Biologically active food additive

FIELD: food industry.

SUBSTANCE: biologically active food additive strengthening the organism adaptive power and body defences and having anti-inflammatory and antioxidant activity contains vegetal origin components represented by a complex extract of devil's-club root, Rhaponticum carthamoides root, Hedysarum neglectum Ledeb root, celery roots and leaves, rhodiola rosea root, Japanese angelica tree roots, boschniakia rossica roots, Hungarian sainfoin herb, magnolia-vine fruits; additionally the additive contains chitosan, trepang fermentative hydrolysate, ascorbic acid, taurine, glutathione, nicotinamide, vitamin B1, vitamin B2, vitamin B6, vitamin B12, folic acid, anhydrous calcium chloride, magnesium chloride, zinc chloride, bee honey at preset ingredients ratio.

EFFECT: biologically active food additive promotes effective strengthening of the organism adaptive power and body defences and human aging retardation.

4 tbl, 6 ex

 

The invention relates to food industry, namely, biologically active additives to food - parapharmaceutical containing substances of marine and vegetable origin, reinforcing adaptive and protective functions of the organism.

Known treatment-and-prophylactic food composition "Herbamare, including sweetener, soluble dietary fiber, extracts hips, and/or the herb St. John's wort, and/or lespedeza, and/or licorice root, and/or protein-containing raw material, and/or medicinal plants or their mixtures, and/or biologically active substances of marine life, and intended for food supplements as a restorative remedy for various diseases, including those suffering from obesity [EN 2093046 C1, publ. 06.10.1997].

Known biologically active food Supplement containing soluble dietary fiber, biologically active substances of marine and vegetable origin, color, citric and ascorbic acid, taurine, potassium sorbate, sugar, water, as a source of biologically active substances of plant origin contains Astragalus postively or sherstystovustyi, Arnica mountain, bergenia, hawthorn blood-red, birch, Valerian medicinal, dried grapes, hibiscus, St. John's wort, strawberry Le is s, viburnum ordinary, meadowsweet, lespedeza kopeechnikova and others, and as a source of biologically active substances (BAS) of marine origin - scallop hydrolyzate food, mussels hydrolyzate food, salmon ROE fish hydrolyzate food, squid hydrolysate food, crab hydrolyzate food, sea urchins flat frozen, eggs of sea urchins cheap ovary caviar, as soluble fiber Zosterin. SUPPLEMENTS improves heart function, promotes vasodilation, and strengthens capillaries and arteries and normalizes blood pressure [EN 2165719 C1, publ. 27.04.2001].

Well-known dietary Supplement containing components of plant origin and chitosan. At the same time as components of plant origin using complex extract from the roots of aralia Manchu, rose fruit, leaves and roots of Eleutherococcus senticosus and black chokeberry fruits. The components of the SUPPLEMENTS used in the following ratio: a comprehensive extract of the roots of aralia Manchu, rose fruit, leaves and roots of Eleutherococcus senticosus and the fruit of the black chokeberry - 165,0-195,0 kg; chitosan - 85,0-95,0 kg Invention allows to obtain BAD, providing restorative and enhanced immunostimulatory effects. BAA also improves the cardiovascular system, enriches the body with oxygen is m and relieves nervous and physical fatigue [EN 2333677 C2, publ. 20.09.2008].

This solution is the closest to the proposed solution.

However, the range of pharmacological actions known BAD rendered on various organs and systems of the body, not wide enough.

The technical task of the present invention is the expansion of the range of dietary supplements by creating a new biologically active additives aimed at strengthening adaptive and protective functions of the organism.

Problem solved biologically active additive to food, strengthening adaptive and protective forces of the body, has anti-inflammatory, antioxidant activity, containing components of plant origin and chitosan, which, according to the invention, as components of plant origin contains a comprehensive extract of the roots zamanihi high, the roots of leuzea root red root, roots and leaves of celery, root Golden root, the roots of aralia high, the roots of bosnjaci Russian, grass sainfoin sandy, schisandra fruit and additionally contains the enzymatic hydrolysate of trepang, ascorbic acid, taurine, glutathione, nicotinamide, vitamin B1, vitamin B2, vitamin B6, vitamin B12, folic acid, calcium chloride anhydrous, magnesium chloride, zinc chloride, honey bee when it is followed by the eating the ratio of ingredients, in kg per 100 gave:

Chitosan10,0-15,0
Ascorbic acid30,0-35,0
Taurine0,1-0,15
Glutathioneof 0.15-0.2
Nicotinamideof 0.15-0.2
Vitamin B1of 0.15-0.2
Vitamin B2of 0.15-0.2
Vitamin B6of 0.15-0.2
Vitamin B120,04-0,06
Folic acid0,05-0,06
Calcium chloride anhydrous3,6-4,0
Magnesium chloride1,0-1,4
Zinc chloride0,65-0,7
Honey bee700,0-750,0

and l:

A comprehensive extract of the roots zamanihi high
the roots of leuzea root red root,
roots and leaves of celery, root Golden root,
the roots of aralia high, the roots of bosnjaci Russian
grass sainfoin sandy, schisandra fruit6,0-10,0
Enzymatic hydrolysate of trepang134,0-136,0

to obtain a comprehensive extract using ethyl alcohol to 39.5-40.5 percent and complex extract contains components in the following ratio in kg per 100 gave extract:

The roots zamanihi high12,0-14,0
The roots of leuzea12,0-14,0
Root red root12,0-14,0
The roots and leaves of celery12,0-14,0
The roots of Golden root12,0-14,0
The roots of aralia high12,0-14,0
The roots of bosnjaci Russ is Oh 12,0-14,0
Grass sainfoin sandy12,0-14,0
The fruits of schisandra12,0-14,0
Ethyl alcohol330,0-340,0
Distilled waterRest

The technical result - the creation of a new BUD, with a broader range of pharmacological activity compared with the known BAD. The components of the proposed BAA claimed in qualitative and quantitative ratio exhibit synergistic properties, providing for its tonic, hepatoprotective, antioxidant and anti-inflammatory effects on various organs and systems of human body activity.

Complex extracts of plants, part of the claimed SUPPLEMENTS, exhibits enhanced adaptogenic effect, raising nonspecific resistance of the body through the nervous, immune and endocrine systems. Components included in the composition of the extract, are chosen in the ratio, effectively mobilizing the body's defenses.

Devil's high (Oplopanax elata) contains the roots of 5% essential oil, triterpene glycosides, alkaloids, phenolic compounds, minerals, coumarins, flew the formation, cracks saponins. Drugs zamanihi stimulate the Central nervous system, used for the treatment of depression with fatigue, have blood-forming properties and other

The roots of aralia high (Aralia elata) contain triterpene saponins, flavonoids, alkaloids, carbohydrates, essential oil, organic acids. Drugs aralia high have tonic, cardiotonic, antioxidant, anti-stress, gonadotropic, hypoglycemic action. In medicine preparations of this plant is prescribed for asthenic and depressive disorders, schizophrenia, hypertensive disease, impotence, diabetes, initial stages of atherosclerosis, functional disorders of the nervous system, rehabilitation after serious infectious diseases.

The roots and rhizomes Golden root (Rhodiola rosea) contain antraglikozida, essential oil, organic acids, sugars, proteins, fats, phenolic compounds, glycosides, salidroside 1%, flavonoids, trace elements (copper, manganese, chromium, silver, and others). Alcohol extract of Rhodiola used as a means of stimulating the Central nervous system, Pichinde and neurasthenic conditions, fatigue, low productivity, vegetative-vascular dystonia, psychiatric, functional diseases of the nervous system, in the region of the rehabilitation period after somatic and infectious diseases, as well as healthy people at low health. In experiments it was shown that salidroside is the protection of human erythrocytes from oxidative stress and may be a good adaptogen to increase the body's resistance to stress and fatigue.

In fruits of schisandra (Schisandra chinensis) contains sugar, tanning and coloring compounds, fatty and organic acids. In addition, fruits are found essential oil, sesquiterpenes, ascorbic acid, vitamin E, and schizandrin and schizandrol. Preparations of Chinese Magnolia vine shown in asthenic syndrome, vegetative-vascular dystonia of hypotonic type, in the period of convalescence after somatic and infectious diseases. Lemongrass is also prescribed for fatigue, decreased performance, and when doing activities, coupled with great mental and physical stress. In the complex therapy is applied when violations of sexual function in the background of neurasthenia.

Celery odorous (Apium graveolens) contains alpha-pinene, apigenin, beta-carotene, caffeic acid, coumarin, eugenol, Frolovo acid, isoquercitrin, luteolin, choline, fatty acids, rutin, amino acids, boron, calcium, macronutrients, vitamins. Is an aphrodisiac. Root celery has abducible is relevant and diuretic, and it is widely used in diseases of the kidneys and bladder.

Boshnjaku Russian (Boschniakia rossica) contains p-sitosterol, p-coumaric, oleanolic, 3-epideimology acid, methyl-p-chomarat, iridoid, alkaloids, monoterpene. In traditional Chinese medicine dried boshnjaku used as artisitically, tonic, cardiotonic and tonic to treat impotence, spermatorrhea, infertility, as well as to stimulate hormone secretion, in addition, for dizziness, nervousness, arthritis, renal failure, dry cough.

The roots of leuzea (Rhaponticum carthamoides) contain resinous substances, tannins, vitamin C, vitamin a, inulin, minerals, catechin, hesperidin. Used in medicine as a General tonic and adaptogenic drugs. Drugs of Rhaponticum shown in combination therapy with fatigue, physical and mental fatigue, reduced potency.

Sainfoin sandy (Onobrychis arenaria) contains carotene, ascorbic acid, up to 8% of carbohydrates in the seeds 7-8% of fatty oil. Preparations from this plant Improves the properties of the blood (reduce viscosity, normalizes cholesterol, sugar, hemoglobin), increase the level of male hormones (testosterone, androgens), improve sexual function.

Red root (Hdyzarum theinum) contains 30 to 40% polysaccharides, when hydrolysis which hatshepsuts galactose, xylose, galacturonic acid and rhamnose. Red root has anti-inflammatory, antioxidant, antispasmodic, vasodilator, tonic effect, normalizes the urinary system inflammation of the prostate gland and the urethra. Drugs, containing red root, contribute to the improvement of the body as a whole: normalize the activity of the gastrointestinal tract, strengthen the cardiovascular and respiratory system, improve emotional status, effectively relieve fatigue, enhance physical endurance.

Ascorbic acid is involved in the regulation of the oxidation-reduction processes, carbohydrate metabolism, clotting of the blood, regeneration of the tissues; increases the body's resistance to infections, reduces vascular permeability, reduces the need for vitamins B1B2, A, E, folic acid, Pantothenic acid. Has antiplatelet and antioxidant properties.

The amino acid taurine is a key compound in the biosynthesis of all other amino acids, is also a key component of bile, necessary for hydrolysis of fats, the absorption of fat-soluble vitamins and controlling the level of cholesterol in blood plasma. Taurine life is i.i.d. important for the absorption of sodium, potassium, calcium and magnesium protects against loss of potassium cardiac muscle, which helps to prevent potentially dangerous cardiac arrhythmia. In tandem with zinc protects brain neurons against metabolic disorders, and improves vision.

Glutathione is composed of the amino acids cysteine, glutamic acid and glycine, is a powerful antioxidant that is designed to neutralize toxic compounds, protection from destruction of blood cells, removal of oxidized lipids, recovery of ascorbic acid. The lack of glutathione leads to dysfunction of the nervous system, causing symptoms such as loss of coordination, weakness of thought processes, tremor and loss of balance. It is believed that these symptoms are associated with the development of lesions in the brain. The lack of in the body of glutathione accelerates aging.

B vitamins support the nervous system, skin, eyes, liver, oral cavity, gastrointestinal tract, musculoskeletal system and brain. The complex is involved in enzymatic reactions as coenzymes, as well as in energy production.

Calcium, magnesium, and zinc are nutrients that are vital to the functioning of the body, take part in biochemical reactions and metabolic processes of the body are cofactors of enzymes, chrome is in calcium is indispensable for the formation of bones, teeth and maintain healthy gums, protects bones and teeth from the adsorption of lead in bone.

Far East sea cucumbers (Stichopus japonicus). Modern scientific research has found that bioactive substances trepang has a rejuvenating effect contained in the tissues of trepang mucopolysaccharides and chondroitin help with inflammatory diseases of the skeletal muscles and musculoskeletal system. Triterpene and steroid glycosides contained in the trepang, possess strong antifungal and antitumor activity. Lipids trepang have broad anti-sclerotic effect, reduce excess cholesterol in the blood. Fabric trepang contain almost the entire set of water-soluble vitamins.

Enzymatic hydrolysate of trepang contains a complete set of amino acids, monosaccharides and glucosamine. In the proposed formulation the BAD hydrolysate trepang supplements chitosan in maintaining homeostasis of metabolic processes in the body.

Chitosan is a natural polymer obtained from the shells of crustaceans (shrimp and crabs), has a number of unique properties, which put him first in a series of chelators. Chitosan exhibits adjuvant, mitogenic, anti-infectious, antiviral, protivorechu the initial effect, activates the proliferation of natural killer cells that destroy tumor cells. Chitosan restores the atherogenic index to regulations, has the ability to restore the cells of organs and tissues, and activate macrophages to produce nitric oxide, which is the main neurotransmitter in the body.

Chitosan is a cationic polymer, broken down in the body by lysozyme, chitinases and chitosanase to oligomers of varying degrees of polymerization. Chitosan and its oligomers binding to anionic physiologically active compounds presented in the proposed formulation, a transport function for delivery of substances into cells. In the interaction of chitosan with physiologically active compounds of medicinal plants, hydrolyzed trepang, vitamins, macro - and microelements, peptides, amino acids observed prolonged protective effect of the claimed SUPPLEMENTS in various organs and tissues of the body.

The use of honey as an effective medicinal product is based on many of his properties, including antibacterial, bactericidal, anti-inflammatory and antiallergic action. therapeutic effect of honey contribute to the composition of sugars, minerals, trace elements, vitamins, enzymes, biologically active substances. The honey used in the t as a tonic, toning, regenerating power tool. It is used for diseases of the cardiovascular system, kidneys, liver, biliary tract, gastrointestinal tract. When used in food, honey is easily digested. In addition, honey contains a large number of aromatic substances that improve the taste of the product.

The proposed biologically active food Supplement obtained as follows.

Preparation of the starting components for the recipe.

Preparation of a complex plant extract. Prepare extracts of each plant ingredient separately. As extractant using 40% ethanol at a ratio of raw material: solvent 1:10. Extracts incubated for 10-12 days at room temperature, then filtered and unite.

Preparation of enzymatic hydrolysate of trepang. Freshly caught sea cucumbers frozen. To one volume of frozen trepang add one volume of 95% ethanol, incubated day at room temperature, wring out excess liquid, dried at room temperature and the dry residue is used for enzymatic hydrolysis.

In a glass bowl make a dry residue of sea cucumbers, water, sodium acetate, 70% acetic acid, distilled water to the final volume. Then make cal the Nations chloride anhydrous, collagenase from hepatopancreas crab. Enzymatic incubation trepang spend days at a temperature of 38-43°C. the Enzymatic hydrolysate of trepang is filtered, then the filtrate make sorbic acid and stored at 2-4°C. the composition of the hydrolysate was determined on the amino acid analyzer Biochrom-30 (Cambridge), monosaccharide is detected by A detector Ch 1/570 nM. In the enzymatic hydrolysate of trepang revealed a complete set of amino acids, and monosaccharides presents mannose, fucose, galactose and glucosamine.

Table 1 presents the results of the analysis of the hydrolyzate of sea cucumbers on the content of free amino acids.

Other ingredients of the formulation are commercial products.

Calcium, magnesium and zinc are included in the form of organic salts.

Method of preparation of the claimed BAD.

The estimated amount of the chitosan suspended in distilled water and poured the estimated amount of ascorbic acid, previously dissolved in distilled water. The mixture is then stirred by the stirrer motor to dissolve the chitosan.

In a separate glass bowl make a calculated quantity of enzymatic hydrolisate trepang, taurine, glutathione, nicotinamide, vitamin B1, B2, B6, B12, folic acid, chloride is th anhydrous calcium, magnesium chloride, zinc chloride, complex plant extract and then add distilled water to the final volume. The mixture is stirred with a motor with a stirrer.

The resulting solutions are combined poured estimated number of honey bees and stirred until a homogeneous state.

Prepare product rich yellow color, sweet-sour taste with a honey flavor, slightly astringent in the mouth. The product stored at a temperature of 2-4°C.

Enablement of the claimed invention is illustrated by examples.

Example 1. For the preparation of biologically active additives to food consumption ingredients 100 gave, kg:

td align="right"> 0,04
Chitosan10,0
Ascorbic acid30,0
Taurine0,1
Glutathione0,15
Nicotinamide0,15
Vitamin B10,15
Vitamin B20,15
Vitamin B60,15
Vitamin B12
Folic acid0,05
Calcium chloride anhydrous3,6-4,0
Magnesium chloride1,0-1,4
Zinc chloride0,65-0,7
Honey bee700,0

and l:

A comprehensive extract of the roots zamanihi high
the roots of leuzea root red root,
roots and leaves of celery, root Golden root,
the roots of aralia high, the roots of bosnjaci Russian
grass sainfoin sandy, schisandra fruit6,0
Enzymatic hydrolysate of trepang134,0

to obtain a comprehensive extract using ethyl alcohol to 39.5-40.5 percent and complex extract contains components in the following ratio in kg per 100 gave extract:

The roots zamanihi highto 12.0
The roots of leuzeato 12.0
Root red rootto 12.0
The roots and leaves of celeryto 12.0
The roots of Golden rootto 12.0
The roots of aralia highto 12.0
The roots of bosnjaci Russianto 12.0
Grass sainfoin sandyto 12.0
The fruits of schisandrato 12.0
Ethyl alcohol330,0
Distilled waterrest

Example 2. For the preparation of biologically active additives consumption ingredients 100 gave, kg:

Chitosan15,0
Ascorbic acid35,0
Taurine0,15
Glutathione0,2
Nicotinamide0,2
Vitamin B10,2
Vitamin B20,2
Vitamin B60,2
Vitamin B120,05
Folic acid0,06
Calcium chloride anhydrous3,6-4,0
Magnesium chloride1,0-1,4
Zinc chloride0,65-0,7
Honey bee750,0

and l:

A comprehensive extract of the roots zamanihi high
the roots of leuzea root red root,
roots and leaves of celery, root Golden root,
the roots of aralia high, the roots of bosnjaci Russian
grass sainfoin sandy, schisandra fruit10,0
Enzymatic hydrolysate of trepang136,0

to obtain a comprehensive extract using ethyl alcohol to 39.5-40.5 percent and complex extract contains components in the following ratio in kg per 100 gave extract:

The roots zamanihi high14,0
The roots of leuzea14,0
Root red root14,0
The roots and leaves of celery14,0
The roots of Golden root14,0
The roots of aralia high14,0
The roots of bosnjaci Russian14,0
Grass sainfoin sandy14,0
The fruits of schisandra14,0
Ethyl alcohol340,0
Distilled waterConducted Toxicological and pharmacological studies claimed BAD.

Example 3. Toxicological studies.

Preliminary Toxicological studies on mice-males line DM-1 and IAS (weighing 22±2 g) showed that the claimed BAD has a very low acute toxicity (LD50>1 g/kg) and therefore, its use is safe in a wide range of concentrations.

The study of the pharmacological activity conducted on mice-males line DM-1 and IAS (weighing 22±2 g). The experiments were performed on animals that were obtained from the nursery of laboratory animals "Pushchino" and divorced in a certified vivarium, tibah Feb RAS. Animals were kept in accordance with the international rules, approved by order No. 267 healthcare of the Russian Federation dated 19.06.2003, and "Guidance on experimental (preclinical) study of new pharmacological substances" (2005).

Example 4. Tonic effect of the claimed BAD.

As experimental animals used mice CD-1, male (18±2 g). As a positive control used drug antler deer "Pantocrine, which are produced in the form of an aqueous-alcoholic extract. It has a tonic effect upon the fatigue and strain of the organism.

Animals were divided into groups of 5 animals each: 1-I groups the - control animals that had not received drugs; 2 and 3-group I - group positive control, which had been widely known tonic preparation "Pantocrine"; 4 and 5-I - experimental group of animals that received the claimed BAD. The drugs were administered orally once a day consecutively for 5 days in a volume of 200 µl per mouse.

The final destination of the drugs was performed for 1 hour before the test. Each animal one by one placed in the cylinder with water, diameter 18 cm, height 40 cm, i.e., of sufficient size to ensure that the mouse was able to swim freely. Water temperature was maintained within 29-30°C. the Voyage was carried out with the load (lead tube on the rubber ring attached to the root of the tail), equal to 20% of the body weight of the animal. Mouse sailed with a cargo to fatigue, evidenced by immersion of the animal on the bottom of the cylinder. At this point the animal was quickly removed from the water. Average time swimming, which is in direct relation to the endurance of the body to extreme loads, were recorded in minutes from the beginning of the voyage mouse to complete immersion under water.

Example 5. Anti-inflammatory activity of the claimed BAD.

The experiment was carried out in Bezmaternykh mice-males line DM-1 mass of 18±2 g Respecify the definition of the local inflammation induced by the introduction of 250 μg (25 μl of saline) Delta-carrageenan (type IV, Sigma) in the pad of the left hind paws of each experimental animal. In the pad of the right hind paws were administered saline (control). The drugs were administered orally 1 h before the induction of inflammation by carrageenan. Control group animals received saline. As positive control used well-known anti-inflammatory drug Indomethacin". After 5 h, the mice were subjected to euthanasia, feet cut off on the ledge in the bone below the junction of the small and the tibia, and weighed on an analytical balance accurate to 4 digits. The level of slowdown tissue granuloma was calculated by the equation:

Slow inflammation (%)=TA-TV/TA×100,

where TC is the fabric weight of granuloma in the control group; TV - fabric weight of granuloma in the group that received anti-inflammatory drugs.

Example 6. Hepatoprotective and antioxidant effect of the claimed BAD.

Investigation of hepatoprotective properties were conducted on the model of hepatotoxicity induced by carbon tetrachloride (CCl4) in experimental animals (mouse, the male line of the IAS weighing 20±2 g). The study of the hepatoprotective action of the claimed SUPPLEMENTS were performed on four groups of animals (7 animals in each group): 1) intact group; 2) the group of negative control (untreated animals); 3) group is orytelling control (animals, which was introduced by a famous hepatoprotector "Cars" in a dose of 10 mg/kg); 4) experimental group of animals (declare Supplement was introduced to cultivation in 5 times). The drugs were injected animals with the help of the probe into the stomach once a day for 5 days. Two control groups received distilled water.

After 16 h after the last injection of one control group (negative control) and two experimental groups of animals intraperitoneally injected with a mixture of CCl4-vegetable oil (1:1, by volume) at a dose of 300 μl/100 g Control group (intact animals) was administered only vegetable oil.

After 2 h after intoxication CCl4animals were subjected to euthanasia by the method of cervical dislocation and were fences blood. Heparinized blood was centrifuged at 4000 rpm for 15-20 minutes, the resulting blood plasma was used for biochemical tests. The level of total bilirubin, alanine aminotransferase (Alat) and malondialdehyde (MDA) in the blood plasma of the animals were determined using biochemical kits "olivex" (CJSC "olivex", Russia). The results are shown in table 4.

As can be seen from the data presented in table 4, cirrhotic changes in the liver was accompanied by a sharp change of biochemical parameters in blood plasma compared to the group is Oh the intact animal.

On the basis of Toxicological and pharmacological studies of the claimed functional food additives on laboratory mice showed the following.

1. The claimed BAD has a very low acute toxicity (LD50>1 g/kg) and therefore its use is safe in a wide range of concentrations.

2. In the forced swimming test mice on the definition of the tonic action of the claimed SUPPLEMENTS revealed that the composition when diluted 5 times has a strong stimulating effect on the physiological capabilities of the experimental animals, increasing the voyage is almost two times in comparison with control group of animals that did not receive the claimed BAD. It is important to emphasize that we took as a positive control drug "Pantocrine" when breeding 5-10 times is less efficient than the claimed BAD (dilution 5 times). Characteristically, the breeding of the claimed BAD 10 times does not have a stimulating influence on the physiological capabilities of the experimental animals.

3. In the test definition anti-inflammatory activity shows that the claimed SUPPLEMENTS, the oral route is the introduction of more than 5 times more effective than non-steroidal anti-inflammatory agent Indomethacin, was taken as positive control. The data obtained POS is enable to conclude, that the claimed BAD when administered orally has a remarkable anti-inflammatory activity.

4. Determining the effectiveness of the claimed BAD in the test toxic hepatitis induced by carbon tetrachloride showed that BAA shows a pronounced protective effect on all the studied biochemical parameters, surpassing the known hepatoprotective drug "Kars". Judging by the dynamics of changes in the level of MDA in plasma of experimental animals, prophylactic administration of animals claimed BAD leads to sharp changes in the antioxidant status of the organism.

The data obtained indicate the presence of strong antioxidant action of the claimed SUPPLEMENTS, which restores and/or activate the system detoxification of reactive oxygen species and products of lipid peroxidation in animals with experimental toxic hepatitis.

Thus, the pharmacological tests allow us to conclude that the proposed biologically active food Supplement strengthens adaptive and protective forces of an organism, increases the ability of organs and tissues to restore, strengthen the vitality of the whole body and slows down ageing. SUPPLEMENTS can be useful as geriatric and geroprotective tool, as well as vspomogateljno tool in oncological practice.

Biologically active food Supplement, firming adaptive and protective forces of the body, has anti-inflammatory, antioxidant activity, containing components of plant origin and chitosan, characterized in that as component of plant origin contains a comprehensive extract of the roots zamanihi high, the roots of leuzea root red root, roots and leaves of celery, root Golden root, the roots of aralia high, the roots of bosnjaci Russian, grass sainfoin sandy, schisandra fruit and additionally contains the enzymatic hydrolysate of trepang, ascorbic acid, taurine, glutathione, nicotinamide, vitamin B1, vitamin B2, vitamin B6, vitamin B12, folic acid, calcium chloride anhydrous, magnesium chloride, zinc chloride, honey bees in the following ratio of ingredients, in kg per 100 gave:

Chitosan10,0-15,0
Ascorbic acid30,0-35,0
Taurine0,1-0,15
Glutathioneof 0.15-0.2
Nicotinamideof 0.15-0.2
Vitamin B1 of 0.15-0.2
Vitamin B2of 0.15-0.2
Vitamin B6of 0.15-0.2
Vitamin B120,04-0,06
Folic acid0,05-0,06
Calcium chloride anhydrous3,6-4,0
Magnesium chloride1,0-1,4
Zinc chloride0,65-0,7
Honey bee700,0-750,0

and l:
A comprehensive extract of the roots zamanihi high
the roots of leuzea root red root,
roots and leaves of celery, root Golden root,
the roots of aralia high, the roots of bosnjaci Russian
grass sainfoin sandy, schisandra fruit6,0-10,0
Enzymatic guide olisat trepang 134,0-136,0

to obtain a comprehensive extract using ethyl alcohol to 39.5-40.5 percent and complex extract contains components in the following ratio in kg per 100 gave extract:
The roots zamanihi high12,0-14,0
The roots of leuzea12,0-14,0
Root red root12,0-14,0
The roots and leaves of celery12,0-14,0
The roots of Golden root12,0-14,0
The roots of aralia high12,0-14,0
The roots of bosnjaci Russian12,0-14,0
Grass sainfoin sandy12,0-14,0
The fruits of schisandra12,0-14,0
Ethyl alcohol330,0-340,0
Distilled waterrest



 

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2 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to the pharmaceutical industry, namely to an agent with an antidyslipidemic and analgesic effect. The method for preparing the phytocomplex with the antidyslipidemic and analgesic effect, involving: a) grinding peeled bergamot fruit to prepare an undegraded mixture, b) introducing pectinolytic enzymes into the mixture; c) reducing pulp content; d) inactivating the above enzymes added at the stage b), to prepare a degraded mixture; e) performing ultrafiltration of the degraded mixture through membranes isolating the substances having a molecular weight of over 30,000 Da, to prepare a transparent solution; f) introducing the transparent solution on a polyphenol absorption column; g) washing the polyphenol absorption column with water and increasing pH to prepare an aqueous polyphenol fraction; h) transmitting the aqueous polyphenol fraction to cationic resin to recover the phytocomplex in an aqueous phase; i) drying the phytocomplex in the aqueous phase. The phytocomplex in the aqueous phase with the antidyslipidemic and analgesic effect. The phytocomplex with the antidyslipidemic and analgesic effect. A pharmaceutical composition with the antidyslipidemic effect containing the phytocomplex, and pharmaceutically acceptable additives. A pharmaceutical composition with the analgesic effect containing the phytocomplex, and pharmaceutically acceptable additives.

EFFECT: phytocomplex described above possesses the evident antidyslipidemic and analgesic effect.

11 cl

FIELD: medicine.

SUBSTANCE: method involves preliminary intraperitoneal single administration of 5% aqueous alloxan in a dose of 15 mg/kg of body weight into a rat's body on an empty stomach. That is followed by administering afobazol under conditions of oxidative stress after observing the rat's blood glucose gain at least twice. Afobazol is administered subcutaneously in a dose of 10 mg/kg of body weight once a day for 30 days with underlying administration of L-arginine in a dose of 10 mg/kg of body weight or with underlying NG-nitroarginine methyl ester (L-NAME)-inhibitor of NOS-3 enzyme in a dose of 25 mg/kg of animal's weight.

EFFECT: method enables correcting the oxidative stress and NO-producing endothelial dysfunction accompanying vascular complications of diabetes mellitus.

1 dwg, 6 tbl, 1 ex

FIELD: medicine.

SUBSTANCE: invention concerns an antioxidant representing the amino acid glycine immobilised on the detonation-synthesised nanodiamond particles of 2-10 nm in size.

EFFECT: higher efficacy.

4 cl, 5 dwg, 7 tbl, 3 ex

FIELD: medicine.

SUBSTANCE: improving the functional result of a low resection of rectum in the patient suffering from rectum cancer is ensured by prescribing the drug preparation Laviocard+ 1 capsule 2 times a day with food for the pre-operative radiation course, one day before the operation and for 30 postoperative days to the extent of the low resection of rectum.

EFFECT: invention enables reducing a rate and degree of defecation and continence dysfunctions following the low resection of rectum and the radiation therapy.

1 tbl, 1 ex

FIELD: chemistry.

SUBSTANCE: invention relates to compound for formula (1) , antioxidant, containing formula (1) compound or its salt as active ingredient, and to application of formula (1) compound or its salt for oxidant manufacturing. Invention also relates to formula (2) compound, which is intermediate for obtaining formula (1) compound.

EFFECT: formula compound, demonstrating antioxidant properties.

4 cl, 1 tbl, 13 ex

FIELD: chemistry.

SUBSTANCE: invention relates to medication, representing betulin bis-isonicotinoate .

EFFECT: low toxicity, expressed anti-ulcer, anti-inflammatory and hepatoprotective activity.

3 tbl, 5 ex

Jak inhibitors // 2538204

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to compounds of formula I wherein R means C1-6alkyl, C1-6halogenalkyl, hydroxy-C1-6alkyl, hydroxygroup or halogen; m, n is equal to 0 or 1; Z1 means CH or NH; Z2 means CH or N; Z3 means CR1, N or NR2; R1 means H, C1-6alkyl, C3-7cycloalkyl, cyanogroup, cyano-C1-6alkyl or halogen; R2 means H or C1-6alkyl; X means CH, CR' or N; X' means CH, CR' or N; r is equal to 1; Y means CH or CR'; R' means R'a or R'b; R'a means a halogen or cyanogroup; R'b means C1-6alkyl, heterocycloalkyl specified in piperazinyl, morpholinyl, piperidinyl, thiomorpholinyl, azetidinyl, pyrrolidinyl, OR", SR", S(=O)2R" or NR"R", optionally substituted by one or more R'c; R'c means a hydroxygroup, oxogroup, cyanogroup, C1-6alkyl, pyridinyl, carboxy-C1-6alkyl, aminocarbonyl-C1-6alkylaminogroup, C1-6alkylaminogroup, C1-6dialkylaminogroup or C1-6alkoxygroup; R" means H, C1-6alkyl, hydroxy-C1-6alkyl, piperidinyl, C3-7cycloalkyl or pyridinyl; Q means S(=O)2Q1, C(=O)Q2, C(=O)OQ3 or Q4; Q1 means C1-6alkyl, C3-7cycloalkyl-C1-6alkyl, C1-6alkylaminogroup or C1-6dialkylaminogroup optionally substituted by one or more Q1'; each Q1' independently means C1-6alkyl or cyanogroup; Q2 means C1-6alkyl optionally substituted by one or more Q2'; each Q2' independently means a cyanogroup; Q3 means C1-6alkyl; Q4 means C1-6alkyl, oxetanyl optionally substituted by one or more Q4'; each Q4' independently means a halogen, cyanogroup, cyano-C1-6alkyl; p is equal to 0, 1 or 2; q is equal to 1 or 2; each means a single bond or a double bond; provided one of Z1 and Z2, and Z3 and Z3 bonds are double and single.

EFFECT: compounds of formula I as JAK inhibitors.

23 cl, 2 tbl, 121 ex

FIELD: chemistry.

SUBSTANCE: invention relates to novel derivatives of N-(5-brompyridyl)amide of 4-aryl-4-oxo-2-(oxo-1,2-diphenylethylidene hydrazone)butanoic acid of general formula R=Me (1), MeO (2), Cl (3), having analgesic action.

EFFECT: obtaining novel compounds with useful biological activity.

1 tbl, 4 ex

FIELD: medicine.

SUBSTANCE: invention relates to field of veterinary and is intended for treatment and prevention of postpartum acute mastitis in cows. Medication contains antimicrobial, anti-inflammatory preparations and distilled water. Medication additionally contains licorice root. As antimicrobial preparation it contains metronidazole. as anti-inflammatory preparation it contains ciprofloxacin, with the following component ratio, g/l: licorice root 80-85 g; metronidazole 3.5-4.0 g; ciprofloxacin 2.0-2.5 g; distilled water - the remaining part.

EFFECT: application of claimed medication provides expressed antimicrobial and anti-inflammatory effect with simultaneous reduction of organism's response to histamine, release of spasm of smooth muscles and reduction of permeability of capillaries.

4 tbl, 10 ex

FIELD: chemistry.

SUBSTANCE: described is novel biologically active compound 2-methylsulphanyl-6-nitro-7-oxo-1,2,4-triazolo[5,1-c][1,2,4]triazinide L-argininium dehydrate of formula , which has antiviral action, method of its obtaining and application for prevention and treatment of West Nile fever.

EFFECT: increased efficiency of compound application.

3 cl, 2 tbl, 2 ex

FIELD: chemistry.

SUBSTANCE: invention relates to a compound of formula wherein each of R1 and R2 is independently selected from a group consisting of a hydrogen atom, nitro and NR6R7; R3 is C1-C8alkyl; each of R4 and R5 is independently selected from a group consisting of C1-C8alkoxy, phenoxy and phenyl(C1-C8alkylene)oxy; each of R6 and R7 is independently selected from a group consisting of a hydrogen atom, C1-C8alkyl, C(O)R8 and SO2R8;R8 is selected from a group consisting of a hydrogen atom, C1-C8alkyl, halogen-substituted C1-C8-alkyl, C1-C8-alkyl, substituted (C1-C8-alkylsubstituted amino), C1-C8-alkyl, substituted with piperidine and C1-C8-alkyl, substituted with morpholine.

EFFECT: reduced PDE4 enzyme activity and treating PDE4 enzyme mediated diseases or conditions.

21 cl, 2 tbl, 32 ex

FIELD: chemistry.

SUBSTANCE: invention relates to novel cocrystals of niflumic acid with isonicotinamide or caffeine, where molar ratio of niflumic acid with isonicotinamide or caffeine constitutes 1:1, and cocrystal of niflumic acid with isonicotinamide has entothermic peak from 152 to 162°C according to the data of measurement by means of differential scanning calorimetry and peaks at 2θ(°) 6.3, 7.4, 12.5, 14.5, 19.2, 23.2, 25.0 by the data of measurement of X-ray radiation difraction on powder, and cocrystal of niflumic acid with caffeine has endothermic peak from 155 to 165°C by the data of measurements by means of differential scanning calorimetry and peaks at 2θ(°) 9.7, 12.0, 13.26, 14.3, 17.0, 18.1, 22.5, 26.2 and 26.9 by the data of measurement of X-ray radiation diffraction on powder.

EFFECT: increase of solubility in water in comparison with indices of niflumic acid solubility in pure form.

12 dwg, 2 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to compounds of formula (I) and (II), which possess the blocking activity on voltage-sensitive sodium channels, such as TTX-S channels, and their pharmaceutically salts. In general formula (I) and (II), R1 represents -CF3, -CHF2, -OCF3, -OCHF2, -OCH2CHF2/ -OCH2CF3, -OCF2CHF2; -OCF2CF3, -OCH2CH2CF3, -OCH(CH3)CF3, -OCH2C(CH3)F2, -OCH2CF2CHF2, -OCH2CF2CF3, OCH2CH2OCH2CF3, -NHCH2CF3, -SCF3, -SCH2CF3, -CH2CF3 -CH2CH2CF3, -CH2OCH2CF3 and -OCH2CH2OCF3; R2 is specified in (1) hydrogen, (2) halogen (3) -On-C1-6 alkyl, (4) -On-C3-6 cycloalkyl, (5) -On-phenyl, (6) -On-heterocyclic group, (7) -NR7 (C=O)R8; wherein n is equal to 0 or 1, p is equal to 1, 2; R3 and R4 represents hydrogen or C1-6 alkyl, X represents carbon atom; Y represents hydrogen or C1-6 alkyl; Ar represents 4-pyridyl, 4-pyrimidyl or 6-pyrimidyl, which is substituted in the 2nd position by a substitute, which is independently specified in (1) -(C=O)-NR7R8, (2) -NR7(C=O)R8; R9 is specified in: (1) hydrogen, (2) halogen, (3) -On-C1-6 alkyl, wherein alkyl is unsubstituted or substituted by hydroxyl; q is equal to 1, 2 or 3; R10 independently represents hydrogen, C1-6 alkyl, C2-6alkenyl, C3-7 cycloalkyl or phenyl, which is unsubstituted or substituted by one or substitutes independently specified in hydroxyl, -On-C1-6 alkyl and -C3-7 cycloalkyl.

EFFECT: preparing the compounds possessing the blocking activity on voltage-sensitive sodium channels.

7 cl, 2 tbl, 1281 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to a new compound, namely to (S)-enantiomer of 1'-{[5-(trifluoromethyl)furan-2-yl]methyl}spiro[furo[2,3-f][1,3]benzodioxol-7,3'-indole]-2'(1'H)-one of formula (I), and a method for preparing it which is effective for treating diseases and conditions, such as pain, an intensity of which can be reduced or relieved by modulating potential-dependent sodium channel gatings.

EFFECT: invention refers to the pharmaceutical composition of the above compound, methods of treating and a method of relieving an ion flux through the potassium channel gating in a cell.

10 cl, 5 tbl, 6 dwg, 11 ex

FIELD: medicine.

SUBSTANCE: agent possessing the anti-inflammatory, antipyretic and antimicrobial action representing a dry extract of drug hedge hyssop leaves and blossom by grinding them, extracting in 96% alcohol on a water bath to a boil, and boiling, evaporating, diluting the evaporated residue by distilled water first, adding chloroform then, cooling to a room temperature and centrifuging, separating a water fraction and drying it in the certain environment.

EFFECT: agent possesses the pronounced anti-inflammatory, antipyretic and antimicrobial action.

5 dwg, 5 tbl, 2 ex

FIELD: pharmacology.

SUBSTANCE: invention relates to compositions from green tea, stabilised by natural antioxidants, such as oligomer procyanidins (OPC) together with vitamin C or analogues of vitamin C. It is proposed to use a mixture of OPC and ascorbic acid for improved bioavailability of green tea catechines. The method includes a stage of using the composition containing a green tea catechine and a mixture of oligomer procyanidins (OPC) and ascorbic acid or analogues of the vitamin C.

EFFECT: increased bioavailability of catechines in food or cosmetic compositions.

12 cl, 4 dwg, 11 tbl, 2 ex

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