Method of obtaining collagen from biological material

FIELD: chemistry.

SUBSTANCE: invention represents a method of obtaining collagen from biological material, which includes milling of a raw material, liquid processing of the biological material with obtaining a collagen-containing substance, separated into a sediment and liquid fraction, characterised by the fact that as the biological material applied is a medusa, preferably Rhopilema, preferably its cupola, which is crushed preferably to 1-2 mm, and for obtaining the collagen-containing substance the material prepared in such a way is mixed with drinking water with a ratio by the raw material weight to water as 1:2 and extracted at a temperature preferably of 15-18°C for 6-12 hours with periodical mixing, after that, the obtained extract is separated into the liquid fraction and collagen-containing sediment, which is after that dehydrated to moisture weight in it not more than 10%, after which it is pre-packed and packed.

EFFECT: extension of an arsenal of methods for obtaining neutral collagen.


The invention relates to methods of producing collagen from biological material.

A method of obtaining collagen by grinding the biological material, processing naydennym solution of sodium sulfate containing 10% sodium hydroxide solution, with exposure times from 3 to 5 days, neutralizing the alkali aqueous solution of boric acid, washing with running water, dissolution of the treated material in odnomestnoi acetic acid and purification by dialysis against distilled water for 3 days (see Halkin A. M., Schechter A. B., Istrana L. P., Lemeni C. L. Collagen and its application in medicine, 1976. - M.: Medicine. - S. 47-50).

The disadvantage of this method is the long time required for separation of the finished product. In addition, to obtain collagen requires expensive reagents.

Also known is a method of obtaining collagen from biological material, comprising the grinding of raw materials, liquid handling biological material with obtaining collagen containing substance that is separated into sludge and the liquid fraction (see EN No. 2322249, 2008).

However, the use as solvent of acetic acid, causing acid reaction end product, and the presence of excessive content of acetic acid and its salts, high (20%) concentration of caustic is atra, long-term (within 24 hours) washing the treated material have the effect of restricting the application of the method. In addition, these methods are based on a relatively limited range of input sources.

The task of the invention is to provide opportunities for neutral collagen and the expansion of raw materials for its production.

The technical result - the possibility of obtaining neutral collagen and the expansion of raw materials for its production.

The problem is solved due to the fact that the method of producing collagen from biological material, comprising the grinding of raw materials, liquid handling biological material with obtaining collagen containing substance that is separated into sludge and a liquid fraction, characterized in that as the use of biological material jellyfish, preferably Rhopilema, preferably its dome, which is preferably crushed to 1-2 mm, to obtain collagen containing substance thus prepared material is mixed with potable water at a ratio by mass of the raw material to water as 1:2 and extracted within 6-12 hours at room temperature, after which the extract obtained separated into a liquid fraction and the precipitate, which is then dehydrated to the mass of moisture in it no more than 10%, after CEG is Packed and Packed. In addition, the liquid fraction of the extract used for the production of drinks.

Rhopilema asamushi is considered the most valuable of Medusa fished for food purposes 12 species scyphoid gellately. Currently, this jellyfish is implemented in Europe and South-East Asia, mainly in the form of salted-dried semi-finished processed various alum used as preservatives to increase the shelf life of the product and stabilizers of its structure. In Russia jellyfish as food is not widely used. Foreign methods of preparation and use of jellyfish in food are not acceptable for the Russian consumer due to the use of prohibited sanitary-hygienic standards for food additives in the manufacture of prefabricated jellyfish.

The basic way to handle jellyfish squad Rhizostomeae abroad [Goy J., Toulemont A. Meduses. - Monako: Musee oceanographique, 1997. - 160 p.] is the production of salted-dried products. Abroad dried and salted-dried products from jellyfish is in high demand and has a high retail price. Product eaten salted-dried as snacks with soy sauce, fried, added to salads and so on

Food facilities scyphoid jellyfish as important types of non-raw materials are the most valuable food products due to the prohibited content in them is well digestible and full of protein, biologically valuable fat, a variety of vitamins, minerals and biologically active components.

This is confirmed by histological and microstructural studies. Fabric jellyfish contains a large number of biologically valuable nucleic acids that increase the immunity of the human body. Protein jellyfish presents biologically and pharmaceutically valuable collagen - gelatinous protein, the main organic constituent of connective tissue and the organic component of bone, as evidenced by the effectiveness in the treatment of arthritis [Hsieh, Y-H. P., Leong F.-M. & J. Rudloe Jellyfish as food // Hydrobiology. 2001. V. 451. P. 11-17]. Between collagen and elastic fibers in the matrix of the mesoglea extracellular matrix are biologically active fibroblasts - the cells that produce collagen fibers in the places of their injuries.

Protein jellyfish well balanced amino acid composition, containing all the vital essential amino acids, as well as a wide set of free amino acids, which have beneficial effects on the metabolic processes in the body that characterizes the biological and nutritional value of products from the Pacific gellately.

Research fractional and fatty acid composition of lipids jellyfish suggests that lipids umbrella part and the mouth of the blades jellyfish which contain triglycerides, phospholipids, di - and monoglycerides, sterols, esters of sterols and free fatty acids.

In the fatty acid composition of the Pacific scyphoid jellyfish are saturated, monounsaturated and polyunsaturated fatty acids. The study of fatty acids found in covalently bound form in the lipid composition of different classes, suggests that lipids Pacific jellyfish have a higher biological value, as well as fatty acids 16-20% consist of a series of essential, necessary for normal functioning of the body polyunsaturated acids: linoleic, linolenic, arachidonic, eicosapentaenoic.

The mineral composition of jellyfish presents a wide range of macro - and micronutrients, predominant among which are iron, chlorine, iodine, sodium, chromium, aluminum, fluoride, manganese, and bromine.

The cost of jellyfish in Russia, below the cost of sea fishery (fish and valuable seafood).

Comparative analysis of the characteristics of the claimed solution with the characteristics of the prototype and analogues demonstrates compliance of the claimed solution to the criterion “novelty”.

The characteristics of the characterizing portion of the claims, solves the following functional tasks

Signs "as the use of biological material jellyfish" allow to draw for the production of new collagen is type of biological material, the resource which is virtually unlimited.

Signs indicating the use of Medusa preferably Rhopilema", enable the use of species of jellyfish, a significant period of use in food which is in the Eastern cooking confirmed its edibility.

Signs indicating the use of a preferably dome jellyfish, provide the possibility of involvement in the trafficking of waste jellyfish, because in the East the kitchen is used preferably tentacles.

Signs indicating that the dome is ground preferably 1-2 mm, enable rapid extraction of collagen containing materials from the crushed material by increasing the specific surface area of its particles.

Signs indicating that to obtain a collagen containing substance chopped biomaterial "is mixed with potable water at a ratio by mass of the raw material to water as 1:2 and extracted within 6-12 hours at room temperature, simplify the process of biomaterial, avoid the use of reagents and provide for effective disposal of liquid wastes from this process (due to the use for the manufacture of beverages).

Signs indicating that "the extract obtained is separated into a liquid fraction and a residue will cause separation of the extract on the departure of the (liquid fraction) and the intermediate product, containing collagen.

Signs indicating that sediment "then dehydrate until the mass of moisture in it no more than 10%", ensure the implementation of the last stage of obtaining collagen.

The signs are that the finished product is Packed and Packed"to provide the "giving" product presentation.

Signs of the second claim of the invention provide for the disposal of liquid waste production of collagen to get a useful product.

For making use of collagen jellyfish raw (TU 9250-327-00472012-09) or frozen jellyfish (TU 9265-330-00472012-2010).

The raw materials used and materials on hygiene requirements food safety must comply with the Uniform sanitary and epidemiological and hygienic requirements for goods subject to sanitary and epidemiological supervision (control), and be accompanied by documents confirming its quality.

Water used for technological purposes, must comply with SanPiN "Drinking water. Hygienic requirements to water quality of centralized drinking water supply systems. Quality control".

Allowed to use the marine clean water that meets the requirements of SanPiN

All technological equipment used in the manufacture of collagen, must match the Tr is the requirements SanPiN "enterprises of food and processing industry (technological processes, raw materials). Production and sale of fish products. Sanitary rules and norms".

Reception of raw materials, storage until processing is carried out in accordance with the requirements of normative documents.

Received for processing jellyfish raw washed of impurities clean flowing seawater or potable water to a temperature not higher than 15°C. the Washed jellyfish sent for cutting. Cutting jellyfish carried out manually, as follows: jellyfish cut off the dome and rapalee (tentacles). The dome jellyfish make an incision lengthwise and remove the innards. The large dome is cut into pieces no larger than 5×5×7 see the Dome and/or pieces of the dome and rapalee sent for washing. The dome and/or pieces of the dome, rapalee separately each type of raw material is placed in a wooden or plastic container lined with plastic wrap, and washed 2-3 times from contamination by a stream of drinking water, allowed to use clean sea water temperatures ranging from 15 to 20°C. After draining water, the product is sent for crushing.

Frozen jellyfish thawed to a temperature of from 0 to -2°C within a block of the material after washing sent for cutting, as above.

Raw materials (including thawed) are ground in an electric grinder with a hole diameter of 1-2 mm can be used homogenizers and devices thin ismel the treatment of materials (a & m) according to their instructions.

To the crushed raw materials add drinking water in the ratio 1:2, the mixture was mixed thoroughly and leave for extraction at a temperature of 15-18°C for 6-12 hours, while periodically stirring.

Upon completion of the extraction step carry out the separation of the extract, which is divided into the sediment and the liquid part using the separator of known construction, for example I-as-2ZH when the rotational speed of 6500 rpm according to his instructions.

At the completion of phase separation carry out the drying and grinding of the resulting sludge. The process is produced in a known manner, a vacuum drying apparatus of the drum type. The drying process begins with the heating of the vacuum pot within 30 minutes the Temperature is 70°C. Then loads the received material into the apparatus in the amount of 20 kg Sunroof boiler seal, include a vacuum pump (residual pressure of 2 kPa). The temperature inside the boiler is 35°C. Include rotation of the boiler, the angle of rotation should not exceed 360°. After 3.5 h of drying stops the rotation of the boiler, relieve the vacuum in the boiler load grinding media for grinding product. Drying continued for 2.5 h with the same parameters. Then the temperature was lowered to 50°C and continue the drying process even 2,0 hours Then the rotation of the boiler remains the pour, relieve the vacuum, open the hatch of the boiler, in its Central part enter the sieve and the collection of the finished product. The hatch closed, the boiler is again put into rotation. Stop the boiler is produced after the cessation of the shedding of the powder from the walls of the device.

Use of freeze-drying according to the operating instructions of the installation that implements this process.

The finished product with a mass fraction of moisture not more than 10% is directed to filling and packaging.

Packaged products according to GOST 7630-96 in packages made of polymer materials OST 15-390-2005 maximum mass of 1.0 kg, followed by packing in boxes made of corrugated cardboard on the EAST 15-409-2002, GOST 13511-2006, GOST 13516-86 maximum mass of 20.0 kg Boxes glued adhesive tape on the paper base according to GOST 18251-87 or plastic tape with an adhesive layer according to GOST 20477-86.

Packaging products in bags made from polymer materials made in accordance with packing Instruction food fish products in bags and liners from plastic materials".

Containers and packaging materials used for packaging must be clean, solid, dry, free from foreign smell and made of materials permitted by the authorized bodies for contact with this type of product.

Collagen is stored at a temperature not above 20°C and relative to the air humidity not more than 80%, protecting from the sun's rays. The shelf life of the products with the manufacturing date is 24 months.

The proposed method of producing collagen economical, does not require the use of reagents. The final product has a neutral reaction and purified from salts and other impurities, which determines the possibility of its widespread use.

The liquid fraction obtained at the end of phase separation of the extract used for making drinks, preferably isotonic, for supplementing mineral-salt balance of the body of people working in difficult conditions or athletes, for example, mixing it with clean drinking water, sweeteners, poikilitically etc. ingredients.

A method of producing collagen from biological material, comprising the grinding of raw materials, liquid handling biological material with obtaining collagen containing substance that is separated into sludge and a liquid fraction, wherein the biological material used Medusa, preferably Rhopilema, preferably its dome, which is preferably crushed to 1-2 mm, to obtain collagen containing substance thus prepared material is mixed with potable water at a ratio by mass of the raw material to water as 1:2 and extracted at a temperature of preferably 15-18°C in t is the within 6-12 hours with periodic mixing, then the extract obtained is separated into a liquid fraction and a residue containing collagen, which is then dehydrated to the mass of moisture in it no more than 10%, then Packed and Packed.


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