration of crystalline phase on amorphous or amorphised surface.
EFFECT: reduction of depth of an analysed layer till sub-nanodimensional values; improvement of reliability of analysis results and enhancement of compatibility of equipment for implementation of the method with other analysis methods and process equipment.
FIELD: measurement equipment.
SUBSTANCE: use: to measure change in the local concentration of impurities in the liquid flow at the entrance to the measuring cell. The substance is that the first change of impurity concentration is determined in time within the measurement cell to the fluid containing an impurity, wherein the concentration changes with time at the entrance of the measuring cell is known, and they find the impulse response of the measuring cell using the deconvolution method. Then they determine the change in the impurity concentration within the measuring cell for the sample liquid with an unknown impurity concentration at the entrance. They calculate the unknown concentration of impurities upon entering the measuring cell using the found impulse response of the measuring cell and a definite change in the impurity concentration inside the cell.
EFFECT: improving the accuracy of determining concentration of impurities without changing configuration of a measuring cell.
8 cl, 5 dwg
SUBSTANCE: invention proposes a method for obtaining a standard specimen of turbidity of bacterial suspensions, a standard specimen of turbidity of bacterial suspensions, use of the standard specimen of turbidity of bacterial suspensions, as well as a set containing the standard specimen of turbidity of bacterial suspensions. The method involves shuttling of chemically neutral borosilicate glass without any carbon dioxide bubbling in presence of thiomersal during 10-70 days. Shuttling is performed in a shaking mode with at least 50-60 oscillations per minute for 5-6 hours a day at oscillation amplitude of at least 5 cm. After shuttling is completed, the obtained suspension is subject to sedimentation for removal of particles with the size of more than 3.5 mcm and less than 0.5 mcm. The standard specimen of turbidity of bacterial suspensions includes a micro suspension of chemically neutral borosilicate glass with the particle size of 0.5-3.5 mcm; with that, content of particles of this fraction is at least 95%.
EFFECT: inventions allow simplifying a method for obtaining a standard specimen of turbidity of bacterial suspensions and simultaneous improvement of stability of the standard specimen.
4 cl, 2 dwg, 2 tbl, 8 ex
SUBSTANCE: method of detecting stem cancer cells is provided, based on incubation of cell samples with fluorescence dyes and subsequent identification of cancer cells in the ultraviolet light, where during the incubation of cell samples, which is carried out with the fluorescent dye, incorporated covalently into double-stranded DNA fragments, the internalization of extracellular exogenous fragments of double-stranded DNA into the intracellular space of stem cells included in the composition of the sample cells, is provided by incubation of cell samples in the solution of the preparation of the fragmented double-stranded DNA at a ratio of 0.5-1 mcg DNA per 1000000 cells in suspension or on a section of tissue for 60-120 minutes, and identification of cancer cells as stem is carried out in ultraviolet light with a wavelength corresponding to the absorption maximum of the fluorochrome inserted into the molecules of fragments of the double-stranded DNA, and the double-stranded DNA fragments are used as DNA fragments of Alu human repeat, enzymatically labelled by precursor comprising covalently sewn fluorochrome dye, or labelled by direct chemical introduction of fluorochrome.
EFFECT: due to increasing the efficiency of detecting stem cancer cells in the initiating condition the invention can be used in medicine.
2 cl, 11 dwg
SUBSTANCE: invention proposes an inoculation loop for cultivation of microorganisms. Bacteriological loop includes a handle on one side and a detachable working element on the other side. The working element represents a metal rod with an eyehole on its end. The working element near a coupling is equipped with metal limiters located perpendicular to the working element. Metal guides are fixed on ends of metal limiters. Guides are located along the metal rod with an eyehole at the distance from it, which is equal to external radius of test tube cross-section.
EFFECT: improvement of characteristics.
3 dwg, 1 tbl
SUBSTANCE: method of indicating hospital strains of Pseudomonas aeruginosa, Staphylococcus aureus, Enterobacter cloacae involves identification of an isolated pure culture of bacteria, inoculating the identified bacteria on blood-meat infusion agar while washing accumulated bacteria - S.aureus - after 12 hours, and Exloacae and P.aeruginosa - after 8 hours with sodium chloride solution. Electrical resistance of the suspension of bacteria in the sodium chloride solution is determined at bacteria concentration of 500000 in 1 ml in a dc electric field with 2.8 V across the electrodes. If electrical resistance for P.aeruginosa bacteria ranges from 579 to 674 kom, S.aureus 545-642 kom, Exloacae 452-584 kom and epidemiological data on recording five or more disease cases from one infection source, the isolated infectious agent is considered a hospital strain.
EFFECT: invention cuts time for conducting laboratory investigations and detecting circulation of hospital strains in hospitals, use of the invention does not require intraspecific identification of bacteria.
2 tbl, 3 ex
FIELD: microbiology, in particular method for detection of transformed coryneformic bacterium variants useful in production of specific preparations.
SUBSTANCE: coryneformic bacterium cells in 0.5-0.55 % sodium chloride solution are exposed with ultrasound (40-45 kHz, 83-85 mA, 5-30 min) and after that morphological changeability and stability thereof are determined. During exposing with 5 min interval change of cell morphology is analyzed, viable cells amount is determined, and presence of morphological changeability is evaluated based on formation of L-transformed cells being exposed for 5-15 min. Cell stability is evaluated based on reduction of viable cells amount: at 5-15 min exposition viability is evaluated as high, 16-25 min - as intermediate, and at 25-30 min exposition viability is absent.
EFFECT: method for detection of coryneformic bacterium changeability of improved effectiveness.
1 dwg, 2 tbl
SUBSTANCE: nutrient medium comprises nutrient agar, dry, of Black Sea sprat, para-aminobenzoic acid, tris-(hydroxymethyl) aminomethane (tris buffer), salicin, neutral red, L-tryptophan, 5-brom-4-chloro-3-indolyl β-D-glucuronide of cyclohexylammonium salt, 2-nitrophenyl β-D-galactopyranoside and distilled water in a predetermined ratio of components.
EFFECT: invention enables to improve the differentiating properties of the nutrient medium and to reduce the time of isolation of causative agents of urological infections.
1 tbl, 3 ex
SUBSTANCE: method involves assessing radioreaction of the vaginal mucosa by visual examination, as well as assessing the vaginal epithelial condition by lactic acid bacilli testing and measuring by bacterioscopical and bacteriological analyses, performing the cytological examination of the surface and intermediate squamose epitheliocytes of the vaginal discharge followed by measuring and finding atrophic and dyskeratotic damages of the above cells. The laboratory examinations precede the radiation exposure and are repeated with the total basic dose (TBD) 20 Gy, 40 Gy and 60 Gy and 1 month after the completion of treatment.
EFFECT: invention is characterised by accessibility and low cost of production, and can be used in clinical practice for the purpose of the objective assessment of the vaginal mucosa health in radiation therapy of malignant new growths, particularly for the assessment of the early radiation reactions and injuries of the vaginal mucosa formed in the course of the therapy or in 3 near months thereafter, for the purpose of prediction of the developing radiation complications in the vagina, and if needed, the correction of the radiation exposure regimen, for the purpose of the radiation therapy compliance.
SUBSTANCE: invention relates to the field of biochemistry. Claimed is a method of evaluating cell viability in a microbioreactor by means of an optical light guide. The method includes the placement of cells into a membrane compartment of a replaceable cell unit of the microbioreactor, application of a working solution of a vital dye, introduction of the dye into the microbioreactor compartment. After the introduction incubation of the cells in the vital dye solution and removal of the vital dye solution, which has not bound with the cells, are performed. Removal is performed by the replacement of the incubation solution with a growth medium, which does not contain dye. The optical light guide, connected to a spectrometer, is brought into contact with an optically transparent material with the replaceable cell unit under the membrane compartment of the microbioreactor. After that, the support spectrum of a fluorescent signal is measured as an integral of the intensity of fluorescence on the membrane compartment of the microbioreactor, in which the cells to be analysed are absent. Also measured is the spectrum of the fluorescent signal as the integral of the intensity of fluorescence on the membrane compartment of the microbioreactor with the analysed cells. After that, the support spectrum of the fluorescent signal for the membrane compartment of the microbioreactor without the cells to be analysed is subtracted from the obtained spectrum of the fluorescent signal for the membrane compartment with the analysed cells. The quantity of the viable cells in the membrane compartment of the microbioreactor is calculated on the basis of the obtained value of the fluorescence signal intensity.
EFFECT: invention makes it possible to quickly determine viability of the cells under an impact of influencing factors in a real time mode in the microbioreactor.
6 cl, 3 dwg, 5 tbl, 5 ex
SUBSTANCE: method for estimating the bifidus bacteria and lactic acid bacilli in experimental animal's gastrointestinal tract involves preparing bacterial mutants on dense nutrient media with increasing rifampicin concentrations from 10 mcg·ml-1. The grown spontaneous mutants are selected, and the dense nutrient rifampicin medium is re-inoculated in the concentration of up to 160 mcg·ml-1. The mutants characterised by hereditarily stable rifampicin resistance are selected for administration into laboratory animals in the concentration of 150-160 mcg·ml-1 in the nutrient medium. The amount of the administered mutants is calculated. After keeping the animals, the selected faeces are suspended in isotonic sodium chloride. A supernatant is selected, and the dense nutrient medium containing rifampicin 110 mcg·ml-1 is inoculated in a Petri dish. It is incubated for 72 h in the microaerophilic environment at 37°C. The plate count is measured, and the growth-positive bacteria count is re-calculated per 1 g of faeces. A portion of the growth-positive microorganisms of the administered count is determined and used to state the survival.
EFFECT: invention enables effectively determining the portion of the positive-growth bifidus bacteria and lactic acid bacilli passed through the animal's gastrointestinal tract.
2 dwg, 3 tbl, 3 ex