Method of manufacturing antimicrobial preparation from aboveground part of ramson

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to the pharmaceutical industry, namely to a method of obtaining a preparation, possessing an antimicrobial activity. The method of obtaining the preparation, possessing he antimicrobial activity, where an aboveground part of freshly harvested bear leek (Allium ursinum L.) or victory onion (Allium victoriale L.) is crushed to a paste-like state, poured with ethyl alcohol, infused tree times, under certain conditions, after each extraction the extracts are poured off, the obtained extracts are combined.

EFFECT: medication, obtained by the claimed method, possesses an expressed antimicrobial activity.

2 cl, 1 tbl, 6 ex

 

The invention relates to medicine and relates to methods of obtaining drugs from plant materials - Luca bear (Allium ursinum L.) and onion victorious (Allium victoriale L. (garlic), onion family (Alliaceae Juss.), which can be used as antibacterial agents.

Known volatile antibacterial agents derived from plant materials (plant): an alcohol tincture of onions (Allica") [Register of medicines of Russia. / Ed. by Y. F. Krylov.- M: Enfermagem, 1993. - S. 164.], tablets with garlic powder "Alist", liquid alcoholic extract of garlic "Allycat" and other [Mashkovsky M. D. Drugs: reference edition, 15th ed., Rev., Corr. and supplementary / Mashkovsky M. D. - M.: New wave, 2006 - S. 962]. The closest prototype is a liquid alcoholic extract of garlic "Allisat used as fungicidal, antimicrobial, anthelmintic, bacteriostatic agent, etc.

The method of producing a liquid alcoholic extract of garlic "Allycat" consists of the following: garlic bulbs clear of flakes and grind in a meat grinder. 34,6 kg garlic pulp is placed in a hermetically closed percolator, pour 52 l of 90% ethanol and insist 72 hours at room temperature. The resulting extract is drained, percolator pour the second portion of alcohol in the share of the ve 24 l and insisting again. After 72 hours, the extract is drained and insisting I repeat for the third time with 24 l of extractant. After 72 hours and plum extract the pulp press in the press and pressed attach to all extracts collected in a common sump. Settled within 48 hours, the extract was transferred into a mixer with anchor stirrer, add 0,228 kg of essential oils (caraway, dill and mint 0.3%), stirring the mixture for one hour with anchor stirrer. After mixing allisat filtered. Determine the content of dry residue and alcohol in the extract [Ant I. A., the Technology of medicines: 2 Tons / Ant I. A. - M., 1980. - T. 1. - S. 212-213].

The list of extracts for internal use, derived from plant material of the species Allium, have antibacterial properties, is limited. The disadvantages of garlic extract "Allisat are: the duration of the extraction (216 hours), moderate antimicrobial activity. However, the list of drugs that have antibacterial properties, is limited due to the lack of an adequate base of the plants. Therefore, expanding Arsenal of preparations with antibacterial properties, is an important task.

The technical object of the present invention is to develop a method of obtaining funds from the aerial part fresh garlic (Luca bear Allium ursinum l. or Luke victorious - Allium victoriale, family onion Alliaceae) with a duration of extraction 12 hours with high antibacterial activity.

100 kg of the above-ground portion of freshly picked wild garlic (Luca bear - Allium ursinum L. or onion victorious - Allium victoriale L. the onion family (Alliaceae)) are ground in a meat grinder and placed in a hermetically closed percolator. Raw material is poured ethyl alcohol 40% in the ratio of raw materials-the extractant is 1:1.5 (150 l) and infuse at room temperature for 4 hours. Then, the resulting extract is drained. In percolator pour a second portion of ethyl alcohol 40% in the ratio of raw materials-extractant 1:0.75 in (75 l) and insisting again at room temperature for 4 hours. Then, the resulting extract is drained. Insisting I repeat for the third time with ethyl alcohol 40% in the ratio of raw materials-extractant 1:0.75 in (75 l) and insisting again at room temperature for 4 hours. The hood is drained, connect with the first and second extracts. The fiber is squeezed in the press and centrifuge attached to the extracts collected in a common sump, get 385 l hood. Extract assert within 48 hours and check the content of dry residue. Hydroalcoholic extracts of garlic are translucent liquid from light to dark green in color, with a characteristic garlic odor and burning taste.

The resulting extrac the s investigated for antimicrobial activity.

The experimental results presented in examples 1-6. Use as a solvent of ethyl alcohol 40%, 70%, 90% ensures maximum extraction of active substances. Temperature, duration of extraction and extractive agent identified experimentally and guarantee the optimality of the process.

Examples of specific implementation method

Example 1

100 kg of the above-ground portion of freshly picked wild garlic (Luca bear - Allium ursinum L.) are ground in a meat grinder and placed in a hermetically closed percolator, pour ethyl alcohol 40% in the ratio of raw materials-the extractant is 1:1.5 (150 l) and insist 4 hours at room temperature. Then, the resulting extract is drained. In percolator pour a second portion of ethyl alcohol 40% in the ratio of raw materials-extractant 1:0.75 in (75 l) and insisting again. After four hours the extract is drained. Insisting I repeat for the third time in the ratio of raw materials-extractant 1:0.75 in (75 l). After four hours the hood merge, combine with the first and second hoods in the sump. The fiber is squeezed in the press and centrifuge attached to the extracts collected in a common sump, get 385 l hood.

In parallel to determine the moisture content of raw materials. All measurements are carried out with accuracy is 0.0002, Completeness of extraction is controlled by the total content of oxidizable compounds and content of aniu dry residue (3,26%).

Example 2

100 kg of the above-ground portion of freshly picked wild garlic (Luca bear - Allium ursinum L.) are ground in a meat grinder and placed in a hermetically closed percolator, pour ethyl alcohol 70% in the ratio of raw materials-the extractant is 1:1.5 (150 l) and insist 4 hours at room temperature. Then, the resulting extract is drained. In percolator fill the second portion of ethyl alcohol 70% in the ratio of raw materials-extractant 1:0.75 in (75 l) and insisting again. After four hours the extract is drained. Insisting I repeat for the third time in the ratio of raw materials-extractant 1:0.75 in (75 l). After four hours the hood merge, combine with the first and second hoods in the sump. The fiber is squeezed in the press and centrifuge attached to the extracts collected in a common sump, get 385 l hood. In parallel to determine the moisture content of raw materials. All measurements are carried out with accuracy is 0.0002, Completeness of extraction is controlled by the total content of oxidizable compounds and solids (2,83%).

Example 3

100 kg of the above-ground portion of freshly picked wild garlic (Luca bear - Allium ursinum L.) are ground in a meat grinder and placed in a hermetically closed percolator, pour ethyl alcohol 90% in the ratio of raw materials-the extractant is 1:1.5 (150 l) and insist 4 hours at room temperature. Then, the resulting extract is drained. In percolator fill in the second paragraph is a resource center ethyl alcohol 90% in the ratio of raw materials-extractant 1:0.75 in (75 l) and insisting again. After four hours the extract is drained. Insisting I repeat for the third time in the ratio of raw materials-extractant 1:0.75 in (75 l). After four hours the hood merge, combine with the first and second hoods in the sump. The fiber is squeezed in the press and centrifuge attached to the extracts collected in a common sump, get 385 l hood. In parallel to determine the moisture content of raw materials. All measurements are carried out with accuracy is 0.0002, Completeness of extraction is controlled by the total content of oxidizable compounds and solids (2,15%).

Example 4

100 kg of the above-ground portion of freshly picked wild garlic (onions victorious Allium victoriale L.) are ground in a meat grinder and placed in a hermetically closed percolator, pour ethyl alcohol 40% in the ratio of raw materials-the extractant is 1:1.5 (150 l) and insist 4 hours at room temperature. Then, the resulting extract is drained. In percolator pour a second portion of ethyl alcohol 40% in the ratio of raw materials-extractant 1:0.75 in (75 l) and insisting again. After four hours the extract is drained. Insisting I repeat for the third time in the ratio of raw materials-extractant 1:0.75 in (75 l). After four hours the hood merge, combine with the first and second hoods in the sump. The fiber is squeezed in the press and centrifuge attached to the extracts collected in a common sump, get 385 l hood.

In parallel, the definition is given in the humidity of the raw material. All measurements are carried out with accuracy is 0.0002, Completeness of extraction is controlled by the total content of oxidizable compounds and solids (2,13%).

Example 5

100 kg of the above-ground portion of freshly picked wild garlic (onions victorious Allium victoriale L.) are ground in a meat grinder and placed in a hermetically closed percolator, pour ethyl alcohol 70% in the ratio of raw materials-the extractant is 1:1.5 (150 l) and insist 4 hours at room temperature. Then, the resulting extract is drained. In percolator fill the second portion of ethyl alcohol 70% in the ratio of raw materials-extractant 1:0.75 in (75 l) and insisting again. After four hours the extract is drained. Insisting I repeat for the third time in the ratio of raw materials-extractant 1:0.75 in (75 l). After four hours the hood merge, combine with the first and second hoods in the sump. The fiber is squeezed in the press and centrifuge attached to the extracts collected in a common sump, get 385 l hood. In parallel to determine the moisture content of raw materials. All measurements are carried out with accuracy is 0.0002, Completeness of extraction is controlled by the total content of oxidizable compounds and solids (2, 25%).

Example 6

100 kg of the above-ground portion of freshly picked wild garlic (onions victorious Allium victoriale L.) are ground in a meat grinder and placed in a hermetically closed percolator, pour ethyl alcohol is 90% in the ratio of raw materials-the extractant is 1:1.5 (150 l) and insist 4 hours at room temperature. Then, the resulting extract is drained. In percolator pour a second portion of ethyl alcohol 90% in the ratio of raw materials-extractant 1:0.75 in (75 l) and insisting again. After four hours the extract is drained. Insisting I repeat for the third time in the ratio of raw materials-extractant 1:0.75 in (75 l). After four hours the hood merge, combine with the first and second hoods in the sump. The fiber is squeezed in the press and centrifuge attached to the extracts collected in a common sump, get 385 l hood. In parallel to determine the moisture content of raw materials. All measurements are carried out with accuracy is 0.0002, Completeness of extraction is controlled by the total content of oxidizable compounds and solids (2,11%).

In the study of antimicrobial properties of the target product were studied:

1) extracts of garlic (Luca bear - Allium ursinum L.) alcoholic liquid obtained by using 40%, 70%, 90% ethyl alcohol,

2) extracts of garlic (onions victorious - Allium victoriale L.) alcoholic liquid obtained by using 40%, 70%, 90% ethyl alcohol,

3) garlic extract liquid alcoholic "Allycat".

In the study of antimicrobial activity of the target product used method is based on the ability of drugs to diffuse into the agar, the infected test-cultures of microorganisms and inhibit the growth of the last [Handbook of microbio ogicheskom and virological research methods / edited Birger M. O. - M.: Medicine, 1982. - S. 172-177.].

Determination of the activity of the investigated extracts was performed in 3 Petri dishes of the same diameter with a flat bottom. In Cup, set horizontally, poured into 15 ml of molten nutrient agar. A layer of agar in a Petri dish filled in 1-2 ml of a suspension of the test organism in physiological solution. Then over the suspension were completely removed, dried the surface of the agar for 30 minutes. Then drill (6 mm diameter) were prosurvival 6 holes (wells) at a distance of 2.5 cm from the centre and the same distance from each other, the wells were filled with samples of the extracts and the solvent in the appropriate concentration (control). The Cup was left at room temperature for 30 minutes, after which they were placed in a thermostat at 37°C, without turning, horizontally, to get round the area. Under cover of the Petri dishes were placed sterile filter to prevent condensate wells. Zone of inhibition was measured after 16 hours. Evaluation of the results was performed according to the diameter of the zones of growth inhibition around the well, including diameter of the well:

- no zone of growth inhibition - test culture is not sensitive to this concentration of the drug;

the diameter of zone of growth inhibition of 10 mm - moderate sensitivity of the culture to a given concentration of p is eparate;

the diameter of zone of growth inhibition of more than 10 mm - high sensitivity of the test culture to a given concentration of the drug.

The results of the experiment are presented in table 1.

The research results presented in table 1 show that extracts of garlic (Luca bear - Allium ursinum L.), obtained using 40%, 70%, 90% ethanol, and the extracts of garlic (onions victorious - Allium victoriale L.)), obtained using 40%, 70%, 90% ethyl alcohol, have: a pronounced antimicrobial action against gram-positive cocci of the genus Staphylococcus: Staphylococcus aureus (209); Staphylococcus aureus (Type); Staphylococcus epidermidis Wood - 46, Salmonella gallinarum; Escherichia coli 055; spore-forming microorganisms of the genus Bacillus: Bacillus anthracoides - 96; Bacillus anthracoides; Bacillus subtilis, and Pseudomonas aeruginosa and Proteus vulgaris (table.1).

Control, liquid garlic extract "Allycat", showed moderate antimicrobial activity against Staphylococcus aureus (209); Staphylococcus aureus (Type); Staphylococcus epidermidis Wood - 46, Salmonella gallinarum; Escherichia coli 055; Bacillus anthracoides - 96; Bacillus anthracoides; Bacillus subtilis, Pseudomonas aeruginosa and Proteus vulgaris (table.1).

Table 1
The results of antibacterial activities of extracts of garlic
The extractantThe time of the extras the responses at each stage of extraction, hThe diameter of zone of growth inhibition of the test cultures of microorganisms mm
1234567891011
The extract of garlic (onion bear)
40%43230333228252829322829
70%42218121413121514191214
90%41010101012131715171113
The extract of garlic (onion victorious)
40%43129323329262728312930
70%42318121312121413181213
90%411 11111113141816181214
Control (garlic extract "Allycat")
90%729999991010101010
The test culture: 1. Staphylococcus aureus (209); 2. Staphylococcus aureus (Type); 3 Staphylococcus epidermidis Wood-46; 4 Escherichia coli 675; 5. Escherichia coli 055; 6. Bacillus subtilis L2; 7. Bacillus anthracoides - 96; 8. Bacillus anthracoides - 2; 9. Salmonella gallinarum; 10. Pseudomonas aeruginosa; 11. Proteus vulgaris.

1. The method of obtaining funds that have antimicrobial activity, characterized by the fact that the aerial part fresh onions bearish (Allium ursinum L.) are ground to a pasty condition, pour ethyl alcohol 40%, or 70%, or 90%, insist three times, for 4 hours at room temperature, prisutnost raw-extractant: at the first extraction is 1:1.5, the second extraction 1:0,75, the third extraction 1:0,75, after each extraction, the extract is drained, resulting extract combine.

2. The method of obtaining funds that have antimicrobial activity, characterized by the fact that the aerial part fresh onions victorious (Allium victoriale L.) is crushed to pasty state, pour ethyl alcohol 40%, or 70%, or 90%, insist three times, for 4 hours at room temperature, when the ratio of raw materials-extractant: at the first extraction is 1:1.5, the second extraction 1:0,75, the third extraction 1:0,75, after each extraction, the extract is drained, resulting extract combine.



 

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8 cl, 2 tbl, 48 ex

FIELD: medicine.

SUBSTANCE: method for increasing the antitoxic body function in healthy individuals occupied in the chemical load environment involving administering an antioxidant water containing carrot juice, cod liver oil, olive oil, honey; the water is taken daily before the working shift for 10 days.

EFFECT: using the invention enhances the antitoxic body function by administering the water having high antioxidant properties.

1 tbl, 2 ex

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