Method for differentiated immunomodulatory therapy of chronic tonsillitis and/or hypertrophy of palatine tonsils in preschool children
SUBSTANCE: pathogenetic treatment of chronic tonsillitis and/or hypertrophy of palatine tonsils in preschool children suffering from lymphoproliferative syndrome is ensured by the palatine tonsils debridement. An interleukin-1β(IL-1β) level is measured in the palatine tonsils washing. If the measured value is less than 5.8 pg/ml, recombinant interleukin-1β (IL-1β) is to be administered orally by phonophoresis with the use of the Tonsillor MM apparatus. Two courses of 10 procedures every 14 days are performed. The clinical effectiveness is assessed if observing a positive dynamics of IL-1β measured in the palatine tonsils washing 17 and 41 days after the beginning of the immunomodulatory therapy.
EFFECT: higher clinical effectiveness ensured by the differentiated selection of children for carrying out the immunomodulatory therapy, reducing a rate of infectious involvements of the palatine tonsils in the declared group of patients by the pathogenetically reasoned application of recombinant IL-1β.
3 cl, 2 tbl, 1 ex
The invention relates to medicine, namely to otorhinolaryngology and immunology, and is intended for pathogenetic treatment of chronic tonsillitis and/or hypertrophy of the Palatine tonsils in children of preschool age.
More than 80% of all respiratory diseases in children is accompanied by a lesion of the mucous membrane of the pharynx and lymphoproliferative rings. Chronic inflammation of the tonsils accompanied by inhibition of nonspecific factors of natural resistance of the organism, a violation of both humoral and cellular immunity.
In outpatient practice with the aim of increasing resistantanti organism is widespread non-specific immunostimulating agents of vegetable origin on the basis of Echinacea, ginseng, Rhaponticum carthamoides, chamomile, garlic, propolis and pantocrine. However, the use of systemic immunomodulatory drugs in Pediatrics without taking into account the points of application of the drug and pathogenetic bases of disease can lead to an imbalance of the immune system and the worsening of the disease.
As alternative ways of immunomodulatory therapy in chronic tonsillitis acquired methods of local effects on lymphoid tissue ring using various physical and instrumental methods, including directly the verbal high-frequency pulses, as well as delivery of drugs into the tissue of the tonsils with phonophoresis.
So there is a method of treatment of chronic tonsillitis, based on the introduction into the tissue of the tonsils using ultrasound drug "Malavit" (U.S. Pat. 2152773 Grew. Federation: IPC A61H 23/00, A61N 1/30. The method of treatment of chronic tonsillitis. No. 99102873/14; Appl. 18.02.99; publ. 20.07.00). The known method allows you to combine physical factors - low magnitude effect on the tissue of the tonsils and pharmacological effects "Malavita" on the lymphoid tissue of the tonsils. The main disadvantage of this method is the lack of criteria for selection of patients for this procedure, which severely limits the effectiveness of the method.
There is a method of treatment of chronic tonsillitis using ultrasonic sanitation Palatine tonsils apparatus "Tonsillar", where the medicinal product is used in a solution of 0.1% hydrogen peroxide followed by phonophoresis with ointment interferon-1α (Mechaniken N. In. and other Use of ultrasonic installation "Tonsillar" for the treatment of chronic tonsillitis. - M., 1984.). The method is based on rehabilitation and local delivery of anti-inflammatory cytokines in the tissue of the tonsils. The disadvantage of this method is its low efficiency and short-term effect.
poslednie studies have shown, that children with lymphoproliferative syndrome and chronic tonsillitis in mononuclear cells of the tonsils reduced level and/or functional activity of interleukin-1 beta (IL-1β). This condition is due to the prevalence of the homozygous mutant and heterozygous genotypes for the polymorphism SNP interleukin-1β (+3953 C->T).
Therefore, as the closest analogue to the claimed method the chosen method of local treatment of chronic tonsillitis recombinant interleukin-1β (beta Lakin) (Brody O. B. Clinical and immunological evaluation of the effectiveness of local treatment of chronic tonsillitis recombinant interleukin-1β (beta Lakin): author. dis. Kida. the honey. Sciences. - Saint-Petersburg, 2004. - 113 S.: ill.). The patients, before prescribing this group of patients underwent clinical and laboratory examination, and determined the concentration of cytokines in flush with the tonsils. Next was assigned treatment apparatus "Tonsillar" beta-Lakin duration of 5-8 treatments. The disadvantages of this method include the fact that it can be used only for patients aged from 14 to 42 years, with the rate of immunomodulatory therapy is prescribed to all patients without regard to their immunological status. In addition, the use of interleukin-1β in the form of a solution sposobstvuyuschim leaching it from the tissues of the tonsils and reduce therapeutic effect.
The technical result of the invention consists in a differentiated selection of children for immunomodulatory therapy, increase of efficiency of treatment of chronic tonsillitis and/or hypertrophy of the Palatine tonsils in children of preschool age, the reduction in the incidence of infectious lesions of the tonsils due to pathogenetically justified the application of recombinant interleukin-1β in gel base.
It is known that the lack of Il-1β upon antigenic stimulation leads to the violation processing and presentation of antigen to T lymphocytes and, consequently, to changes in priming T-helper cells towards activation of allergic inflammation (Immunology and Allergology, Ed. by A. A. Vorobyov, A. S. Bykov, A. C. Karaulova // Moscow, Practical medicine. - 2006. - 287 S.). While local and systemic allergic reactions determine not only the severity of chronic tonsillitis (compensation or decompensation), but also contribute to the development of hypertrophy of the tonsils, stimulating lymphoproliferative cells of the pharyngeal ring. On this basis, the increase in local levels of IL-1β in the Palatine tonsil in its original deficits in preschool children with chronic tonsillitis and/or hypertrophy of the Palatine tonsils is pathogenetically reasonable therapeutic approach. As a therapeutic drug b is l selected recombinant interleukin-1β-based gel (Kregel"). Phonophoresis of cytokines on the frequency of ultrasonic vibrations of 26.5±1.5 kHz leads to the penetration of regulatory peptide in the tissue of the tonsils without gross violations of the structure of its molecules. These data served as a starting point for a new way to treat chronic tonsillitis hypertrophy of the Palatine tonsils in children of preschool age.
To confirm the effectiveness of the proposed method, a survey of 121 children aged 5 to 7 years with chronic tonsillitis and/or hypertrophy of the Palatine tonsils II/III degree. Additionally inspected 109 conditionally healthy children of matched age, sex discreteness of the sample corresponding obsevational.
The concentration of interleukin-1β receptor antagonist interleukin-1, interleukin-4, tumor necrosis factor alpha and interferon-α in flush with the tonsils were examined by the method of enzyme-linked immunosorbent assay (ELISA) commercial kits LLC "Cytokine" , St. Petersburg according to the supplied instructions in the main program group and the comparison group. Flush with the tonsils were obtained by the method of lavage of Palatine tonsils 3 ml of physiological solution before treatment, and after the first and second courses of topical immunomodulatory therapy. The concentration of cytokines obtained in ELISA, were analyzed in PG/ml/p>
On a sample of conditionally healthy children (n=109) were defined standards of proinflammatory and proallergic interleukins rinse Palatine tonsils (Table.1).
|Norms of proinflammatory and proallergic interleukins in flush with the tonsils in children of early and preschool age|
|Alpha TNF, PG/ml||0,00||7,06|
|The receptor antagonist IL-1, PG/ml||0,00||1246,12|
|IL-1 beta, PG/ml||5,80||57,16|
|Alpha INFO, PG/ml||0,00||15,23|
|Note: Min - the lowest value in the group; Max - maximum value in the group; IL - interleukin; TNF - facto the tumor necrosis; INF - interferon.|
Thus, the level of IL-1β rinse the tonsils less than 5.8 PG/ml is the lower limit of the normal range and, as a consequence, an indication for topical immunomodulatory therapy with recombinant interleukin. Combined treatment was carried out by the apparatus of Tonsillar MM, with before treatment is carried out reorganization of the crypts of the tonsils, and the course of therapy consisted of two stages for 10 sessions with a 14-day break.
To study the effectiveness of topical immunomodulatory therapy in swabs from the tonsils in children with hyperproliferative syndrome was defined by the concentration of IL-1β:
- before treatment,
- 7 days after first treatment (17 days from the first sample is taken),
- 7 days after the second course (41 days after the first studies of the local and interleukins).
Based on these data it is proved that prior to treatment the mean level of IL-1β in the sample of children with lymphoproliferative syndrome was low to 4.2 PG/ml (±0,13), while the remaining indicators concentrations of interleukins defined within normal limits (Table.2). The latter fact is explained by the fact that the levels of interleukins in flush with the tonsils enough polymorphic: low to very high rates, respectively, and the normal range is also wide.
|Comparison of interleukin throat before treatment "Collegel" and after one and two courses local immunotherapy using apparatus "Tonsillar-MM"|
|Cytokines||Before the treatment||After 1 year||After 2 courses||p1||p2|
|The tumor necrosis factor-alpha, PG/ml||0,18||0,02||0,31||0,02||0,37||0,03||p>0,05||p>0,05|
|Receptor antagonist interleukin 1, PG/ml||165,3||17,9||145,5||15,8||116,4||a 12.7||P<0,05p<0,001|
|Interleukin-1 beta, PG/ml||4,22||0,13||a 4.83||0,14||5,95||0,11||p>0,05||p<0,05|
|Interleukin-4, PG/ml||13,77||of 1.34||10,75||0,85||or 10.60||1,02||p<0,05||p<0,05|
|Interferon alpha, PG/ml||10,32||0,85||9,13||0,74||7,30||0,60||p>0,05||p<0,05|
|Note: the table presents average values obtained on a sample of patients (n=121) with chronic tonsillitis, where M is the average value of the concentration of the cytokine, ±m is the average error variance, p1and p2the significance of differences in the compared groups of children after the first and second course of treatment, respectively.|
After the first year of the local shall immunomoduliruushimi therapy significantly decreased the indicators receptor antagonist interleukin-1 and interleukin-4 (receptor antagonist interleukin-1: before treatment 165,29±17,99 PG/ml and after treatment 145,46±15,83 PG/ml; for interleukin-4: before treatment 13,77±1,34 PG/ml and after treatment of 10.75±0,85 PG/ml). The average level of interleukin-1β rinse the tonsils after the first course local immunomodulatory therapy has increased, but no significant differences was not received.
After the second 10-day course of phonophoresis of recombinant IL-1β in the tissue of the tonsils dynamics of secretion of interleukins preserved. So the average level of the receptor antagonist Il-1 decreased to 116,37±12,67 PG/ml against 165,29±17,99 PG/ml before treatment (p<0.001) and against 145.46±15,83 PG/ml after the first course local immunomodulatory therapy (p<0,01). Decreased the average level of local interleukin-4 to or 10.60±1,02 PG/ml against 13,77±1,34 PG/ml before treatment (p<0.05) and did not change relative to the average after the first year of immunomodulatory therapy (p>0,05).
It should be noted that after two courses local immunomodulatory therapy with recombinant interleukin-1β in children of preschool age with chronic tonsillitis and/or hypertrophy of the Palatine tonsils rinse the tonsils significantly decreased the average level of interferon-α (10,32±0,85 PG/ml before treatment, 7,30 to±0,60 PG/ml after 2 courses of immunomodulatory therapy, p<0,05). After two courses of topical immunotherapy significantly increased the average level of IL-1β and was to be achieved 5,95±0,11 is g/ml (p< 0.05), and higher than the lower limit of the normal values given for interleukin swabs from the Palatine tonsils.
Thereby, conducted by topical treatment with recombinant interleukin-1β changed the composition of local interleukin towards activation processing and antigen presentation (increase of interleukin-1β), in the direction of suppressing allergic reactions (reduction of interleukin-4 receptor antagonist interleukin-1) and towards the end of the inflammatory process (decrease of interferon-alpha).
Due to the fact that after 2 courses of therapy are all indicators of interleukins changed towards normalization, we can assume that this method of treatment of chronic tonsillitis hypertrophy of the tonsils has a 100% pathogenetic characteristics efficiency.
Below is the method of implementation of the proposed method.
In children of preschool age with chronic tonsillitis and/or hypertrophy of the Palatine tonsils examine the level of IL-1β in the flushing of the tonsils. When values of IL-1β below 5.8 PG/ml conduct topical together with immunomodulating therapy, for this purpose, we first perform sanitation and washing caseous plugs using apparatus "Tonsillar MM, after which the applicator apply a thin layer of gel with recombinant interleukin-1β (Kregel") and spend phonophoresis on the frequency of 26.5±1.5 K is C for 30 seconds. Treatment with two courses of 10 days with a 14-day break through 17 and 41 days of starting treatment control levels of Pro-inflammatory and proallergic interleukins. However, the absence of dynamics in the levels of interleukins Palatine tonsils indicates no effect of the treatment and re-treatment procedures is not appropriate.
Thereby applies not technically difficult procedure local phonophoresis "Collegel" on the palatal tonsil apparatus "Tonsillar MM, not time-consuming method of treatment, can be used as monotherapy chronic tonsillitis hypertrophy of the tonsils.
Example 1. Child F., 5 years old, came to the immunologist doctor-allergist with complaints of frequent acute respiratory morbidity more than 8 times per year. When the examination of the throat revealed hypertrophy of the Palatine tonsils of the III degree. The study of interleukin-off from the tonsils revealed demotion: interleukin-1 beta to 4.2 PG/ml Were increased interleukin-4 to 29 PG/ml, All other indicators of interleukins were normal.
After two ten-day courses of therapy apparatus "Tonsillar-MM" with "Colegiales" the level of interleukin-1 beta has risen to 11 PG/ml, and the levels of Il-4 decreased to 24 PG/ml
Thereby conducted local immunomodulatory therapy day is "Colegiales" using device "Tonsillar MM normalized level of local interleukins, a study of the tonsils 5 months after the treatment showed a decrease in its size. The frequency of respiratory diseases for six months did not exceed 2 times.
Testing method conducted on the basis of CJSC "Modern medical technologies" 121 child under school age (5-7 years), suffering from hypertrophy of the tonsils and/or chronic tonsillitis. All patients received a 30-second phonophoresis "Colleges" on the Palatine tonsil using apparatus "Tonsillar-MM" in the course of 10 sessions. Two of them are of course phonophoresis got all patients. All children underwent phonophoresis with no complications. The effectiveness of local immunomodulatory therapy, according to local cytokine status, was observed in 119 children that was 98.34 per cent. The recurrence rate of chronic tonsillitis decreased from 119 children in 2 times.
1. The method of differential immunomodulatory therapy of chronic tonsillitis and/or hypertrophy of the Palatine tonsils in children of preschool age, including a study of the level of cytokines in the ELUTIONS from the crypts of the tonsils, reorganization of the Palatine tonsils and the local introduction of recombinant interleukin-1β (IL-1β) with phonophoresis apparatus "Tonsillar MM, characterized in that the treatment of children with lymphoproliferative syndrome is carried out at lower levels of Il-1β rinse Palatine is indolin less than 5.8 PG/ml, in doing so, a two course of 10 treatments at 14-day break, and the effectiveness of therapy is evaluated on the basis of positive dynamics of the level of IL-1β determined rinse the tonsils through 17 and 41 days from the beginning of immunomodulatory therapy.
2. The method according to p. 1, characterized in that the quality of drug from the group of recombinant interleukin-1β selected "Collegel".
3. The method according to p. 1, characterized in that phonophoresis "Colegiales" is carried out for 30 seconds at a frequency of ultrasonic vibrations of 26.5±1.5 kHz.
SUBSTANCE: implementing the method involves determining the content of biomarkers - matrix metalloproteinase 2 (MMP 2) and tissue inhibitor of metalloproteinase 1 (TIMP 1) in the patient's oral fluid sampled before or not earlier than 30 minutes following meals. Using the derived values enables diagnosing papilloma or cancer of patient's oral floor mucosa. Using the invention provides achieving a considerably higher accuracy of the differential diagnosis of benign and malignant new growths of the patient's oral floor mucosa on the day of vising, a considerably higher sensitivity of the differential instant diagnosis, a higher probability of detecting the processes of benign and malignant new growths in the patient's body both at the early stages of the disease, and at the later ones, as well as a considerably simpler preparation of the patient within certain time frames for sampling and storing the diagnostic material.
EFFECT: lower loss of sensitivity of the detection channel in the presence of multiple non-synchronous pulse noise and mutual interference.
3 cl, 5 ex
SUBSTANCE: invention represents a method for the Mycoplasma hominis DNA detection from a blood sample by dividing it into a supernatant and precipitate by centrifugation followed by enriching the analysed material, recovering DNA by affine sorption with a sorbent and a lysis buffer added, incubating, washing the sorbent precipitate three times by centrifugation and removing the supernatant, drying the sorbent precipitate, adding a DNA elution solution and detecting by the PCR method. The method is characterised by the fact that the material to be analysed is a whole blood precipitate and to be enriched while recovering DNA by doubling up a number of test tubes per one sample with the whole blood precipitate 100 mcl and the sorbent 20 mcl in each; before the washing procedure, each test tube is added with the washing solution 375 mcl, while the content of the test tubes is combined in one test tube with adding the DNA elution solution 40 mcl to the sorbent precipitate.
EFFECT: higher diagnostic accuracy.
SUBSTANCE: invention refers to medicine, and represents a method for the prediction of a risk of congenital infections by measuring specific Ig M and Ig G antibodies in a biological material, differing by the fact that the biological material is presented by the first-screening cervical smear at the 12th week of gestation; the smears are tested for the IgG antibodies to the rubella virus, cytomegalovirus, B19V parvovirus, toxoplasm viruses, type 1 and 2 herpes simplex viruses and an avidity of the specific Ig G to this agents; additionally, the same smear is tested for secretory non-specific Ig A by IFA to cytomegalovirus, Chlamydia, Mycoplasm antigens, and a genetic material of this microorganisms by PCR, and depending on the findings, groups of a high, moderate and low risk of congenital infections are predicted.
EFFECT: invention provides the more accurate prediction of the risk of the most actual congenital infections by the integral assessment of a collection of clinical anamnestic data, and the qualitative parameters of the laboratory findings at the first pregnancy screening.
SUBSTANCE: invention refers to medicine. The method involves a sequence of the qualitative measurement of an HLDF cell differentiation factor in the patient suffering from a cardiovascular disease.
EFFECT: sequence provides preparing the valid specific and reproducible immunoenzyme test system enabling detecting a vascular system biomarker in blood serum both of healthy individuals, and of the patients suffering from vascular diseases.
2 ex, 2 dwg
SUBSTANCE: invention refers to medicine, namely to gynaecology, and can be used for the prediction of recurrent carcinoma of the vulva. To this effect, treatment is preceded by examining the patient's blood for large granular lymphocytes (LGLs) and circulating immune complexes (CICs). If the LGL level is more than 11.8%, and the CIC level is less than 33.3 standard units, a recurrence-free period for 5 years and more is predicted. If the LGL level is less than 9.8% and the CIC level is more than 42.7 standard units, a recurrence progression for the following 2 years is predicted.
EFFECT: using the given method enables conducting the most effective therapeutic measures in the patients with unfavourable prognosis, as well as indicates a necessity of the regular careful case follow-up accompanied by the additive clinical-laboratory examinations.
SUBSTANCE: invention refers to medicine, namely to oncology, and can be used in treating soft tissue sarcomas for the prediction of recurrences. Substance of the invention consists in testing the cells expressing EGFR, CD 34, bcl 6, bcl 2, and if the cells with EGFR, CD 34, bcl 6, bcl 2 hyperexpression are determined , the short-term recurrence is predicted, while the cells with weak EGFR, CD 34, bcl 6, bcl 26 expression enables predicting the favourable outcome.
EFFECT: method provides the higher accuracy and information value of the prediction of recurrent soft tissue sarcomas.
2 ex, 1 tbl
SUBSTANCE: invention refers to medicine, namely to cardiology, and can be used to predict a risk of the cardiovascular mortality in patients with post-infarction chronic heart failure combined with type 2 diabetes mellitus. That is ensured by measuring circulating progenitors of the antigenic phenotype CD34+, CD133 dim, CD45-. If the measured cell count is less than 300 per one million leukocytes, a poor prognosis manifested by progressing heart failure and the increasing risk of the cardiovascular lethality is done.
EFFECT: invention provides predicting the survival rate values in the given group of patients by using the new blood biomarker.
2 cl, 2 dwg
SUBSTANCE: invention refers to medicine, namely to urology, and can be used for the intermittent therapy in patients suffering from benign prostatic hyperplasia (BPH). The patient is examined by clinical instrumental methods and prescribed with pharmaceuticals. The patient's total PSA and total testosterone in blood serum are measured before therapy involving Dutasteride 0.5 mg once a day and Tamsulosin 0.4 mg once a day and every 3 months after the therapy. A laboratory response rate marker
EFFECT: invention enables selecting the optimum length of treatment for each specific patient, reducing the length of therapy and drug load on the patient's body.
1 tbl, 2 ex
SUBSTANCE: presented invention refers to medicine, namely to the investigation of physical and chemical properties of biological fluids, and can be used for the prediction of chronicity of viral hepatitis B in children suffering from acute food allergy. The method for the prediction of the chronicity of viral hepatitis B in the children with food allergy involving clinical and laboratory examinations, including a biochemical blood assay for bilirubin, enzymes, and an enzyme immunoassay for hepatitis B markers at admission to hospital. The substance of the method: radioimmunoassay is used to determine blood serum HBs-antigenemia, gastrin, tripsin, total IgE, and cortisol, and if the derived HBs-antigenemia level is more than 15,000 r.im./ml, while the gastrin value is more than 50.0 ng/ml; the trypsin level is more than 450.0ng/ml; the total IgE value is more than 400.0 IU/ml, and the cortisol level is below 400.0 ng/ml, the chronicity of the disease is predicted.
EFFECT: firstly, the invention provides predicting the chronicity of hepatitis B at the early stages of the disease that provides advising effective therapeutic measures and increasing a rate of convalescence in the presented category of children; secondly, it enables diagnosing the functional activity of the upper digestive system and holding the well-timed measures to prevent its pathology from progressing.
13 tbl, 5 dwg, 1 ex
SUBSTANCE: invention may be used in therapy for differential diagnosis of the alcoholic liver disease (ALD). The technique involves serum analysis to measure serum secretory immunoglobulin A (ssIgA) and total immunoglobulin E (IgE) by enzyme-linked immunosorbent assay, and if the ssIgA concentration is more than 5.47 mg/l and the IgE concentration is more than 25 IU/ml, the alcoholic liver disease is diagnosed; if the ssIgA concentration is 5.47 mg/l or less, and the IgE concentration is 25 IU/ml or less, the non-alcoholic fatty liver disease (NAFLD) is diagnosed.
EFFECT: technique has high-sensitivity and specificity, requires no additional blood sampling procedures necessary for determining the most biochemical values measured in the patients with the suspected ALD, and it also enables differentiating the ALD and the NAFLD.
4 tbl, 4 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: present invention refers to biotechnology and represents a pharmaceutical composition applicable for treating a rheumatic disease and containing the humanised anti-CD4 antibody able to activate CD4+CD25+ regulatory T-cells, and methotrexat. The present invention also discloses a kit and a method of treating the rheumatic disease with using the above pharmaceutical combination and the above kit.
EFFECT: invention enables implementing more effective methods of treating the rheumatic diseases, including rheumatoid arthritis.
57 cl, 8 dwg, 2 tbl, 2 ex
SUBSTANCE: invention refers to medicine, namely to oncology, and concerns treating the patients suffering oncological diseases by administering the preparations inducing active cellular immune stimulation. The preparations are administered in the form of lyophilised lysate of gram-positive enteric bacteria enclosed in gelatine capsules. That is combined with correcting intestinal dysbacteriosis, administering antioxidants, vitamins and minerals, as well as nutrient-enriched protein food, amino acid L-arginine, and pectin- and fibre-enriched vegetable food.
EFFECT: method provides higher clinical effectiveness of the patients suffering the oncological diseases with no side effects.
SUBSTANCE: developed agent contains trophoblastic β-1-glycoprotein (TBG), immunoglobulin (Ig) and additionally contains tetrapeptide H-Tyr-X-Y-Glu-OH, wherein X - Gln and/or Glu, Y - Cys (acm) and/or Cys.
EFFECT: effective treatment of autoimmune diseases by single doses containing TBG 1 mg, Ig 19 mg and tetrapeptide 20 mg.
6 tbl, 13 ex
SUBSTANCE: group of inventions refers to veterinary science and aims at normalising metabolic processes, stimulating immune system and blocking mechanisms of infectious process with a risk of endogenous infection activation. A method for producing a combination immunometabolic preparation with anti-infectious activity involves dissolving succinic acid and levamisole in demineralised water with formalin added. According to the other aspect of the invention, the immunometabolic preparation additionally contains polyethylene glycol. The ingredients are used in the declared ratio.
EFFECT: using this group of inventions provides producing an injection form of the immunometabolic preparation with anti-infectious activity.
2 cl, 3 tbl
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to the chemical-pharmaceutical industry and represents a method of obtaining a complex preparation for the application in veterinary, possessing immunomodulating and antiseptic properties, which includes mixing succinic acid, levamisole and formalin in distilled water with the following component ratio, wt %: levamisole - 3.0%-3.5%; succinic acid - 2.0%-2.5%; formalin - 0.3%-0.5%, distilled water - the remaining part; sterilisation of the solution by autoclaving is performed in a mode of 1 atm for 20 minutes.
EFFECT: elaboration of the method of obtaining the complex preparation for the application in veterinary, possessing immunomodulating and antiseptic properties.
FIELD: medicine, pharmaceutics.
SUBSTANCE: claimed group of inventions relates to medicine, namely to therapy and immunology, and deals with treatment of autoimmune diseases. For this purpose 3-(5-(4-(cyclopentyloxy)-2-hydroxybenzoyl)-2-((3-hydroxy-1,2-benzisoxazol-6-yl)methoxy)phenyl)propionic acid or its salt is introduced in the form of a combination or a pharmaceutical composition with one or more of TNFα inhibitors.
EFFECT: invention provides efficient treatment of autoimmune diseases due to synergic action of the said components.
6 cl, 1 ex, 1 tbl
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to the pharmaceutical industry, namely to a personal preparation from earthworms for treatment of diabetes. The method of obtaining the personal preparation from earthworms for treatment of diabetes includes placement of sexually mature earthworms in a medium, consisting of organics, which additionally contains a solution of a patient's urine, after that, the sexually mature earthworms are removed and for 3 months each 7-10 days fresh organic food is added to the remaining mass, after that, the sexually mature earthworms are separated, placed in a reservoir and sprayed with an alcohol solution, mixed, poured with the alcohol solution and placed into dark place under the specified temperature, with the solution being mixed daily, with further separation of the extract from the sediment. The personal preparation for treatment of diabetes.
EFFECT: application of the described above preparation increases efficiency of diabetes treatment.
3 cl, 3 dwg, 1 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine, more specifically to a pharmaceutical composition possessing antithrombotic, thrombolytic, immunomodulatory, anti-inflammatory action, normalising lipid and carbohydrate metabolism, more specifically to the pharmaceutical composition of the substance Pijavitum (hereinafter referred to Pijavitum) made from lyophilised medicinal leech. The above pharmaceutical composition is presented in the form of an enteric coated tablet.
EFFECT: coating prevents the active ingredients of Pijavitum from destruction under action of the enzymes and acid medium of the stomach.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to a new antineoplastic drug representing 2-isobutyl-4,6-dimethyl-5-oxypyrimidine of the general formula I specified below. The drug may be used for an adjuvant antineoplastic immune therapy. The invention also refers to a method for preparing 2-isobutyl-4,6-dimethyl-5-oxypyrimidine. According to the method, isocaproic acid amide reacts in ice-cold acetic acid with chloracetyl acetone to form the respective oxazole which reacts with aqueous ammonia which leads to preparation of the above compound.
EFFECT: what is disclosed is a manifested tumour growth inhibition with no signs of the toxic effect on somatic characteristics of tumour-carrier mice.
3 cl, 9 tbl, 6 ex
SUBSTANCE: what is presented is a method for simulating a delayed hyperresponsiveness to mycobacteria bovis. Avirulent mycobacteria bovis of the strain BCG are administered intracutaneously to albino guinea pigs produced by consanguineous mating of brother x sister (F2); that is followed by forming a group (groups) of animals showing various extents of an inflammatory response in reaction to the intracutaneous administration of mycobacteria bovis of the strain BCG 30-35 days later.
EFFECT: method is effective in studying the mechanisms of the delayed hyperresponsiveness and assessing the efficacy of antituberculosis agents.
2 tbl, 5 ex
SUBSTANCE: present invention refers to medicine. The preparation contains the active substance furacilin (nitrofural, 2-[(5-nitro-2-furanyl)methylen]hydrazine carboxamide), the excipient - sodium chloride, a gas-forming mixture, an acidity regulator for reducing pH from 6.5 to 7.5 and a lubricant if needed. The gas-forming mixture consists of an organic acid and sodium hydrocarbonate. According to the method for preparing effervescent tablets of furacilin, furacilin and sodium chloride are milled in a combination; all the ingredients are placed into a mixer, and the prepared mixture is tabletted by direct compression. The antimicrobial therapeutic preparation of furacilin in the form of effervescent tablets is used for septic wounds, bed sores, small skin damages, including scratch marks, abrasions, cuts; infectious-inflammatory oral and throat diseases, including acute tonsillitis, stomatitis, gingivitis and tonsillitis. The effervescent tablets of furacilin are storage-stable for two years and water-soluble at room temperature for 5 minutes.
EFFECT: preparing the antimicrobial therapeutic preparation in the form of the effervescent tablets used for preparing the solution for local and external application.
4 cl, 6 tbl, 3 ex