Method of treating moderate house atopic bronchial asthma in patients with immune deficiency suffering from frequent acute respiratory infections
SUBSTANCE: treating moderate house atopic bronchial asthma in the patients with immune deficiency suffering from frequent acute respiratory infections is ensured by administering Grippferon intranasally 5 days a week with a leech therapy accompanied by a subcutaneous allergen-specific immune therapy with causally relevant allergens. The leech therapy is performed by applying one medical leech on the skin in a projection of the thymus twice a day for the period of time with no ARI episodes recorded over the last 3 months. The allergens are administered endolymphatically in the proximal direction from a cuff applied on a forehand, and pressure is maintained at 40 mm Hg for 60 minutes with an underlying basic therapy with the Symbicort preparation.
EFFECT: using the method enables providing the higher clinical effectiveness in anti-recurrent therapy of the frequent ARI episodes in the patients suffering from the moderate form of bronchial asthma, reducing the number and length of the ARI episodes, preserving control over the asthmatic symptoms with reducing a dose and a frequency of the preparations of the bronchial asthma therapy.
The invention relates to medicine, namely to clinical immunology and Allergology, and can be used in the treatment of partially-controlled and controlled moderate to severe forms of atopic bronchial asthma (BA) household etiology, occurring with frequent exacerbations in the background of the syndrome secondary immunodeficiency States (VIGS) in patients with frequent acute respiratory infections (ARI).
Currently, there is no doubt the fact that the most sensitive and integral indicator of health in humans, is the state of his immune system (is), because it provides both antigenic and structural stability, and regulation of the processes of proliferation and differentiation of immune cells. The status of IP is important in the formation reaction of the body to any external and internal effects, including frequent acute respiratory infections of the respiratory system.
There is a method of treatment of frequent acute respiratory infections in patients with VIDS, consisting of intranasal administration of the drug "Grapperon, nose drops (CJSC FIRN, M., Russia) (Gaponyuk P. J., E. Doroshenko M. Journal "Polyclinic №5 2008", pages 22-26).
The disadvantage of this method is its low efficiency due to the rapid elimination of the drug from the mucous membranes of the nose, and t is the train no effect on the system thymusdependent cell type of the immune response.
The closest achieved technical result is a method of treatment of atopic BA household etiology by conducting subcutaneous allergen specific immunotherapy (ASIT) cause significant allergens (FSUE "NPO "Microgen" the Ministry") in the period of remission or pharmacological control (Kolher P. C., 2010, 516 S.).
The disadvantage of this method is its low efficiency because of the dominating influence on the systemic immune response and the absence of direct stimulation of lymphoid formations associated with the mucous membranes of the upper respiratory tract.
The authors proposed an efficient method for the treatment of moderate atopic bronchial asthma household etiology in patients with immunodeficiency condition, suffering from frequent ARI, does not require expensive equipment and drugs, but to effectively arrest the frequent exacerbation of BA caused respiratory infections that are resistant to treatment with other topical and systemic immunotropic drugs.
The technical result of the proposed method is to increase the effectiveness of preventive therapy of recurrent episodes of acute respiratory infections in patients suffering from moderate form of BA, due to the step therapy scheme of the proposed method.
Technical re the query result is achieved by that is administered intranasally "Grapperon" for 5 days a week with the simultaneous holding of hirudotherapy in the form of imposing one medical leeches on the skin in the projection of the thymus gland, which is carried out 2 times a week, during the time when for the last 3 months not recorded episodes of acute respiratory infections, and allergens enter the endolymphatic proximal cuff placed on the forearm, and keep the pressure 40 mm RT. Art. for 60 minutes on the background of basic therapy drug "Symbicort".
The method is as follows.
Patients with atopic BA household etiology, under the control of the basic therapy, and partial loss due to frequent re-ORI associated with secondary immunodeficiency syndrome in the form of a reduction in the number of KIC and/or their effector functions, designate the following diagram.
"Grapperon" (CJSC FIRN, M., Russia) 3 nose drops 1 time per day, in the morning, 5 days a week with a simultaneous imposition on the projection area of the thymus gland for 1 (one) medical leech to inadvertent separation of 2 times per week. A bleeding wound is closed with a sterile swab. Treatment VIDS in this way shown to reduce the frequency of re-ORI to 1 times in 4 months (i.e. 3 or less times per year). At the same time carry out ASIT cause-and-meaningful/mi allergen/mi, BB is necessary(mi) hypodermic syringe with a gradual increase in the volume of injection distal to the cuff with supported within 60 min pressure 40 mm RT. Art. (endolymphatic) program recommended by the manufacturer allergens FSUE "NPO "Microgen": from 0.1 to 0.8 ml of the 10-5 dilution 1 time per day during the set dose, 0.5 ml of undiluted serum 1 time in 7-14 days. The course is carried out for 3-5 years.
By the present method treated 54 patients with good therapeutic effect in 48 people, which helped reduce the amount of basic therapy for GINA to step down within 1 year of treatment.
The treatment by the present method.
Nicholas P., 17, surveyed 20 November 2007. The patient is re-examined on 30 November 2010.
Clinical diagnosis: Bronchial asthma, atopic, moderate, partly controlled, domestic (house dust 4C, miracles house dust) etiology. Frequent acute respiratory infections. Secondary immune deficiency, cell type.
Complained of frequent episodes of acute respiratory infections - up to 10 times over the past year, lasting up to 2 weeks, accompanied by fever, General weakness and lethargy; recurrent sore throat.
Frequent respiratory infections marked the last 3 years since migrated lacunar angina.
With 10 years suffers from bronchial asthma controlled with inhaled corticosteroids and bronholitikami. At last the e 3, the state asthma control is not sustainable: episodes expiratory dyspnoea with distant whistling rales occur during each respiratory infections and continue for 3-5 days. In the last 3 years of monitoring of asthma symptoms was carried out by the drug "Symbicort" (ASTRAZENECA AB, Sweden) 4,5/160, - 1 dose × 1-2 times a day, and at exacerbation, SMART technology, up to 12 doses per day.
The General condition of the patient closer to satisfactory health does not suffer. The skin is clean. Zev calm, purulent overlay no. Symptoms Zack, Transfiguration, Koritskiy, Carnico - negative. Thorax slightly increased in the sagittal dimension, percussion sound over all lung fields has boxed shade, breathing slightly elongated and exhale single sibilant rales in the lower lung. NPV 21 in 1 min HR 91 in minutes From other internal organs and systems of pathological changes were noted. Immune status is set moderately expressed disorders of phagocytic and secretory functions of polymorphonuclear leukocytes with a reduced number of CD3+, CD4+, and CD8-positive lymphocytes and normal levels of major classes of immunoglobulins A, M and G. the Immunoglobulin E - 570 ME. In addition, it was noted saved spontaneous and reduced to phytohemagglutinin (PHA) proliferation of lymphocytes, as well as increased spontaneous and decreased stimulated production of tumor necrotizing factor alpha (TNFα) in cell culture. Performed during health skin-scarify which include samples verify itchy papules more than 15 mm pseudoprime to the allergen from the house dust 4 series and allergen Dermatofagoidus pteronissimus.
Was therapy according to the scheme of the proposed method.
"Grapperon" (CJSC FIRN, M., Russia) was administered to 3 drops in each nostril 1 time per day 5 days a week in the morning for 3 (6, 9, 12) months prior to the disappearance of episodes of ARI in the last 3 months. Subsequent emergence of ORI reception "Grapperon" (CJSC FIRN, M., Russia) was renewed and cancelled on the same criterion. At the same time, 2 times a week on the skin of the sternum in the area of projection of the thymus put 1 medical leech to inadvertent separation. Expose haemolymph ranged from 20 to 50 milliliters per day. Leeching was held for 3 (6, 9, 12) months to achieve a target frequency of episodes of ARI and reopened in their new appearance. At the same time held the allergen-specific therapy drugs from the house dust allergens 4C and Dermatofagoidus pteronissimus (FSUE "NPO "Microgen", Russia), mixed 1:1, according to the program recommended by the manufacturers, starting with the 0.1 ml of the 10-5 to 0.5 ml undiluted allergen entered endolymphatic distal imposed cuff saved 40 mm RT. Art. pressure for 60 minutes
In the first year of treatment, the patient underwent 4 episode ORI, accompanied by paroxysms expiratory dyspnoea with distant sibilant rale, which were cut short by Symbicort the (ASTRAZENECA AB, Sweden) 4,5/160 technology SMART to 12 inhalations per day for 2 days. No episodes of acute respiratory infection in last 3 months is allowed to cancel anti-relapse treatment "Gripperdon" (CJSC FIRN, M., Russia) with leeching. Over the next 6 months, the asthma control was carried out by Symbicort" (ASTRAZENECA AB, Sweden) and ASIT at a dose of 0.5 ml, administered endolymphatic 1 time in 2 weeks. Then the patient again underwent an episode of ARI, without exacerbation (out of control) asthma, and therefore treatment Gripperdon" (CJSC FIRN, M., Russia) and leeching was renewed for 3 months, during which episodes of ARI was not. Over the past year the patient has suffered 2 episodes of ARI, with retained control of asthma, and after the first episode was resumed treatment Gripperdon" (CJSC FIRN, M., Russia) and leech, within 3 months, during which episodes of ARI had not been registered and after which immunogenically therapy was cancelled. All this time I held ASIT household allergens with the frequency of the endolymphatic introduction 1 every 2 weeks.
In immune status with the re-examination installed: disorders of phagocytic and secretory functions of polymorphonuclear leukocytes no, the relative and absolute number of CD3+, CD4+, and CD8-positive lymphocytes are not changed. Normal levels of immunoglobulins main class is s - A, M and G. the patient was noted saved spontaneous and increased PHA proliferation of lymphocytes, as well as increased spontaneous and stimulated production of TNFα in cell culture. Performed during health skin scratch tests have registered the pimple less than 3 mm allergens from house dust 4 series and allergen Dermatofagoidus pteronissimus.
Thus, ongoing therapy led to a decrease in the number of acute respiratory infections, control of the symptoms of atopic bronchial asthma and restoration of immune status.
During 3 years of treatment with 7 episodes of acute respiratory infection (2 episodes in the last year - a marked reduction in the incidence of ARI), which lasts 3-4 days (decrease in the duration of exacerbations), full control of asthma symptoms and restore the settings of the immune system.
Claimed by the authors of the method for the treatment of moderate atopic bronchial asthma household etiology in patients with immunodeficiency condition, suffering from frequent acute respiratory infections, showed high efficiency, allowing you to reduce the number and duration of episodes of ARI, maintaining control of asthma symptoms, reducing the dose and frequency of use of drugs of basic therapy of bronchial asthma.
The advantage of the proposed method over the known is that the claimed ability is b can effectively reduce the frequency of exacerbations of asthma in patients with secondary immunodeficiency syndrome, which greatly increases the effectiveness of therapy of bronchial asthma.
A method for the treatment of moderate atopic bronchial asthma household etiology in patients with immunodeficiency condition, suffering from frequent acute respiratory infections through subcutaneous allergen specific immunotherapy cause significant allergens, characterized in that it further administered intranasally "Grapperon" for 5 days a week with the simultaneous holding of hirudotherapy in the form of imposing one medical leeches on the skin in the projection of the thymus gland, which is carried out 2 times a week, during the time when for the last 3 months not recorded episodes of acute respiratory infections, and allergens enter the endolymphatic proximal cuff placed on the forearm, and keep the pressure 40 mm RT. Art. for 60 minutes on the background of basic therapy drug "Symbicort".
FIELD: medicine, pharmaceutics.
SUBSTANCE: present invention refers to new phenylpyrimidone derivatives of formula I possessing the properties of a phosphodiesterase type 5 (PDE5) inhibitor. The compounds of formula I can be used for treating various vascular disorders, such as erectile dysfunction, pulmonary arterial hypertension, etc. In formula each R1 and R2 independently means H; C1-C10alkyl; halogen; CF3; CN; OR5; NR6R7; NHCOR8; aryl; or C1-C4alkyl optionally substituted by OR5; Z means OR3; R3 means C1-C6alkyl or C1-C3alkyl, substituted by C1-C3alkoxy group; R4 means SO2NR6R7; NR9R10, providing NR9R10 is other than NH2; COR11; OR12; or R4 means 5-6-merous heterocyclyl optionally substituted by one or more substitutes specified in a group consisting of OH and C1-C6 alkyl; or R4 means 5- or 6-merous cyclic monosaccharide group; R5 means C1-C6alkyl; C1-C4alkyl optionally substituted by C1-C4alkoxy group; each R6 and R7 independently means H, OH, C1-C6alkyl, C1-C6alkoxy group, C3-C6alkenyl, C3-C6cycloalkyl, adamantyl, C3-C8lactamyl, aryl, Het or (CH2CH2O)jH, wherein j is 1-3; or each R6 and R7 independently means C1-C6alkyl, optionally substituted by OH, C1-C4alkoxy group, SO3H, SO2NR13R14, SO2R16, NR13R14, aryl, Het or 5-6-merous heterocyclyl; or each R6 and R7 independently means 5-6-merous heterocyclyl optionally substituted by one or more substitutes specified in a group consisting of C1-C6 alkyl and C1-C6alkyl substituted by hydroxyl; or R6 and R7 together with a nitrogen atom attached whereto form 5-7-merous heterocyclyl optionally substituted by one or more substitutes specified in a group consisting of OH, COOR8, (CH2CH2O)jH, wherein j is 1-3, C1-C4alkoxy group, Het and C1-C6alkyl substituted by aryl; or R6 and R7 together with a nitrogen atom attached whereto form a glucosyl amino group, an amino acid residue, a residue of an amino acid ester or an amino amide residue. The other radical values are specified in the patent claim.
EFFECT: invention refers to pharmaceutical compositions based on the above compounds, using them, methods for preparing the compounds, and intermediate products.
18 cl, 2 tbl, 224 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: present invention refers to a formulation of a traditional Chinese medicine for treating bronchial asthma, and a method for preparing it. The traditional Chinese medical composition is prepared of pure herbal raw materials, including ephedra herb (Herba Ephedrae), ginkgo seeds (Semen Ginkgo), mulberry bark (Cortex Mori), skullcap root (Radix Scutellariae), bitter apricot seeds (Semen Armeniacae Amarum), pinellia rhinzomes (Rhizoma Pinelliae), perilla fruit (Fructus Perillae), foalfoot blossom (Flos Farfarae), red peony root (Radix Paeoniae Rubra), houttuynia herb (Herba Houttuyniae), trichosanthis root (Radix Trichosanthis), forsythia fruit (Fructus Forsythiae).
EFFECT: traditional Chinese medical composition is effective in treating bronchial asthma.
11 cl, 7 tbl, 12 ex
SUBSTANCE: inhalation formulation in the form of aerosol for treating bronchial asthma and chronic obstructive pulmonary disease containing ipratropium bromide monohydrate as an active ingredient, ethanol absolute as a solvent, an aerosol particle size regulator as an adjuvant consisting of triethyl citrate and an acid specified in a group: citric acid, hydrochloric acid, orthophosphoric acid, 1,1,1,4-tetrafluoroethane (HFA-134a) and/or 1,1,1,2,3,3,3-heptafluoropropane (HFA-227ea) as a propellant in certain proportions.
EFFECT: increasing the respirable fraction and obtaining an optimised particle size distribution profile.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine and pharmaceutical industry concerns a dry powder preparation containing formoterol fumarate dihydrate in the microionised form enabling bronchial and pulmonary alveolar penetration and additives, namely mixed lactose and sodium benzoate prepared in a particular way and enabling dosing and disaggregating the active ingredient effectively.
EFFECT: developing the preparation enabling dosing and disaggregating the active ingredient effectively.
4 cl, 8 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine, namely to pulmonology, and concerns a drug for treating inflammatory and obstructive respiratory diseases, particularly bronchial asthma and obstructive bronchitis, based on 1,3'-dinitroglycerine ester of 11(S),15(S)-dihydroxy-9-keto-5Z,13E-prostadiene acid, possessing a pronounced bronchodilatory effect. What is considered is a method of treating the inflammatory and obstructive respiratory diseases using the drug based on 1,3'-dinitroglycerine ester of 11(S),15(S)-dihydroxy-9-keto-5Z,13E-prostadiene acid.
EFFECT: invention provides extending the range of the broncholytic drugs ensured by using a natural prostaglandin E2 derivative characterized by higher biological activity and no observable side effects.
2 cl, 4 ex, 1 dwg
SUBSTANCE: invention refers to medicine, namely to pulmonology and may be used in treating the patients with chronic bronchitis in combination with secondary immunodeficiency. For this purpose, the patient with the remitting disease undergoes 6 procedures of ozone therapy every second days by the intravenous introduction of ozonised physiological saline with the ozone concentration 600 mcg/l in the ozone-oxygen gas mixture. One week after the ozone therapy, patient's venous blood is sampled and exposed to UV light for 2 procedures every 7 days. At the first procedure, the blood volume to be exposed makes 0.8 ml/kg of patient's body weight, and at the second procedure - 1 ml/kg.
EFFECT: method provides the effective treatment in the given category of patients that is ensured by the activation of the cell, phagocytic and humoral immunity without the use of aq drug-induced therapy as a result of the combined selected regimen.
2 tbl, 2 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to medicine and pharmaceutical industry and deals with solution of high-efficient composition in form of solution of active and additional substances, which makes it possible to form aerosol of small particles aimed at penetration in bronchi and pulmonary alveoli. Solution for obtaining medication for inhalational introduction contains fenoterol hydrobromide and ipratropium bromide as active substances, and sodium benzoate, food organic acid, purified water as additional substances with specified ratio of components.
EFFECT: solution has high value of respirable fractions.
6 cl, 3 ex
SUBSTANCE: invention refers to medicine, namely to cardiology and pulmonology, and concerns treating the patients with chronic obstructive pulmonary disease and ischemic heart disease and cardiac failure complicated by anaemia. For this purpose, pre-treatment haemoglobin and serum iron are evaluated. In that case, if the hemoglobin level is less than 110 mm/l, and the serum iron level is less than 5.85 mmol/l, the conventional treatment involving ACE inhibitors, beta blockers, diuretics, antiaggregants, statins as well as salmeterol and berodual, is added by prescribing an anti-anaemic therapy with Epocrin and Ferrum Lek. Epocrin is administered in a dose of 50 IU/kg subcutaneously 6 times a week; Ferrum Lek is administered in a dose of Fe 100 mg in 5 ml intravenously twice a week. The therapeutic course is 3 months.
EFFECT: this dose schedule of the preparations provides reducing a length of treatment by improving the central haemodynamics, and enhancing the respiratory function.
FIELD: medicine, pharmaceutics.
SUBSTANCE: there are presented: the use of aclidinium for preparing a medicine for treating or preventing a respiratory disease or condition in a patient by inhalation, wherein said patients showed no systemic antimuscarinic actions, where the patient suffers a condition or is susceptible thereto, which may be exacerbated by systemic antimuscarinic activity (versions), and a related method of treating or preventing.
EFFECT: invention provides local therapeutic action of aclidinium in lungs and has no significant systemic antimuscarinic action as it quickly hydrolysed in blood plasma, and its main metabolites are completely deprived of affinity to muscarinic receptors.
13 cl, 3 ex
SUBSTANCE: invention relates to novel isoquinolinone derivatives of formula (I) , wherein R1 is selected from H, (C1-C6)alkyl, (C2-C6)alkenyl, (CH2)a-X-Ar and (CR101R102)a-X-Ar, where said (C1-C6)alkyl is optionally substituted with 1, 2 or 3 groups independently selected from -(C1-C6)alkoxy, -halogen, -OH, -heterocycloalkyl, (C3-C7)cycloalkyl and -NR8R9; R2 is selected from H and (C1-C6)alkyl; R is selected from H, (C1-C6)alkyl and (CH2)d-Y; provided that when R3 is (CH2)d-Y, R2 is selected from H; R4 and R5 are independently selected from H, (C1-C6)alkyl and halogen; R is (C3-C7)cycloalkyl; R7 is H; Ar is phenyl or heteroaryl, optionally substituted with 1, 2 or 3 groups independently selected from -(C1-C6)alkyl, -(CH2)e-O-(C1-C6)alkyl, -(CH2)e-S(O)f(C1-C6)alkyl, -(CH2)e-N(R10)-(C1-C6)alkyl, -(CH2)e-Z-(C1-C6)alkyl, -halogen, heterocycloalkyl, -C(O)NR8R9, -NR8R9 and -C(O)OH, where (C1-C6)alkyl in each case is independently optionally substituted with 1, 2 or 3 groups, independently selected from -NRI2R13; X is selected from a single bond; Y is NR16R17, where R16 and R17 together with a nitrogen atom with which they are bonded form a 5-7-member ring, optionally containing an additional heteroatom NR27, where said ring is optionally substituted on the carbon atom with 1 or 2 substitutes independently selected from -(C1-C6)alkyl, where said -(C1-C6)alkyl is optionally substituted with -OH; and where R27 is selected from H and (C1-C6)alkyl, where said (C1-C6)alkyl is optionally substituted with -OH; Z is selected from C(O)N(R18); R8 and R9 are independently selected from H and (C1-C6)alkyl, where said (C1-C6)alkyl is optionally substituted with 1, 2 or 3 groups, independently selected from NR19R20; or R8 and R9 together with the nitrogen atom with which they are bonded form a 5-6-member ring, optionally containing an additional heteroatom, selected from NR21; R12 and R13 are independently selected from H and (C1-C6)alkyl, where said (C1-C6)alkyl is optionally substituted with -(C1-C6)alkoxy, -OH; or R12 and R13 together with the nitrogen atom with which they are bonded form a 5-6-member ring optionally containing an additional heteroatom selected from NR24; R10, R18, R19, R20, R21, R22, R23 and R24 are independently selected from H and (C1-C6)alkyl; a is selected from 1, 2, 3, 4, 5 and 6; d equals 0 or 1; e equals 0; f is independently selected from 1 and 2; where the heterocycloalkyl is a 5-6-member non-aromatic cyclic ring bonded at a C atom, having 1-2 NR28 atoms; optionally having one double bond; the heteroaryl is a 6-member aromatic ring containing 1 N atom; R is selected from H, (C1-C6)alkyl and -C(O)O-(C1-C6)alkyl; R101 is (C1-C6)alkyl; R102 is H; or pharmaceutically acceptable salts thereof or N-oxides. The invention also relates to methods of producing said compounds and use thereof as a p38 kinase inhibitor.
EFFECT: improved method.
13 cl, 4 dwg, 1 tbl, 128 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: present invention refers to biotechnology, more specifically to allergen modifications to reduce an allergenic capacity thereof, and may be used in medicine. A modified allergen is prepared by sequential modification of all primary amino groups or a portion thereof of lysine and arginine residues of an allergen molecule with using potassium cyanate and phenylglyoxal and used as an ingredient of a pharmaceutical composition for treating allergy.
EFFECT: invention provides preparing the modified allergen possessing the reduced allergenic capacity as compared to a respective native allergenic material and allergoids prepared by modification by either cyanate, or phenylglyoxal.
9 cl, 12 dwg, 4 ex
SUBSTANCE: monocytes are isolated from venous blood MNCs using the Percoll cushion bi-gradient of density: 47.5% SIP and 15% SIP, respectively, at cooling to +4°C. The monocytes are placed in a completely nutritional culture medium with adding 20 ng/ml IL-4 and 20 ng/ml GM-CSF. The completely nutritional culture medium is replaced on the day 3 of cultivation. On the day 4 of cultivation the antigen of infectious origin Opisthorchis felineus is added at a dose of 40 mcg/ml and maturation of dendritic cells is simultaneously induced with lipopolysaccharide E.coli of serotype 055: B5 in a dose of 1 mcg/ml. On the day 6 of cultivation the dendritic cells are washed and analysed.
EFFECT: use of the method provides obtaining the mature, antigen-loaded dendritic cells.
FIELD: medicine, pharmaceutics.
SUBSTANCE: inventions refers to biotechnology and concern hypoallergic fused proteins. A presented hypoallergic fused protein consists of one hypoallergic molecule originated from an allergen, fused with a second non-allergic protein originated from a pathogen, or a fragment thereof with the hypoallergic molecule originated from the allergen showing 50% decreased ability to bind IgE, 30% decreased T-cell responsiveness as compared with a wild-type allergen. A nucleic acid molecule coding the fused protein, an expression vector, a host cell expressing the same protein, and a vaccine composition containing the protein are also presented.
EFFECT: presented invention enables preparing therapeutic and prophylactic drugs for an allergy or diseases caused by pathogens with using no toxic adjuvants, showing no side effects.
14 cl, 23 dwg, 17 tbl, 27 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to pharmaceutics, namely to allergens purified from apitoxin and derived allergoids for immunotherapy of individuals suffering specific allergic reactions. The allergens for immunotherapy are prepared by dialysis of native apitoxin with using a membrane with cut-off 10 kDa, and represent apitoxin substantially mellitin-free. The apitoxin allergoids for immunotherapy prepared by carbamylation or thiocarbamylation or treatment to form guanidine groups of primary amino groups of protein portions of mellitin-depleted apitoxin. The pharmaceutical composition for immunotherapy on the basis of apitoxin which contains an effective immunotherapeutic dose of the allergen or allergoid together with pharmaceutically acceptable excipients. The use of the allergens or allergoids for preparing a drug preparation for immunotherapy.
EFFECT: agents described above are effective for immunotherapy.
15 cl, 6 dwg, 1 tbl, 2 ex
SUBSTANCE: invention refers to medicine, namely allergology, and may be used for treating atopic dermatitis. That is ensured by the introduction of a preparation of a complex of cause significant allergens is combined with using antiallergic antimediators and topic steroids. The procedures of allergovaccination are conducted once a day in dose 0.1 ml to be increased by 0.1 ml daily to 0.9 ml in the 10-6 potency of cause significant allergens to 1 ml of the undiluted complex of allergens introduced once every 2 weeks for 3 years. The allergovaccination by cause significant allergens is performed with underlying pharmacological remission (control) of atopic dermatitis by external steroids and systemic therapeutic antimediators.
EFFECT: method provides higher clinical effectiveness in atopic dermatitis ensured by improved pharmacological control of the disease during the staged allergen-specific immune therapy, as well as enables effective arrest of the manifestations of atopic dermatitis with multiple sensibilisation.
SUBSTANCE: invention refers to veterinary microbiology. A method for growing atypical mycobacteria cultures on a liquid nutrient medium, culture inactivation by autoclaving at 120°C for 30 minutes, protein settling from a culture filtrate of each strain separately by trichloroacetic acid to the final concentration of 4%, re-settling by ammonium sulphate at 50% saturation, protein dialysis through a cellophane coating as against deionised water to remove salts; analysis of the protein solution for its activity in each atypical mycobacteria culture, mixing of the protein solutions in equal portions by the "ED" concentration, sterilising filtration of the solution, packaging and lyophilisation. As atypical mycobacteria cultures, M. intracellulare, M. scrofulaceum, M. fortuitum and M. smegmatis are used, while the atypical mycobacteria cultures are grown on the liquid nutrient medium for 2-3 weeks.
EFFECT: method provides high-quality differential diagnostic response on PPD tuberculin for mammals and cutting economic loss of induced PPD tuberculin responded animal killing.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to pharmacology and medicine, namely to a method for producing diagnostic allergens of a number of synanthropic insects, such as: Blattella germanica, Blatta orientalis, Pertplaneta americana cockroach Neuphoeta cinerea, Musca domestica, Tinea pelionella, Cimex lectularis, Monomorium pharaonis, Tegenaria derhami, Culex pipiens, Attagenus Smirnovi Zhan. For the method for producing the diagnostic allergen, initial raw materials are frozen synanthropic insects. The initial raw materials are grinded to particle size 1-3 mm, degreased by addition of ether in the ratio 1:3-4 to the raw materials; the raw materials are kept in ether during 15-20 min that is followed by removal of ether by mixture filtering; the raw materials are degreased again by ether, dried to complete ether removal; the completely fat-free raw materials are powdered. The prepared powder is extracted in two stages; the first stage involves pouring the powder with an extractant liquid 1:1 and mixing to a homogeneous suspension; and the second stage includes mixing the prepared suspension with the extractant liquid in the ratio 2:1 and agitating by shaking the suspension three times for 30 minutes every 1.5 hours at temperature 2-10°C for 3 days with daily correction of the pH value of the allergenic extract to 7.0-7.2.
EFFECT: invention provides extended range of the diagnostic allergens and creation of a highly effective preparation for the diagnostic diseases caused by sensitisation to synanthropic insects.
2 cl, 1 ex, 1 tbl
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to field of biotechnology, and describes method of obtaining preparation for carrying out application skin test, which includes mixing tested substance with carrier and solvent, as carrier used is gar-agar, as solvent - borate buffer pH 6.8-7.2, agar-agar in borate buffer is heated with mixing to complete dissolution of agar-agar, mixture is cooled to 40-45°C and tested substance in amount 10-50% of total volume of ready preparation is introduced, as tested substances used are their water solutions with concentrations, capable of inducing contact reactions of hypersensitivity.
EFFECT: method makes it possible to detect hypersensitivity to tested substances.
SUBSTANCE: invention refers to medicine and concerns cat allergen fused proteins and application thereof. Substance of the invention involves a compositions containing a virus-like particle (VLP) or a viral particle and at least, one antigen, first of all at least, one cat antigen, and more specifically at least one cat antigen which is human allergen. In specific versions of the invention, said antigen represents cat antigen Fel d1 or its fragment covalently bound with the VLP.
EFFECT: invention can be applied for preparing vaccines first of all aimed at treatment and/or prevention of cat dander allergy and other cat antigens and allergens responses.
25 cl, 20 ex, 5 tbl, 4 dwg
FIELD: medicine, veterinary science.
SUBSTANCE: invention concerns veterinary medicine. Currently, for differentiating nonspecific tuberculin reactions in cattle, dried purified tuberculin is used for mammals and "КАМ" with considering the sensitisation pattern by the reaction intensity. A common complex allergen is produced by protein settling from M scrofulaceum No. 12-C and M intracellulare No. 13-H strain cultures with added allergen produced from Corynebacterium xerosis N1911, in amount 1350 units of activity. The presence of coryneformic bacteria allergen in the "КАМ" composition improves the efficacy of a simultaneous dried purified tuberculin test for mammals in differentiating the nonspecific coryneformic bacteria reactions.
EFFECT: use of the declared allergen allows to prevent unreasonable slaughter, as well as further diagnostic finding expenses.
SUBSTANCE: invention represents a pharmaceutical composition for treating demodectic blepharitis and blepharoconjunctivitis, containing recombinant interferon specified in a group: recombinant interferon alpha, recombinant interferon beta, recombinant interferon gamma, boric acid, fluconazole and/or voriconazole with the ingredients of the composition taken in certain ratio, g in 1 ml.
EFFECT: higher therapeutic effect.
2 cl, 6 ex