Biologically active food supplement

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry, namely to a biologically active food supplement. The biologically active food supplement possessing the adaptogenic and tonic action and containing antler reindeer's whole blood, antler concentrate according to Technical Specifications (TU) 9860-010-29734071-11, honey, fruit essence, ascorbic acid and Citrosept Grapefruit taken in certain proportions.

EFFECT: formulation enables preparing the biologically active food supplement possessing the enhanced adaptogenic and tonic action.

2 cl, 8 tbl, 1 ex

 

The invention relates to the food industry and can be used in the manufacture of food supplements enriched with natural biologically active substances.

A method of producing biologically active product (pantogematogen), including the blood of deer and Sika deer at the time of cutting antlers, defibrinating blood, mixing with 96% rectified alcohol, sugar syrup, fruit essences, ascorbic acid and subsequent pasteurization of the product at 53°C for 1 h 45 min (Cm. "Antler reindeer", B. N. Ranger, N. G. Deev, tutorial, M., "Kolos", 1994, S. 52-53).

However, when defibrinating blood is lost (fibrin) 25% of formed elements (average of the blood of deer and Sika deer), and the loss of total protein is over 24%. In addition, the use of pantogematogen obtained by this method is not valid for patients with intolerance to alcohol-containing preparations, and in the treatment of diseases where alcohol form is contraindicated. In the process of pasteurization required precise temperature control, which complicates the process, and the temperature even a few degrees is associated with coagulation of blood proteins.

There is a method of production of pantogematogen, including the blood of deer and Sika deer, the stabilizer is human blood (62,0 g) and mixed with alcohol (5,5), glucose solution (32,0 g), fruit essences (0.4 g) and ascorbic acid (0.1 g), followed by pasteurization of the product by high-frequency currents for 2 hours at a temperature of 48-50°C (see RF patent №2200016, AK 35/14, publ. 2003.03.10).

However, maintaining such a minor fluctuations in temperature to ensure high quality product requires high-precision control, otherwise the consequences would be similar with the previous analogy, moreover, that this method uses whole blood and visible precipitation (coagulation) of fibrinogen occurs already at 55°C.

Izvestno psychoactive drug "Pantogematogen" including, in wt.%: defibrinating blood antler deer (56-60%), 96% ethanol - 5,2-5,6%, with the addition of sugar syrup - 35-37%, fruit essences of 0.4 - 0.6% and ascorbic acid - 0,09-0,11%. Content defibrinating blood of this product is 56-60% (see RF patent №2008008, AK 35/14, publ. 1994.02.08).

However, defibrinating involved the loss of more than 24% of the total protein of the blood, and used as a preservative ethanol contributes to the denaturation of proteins and in addition limits the scope of application of the drug, in particular, in connection with individual intolerance to the proportion of adult patients alcohol-containing preparations, and also in the treatment of children and treatment of diseases, where alcohol form is contraindicated.

Known biologically active food Supplement, obtained by mixing, wt.%: stable blood reindeer, deer and Yakut horses - 55, antler product in the form of alcoholic extract of antlers (70% solution of ethyl alcohol) - 10, a 30% aqueous solution of flower honey - 35, and represents a liquid syrupy liquid brownish-red color (see RF patent №2368250, IPC A23L 1/30, AK 35/14, AK 35/32, A23L 1/08, A23L 1/302 - prototype).

However, in the manufacture of this biologically active additives used alcoholic extract of antlers, with the extract when it goes only about 3% of dry substances panta, which leads to inefficient use of antler raw materials, and the presence of the alcohol solution in the final product limits the contingent of its consumers. In addition, the stabilization of the blood determines the introduction of environmentally friendly product chemical substances, in varying degrees, deteriorating the quality of the product. As shown by the practical use of this method of preparation of biologically active food supplements, breeding of honey contributes to the acidification of the product by 1.5-3.0% of cases, and the absence in the recipe substances that enhance the taste and prebyvajushhij the smell of blood, psychologically limits the user base, so the AK additive has a texture of a brownish-red liquid with odor and taste of animal blood. It should also be noted that the blood Yakut horses on their biological activity is at a lower level and can't be on this indicator with the blood of deer and reindeer, as the biological activity is determined not so much by the content of essential and nonessential amino acids, but to a greater extent by the presence of growth factors and uncontrolled high content (according to the patent prototype) in a mixture of blood red deer and reindeer biological activity of the product as a whole may be at a low level.

In addition, the geographical dispersion of the habitat of animals analyzed increases ten times the cost of the final product because of large transport costs for capture, storage and transportation of raw materials (the blood of deer, reindeer, and Yakut horses) to merge into a single product. All this ultimately reduces consumer properties of the product and makes it unacceptable cost for the main contingent of consumers (children, pupils, students, the elderly).

It is necessary to develop biologically active food supplements (hereinafter the product), which allows to expand the range of tools (based on antler products and honey) with adaptogenic and tonic effect.

This technical R the result is achieved by in biologically active additive to food with adaptogenic and tonic effect, including blood antler deer antler product and honey, according to the claimed invention, use whole blood antler deer, and as antler-product - concentrate on THE 9860-010-29734071-11 and fruit essence, ascorbic acid and the drug "Citrosa grapefruit" in the following ratio of components, wt.%:

Honey45,0-50,0
Concentrate0,5-1,0
Fruit essence0,2-0,4
Ascorbic acid0,08-0,1
Drug Citrosa grapefruit"0,08-0,1
Whole bloodRest

As whole blood antler deer use whole frozen blood antler deer.

Concentrate (TU 9860-010-29734071-11, RF patent №2461384) it has high biological activity, due to the presence not only of the high content of amino acids, vitamins, macro - and micronutrients and hormones, but Bo is greater degree because of the content of growth factors, with a wide range of biological effects, namely, stimulating or inhibiting mitogenes, chemotaxis and differentiation (maturation) of the cells of the body, providing a rejuvenating effect on the tissues and the whole body: re-run ofine old age or because of disease genes, thereby prolonging the life of cells, increase cell division, targeted to stimulate in them the metabolism. Growth factors stimulate the growth of the child, particularly the development of the musculoskeletal system, which is especially important during adolescence. Insulin-like growth factor (IGF) and epidermal (EGF) growth factors promote cell division and maturation of cells occurs under the action of alpha and beta transforming growth factor (TGF). In addition, transforming growth factor involved in cell proliferation and regulation of the growth of tumor cells, nerve growth factor (NGF) stimulates the regeneration of damaged neuro structures organism. The effect of growth factors should be considered in connection with other stimulants, especially with hormones, for example dehydroepiandrosterone, which also includes part of the antler concentrate. Dehydroepiandrosteron (DHEA) - the "mother hormone" of the body, is the basis on which the endocrine glands produce 27 of hormones, including testosterone and dehydrates theron. The increase of DHEA in the body can not only slow the aging process, but also to improve the condition of patients with cancer, memory loss, osteoporosis, heart diseases and so on

Cooked product does not require pasteurization, as part of the honey needed for the conservation of the number and the drug "Citrosa grapefruit". Honey has preservative (antibacterial) properties, so it is not examined for microbiological safety (see GOST 19792-2001, Honey, specifications and Uniform sanitary and epidemiological and hygienic requirements for goods subject to sanitary-epidemiological supervision (control)" chief II, section 1, p. 5.6).

Drug Citrosa grapefruit" represents an extract of bioflavonoids, grapefruit seed (33%) and vitamin C (the so-called complex of vitamin C). Drug Citrosa grapefruit" contains only herbal ingredients, no alcohol and chemical components. Pharmacological properties of the drug: antifungal (fungicidal and fungistatic), antibacterial, antiviral, widely used in medical practice, has high bioavailability, good tolerability, safety, does not destroy the internal microflora. The product produced by the firm "Chintamani international (Norway), representative office in Russia the AI LLC "Chintamani" (Moscow).

To determine the optimal content of honey is able to neutralize the smell and taste of whole (native and frozen) blood, were tested taste of products containing only honey and blood at a ratio of honey:blood- 30:70; 35:65; 40:60; 45:55; 50:50; 55:45. Studies have shown that signs of the disappearance of the smell and taste of blood were noted in the product containing honey 45-50%. Adaptogenic and tonic properties of products (with a ratio of honey:blood 50:50) from native blood and honey (prototype 1) and frozen blood and honey (prototype 2) and the product of the prototype are shown in table 1.

The studies found that the products made from whole blood antler deer and honey, even without additional enrichment antler product (concentrate) adaptogenic and tonic properties superior to the prototype 7.4-8.4 and the 11.5 and 12.4%, respectively.

In order to enhance the beneficial properties of the experimental product was introduced in the formulation of a new product concentrate on THE 9860-010-29734071-11. To determine the optimal percentage of antler concentrate product has conducted a series of experiments, the research results are reflected in table 2.

According to table 2 it is established that the product content antler concentrate 1,0-20% more than the prototype on adaptogenic action by 28.9-32.9 per cent, on the toning 21.8-27,9%. The optimal number of input antler concentrate should be considered as 0.5 to 1.0% (subject to the necessary cost of expensive antler-product - concentrate).

With the aim of eliminating the process of pasteurization (heat treatment) in the formulation of the claimed product was added drug "Citrosa grapefruit" in different doses, the results of the research are reflected in table 3. According to table 3 shows that in the product, canned drug "Nitrazepam grapefruit" in doses of 0.02 and 0.04 g, the growth of colonies detected at 6 and 10 months, respectively.

During the next month the data samples color from dark cherry moved to brown, appeared putrid smell, thick with flakes consistency with bubbles of fermentation. The product, manufactured by a prototype (without pasteurization) the same pattern was observed on the 30th day of the experiment. Product canned in doses of 0.06 to 0.12 g, for 13-15 months had a dark cherry color, fruity smell and a liquid consistency, while the shelf life of the product in all known registered technical conditions is 12 months.

Later in the course of conducting a series of experiments to establish optimal organoleptic properties for the run of the product identified the optimum content of ascorbic acid and fruit essences is 0,08-0,10 and 0,20-0,40%, because the product contains honey additionally gives a pleasant taste and partially preserves in conjunction with the drug "Nitrazepam grapefruit".

The organoleptic characteristics of the claimed product is presented in table 4.

The content of toxic elements in the claimed product complies with "the Uniform sanitary and epidemiological and hygienic requirements for goods subject to sanitary and epidemiological supervision (control), p. 8.2. (table 6).

Microbiological standards the product complies with the requirements of the Uniform sanitary and epidemiological and hygienic requirements for goods subject to sanitary and epidemiological supervision (control), p. 8.2.3 (table 7).

Definition tonic, adaptogenic properties, and increased motor activity was studied in outbred mice weighing 18-22 g

The first experimental group claimed the product was given in a dose of 0.1 ml/bird. using a probe within 20 days together with the main diet, the second experimental group (prototype) in the same way. Control animals were given saline solution and the basic diet. The research results are reflected in t the blitz 8.

During the study of the biological activity of the analyzed products found that the claimed product is superior to the analyzed samples on adaptogenic and toning action, increases locomotor activity (open field test), pain sensitivity (test "hot plate"), and so on, it should be noted that laboratory animals consumed the product of solid and whole frozen blood, significant differences when conducting biological tests had not.

Tested the claimed product in comparison with the prototype on a group of volunteers (employees of the Institute). The experiment involved 20 people (10 men and 10 women) aged from 27 to 40 years. One group (5 men and 5 women) within 15 days consumed 2 times a day for 25 ml of the claimed product, the second group (5 men and 5 women) took the product, made the prototype in the same schema.

According to the survey participants found that they were noted:

- improving performance in 10 people of the first group and 5 in the second;

- normalization of blood pressure in 9 people first group and in 4 second;

- improved sleep 10 people in the first group and in 6 second;

- improvement of health in 10 people of the first group and in 6 second.

In the end the research Institute of the experiment, Barnaul (February, 2013) according to Rospotrebnadzor was exceeded the threshold, the incidence of SARS and Influenza, the participants in the first experimental group, which consumed the claimed product, had no signs of these diseases (clinically healthy), and the participants of the second group - the 4 people mentioned high temperature, acute rhinitis, cough; 3 persons - normal temperature, chronic rhinitis and cough, 3 people the symptoms were not observed (clinically healthy).

As a result of the experiments it can be argued that the claimed product is superior to the prototype on the toning, adaptogenam, nootropic and biostimulating action.

Example.

Taking whole blood antler deer carry out the slaughter of clinically healthy animals or groups of donors using krovopoteriah systems directly connected to the tank. Whole blood not later than 3-4 hours after taking should be used for cooking or product should be frozen at a temperature of 15-20°C. Before use whole frozen blood it is thawed at a temperature of 20-25°C for 15-24 hours.

Before cooking product and mixing of all ingredients blood expanded through the installation of dispersion and gomogenizirovannogo products DG-3. Then to 450.0 g of honey mixed with 5.0 g antler is oncentrate, 2.0 g of fruit essences, 0.8 g of ascorbic acid and 0.8 g of the drug "Nitrosate grapefruit" for 20-25 minutes, after which the obtained substrate type blood - 541,4 g, continuing to stir for another 20-25 minutes. After that, the product is Packed and sealed.

Thus, the declared biologically active food Supplement allows you to expand the Arsenal of tools (based on antler products and honey) with adaptogenic and tonic effect.

1. Biologically active food Supplement with adaptogenic and tonic effect, including blood antler deer antler product and honey, characterized in that use whole blood antler deer as antler-product - concentrate on THE 9860-010-29734071-11 and fruit essence, ascorbic acid and the drug "Citrosa grapefruit" in the following ratio, wt.%:

Honey45,0-50,0
Concentrate on THE 9860-010-29734071-110,5-1,0
Fruit essence0,2-0,4
Ascorbic acid0,08-0,1
Drug Citrosa grapefruit is 0,08-0,1
Whole blood antler deerRest

2. Biologically active food Supplement under item 1, characterized in that as whole blood antler deer use whole frozen blood antler deer.



 

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