Method for polymer embalmment of biomaterials

FIELD: medicine.

SUBSTANCE: invention refers to medicine. After fixing tissues and dissection, an anatomic preparation is immersed into an intermediate solution - acetone for 4 weeks, cooled to -20C, placed into a sealed metal chamber filled with a composition containing a medical siloxane polymer, a cross-linking agent and a catalyst. The composition is diluted in acetone in the concentration of 50-75%. An excessive pressure of 500 mm Hg is generated in the chamber; the preparation is cooled to a temperature of -8C. The composition fills the intercellular spaces of the preparation throughout 2-3 weeks; thereafter the preparation is cured in a thermostat at a temperature of 38C for 4 hours.

EFFECT: invention enables providing higher quality of the anatomic preparations.

1 dwg, 1 ex

 

The invention relates to medicine, and more specifically to the field of morphology, and is intended for the manufacture of polymer-containing anatomical micropreparative designed to teach students of medical, veterinary and biological Universities, as well as high school students of secondary educational institutions.

There is a method of soaking Biologicals silicone polymer in the manufacture of anatomical micropreparative, which sequentially displace the interstitial space filled with fixing tissue alcohol-formalin solution, initially at the intermediate solvent is acetone, then extract it from the tissues through the deep vacuum and its substitution on the polymer composition (G. von Hagens; K. Tiedemann, W. Kritz, The Current Potencial of by plastination. - Anatomy and Embriology (1987) 175:411-421).

The disadvantage of this method is a very slow process of impregnation of the biological tissue, because silicones do not dissolve with acetone, and replacing him on the polymer is only possible with long-term keeping of drugs in high vacuum (3-4 mm Hg). This requires expensive equipment and high energy costs. The result of a mechanical substitution of intermediate solvent for silicone composition becomes uneven impregnation p is eparate polymer and education repolimerizovanny areas of tissue in the depth of the drug.

Closest to the claimed method by way of a polymeric embalming is the way successive impregnation of the extracellular space in thin sections of organs and tissues of drugs solution celloidin increasing concentration (MA Subbotin, S. of Ligachev, YEAR Oganesyan, NM Alekseeva, A.F. Sukhanov. Histological technique. M., 1954, s-77).

The method does not require expensive equipment and high energy costs. However, the use as impregnating substance solution celloidin is not possible to completely remove the solvent from the tissues (as celloidin solid material), and this makes it difficult to demonstrate and storage of the drug in the air. In addition, the resulting products have a high hardness and brittleness.

The aim of the invention is to obtain anatomical preparations that retain their in vivo morphological properties intended for long-term use in the educational process in the study of human anatomy without additional measures aimed at preventing natural processes of decay and decomposition of tissue, formalin fixed solutions, highly toxic, both for students and teachers.

This goal is achieved by the fact that after making an anatomical preparation, his FIC is then formalisation solution substitute it in the tissues of the drug intermediate solvent - acetone impregnation anatomical micropreparative is carried out in a sealed, durable metal chamber in which an excessive pressure of 500 mm Hg, cooled to -8C composition containing a siloxane polymer medical devices, crosslinking agent and catalyst, diluted by 50 to 75% acetone, resulting in filling the intercellular space of the preparation of the polymer by the end of 2-3 weeks.

The method is implemented as follows. Anatomical microreport fix, dehydrate, degrease and give it the appropriate form known techniques. Immerse the product in a container with an intermediate solvent and place it in the freezer with a temperature of -20C for 4 weeks. Then put the product in a sealed, durable metal camera with a synthetic resin composition comprising a synthetic polymer medical devices, crosslinking agent and catalyst, diluted with the same solvent, which fills the intercellular space of the drug in the ratio of 50-75%. In metal sealed chamber lower the temperature to -8C, create and maintain it for 2-3 weeks gauge pressure of 500 mm Hg during this time is to extract the tion from the intercellular space of micropreparative solvent - acetone and replacing it on the siloxane polymer with a gradual increase in the concentration in the product.

Then remove the drug from the chamber, allowed to drain the excess polymer is removed by mechanical means its surplus with the drug, shape and position of the drug and are cured siloxane polymer in the anatomical micropreparative by heating thermostat up to 38C for 4 hours.

The claimed method is illustrated in the figure.

Microreport small intestine produced by the proposed method.

Here is a specific example illustrating the present invention. Pre-fixed in formalin mixture, uncovered a loop of the small intestine weight of 100 g, saturated intermediate solvent acetone for 4 weeks at -20C is immersed in a polymer composition comprising a synthetic rubber SKS-26, soloconsolidation acid and hydrogenoxalate oligomer, diluted with acetone in the ratio of composition as 75% to 25%, placed in a sealed, durable metal chamber, which creates excessive pressure of 500 mm Hg and a temperature of -8C, displacement of drug intermediate solvent. The result is filling the tissues of the drug and the intercellular space of the polymer by the end of 2-3 weeks.

After the end of the SRO is and impregnation of the drug is extracted from the thickened composition, mechanically remove the residues of the polymer from the surface of the mixture and place it in the incubator for 4 hours at a temperature of 38C and thus the vulcanization of the polymer to complete the preparation.

At the Department of operative surgery and clinical anatomy the course of innovative technologies Samara state medical University proposed method produced 7 preparations containing various kinds of rubbers medical and technical purposes. In all experiments, found that the impregnation of the intercellular space of the polymer and the medical and technical purposes proceeds uniformly throughout the thickness of the drug and compared to the known methods, in a shorter time. The preparation is distinguished by high strength and high wear resistance.

The method may find application in the work of the morphological departments of medical, veterinary and biological faculties of Universities, but also in the biological offices of colleges and secondary schools.

Way of a polymeric embalming anatomical preparations of synthetic polymer composition, wherein the drug after fixation and tissue preparation by cavity immersed in the intermediate solvent is acetone for 4 weeks, cooled to -20C, placed in garmet is know metal camera filled with a composition comprising a siloxane polymer medical devices, crosslinking agent and catalyst, diluted with acetone at a concentration of 50-75%, creates a gauge pressure of 500 mm Hg, cool the product to a temperature of -8C, with a composition fills the intercellular space of the drug for 2-3 weeks, after which the drug vulcanizer in a thermostat at a temperature of 38C for 4 hours.



 

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