Apparatus for fluorescent spectroscopy of biological tissue
SUBSTANCE: apparatus comprises a fluorescent-reflective spectrometer, which includes an illumination system and spectrometric system connected to a Y-shaped fibre-optic probe. The apparatus is further provided with two channels, one of which intended for supplying liquid to the investigated organ to wash off blood and is connected to a pump, and the other channel is intended for sucking the liquid and blood from the investigated organ and is connected to a pump. Both channels and the distal end of the fibre-optic probe are placed in a ferrule to form a fibre-optic probe. The ferrule is in the form of a metal cylinder with a socket at the end which is adjacent to the investigated organ.
EFFECT: high accuracy and consistency of measurement results, enabling investigation of the heart within the body.
7 cl, 5 dwg
The invention relates to medicine, in particular to surgery, to medical equipment, namely, devices for fluorescence spectroscopy of biological tissue and can be used for intraoperative assessment of functional and metabolic status of biological tissues, including to determine the state of the myocardium and other organs in ischemic damage.
For intraoperative assessment of functional and metabolic status of biological tissues, including to determine the state of the myocardium and other organs in ischemic damage, can be used fluorescence spectroscopy. The method is based on the phenomenon that at the early stage of ischemia, the accumulation of excessive amounts of NADH in breach of its oxidation to NAD+ through the chain of mitochondrial transport of electrons, which have the ability to glow in the visible spectrum when excited by ultraviolet rays, and the intensity of the glow depends on the location in the oxidized or reduced state. Thus, the phenomenon of autofluorescence fabrics can be used for non-invasive registration of ischemic changes in organs and tissues. Important aspects of this technology are low invasiveness and technical simplicity of the methodology of Enki the viability of the tissue.
A device for laser-induced fluorescence spectroscopy (LIFAS) to determine ischemia and hypoxia in biological tissue (US 6697657 Method and devices for laser induced fluorescence attenuation spectroscopy (LIFAS)) containing lighting and spectrometric systems, fiber-optic harness connected to the light and spectrometric systems, probe, which houses the working end of the fiber optic bundle, and a processor for processing signals, lighting and spectrometric systems. The use of the device provides high accuracy results in the experiments performed ex vivo, for example, on a stand-alone perfusion heart of the laboratory animal.
However, in similar experiments in vivo, when the heart is still in the body, carrying out such measurements difficult or impossible. This is due to the fact that the blood in the operative field, inevitably falls into the gap between the probe and the surface of the examined tissue, this leads to a decrease in the signal due to absorption by the hemoglobin of the exciting and fluorescent radiation and, as a consequence, instability results. The same difficulties arise when conducting measurements in a clinical setting.
Also known spectrometer for fluorescently-reflective biomedical research (KangUk, Papayan, C., Berezin Century B. Petrishchev N. N., Galagoudza M M Spectrometer for fluorescently-reflective biomedical research // Optical magazine. 2013. So 80. No. 1. C. 32-38), which is the closest to the claimed invention and is selected as a prototype.
Known spectrometer for fluorescently-reflective biomedical research includes led illuminator generating radiation in the near ultraviolet region with a Central wavelength of 365 nm, Υ-shaped fiber-optic probe, which unites in its distal part of the fiber illumination channel and a detection channel (measurement channel), as well as the spectrometer and the computer, which registers the spectrum in the range of 350-750 nm.
However, the known spectrometer for fluorescently-reflective biomedical research has the same drawbacks as similar, i.e. functional disabilities that do not allow for the in-vivo experiments.
The technical object of the present invention is to expand the functionality of the device for fluorescence spectroscopy of biological tissue, as well as improving the accuracy and stability of measurement results in the surgical field.
To achieve a technical result, the device for fluorescence spectroscopy of biological tissue containing fluorescence-reflective spectrometer, including lighting and spectrometric systems, each of which is connected to the appropriate output Υ-shaped fiber-optic probe made from the measurement and illumination fibers, forming respectively illuminating and receiving channels, and a computer, according to the invention is further provided with two channels, one of which is for the supply of liquid to the monitoring body for flushing blood and connected to the pump, and the other channel designed for aspiration of fluid and blood from the test body, is connected with the pump, both the channel and the distal end of the fiber-optic probe placed in the tip, forming a fiber-optic probe and the handpiece made in the form of a metal cylinder with a bell on the end adjacent to the studied organ with two fittings designed for connection of pump and pump to the respective channels.
To achieve a technical result in the device for fluorescence spectroscopy of biological tissue channel for the fluid and the channel for aspiration made in the form of two coaxial metal cylinders, the axes of which coincide with the axis of the fiber-optic probe, or a channel for the fluid and the channel for aspiration made in the form of two cylinders, the axis of which is parallel to the axis of fiber-optionscom the probe, and the distal end of the fiber optic probe is additionally placed in thin-walled metal tube.
In comparison with the known analogs of the proposed solution allows you to extend the functionality of the device by conducting experiments ex vivo, that is, on a stand-alone perfusion heart, but also in-vivo experiments, when the heart is in the organism. This is made possible by offering the essential features that not only enhance the functionality of the device, eliminating the dependence of the measurement results from the presence of blood in the operative field, but also to improve the accuracy of diagnosis of ischemic status of individual sections of the studied organs during the operation and stability of the measurement results in the surgical field.
Run a piece of metal (e.g. stainless steel) simplify and reduce the time of the sterilization process.
Thus, the present invention provides the achievement of the task.
The proposed solution is a new, not known in practice, the development of fiber-optic spectrometers, and the set of distinctive features is not necessary in the prior art. The invention is industrially applicable because of the simplicity of design of the disorder and popularity of technological processes of manufacture of individual elements of the device. This solution involves the use of modern materials and manufacturing methods, serial production industry.
The invention is illustrated by drawings, where:
in Fig. 1 shows a schematic diagram of a device for fluorescence spectroscopy of biological tissue with channels for the fluid and aspiration performed in a coaxial manner;
in Fig. 2 shows the tip in cross-section a-A;
in Fig. 3 - range of autofluorescence infarction in the state of perfusion;
in Fig. 4 - range of autofluorescence infarction in a state of ischemia;
in Fig. 5 - dependence of the integral signal autofluorescence in the range 440-460 nm from the time elapsed since the beginning of the experiment when the cyclic ischemia/reperfusion.
Device for fluorescence spectroscopy of biological tissue (Fig. 1) includes a light source 1 and the spectrometer 2, forming together a fluorescently-reflective spectrometer, a pump for supplying fluid 3, the pump suction 4, Υ-shaped fiber-optic probe, one end of which is made in the form of a harness formed of the illuminating optical fibers, and is illuminating fiber channel 5 connected to the light source 1, the other end of the fiber optic probe is formed from the measuring optical fiber and is a foster fiber to the cash 6, coupled with a spectrometer 2, and also contains a channel for the fluid on the inspected body 7 and the aspiration channel 8, which may be in the form of two coaxial metal cylinders, the axes of which coincide with the axis of the fiber optic probe or in the form of two cylinders, the axis of which is parallel to the axis of fiber-optic probe. The device also contains the tip 9, the socket 10, adjacent to the studied organ and executed as a single unit with the tip 9, the computer 11. The distal end 12 of the fiber-optic probe is made in the form of the loom with the hexagonal packing of six illuminating optical fibers, the center of which is located a measuring optical fiber, and placed in the tip 9. At the tip 9 are also channel the fluid 7 and the aspiration channel 8, which together with the distal end 12 of the fiber-optic probe, optionally placed in thin-walled metal tube to form a fiber-optic probe. The cannula 13 and 14 (Fig. 2) designed to connect a channel for the fluid 7 and the aspiration channel 8 respectively to the pump 3 and the pump 4 through the nozzles 15 and 16 (Fig. 2), which is provided with a lug 9. The connection of lighting fiber channel 5 and the receiving fibre channel 6, respectively, with the light source 1 and the spectrometer 2 is carried out using connector is, for example SMA 905. As of the luminaire 1 can be used, for example, led illuminator own elaboration on the basis of a powerful led LEDEngin LZ1-00U600. As a spectrometer 2 is used, for example, the spectrometer Avantes-2048-USB2, as the pump 3 used peristaltic pump.
Device for fluorescence spectroscopy of biological tissue works as follows.
Fiber-optic probe is superimposed on the monitoring body of the socket 10, which creates a vacuum in the area of contact with the surface of the examined body. The generated vacuum ensures a reliable contact of the distal end of the fiber-optic probe with the body, and fluid flow for flushing the blood prevents the leaking of blood into the gap between the distal end of the probe and the surface of the examined body. After installation of fiber-optic probe, the monitoring body is simultaneously supplied stimulating fluorescence radiation with a wavelength of 365 nm from a light source 1, the illumination channel 5 fiber-optic probe, and a fluid (water or saline) from the pump 3 through the cannula 13 and channel 7 for flushing blood from the surface of the examined body and is made to aspirate fluid and blood through the channel 8, the cannula 14 with a pump 4. In the tissue of the organ to be investigated is excited fluorescence of various endogenous f is uorophores, including NADH. Fluorescent radiation fabric is perceived by the receiver channel 6, which passes it to the input of the spectrometer 2, which is analog-to-digital conversion received by the receiving channel 6 signal fluorescence excitation, primary processing and transmission to the computer 11 via the USB2 interface.
The use of a device for fluorescence spectroscopy of biological tissue can be illustrated by the example of one of the experiments studies of various animal organs, particularly the heart of the rats spent in the fgbi "Almazov center them. C. A. Almazov" of the Ministry of health of Russia. The experiment was to study the dynamics autofluorescence heart during execution of repeated episodes of brief ischemia and reperfusion, known as preconditioning (adaptive phenomenon, which consists in increasing the resistance of the myocardium to the subsequent duration of ischemia). Experience in-vivo were carried out on isolated perfusion on Langendorff heart rats using the layout of the present invention in the mode of continuous registration of an integrated fluorescence intensity at the maximum emission of NADH at a wavelength of 450±10 nm at excitation 365 nm. Fiber-optic probe at the Desk were in constant contact with a working heart. The result is Alicia in fiber-optic probe channel for the fluid and aspiration channel, continued destruction of blood at the site of contact of the probe with the surface of myocard that provided the measurements are independent from the presence of blood in the operative field. Thus it was possible to register the fluorescence spectra of the myocardium in the state of perfusion (Fig. 3) and ischemia (Fig. 4), which shows an increase in signal intensity of autofluorescence in the field of 440-460 nm ischemia. In addition, failed to register the dependence of the integral signal autofluorescence in the range 440-460 nm from the time elapsed from the beginning of the experiment (Fig. 5). The experiment was conducted three cycles of ischemia 17, after which followed a period of reperfusion 18 (Fig. 5). The experiment lasted for 11 minutes. Ischemia was induced in a time period 2:45-3:45, 5:45-6:45 and 8:45-9:45. In Fig. 5 there is a clear correspondence between periods of ischemia and reperfusion and the level of the signal from the spectrometer.
Thus, the use of the claimed invention allows to extend the functionality of a device for fluorescence spectroscopy of biological tissue, as well as to improve the accuracy and stability of measurement results in the surgical field.
1. Device for fluorescence spectroscopy of biological tissue containing fluorescently-reflective spectrometer, including lighting and spectrometric system, each of Kotor is x connected to the appropriate end of the branched part of the Υ-shaped fiber-optic probe, made from the measurement and illumination fibers, forming respectively illuminating and receiving channels, and a computer, characterized in that it is further provided with two channels, one of which is for the supply of liquid to the monitoring body for flushing blood and connected to the pump, and the other channel designed for aspiration of fluid and blood from the test body, is connected with the pump, both the channel and the distal end of the fiber-optic probe placed in the tip, forming a fiber-optic probe and the handpiece made in the form of a metal cylinder with a bell on the end, adjacent to the studied organ with two fittings designed for connection of pump and pump to the respective channels.
2. Device for fluorescence spectroscopy of biological tissue under item 1, characterized in that the funnel with a tip made as a single unit.
3. Device for fluorescence spectroscopy of biological tissue under item 1, characterized in that the channel for the fluid and the channel for aspiration made in the form of two coaxial metal cylinders, the axes of which coincide with the axis of the fiber optic probe.
4. Device for fluorescence spectroscopy of biological tissue under item 1, characterized in that the channel for the fluid and the channel for ASPI is then made in the form of two cylinders, axis which is parallel to the axis of fiber-optic probe.
5. Device for fluorescence spectroscopy of biological tissue under item 1, characterized in that the distal end of the Υ-shaped fiber-optic probe, made in the form of the loom with the hexagonal packing of six illuminating optical fibers, the center of which is located a measuring optical fiber.
6. Device for fluorescence spectroscopy of biological tissue under item 1, characterized in that the ends of the branched part of the Υ-shaped fiber-optic probe connected respectively with lighting and spectrometric systems through connectors.
7. Device for fluorescence spectroscopy of biological tissue under item 1, characterized in that the distal end of the fiber optic probe is additionally placed in thin-walled metal tube.
SUBSTANCE: invention relates to biotechnology and represents method of spectral analysis of fluorescent properties of DNA nucleotide successions. Claimed invention can be applied for genetic diagnostics, research of mitogenetic irradiation of cells, study of coding hereditary and proliferative information. Method includes preparation of water solutions of dyes of basic fluorescence colours. Background fluorescence of dye is measured in respective detection channels by means of fluorescent detector FDG-001. DNA sample is added to dye solution in quantity 150-200 ng. Fluorescence of dye solutions is measured. Results of analysis are registered in form of presentation of values in conventional units of positive or negative increase of fluorescence of dyes relative to their initial background before addition of DNA sample in form of building columnar diagrams or curves of signal growth dynamics in time. Results of dye fluorescence growth are interpreted.
EFFECT: claimed invention makes it possible to determine nucleotide rebuildings of telomeric DNA ends, point mutations, polymorphisms of gens, chromosome rebuildings, and change of karyotype or cell genome.
15 cl, 16 dwg, 1 tbl, 6 ex
SUBSTANCE: invention relates to the field of molecular biology and biochemistry. A device consists of a light source, the radiation of which is directed on a transparent substrate with oligonucleotides immobilised on its surface and a system of detecting the intensity of light, which passed through the substrate, located under it. The substrate contains at least two zones, with a layer of oligonucleotides, non-specific to the nucleotide sequence under study, being immobilised on the surface of one of them, and a layer of nucleotides, specific to the nucleotide sequence under study, being immobilised on the surface of the other zone. The detection system contains at least two photosensitive independent sections, each of which is illuminated by the radiation, which passed through only one zone.
EFFECT: device makes it possible to carry out the qualitative and quantitative analysis of the nucleotide sequences, increases the accuracy of the identification of the nucleotide sequences.
3 cl, 5 ex
FIELD: measurement equipment.
SUBSTANCE: invention relates to a measuring method of variations of fluorescence intensity of voltage-sensitive fluorochrome depending on a potential gradient or ionic strength, which involves addition to the voltage-sensitive fluorochrome of an ionising compound to induce variation of potential or ionic strength, as well as addition of vitamin E and/or cholesterol to increase variation of potential or ionic strength as to voltage-sensitive fluorochrome. Besides, the invention relates to a potential measuring method of actions of cultivated cardiomyocytes.
EFFECT: invention provides measurement of fluorescence intensity of voltage-sensitive fluorochromes or voltage-dependent quantitative variations of their fluorescence intensity without using any such materials (membrane carriers), as cells or two-layer lipidic liposomes.
10 cl, 3 ex, 5 dwg
SUBSTANCE: invention can be used when determining content of benzene, toluene and xylene (BTX) vapour in urban air, air in residential facilities, chemical laboratories, filling stations and oil processing enterprises, in gas emissions of industrial plants. The method of determining concentration of benzene, toluene and xylene vapour in a gaseous mixture comprises placing material containing boron difluoride dibenzoylmethane (BF2DBM) fluorophore or a methyl- or methoxy derivative thereof into the gas mixture, illuminating the material with light in the wavelength range of 355-400 nm and measuring fluorescence intensity of the material in the wavelength range of 400-550 nm. Unlike the existing method, measurement is carried out on not less than two spectral channels, wherein the number of channels is selected not less than the number of determined components in the mixture plus one. The measured values are then used to calculate relative intensity of spectra of the fluorophore and exciplexes thereof with benzene, toluene and xylene. Concentration of benzene, toluene and xylene is then determined from the ratio of intensities of the corresponding exciplex to the intensity of BF2DBM.
EFFECT: simultaneous continuous selective measurement of benzene, toluene and xylene in gaseous mixtures in a wide range of concentrations with short reaction time.
2 cl, 4 dwg, 1 tbl
SUBSTANCE: invention relates to the field of biochemistry. Claimed is a method of evaluating cell viability in a microbioreactor by means of an optical light guide. The method includes the placement of cells into a membrane compartment of a replaceable cell unit of the microbioreactor, application of a working solution of a vital dye, introduction of the dye into the microbioreactor compartment. After the introduction incubation of the cells in the vital dye solution and removal of the vital dye solution, which has not bound with the cells, are performed. Removal is performed by the replacement of the incubation solution with a growth medium, which does not contain dye. The optical light guide, connected to a spectrometer, is brought into contact with an optically transparent material with the replaceable cell unit under the membrane compartment of the microbioreactor. After that, the support spectrum of a fluorescent signal is measured as an integral of the intensity of fluorescence on the membrane compartment of the microbioreactor, in which the cells to be analysed are absent. Also measured is the spectrum of the fluorescent signal as the integral of the intensity of fluorescence on the membrane compartment of the microbioreactor with the analysed cells. After that, the support spectrum of the fluorescent signal for the membrane compartment of the microbioreactor without the cells to be analysed is subtracted from the obtained spectrum of the fluorescent signal for the membrane compartment with the analysed cells. The quantity of the viable cells in the membrane compartment of the microbioreactor is calculated on the basis of the obtained value of the fluorescence signal intensity.
EFFECT: invention makes it possible to quickly determine viability of the cells under an impact of influencing factors in a real time mode in the microbioreactor.
6 cl, 3 dwg, 5 tbl, 5 ex
SUBSTANCE: invention relates to application of bis(2,4,7,8,9-pentamethyldipyrrolylmethen-3-yl)methane dihydrobromide as fluorescent zinc (ii) cation sensor.
EFFECT: invention will make it possible to increase fluorescent activity of heterocyclic organic compound with respect to zinc (II) ion in presence of other ions of metals.
1 tbl, 40 ex
FIELD: machine building.
SUBSTANCE: invention refers to the field of DNA sequencing, in particular, to the DNA sequencing with the use of time-controlled fluorescence determination for identification of DNA bases The device comprises a field of embedding for containment of the sequencing reaction components, light sources featuring a capability of emitting the light pulse of a definite wave length, detector pixel, detector, output featuring a capability of transmitting an electric signal from the detector pixel, gating means for detector gating, at that, the detector pixel comprises additionally the first and the second storage batteries The first storage battery is provided with a capability of accumulating electric signal from the detector in response to the first light pulse, while the second storage battery is provided with a capability of accumulating electric signal from the detector in response to the second light pulse.
EFFECT: increased rate of receiving sequencing results.
12 cl, 7 dwg
SUBSTANCE: sensor includes semiconductor nanocrystals (quantum dots), imbedded into a near-wall layer of track pores of polyethylene terephthalate membranes, with the pores remaining empty. If ammonia vapours are present in an air sample, ammonia molecules bind with quantum dot surfaces, which results in decrease of quantum dot luminescence.
EFFECT: invention solves the tasks of increasing sensitivity, accuracy of determination of ammonia vapour concentration, terms of exploitation and simplification of the sensor manufacturing.
5 dwg, 1 ex
SUBSTANCE: mix of test gases is forced through test cell. Fluorescent radiation is excited therein by readjustable solid-state lasers with wavelengths corresponding to lines with maximum absorption by isotopes 129I and 127I and nitrogen dioxide. Concentration of said isotopes 129I and 127I and nitrogen dioxide are defined in analysed mix by formulae that allow for the composition of buffer gases.
EFFECT: higher sensitivity of determination.
2 cl, 2 dwg
SUBSTANCE: method comprises placing the fluorescent test-objects in the control and analyzed samples, irradiation with the excitation light, definition of fluorescence characteristics, by which change the toxicity of the controlled environment is assessed. Microalgae of species Scenedesmus apiculatus are used as test-objects, which are previously isolated from environmentally safe areas of the test water reservoirs.
EFFECT: use of the claimed method enables to assess quickly and accurately the toxicity of water and bottom sediments of the Azov and Black Seas.
6 tbl, 4 ex
SUBSTANCE: multispectral camera comprises a diaphragm, a dispersing element, a lens, a microlens array, a photodetector and a processor. Radiation enters the multispectral camera through the diaphragm, which has at least one opening, and is directed by the dispersing element in different, wavelength-dependent directions. The lens focuses the radiation from the dispersing element on an image plane. The microlens array receives radiation from the lens and directs the radiation to the photodetector. The processor forms a multispectral image based on values of signals from the photosensitive elements of the photodetector.
EFFECT: forming multispectral images without using scanning systems and removable filters, improved time resolution and simple design of the device.
15 cl, 7 dwg
SUBSTANCE: apparatus comprises: an illumination device which can generate a light beam in one wavelength range, a probe (PRB) which is such that the light beam from the illumination device interacts with the analysed fluid medium (20), and a spectrum analysis device which can receive the light beam after interaction thereof with the analysed fluid medium and output luminous intensity measurements for different wavelength ranges. The probe (PRB) has a retroreflector-type reflector (13) which reflects in the direction of the light source, which is placed such that it receives the light beam passing from the illumination device through the analysed fluid medium, and reflect it essentially in the opposite direction, while easily expanding it, to a receiving light-guide (12) which is connected to the reflector and the spectrum analysis device so as to receive the light beam passing through the analysed fluid medium and transmit it to the spectrum analysis device.
EFFECT: enabling compensation for alignment defects between optical elements of the device.
27 cl, 12 dwg
SUBSTANCE: spectrometer has an infrared source, a gas sample cell, a scanning mirror supporting a diffraction grating, having a plurality of parallel lines and lying on the path of the infrared beam after passage thereof through the gas sample cell such that the diffraction grating splits the infrared beam into a first-order beam and a second-order beam, a resonance scanner excitation system and a divider which can direct first-order and second-order beams on the first and second paths, respectively. There is a focusing element, a detector and a detector reading circuit on each of the two paths.
EFFECT: high reliability and compactness.
13 cl, 22 dwg
SUBSTANCE: measuring device (14) has a sensor (16) for determining at least one component and/or at least one property of the material (4). The sensor (16) has at least one light source (18) which directs at least one light beam (20) onto the investigated material (4). The measuring device (14) has at least one reference object (34, 32, 33) for calibrating the measuring device (14). Part of the light beam (20) of the light source (18) is diverted onto the reference object (34, 32, 33).
EFFECT: avoiding the need for alternate switching from the investigated material to the reference object.
12 cl, 4 dwg
SUBSTANCE: invention refers to medicine and concerns a device for the infrared exposure on the human skin. The device is presented in the form of a magnetic resonance tomographic scanner and comprises a receive/transmit channel, a three-dimensional localisation unit, a microprocessor controller and a display. The device is also provided with a local exposure unit presented in the form of a manipulator with an IR laser, a lens and a marker for laser beam binding to a coordinate system of the analysed area.
EFFECT: higher accuracy and intensity of the local exposure, as well as providing object tissue change tracking.
3 cl, 1 dwg
SUBSTANCE: group of inventions refers to medicine. A device comprises a sapphire probe with longitudinal canals accommodating optical fibres; some of them are applicable for supplying a radiation exciting fluorescence and coagulating the radiation into tissue destruction from attached radiation sources; the others are applicable for supplying the fluorescence radiation onto a radiation detector. The sapphire probe also has an open aspiration canal connected to an aspirator by means of a hose. The relative position of the canals enables detecting fluorescence from an area matched with the tissue coagulation and aspiration area. A flat end of a point may be presented at a right angle to a probe axis and/or may have inclined refractive edges. The other open canal comprises metal contacts attached to an electrocoagulator. Implementing the method requires measuring fluorescence in a small neighbourhood of the sapphire probe. If certain threshold fluorescence has been exceeded, a laser coagulation and/or electric coagulation and aspiration of the coagulated and tumour tissue through the same sapphire probe is conducted.
EFFECT: group of inventions enables increasing an accuracy of tumour delimitation with using a fluorescent diagnostics, reducing a time required for the complete tumour removal and enables removing the difficult tumours.
8 cl, 8 dwg, 1 ex
SUBSTANCE: invention relates to medical equipment, namely to devices of medical and photobiological purpose, intended for realisation of process of luminescent diagnostics of cancer on the basis of application of a number of rare-earth metal complexes of porphirines and is aimed at increasing sensitivity of measurements of luminescence intensity within the spectrum range 900-1100 nm, which leads to sharp reduction of dose of preparation, introduced into patient's organism and eliminates presence of any kind of toxicity during procedure realisation. Device contains source of laser irradiation, fibre-optic probe, including light guide for delivery of laser irradiation to biotissue, whose inlet end is connected with laser irradiation source via input device, and receiving fibre-optic system for luminescence detection, unit of registration and processing of luminescent signal, first system of lenses, located at the said unit input, pulse generator, second system of lenses, located at the output of receiving fibre-optic system of probe, and reflective interferential filters, located between said systems of lenses.
EFFECT: increased sensitivity of measurements.
7 cl, 2 dwg
SUBSTANCE: invention is referred to the area of medical technology, namely to X-ray machines, and can be used for visual control of the area on patient body irradiated by an X-ray machine. The device consists of the frame fixed on the X-ray emitter with two couples of moving flat X-ray-nontransparent screens located perpendicularly to each other forming central square window for passage of diverging X-ray beam, the peak of which matches the focus of X-ray emitter, as well as optic system forming the light centering of incident X-ray radiation on the object. The optic system consists of four linear lighters each of which is flexibly fixed on its X-ray-nontransparent screen and is outfitted with the turning mechanism.
EFFECT: increased brightness of light field lighting on the object; excluded the need for periodical alignments of light and radiation field borders.
5 cl, 4 dwg