Biologically active food supplement
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to pharmaceutical industry, namely to a biologically active food supplement. The biologically active food supplement possessing the adaptogenic and tonic action and containing antler reindeer's whole blood, antler concentrate according to Technical Specifications (TU) 9860-010-29734071-11, honey, fruit essence, ascorbic acid and Citrosept Grapefruit taken in certain proportions.
EFFECT: formulation enables preparing the biologically active food supplement possessing the enhanced adaptogenic and tonic action.
2 cl, 8 tbl, 1 ex
The invention relates to the food industry and can be used in the manufacture of food supplements enriched with natural biologically active substances.
A method of producing biologically active product (pantogematogen), including the blood of deer and Sika deer at the time of cutting antlers, defibrinating blood, mixing with 96% rectified alcohol, sugar syrup, fruit essences, ascorbic acid and subsequent pasteurization of the product at 53°C for 1 h 45 min (Cm. "Antler reindeer", B. N. Ranger, N. G. Deev, tutorial, M., "Kolos", 1994, S. 52-53).
However, when defibrinating blood is lost (fibrin) 25% of formed elements (average of the blood of deer and Sika deer), and the loss of total protein is over 24%. In addition, the use of pantogematogen obtained by this method is not valid for patients with intolerance to alcohol-containing preparations, and in the treatment of diseases where alcohol form is contraindicated. In the process of pasteurization required precise temperature control, which complicates the process, and the temperature even a few degrees is associated with coagulation of blood proteins.
There is a method of production of pantogematogen, including the blood of deer and Sika deer, the stabilizer is human blood (62,0 g) and mixed with alcohol (5,5), glucose solution (32,0 g), fruit essences (0.4 g) and ascorbic acid (0.1 g), followed by pasteurization of the product by high-frequency currents for 2 hours at a temperature of 48-50°C (see RF patent №2200016, AK 35/14, publ. 2003.03.10).
However, maintaining such a minor fluctuations in temperature to ensure high quality product requires high-precision control, otherwise the consequences would be similar with the previous analogy, moreover, that this method uses whole blood and visible precipitation (coagulation) of fibrinogen occurs already at 55°C.
Izvestno psychoactive drug "Pantogematogen" including, in wt.%: defibrinating blood antler deer (56-60%), 96% ethanol - 5,2-5,6%, with the addition of sugar syrup - 35-37%, fruit essences of 0.4 - 0.6% and ascorbic acid - 0,09-0,11%. Content defibrinating blood of this product is 56-60% (see RF patent №2008008, AK 35/14, publ. 1994.02.08).
However, defibrinating involved the loss of more than 24% of the total protein of the blood, and used as a preservative ethanol contributes to the denaturation of proteins and in addition limits the scope of application of the drug, in particular, in connection with individual intolerance to the proportion of adult patients alcohol-containing preparations, and also in the treatment of children and treatment of diseases, where alcohol form is contraindicated.
Known biologically active food Supplement, obtained by mixing, wt.%: stable blood reindeer, deer and Yakut horses - 55, antler product in the form of alcoholic extract of antlers (70% solution of ethyl alcohol) - 10, a 30% aqueous solution of flower honey - 35, and represents a liquid syrupy liquid brownish-red color (see RF patent №2368250, IPC A23L 1/30, AK 35/14, AK 35/32, A23L 1/08, A23L 1/302 - prototype).
However, in the manufacture of this biologically active additives used alcoholic extract of antlers, with the extract when it goes only about 3% of dry substances panta, which leads to inefficient use of antler raw materials, and the presence of the alcohol solution in the final product limits the contingent of its consumers. In addition, the stabilization of the blood determines the introduction of environmentally friendly product chemical substances, in varying degrees, deteriorating the quality of the product. As shown by the practical use of this method of preparation of biologically active food supplements, breeding of honey contributes to the acidification of the product by 1.5-3.0% of cases, and the absence in the recipe substances that enhance the taste and prebyvajushhij the smell of blood, psychologically limits the user base, so the AK additive has a texture of a brownish-red liquid with odor and taste of animal blood. It should also be noted that the blood Yakut horses on their biological activity is at a lower level and can't be on this indicator with the blood of deer and reindeer, as the biological activity is determined not so much by the content of essential and nonessential amino acids, but to a greater extent by the presence of growth factors and uncontrolled high content (according to the patent prototype) in a mixture of blood red deer and reindeer biological activity of the product as a whole may be at a low level.
In addition, the geographical dispersion of the habitat of animals analyzed increases ten times the cost of the final product because of large transport costs for capture, storage and transportation of raw materials (the blood of deer, reindeer, and Yakut horses) to merge into a single product. All this ultimately reduces consumer properties of the product and makes it unacceptable cost for the main contingent of consumers (children, pupils, students, the elderly).
It is necessary to develop biologically active food supplements (hereinafter the product), which allows to expand the range of tools (based on antler products and honey) with adaptogenic and tonic effect.
This technical R the result is achieved by in biologically active additive to food with adaptogenic and tonic effect, including blood antler deer antler product and honey, according to the claimed invention, use whole blood antler deer, and as antler-product - concentrate on THE 9860-010-29734071-11 and fruit essence, ascorbic acid and the drug "Citrosa grapefruit" in the following ratio of components, wt.%:
|Drug Citrosa grapefruit"||0,08-0,1|
As whole blood antler deer use whole frozen blood antler deer.
Concentrate (TU 9860-010-29734071-11, RF patent №2461384) it has high biological activity, due to the presence not only of the high content of amino acids, vitamins, macro - and micronutrients and hormones, but Bo is greater degree because of the content of growth factors, with a wide range of biological effects, namely, stimulating or inhibiting mitogenes, chemotaxis and differentiation (maturation) of the cells of the body, providing a rejuvenating effect on the tissues and the whole body: re-run ofine old age or because of disease genes, thereby prolonging the life of cells, increase cell division, targeted to stimulate in them the metabolism. Growth factors stimulate the growth of the child, particularly the development of the musculoskeletal system, which is especially important during adolescence. Insulin-like growth factor (IGF) and epidermal (EGF) growth factors promote cell division and maturation of cells occurs under the action of alpha and beta transforming growth factor (TGF). In addition, transforming growth factor involved in cell proliferation and regulation of the growth of tumor cells, nerve growth factor (NGF) stimulates the regeneration of damaged neuro structures organism. The effect of growth factors should be considered in connection with other stimulants, especially with hormones, for example dehydroepiandrosterone, which also includes part of the antler concentrate. Dehydroepiandrosteron (DHEA) - the "mother hormone" of the body, is the basis on which the endocrine glands produce 27 of hormones, including testosterone and dehydrates theron. The increase of DHEA in the body can not only slow the aging process, but also to improve the condition of patients with cancer, memory loss, osteoporosis, heart diseases and so on
Cooked product does not require pasteurization, as part of the honey needed for the conservation of the number and the drug "Citrosa grapefruit". Honey has preservative (antibacterial) properties, so it is not examined for microbiological safety (see GOST 19792-2001, Honey, specifications and Uniform sanitary and epidemiological and hygienic requirements for goods subject to sanitary-epidemiological supervision (control)" chief II, section 1, p. 5.6).
Drug Citrosa grapefruit" represents an extract of bioflavonoids, grapefruit seed (33%) and vitamin C (the so-called complex of vitamin C). Drug Citrosa grapefruit" contains only herbal ingredients, no alcohol and chemical components. Pharmacological properties of the drug: antifungal (fungicidal and fungistatic), antibacterial, antiviral, widely used in medical practice, has high bioavailability, good tolerability, safety, does not destroy the internal microflora. The product produced by the firm "Chintamani international (Norway), representative office in Russia the AI LLC "Chintamani" (Moscow).
To determine the optimal content of honey is able to neutralize the smell and taste of whole (native and frozen) blood, were tested taste of products containing only honey and blood at a ratio of honey:blood- 30:70; 35:65; 40:60; 45:55; 50:50; 55:45. Studies have shown that signs of the disappearance of the smell and taste of blood were noted in the product containing honey 45-50%. Adaptogenic and tonic properties of products (with a ratio of honey:blood 50:50) from native blood and honey (prototype 1) and frozen blood and honey (prototype 2) and the product of the prototype are shown in table 1.
The studies found that the products made from whole blood antler deer and honey, even without additional enrichment antler product (concentrate) adaptogenic and tonic properties superior to the prototype 7.4-8.4 and the 11.5 and 12.4%, respectively.
In order to enhance the beneficial properties of the experimental product was introduced in the formulation of a new product concentrate on THE 9860-010-29734071-11. To determine the optimal percentage of antler concentrate product has conducted a series of experiments, the research results are reflected in table 2.
According to table 2 it is established that the product content antler concentrate 1,0-20% more than the prototype on adaptogenic action by 28.9-32.9 per cent, on the toning 21.8-27,9%. The optimal number of input antler concentrate should be considered as 0.5 to 1.0% (subject to the necessary cost of expensive antler-product - concentrate).
With the aim of eliminating the process of pasteurization (heat treatment) in the formulation of the claimed product was added drug "Citrosa grapefruit" in different doses, the results of the research are reflected in table 3. According to table 3 shows that in the product, canned drug "Nitrazepam grapefruit" in doses of 0.02 and 0.04 g, the growth of colonies detected at 6 and 10 months, respectively.
During the next month the data samples color from dark cherry moved to brown, appeared putrid smell, thick with flakes consistency with bubbles of fermentation. The product, manufactured by a prototype (without pasteurization) the same pattern was observed on the 30th day of the experiment. Product canned in doses of 0.06 to 0.12 g, for 13-15 months had a dark cherry color, fruity smell and a liquid consistency, while the shelf life of the product in all known registered technical conditions is 12 months.
Later in the course of conducting a series of experiments to establish optimal organoleptic properties for the run of the product identified the optimum content of ascorbic acid and fruit essences is 0,08-0,10 and 0,20-0,40%, because the product contains honey additionally gives a pleasant taste and partially preserves in conjunction with the drug "Nitrazepam grapefruit".
The organoleptic characteristics of the claimed product is presented in table 4.
The content of toxic elements in the claimed product complies with "the Uniform sanitary and epidemiological and hygienic requirements for goods subject to sanitary and epidemiological supervision (control), p. 8.2. (table 6).
Microbiological standards the product complies with the requirements of the Uniform sanitary and epidemiological and hygienic requirements for goods subject to sanitary and epidemiological supervision (control), p. 8.2.3 (table 7).
Definition tonic, adaptogenic properties, and increased motor activity was studied in outbred mice weighing 18-22 g
The first experimental group claimed the product was given in a dose of 0.1 ml/bird. using a probe within 20 days together with the main diet, the second experimental group (prototype) in the same way. Control animals were given saline solution and the basic diet. The research results are reflected in t the blitz 8.
During the study of the biological activity of the analyzed products found that the claimed product is superior to the analyzed samples on adaptogenic and toning action, increases locomotor activity (open field test), pain sensitivity (test "hot plate"), and so on, it should be noted that laboratory animals consumed the product of solid and whole frozen blood, significant differences when conducting biological tests had not.
Tested the claimed product in comparison with the prototype on a group of volunteers (employees of the Institute). The experiment involved 20 people (10 men and 10 women) aged from 27 to 40 years. One group (5 men and 5 women) within 15 days consumed 2 times a day for 25 ml of the claimed product, the second group (5 men and 5 women) took the product, made the prototype in the same schema.
According to the survey participants found that they were noted:
- improving performance in 10 people of the first group and 5 in the second;
- normalization of blood pressure in 9 people first group and in 4 second;
- improved sleep 10 people in the first group and in 6 second;
- improvement of health in 10 people of the first group and in 6 second.
In the end the research Institute of the experiment, Barnaul (February, 2013) according to Rospotrebnadzor was exceeded the threshold, the incidence of SARS and Influenza, the participants in the first experimental group, which consumed the claimed product, had no signs of these diseases (clinically healthy), and the participants of the second group - the 4 people mentioned high temperature, acute rhinitis, cough; 3 persons - normal temperature, chronic rhinitis and cough, 3 people the symptoms were not observed (clinically healthy).
As a result of the experiments it can be argued that the claimed product is superior to the prototype on the toning, adaptogenam, nootropic and biostimulating action.
Taking whole blood antler deer carry out the slaughter of clinically healthy animals or groups of donors using krovopoteriah systems directly connected to the tank. Whole blood not later than 3-4 hours after taking should be used for cooking or product should be frozen at a temperature of 15-20°C. Before use whole frozen blood it is thawed at a temperature of 20-25°C for 15-24 hours.
Before cooking product and mixing of all ingredients blood expanded through the installation of dispersion and gomogenizirovannogo products DG-3. Then to 450.0 g of honey mixed with 5.0 g antler is oncentrate, 2.0 g of fruit essences, 0.8 g of ascorbic acid and 0.8 g of the drug "Nitrosate grapefruit" for 20-25 minutes, after which the obtained substrate type blood - 541,4 g, continuing to stir for another 20-25 minutes. After that, the product is Packed and sealed.
Thus, the declared biologically active food Supplement allows you to expand the Arsenal of tools (based on antler products and honey) with adaptogenic and tonic effect.
1. Biologically active food Supplement with adaptogenic and tonic effect, including blood antler deer antler product and honey, characterized in that use whole blood antler deer as antler-product - concentrate on THE 9860-010-29734071-11 and fruit essence, ascorbic acid and the drug "Citrosa grapefruit" in the following ratio, wt.%:
|Concentrate on THE 9860-010-29734071-11||0,5-1,0|
|Drug Citrosa grapefruit is||0,08-0,1|
|Whole blood antler deer||Rest|
2. Biologically active food Supplement under item 1, characterized in that as whole blood antler deer use whole frozen blood antler deer.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to pharmaceutical industry and represents a skin care agent possessing the antifungal properties, containing propolis, ethanol, and a sulphur-containing component, differing the fact that the sulphur-containing component contains elemental nanodispersed sulphur with the components of the agent taken in certain amounts, wt %.
EFFECT: invention provides extending the range of products possessing a wide spectrum of fungicidal action, and promotes the tissue regeneration processes.
3 cl, 3 ex, 1 tbl
FIELD: veterinary medicine.
SUBSTANCE: agent for treatment of inflammation of udder of cows comprises 10-12 g of crystalline iodine, 100-102 g of polyvinylpyrrolidone and additionally in 1000 g/ml: emulsion wax 10-12; glycerol monostearate 50-52; beewax 10-12; refined sunflower oil 77-78; medical vaseline oil 28-29; glycerol 35-37; purified water - the rest to 1000 ml.
EFFECT: agent enables to carry out for short term the effective treatment of fibrinous, serous and suppurative mastitis and avoid the use of antibiotics and their derivatives, sulphanilamides and nitrofurans and thus to eliminate the negative side effect of the agent on animal body, as well as to reduce the economic cost more than 3 times.
1 tbl, 3 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine, particularly to experimental pharmacology, and can be used as a method for rat sedation with an api-phytocomposition. The method for rat sedation with the api-phytocomposition involving single and daily one-week administration of a sedative agent, which is administered intragastrically through a probe in a dose of 200 mg/kg of animal's body weight, wherein the api-phytocomposition contains honey, bee-bread, lime pollen, propolis, valerian extract in a ratio of 10:2:1:1:1, and represents an aqueous suspension.
EFFECT: method described above is effective for rat sedation and causes no side effects.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to the pharmaceutical industry, namely to an ointment for burns, folliculitis, furunculosis, vasculitis treatment and wound healing. The ointment for burns, folliculitis, furunculosis, vasculitis treatment and wound healing containing: bees wax, line seed oil, kerosene and visceral fat of pig taken in certain proportions.
EFFECT: ointment possesses a biostimulating effect, reduces the wound healing time with no post-therapeutic cicatrisation with good fixation, uniform distribution on the skin surface and ease of use.
9 dwg, 9 ex
SUBSTANCE: method involves simulating endothelial dysfunction in Wistar male rats by daily intraperitoneal introduction of L-nitro-arginine-methyl ester 25 mg/kg/day for 7 days. Endothelial dysfunction is corrected by intragastric introduction of an api-phytocomposition in the form of an aqueous suspension of honey, royal jelly, bee bread, Resveratrol in the ratio of 10:1:1:2. The composition is introduced in a dose of 200 mg/kg of an animal's body weight once a day for 7 days.
EFFECT: method provides synergetic endothelioprotective effects with no side effects observed when treating with synthetic preparations.
SUBSTANCE: method involves simulating hepatitis by 7-day alcoholisation in female rats. A hepatoprotective agent is presented by an api-phytocomposition in the form of an aqueous suspension of honey, lecithin and licorice root extract in the ratio of 10:2:1. The composition is introduced twice a day intragastrically before feeding in a dose of 200 mg/kg of body weight 5 days before ethanol introduction and for 7 days one hour before ethanol introduction.
EFFECT: effective treatment of hepatitis with no side effects.
1 ex, 1 tbl, 1 dwg
SUBSTANCE: immune deficiency is modulated in laboratory animals by single cyclophosphan administration in a dose of 200 mg/kg of body weight (the first day of an experiment). The immune deficiency is corrected by an api-phytocomposition in the form of an aqueous suspension of honey, pollen, propolis, purple Echinacea extract in a ratio of 10:1:1:2. The composition is administered into the laboratory animal intragastrically (through a probe) in a dose of 200 mg/kg daily for 10 days on the second day of the experiment.
EFFECT: synergetic effect and effective correction of the experimental immune deficiency.
1 tbl, 1 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to the pharmaceutical industry, namely to a pharmaceutical composition for treatment of a diabetic ulcer. Application of the pharmaceutical composition in manufacturing a pharmaceutical preparation in the area ofan extremity or on a body surface, or in manufacturing a dressing material for treatment of the diabetic ulcer, where the pharmaceutical composition consists of an edible bee wax, prepared on the basis of the sesame oil extract of rhizome of Scutellaria - Huangqin (Huang Qin), rhizome of Coptis - Huanglian (Huang Lian), bark of Phellodendron- Huangbai (Huang Bai), earthworm and poppy capsule, taken in a specified ratio. The dressing material for treatment of the diabetic ulcer in the area of the extremity or on the body surface, where the said dressing material contains the pharmaceutical composition, consisting of: edible bee wax, prepared on the basis of sesame oil extract of rhizome of Scutellaria - Huangqin (Huang Qin), rhizome of Coptis - Huanglian (Huang Lian), bark of Phellodendron- Huangbai (Huang Bai), earthworm and poppy capsule, taken in a specified ratio. The first aid kit for treatment of the diabetic ulcer in the area of the extremity or on the body surface.
EFFECT: composition possesses an expressed healing activity in treatment of the diabetic ulcers.
10 cl, 20 dwg, 1 tbl, 13 ex
FIELD: food industry.
SUBSTANCE: drone larvae are separated from honeycombs. One performs pressing with subsequent adsorption of mixture of lactose and glucose into the raw product in the mixer. The prepared raw product is maintained in a sterile PET bag during 24 hours in the refrigerator. Then the raw product is arranged in a 2.0-2.5 cm layer on mylar substrates of IR-dryer screen trays; drying is performed with IR-rays during 4.0 hours at a temperature of 42-45°C in the "heating-cooling" pulse mode till production of dry crumbly clumps with moisture content equal to 10-13%. Irradiation time amounting to 5-7 sec corresponds to the "heating-cooling" pulse mode. The radiation source is represented by KGT lamps with IR radiation wavelength band equal to 1.2-2.4 mcm.
EFFECT: drone homogenate moisture content decrease, such homogenate biologically active substances preservation, reduction of microbiological contamination and decay prevention.
SUBSTANCE: ointment contains biologically active substances which are Apis mellifera in an amount of 21-23 wt %, St. John's wort oil in an amount of 12-14 wt %, propolis in an amount of 10-12 wt % and wax in an amount of 7-9 wt %, as well as Vaselin and lanolin as the ointment base.
EFFECT: invention accelerates cell regeneration processes considerably due to a synergetic action of the ingredients.
SUBSTANCE: invention relates to medicine, namely to oncology, and can be applied for antitumour immunotherapy. For this purpose, dendritic cells (DC), obtained from a patient's skin, spleen, bone marrow, thymus, lymph nodes, umbilical blood or peripheral blood (autologous DC) are introduced to the patient, with DC being introduced to the patient step-by-step. At the first stage the introduction of mature allogenic DC, loaded with a tumourspecific antigen, maturation of which is realised ex vivo, is performed. At the second stage not less than 24 hours, but not more than 120 hours after the first stage the re-introduction of allogenc DC is realised simultaneously with the introduction of immature autologous DC in situ in a tumour, with the tumour being subjected to an ablation impact preliminarily or simultaneously with DC introduction.
EFFECT: application of the claimed method makes it possible to achieve an increased immune response by the introduction of a two-component immune agent, which consists of a sensitising dose of allogenous mature cells and a therapeutic dose of immature DC, with 10% of the therapeutic DC dose being sufficient for sensitisation.
2 cl, 3 ex
SUBSTANCE: waste skin is incised on the periphery of the wound. The incised skin is prepared from the residual subcutaneous fat and milled to fragments having an area of 1-2 mm2. A dermal matrix made of dead donor's tissue is simulated after the wound shape. The dermal matrix (DM) is perforated to create traumatic discharge outflow tracts and laid on a wound bed so that to form a diastasis between perforation edges. Patient's waste skin fragments are introduced into the perforations in a staggered order. The wound is closed with a sterile gauze dressing. The other invention is allogenic mesenchymal multipotent stromal cell (MMSC) transplantation onto the wound bed in the form of the suspension by means of injections at a depth of 0.1-0.3 mm at a rate of 10 injections per 1 cm2. A volume of each injection makes 0.05-0.1 ml. The dermal matrix made of dead donor's tissue is simulated after the wound shape. That is perforated to form the traumatic discharge outflow tracts and laid on the wound bed. The wound is closed with the sterile gauze dressing. The effect is ensured by using the DM as an optimum biocompatible cell carrier and creating a required micromedium in the wound, stimulating the regeneration processes ensured by a combination with the homogenised autologous skin or allogenic MMSC injections into the wound.
EFFECT: inventions provide optimising skin and soft tissue wound healing ensured by stimulating the regeneration processes by means of stromal cells, including allo- and auto-MMSC and resident stem cells.
5 cl, 2 ex
SUBSTANCE: combined graft (CG) represents a dermal matrix (DM) prepared of a donor layer with self-specific multipotent mesenchymal stromal cells (MMSC) colonised on the surface thereof to the number of not less than 750-1500 thousand per 1 cm2. The group of inventions also refers to a method for preparing the combined graft involving bone marrow sampling from the patient, preparing a MMSC culture, applying a MMSC suspension in the concentration of not less than 1 million/ml on the DM surface at 1 million MMSC per 5 cm2 of the DM.
EFFECT: skin and soft tissue wound healing by accelerating regeneration processes.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to the pharmaceutical industry, in particular to the application of the lanocholesterol fraction of wool grease as a bioemulsifier for cosmetic anti-aging preparations. Application of the lanocholesterol fraction of wool grease as the bioemulsifier for cosmetic anti-aging preparations is obtained by alkaline hydrolysis of wool grease with a mixture of ethanol, sodium hydroxide, pyrogallol and water, or with a mixture of ethanol, sodium hydroxide, pyrogallol, toluene and water, taken in a specified ratio, with further separation and purification under certain conditions.
EFFECT: application of the described above fraction from wool grease contributes to long-lasting moistening and enhancement of the water-retaining ability of skin.
SUBSTANCE: method involves skin sampling from a dead donor in an operating room with using a skin grafting knife according to the standard procedure in compliance with aseptic and antiseptic regulations. Immediately after preparation, the biological material is placed in a sterile container with an aqueous solution of broad-spectrum antibiotic. The container is hermetically sealed keeping them sterile. The biomaterial is stored at -40°C until the donor's pathoanatomical findings have been obtained, and biological safety of the donor's tissues has been studied. The biomaterial with the proved biological safety is used for preparing the DM. Preparing the DM involves the following stages: separating the epidermis and the derma, conducting decellularisation of the derma, providing biocompatibility of the graft.
EFFECT: method enables reducing the production process to 36 hours and provides preparing the cell-free, biocompatible dermal matrix up to 1 mm thick with preserving fibre structures and direction.
2 cl, 2 tbl, 1 dwg
SUBSTANCE: invention relates to medicine and may be used for early deep burn healing. That is ensured by taking a patient's healthy tissue transplant. A cell suspension is prepared in the laboratory environment and applied together with fibrin glue (Tissucol). The intraoperative use of the invention enables reducing the length of staying on the operative table. Besides, the cell suspension applied together with fibrin glue is presented by the integral cell elements that promotes the better adhesion and the functional state continuance.
EFFECT: invention enables fixing the cell suspension reliably on the burn wound surface with using no minor chemical elements and additives when processing the cell material.
SUBSTANCE: invention relates to medicine, in particular to reconstructive surgery of peripheral nerves, and can be applied for substitution of peripheral nerve defects in case of their posttraumatic injury. Method includes intersection of neuromas and substitution of defect with autonervous insertions. Sampling of autologous fat tissue from patient is preliminarily realised by method of liposuction. Cells of stromal vascular fraction (SVF) are obtained from it after carrying out enzymatic processing. Obtained SVF cells are intraneurally transplanted into peripheral and central nerve segments, and into each insertion. Ends of nerve, as well as insertions, are covered with fibrin adhesive.
EFFECT: in conditions of clinical practice method makes it possible to restore functions of injured nerve with considerable reduction of treatment terms.
1 ex, 4 dwg
SUBSTANCE: invention refers to biotechnology and cell biology. What is presented is using stem cells of hair roots and/or prodromal keratinocyte cells for the recovery of aged, however healthy and unbroken skin for cosmetic application and skin disease prevention. Besides, what is presented is a method for aged skin recovery.
EFFECT: invention may be used in medicine and cosmetology for skin recovery.
13 cl, 1 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: group of inventions refers to biotechnology. What is described is a protein-polypeptide complex (PPC) possessing tissue specific regenerative-reparative and rejuvenating action on skin tissue, produced of extracted homogenate of nerve and skin tissues of farm hoofed embryos, a method for preparing it and a pharmaceutical composition thereof. The PPC contains tissue-specific negative faintly acid neutral proteins and peptides with molecular weight 0.5 to 200 kDa with min. 70% of total protein weight having molecular weight falling within the range of 20 to 180 kDa. The BPC is used as an active ingredient in pharmaceutical compositions applicable for preparing therapeutic and cosmetic products.
EFFECT: invention enables producing the preparations having high biological activity and applicable for treating autoimmune, cardiovascular, traumatic, toxic skin diseases, as well as used in aesthetic medicine and cosmetology.
5 cl, 14 dwg, 11 ex
SUBSTANCE: invention refers to medicine, namely surgery, and may be used for treating trophic ulcers. That is ensured by preparing an autoplasma heparin preparation. Before coating with the precipitate, the ulcer surface is covered with proper cells of a dermal surface layer.
EFFECT: invention enables treating effectively trophic ulcers in the patients suffering diabetes mellitus.
SUBSTANCE: composition 16-21ml consisting of a mixture of therapeutic preparations: Dona glucosamine sulphate 3.0 ml; ChondroGuard, or Mucosatum, or Chondrolon chondroitin sulphate - 2.0 ml; Alflutop 2.0 ml; Actovegin 4.0-5.0 ml; glucocorticosteroids Depo-Medrol 1.0-2.0 ml in the concentration of 40 mg/ml, or Diprospane 1.0-2.0 ml in the concentration of 7 mg/ml, or Metipred 1.0-2.0 ml in the concentration of 62.5 mg/ml; vitamins B1, B6, B12 Combilipen 2.0 ml, or Milgamma 2.0 ml, or Neurobion 3.0 ml; 95% or 70% ethanol 2.0-4.0 ml, is administered into a pathologically changed region. That is immediately followed by exposing the pathologically changed region and adjoining segments to a session of A shock-wave therapy in the following mode: pressure 1.2 - 4.0 bar, frequency 7-12 Hz, beat quantity 8000-14000 for 20-40 minutes. The complex procedures are performed within the course of 1-6 times every 7-10 days.
EFFECT: method enables the direct exposure on the pathologically changed region to achieve the optimum concentration of drug preparations therein with avoiding systemic prescription and preventing a risk of side effects.
3 tbl, 2 ex