Composition with antioxidant and antibacterial activity
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine and represents a composition possessing the antioxidant and antibacterial activity and containing lithium ascorbate, differing by the fact that it additionally contains lithium benzoate in the following proportions, wt %: lithium ascorbate - 50; lithium benzoate - 50.
EFFECT: invention provides extending the range of antioxidants with antibacterial activity possessing normothymic activity.
1 ex, 2 tbl
The invention relates to the field of medicine, biology and pharmacology, and in particular to substances with antioxidant and antibacterial activity, and can be used in medicine, veterinary medicine, pharmaceutical industry to create on its basis the complex products, including for the purpose of correction of addictive disorders (as lithium salt) and related metabolic disorders. The claimed composition can be used as an integral part in various medicinal and pharmaceutical forms: ointments, balms, solutions and tablets.
It is known that lithium salts are widely used in medical practice, because the lithium cations have normotimicheskoe (psychostabilizers) activity, and the anion determines the bioavailability, solubility, toxicity and a number of other connection options, including antioxidant and antibacterial activity. Therefore, the selection of an appropriate anion and obtaining salt from a given set of properties is an important task.
As a prototype used antioxidant composition based on lithium salts having immunostimulatory activity (patent RU №2372081 from 25.07.2008, carpenters E. C., carpenter C. M.) containing ascorbate lithium aspartate lithium in percentage ratio of 50:50.
Ascorbate lithium, which is part of this comp is the exposure, is a bioactive drug with antioxidant and immunomodulatory activity, but its antibacterial activity in combination with lithium aspartate low. This reduces the potential range of applications of lithium ascorbate in various diseases. Thus, the main disadvantage of this composition is the lack of antibacterial activity.
Technical problem on which the invention is directed, is a composition with high antibacterial and antioxidant activity, containing lithium in soluble form.
The technical result of the invention consists in the expansion of the list of low-antioxidant with antibacterial activity, with normotimicheskoe action.
The problem is solved by obtaining the composition of the lithium salts of ascorbic and benzoic acids in the ratio, wt.%:
|benzoate of lithium||50|
The hallmark of the technical solution is used in the composition of lithium benzoate. Benzoate of lithium together with lithium ascorbate provides high antioxidant activity and antibacterial properties allow aemula of the invention.
The antioxidant activity of the claimed compositions in combination with the various components and the composition of the prototype was investigated using the method of cathodic voltammetry (E. I. Korotkov, Y. A. Kurbanov. Voltammetric method for determination of total activity of antioxidants. RF patent IPC G01N 33/00. No. 2002-115-232 from 06.06.02). About the size of antioxidant activity was assessed by the relative change of a current of oxygen reduction.
Determination of the antibacterial properties of the claimed compositions in combination with the various components and the composition of the prototype was carried out by assessing its minimum overwhelming concentration in relation to cultures of E. Coli and Str. Pyogenes.
An accurately weighed sample of 500 mg of ascorbate lithium is mixed with 500 mg of lithium benzoate, diluted in 100 cm3with distilled water. The resulting composition is examined for antioxidant activity by the cathodic voltammetry.
In the electrochemical cell was placed 10 cm3solution background electrolyte. As the background electrolyte used phosphate buffer (pH 6,86).
For collected three-electrode cell consisting of a working mercury-film electrode, the chloride of silver electrode is used as reference electrode and auxiliary electrode (HSA). The electrodes were immersed in the process the background electrolyte and connected to a voltammetric analyzer TA-2 (OOO NPP "Comunali", Tomsk). In this work, we used constant-current mode cathodic voltammetry, scanning speed capacity was W=40 mV/s, the operating range of potentials from 0 to -1,0 Century was Stirred solution using vibration electrodes 20, 10 - stage calming solution. Next shot the first wave cathodic oxygen reduction, dissolved in a background solvent, in this field potentials. Repeated shooting of voltamperometry at least three times. Later in the electrochemical cell was made an aliquot of 0.5 cm3of the sample composition and re-filmed voltamperometry under the same conditions.
According to the obtained results were building a graph of the relative current of oxygen reduction on the time of interaction of antioxidants (AO) with active oxygen forms. On the slope of the tangent to the straight-line segment of this curve was performed calculation of kinetic (kkinu(µmol/l·min)) criterion antioxidant activity. The antioxidant activity of the sample was determined by the formula:
where Ii is the current EV O2 in the presence of AO in solution, MCA, Io is the current EV O2 in the absence of AO in solution, MCA;
|The antioxidant activity of the claimed composition|
|Medication||Concentration, mg/cm3||Antioxidant activity, K, mmol/l·min|
|The claimed composition||0,5||1,54|
Determination of antibacterial properties was to determine the minimum overwhelming concentration of the composition (IPC), during which there is no growth of microorganisms. In test tubes was preparing a series of dilutions of the claimed composition and the prototype in mesopatamia broth (BCH) at concentrations of 20 mg/cm3, 10 mg/cm35 mg/cm3, 2.5 mg/cm3. Control was a test tube containing nutrient medium b is C bacterial suspension and the investigated compositions. Incubated the samples for 24 h at 37°C.
The minimum bactericidal concentration of the investigated compositions took the minimal concentration at which suppressed the growth of microorganisms. Controlling the growth of microorganisms was performed visually by the change in turbidity of the medium. To control the turbidity of the medium was used tube of bacterial suspension ("negative" control - without the analyzed compositions).
|The results of the study antibacterial activity|
|Studied composition||E. coli||Str. pyogenes|
|The concentration of the composition, mg/cm3|
|The claimed Composition||growth||growth||No|
|The absence of p is a hundred||growth||growth||The lack of growth||The lack of growth|
The study results of the claimed composition are presented in tables # 1 and # 2. From the materials table. No. 1 shows that the claimed composition has a pronounced antioxidant activity, the activity of the prototype and has antibacterial activity in doses of 20.0-10.0 mg/cm3significantly surpassing the activity of the prototype table. No. 2 (dilution method).
The claimed mixture exhibits antibacterial properties. Consequently it is possible to speak about the use of this composition as an integral part or as an independent drug from the group of salts of lithium (mood stabilizers) to prevent cell damage by free radicals and possess antibacterial activity. The claimed composition can be used as an integral part in different dosage forms.
Composition of antioxida the things and antibacterial activity,
including ascorbate lithium, characterized in that it further contains lithium benzoate in the following ratio, wt.%:
|benzoate of lithium||50|
SUBSTANCE: method for increasing the antitoxic body function in healthy individuals occupied in the chemical load environment involving administering an antioxidant water containing carrot juice, cod liver oil, olive oil, honey; the water is taken daily before the working shift for 10 days.
EFFECT: using the invention enhances the antitoxic body function by administering the water having high antioxidant properties.
1 tbl, 2 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: what is described is using a pharmaceutical composition in the form of an orally disintegrated tablet containing 6-methyl-2-ethyl-3-hydroxypyridine succinate as an active substance and crospovidone as a disintegrating agent in ratio 2:1 respectively, as a motor stimulating and anorectic agent.
EFFECT: preparing motor stimulating and anorectic agent.
16 dwg, 34 tbl, 13 ex
SUBSTANCE: for the purpose of post-surgical treatment of cerebral tumours, memantine is prescribed in a dose of 10 mg daily for 5-7 days before radiation therapy, and in a dose of 20 mg during radiation therapy 4-6 hours before the radiation session. On completing the course of radiation therapy, memantine is prescribed in patients aged up to 60 years old in a dose of 10 mg for 2-3 months daily, in patients aged 60 and older in a dose of 20 mg for 5-6 months daily.
EFFECT: method enables providing the higher patients' quality of life with a view to cancellation of glucocorticoids, ensuring higher clinical effectiveness ensured by an increase of the medullary substance to radiation.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to the pharmaceutical industry, namely to an agent with an antidyslipidemic and analgesic effect. The method for preparing the phytocomplex with the antidyslipidemic and analgesic effect, involving: a) grinding peeled bergamot fruit to prepare an undegraded mixture, b) introducing pectinolytic enzymes into the mixture; c) reducing pulp content; d) inactivating the above enzymes added at the stage b), to prepare a degraded mixture; e) performing ultrafiltration of the degraded mixture through membranes isolating the substances having a molecular weight of over 30,000 Da, to prepare a transparent solution; f) introducing the transparent solution on a polyphenol absorption column; g) washing the polyphenol absorption column with water and increasing pH to prepare an aqueous polyphenol fraction; h) transmitting the aqueous polyphenol fraction to cationic resin to recover the phytocomplex in an aqueous phase; i) drying the phytocomplex in the aqueous phase. The phytocomplex in the aqueous phase with the antidyslipidemic and analgesic effect. The phytocomplex with the antidyslipidemic and analgesic effect. A pharmaceutical composition with the antidyslipidemic effect containing the phytocomplex, and pharmaceutically acceptable additives. A pharmaceutical composition with the analgesic effect containing the phytocomplex, and pharmaceutically acceptable additives.
EFFECT: phytocomplex described above possesses the evident antidyslipidemic and analgesic effect.
SUBSTANCE: method involves preliminary intraperitoneal single administration of 5% aqueous alloxan in a dose of 15 mg/kg of body weight into a rat's body on an empty stomach. That is followed by administering afobazol under conditions of oxidative stress after observing the rat's blood glucose gain at least twice. Afobazol is administered subcutaneously in a dose of 10 mg/kg of body weight once a day for 30 days with underlying administration of L-arginine in a dose of 10 mg/kg of body weight or with underlying NG-nitroarginine methyl ester (L-NAME)-inhibitor of NOS-3 enzyme in a dose of 25 mg/kg of animal's weight.
EFFECT: method enables correcting the oxidative stress and NO-producing endothelial dysfunction accompanying vascular complications of diabetes mellitus.
1 dwg, 6 tbl, 1 ex
SUBSTANCE: invention concerns an antioxidant representing the amino acid glycine immobilised on the detonation-synthesised nanodiamond particles of 2-10 nm in size.
EFFECT: higher efficacy.
4 cl, 5 dwg, 7 tbl, 3 ex
SUBSTANCE: improving the functional result of a low resection of rectum in the patient suffering from rectum cancer is ensured by prescribing the drug preparation Laviocard+ 1 capsule 2 times a day with food for the pre-operative radiation course, one day before the operation and for 30 postoperative days to the extent of the low resection of rectum.
EFFECT: invention enables reducing a rate and degree of defecation and continence dysfunctions following the low resection of rectum and the radiation therapy.
1 tbl, 1 ex
SUBSTANCE: invention relates to compound for formula (1) , antioxidant, containing formula (1) compound or its salt as active ingredient, and to application of formula (1) compound or its salt for oxidant manufacturing. Invention also relates to formula (2) compound, which is intermediate for obtaining formula (1) compound.
EFFECT: formula compound, demonstrating antioxidant properties.
4 cl, 1 tbl, 13 ex
SUBSTANCE: invention refers to pharmaceutical industry, particularly to a method for increasing the radioresistance in mice. The method for increasing the radioresistance in mice consisting in the fact that 20-30 min before a radiation exposure, parsley juice diluted with normal saline is introduced intramuscularly.
EFFECT: method increases the radioresistance in mice effectively.
FIELD: medicine, pharmaceutics.
SUBSTANCE: present invention refers to medicine. A pharmaceutical formulation for the treating diseases associated with endothelial dysfunction contains an active ingredient presented by a methyl pyridine derivative - 1.0-6.0 wt %; purine - 10.0-80.0 wt % and additive agents - the rest. The active substance is presented by compounds of a group: 3 -(N,N-dimethyl carbamoyloxy)-2-ethyl-6-methylpyridinium succinate, 3-methylpyridinium succinate, 2-ethyl-6-methyl-3-hydroxypyridinium hydrochloride, 6-trichloromethyl-2-chloropyridine (nitrapyrin), 2-ethyl-6-methyl-3-hydroxypyridine succinate. Purine is presented by inosine, adenosine, hypoxanthine. The pharmaceutical formulation may be presented in the form of injections, lyophilisate, solid capsules, tablets and suppositories.
EFFECT: formulation according to the invention provides creating the stable drug dosage form which considerably exceeds the existing analogues in pharmacodynamics activity on the endothelial dysfunction and toxicological properties.
4 cl, 4 tbl, 9 ex
SUBSTANCE: method involves recovering choleragen anatoxin by tangential ultrafiltration with the use of a membrane with the rated cut-off of a molecular weight of 300 kDa, concentrating by tangential ultrafiltration with the use of the membrane with the rated cut-off of a molecular weight of 30 kDa and purifying by diafiltration with a triple volume of sterile purified water by tangential ultrafiltration with the use of the membrane with the rated cut-off of a molecular weight of 30 kDa.
EFFECT: higher yield of choleragen anatoxin, reduced time and number of technological stages for preparing it.
1 dwg, 2 tbl, 1 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to the pharmaceutical industry, namely to a method of obtaining a preparation, possessing an antimicrobial activity. The method of obtaining the preparation, possessing he antimicrobial activity, where an aboveground part of freshly harvested bear leek (Allium ursinum L.) or victory onion (Allium victoriale L.) is crushed to a paste-like state, poured with ethyl alcohol, infused tree times, under certain conditions, after each extraction the extracts are poured off, the obtained extracts are combined.
EFFECT: medication, obtained by the claimed method, possesses an expressed antimicrobial activity.
2 cl, 1 tbl, 6 ex
SUBSTANCE: pharmaceutical composition possessing antiviral and antibacterial activity and containing L-N-2,6-diamino-hexanoyl-D-glucosamine, L-y-glutamyl-cysteinyl-glycyl-proline, lysine glycyrisinate, desoxyribonuclease, palmitoyl hydroxypropyl trimmonium amylopectin/glycerin cross polymer, hydroxypropyl-beta-cyclodextrin, D,L-pyrrolidone carboxylate N-cocoyl ethylarginate, escin, an emulsifying agent, a preserving agent, a pH control agent, demineralised water in certain amounts. The pharmaceutical composition possessing antiviral and antibacterial activity and containing L-N-2,6-diamino-hexanoyl-ascorbyl phosphate, N-pyrrolidine-carbamoyl-D-glucosamine, lysine ursolate, ribonuclease, a modified acrylic thickening agent, hydroxypropyl-beta-cyclodextrin, D,L-pyrrolidone carboxylate N-cocoyl ethylarginate, glycyrrhizic acid, an emulsifying agent, a preserving agent, a pH control agent, demineralised water in certain amounts. The pharmaceutical composition possessing antiviral and antibacterial activity and containing L-N-2,6-diamino-hexanoyl-hinokitiol, N-pyrrolidine-carbamoyl-D-glucosamine, lysine betulinate, collagenase, palmitoyl hydroxypropyl trimmonium amylopectin/glycerin cross polymer, hydroxypropyl-beta-cyclodextrin, chlorhexidine bigluconate, D-panthenol, an emulsifying agent, a preserving agent, a pH control agent, demineralised water in certain amounts. The pharmaceutical composition possessing antiviral and antibacterial activity and containing fusidoyl lysine, lysyl-valyl-proline pomolate, proline escinate, lysozyme, hydroxypropyl trimmunium maltodextrin, cross polymer, hydroxypropyl-beta-cyclodextrin, D,L-pyrrolidone carboxylate N-cocoyl ethylarginate, cedar extract, an emulsifying agent, a preserving agent, a pH control agent, demineralised water in certain amounts.
EFFECT: compositions possess expressed antiviral and antibacterial activity and are stable.
4 cl, 2 tbl, 11 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: present invention refers to medicine, particularly to aspects covering antimicrobial compositions, and described antimicrobial compositions, antimicrobial silicone gel based on the above antimicrobial composition, a wound dressing and methods for preparing them. Among other things, the antimicrobial compositions contain at least one alkenyl- and/or alkynyl-substituted polysiloxane, at least one polysiloxane containing silicone-linked hydrogen atoms, and at least one hydroxylation catalyst, at least one hydrophilic ingredient, at least one silver salt.
EFFECT: invention can be used for preparing a drug preparation to be used in treating burns, scars, bacterial infections, viral infections and/or mycotic infections.
19 cl, 5 dwg, 8 ex, 6 tbl
FIELD: veterinary science.
SUBSTANCE: method involves intramuscular injections of a drug preparation. The drug preparation is presented by a mixture of a medical solution of formaldehyde and normal saline at the weight portion ratio (2-6):(994-998). First, the preparation is injected into ill, latently ill and healthy animals starting from 3-month-old calves in two doses of 5-6 ml every 7-8 days during the period from September to November. Thereafter, the injections are continued for the ill animals only, but for no more than 5 weeks, and 5-7 days after the last injection the remaining ill animals are detected and isolated.
EFFECT: method enables recovering the farm effectively from necrobacteriosis.
1 tbl, 5 ex
SUBSTANCE: group of inventions refers to veterinary science and aims at treating subclinical mastitis in cows. A preparation and a method for using it are declared. The preparation contains silver nanoparticles stabilised by submicrone titanium dioxide particles, poly-N-vinylpyrrolidone-2, and water in the following proportions, wt %: silver nanoparticles having a size of 200-300 nm 2.0×10-3, titanium dioxide particles having a size of 2-4 mcm 60.0×10-3, poly-N-vinylpyrrolidone-2 10.0, water - the rest up to 100.0. The method involves intracisternal introduction of the declared drug preparation in a dose of 10.0 ml twice a day for 2-4 days.
EFFECT: using the method enables providing the higher clinical effectiveness and reduces the length of recovery.
2 cl, 4 dwg, 7 tbl, 5 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: declared group of inventions refers to veterinary science and is applicable for treating the animals suffering from bacteriosis and yeast mycosis. A declared preparation contains oxytetracycline hydrochloride, sulphadimine, ampicillin sodium, nistatine, a solvent and the active substance conduit dimethyl sulphoxide, the quick-relief anaesthetic lidocaine in the following proportions, wt %: ampicillin sodium 4.0-8.0, oxytetracycline hydrochloride 2.0-4.0, nistatine 1.0-2.0, sulphadimine 2.0-4.0, novocaine 0.25-0.5, lidocaine 0.25-0.5, dimethyl sulphoxide 10.0-20.0, 1,2-propylene glycol - the rest. A method of treating the animals consists in administering the declared preparation in a dose of 0.1-0.2 cm3 per 1 kg of body weight.
EFFECT: using the declared group of inventions is high-efficiency for treating the animals suffering from bacteriosis and yeast mycosis and enables improving livestock farms with an unfavourable incidence of bacteriosis and yeast mycosis.
5 cl, 9 ex
SUBSTANCE: invention relates to a method of obtaining a polymer conjugate of an indolocarbazole compound of formula (I), where R1, R2, R3, W1 and W2 represent hydrogen, X represents methoxy-polyethyleneglycol. The method includes the interaction of a polymer compound of formula (II) with an indolocarbazole compound of formula (III), where Y stands for a methoxygroup. The nvention also relates to a polymer conjugate of formula (I), a pharmaceutical composition, containing the conjugate of formula (I) as an active ingredient, and to the application of the polymer conjugate of formula (I).
EFFECT: obtaining the polymer conjugate of the formula with a high output, the polymer conjugate of the formula for treatment of skin pathologies and HMGB1-associated pathologies.
48 cl, 7 dwg, 7 tbl, 15 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine, namely to broad-spectrum antibacterial preparations. What is presented is an agent representing sodium dihydroxocystine diaminodiargenate possessing the antibacterial activity. The agent represents a complex C6H12Ag2N2Na2O6S2 of two silver ions with a disodium salt of natural amino acid L-cystine being coordinately linked. The method for preparing the agent involves adding an estimated amount of argentous oxide to a solution of cysteine disodium salt, mixing a reaction mixture at room temperature until argentous oxide dissolves completely, and precipitating a target product in alcohol.
EFFECT: new agent, sodium dihydroxocystine diaminodiargenate possesses the evident antibacterial action on the pathogenic strains of Staphylococcus, Proteus, Salmonella typhimurium, Pseudomonas aeruginosa, and also inhibits the yeast growth, has low toxicity and storage-stability.
1 tbl, 4 ex
SUBSTANCE: invention refers to medicine, namely to dentistry, and can be used for the preventing and treating infectious processes accompanying dental surgeries. That is ensured by intraoperative administration of a therapeutic antibacterial preparation of penicillin and an antibacterial preparation of the third-generation cephalosporin. The above preparation is administered daily for 3-5 postoperative days. The above preparation is administered intra- and post-operatively once a day intravenously drop by drop for 15-35 minutes in a dose of 30-55% of the recommended daily dose.
EFFECT: invention enables reducing a possibility of antibiotic dosage with complete withdrawal of repeated intra- and post-operative one-day administration of the antibiotics, with avoiding the developing early and late postoperative infectious-inflammatory complications.
3 cl, 5 ex
SUBSTANCE: invention relates to a composition in the form of solid particles, including water-free crystalline 2-O-α-D-glucosyl-L-ascorbic acid, which includes 2-O-α-D-glucosyl-L-ascorbic acid in an amount, which is higher than 98.0% in the weight ratio, but less than 99.7% in the weight ratio, on the basis of a dry residue, which has a degree of crystallinity of water-free crystalline 2-O-α-D-glucosyl-L-ascorbic acid constituting 90% or more, counted on the basis of a diffraction image of X-rays for powder in the form of the said composition, which contains particles with the particle size, constituting less than 150 mcm, in the quantity, constituting 70 or more wt % of the entire disperse composition, and particles with the particle size, constituting at least 53 mcm, but smaller than 150 mcm, in the quantity, constituting from 40 to 60 wt % relative to the entire disperse composition, and which has a reducing ability of the entire composition, constituting less than 1 wt %. The said composition is used as a component of powder materials for food products, cosmetic preparations, therapeutic and preventive cosmetics and medications. The claimed invention also relates to a method of obtaining the claimed composition and to its application.
EFFECT: increased efficiency of the composition application.
11 cl, 7 dwg, 8 tbl, 7 ex