Method for preserving anatomical preparations of bones

FIELD: medicine.

SUBSTANCE: method for preserving anatomical preparations of bones provides coating a defatted, dried bone cleaned of soft tissues with a preserving composition prepared of Dragon polymer adhesive and acetone taken in ratio 1:(1-3).

EFFECT: method provides mechanical stability and natural bone colour; the method is simple and safe in use.

3 ex


The present invention relates to medicine and can be used in anatomical museums, collections, departments of anatomy medical schools.

In medicine there are two widely used methods of cooking bone drugs by maceration (ognibene soft tissues in the water) and by digestion (Pakaluk B.C., frost GA, Kutya S.A. manual for making anatomical preparations, Simferopol, 2004). In further bone may be bleached in hydrogen peroxide solution or sunlight. Subsequent use of bone drugs in Museum collections and in practical classes with students of medical schools do not require additional methods of canning.

Despite its apparent strength, bone drugs, especially consisting of spongy bone tissue - the epiphyses of the long bones, pelvis, vertebrae, sacrum, sternum, clavicle subjected to intensive destruction as a result of studying their structure students in practical classes.

Given the great difficulty of obtaining a sufficient number of cadaveric material for educational and research purposes, extension of service life of bone material in the departments of normal anatomy of medical schools is a very important task.

Analogous to this is the manual is a traditional preparation of bone drugs by any of the methods described above without the use of further measures for their conservation (Kuznetsov LE, Khokhlov, V.V. Fadeev, S. p., Shigaev V.B. have been Embalming and restoration of corpses: a Guide. - M., 1999. - 496 S.).

The disadvantage of this method is the gradual destruction of bone tissue in places where bone beam thinning, on the bone surface has irregularities, numerous nutritional holes, small bony prominences. When working students with such bone drugs last experience ongoing traumatic bone microsattelite leading to the calving areas of bone.

The closest analog method is a method of plastination biological tissues (Gaivoronsky IV Grigoryan S. p. Way of polymeric embalming anatomical preparations siloxane compositions (patent RF №2426311), which involves several stages of fixation, dehydration, degreasing, soaking in the siloxane composition without the use of solvents and under the action of ultrasound and polymerization after giving the body the necessary provisions by heating the latter in a thermostat at 35-40°C. the Method of plastination usually used to obtain preparations of soft tissues, however, as described in the above patent is not limited to them. The disadvantage of this method are considerable complexity, the need for expensive reagents and equipment, significant the time for the preparation of drugs and substantial redundancy in the way, given that bone drug is quite solid and conservation needs in enhancing the strength properties of the surface and not in the elasticity of the latter.

Objectives of the invention are acceleration and reduction of the preservation of bone drugs in order to give them the superficial layers mechanical resistance and maintaining the natural color of bone.

The invention consists in the fact that must be cleaned of soft tissue, fat and dried bone (including can take a long time cooked bone preparation) impregnate one way or another (by means of a brush, spray, full immersion) solution, prepared by mixing 1 part of polymer glue "Dragon" and 1÷3 parts of acetone. Processing if necessary, conduct a factor of three. After each time the product is dried.

The technical result is achieved by using the cheap non-toxic widely available components and no need for complex equipment, for example, the vacuum chamber when a minor, in comparison with the closest analogue, the cost of time. Duration of preservation of the bone is from an hour up to 9-12 hours, much of which is occupied by a process of impregnation by dipping and drying of the drug that does not require direct human intervention.

The method implemented is tlaut as follows:

- pre-prepare a mixture of 1 part polymer glue "Dragon" and 1÷3 technical parts of acetone to produce a thorough homogenization of the mixture;

- cleaned from soft tissue fat and dried bone cover the resulting solution (by means of a brush, by spraying or full immersion);

within 7-60 minutes and is fully cured bone;

- if necessary in the presence of a significant amount of nutritional holes in bone, exostosis, irregularities, bone spicules, the processing is repeated after drying two or three times;

- creation of a full mechanical strength of the coating layer occurs within the first day after application.

The choice of a solution of polyvinyl acetate resin in an organic solvent due to its good by dissolving in acetone, as well as the ability to form a strong colorless layer, a well communicating with the surface of the bone tissue. The necessity of acetone in the mixture caused by the need to make the composition of the fluid, is able to penetrate deeply into the bone channels through nutritional holes, gently, without the formation of Saakov to cover the surface of the bone.

Below are examples illustrating the implementation of the proposed method.

Example 1. Took cadaveric femoral bone, scraped the soft tissues of maceration. Spent obese Rivonia in gasoline (the ratio of bone/petrol = 1/5 by weight) with a triple change of solvent within three days. Dried in the sun for 3 days. This bone was fully preserved anatomical structures of the medial and lateral namesake, big and small Vartely, well expressed gluteal tuberosity. In these structures there is a significant number of irregularities, microscopic bone protrusions, rich in holes. The surface of the bones in these rough places, clinging to the skin.

Prepared homogeneous solution containing 1 part of polymer glue "Dragon" and 1 part of technical acetone. Brush the surface of the bones covered by this solution. To said surface bearing a large number of bony prominence and nutritious holes, caused a greater amount of solution. After 7 minutes stated the complete drying of the surface of the bone. Additional processing was held twice. Inspection of the drug through the day showed the presence of a thin dense colorless, virtually undetectable by visual inspection of the surface. The degree of surface roughness of the drug significantly reduced. Within three years, bone is constantly used in the educational process, and violations of the integrity of bone tissue is not found.

Example 2. Took the sacrum, a long time in osteological collections of the Department of normal anatomy, GBOU VPO "Cubans the s state medical University" Ministry of health of Russia. All anatomically important structures of this bone was fully intact. On the whole surface of the bone there is a significant number of irregularities, microscopic bone protrusions, rich in holes. The surface of the bones had considerable roughness, clung to the skin. The sacrum is one of the most sensitive to mechanical influences bone preparation of a human skeleton.

Bone two hours completely immersed in an acetone solution for additional degreasing and penetration of the solvent in the smallest bone channels.

Prepared homogeneous solution containing 1 part of polymer glue "Dragon" and 3 parts of technical acetone. Bone was removed from the acetone, obtusely on the air for one hour and an additional two hours completely immersed in the prepared solution.

Next, the bone was removed from the solution, dried in air for 40 minutes and again covered from above with a solution containing 1 part of a solution of polyvinyl acetate resin in an organic solvent and 1 part of technical acetone to give a surface layer for additional strength.

Through daily inspection of the preparation showed the presence on the surface of a dense, colorless, low-observable by visual inspection, neudalyayuschiysya film. Stated the good, the smoothness of the drug to the touch. the year the drug was used in the learning process, fully preserved, remains strong and not lose the look.

Example 3. Took the bone preparation of the skull base, located in the osteological collection of the Department of normal anatomy of the state budgetary educational institution of higher professional education "the Kuban state medical University" Ministry of health of Russia. Some part of the bone had initial signs of destruction, especially evident in the structure of the small bones of the nasal cavity. The bone was well degreased and dried before the start of the process of conservation.

Immersion of the drug in preservative composition entirely recognized impractical due to the need of the cost of large amounts of reagents. Coating the surface with a brush turned out to be impossible because of the difficulty of access to many areas in the nasal cavity.

Prepared homogeneous solution containing 1 part of polymer glue "Dragon" and 3 parts of technical acetone. The drug is processed on all sides the resulting solution through a household spray to achieve the degree wet the surface. In twenty minutes the drug is completely dry. The same treatment was repeated twice more.

Through daily inspection of the preparation showed the presence on the surface of a dense, colorless, low-observable by visual inspection, neudalyayuschiysya film. Stated the increased smoothness of the drug.

After a year of use in the educational process of ven is at has no damage, the lacrimal bone and other bones of medial wall of orbit, collapsing in case of rough handling students with the drug in the first place, completely intact.

The proposed method can find application in the work of the departments of normal anatomy for prolongation of service life of bone drugs used in the educational process.

The method of conservation of bone anatomical preparations, including cleaning of the bone from the soft tissue, defatting, drying and drawing on the bone preparation of the composition of the preservative, characterized in that the composition of the preservative used polymer glue "Dragon", mixed with acetone in the ratio 1:(1-3), bone drug impregnated 1-3 times, after each time the product is dried.


Same patents:

FIELD: medicine.

SUBSTANCE: group of inventions refers to medicine, namely to cardiac surgery, and concerns creating cardiovascular homografts (CVHG) applied as vascular biological prostheses in cardiovascular surgeries. There are presented versions of a homograft representing a great vessel section or right and left ventricular outflow tracks taken from a dead body. The homograft is also characterised by min. 30-minute preparation in a medium containing gentamycin, diflucan and pH stabiliser. At a stage of preparation the homograft is processed with a medium of pH 7.0-7.4 representing a DMEM/F12 solution and containing gentamycin, diflucan, cephalosporin and metrogyl. Further, the homograft is kept in a medium containing the DMEM/F12 solution, gentamycin, diflucan and a phosphate buffer. There are also presented methods for producing the CVHG versions, as well as the media for the homograft treatment at the various stages of producing, keeping and handling.

EFFECT: creating the CVHG of more rigid structures resistant to damaging factors of the treatment, handling and storage media that improves their body functions.

16 cl, 10 ex, 9 tbl, 10 dwg

FIELD: medicine.

SUBSTANCE: before enzymatic treatment, a biotissue is placed into a hypertonic saline and exposed to ultrasound for 20-300 minutes. That is followed by terrilytin treatment in the concentration of 0.1-10 Production Units per 1g of the tissue, washing in an acetic acid solution and sodium hydrocarbonate, keeping in multiply changed glutaric dialdehyde solutions of the increasing concentrations, and sterilisation.

EFFECT: invention ensures maintaining a collagen-elastic structure of the biotissue, reducing a degree of its mineralisation, using fewer reactants for treatment, preparing a non-immunogenic biomaterial crossed throughout.

2 cl, 3 ex

FIELD: food industry.

SUBSTANCE: invention relates to antler deer farming, in particular, to conservation of a cooking pant water immediately on maral-breeding farm in the period of pant company. The method is as follows: combined with thermal treatment a cooking pant water used as preservative leaves of leather.

EFFECT: offered invention allows to in the period of pant company on maral-breeding farm keep for implementation the whole volume of a cooking pant water without modification of its organoleptic characteristics.

3 cl, 2 tbl, 1 ex

FIELD: medicine.

SUBSTANCE: course of radiation and/or chemotherapy is preceded by laparoscopic sampling of ovarian tissue from a cortical layer, and fragmentation by 2-5 mm2. One portion of the samples is taken to histological studies, while the other one is placed in cryotubes for two-staged preservation. A cryoprotector is presented by 10-14% dimethyl sulphoxide at the first stage, and 20% 2.5 M Saccharose and 29-32% dimethyl sulphoxide at the second stage. In the process of autografting, the ovarian tissue samples are de-frozen with using Kitazata medium and assessed by a histological examination. Immediately before the transplantation, a laparoscopic biopsy of the preserved ovaries is followed by the histological examination. If the examination result is satisfactory, a bed is formed in the cortical layer of each ovary to place therein 5 to 10 fragmented de-frozen samples. Bed edges are closed, processed with biologically neutral glue and clamped for the purpose of further visual inspection and follicle growth estimate of the transplant tissue with using a visual diagnostic technique. Hormone concentrations are monitored once a month until the hormone concentration actual for the moment of ovarian tissue sampling for preservation is reached.

EFFECT: method enables preserving or recovering the reproductive function in females suffering from oncological diseases by transplanting the de-cryopreserved ovarian tissue into the preserved ovaries that provides the adequate vascularisation and tissue survival rate.

4 cl, 3 tbl, 5 dwg, 3 ex

FIELD: packing industry.

SUBSTANCE: invention relates to package for containment and storage of liquids for freezing. Package (1) for containment and storage of a liquid (S) intended for freezing includes two sealing walls (4, 7) interconnected to form containment chamber (2) for liquid (S), one seat point (3) insulated from sealing chamber (2) for placement of thermal sensor device (T) measuring temperature of the liquid (S). Sealing walls (4, 7) include two outer walls (4) forming outer dimensions of the package itself, bearing walls (7) separating seat point (3) and capable of forming a through hole in the sealing chamber (2) comprised of two containment areas (2a). Outer walls (4) form edge (5) with inlet gap (6) for the liquid (S). Bearing walls feature a couple of first sides (7a) opposite to each other and a couple of second side (7b) opposite to each other.

EFFECT: enhanced reliability of temperature reading during supervision and certification.

8 cl, 2 dwg

FIELD: chemistry.

SUBSTANCE: invention relates to the chemistry of nitrogen-containing heterocyclic compounds, specifically derivatives of asymmetrical triazinones which can be used in agriculture. The growth stimulant contains a heterocyclic compound - 4-amino-1,4,5,6-tetrahydro-1,2,4-triazinone-5 - as an active component.

EFFECT: invention increases productivity of crops and has growth-stimulating activity.

4 tbl

FIELD: agriculture.

SUBSTANCE: device for freezing preserving of cell suspensions under pressure in an inert gas atmosphere - portable cryobarocontainer is made of stainless steel 12H18N10E and comprises front and rear panels. On the front panel there are eight holes for tie rods, and in the upper part there is a screw with a pusher for clamping the tube of the plastic container, through which the cell suspension is poured, and the inert gas is fed under pressure into a standard container "Kompoplast 300". The container is located in cryobarocontainer, and on the rear panel in the central part there is a niche, made exactly to dimensions and volume of the standard plastic container, and in the upper part there is a channel through which the tube passes from it, and in the upper and lower parts the niches there are elevations. From above of the rear panel there is a loop for hanging the entire device, and around the perimeter of the rear panel there are eight tension bolts fastened by the screw thread and welding in the rear panel. On the back of the rear panel the rear wall is attached with eight major tension bolts and mounting screws.

EFFECT: use of the present invention enables to carry out freezing according to various programs.

2 dwg

FIELD: chemistry.

SUBSTANCE: dosage unit in form of pressed tablet for delayed release of insecticide contained evaporating insecticidal preparation and solid inert base. Solid base contains two-component system, which includes material for giving volume and material, providing porosity. Material for giving volume is selected from group of filling agents of direct pressing. Porosity-providing material is represented by carbonate or bicarbonate. Device of insecticide supply contains capacity for insecticide. To obtain dosage unit ingredients are mixed and tablets are formed at temperature which is higher than temperature of insecticide melting. Insecticide measuring apparatus contains dosage unit as single source of insecticide. Composition is in form of tablet, placed near heater. Device contains fork, providing electric connection with heater, and cap for tablet, containing casing and cover.

EFFECT: elaboration of tablet for delayed release of insecticide.

13 cl, 2 dwg, 1 ex

FIELD: medicine.

SUBSTANCE: invention relates to medicine, veterinary and biology. Sample fixation, decalcification, washing with water, dehydration in alcohol solutions and pouring with paraffin are performed. Sample fixation is performed for 24 hours in alcohol-based molecular fixing agent FineFix, which contains FineFix and 96° alcohol in ratio 1:2.5. Decalcification is carried out for 2-5 days in 5-8% buffered solution of formic acid with daily replacement of decalcifying solution and control of decalcification completeness. Ratio sample:decalcifying solution constitutes 1:20. When decalcification is finished, sample washing with water is performed and sample is re-submerged into alcohol solution of molecular fixing agent FineFix for 6-12 hours before dehydration stage. Set for preparation of bone tissue preparation contains alcohol-based molecular fixing agent FineFix, concentrated solution of decalcifying agent, prepared with ratio 40 g of sodium citrate, 100 ml of 90% solution of formic acid, 300 ml of distilled water, and working solutions for control of decalcification completeness, containing saturated ammonium oxalate solution and 25% water solution of ammonia.

EFFECT: invention makes it possible to obtain high-quality preparations suitable for further histological and immunohistological analysis without application of highly toxic components.

2 cl, 2 dwg

FIELD: chemistry.

SUBSTANCE: invention relates to ophthalmology. The cornea storage solution contains a mixture of biological media Ml99 and DMEM, chondroitin sulphate, L-alanyl-L-glutamine, 4-(2-hydroxyethyl)-1-piperazine ethane sulphonic acid (HEPES), sodium pyruvate, sodium β-hydroxybutyrate, gentamicin sulphate, amphotericin B, 2,3,5,7,8-pentahydroxy-6-ethyl naphthalenedione-1,4 (echinochrome A) and hydroxyethyl starch, with the following ratio of components, wt %: medium M199 0.64; medium DMEM 0.447; hydroxyethyl starch 5-10; chondroitin sulphate 2.5; echinochrome A 0.001-0.01; L-alanyl-L-glutamine 0.05425; buffer HEPES 0.5958; sodium pyruvate 0.0055; sodium β-hydroxybutyrate 0.151; gentamicin sulphate 0.01; amphotericin B 0.0000250; distilled water - up to 100.

EFFECT: invention increases storage life of cornea.

1 dwg, 1 tbl, 1 ex

FIELD: medicine.

SUBSTANCE: method involves using product containing spermatozoa treated by drying with freezing to humidity level of 1% and having injured membrane or spermatozoon head so that spermatozoon nucleus retains its genetic validity enough for fertilization. The spermatozoon heads fertilize an isolated oocyte after rehydration and microinjection being done. The retained genetic integrity is enough for fertilizing an oocyte and producing living descendants. Method involves collecting living mature spermatozoa, making spermatozoa suspension in special purpose physiological medium, freezing the spermatozoa suspension for producing frozen spermatozoa, drying the frozen spermatozoa or spermatozoa heads in vacuum to humidity level of 1%, making rehydration of spermatozoa or spermatozoa heads with injured membrane and selecting those retaining nucleus of genetic integrity. The selected spermatozoa or spermatozoa heads are used for fertilizing isolated oocytes with living descendants being produced.

EFFECT: enhanced effectiveness in producing living descendants.

39 cl, 5 dwg, 1 tbl

FIELD: medicine, biology.

SUBSTANCE: invention relates to nutrient medium used for accumulation of cells for the following cytological and/or immunocytochemical analysis carrying out. Invention relates to medium containing salts NaCl, KCl, anhydrous CaCl2, MgSO4 x 6 H2O, MgCl2 x 6 H2O, Na2HPO4 x 2 H2O, KHPO4, NaHCO3, and also glucose and Henx's solution, 10% albumin solution and polyglucin taken in the ratio 1:1:1. Invention provides enhancing the preservation of cells.

EFFECT: improved an valuable properties of nutrient medium.

3 ex

FIELD: medicine.

SUBSTANCE: method involves exposing blood to be preserved, to uniform magnetic field of 17000±100 nTesla units intensity and 2.5-3 Hz pulsation frequency during at least 2 h and then cooling it.

EFFECT: prolonged storage period; high erythrocyte resistance and osmoresistance.

FIELD: anatomic embalming facilities.

SUBSTANCE: solution consists acetone as principal component producing dehydration effect on tissues and, in order to enhance defatting property of solution, the latter further contains hexane possessing strong defatting capacity, all components of solution being taken in specified proportions.

EFFECT: increased wear resistance of polymeric anatomic preparations and prolonged storage time thereof.

FIELD: anatomic embalming facilities.

SUBSTANCE: solution consists acetone as principal component producing dehydration effect on tissues and, in order to enhance defatting property of solution, the latter further contains hexane possessing strong defatting capacity, all components of solution being taken in specified proportions.

EFFECT: increased wear resistance of polymeric anatomic preparations and prolonged storage time thereof.

FIELD: agriculture.

SUBSTANCE: air-dried raw material from terraneous parts of Echinacea purpurea plants is extracted with 90-95 % ethanol in ethanol/raw material ratio of 59-61:1, respectively. Extract is acidified with acid to obtain pH not more than 5.0. Extract is purified with polymeric sorbent and concentrated by vaporization. Obtained plant growth controlling agent contains chicory, chlorogenic and caffeic acids. Growth-controlling preparation contains growth-controlling complex in amount of 0.009-0.11 mg/ml and oxyethylated alkylphenol surfactant in ratio of 1:1. Claimed complex is useful in plant and scrub treatment and in seed soak.

EFFECT: preparation useful as immunomodulator and antistress agent.

6 cl, 6 ex

FIELD: cryobiology, cryomedicine.

SUBSTANCE: method involves addition cryoprotective medium to sperm and its freezing with liquid nitrogen vapors at super high rates of freezing. Also, invention relates to composition of cryoprotective medium comprising glycerol ester, tris-buffer, EDTA, lecithin, kanamycin sulfate and water in special containers. Method provides significant enhancing viability and motility of spermatozoons and to increase frequency of pregnancy occurring after impregnation with sperm cryopreserved by proposed method.

EFFECT: improved cryopreserving method.

2 cl, 3 tbl, 3 ex

FIELD: processes for treating organic material before decomposition or sublimation drying procedures.

SUBSTANCE: method involves exposing organic material, preferably in cooled or frozen state, to splitting treatment, followed by sublimation drying process; placing organic material for decomposition. Apparatus for decomposition has closed reservoir equipped with equipment for connection with sources of vacuum, liquid nitrogen, high-pressure water jets or high-pressure steam, or laser with high radiation energy, or vegetable oil under high pressure. Apparatus is further provided with supersonic probe. Apparatus is tunnel-type reservoir equipped with vibration device.

EFFECT: increased efficiency in decomposing of organic material which may be assimilated by plants.

12 cl, 1 dwg, 1 tbl

FIELD: agriculture, in particular, reprocessing, preservation and storage of natural raw material, in particular, Siberian deer velvet antlers.

SUBSTANCE: method involves cyclic thermal processing of antlers in vacuum drier followed by cooling, with drying process being provided in two steps including first step carried out at temperature of 65°C in chamber and vacuum value of 0.094-0.096 MPa for 4 hours, followed by cooling upon expiration of indicated time at temperature of 15-20°C for 12-24 hours, with cycle of operations being repeated until removal of 35-40% of moisture, and second step, wherein temperature in chamber is reduced to 45°C and drying process is continued at the same vacuum parameters for 7 hours, with following carrying out of cooling process under conditions similar to those of the first step, and processing of raw material with the same parameters until normal moisture content is 12-19%. Method allows drying process to be reduced to 6-9 days depending on antler size and weight parameters.

EFFECT: reduced loss of biologically active substances during reprocessing and preservation of antlers, reduced time for drying of basic material and reduced labor intensity.

2 cl, 3 tbl, 2 ex

FIELD: medicine, physiology.

SUBSTANCE: invention relates to the development of agent as a protective solution used for preserving leukocytes at negative temperatures and their retaining in physiologically active state after escape from cold hypobiosis. The solution (pH 7.0-7.4) contains glycerol, hydroxymethylpyrimidine succinate and modegel taken in the optimal ratio. The protective solution provides safety of high percent of functionally active leukocyte cells.

EFFECT: valuable properties of solution.

3 tbl, 2 ex