Method of recovery of oil-contaminated soils by applying of microbial-plant communities

FIELD: agriculture.

SUBSTANCE: in the method after the preliminary collection of oil from the soil surface, perennial herbal plants resistant to oil-contamination are sown in the oil-contaminated soil simultaneously with the introduction of microorganisms-oil destructors in the form of a solution of a biological product comprising microorganisms-oil destructors with mineral fertilisers.

EFFECT: method enables to increase the cleaning efficiency due to the involvement in the processes of biodegradation of oil-contamination of the wider spectrum of petroleum hydrocarbons and to accelerate the process of the soil recovery.

1 tbl, 2 ex

 

The scope to which the invention relates.

The invention relates to biotechnology, in particular to biological methods of cleaning soils from oil pollution. The method consists in the introduction of oil-contaminated soil microorganisms-oil degrading with simultaneous sowing in the soil resistant to oil pollution herbaceous perennials.

The level of technology

Intensive development of oil and gas complex leads to an increase in the number of accidents which result pollution by oil and oil products. The safest way to clean soils from oil pollution are now biological methods. To accelerate microbial biodegradation of oil are mainly two approaches: stimulation of native soil hydrocarbon-oxidizing microflora (MSD) and the introduction in oil-contaminated soil MSD and their associations, i.e., bacterial products (1. Ilarionov S. A. Ecological aspects of the remediation of oil-contaminated soils. 2004. Ekaterinburg: Ural branch of RAS, 194 C.). The introduction of the MSD with bacterial drugs is effective in cleaning oil-contaminated soils in the Northern territories as its own microbiological activity of soils is low due to the harsh climatic conditions mainly short t is plogo season 2. There are regional laws So Century, Komarov I. I., Ilyinsky centuries Introduction bacteria of the genus Rhodococcus in tundra soil contaminated with oil. Go active. Biochem. and the microbe. 1997. So 33, No. 2. S. 198-201). Currently in Russia created tens of bacterial preparations, which include from one to several dozen strains of microorganisms that are actively destroying the hydrocarbon oil and petroleum products. Typically, the microorganisms belong to the following genera: Pseudomonas, Acinetobacter, Rhodococcus, Bacillus, Candida, Aspergillus, Penicillium and some others. Among bacterial agents, for example, is well known: the drug, "Poutical", "Basicity", "MUS-1", "Universal", "Unirem", "Devoroil", "Destroil and others (3. Oborin A. A., S. A. Illarionov, Nazarov A. Century. and other oil Contaminated ecosystems. 2008. Perm: Perms. GOS.University, 388 S.).

Soil bacterial drug dramatically speeds up the process of biodegradation of oil. For more effective cleaning methods introduction microbial preparations are carried out by complex mineral and / or organic fertilizers, as well as sorbents (3).

Closest to the proposed invention prototype is a method of cleaning soil from oil pollution industrial preparation "Devoroil" (4. Pat.2023686 Russian Federation. A consortium of microorganisms used for cleaning soil and brackish ecosystems of the oil contamination / Borzenkov I. A., Melekhina I. E., Belyaev, S. S., Ivanov M. C. / B. I. 1994. No. 22). The drug is a consortium of microorganisms. It consists of bacterial and yeast cells. Bacteria represented as lipophilic (Rhodocccus sp., 367-2; Rhodococcus maris, 367-5; Rhodococcus erythropolis, 367-6) and hydrophilic (Pseudomonas stutzeri, 367-1) cultures. Yeast cells are a strain of Candida sp., 367-3. Bacteria that are part of the drug, actively oxidizes the oil n-alkanes with chain length With5-C30and aromatic compounds. The drug works in a wide range of pH (pH 5.5-9.5), temperature (5-40°C) and salinity (up to 150 g/l). According to the recommendations of the developers of a biological product the best effect is achieved with simultaneous soil mineral salts.

The disadvantages of the prototype, as well as other microbial agents, should include the following. The MSD is developing in aerobic conditions, and therefore biodegradation of contamination is mainly in the surface layers of the soil. In the presence of oil and petroleum products into the soil is the hydrophobicity of its surface, resulting in deterioration of the water-air regime of soil-atmosphere. This, in turn, negatively influences the activity of microorganisms. The activity and abundance of microorganisms-oil degrading sharply reduced in the case of formation of a crust, which retains Postup is giving moisture and air in the near-surface soil layers. The crust is formed by the evaporation of light fractions of oil and oil products and the bonding of soil particles of heavy oil residues. As a consequence, for more effective cleaning, activation of the MSD is recommended to tilling, disking soil and irrigation of contaminated sites (5. Loginov, O. N., Selishev N. N., Boiko, T. F., Galimzyanov N. F. The bioremediation. Microbiological technologies for cleaning oil-contaminated soils and industrial wastes. 2009. M.: Nauka. 112 C.), which requires additional economic costs.

The task of the invention is to develop a method of cleaning soil from oil pollution, the result of which is a higher level of treatment of the soil, accelerating their recovery, the approximation of the composition of the soil of the bitumens to natural background, economic benefit and environmental protection.

This result is achieved by the introduction of oil-contaminated soil at the same time microbial and plant communities. I.e., the recovery of oil-contaminated soil is accomplished by the introduction into it of the oil destructive microorganisms with simultaneous sowing resistant to oil pollution herbaceous perennials.

The introduction of the oil destructive microorganisms promotes rapid biodegradation of oil pollution. It is engset phytotoxicity of contaminated soils, increases germination of planted seeds and survival of seedlings. The growth of the plants, in turn, inhibits the migration of oil pollution on the surrounding properties. Education vegetative cover prevents drying out of the soil, formation of crust, which is important to preserve the MSD high microbial activity. In addition, plants in the rhizosphere (symbiotic organisms) have a positive effect on the degradation of crude oil and petroleum products. Thus, the growth of the plants on contaminated soil accelerates the biodegradation and stimulates the transformation of a wider range petroleum hydrocarbons (6. Lifshitz, S. X., Roan O N., Shashurin, M. M., Glyaznetsova Y. S., Zueva, I. N., The kershengoltsa B. M. Transformation of oil pollution and the formation of adaptive plant responses in the model experiment with permafrost soils of Yakutia /Chemistry for sustainable development. 2011. No. 19. S. 169-178). As a consequence, a decrease in the proportion of 2 - and 3-methylalkanes and a significant decrease in the content of the relic 12 - and 13-methylalkanes, not typical for organic matter modern precipitation; the transformation of isoprenoids; an additional maximum in the high molecular weight region of na27,29,31characteristic distribution of n-alkanes modern precipitation; increased value of the ratio of odd-numbered n-ALK is new to even compared with the value thereof in oils, close to 1.

Plants growing on contaminated soil, adapting to the toxic environment, use the Arsenal all possible protection mechanisms: increasing the activity of antioxidant systems and reparation of DNA to activate apoptosis and SOS reparations, and of the products of biodegradation of oil pollutions are used by plants as a nutrient medium (7. Lifshits, S. Kh., Chalaya O. N., Glaznetsova Yu.S., Zueva I. N. The effect of oil contamination on the adaptive potential of plants in criolite zones /AAAPG-2012. Abstract of "The 8thIntern. Conf. on Petroleum Geocemistry and Exploration in the Afro-Asian Region". Hangzhou, China. November 2-4, 2012. P. 91).

At high contamination of the plants are killed, but those seedlings that were able to adapt and survive to the next year give numerous shoots. Consequently, it is advisable to sow perennials, because they established adaptive capacity is not lost and is manifested in the following offspring. This contributes to a more rapid restoration of disturbed lands.

Thus, biotechnological result achieved when the task is to achieve a synergistic effect, when each of the performed activities (introduction of the oil destructive microorganisms and sowing resistant to oil pollution perennial herbaceous plants) enhances the positive effect of the other ways is helping the implementation of positive feedbacks. I.e. the implementation of the proposed method of remediation of oil-contaminated soils is aimed at vsokoaktive work included microbial and plant components, which increases the rate of biodegradation of pollution, helps to ensure the stability of the processes involved in the transformation processes over a wide range of hydrocarbons and the approximation of the composition of the soil of the bitumens to natural background.

The proposed method of cleaning soil from oil pollution is environmentally friendly because its implementation uses biological systems (MSD and plants), the functioning of which accelerates the biodegradation of oil pollution.

The proposed method can be used to clean soils from oil spills varying degrees after the preliminary Assembly with the soil surface excess oil. The effectiveness of the proposed method was evaluated by the degree of biodegradation of oil pollution (the ratio of the number biodegradeable oil to the source of pollution, in percent) and for the approximation of the composition of the soil of bitumen to the natural background, which was evaluated on a range of geochemical indicators shown in the table. For comparison, the table shows the geochemical indicators for clean soil (natural background).

The working solution of the drug "De oroel" with mineral fertilizers for treatment of oil-contaminated soil was prepared according to the instructions (8. Instructions for use of the biological product "Devoroil". Approved by the state Committee for sanitary and epidemiological supervision of the President of the Russian Federation, No. 01-13/1102-1129.08.1995). Calculation of consumption seeds for sowing was determined according to the recommendations (9. Gendrin A. G., Nadachowska, A., Sidorenko I.e. and other Environmental support oil and gas fields. Issue 1. Novosibirsk. 2005. 112 C. (Ser. Ecology. Vol.78)).

Example 1. In oil-contaminated soil with the level of pollution with oil, 4.8 g/kg of soil was made biopreparation "Devoroil in a dose of 5 kg/ha and at the same time are sown the seeds of Timothy grass meadow (Phleum prantense at the rate of 35 kg/ha Timothy meadow promotes dermoabrasion and accumulation of humus. Samples of soils were sampled 3 months after the beginning of the restoration works. The results of the experiment are shown in table. The degree of biodegradation of oil pollution for 3 months was 87%. In the composition of the soil bitumen marked changes in the direction of restoring the natural background. This is evidenced by changes in geochemical parameters, such as: reduction of the hydrocarbon content and the increase resins; in group composition alkanovykh hydrocarbons significantly decreased the content of 2 - and 3-methylalkanes and 12-and 13-methylalkanes, as well as the relatively low molecular weight n-alkanes, resulting ratio Σ.to.-na20Σ 21-K. K. was equal to 0.43 in comparison with 1.58 for contaminated soil; a maximum of n-alkanes has moved into high molecular weight region NS,29,31; ratio LF/h increased from 1.13 to 2,81.

Example 2. In oil-contaminated soil with the level of oil pollution 12.3 g/kg of soil was made biopreparation "Devoroil" in a dose of 10 kg/ha and at the same time are sown the seeds of red clover (Trifolium prantense at the rate of 30 kg/ha red Clover was selected with the aim of enriching soil recultivating site nodule bacteria that live in the rhizosphere, fixing atmospheric nitrogen, necessary for the development of the MSD, which should also affect the intensification of processes of destruction of hydrocarbons and fertility restoration of disturbed lands. Samples of soils were sampled 3 months after the beginning of the restoration works. The results of the experiment are shown in table. As you can see, after rehabilitation there is a high level of treatment of the soil, the degree of biodegradation was 81%. In the composition of the soil bitumen tends to approach to the natural background. These are: the decrease in the composition of the soil bitumen hydrocarbons and increase resins. In the group composition alkanovykh hydrocarbons marked redistribution in the direction of decreasing the content of 2 - and 3-methylalkanes and 12 - and 13-methylalkanes. Mean is Ino decreased the number of relatively low molecular weight n-alkanes, therefore the ratio Σ.to.-na2021-K. K. became equals 0.58 compared from 1.62 to contaminated soil. A maximum of n-alkanes has moved into high molecular weight region of NA27. Began to increase the ratio LF/h

The method of biological treatment of soils from oil pollution with the introduction of the oil destructive microorganisms, characterized in that after the preliminary Assembly with the soil surface oil in oil-contaminated soil simultaneously with the introduction of the oil destructive microorganisms in the form of a solution of a biological product containing microorganisms-oil degrading with mineral fertilizers, seeded resistant to oil pollution perennial herbaceous plants.



 

Same patents:

FIELD: biotechnology.

SUBSTANCE: proposed strain Pseudomonas migulae is deposited in the Russian National Collection of Microorganisms of the Institute of Biochemistry and Physiology of Microorganisms under the number of VKM B-2761D. The strain is isolated from samples of bottom soil contaminated with oil of freshwater lake of Usinsky district of the Komi Republic in the zone of chronic oil pollution.

EFFECT: strain has high oil-oxidising activity against naphthenic hydrocarbons, is capable of reducing the concentration of hydrocarbons dissolved in water and accelerates the destruction of oil in soils and bottom sediments of water reservoirs.

3 tbl, 3 ex

FIELD: biotechnology.

SUBSTANCE: bacterial strain Rhodococcus rhodochrous is deposited in the Russian Collection of Microorganisms of the Institute of Biochemistry and Physiology of Microorganisms RAS under the registration number RCM Ac-2018 D. The destruction with this strain within 7 days of crude oil is up 100%, oil sludge - up to 98%, black oil - up to 96%.

EFFECT: strain has high destructive activity against petroleum hydrocarbons included in oil sludge, as well as against crude oil and black oil.

1 dwg, 1 tbl, 3 ex

FIELD: biotechnology.

SUBSTANCE: strain Gordona sp. is deposited in the Russian National Collection of Microorganisms of the Institute of Biochemistry and Physiology of Microorganisms RAS under the registration number RNCM Ac-2044 D. The destruction with this strain on the 7th day of crude oil is up to 100%, oil sludge - up to 99%, black oil - up to 97%.

EFFECT: strain has high destructive activity against petroleum hydrocarbons included in oil sludge, as well as against crude oil and black oil.

1 dwg, 1 tbl, 3 ex

FIELD: chemistry.

SUBSTANCE: invention relates to microbiology. Disclosed is a Pseudomonas stutzeri VKPM V-11230 bacteria strain which is a decomposer of aliphatic and aromatic petroleum hydrocarbons and a plant growth stimulant in association with plants. Disclosed is use of said strain to decompose hydrocarbon contaminants in oil-contaminated soil and petrochemical sludge, for in situ phytoremediation of petrochemical sludge and oil-contaminated soil, including in conditions with high content of hydrocarbons and accompanying contamination with heavy metals.

EFFECT: strain is used to produce indoleacetic acid in association with plants for stimulating plant growth and for fixing nitrogen from the atmosphere in phytoremediation of media with nitrogen deficit.

8 cl, 1 tbl

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology, particularly to microbiological methods of cleaning the environment. The method includes preparing a mixture of liquid bacterial cultures in the form of Pseudomonas fluorescens VKG RCAM00538 strains with a titre of 10-13, KOA-4 Pseudomonas fluorescens ND-610 VNIISKHM with a titre of 10-10, Azotobacter chroococcum AIN RCAM00539 with a titre of 10-12 in ratio of 3:1.5:0.5-3:2:1, respectively. The method includes treating wastes from beneficiation of brown coal by adding the obtained liquid mixture of strains followed by mixing and drying the prepared mixture in not less than 2.5 days at room temperature, wherein the wastes from beneficiation of brown coal and the prepared mixture of strains are taken in the ratio of 40-50:50-60 wt %, respectively.

EFFECT: invention increases content of humic, carboxylic and amino acids and polysaccharides in the biopreparation at minimal costs and duration of production.

7 tbl, 7 ex

FIELD: ecology.

SUBSTANCE: soil contaminated with oil products is detoxified by adding a natural sorbent with a biopreparation until a given concentration of the contaminant in the soil is achieved. The sorbent used is glauconite and the biopreparation used is polyculture. Before adding the sorbent with the biopreparation, concentration of the contaminant is measured, the volume of sorbent is determined and a shielding intermediate layer of biohumus is placed at the boundary where the contaminant penetrates the soil in the lower part of the contaminated layer. Concentration of the contaminant is determined layer-by-layer. The number of layers of contaminated soil with concentration of the contaminant in the layer differing from each other by at least double is not less than two. The contaminated soil is moistened after distributing the volume of sorbent with the biopreparation, effective microorganisms and fermented compost on the surface of the contaminated soil while simultaneously mixing. A water-swelling polymer is also added to the contaminated soil. Temperature conditions for activating biological processes are maintained by adding organic materials to the soil, wherein the volume of said organic materials in the shielding intermediate layer should be at least double that in the contaminated soil.

EFFECT: high efficiency and faster detoxification.

1 tbl

FIELD: chemistry.

SUBSTANCE: group of inventions relates to biotechnology. Claimed is a polypeptide, obtained from strain SA-01 Thermus scotoductus, responsible for the reduction of uranium (VI) in a uranium (VI) source to uranium (IV), where the polypeptide includes an amino acid sequence SEQ ID NO:1. Also claimed are a separated molecule of nucleic acid, coding the polypeptide and including a nucleotide sequence SEQ ID NO:2, an expression vector, including the said molecules of nucleic acid, a host cell, including the said vector. Also claimed are a method of obtaining the said polypeptide and its application in the reduction of uranium (VI) in the source of uranium (VI) to uranium (IV).

EFFECT: group of inventions provides a high degree of reduction of uranium (VI) to uranium (IV).

9 cl, 10 dwg

FIELD: chemistry.

SUBSTANCE: claimed is a method of water and permafrost soil purification from oil and oil products. The method includes the application of a bacterial suspension based on cells of nonpathogenic strain of the bacteria Pseudomonas panipatensis VKPM V-10593 with titre 1·109 microbial cells/cm3.

EFFECT: invention ensures a high degree of soil and water purification from oil and oil products in a wide range of temperatures.

3 tbl, 4 ex

FIELD: biotechnology.

SUBSTANCE: bacterial strain Bacillus vallismortis RNCIM B-11017 is grown and a suspension is made of it, which is applied in cryogenic soil and the water environment. It is exposed under the specified parameters from 7 to 60 days and the quantitative content of crude oil and petroleum products in the cryogenic soil and water environment is determined.

EFFECT: invention enables to reduce the time of denaturation of crude oil and petroleum products and to reduce their concentration in the cryogenic soil and water environment.

3 tbl, 4 ex

FIELD: biotechnology.

SUBSTANCE: bacterial strain Exiguobacterium mexicanum RNCIM B-11011 is proposed, having the ability to dispose quickly of oil, diesel fuel, motor oil, gas condensate.

EFFECT: strain can be used to clean soil and water reservoirs contaminated by crude oil and petroleum products in a wide temperature range.

3 tbl, 5 ex

FIELD: agriculture.

SUBSTANCE: method comprises undercutting of contaminated topsoil, throwing to the bottom of the furrow and piling it with an uncontaminated layer. The contaminated soil layer is undercut by the soil tillage body of a scraper type and moved aside to form a ridge, and the uncontaminated soil fertile layer is opened. The open uncontaminated soil layer is undercut by the combined working body and lifted to the conveyor without the layer overturning. The soil layer is transported first up, then down, leaving a passage for the contaminated layer undercut by the lateral working body of a scraper type under the ridge of the contaminated layer formed by the first working body, and together they are moved to the bottom of the furrow. Then the contaminated soil layer is compacted and closed with the uncontaminated layer passing down from the conveyor. After that the soil is levelled and compacted to the operating width of the device. The device comprises a rectangular frame, support wheels, a mechanism of attachment to the tractor and the working bodies. The first and the lateral working bodies are made of a scraper type. The device also has a combined working body composed of two arranged towards each other rigidly interconnected working bodies of the mouldboard type plough. The total mouldboard part of the combined working body is made truncated in the form of a trough. Behind the combined working body there is a conveyor with ascending, horizontal and descending parts, having a drive of PTO of the traction unit through the power shaft, a reducer and a chain drive. Behind the conveyor a pusher is mounted for levelling the surface of the field after the passage of the device and a packing roller, which is a support one in the transfer of the device to the transport position by the hydraulic cylinder attached to the median longitudinal bar of the frame.

EFFECT: improving the quality of treatment of soil contaminated with radionuclides and heavy metals by avoiding mixing of the contaminated and uncontaminated soil layers in the process of their moving.

7 cl, 2 dwg

FIELD: agriculture.

SUBSTANCE: group of inventions relates to the field of agriculture. The method comprises pasturing by animals, destroying of not eaten, harmful and poisonous plants, levelling animal excrement, and removal of uneaten residues by harrows and rakes, fertiliser application, as well as creation of tree and shrub shelterbelts. At that on the area intended for hayfields and pastures area after pasturing by animals the fertilisers are initially applied, and then the uneaten plants are destroyed, tilting them and applying herbicide by method of smearing it on their biomass, including stems and lower surface of leaves. Harrowing and treatment the grass with rake is carried out after the start of regrowth of plants eaten by animals. The device comprises a reservoir for the herbicide, as well as a pump for feeding it to the tubular perforated rods with means for application of the herbicide to the plants. The perforated rods are mounted behind each other in parallel with the ability of horizontal and vertical displacement, and the device for application of the herbicide to the plants is made in the form of capillary cloth located between the pressure plates and attached on the perforated parts of the said rods which are connected and framed with the cloth and placed in the tubular casings with longitudinal slots through which the cloth is passed, compressed by the pressure plates fixed on the edges of the slots. And the perforated tubular rods are communicated with each other by the hose, at that the inlet end of the front tubular rod is connected to the pump and the outlet end of the rear rod through the drainage tube with the valve - with a reservoir for the herbicide to form a flow regulated system. Furthermore, the perforated tubular rods and their casings are attached to the frame, mounted on the propellers, with freedom of vertical and horizontal displacement.

EFFECT: inventions enable to simplify the technology of creation of forage land on fallow lands with simultaneous formation of tree-shrub belts.

6 cl, 3 dwg, 2 ex

FIELD: chemistry.

SUBSTANCE: invention relates to environmental conservation technologies, cutting-edge agricultural technologies and chemistry of organosilicon compounds and can be used to restore the structure of disturbed soils by stabilising water-stable aggregate. In particular, the present invention employs water-soluble humic silanol derivatives that are modified in a special way to endow them with the capacity to be irreversibly sorbed on surfaces of mineral particles and other hydroxyl-containing carriers. Humic derivatives are added to the degraded soil in form of aqueous solutions.

EFFECT: method for use in agricultural technologies includes using humic derivatives as structure-forming agents (soil ameliorants) by via attachment thereof to the surface of soil aggregates in order to endow the latter with water-stable properties.

8 cl, 11 dwg, 8 tbl

FIELD: agriculture.

SUBSTANCE: invention relates to the field of environmental protection and can be used in the rectification of the consequences of the oil spill on the soil. The method consists in mixing the oil-contaminated soil, spread over the surface of freshly ploughed uncontaminated field with the thickness layer from 1/10 to 1/6 of the ploughing depth, to the full depth of ploughing using the tiller several times.

EFFECT: invention enables to restore oil-contaminated soil without application of additional neutralising foreign substances and reagents.

FIELD: oil and gas industry.

SUBSTANCE: for biorecycling of oil sludge and oil production and processing waste by means of soil populating with natural black-soil-forming organisms a mixture is prepared with an initial composition of 26-30% of oil sludge, 26-30% of liquid oil sludge and the remaining part of organic domestic waste and/or saw dust, the mixture is infused within 20-24 hours at a temperature of 20-25°C for further populating with earthworms of the first generation, the mixture is withhold within 17-30 days till the earthworms of the second and third generations appear for the purpose of the sludge-contaminated soil treatment. The earthworms of the second and third generations grown in a hothouse conditions by adaption of the earthworms of the first generation to the above mixture are introduced to the contaminated soil. At that introduction of the earthworms of the second and third generations is made at a volume ratio of soil and the mixture equal to 10-15:1.

EFFECT: improving efficiency of the contaminated soils treatment.

4 cl

FIELD: chemistry.

SUBSTANCE: soil-sludge mixture is obtained by thermal treatment and contains a mixture of components obtained by processing wastes in the UZG-1MGZH apparatus for treating oil-contaminated soil in amount of 30-40 wt %, peat 20-35 wt % and sand 20-50 wt %, wherein the mixture of components contains, wt %: vanadium oxide 32.5; carbon 31.4; aluminium oxide 7.5; silicon oxide 7.5; nitrogen oxide 6.8; iron oxide 5.3; hydrogen 4.4; magnesium oxide 1.5; manganese oxide 0.8; lead oxide 0.4; chromium oxide 0.4; zinc oxide 0.4; nitrogen 0.4 and related impurities 0.7.

EFFECT: improving environmental conditions, restoring fertility of oil-contaminated and disturbed lands, higher fertility as a result of enrichment of contaminated soil with oxygen and mineral fertilisers during treatment and reducing oil-contaminated areas.

3 cl, 3 tbl

FIELD: chemistry.

SUBSTANCE: soil-sludge mixture for reclamation of oil-contaminated and disturbed lands contains a mixture obtained by treating a composition consisting of oil sludge, peat, sand and water, with the following ratio of components, wt %: vanadium oxide 32.5; carbon 31.4; aluminium oxide 7.5; silicon oxide 7.5; nitrogen oxide 6.8; iron oxide 5.3; hydrogen 4.4; magnesium oxide 1.5; manganese oxide 0.8; lead oxide 0.4; chromium oxide 0.4; zinc oxide 0.4; nitrogen 0.4; related impurities 0.7. Components of the composition of oil sludge, peat, sand and water are in the following ratio, wt %: oil sludge 30-40%; peat 30-35%; sand 30-35%; water - the balance. The related impurities include a proppant which contains ceramic spheres, a cut of the oil-contaminated soil layer containing crushed stone, and fine wastes of ferrous and nonferrous metals. Treatment involves mixing the composition of oil sludge, peat, sand and water in a hopper of an UZN-1MGZH apparatus.

EFFECT: improving environmental conditions, restoring fertility of oil-contaminated and disturbed lands, higher fertility as a result of enrichment of contaminated soil with oxygen and mineral fertilisers during treatment and reducing oil-contaminated areas.

4 cl, 1 tbl

FIELD: chemistry.

SUBSTANCE: invention relates to environmental protection. The composition for cleaning soil contaminated with oil and motor fuel comprises peat filler, nitrogen and phosphorus fertiliser, used in ratio N:P2O5=1-10:1, zeolite-clay rock modified with a cationic surfactant, and cation-exchange minerals, with the following content of components, wt %: low- or high-moor peat 44.79-93.989; nitrogen fertiliser, N 0.01-0.15; phosphorus fertiliser, P2O5 0.001-0.06; zeolite-clay rock, modified with a cationic surfactant 1-15; cation-exchange minerals 5-40.

EFFECT: invention provides localisation and biodegradation of a hydrocarbon contaminant with high efficiency and prolonged effect.

6 cl, 4 ex

FIELD: process engineering.

SUBSTANCE: invention relates to reclamation of soils contaminated with oil products, decontamination of soils, liquidation of oil spillage, etc. Proposed device comprises intake bin, mixer, crusher, cleaned soil handling device, screw conveyor and vibrator. Crusher is arranged above intake bin and communicated via screw conveyor with mixer. Mixer represents and encased horizontal screw mixer with pelletised sorbent dispenser arranged at its top part. Mixer outlet is connected via screw conveyor with drier bin. Drier is composed by housing accommodating belt conveyors to carry the material along the drier and to pour it down onto belt conveyor. Conveyor top runs contact with vibrators mounted there under. Said vibrators are composed by roller eccentrics with individual or group drive. Burners to combust sorbent with oil products are arranged in drier housing above said conveyors. Drier top section accommodates the off-gas collection chamber communicated with cyclone and absorber via smoke sucker. Cleaned soil handling device is mounted at outlet of the last conveyor and composed by discharge channel connected with opening in drier housing sidewall to be closed by shutter.

EFFECT: higher intensity of oil removal irrespective of seasons.

1 dwg

FIELD: biotechnology.

SUBSTANCE: for bioremediation of cadmium-contaminated soils first before sowing peas, the inoculum is prepared from symbiotic microorganisms resistant to cadmium, consisting of soil-root mixture containing the strain of arbuscular mycorrhiza fungus Glomus sp.Fo 1 deposited in the collection of state scientific institution All-Russian Research Institute for Agricultural Microbiology of Russian Agricultural Academy under the number RCAM00630, in the amount of 0.5-1.0 g of a mixture per 1 pea seed; the aqueous suspension of nodule bacteria containing the strain Rhizobium leguminosarum bv. viciae deposited in the collection of state scientific institution All-Russian Research Institute for Agricultural Microbiology of Russian Agricultural Academy under the number RCAM1066, containing 106-107 cells of bacteria per 1 ml of the suspension, in the amount of 0.1-0.5 ml suspension per 1 pea seed, and the aqueous suspension of associative bacteria containing the strain Variovorax paradoxus 5C-2, deposited in the collection of state scientific institution All-Russian Research Institute for Agricultural Microbiology of Russian Agricultural Academy under the number RCAM00049, containing 106-107 cells of bacteria per 1 ml of the suspension in an amount of 0.1-0.5 ml of the suspension per 1 pea seed. Then in the cadmium-contaminated soil the seeds of genetically modified peas SGECdt are sown, which have higher resistance and accumulation of cadmium in an amount of 20-60 seeds per 1 m2, combining them during sowing with an inoculum following the specified standards, and the plants are grown to full maturity of beans, then the peas are mowed and removed from the soil surface.

EFFECT: accelerated recovery process of healthy biocenosis of soil.

2 tbl

FIELD: soil restoration from formation fluid contaminants in oil recovery and transportation regions.

SUBSTANCE: claimed composition contains (mass %): chemical ameliorant 3.5-5.7; organic fertilizer 15.7-29.0; adsorbent 67.5-78.6. Method for sectioning soil treatment includes application of composition onto soil layer with 30 cm of depth. Then treated layer is shifted out of spot boundary. Opened surface is covered with composition of present invention. Then mole drainage of 60-65 cm in depth is made in soil by using mole plow or chisel plow, followed by plowing of 58-51 cm in depth and returning of sheared soil into spot.

EFFECT: effective soil restoration from formation fluid contaminants.

5 cl, 2 dwg, 1 tbl

Up!