Method for detecting cognitive disorders in white rats
SUBSTANCE: single-step testing of white rats is carried out in an open field facility. The total length of locomotion, sniffing, and sitting actions as well as the number of upright postures and freezing acts are recorded. Prognostic coefficients F1 and F2 are determined by formulas and compared. If F2 is more than F1, cognitive disorders are stated in experimental animals, and if F1 is more than F2, the above are stated to be absent. That is followed by calculating a prognostic index (PI), which is used to determine an intensity of the cognitive disorders: low, moderate and high.
EFFECT: method enables detecting the presence of the cognitive disorders in the rats by using the values of motor and investigatory activity and anxiety.
2 cl, 2 tbl, 4 ex
The invention relates to medicine, specifically to experimental medicine, and can be used to study the cognitive performance of white rats in norm and at a pathology of various origins.
Known methods for performance evaluation of cognitive functions by testing experimental animals in tests "radial maze, maze Morris", "T-maze" [2, 5]. The main characteristic of these methods is the long period of training animals, an average of from 8 to 15 days and more.
The closest way to study the cognitive animal performance (prototype) is a method of studying the peculiarities of higher nervous activity, consisting in the development of conventional drinking reflex in the T-shaped maze . However, this method is rather time-consuming and of a conditioned reflex in animals requires a long period of time (10 days). When planning experimental studies arises the need for a preliminary assessment of the cognitive capacities of animals, which will allow you to expand or narrow the set of experimental methods of research in the future.
The problem solved by this invention is to develop a way of identifying cognitive impairment in white rats, allowing to reduce the complexity and time is of atrati on the experiment.
This object is achieved in that conduct testing of white rats in open field: for 3 minutes record the following indicators: total duration acts "locomotion", "obnyukhivanie", "sitting"; the number of vertical columns; the number of acts "freezing". Expect prognostic factors according to the formula:
where F1and F2- prognostic factors, a1- the total duration of the act of "obnyukhivanie" (C), a2- the total duration of the act of "sitting" (C), a3- the total duration of the act of "locomotion" (C), a4- the number of vertical racks, a5the number of acts "freezing".
If F1more F2make a conclusion about the absence of cognitive impairment in experimental animals, and when F2more F1- make a conclusion about the presence of cognitive impairment in experimental animals and determine their severity using a prognostic index (PI), which is calculated by the formula:
where e is the base of natural logarithm (2,72...).
If PI is more than 0.5 and less of 0.64, the severity of cognitive impairment is defined as low, if PI is more of 0.65 and less 0,84 medium, when PI is more of 0.85 as high.
New in the present invention is that the test results in the "open field" in terms of motor and orienting-exploratory activity and anxiety it is possible to detect the presence of these individuals impaired cognitive functions such as learning ability and spatial orientation, as well as violation of the processes of working memory. Moreover, when calculating the prognostic index is determined by the severity of cognitive impairment in animals.
The authors are not found in the analyzed literature way of identifying cognitive impairment in white rats on the performance of their behavior in the open field.
Comparison of the invention with existing methods of assessment of cognitive abilities of white rats showed that for the first time proposed to establish the presence and assess the degree of cognitive impairment in rats by recording their behavior in open-field test.
Thus, the claimed invention meets the criteria of the invention of "Novelty" and "Inventive step", as it is the main way is not necessary for the expert from the prior art. The present invention meets the criterion of "Industrial applicability" because it can be used in experimental medicine, physiology, pathological physiology, pharmacology to identify the cognitive animal performance and when planning the design of experimental research.
In numerous studies the method of "open field" is used to assess the condition of the Central nervous system of animals [1, 4]. Recorded in this test the performance behavior characterized by orienting-investigative behavior (obnyukhivanie), motor activity (locomotion, sitting) and anxiety (freezing). Orienting-investigative activity, as a manifestation of the higher nervous activity, is one of the most important types of behavior by which animals acquire knowledge about the environment and demonstrate reasoning. In pharmacology increasing locomotor and orienting-exploratory activity of the animals of the experimental groups when compared to control indicates the presence of a possible neuroprotective effect of the studied compounds [4, 5]. At the same time to assess these metrics cognitive functions of animals, such as working memory, learning ability and spatial orientation, it is necessary to conduct to olitely studies using standard techniques, such as a conditional reflex active and passive avoidance (passive avoidance reaction and AVOIDANCE), T-maze, radial maze, water maze Morris, etc.
The experiment was carried out on outbred rats in the amount of 140 individuals weighing 180-240 g, obtained from own nursery Angarsk branch of the fgbi "wsnt EB" SB RAMS. Testing white rats was performed using a setup open field, representing a circular arena with a diameter of 97 cm, height of walls 42 cm, diameter of the holes in the floor - 2, see the Observation was made within 3 minutes with the registration of the following indicators: total duration acts "locomotion", "obnyukhivanie", "sitting"; the number of vertical columns; the number of acts "freezing".
The obtained results were processed using the SPT Statistica 6.0. (criteria Mann-Whitney, discriminant analysis, persons No. AXXR004E642326FA).
Table 1 allows us to draw conclusions regarding the statistical significance of each of the dependent variables included in the model is constructed. In this case, all the studied variables have a highly significant influence on the dependent variable (p<0,05). The significance level for the model was 0.0001.
|Indicators informative paragraph is Isakov, included in the linear discriminant functions|
|A variable in the model||Lambda Wilks||F||p-level|
|The total duration of Abdugani"||0,42||44,64||0,00001|
|The total duration of the "sitting"||0,41||41,37||0,0001|
|The total duration of "locomotion"||0,27||12,01||0,001|
|The number of vertical racks||0,26||10,18||0,0026|
|The number of act "rising"||0,24||of 7.36||0,0091|
To confirm the cognitive status of white rats conducted an assessment of working memory, learning ability, spatial orientation and navigation learning when tested in the radial maze and a Morris water maze.
Table 2 presents the results of the test animals in the "open field", "radial is the labyrinth, a Morris water maze (data are presented as medians and interquartile scale).
|Performance testing of animals in the "open field", "radial maze, water maze Morris, Me (LQ; UQ)|
|Group # 1 acute prenatal hypoxia"||Group # 2 "chronic prenatal hypoxia"||Group # 3 "toluene"||Group # 4 "control"|
|The total duration of obnyukhivanie"||46 (38; 51,5)||31 (25; 37)*||32,5 (28; 39)||52 (42; 71)|
|The number of vertical racks||4 (2; 6)||2,5 (1; 4)*||5 (4; 6)||4 (3; 6)|
|The total duration of locomotion"||64 (21; 79)*||77 (62; 95)||97 (88; 106)*||73 (61; 92)|
|The total duration of the "sitting"||46 (23; 85)*||43 (23; 56)*||5 (4; 6)||16 (7,5; 26)|
|The number of acts "rising"||2 (1; 4)*||1 (1; 2)*||1 (0; 2)||1 (0; 1)|
|The number of working memory errors (last test session)||0 (0; 0,5)||0,5 (0; 1)*||0 (0; 0,5)||0 (0; 0)|
|The number of consecutively visited hoses (last test session)||3,5 (3; 4)||2 (3; 3)*||3 (3; 4)||4 (3; 4)|
|Water maze Morris|
|The latent period of search for a hidden platform||68,5 (40,5; 78,0)*||59,5 (46,3; 7,8)*||51,3 (37-73,5)*||42,7 (31,8; 61,5)|
|Note: * - the differences are statistically significant compared to control at p<0.05; number of animals in each group - 35|
White rat exposed to chronic prenatal hypoxia. During examination in open field test revealed numeric values:
a1- the total duration of the act of "obnyukhivanie" - 32,5,
a2- the total duration of the act of "sitting" - 23,
a3- the total duration of the act of "locomotion" - 109.7 from,
a4- the number of vertical columns is 0,
a5the number of acts "rising" - 1.
The calculated prognostic factors F1and F2by the formulas:
Conclusion: the presence of a given individual cognitive impairment high degree. The testing results of the water and the radial maze showed that this animal indicators of cognitive abilities significantly reduced compared with the control group. Thus, the latent period locate a hidden platform in the water maze in a given individual was 61,9 C, considerably higher than the average of the control group (427 (31,8; 61,5) C. When learning in the radial maze, the number of working memory errors (2 errors) in the last training session, this individual was higher than control group 0 (0; 0). The number of consecutively visited backed by arms of the maze in a given individual (2) was significantly lower than the control group 4 (3; 4) (table.2).
The white rat, subjected to inhalation exposure to toluene. During examination in open field test revealed numeric values:
a1- the total duration of the act of "obnyukhivanie" - 55,
a2- the total duration of the act of "sitting" - 19,5 with,
a3- the total duration of the act of "locomotion" - 79,7 with,
a4- the number of vertical racks - 1,
a5the number of acts "rising" - 3.
The calculated prognostic factors F1 and F2 by the formulas:
Conclusion: the presence of a given individual cognitive impairment low severity. The examination of water and radial mazes confirmed the presence of cognitive impairment from this animal. This individual was observed the increase of the latent period locate a hidden platform in a water maze, which amounted to 57.2 from and far above the average indicators of the group I control (42,7 (31,8; 61,5) C. When learning in the radial maze obvious violations of working memory have been identified (table.2).
The white rat, subjected to acute prenatal hypoxia. During examination in open field test revealed numeric values:
a1- the total duration of the act of "obnyukhivanie", 57 p,
a2- the total duration of the act of "sitting" - 23,
a3- the total duration of the act of "locomotion" - 85,
a4- the number of vertical racks - 1,
a5the number of acts "rising" - 2.
The calculated prognostic factors F1and F2by the formulas:
Conclusion: the presence of a given individual cognitive impairment moderate severity. When tested in water and the radial maze were confirmed by the results obtained. This animal was observed the increase of the latent period locate a hidden platform in the water maze (69,1) and the number of working memory errors when learning in the radial maze (3) compared with the average performance of the control group (table.2).
White rat from the control group. During examination in open field test revealed numeric values:
a1- the sum of the Naya duration of the act of "obnyukhivanie" - 59,2 with,
a2- the total duration of the act of "sitting" - 27,9 with,
a3- the total duration of the act of "locomotion" - 73.2 C,
a4- the number of vertical columns is 0,
a5the number of acts "rising" - 1.
The calculated prognostic factors F1 and F2 by the formulas:
Conclusion: there are no cognitive impairment in a given individual. Testing in water and radial mazes confirmed the results obtained. Violations ability to navigation to learning and change processes spatial and working memory were observed. Indicators of cognitive abilities of the species - the latent period locate a hidden platform (35), the number of working memory errors (0) and the number of consecutively visited reinforced sleeve radial maze in the last test session (4) were within the average of the control group (table.2).
Thus, the proposed method is less time-consuming and requires less time to experiment.
1. Training in a Morris water maze females and male rats exposed to hypoxia in different periods of prenatal development period / O. I. Wateva [and other] // Journal of evolutionary biokhimiia physiology. - 2005. - T. 41, No. 6. - S. 530-533.
2. Samostroev M. A. Experimental models of behavior / M. A. Samostroev, D. L. Warm, And. And. Tyurenkov // science. - 2009. No. 2. - S. 140-152.
3. Pershina O. C., Suslov N. And., Litvinenko C. I., Popov, Etc., the Effects of an extract from the aerial parts of Scutellaria baicalensis Georgi on the production and reproduction of conventional drinking reflex in intact animals and animals with alcohol intoxication // Plant resources. - 2000. - Vol.2. - S. 120-124.
4. Ivanov D., the Relationship between the metabolism of collagen and behavior of rats in the open-field test / A., Ivanov, V., Podkovkin // Successes of modern natural science. - 2010. No. 5. - S. 16-20.
5. Podkovkin Century, the Influence of short-term isolation on the behavior of rats in the open-field test / Century, Podkovkin, D., Ivanov / Successes of modern natural science. - 2009. No. 6. - S. 12-16.
1. How to detect cognitive impairment in white rats, which consists in studying the behavior of animals, wherein the animals are examined in open-field test, within three minutes, record the total duration acts "Obrucheva", "sitting", "locomotion", the number of act "rising" and the number of vertical pillars, determine prognostic factors F1and F2formula:
where F1and F2- prognostic factors, a1- the total duration of the act of "obnyukhivanie" (C), a2- the total duration of the act of "sitting" (C), a3- the total duration of the act of "locomotion" (C), a4- the number of vertical racks, a5the number of acts "freezing", and when F1 is greater F2, make a conclusion about the absence of cognitive impairment in experimental animals, and if F2 is greater F1 ascertain the presence of cognitive impairment in experimental animals.
2. The method according to p. 1, characterized in that in the presence of cognitive impairment in experimental animals to assess the degree of their intensity according to the formula:
where PI is the prognostic index,
e - base of natural logarithm (2,72...),
F1and F2- prognostic factors
when PI is more than 0.5 and less than 0.64 severity of cognitive impairment is defined as low, if PI is more of 0.65 and less 0,84 medium, when PI is greater 0,85 - like high.
SUBSTANCE: device comprises a 3-litre flat-bottom cylindrical glass vessel (1), a neck of which is slightly elongated and narrowed, and has a rounded hole of 90 mm in a diameter. There is a screw thread for the tight fixation of the lid (2) on an outer surface of the elongated portion. What is also provided is a semi-sphere cup-shaped metal container (3) having a volume of 0.03±0.01 l, a thickness of 0.5 mm, on the bottom perimeter of which there are five holes of the diameter of 2 mm at a distance of 5 mm from each other; a cylindrical metal base (4) having a thickness of 0.5 mm, a height of 115 mm, a base diameter of 75 mm, and an apex diameter of 75 mm; on a side surface of the metal base, there are two parallel rectangular holes 75×45 mm. The container (3), wherein the combustion process takes place, is fixed on the base apex. What is also provided is a rectangular wooden base (5) 200 mm long, 15 mm wide and 3 mm thick, the front surface of which is provided with a rigidly fixed semi-circle metal collar (6) of the diameter of 150 mm with the glass container (1) tightly fixed.
EFFECT: invention provides the development of a biomodel poisoning with gas products of wood combustion.
3 dwg, 1 ex
SUBSTANCE: invention relates to medicine and biology and can be used for studying physiological functions of absorption of various substances and motility in an isolated section of the small intestine by Thiry-Vella in experiment. The abdominal cavity is opened. A purse-string suture in the form of a circle is made on the anterior surface of the stomach. In the centre of the purse-string suture the stomach wall is dissected. A guide catheter with a ball at the end is introduced into the small intestine lumen through the stomach cavity to the place of an anastomosis formation. The small intestine with the mesentery is intersected. After isolation of an isolated section of the small intestine a bolus-tube is fixed on the guide catheter with the ball at the end. An enteroenteroanastomosis between proximal and distal ends of the small intestine on the bolus-tube is formed. Integrity of the intestine and its impermeability are recovered by the application of single-row interrupted serous-muscular-subcutaneous sutures. The guide catheter with the ball at the end and the bolus-tube are removed through an opening in the stomach limited by the purse-string suture. Impermeability and consistency of the enteroenteroanastomosis are checked. Fistula tubes are fixed on the proximal and distal ends of the isolated section of the small intestine.
EFFECT: method makes it possible to preserve the normal lumen of the small intestine in the formation of the enteroenteroanastomosis "end-to-end" in small laboratory animals and check consistency and impermeability of the formed anastomosis intraoperationally, as well as to preserve the created model for a long time for studying small intestinal functions due to the application of the purse-string suture on the stomach, application of the guide-catheter with the ball at the end and the bolus-tube, and reliable intestinal sutures.
1 ex, 5 dwg
SUBSTANCE: method involves administering insulin-producing cells in a dose of 500 thousand cells in a solution of isotropic gel 0.5 ml, 15% ascorbic acid 10 mcl and 0.1% hydrogen peroxide 10 mcl. The preparation is administered subcutaneously into theanterior abdominal wall in a projection of the pancreas.
EFFECT: method reduces the number of complications related to developing tissue incompatibility.
SUBSTANCE: anthracosilicosis is simulated by a 4-hour inhalation exposure of coal-rock dust on animals 5 times a week. Gas-oil coal of the concentration of 50 mg/m3 at a dust particle size of 5 mcm is used. The disease respective to a 3-year miners' experience is simulated by 3-week coal poisoning of the animals. The disease respective to a 5-10-year experience is simulated by 6-week poisoning. The disease respective to more than 10 years of experience is simulated by 12-week poisoning.
EFFECT: creating the most reliable environment simulating the miners' working conditions, and enabling providing the experimental early changes specific for anthrasilicosis for the further determination of the time and methods for prevention thereof.
1 tbl, 1 ex
SUBSTANCE: invention can be used for studying pathogenesis mechanisms of combined radiation injuries (CRIs) including the mutual burdening phenomenon, as well as testing new methods and aids for preventing and treating CRIs. That is ensured by the CRI simulation by the sequential radiation exposure on rats. Total γ-radiation is first provided. That is followed by local beta-radiation covering a hair-free area of the animal's skin area isolated by a screen. A degree III-a burns are simulated by the use of beta-radiation in doses 30 and 60 Gy generated by a contact ionising radiation source having an activity of 24.3 mKi (900 MBq), an average radiant energy of Eav=1.4 MeV at a dose rate at the skin surface of 2.1 Gy/min triple attenuated through the skin thickness.
EFFECT: method enables the experimental reproduction of the radiation disease and the degree III-a superficial radiation burns equal in severity, and studying the effect of superficial skin injuries on the clinical course and outcomes of CRIs depending on the total radiation doses selected.
1 ex, 1 dwg
SUBSTANCE: invention refers to medicine. Implementing the method involves presenting an audio signal in the form of a superposition of separate tone components of input multiple-modulated vibration generated by an overlap of several acoustic vibrations. The vibration is processed on a signal processing model in the outer, middle and inner ear. The signal processing model in the outer ear is presented by a broad-band amplifier with an average amplification frequency of 3 kHz. The signal processing model in the middle ear is presented by a parametric system, wherein one of its reactive elements changes with time synchronously with the parameter variations of the multiple-modulated vibration. The signal processing model in the inner ear is presented by a dispersive delay line, the principle of action of which is based on the elastic audio wave velocity and frequency relation.
EFFECT: invention enables providing the more accurate detection of the biophysical processes implementing the hearing mechanism of the periphery portion of the acoustic system by interpreting it into an electronic analogue.
SUBSTANCE: invention refers to experimental medicine and can be used for examining diabetes mellitus, as well as in developing new methods of treating changes caused by type I diabetes mellitus. That is ensured by simulating alloxan diabetes in white outbread rats. Developing subcompensated diabetes mellitus is ensured by administering an alloxan solution in the rats intermittently intraperitoneally on an empty stomach, successively in doses of 5 mg/100 g, 7 mg/100 g and 5 mg/100 g of the animal's weight every 7 days, and developing decompensated diabetes mellitus is provided by three administrations of the alloxan solution in a dose of 10 mg/100 g every second day.
EFFECT: method provides higher probability of successful repetition and predictability of stimulating this disease by modifying the model corresponding to its subcompensated and decompensated forms.
SUBSTANCE: invention refers to simulation in medicine, and can be used for simulating the female genital diseases experimentally. Endometriosis is simulated by taking rectangular portions 2×3mm of the endometrium for auto-grafting and anchoring with the use of microsurgical instruments and injury-free synthetic non-absorbable mono-filament suture 9/0 in four sutures in the corners of the endometrial portions in the form of two double knots in each suture. Chronic endometritis is simulated by fixing the endometrial portions with the general surgical instruments and the synthetic non-absorbable suture material 4/0 in four sutures in the corners of the endometrial portions in the form of three knots in each suture; before and after surgical intervention, oestrogen is administered into the animal according to the following regimen: 0.1% synestrol 1.0 ml every second day intramuscularly once a day; 7 days before the auto-grafting, on the 7th, 5th and 3rd days, on the pre-operative and operative day, on the post-operative days synestrol is administered in the same single dose on the 2nd, 4th and 6th days.
EFFECT: method enables reducing the time of simulating the model.
SUBSTANCE: invention refers to medicine, namely to experimental surgery, and can be used for monitoring functional and pathomorphological changes accompanying intra-abdominal pressure (IAP). Simulating the IAP is ensured by puncturing and catheterising the abdominal cavity with a catheter ended with an elastic cuff in anaesthetised animals. The catheter is fixed on the animal's back skin. A spinal assistant is put on the abdomen so that the animal can freely move. A medium is pumped through the catheter into the cuff to achieve the desired IAP.
EFFECT: being simple and reliable, the method enables monitoring the animals experimentally and simplifying the research of intra-abdominal hypertension syndrome.
5 cl, 1 dwg
SUBSTANCE: invention refers to experimental medicine and concerns modelling Alzheimer disease. That is ensured by using B6C3-Tg(APPswe,PSEN1dE9)85Dbo/J oncomice. A preparation containing a synthetic analogue of aspartic acid isomerised in an amino acid residue in position 7 of human beta-amyloid with the amino acid sequence DAEFRH[isoD]SGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA) and/or fragments thereof containing the isomerised aspartic acid residue in position 7 [isoD] into the animal's blood-vascular system. The preparations are administered in a dose of 100 mcg, in 200 mcl/head of the solution once a month.
EFFECT: method enables a spontaneous change of a rate of the pathological process progression in the test animals depending on the purposes of the specific study by varying the number of injections and/or an amount of synthetic peptide per one injectable dose.
1 dwg,1 tbl
SUBSTANCE: invention refers to medicine, namely to preventive medicine, and aims at detecting young individuals suffering from a high risk of cardiovascular diseases for the timely correction. The individuals are presented with a questionnaire to detect major risk factors of the cardiovascular diseases in accordance with the National Cardiovascular Disease Prevention Guidelines. The questionnaire results are appraised by points: if observing psychological stress 3.01-4 for males and 2.83-4 for females, 0 points are assigned; 2.01-3 for males and 1.83-2.82 for females, 1 point is assigned; 2 and less for males and 1.82 and less for females, 2 points are assigned; if the respondent doesn't smoke, 0 points are assigned; if he/she smokes less than 1 cigarette a day, 1 point is assigned; smoking 1 and more cigarettes a day implies assigning 2 points; if the patient intakes ethanol in an amount of 13.7 g a day and less, 0 points are assigned; an intake of ethanol in an amount of 13.8 g to 27.4 g a day and less enables assigning 1 point; using ethanol 27.5 g and more requires assigning 2 points; if blood pressure is less than 129/84 mm Hg, 0 point is assigned; if the blood pressure value falls within the range of 130-139/85-89 mm Hg, 1 point is assigned, and 2 points are assigned, if the blood pressure value is 140/90 mm Hg or more; if a body weight index is 24.9 kg/m2 and less, 0 points are assigned; if the range is 25-29.9 kg/m2, 1 point is assigned; the value of 30 kg/m2 provides assigning 2 points; if the physical activity is accompanied by energy burning of 3 MET/min and more over six late months and more, 0 points are assigned; the physical activity accompanied by energy burning of 3 MET/min under six late months, 1 point is assigned; if the physical activity is accompanied by energy burning of less than 3 MET/min, 2 points are assigned; if the individual consumes more than 500 g of vegetables and fruit a day, 0 point is assigned; consuming less than 500 g provides assigning 1 point, and if a daily ratio contains no fruit and vegetables, 2 points are assigned; if a rest heart rate is 50 to 69 beats per minute, 0 points are assigned; the heart rate falling within the range of 70 to 79 beats per minute provides assigning 1 point, and the heart rate of 80 beats per minute and more ensures assigning 2 points; a negative past medical history of the cardiovascular diseases with manifested ischemic heart disease or cardiovascular diseases in the male relatives in the first degree aged 55 years old or less and in the female relatives in the first degree aged 65 years old or less shows assigning 0 point, while a positive past medical history of the cardiovascular diseases makes it possible to assign 1 point. The total score is derived, and if the calculated value is 8 points and more, the respondent is referred to a group of a high risk of cardiovascular diseases, and preventive measures are recommended.
EFFECT: method enables evaluating a risk of cardiovascular diseases in the young individuals by evaluating the risk factors.
1 tbl, 1 ex
SUBSTANCE: circle on the monitor screen is presented to the subject, on which a mark and a point object is placed. The point object moves at a predetermined speed along the circle. The testee stops the movement of the point object along the circle at the time of the proposed coincidence of its position with the mark by pressing the "Stop" button. Then the mismatch error of the point object and the mark is calculated. This is the time of trailing error with a positive sign or anticipation with a negative sign. After the predetermined time, the motion of the point object along the circle is resumed. The described procedure is performed the specified number of times. The order statistics of mismatch errors of the point object and marks is created. The range of the order is calculated by the formula: R=tmax-tmin. The ratio of the largest member of the order statistics tmax to the range R is calculated, multiplied by 100. The maximum absolute value of the prediction error |tpred.max| is determined. The lower quartile (25% quantile) of the maximum absolute value of the prediction error |tpred.max| is calculated. The percentage P of the absolute values of the prediction errors is determined, which are in the lower quartile of the maximum absolute value of the error |tpred.max|. Rating P is calculated as the reciprocal number of the arithmetic mean of the range R, the ratio of the largest member of the order statistics tmax to the range R, multiplied by 100, and the percentage Per of the absolute values of prediction errors which are in the lower quartile of the maximum absolute value of the prediction error |tpred.max|, multiplied by 100, according to the formula: P=100×1/(R+100×tmax/R+Per)/3=300/(R+100tmax/R+Per). The testee who has a higher rating is regarded as the most promising and able to show better results in upcoming competitions.
EFFECT: increasing reliability of determining the rating of combat athletes by evaluating the ratio of excitation and inhibition that characterises the speed capabilities and accuracy of motor actions.
10 dwg, 3 ex
SUBSTANCE: invention refers to sports medicine and aims at assessing a playing sportsman's physical actions accuracy. A sportsman being tested is presented a circle on a display with a mark and a point object moving at a specified speed round the circle. The sportsman being tested follows the point object travelling and stops it moving round the circle by pressing a Stop button once seeing an assumed matching of the moving point object and the mark. That is followed by calculating a matching error of the point object and the mark - a lag time with a positive sign or a lead time with a negative sign, and proceeding with moving the point object round the circle after some time. The sportsman being tested performs the described procedure for the specified times; a static series of the matching errors of the point object and mark is plotted; a range of the series is calculated, and a segment limited by the greatest and smallest members of the static series is marked on a number line. The physical actions accuracy of the sportsman being tested is assessed by a numerical line position of the segment limited by the greatest and smallest members of the static series of the matching errors of the point object and mark closest to a symmetry of a relatively zero point and the smallest value of the range of the matching errors of the point object and mark.
EFFECT: method enables providing the more reliable assessment characterising the playing sportsman's physical actions accuracy by instrumental measurements.
12 dwg, 4 ex
SUBSTANCE: invention refers to medicine and can be used for the correction of gynoid obesity. That is ensured by typing an eating disorder to be psychologically corrected. A diet therapy is prescribed with a caloric value determined by reducing a patient's habitual daily average caloric requirement per a percent equal to the patient's body weight index. That is combined with a low-carbohydrate diet with the carbohydrate content reduced to 40-60 g/day for 2 weeks. That is followed by prescribing a low-fat diet with the fat content reduced to 40-60 g/day for 10 weeks with avoiding fast assimilated carbohydrates having a glycaemic index of more than 50. The diet therapy cycle is repeated. That is combined with graduated aerobic physical exercises with no weight and resistance for the muscle groups of the lower body. There are also performed muscle-strengthening load exercises for the muscle group of the upper body promoting muscle fatigue. Between and after the graduated physical exercises, there are performed prolonged aerobic physical loads at least 5 days a week for 40-60 minutes. That is combined with the drug-free procedures aiming at improving the regional blood flow, increasing gluteofemoral and lower extremities muscle tonus. The low-fat diet involves administering health aids from the group of intestinal membrane-bound alpha-glucosidase and pancreatic alpha-amylase inhibitors. The course of the obesity correction involves administering catechin-containing preparations or biologically active additives. From the second month from the beginning of the obesity correction, the patient's face is exposed to full-spectrum natural or artificial light at intensity of not less than 2,000 lux for 1-2 hours a day simultaneously or alternatively with the physical loads.
EFFECT: method provides the effective weight reduction in the patients with gynoid obesity with reducing side effects.
5 cl, 8 tbl, 2 ex
SUBSTANCE: invention refers to medicine, forensic medicine, diagnostic measurements, including in investigative practice. An interactive psychophysiological testing (PPT) involves presenting a person being tested test questions, determining, analysing the psychogenesis parameters with using the person's physical parameter sensors, indicating the results and estimating. The test questions are typed as follows: first-version questions Q1, second-version questions Q2, neutral questions N. The questions Q1 and Q2 have an alternative meaning and equal power and are characterised by an equal presentation time, a consistency of comparing the questions according to the alternative versions, a minimised subjective personal effect of the PPT test expert by sound colour and level, an unconscious emotional support on the person's question perception, as well as an identity of putting the questions to be compared, their length and a fixation of the meaningful word and/or word combination in similar segments of both questions to be compared. The test is put in accordance with a concatenation as follows X:0→C,…,C→Q11.Q21→N→ →Q21.Q22→N→…→Q1n.Q2n→N, wherein X is a person's identification index; 0 is a non-estimated zero question; C is a question relieving an expectation stress; Q1i is the first-version question, wherein i=1, 2,…, n; Q2i is the second-version question, wherein i=1, 2,…, n; N is a neutral question; n is a number of specific circumstances of the event or action; ":", "→", "." are devisors. The questions are put taking into account the staging of the tested event, including the confirmed facts or data only and excluding the expert's conjectures or versions. The results are used to state one of the two alternative versions and to estimate the respective status of the person being tested. The psychogenesis is determined and analysed with using a polygraph, while putting the questions of the two alternative versions, indicating and processing the PPT data are conducted with a computer with relevant software.
EFFECT: method provides higher information value, accuracy, reliability, objectiveness of the PPT results as compared to the previously known tests up to 90-95% with avoiding the distortion and ambivalence of the results.
5 cl, 1 dwg
SUBSTANCE: invention refers to medicine, particularly to cardiology, and can be used for detecting individuals with a risk of the hypertensive disease. Regulatory relative risk of the disease, as well as standard multivariable personality test scores are determined for 10 years in apparently healthy males by the core examination, and a relation curve is plotted with using the scores: neurotic overcontrol - over 45 points, pessimistic attitude - over 49 points, rigidity - over 58 points, anxiety - over 67 points, and individuality - over 50 points. The scale scores are determined in the patient, and the curve is used to evaluate an individual relative risk of the hypertensive disease for the following 10 years in the patient.
EFFECT: method enables fast evaluation of the risk of this disease directly at the doctor's in a specific patient and detecting the individuals with the high risk of the hypertensive disease for the purpose of taking timely preventive measures.
1 tbl, 2 ex
SUBSTANCE: invention refers to medicine, namely to carrying out psychophysiological studies, e.g. analysing the individual's psychophysiological responses, and can be used in medical purposes, advertising industry, functional diagnostics, education science, psychology, judicial practice and criminal science. Current normal baseline physiological parameters of a person being tested are recorded by means of a polygraph. That is followed by forming a number of independent conceptual blocks of visual information to be presented to the person being tested on a demonstration monitor of an eye motion recording device to assess the subjective significance of the visual information. An eye position, motion, a path of motion and fixation lengths are recorded; zones of preferential passive concentration fixation are recorded. The derived data are analysed to select a primary gaze zone and to determine a gaze length. Visual information elements drawing much attention are determined; when presenting the visual information by means of the eye motion recording device, synchronous measurements of the physiological parameters of the person being tested are taken by using blood pressure contact sensors, photoplethysmographic measurement, galvanic skin response, upper and lower respiration pressure transducers, tremor sensors connected in turn to a sensor unit of a polygraph recording and processing the signals supplied from the sensors. The physiological parameter variations of the person being tested are assessed by comparing an excursion of their current values from the pre-recorded baseline values; the physiological parameter variations of the person being tested specific for perceiving the subjectively significant information and being simultaneous with the primary gaze moments are used to detect the visual information elements having the most emotional impact on the person being tested. Thereafter, the qualitative emotional assessment is ensured by distributing the detected visual information elements according to the pre-set parameters into a sequence with opposite coordinates in the form of verbal antonyms with using a semantic differential method.
EFFECT: method enables providing more accurate psychophysiological studies related to assessing the information perception, assessing the exposure of the visual information and its significance for the individual by recording the physiological parameters and using the semantic differential method.
SUBSTANCE: score rating of a medical history, neurological and psychiatric findings, and psychophysiological testing is carried out with assigning 0 points with no burdened medical history, focal symptoms and neurological clinical pattern of diseases, psychic diseases, unfavourable social environment observed. If observing one or two signs of mentioned above, 1 point is assigned. If more than three signs of mentioned above are observed, 2 points are assigned. A Luscher colour test is used to assess an autogenous norm, a vegetative and personal balance, a working capacity, a heteronomous or autonomous nature and a concentricity and excentricity. Emotionality, anger, joy and emotion control are measured by an emotional excitement scale. A degree of stress load is assessed by an Anderson life event scale. A simple hand-eye response is used to assess a functional level of the system, a response stability and a level of functionality. That is followed by detecting deviant trends by assigning 0, 1 or 2 points for each parameter depending on its value with all the points further summed up and no deviant trends in the behaviour diagnosed in case of 0 to 13 points collected; with a borderline case at 14-17 points; deviant trends in the behaviour at 18-26 points.
EFFECT: method enables objective assessment of the individual's behaviour by studying the neurological and psychiatric states and the psychological and psychophysiological findings.
6 tbl, 3 ex
SUBSTANCE: invention refers to medicine, namely to gastroenterology, and can be used for providing the higher clinical effectiveness in the patients suffering dyspepsia syndrome combined with overweight. The method involves measuring an anxiety level (HARS) and a depression level (HDRS) according to Hamilton rating scales, assessing nutritional status by bioimpedancemetry, a degree of manifestation of sleep disorders, measuring glucose, testing glucose tolerance, immunoreactive insulin, cholesterol, high-density lipoprotein (HDLP), triglyceride in venous blood. If the patient is observed to suffer dyslipidemia and impaired glucose tolerance or variations of immunoreactive insulin, the preparation Dibikor is administered in a dose of 500 mg a day for three months. The patient suffering dyslipidemia and both of the disorders stated above requires administering Dibikor 1000 mg a day for three months. If the patient suffers an overweight with a body weight index falling within the range of 27 to 29.9 standard units and an active cell mass (ACM) percentage of 45 to 55 per cent as shown by bioimpedancemetry, the preparation Dietressa is administered in a dose of 1 tablet 3-4 times a day for three to six months. If the patient suffers an overweight with a body weight index falling within the range of 27 to 29.9 standard units and an active cell mass (ACM) percentage of 45 to 55 per cent as shown by bioimpedancemetry, the preparation Dietressa is administered in a dose of 2 tablet 3-4 times a day for three to six months. If the patient suffers mild and moderate sleep disorders and shows an emotive type of eating behaviour combined with mild affective disorders, the anti-depressant Valdoxane in a single dose of 25 mg a day for the night for two weeks. The course of the disease is followed by the anxiety and depression levels two weeks after the beginning of the therapy, with the positive progression of the diseases, the 25mg dose of Valdoxane remains unchanged to the end of the therapeutic course for three to six months. If no progression is exhibited, the dose is to be increased to 50 mg a day, and the therapy is continued from four to eight months. If the patient suffers severe sleep disorders and shows an emotive type of eating behaviour combined with moderate affective disorders, the anti-depressant Valdoxane in a single dose of 50 mg a day for the night for two to three months.
EFFECT: in each specific case, the method enables providing faster reduction of the clinical symptoms, including body fat weight reduction and active cell mass and water normalisation, normalising circadian rhythms of the vegetative balance, as well as prolonging the remission.
3 tbl, 2 ex
SUBSTANCE: method employs a device in the form of a stand with two telescopic posts with induction sensors. A metal sphere moves on a channel at an angle of 5° and when the sphere passes the first induction sensor, an electrical signal is generated which triggers an electric stopwatch, and when the sphere passes the second induction sensor, the stopwatch stops. The second induction sensor is turned off and the test subject stops the stopwatch at the moment of seeing the sphere cross the finishing line by pressing a button. The reading of the stopwatch is the visual-motor reaction time. From 50 to 100 tests are carried out, which include recording all attempts by a test subject, including premature pressing of the button, constructing a personal bar chart for each test subject and selecting a standard criterion for evaluating and comparing the visual-motor reaction.
EFFECT: method increases accuracy and objectivity of evaluating visual-motor reaction to movement of an object in space.
4 dwg, 1 ex
SUBSTANCE: method involves forming signals as tests requiring solution. The tests are shown with frequency changed proportionally to the frequency they are solved. The number of tests is set to be the same in the cases of recovered and tired state. General amount of time spent for finding solution for given number of tests and the number of tests having right solutions are determined in each state. Mental fatigue degree is evaluated from relative change of mean time needed for finding the right test solution using a relationship like (Tm.r- Tm.t)100%/Tm.t, where Tm.t = Tsum.t/Kr.t, Tm.r = Tsum.r/Kr.r, Tm.t is the time spent for finding the right answer in tired state, Tm.r is the time spent for finding the right answer in recovery state, Tsum.t is the total time spent to solve given number of tests in tired state, Tsum.r is the total time spent to solve given number of tests in recovered state, Kr.t is the number of right answers to the tests in tired state, Kr.r is the number of right answers to the tests in recovered state.
EFFECT: reliable estimation of fatigue degree.