Method of determining degree of inflammatory process expression in case of knee joint osteoarthrosis
SUBSTANCE: invention relates to medicine, namely to method of determining expression of inflammatory process in case of osteoarthrosis. Essence of method consists in the following: carried out is luminol-dependent iron-induced chemi-luminescence of model system, which has the following composition: 2.72 g of KH2PO4, 7.82 g of KCl, 1.5 g of sodium citrate C6H8O7Na3*5,5H2O per 1 liter of distilled water, pH 7.45 with 0.2 ml of 10-5 M luminol solution, after that, intensity of model system luminescence is determined in presence of synovial fluid before and after addition of synovial fluid. Degree of suppression of intensity of model system chemi-luminescence is calculated by formula. If its value is from 1.71 to 6.48%, high activity of inflammatory process is determined, if its value is from 6.49 to 21.55%, medium activity is determined, from 21.56 to 55.46 - small activity.
EFFECT: application of the method makes it possible to reduce time of determination and increases accuracy of assessment of inflammatory process degree in case of osteoarthrosis.
1 tbl, 1 dwg, 3 ex
The invention relates to medicine, namely to traumatology, orthopedics and rheumatology.
Osteoarthritis is the main place in the structure of the musculoskeletal system. In Russia, the average prevalence of osteoarthritis of the knee is 6.43% and reaches to 13.6% in persons older than 45 years [Ardis W. F. Prevalence of articular syndrome in Russia / W. F. Ardis, E. A. Galushko, D. I. Bazorkina, T. Y. Bolshakov // Scientific and practical rheumatology: proc. Dokl. IV Congress of rheumatology in Russia. - Kazan, 2005. - S. 146]. Diseases of the musculoskeletal system recognized socially aggravating factor, as are the second duration and the third cases of temporary disability among all classes of diseases. Osteoarthritis of the knee is the most frequent cause of chronic joint pain, and it largely determines the medical and social importance of the issue under consideration. However, early diagnosis of osteoarthritis is difficult, despite numerous studies in this field.
There is a method of determining the severity of the pathological process in osteoarthroses through the study of biochemical parameters of blood: rumorosa, antistreptolysin and C-reactive protein [Korshunov N. And., Marasai centuries, Osteoartroz // Gonarthrosis: Instrumental non-invasive methods of diagnostics is Tiki. - Yaroslavl, 2005. - S. 22-23]. However, biochemical methods usually detect pathological changes to severe stages of the disease.
It is known that in inflammatory diseases of different etiology in the blood change processes of free-radical oxidation and antioxidant activity. However, free-radical oxidation and antioxidant activity in the blood is affected by various diseases, so this indicator is not strictly specific in osteoarthritis.
The closest analogue of the invention is a method of evaluation of anti-inflammatory activity of synovial fluid in diseases of the large joints, which consists in the fact that conducting a study of chemiluminescence synovial fluid, thus determine its content of protein, then in the cuvette for chemiluminescent studies contribute intact neutrophils of healthy donors, obtained from lacokanostra, count the number of and parallel to another cell for chemiluminescent studies contribute intact neutrophils donors (NF) and synovial fluid of the patient, record the chemiluminescent response of cells of the test system, calculate the index of the chemiluminescent response (Andholdem, pulse/NF/g protein) according to the formula
The disadvantages of this method are the need for neutrophil donor, counting their number - indicators, which are not standardized and each time is different, as well as the determination of protein in synovial fluid of a patient, which increases the duration of the study.
The objective of the invention is to improve the efficiency of diagnosis of the severity of the inflammatory process in osteoarthritis by the study of the antioxidant activity of synovial fluid in the knee joint of the patient.
The technical result when using the invention, reducing the time and improving the accuracy of estimates of the extent of the inflammatory process in osteoarthritis of the knee.
The proposed method is as follows.
Synovial fluid taken by syringe after puncture the top of the inversion of the knee joint (recessus suprapatellaris) at the lateral edge of the base of the patella. The needle is moving perpendicular to the axis of the thigh under the tendon stretching of the quadriceps muscle to a depth of 3-4 cm and selected content. Preparing a model system of the following composition: 2,72, KH2PO4, of 7.82 g of KCL, and 1.5 g of sodium citrate C6H8O7Na3*5,5 H2O in 1 liter of distilled water. The pH of the resulting solution was adjusted to 7.45% by titration with saturated solution of KOH and add 0.2 ml of 10-5M solution lyuminola. In the cell of chemiluminometer paid 10 ml of the resulting system of standard composition, which initiate the formation of active forms of oxygen by the introduction of 1 ml of 50 mm solution of iron sulfate (FeSO4). When this develops chemiluminescence - glow accompanying the formation of free radicals. Determine its intensity for 5 min measurement. Then measure the chemiluminescence model system, which is added to 0.5 ml of the obtained patient's synovial fluid. P is the degree of inhibition of chemiluminescence model system in the presence of synovial fluid is determined by its antioxidant activity, which corresponds to the severity of the pathological process and is calculated by the formula:
where Imodthe intensity of the model system, I+SYN.fluidthe intensity of the model system after adding synovial fluid.
When the value of the degree of suppression of the intensity of chemiluminescence model system 1.71 to 6,48% define high activity of the inflammatory process, from of 6.49 to 21.55% - average activity, from 21,56 to 55,46% of small activity. The whole registration process chemiluminescence and processing results are in automatic mode, which allows to increase the accuracy and objectivity of the information received. The duration of the measurement is 5 minutes.
The invention is illustrated in the drawing, which shows the recording chemiluminescence, where 1 is a typical entry chemiluminescence model system in which adding iron salts are formed reactive oxygen species: the process is accompanied by a quick flash followed by a latent period, which goes into slow flash; 2 - after adding the synovial fluid. As the most informative measure is used as the chemiluminescence intensity during the measurement. Adding to the model system of synovial fluid inhibits chemilum is ascencio.
Under our supervision in the municipal unitary enterprise "Center of manual therapy" ,Ufa were 20 patients with osteoarthritis of the knee in age from 46 to 66 years, men and 2 women - 18. Duration of disease ranged from 1 month to 1 year. To assess the severity of the disease was determined orthopedic status that includes clinical, biochemical, instrumental, sonography, x-ray and magnetic resonance tomographic studies of the knee. The antioxidant activity of synovial fluid was determined by the degree of suppression of chemiluminescence model system, generating reactive oxygen species (table). All patients were divided into 3 groups. group 1 - patients with significant improvement after treatment, which was set low activity of the inflammatory process, group 2 - improvement with medium activity, group 3 - a slight improvement with high activity.
For the treatment of patients used non-steroidal anti-inflammatory drugs, drugs, improves microcirculation, protective agents, physiotherapy, exercise therapy, hydrokinesitherapy, massage. Intra-articular injected glucocorticosteroid drugs (betamethasone sodium phosphate, betamethasone dipropionate) intervals through the day. The total number of injections ranged from 2 to 3 treatments. After copyrow is of synovitis weekly was introduced inside the joint "Hyalgan Fidia" from 1 to 3 injections per course of treatment.
All patients (n=20) in the process of treatment, the degree of suppression of chemiluminescence model system adding synovial fluid is increased, which indicated the increase of its antioxidant activity.
Clinical example 1
The patient 3. 1947 R., appealed to the municipal unitary enterprise "WTC" with complaints of pain in the left knee joint, limitation of movements.
Status localis: During the inspection region of the left knee revealed swelling, palpation in the projection of the joint space - pain. Motion limited. Bend the left knee joint 180-75 degrees, there limp on the left leg. While the survey found: complete blood count (ESR 48 mm/hour), ultrasound (in the cavities of the left knee moderate amount of inhomogeneous fluids, predominantly in the upper inversion and lateral inversions, articular gap narrowed) and x-ray of the left knee (signs of deforming arthrosis of the I century).
Diagnosis: Osteoarthritis of the left knee of the I century Synovitis. A survey of the proposed method. Imod- 42,27, I+SYN.- 20,22 degree of suppression of the intensity of chemiluminescence model system was 52,16, which indicates a low activity of the inflammatory process.
After out-patient treatment no complaints, WM is avnoj syndrome was arrested.
Status localis: When viewed from the left knee joint swelling was not detected, palpation in the projection of the joint space painless. Bend the left knee joint 180-125 degrees. Walking time restored.
The study of the antioxidant activity of synovial fluid in the knee joints showed an increase. Significant improvement of the condition, the severity of the inflammatory process is minimal.
Clinical example 2
Patient S., 1966 R., appealed to the municipal unitary enterprise "WTC" with complaints of pain in the knee joints, limitation of movement and discomfort.
Status localis: Upon examination of the knee joints revealed swelling, contours smoothed palpation in the projection of the joint space soreness. Motion limited. Bend the left knee joint 180-75 degrees. The bend in the right knee joint 180-80 degrees, there limp on the right leg.
While the survey found: complete blood count (erythrocyte sedimentation rate of 19 mm/hour), radiography of the knee joints (signs of deforming arthrosis II century).
Diagnosis: Bilateral gonarthrosis II senior Synovitis.
A survey of the proposed method. Imod- 14,64, I+SYN.fluid- 12,30 degree of suppression of the intensity of chemiluminescence model system was 15,98, which indicates the average activity populationincrease.
After out-patient treatment no complaints, articular syndrome is under control.
Status localis: When viewed in the area of knee joints swelling is not revealed, palpation in the projection of the joint space painless. Flex at the knee joints 180-120 degrees. Walking time restored. During the treatment the antioxidant activity of synovial fluid increased, the intensity of the inflammatory process is decreased, the marked improvement of the patient.
Clinical example 3
Patient O., 1951 R., appealed to the municipal unitary enterprise "WTC" with complaints of pain in the right knee joint, limitation of movements.
Status localis: When inspecting the area of the right knee revealed swelling, palpation in the projection of the joint space and the displacement of the patella, pain. Motion limited. The bend in the right knee joint 180-80 degrees, there limp on the right leg.
While the survey found: complete blood count (erythrocyte sedimentation rate of 17 mm/h), ultrasound examination of the right knee joint (determined thinning of the cartilage surface, the phenomenon of synovitis, degenerative-dystrophic changes in the anterior horn of the medial meniscus. Echo signs of gonarthrosis Art. II, synovitis).
Diagnosis: Octoarts right knee II senior Synovitis.
A survey of the proposed method. Imod- 7,84, I+SYN.- 26,69 degree of suppression of the intensity of chemiluminescence model system was 4,13, which indicates a high activity of the inflammatory process.
After out-patient treatment no complaints, pain syndrome was arrested.
Status localis: When viewed in the right knee joint swelling was not detected, palpation in the projection of the joint space painless. The bend in the right knee joint 180-115 degrees.
The patient's condition has improved slightly. The antioxidant activity of synovial fluid has not changed.
The method of determining the severity of the inflammatory process in the osteartrozis, including chemiluminescent study using synovial fluid, characterized in that conduct Lomonosovsky gelatination the chemiluminescence model system, which has the following composition: 2,72 g KH2PO4, of 7.82 g of KCL, and 1.5 g of sodium citrate C6H8O7Na3*5,5 H2O in 1 liter distilled water, pH was 7.45 with 0.2 ml of 10-5M solution lyuminola, then in the presence of synovial fluid determine the intensity of the glow model system before and after adding the synovial fluid, calculate the degree of suppression of the intensity of chemiluminescence model system by the formula:
Imodthe intensity of the model system,
I+SYN.fluidthe intensity of the model system after adding synovial fluid,
and when it is 1.71 to 6,48% define high activity of the inflammatory process, from of 6.49 to 21.55% - average activity, from 21,56 to 55,46% of small activity.
SUBSTANCE: diagnostic test element (110) for the detection of an analyte in a sample (126) of a body fluid, in particular whole blood with the volume not less than 2 microlitres, contains a test field (116) with a reagent-indicator, where the reagent-indicator is capable of feeling a detected change, in particular an optic change, in case of the analyte presence. The test field (116) includes a detector layer (118), containing the reagent-indicator, where the detector layer contains particles (137). 90% of all particles (137) of the indicator layer (118) have the actual size smaller than 10 microns. The diagnostic test element (110) contains a bearing element (112), which has a transparent region (114), where the test field (116) with its detection side (120) is partially applied on the transparent region (114).
EFFECT: detected change is an optically detectable change, where an optic detector with spatial resolution is applied for the detection of the detected change.
9 cl, 8 dwg, 1 tbl, 2 ex
SUBSTANCE: invention relates to immunology and medical diagnostics and represents method of carrying out immunochromatographic analysis for serodiagnostics. Claimed invention is intended for immunochromatographic determination of antibodies to causative agents of infectious diseases or other antigens, for instance, allergens, in liquid samples. Claimed method of identifying antibodies is characterised by the following: solution for sample dilution contains specific antibodies against test strip of antigen (or antigens) immobilised in analytic zone. Used concentration of antibodies in diluting solution is lower than lower limit of determination of antibodies by said method with application of diluting solution, which does not contain specific antibodies, which results coloration of analytic zone of test strip is not observed if specific antibodies are absent in sample. If sample contains specific antibodies, presence of additional quantity of specific antibodies in diluting solution results in enhancement of intensity of coloration of test strip analytic zone.
EFFECT: application of invention makes it possible to register total concentration of specific antibodies in sample and in diluting solution, which results in reduction of limit of analysis identification due to displacement of working range of concentrations, determined by test, into the area of lower values.
3 dwg, 1 ex
SUBSTANCE: invention refers to medicine and represents a method for accelerated staining of histological specimen for identifying tuberculosis mycobacteria involving the preparation of the histological specimen according to a common technique, the Ziehl-Nelsen staining differing by the fact that the standard preparation of the specimen for staining is followed by a de-waxing procedure by placing them successfully into xylol and alcohols of the decreasing concentration for 5 minutes in a thermostat at 54°C and then placing into water, then in a 1% periodic acid for 2 minutes and washing in flowing water for 10 seconds; thereafter, Ziehl carbol-fuxine is poured on a section coated with a filter paper and heated on an alcohol burner lamp until evaporating for 2 minutes; the stain is left on the section after the heating procedure is completed, for 3-5 minutes; the filter paper is removed, and the sections are washed in water, differentiated in a 1% acid buffer, washed in main water for 1 minute; thereafter Leffler methylene blue is placed on the section and heated on the alcohol burner lamp until evaporating, washed in water, differentiated in a 1% acid buffer and washed with a plenty of water, de-watered in alcohols of the increasing concentration for 1 minute, placed in xylol and balm.
EFFECT: developing the method for the accelerated stain of the histological specimen.
SUBSTANCE: invention refers to medicine and aims at the prediction of postoperative suppurative complications in patients suffering from colorectal cancer by a dynamic blood test. On the 2nd and 5th postoperative day, blood plasma of the patient suffering from colorectal cancer is examined for C-reactive protein (CRP). If the 5th-day CRP level is less than 10.0 mg/dl, a relatively mild postoperative course is predicted. If the CRP level decreases in relation to the second postoperative day, a risk of the complications is considered to be low. If the CRP level increases in relation to the second postoperative day or remains unchanged, a risk of the complications is moderate. If the 5th-day CRP level is more than 10.0 mg/dl, a risk of the postoperative suppurative complications is considered to be high.
EFFECT: invention provides the effective prediction of the suppurative complications in the patients on the second and fifth day following colorectal cancer surgery.
SUBSTANCE: ovarian tumour tissue is examined for a portion of the synthesis cycling-state cell; if the derived value is less than 15.9%, a favourable outcome is predicted, while at the derived value being more than 15.9%, an unfavourable outcome is predicted. The invention can be used more than once in clinics for women and special oncological hospitals for the purpose of predicting recurrent ovarian carcinoma according to the tumour tissue DNA-cytometry findings.
EFFECT: higher prediction accuracy.
SUBSTANCE: technique involves preparing a patient's blood serum sample by drying blood serum, milling the dry deposition and suspending in Vaseline oil. The prepared sample is analysed by IR-spectroscopy by determining a peak height of absorption bands with maxima 1165; 1160; 1150; 1100; 1070; 1050, and 1025 cm-1. And the peak height relations are calculated: the relation of the peak height with maximum 1160 cm-1 to the peak height with maximum 1165 cm-1; the relation of the peak height with maximum 1165 cm-1 to the peak height with maximum 1070 cm-1; the relation of the peak height with maximum 1165 cm-1 to the peak height with maximum 1150 cm-1, the relation of the peak height with maximum 1165 cm-1 to the peak height with maximum 1050 cm-1, the relation of the peak height with maximum 1100 cm-1 to the peak height with maximum 1050 cm-1, the relation of the peak height with maximum 1025 cm-1 to the peak height with maximum 1165 cm-1. The derived values are used to draw a differential diagnostic profile of the patient's blood serum sample to produce a plane polygon formed by 6 beams corresponding to 6 relations and to compare the above polygon to a polygon that is a reference differential diagnostic profile of pulmonary thromboembolism, wherein 6 relations make: 1 (1.85±0.26), 2 (0.28±0.13), 3 (0.39±0.07), 4 (0.25±0.13), 5 (0.58±0.10), 6 (4.47±1.70). If the derived patient's differential diagnostic profile has a similarity with the reference profile, and all 6 relations of the patient's blood serum sample coincide with the reference relations, pulmonary thromboembolism is diagnosed in the patient.
EFFECT: invention possesses high accuracy, easy to implement, and requires no heavy material and time expenses; it enables diagnosing thromboembolism of all pulmonary arteries both in the presence of clinically significant symptoms of the disease, and in the absence thereof.
2 ex, 3 dwg
SUBSTANCE: summary of declared method consists in determining the anticholinesterase effect of solutions of the examined anticholinesterase compounds (AnCEC) of a preparation of cholinesterase - the enzyme propionyl cholinesterase of calamari brain tissue (PCE), namely in estimating a percentage PCE activity inhibition in solutions of low (0.04 U/ml) and high (4 U/ml) concentrations. A toxicity hazard of the examined AnCEC solutions is assessed by a difference of their effect on PCE activity in the solutions of low and high concentrations. If a percentage of PCE activity inhibition in the solution of high concentration goes to zero or does not exceed 5-10%, the examined solution is stated to contain high-toxicity AnCEC of the 1st hazard class. If the inhibitory effect size remains constant or falls by no more than 10%, the presence of low-toxicity AnCEC is stated.
EFFECT: using the declared method enables assessing the toxicity hazard of the anticholinesterase compounds for 30-60 minutes in various situations, where their composition is unknown or they present in the form of solutions of an unknown concentration.
1 tbl, 2 ex
SUBSTANCE: invention relates to monitoring of the environment and biological objects aimed at identification of the content of metal ions in liquid media with the application of photochromic compounds. In the method of spectrophotometric identification of metal ions in liquid media with an application of photochromic compounds of class of chromenes due to formation of complexes between the photoinduced merocyanine form of the said compounds and metal ions as chromenes used are such bischromenes as: 2,2,11,11-tetrakis(4-methoxyphenylphenyl)dioxa(1,12)triphenylene, 2,2,8,8-tetrakis(4-methoxyphenyl)dioxa(1,7)chrysene, 3,3,11,11-tetra-(4-methoxyphenyl)-3,11-dihydro-4,10-dioxadibenzo[a,h]anthracene, 3,3,10,10-tetra-(4-methoxyphenyl)-3,10-dihydro-411-dioxadibenzo[a,h]anthracene.
EFFECT: increased selectivity of identification is achieved.
24 ex, 1 tbl, 6 dwg
SUBSTANCE: invention refers to medicine, namely to oncology, and can be used for the prediction of integrated clinical effectiveness in the patients suffering from lung cancer in an early postoperative period. Substance of the invention consists in the fact that a patient's blood plasma α-1-proteinase inhibitor is measured for the first postoperative day and perioperative autochemoimmunotherapy. If observing an increase of the derived value by 28% and more as related to the common norm, the treatment is predicted to be effective, while a variation of less than 28%, no variations, or a decrease as compared to the norm show ineffective treatment. A specificity of the method for the instant prediction of integrated clinical effectiveness at the initial stages is 80.9%, and for the prediction of no effectiveness - 97%.
EFFECT: using the method provides high specificity, enabling instant objective evaluation of integrated clinical effectiveness at the initial stages, carrying out cito analysis, as well as prescribing well-timed adequate therapeutic measures or replacing the treatment.
SUBSTANCE: invention is intended for more complete assessment of the oxidative modification of proteins (OMP) and analysis of the ratio of the aldehyde dinitrophenylhydrazones (ADNPH) and ketone-dinitrophenylhydrazones (KDNPH) of the basic and neutral character in plasma and blood cells as well as in animal tissues to determine the degree of manifestation and the stage of oxidative stress. The method is based on the fact that the OMP spectrum is divided into individual geometric shapes that represent rectangular trapeze. Area under the curve of the spectrum of oxidative modification of proteins consists of areas under the curve of ADNPH and KDNPH of the basic and neutral character and is determined by the formula.
EFFECT: method enables not only to estimate the total value of OMP, to determine the value of ADNPH and KDNPH of the basic and neutral character, but also to compare primary and secondary markers of OMP and thereby to reveal the path of violating the native conformation of proteins.
3 dwg, 2 ex
SUBSTANCE: invention relates to the field of biochemistry. Claimed is a method of evaluating cell viability in a microbioreactor by means of an optical light guide. The method includes the placement of cells into a membrane compartment of a replaceable cell unit of the microbioreactor, application of a working solution of a vital dye, introduction of the dye into the microbioreactor compartment. After the introduction incubation of the cells in the vital dye solution and removal of the vital dye solution, which has not bound with the cells, are performed. Removal is performed by the replacement of the incubation solution with a growth medium, which does not contain dye. The optical light guide, connected to a spectrometer, is brought into contact with an optically transparent material with the replaceable cell unit under the membrane compartment of the microbioreactor. After that, the support spectrum of a fluorescent signal is measured as an integral of the intensity of fluorescence on the membrane compartment of the microbioreactor, in which the cells to be analysed are absent. Also measured is the spectrum of the fluorescent signal as the integral of the intensity of fluorescence on the membrane compartment of the microbioreactor with the analysed cells. After that, the support spectrum of the fluorescent signal for the membrane compartment of the microbioreactor without the cells to be analysed is subtracted from the obtained spectrum of the fluorescent signal for the membrane compartment with the analysed cells. The quantity of the viable cells in the membrane compartment of the microbioreactor is calculated on the basis of the obtained value of the fluorescence signal intensity.
EFFECT: invention makes it possible to quickly determine viability of the cells under an impact of influencing factors in a real time mode in the microbioreactor.
6 cl, 3 dwg, 5 tbl, 5 ex
SUBSTANCE: invention refers to forensic medicine and biochemistry, and can be used to assign a cause of death conditioned by endointoxication syndrome. Substance of the invention: measuring average molecular weight peptides (AMWP) by an average molecule substance modified test in biological liquids of a dead body's vitreous body and blood serum. If the vitreous body AMWP is more than 0.50 g/l, and the blood serum AMWP is more than 2.8 g/l, endointoxication syndrome is diagnosed.
EFFECT: using the technique provides the higher accuracy and reliability, a possibility to diagnose over the prolonged postmortal period.
SUBSTANCE: invention refers to medicine, and represents a method for the prediction of a risk of congenital infections by measuring specific Ig M and Ig G antibodies in a biological material, differing by the fact that the biological material is presented by the first-screening cervical smear at the 12th week of gestation; the smears are tested for the IgG antibodies to the rubella virus, cytomegalovirus, B19V parvovirus, toxoplasm viruses, type 1 and 2 herpes simplex viruses and an avidity of the specific Ig G to this agents; additionally, the same smear is tested for secretory non-specific Ig A by IFA to cytomegalovirus, Chlamydia, Mycoplasm antigens, and a genetic material of this microorganisms by PCR, and depending on the findings, groups of a high, moderate and low risk of congenital infections are predicted.
EFFECT: invention provides the more accurate prediction of the risk of the most actual congenital infections by the integral assessment of a collection of clinical anamnestic data, and the qualitative parameters of the laboratory findings at the first pregnancy screening.
SUBSTANCE: invention refers to medicine. Substance of the method for the prediction of a probability of a restenosis progression taking into account a stent localisation in the right coronary artery and the circumflex artery consists in the patient's blood sampling at the moment of stenting and recording a prothrombin ratio, an atherogenic index, very little density lipoproteins, high-density lipoproteins in physical values, and a stenosis value S is calculated. That is followed by calculating a probability factor of the restenosis R progression according to the predicted restenosis value 6 months later by formula. An adequacy of the restenosis prediction value 6 months after stenting is provided for the following intervals: if the stenosis is localised in the circumflex artery, 0<R<40; if the stenosis is localised in the obtuse marginal artery, 0<R<50, 90<R<100; if the stenosis is localised in the right coronary artery, 0<R<50; if the stenosis is localised in the anterior interventricular artery, 0<R<100.
EFFECT: using the declared method enables the early prediction of recurrent cardiovascular complications, particularly the restenosis progression, recurrent cardiovasoconstriction following the stenting in the right coronary artery, the circumflex artery, the anterior interventricular artery, and the obtuse marginal artery.
SUBSTANCE: invention refers to medicine, namely to immunology, and can be used to assess the exposure of artificial light on the innate immune factors. That is ensured by assessing the exposure of light generated by light-emitting diodes, incandescent lamps on the neutrophil granulocytes recovered from a donor's peripheral blood. The neutrophil granulocytes are in vitro exposed to light in the light temperature range of 4000 K with solar radiation of 0.03 W/m2 at a wave length of 320 to 400 nm for various periods of time intervals. That is combined with stating the presence or absence of the following variations, such as phagocytic activity increase, lysosomal activity increase, biocidal abilities decrease recorded for the neutropils in the nitro blue tetrazolium reduction test.
EFFECT: using the given method enables assessing the biological safety of the new artificial light sources integrated into an individual's light-colour living environment with using a cell culture of the innate immunity and avoiding direct human participation.
SUBSTANCE: invention refers to laboratory diagnostics and can be used for testing biological samples for pathogenic microorganisms. The device for microbiological analysis of the body fluid samples comprises a compartment for incubating sample containers, an analyser for studying the internal atmosphere of the above containers and a sorting system for classifying the above containers in accordance with the carbon dioxide content detected by the above analyser. The sorting system classifies the containers delivered from the above incubation compartment to divide the above containers into the positive containers to be analysed for identifying microorganisms found in the respective samples, and the negative containers wherein the analysis is not required for identifying the microorganisms, and also the unspecified containers to be incubated once again.
EFFECT: invention provides simplifying and accelerating the analysis of the biological samples for the pathogenic microorganisms.
10 cl, 8 dwg
SUBSTANCE: clinical-laboratory and functional methods are used to determine diagnostic criteria and to calculate a prognostic index. A complex of the following diagnostic criteria is determined in a patient: anthropometric - a Quetelet index, a lipid metabolism - an atherogenic index of apoprotein, a functional hepatic state - alanine aminotransferase, lipid peroxidation - a relation of malondialdehyde to the antioxidant activity, central haemodynamic values - a systolic arterial blood pressure and cardiac index. A predictive index determining a degree of manifestation of metabolic disorders is the prognostic index as the total value of the diagnostic criteria determined by clinical-laboratory and functional methods and calculated by formula. The value derived from formula is used to predict no risk of metabolic syndrome, a low risk, a moderate risk and a high risk of metabolic syndrome.
EFFECT: method enables predicting the manifestation of metabolic syndrome by assessing the diagnostic criteria and conducting timely therapy for the purpose of preventing metabolic syndrome and cardiovascular complications.
3 tbl, 2 ex
SUBSTANCE: invention refers to medicine, namely to surgery, and can be used in cholecystectomy in patients with cholelithiasis. That is preceded by determining a patient's body weight index (BWI), glycaemia, glucosuria; blood pressure is measured; spinal osteochondrosis and gonarthrosis are detected. The derived results are assessed and scored. If the BWI equals to 28-30 kg/m2,10 points are assigned. If the BWI equals to 30-35 kg/m2,15 points are assigned. If the BWI is more than 35 kg/m2,20 points are assigned. If glycemia is more than 5.5 mmole/l, 3 points are assigned. If the patient suffers from glucosuria, 5 points are assigned. Arterial hypertension of more than 140/190 mm Hg requires assigning 3 points. The detected spinal osteochondrosis is assigned with 3 points, diagnosed gonarthrosis is assigned as 3 points. The derived points are summed up. If the total score is 23, a cholecystectomy with a biliopancreatic diversion is performed. If the total score is 14 to 22 points, laparoscopic cholecystectomy is performed. If the derived value makes 13 points, conventional cholecystectomy is performed.
EFFECT: invention provides selecting the type of cholecystectomy taking into account the metabolic status and a degree of obesity and as a consequence, normalizing the body weight and compensating the components of the metabolic syndrome.
2 tbl, 3 ex
SUBSTANCE: enzyme immunoassay is used to determine blood serum leptin in the boys at the age of 11-12 years old; the body weight index (BWI) is calculated; and leptin is related to the derived BWI. If the calculated numerical value is more than 0.4, enzyme immunoassay is used to measure dihydrotestosterone (DHT) and anti-Mullerian hormone (AMH); if the derived relation value makes 30.86 and less, delayed puberty is predicted in the prepubertal boys.
EFFECT: higher accuracy.
SUBSTANCE: invention represents a differential diagnostic technique for respiratory sarcoidosis and tuberculosis in males by biological fluid analysis; the technique involves measuring an amount of progesterone in the primary examination, and determining a probability coefficient P by formula
EFFECT: more reliable and objective diagnosis for the purpose of prescribing timely treatment and carrying out the adequate complex of therapeutic measures, simplifying the differential diagnosis of tuberculosis and respiratory sarcoidosis.
FIELD: medicine, psychiatry.
SUBSTANCE: one should isolate DNA out of lymphocytes of peripheral venous blood, then due to the method of polymerase chain reaction of DNA synthesis one should amplify the fragments of hSERT locus of serotonin carrier gene and at detecting genotype 12/10 one should predict the risk for the development of hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis.
EFFECT: more objective prediction of disease development.