Method of applying vegetable polysaccharide as radioprotector and stimulator of colony formation of spleen stem cells in irradiated animals
SUBSTANCE: invention relates to radiation and experimental biology, biotechnology and medicine, namely to means of radiological protection and to stimulators of colony formation of stem cells. Invention can be used in research and clinical practice for protection of living organisms in case of exposure to ionising radiation, in emergency and military medicine, in case of emergency situations as means for protection against radiation injury. Claimed is application of preparation gamma-plant as radioprotector with colony-stimulating properties for increasing survival and improving state of organisms, subjected to irradiation. Gamma-plant is non-toxic polysaccharide of vegetable origin, registered by Ministry of Health of the Russian Federation and allowed for medical application; Gamma-plant is included into Russian Pharmacopoeia in section FG-9 "non-narcotic analgesics".
EFFECT: invention makes it possible to increase efficiency of colony formation of stem cells in spleen 2,0-2,3 times, increases survival of experimental animals to 95% with coefficient of dose reduction factor DRF, equal 2-2,2.
The invention relates to radiation and experimental biology, biotechnology and medicine, in particular to the means of radiotoxic and stimulants colony of stem cells can be used in research and clinical practice for the protection of living organisms when exposed to ionizing radiation, emergency and military medicine, in emergency situations as a means of protection from radiation damage.
The invention consists in the use of plant polysaccharide gamma-plant (Czekanowska L. A. Polysaccharide gamma-plant (γ pl), having anti-infective activity and not having hemaglutinin action. EN 2028303 C1) as a radioprotector and stimulant colonies of stem cells in the spleens of irradiated animals.
Currently there is an acute problem of protection from radiation injuries. To mitigate exposure use radioprotectors. To radioprotectors are substances that are capable for prophylactic use to have a protective effect, resulting in the preservation of life is irradiated to the living body or the weakening of the severity of radiation injury with rollover state capacity and lifetime (Century. And. Legeza, V., Vladimirov. A new classification of preventive radioprotection means. the radiation biology. Radioecology, T. 38, vol.3, page 416, 1998). Basic requirements radioprotectors, are that they should be quite effective and do not cause adverse reactions. Known radioprotectors in full these requirements are not responsible and the selection of biologically active substances with radiomodification activity is limited.
Known radioprotectors - hamatos and tsistamin included in military first aid kit AI-1,2,3 Thu, 4 with FOOD (to reduce the absorbed dose) of 1.6 and 1.15, respectively. (Swerdlow and collaborators 1974); They are radioprotectors short-term actions are considered to be the most effective. The disadvantage is widely used in the practice of the radioprotector of applied Kuna P. Chemical radiocasete. TRANS. with the construction. M.: Medicine, 1989, S. 61-75) is that its effect is observed at doses that are close to toxic. The use of these doses is in laboratory animals acute side effects: low blood pressure, bradycardia, decreased cardiac output with disturbance of blood supply to tissues. Have nausea, vomiting, defecation, skeletal muscle cramps, death through paralysis of the respiratory center.
Other known radioprotectors - biogenic amines have FOOD to 1.3-1.5, also have a number of serious side action is s: cause a spasm of the blood vessels and circulatory changes in the blood supply in radiosensitive organs and tissues (C. A. Kutsenko. Military toxicology, radiobiology and health protection. SPb: LLC "Publishing house" FOLIO", 2004). The use of these drugs is limited due to the difficulty to establish the optimal dose, time and route of administration.
Common deficiencies the most effective of the known radioprotectors should also be noted that their radioprotective properties, mainly manifested when used before exposure. In extreme situations, in particular in case of accidents at nuclear installations, the use of nuclear weapons by the enemy, when to enter the drug should after exposure, the protective effect is significantly reduced (Shashkov B. C. Anashkin O. D. Suvorov, N. N. Manaeva I. A. the Effectiveness of serotonin, maxamine, AET and applied with the re-introduction after gamma irradiation. // Radiobiology, 1971, T. XI, vol.4, S. 621-623).
There is a method of colony-stimulating stem cells in the spleen after exposure to drugs of IL-1a and IL-1b in a dose of 1 μg/animal after 1 or 3 h after radiation exposure [Chigarev N. G. Interleukin-1b as a means early treatment of acute radiation sickness / N. G. Chigarev, A. F. Myasoedov, N. In. Petkevich, etc. // Fundamental and applied problems of biotechnology and medicine: abstracts. Dokl. nauch.-technology. proc. - SPb., 2000. - S. 31]. It is shown that preparations of IL-1 had a positive effect on the blood, which then manifested itself in the increase of the content of formed elements in the peripheral blood, the increase in the number of myelokaryocytes in the bone marrow, increasing the number of colony forming units in the spleen [Legeza Century. And. cytokines as a means early pathogenetic therapy of radiation injuries. Efficacy and mechanism of action / Century And. Legeza, N. G. Chigarev, Y. A. Abdul, I. W. Galeev // Radiat. biology. Radioecology. - 2000. - So 40, No. 4. - S. 420-424]. In the literature a large number of papers indicate that the most promising drug for early treatment of radiation injuries in Russia is recombinant IL-1β (betalain). Preparation betalain, which dosage form of recombinant IL-1β person authorized by order of the Ministry of health of the Russian Federation No. 51 from 18.02.1997, to clinical application as an immunostimulant and as a means of recovery of bone marrow hematopoiesis in cancer patients after intensive courses of chemo - and radiotherapy (registration number 97/51/6). The disadvantage of this method is the use of pharmacological drug has certain contraindications for use. IL-1 is a proinflammatory cytokine secreted by the cells of the body, and its level in the blood has a fairly broad meaning, which cannot be considered in emergency situations. Errors in establishing the optimal dose, time and route of administration lead to severe side effects - immune disorders the regulation, stimulation reaction, inflammation, high cytotoxicity; application causes fever, sweating, headache. A strong increase in the level of IL-1 leads to hypotension, anorexia, destruction of cartilage in joints. IL-1 is involved in regulation of body temperature, increased its production leads to the development of fever. In addition, there are a number of diseases, the pathogenesis of which has increased levels of IL-1 and application of therapy cytokines are contraindicated.
The purpose of this invention is to develop a method that allows the use of polysaccharide of plant origin of gamma-plant for radiation protection, ensuring a higher survival of living organisms before and after exposure to radiation damage, which will give the opportunity not only to expand your Arsenal of radioprotective substances, but also to find the most effective connection with no toxicity.
The problem is solved in that the detected new radioprotective properties of the drug gamma-plant, natural non-toxic polysaccharide (drug Gamma-plant" was registered by the Russian Ministry of health and approved for medical use and industrial production. Registration certificate of Ministry of health of the Russian Federation No. 99/47/12 February 10, 1999 Registration certificate of Ministry of health of the Russian Federation No. 99/47/1 February 10, 1999, In the Case lcars the governmental funds of Russia "Gamma-plant" is listed in the section FG-9 "analgesics" on page 386 edition 2001), tested dose and the way of its introduction, before and after irradiation of animals on a background of reception of stimulation colonies of stem cells in irradiated animals. When this is achieved: high value FOOD 2-2,2, stimulation of growth of colonies of stem cells in 2-2,3 times compared to non-irradiated animals and increase the survival rate of 95%.
Gamma-plant is a glycoprotein containing up to 95% of carbohydrates and not less than 0.5% protein with high molecular weight isolated from plant cells. Dosage form gamma-Planta presented in ampoules in a 0.01% solution for injections. Latitude therapeutic range gamma-Planta more than 1000.
To perform the tasks carried out an impact assessment of gamma-Planta on the survival of irradiated animals - males line F1(CBA×S57B1/6) weighing 20-25 g of the Compound was administered intravenously in doses of 1, 10, 100 γ/animal. Radioprotective activity of the compounds was studied at a dose of 800 rad (LD80/30). Irradiation of animals produced at facilities IGUR. The control were only irradiated animals. Within 30 days were observed for General condition and behavior of animals. Control animals differed low activity, poor appetite, loss of hair, was also observed dyspeptic syndrome. Animals with the introduction of the gamma-Planta showed great is th activity, normal appetite and no loss of hair.
The evaluation of the preventive radioprotective properties of gamma-Planta experimental animals were injected intravenously 1 hour before radiation exposure dose of 800 rad (dose 158 rad/min, the exposure time 5 min 4 sec).
To evaluate therapeutic radioprotective properties of gamma-Planta experimental animals were subjected to radiation at a dose of 800 rad (dose 158 rad/min, the exposure time 5 min 4 sec), after which he entered the gamma-plant intravenously through 1.6 and 24 hours.
The results are shown in table 1.
|The results of the experiment for the evaluation of the radioprotective activity of gamma-Planta in mice by intravenous method of administration in the conditions of acute exposure.|
|Time||The dose of the drug, γ/animal||The number of animals||Survived|
|After 1 hour||1||20||16||80|
|After 6 hours||1||20||19||95|
|After 24 hours||1||20||16||80|
|At this dose, the survival rate of mice in the control groups was equal to 20%.|
The results indicate that under these conditions the compound gamma-plant has radioprotective activity. Studied doses of 1, 10 and 100 μg/animal have the same activity, which further increases therapeutic latitude. Draws the attention of high therapeutic radioprotective effect of gamma-Planta during the day - this is a very important quality of the connection, which gives the ability to provide emergency assistance in almost any point of the globe. The use of gamma Planta as a radioprotector provides high radioprotective effect, it was hypothesized that this effect is based on the stimulation of growth of colonies of hematopoietic stem cells.
Gamma-plant prepared from a source of sterile solution containing 100 µg of dry polysaccharide in 1 ml of physiological solution. In the experiments used a solution with a concentration of 10 μg/0.2 ml, which was prepared immediately before the experience after dilution of the initial solution of sterile saline. Was administered once.
To assess stimulating action of polysaccharide schemes the-plant on kolonialapologie hematopoietic precursor cells in the spleen before or after irradiation experimental studies were performed on mice-hybrids F 1(CBA×S57In1/6), males aged 3 months, weighing 20-22 g
The evaluation of the preventive effects of gamma Planta experimental animals for 1 hour and 3 hours before irradiation was injected intravenously 10 µg/animal polysaccharide. All animals were subjected to gamma irradiation (source of Co60) at a dose of 6 Gy on the device "Ray" (dose - 52,63 with Gr/min, irradiation time - 11 min 24 sec).
To determine therapeutic effects of gamma Planta animals were first subjected to gamma irradiation (source of Co60) at a dose of 6 Gy on the device "Ray" (dose - 52,63 cGy/min, irradiation time - 11 min 24 sec), and then experimental animals after 1 hour and 24 hours after irradiation was injected intravenously 10 µg/animal polysaccharide.
All experimental groups of animals were compared with control group of animals, in which mice were injected with saline under the scheme the introduction of the polysaccharide. All animals were subjected to gamma irradiation (source of Co60) at a dose of 6 Gy on the device "Ray" (dose - 52,63 cGy/min, irradiation time - 11 min 24 sec).
On the eighth day after exposure, all animals were euthanized by injection of a solution of Nembutal from a rate of 60 mg/kg body weight, were extracted from the spleen, which was weighed and placed for fixation in freshly prepared solution of acetic acid alcohol (a mixture of 3 parts 95% ethyl sleep the TA and 1 part glacial acetic acid). After 2 hours of fixation of the spleen were transferred to 70% ethanol, and were counted formed splenic colonies on the surface of spleens with a diameter exceeding 0.2 mm, the Average number of formed colonies and standard error (M±M) was calculated on the basis of guidelines prepared by the Ministry of health of the USSR assessment in radiobiological experiments survival of CFU-S (colony forming units splenic), 1975
Criterion stimulating action of polysaccharide gamma-plant was the formation of colonies in the spleens of irradiated animals. The number of colonies is proportional to the number of surviving precursor cells.
The results are shown in table 2.
|The results of the experiment for the evaluation of the radioprotective activity of gamma-Planta (SE) on the test of survival of hematopoietic stem cells that form the splenic colonies (CFU-C), 8 days after irradiation of mice hybrids F1(CBA×C57B1/6)|
|no group||Group||Weight of spleen (mg M±M||The number of CFU-C in the group, M±M|
|1.||Control, irradiation of 6 Gy||31,5±1,5||0,8±0,1|
|2.||Introduction SE for 3 h to 6 Gr||32,1±0,5||1,6±0,2∗|
|3.||The introduction of a GP for 1 h to 6 Gr||33,8±1,8||1,9±0,4∗|
|4.||Introduction SE after 1 h after 6 Gy||34,7±1,4||1,9±0,3∗|
|5.||The introduction of the GP within 24 h after 6 Gy||33,0±1,3||1,6±0,3∗|
|∗ is statistically significant (p<0.05) difference with the index of the control group by the number of CFU-S.|
The table shows that all the intervals of polysaccharide gamma-plant are stimulants hematopoietic stem cells.
A single injection to mice of the polysaccharide before irradiation increases the number of colonies in 2-2,3 times, with the greatest preventive effect was observed in the group in which gamma-plant was administered one hour before irradiation.
A single injection to mice of the polysaccharide after irradiation also increases the number is about colonies in 2-2,3 times, with the greatest therapeutic effect was observed in the group in which gamma-plant was administered one hour after exposure.
Examples of specific performance:
Example No. 1.
Studies were performed on mice-hybrids F1(CBA×C57B1/6), males aged 3 months, weighing 20-22 g Were used 5 groups of mice containing 12 animals in each group.
1st group (control) animals were injected with saline.
2nd group - mice were administered 10 μg of gamma-Planta in a volume of 0.2 ml for 3 hours before irradiation.
3rd group - mice were administered 10 μg of gamma-Planta in a volume of 0.2 ml for 1 hour before irradiation.
4-I group - mice were administered 10 μg of gamma-Planta in a volume of 0.2 ml 1 hour after irradiation.
5th group - mice were administered 10 μg of gamma-Planta in a volume of 0.2 ml 24 hours after exposure.
Gamma-plant immediately before the experience bred from the original sterile solution containing 100 µg of dry polysaccharide in 1 ml of physiological solution, to a solution with a concentration of 10 μg/0.2 ml sterile saline and administered to mice once intravenously into the tail vein or subcutaneously.
Then the animals are subjected to gamma irradiation (source of Co60) at a dose of 6 Gy on the device "Ray" (dose - 52,63 cGy/min, irradiation time - 11 min 24 sec).
On the eighth day after irradiation anyone alive who Tim was performed euthanasia by injection of a solution of Nembutal from a rate of 60 mg/kg body weight, were extracted from the spleen, which was weighed and placed for fixation in freshly prepared solution of acetic acid alcohol (a mixture of 3 parts 95% ethyl alcohol and 1 part glacial acetic acid). After 2 hours of fixation of the spleen were transferred to 70% ethanol, and were counted formed splenic colonies on the surface of spleens with a diameter exceeding 0.2 mm
The results of the experiment are presented in table 2.
The table shows that the introduction of polysaccharide gamma-plant increases the number of endogenous colonies in the spleen of 2.0-2.3 times.
The observed increase in the number of colonies in the spleens of irradiated animals is associated with an increase in the number of survivors of hematopoietic precursor cells. This indicates the presence of the investigational product's ability to be not only therapeutic, but also radioprotective action on stem cells from ionizing radiation.
Example # 2
Studies were performed on mice-hybrids F1(CBA×C57B1/6), weighing 20-25 g Were used in 13 groups of mice containing 10 animals in each group.
1st group (control) animals were injected with saline.
2nd group - mice was injected intravenously at 1 mg gamma-Planta in a volume of 0.2 ml for 1 hour before irradiation.
3rd group - mice intravenously injected p is 10 µg gamma-Planta in a volume of 0.2 ml for 1 hour before irradiation.
4th group of mice was injected intravenously at 100 µg gamma-Planta in a volume of 0.2 ml for 1 hour before irradiation.
5th group of mice was injected intravenously at 1 mg gamma-Planta in a volume of 0.2 ml after 1 hour after irradiation.
6th group of mice was injected intravenously at 10 mg gamma-Planta in a volume of 0.2 ml after 1 hour after irradiation.
7th band - mice intravenously injected 100 μg gamma-Planta in a volume of 0.2 ml after 1 hour after irradiation.
8th group of mice was injected intravenously at 1 mg gamma-Planta in a volume of 0.2 ml after 6 hours after exposure.
9th group of mice was injected intravenously at 10 mg gamma-Planta in a volume of 0.2 ml after 6 hours after exposure.
10-th group of mice was injected intravenously at 100 µg gamma-Planta in a volume of 0.2 ml after 6 hours after exposure.
the 11th group of mice was injected intravenously at 1 mg gamma-Planta in a volume of 0.2 ml 24 hours after exposure.
the 12th group of mice was injected intravenously at 10 mg gamma-Planta in a volume of 0.2 ml 24 hours after exposure.
13th group of mice was injected intravenously at 100 µg gamma-Planta in a volume of 0.2 ml 24 hours after exposure.
Gamma-plant immediately before the experience bred from the original sterile solution containing 500 μg of dry polysaccharide in 1 ml sterile saline to concentrations of 1 and 10 μg/0.2 ml sterile physio is ogicheskom solution. The original solution was a concentration of 100 µg/0,2 ml
The resulting solutions were injected into mice once intravenously into the tail vein.
Then animals were irradiated at facilities IGUR at a dose of 800 rad (dose 158 rad/min, the exposure time 5 min 4 sec)).
Within 30 days were observed for General condition and behavior of animals, and then evaluated the survival of animals of all groups.
The results are shown in table 1.
The introduction of this substance as a radioprotector for the needs of nuclear power plant workers, rescue workers, medical workers and the military will allow to raise efficiency of their work in case of emergencies or when interacting with radioisotopes, providing more than double the value of FOOD in the absence of negative side effects, including toxic.
The use of the drug gamma-plant as a radioprotector with distinct colony-stimulating properties to increase survival and improve the condition of the organisms exposed.
SUBSTANCE: invention refers to veterinary science, namely to clinical pharmacology and veterinary therapy. The method consists in administering the complex iron-dextran preparation Ferranimal-75M intramuscularly on the 5th day of calf's life in a dose of 3 ml in a combination with an intramuscular injection of the preparation Hydropeptone in a dose of 10 ml. Ferranimal-75M is injected 10 days later in a dose of 2 ml in a combination with an injection of Hyropeptone 5 ml intramuscularly in different points.
EFFECT: method provides higher antioxidative activity of calf's blood serum, reduced pro-oxidant action of iron and incorporated radionuclides, as well as higher iron accessibility in treating and preventing iron-deficiency anaemia in calves exposed to the chronic incorporated radiation.
SUBSTANCE: first, Fraxiparine is inhaled for 5-10 minutes. A dialysis solution for peritoneal dialysis containing extranyl is administered into the abdominal cavity 1-1.5 hours later through a catheter. The solution additionally contains: albumin in the concentration of 35-40 g/l, heparin in the concentration of 500-750 Unit/l and papaverine in the concentration of 50-100 mg/l. Every 6-8 hours after the beginning of exposure of the dialysis solution in the abdominal cavity, the Fraxiparine inhalations are repeated. The time of a single exposure of the dialysis solution in the abdominal cavity makes 12-18 hours.
EFFECT: method provides effective body detoxification in the given category of patients by providing a high toxin clearance owing to a continuous high osmotic concentration of the solution and the dilatation of pre-capillary peritoneal arterioles, prevented inspissation of the albumin solution to the colloidal state and its inactivation that reduces a rate of the dialysis solution exchanges.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to pharmaceutical industry, namely to an immunomodulatory composition for injection into a mammal. The immunomodulatory composition for injection into a mammal containing a hydrolysate prepared by acid and/or enzymatic hydrolysis of one or more bioresources specified in a group consisting of bivalve molluscs, annelids, leeches, and water taken in certain proportions. A method for preparing the immunomodulatory composition for injection into a mammal. A method of treating a pathological condition in a mammal in need thereof involving the injections of the immunomodulatory composition into the above mammal. Using the composition for normalising metabolism into the mammal in need thereof.
EFFECT: composition enables extending the range of products with immunomodulatory activity for injections.
19 cl, 7 tbl, 7 ex
FIELD: veterinary medicine.
SUBSTANCE: method of treatment of calves with Simuliidotoxicosis is that 40% solution of urotropine is administered intravenously to calves at a dose of 1.5 ml per 10 kg of animal body weight.
EFFECT: improvement of efficiency of the method.
SUBSTANCE: group of inventions relates to veterinary and medicine, in particular to obtaining and application of biopreparations for immunotherapy of ecopathologies. The group of inventions includes obtaining a protein antigen from a mixture of anatoxins from three enteropathogenic virulent strains of a pathogen of escherichiosis of calves E.coli No. 378, 379, 380 by their growing on Hottinger medium with further addition of 0.4-0.5% formalin, following thermostating for 10-12 days and cooling the mixture of anatoxins with a sterile solution of aluminium hydroxide, after that obtaining radioantigen from E.coli "PL-6" by growing cultures on a meat infusion agar with further washing away a biomass by a physiological solution and irradiation of the obtained suspension with a concentration of 1.2·1010 m.c./cm3 on a gamma-installation in a dose of 140-150 Gr with following thermostating and extraction of radiotoxin with 70% acidified with 0.05% hydrochloric acid to pH 5.5 ethanol, following evaporation of the exractant to the initial volume, after that, obtaining protein-cadmium radioantigen first by preliminary dechlorination of cadmium chloride, obtaining cadmium hydroxide, addition into the obtained 2.7% solution of the antigen of 0.77% solution of cadmium hydroxide in a ratio of 1:1 with following thermostating at a temperature of 37°C for 30 minutes, evaporation and dissolution of the residue to 12.8% concentration, further obtaining protein-cadmium radioantigen by an addition of 1.2% solution of a mixture of three anatoxins of E.coli and 12.8% solution of cadmium radioantigen in a ratio of 1:9, conjugation of components at room temperature for 8-10 hours, standardisation by a dry substance to 10% concentration and pouring into vials, and storage at a temperature of 4-6°C. The group of inventions also relates to a method of treating radiation, chemical and/or bacteriological damage to an organism by introduction of 10% solution of complex protein-cadmium radioantigen.
EFFECT: application of the group of inventions is effective in treatment of radiation, chemical and/or biological damage.
4 cl, 6 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine and particularly to preparations for a therapy of oncological diseases, treatment of allergy, prevention and health improvement. The invention represents a method for preparing fraction 4 (ADP-f4) and fraction (ADP-f5) adaptogenic Dorogov's preparations, characterised by the fact that a primary product is a fraction 2 antiseptic Dorogov's stimulator (ADP-f2) to be thermally treated.
EFFECT: implementing the given invention provides the more effective prevention and eliciting anti-stress reactions for the purpose of improving the functional state of organism and increasing a resistance in an activation therapy.
SUBSTANCE: invention refers to medicine and veterinary science and aims at increasing the radioresistance in mammals. In the period of 12 hours to 20-30 minutes before a radiation exposure and immediately thereafter, 0.11% oil preparation of chlorophyll is introduced into the mammals. Linseed oil is used as an oil base. The preparation is introduced in a dose of 11-12 mg/kg intramuscularly.
EFFECT: method enables increasing the radioresistance and survival rate in the mammals.
1 tbl, 1 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: composition for treating oxidative stress comprises ball-shaped lipoic acid or one of salts thereof, and at least one lipophilic medium. The lipoic acid balls represent particles consisting of an inert core (a nucleus) coated with lipoic acid which is coated with a first layer of an isolating polymer, and with a second polymer layer resistant (stable) at gastric pH. What is also described is a preparation for treating oxidative stress with an unified dose containing the above composition. The preparation is presented in the form of a soft gelatin capsule.
EFFECT: compositions according to the invention are stable in the lipophilic medium.
22 cl, 15 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: group of inventions refers to medicine, particularly toxicology and radiology, to drug preparations based on antioxidant proteins and methods of using them. The pharmaceutical composition for treating toxic conditions wherein the therapeutic effect is ensured by the action of antioxidant, antimicrobial, antitoxic human lacroferrin protein on the human body contains non-replicating nanoparticles of human adenovirus serotype 5 genome with inserted human lactoferrin expressing human lactoferrin in the therapeutically effective amount in the body, and an expression buffer with the particle content not less than 2.33×1011 of physical particles per ml of the expressing buffer. The method of therapy involves administering the composition in the therapeutically effective dose of 7×1011 of physical particles to 7×1013 of physical particles per ml of the expressing buffer per an individual; the composition is administered intravenously.
EFFECT: invention provides the stable therapeutic effect after the single administration of the composition.
17 cl, 14 ex, 4 dwg
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine, and concerns a pharmaceutical composition for the therapy of acute toxic conditions. The composition for the therapy of acute toxic conditions contains a protein - human lactoferrin, and additionally contains non-replicating nanoparticles with an inserted gene of human lactoferrin, and an expressing buffer in the following proportions per a dose: human lactoferrin 50 to 100 mg; non-replicating nanoparticles - 7×1011 of physical particles; the expressing buffer - the rest, ml. Human lactoferrin is either donor breast milk lactoferrin, or any human lactoferrin.
EFFECT: invention provides fast onset and prolonged antitoxic action.
4 cl, 8 ex, 2 dwg
FIELD: food industry.
SUBSTANCE: invention relates to nutritional compositions based on a natural extract. One proposes the application of an apple extract containing polyphenols for the manufacture of a complete nutritional composition for the reduction of symptoms of allergy caused by food in patients suffering from allergy caused by food allergens. The apple extract is enriched with polyphenols by at least 1.5 times relative to the unpurified apple extract. The composition contains 0.1% - 1 wt % of the apple extract.
EFFECT: proposed composition has no effect on the patients` sensitisation to the said allergens.
13 cl, 6 dwg
SUBSTANCE: claimed invention relates to a water-based composition, suitable for application for personal hygiene, in everyday life and in organisations, which includes: a) certain quantity of a capable of association thickening agent, including a polymer composition, which contains a water-soluble or water-swellable main chain of a synthetic polymer, which contains a bound with covalent bond ends and/or intermediate blocks of oligomer hydrophobic compounds, selected from the group, including I) alkyl and aryl structures, containing a capable of polymerisation cyclic monomer, II) capable of polymerisation double bond, III) derivatives of compounds, listed in I) and II), with blocks representing two or more units of similar or different hydrophobic compounds, b) certain quantity of a surface-active substance, c) certain quantity of a physiologically tolerable salt, selected from the group, including sodium sulphate, potassium chloride and sodium chloride, and d) water; with the quantity of the capable of association thickening agent, contained in the water-based composition constituting from 0.1 to 5 wt %, the quantity of the surface-active substance, contained in the water-based composition, constituting from 5 to 50 wt %, and the polymer composition, including the water-soluble or water-swellable main synthetic polymer chain, includes polyacetal polyether, modified with ethylhexylglycidyl ether (EHGE).
EFFECT: obtaining water compositions based on surface-active substances, demonstrating Newton viscosity at lower shear rates and shear thinning at higher shear rates, with the provision of a possibility to apply different quantities of the surface-active substances, including the reduced quantity of the surface-active substances.
15 cl, 7 ex, 5 tbl, 1 dwg
FIELD: medicine, pharmaceutics.
SUBSTANCE: group of inventions concerns an oral care composition for sensitivity management, and methods using the same. The presented composition contains an orally acceptable carrier, metal oxide particles with an average particle size of no more than a dental tubule size, and a polymer adhesive for metal oxide particle adhesion in the dental tubule, wherein the metal oxide is encapsulated in the polymer adhesive with the metal oxide containing zinc oxide, while the polymer adhesive contains a combination of two polymers of hydroxyalkyl cellulose of various molecular weights, and zinc oxide is encapsulated in a mixture of the two above polymers. There are also presented a method for sensitivity management and a method for dental tubule occlusion on a mammalian dental surface involving coating the dental surface with the above composition.
EFFECT: using the group of inventions provides the effective dental tubule occlusion and sensitivity management, as well as the dental deposit protection and anticalculous effect.
12 cl, 2 ex, 2 tbl, 2 dwg
SUBSTANCE: invention relates to novel compounds of formula (I), which can be applied as an aromatic compound or a smell-masking agent. In formula (I) R stands for a hydrogen atom, C1-C6 alkyl, C2-C6 alkenyl or =CH2; Z stands for CN or CHO; and there is not more than one dashed bond. The following conditions must be observed: if Z stands for CHO and there is one of the dashed bonds, R does not stand for a hydrogen atom, and if there is a dashed bond between carbons Ca and Cb, R does not stand for a group = CH2. The invention also relates to a method of obtaining a formula (I) compound, its application as the aromatic compound or the smell-masking agent, and a perfumery composition.
EFFECT: increased activity of the composition application.
10 cl, 1 tbl, 10 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to novel fluorinated 1,4-naphthoquinone derivatives of general formula , where 1 R=C6H5, X=F; 2 R=2, 5-F2C6H3, X=F; 3 R=CH3, X=F; 4 R=C6H5, X, , possessing cytotoxic activity with respect to cancer cells, which can be applied in medicine.
EFFECT: claimed are novel compounds with anti-cancer activity for therapy of malignant neoplasms.
1 dwg, 3 tbl, 5 ex
FIELD: food industry.
SUBSTANCE: sea-buckthorns seeds based biologically active additive production method envisages seeds sprouting in a wet condition at a temperature of 13-18°C during 12-14 days, drying till moisture content is equal to 14% and milling.
EFFECT: method simplification and cheapening, the additive biological value enhancement, expansion of the range of preventive purpose products and the ready product organoleptic characteristics improvement.
2 tbl, 3 ex
SUBSTANCE: agent possessing the anti-inflammatory, antipyretic and antimicrobial action representing a dry extract of drug hedge hyssop leaves and blossom by grinding them, extracting in 96% alcohol on a water bath to a boil, and boiling, evaporating, diluting the evaporated residue by distilled water first, adding chloroform then, cooling to a room temperature and centrifuging, separating a water fraction and drying it in the certain environment.
EFFECT: agent possesses the pronounced anti-inflammatory, antipyretic and antimicrobial action.
5 dwg, 5 tbl, 2 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to method of estimating antioxidant activity of vegetable raw material from swamp cinquefoil (Comarum palustre L.). Method of estimating antioxidant activity of vegetable raw material from swamp cinquefoil (Comarum palustre L.) consists in determination of antioxidant activity in water tinctures of swamp cinquefoil by reduction of the level of free-radical oxidation, which is determined by the level of malonic dialdehyde (MDA) by method of interaction with thiobarbituric acid in model system of lipid peroxidation, represented by liposomes obtained from lecithin.
EFFECT: method reduces labour intensiveness of antioxidant activity determination and simplifies processing of obtained results, reduces cost of carrying out analysis, and increases determination accuracy.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to the pharmaceutical industry, in particular to a medication, possessing a nephroprotective action. The medication with the nephroprotective action, contains an extract of grass with roots and rhizomes of slender-leaf iris (Iris tenuifolia), stabilising agents and preservatives, taken in a certain ratio, where the extract is obtained by extraction of the grass with roots and rhizomes of slender-leaf iris (Iris tenuifolia) with a 30-70% water solution of polyol.
EFFECT: medication possesses an effective nephroprotective action.
3 cl, 3 dwg, 7 tbl, 14 ex.
FIELD: veterinary science.
SUBSTANCE: preparation of local therapy contains pine resin, chlorophyll carotin paste, birch oil, castor oil in the following proportions: pine resin: chlorophyll carotin paste: birch oil: castor oil - 10:5:5:80.
EFFECT: using the declared preparation enables the higher therapeutic effect as compared to the existing agents for treating animal skin diseases, including dermatitis.
SUBSTANCE: invention is a composition having antibacterial, immunostimulating, anti-allergic and anti-inflammatory action, containing bacterial waste products useful for human body, in the form of exometabolites and fermentolysis products, characterised in that it is a culture medium of lactic acid bacteria, containing laxarane in an amount of 5-10 g/ml, caseicyne, isracydine or their mixture and lectins in an amount of 0.05-2.5 mol/l, histamine in an amount of 0.8-2.0 mmol/l and monocarboxylic fatty acid with an unbranched chain, namely, acetic acid, propionic acid, butyric acid and valeric acid - in an amount of 10-20 mg/ml.
EFFECT: expanding the range of agents having complementary antibacterial, immunomodulating, anti-allergic and anti-inflammatory action.
4 cl, 5 ex