Method for preserving anatomical preparations of bones

FIELD: medicine.

SUBSTANCE: method for preserving anatomical preparations of bones provides coating a defatted, dried bone cleaned of soft tissues with a preserving composition prepared of Dragon polymer adhesive and acetone taken in ratio 1:(1-3).

EFFECT: method provides mechanical stability and natural bone colour; the method is simple and safe in use.

3 ex

 

The present invention relates to medicine and can be used in anatomical museums, collections, departments of anatomy medical schools.

In medicine there are two widely used methods of cooking bone drugs by maceration (ognibene soft tissues in the water) and by digestion (Pakaluk B.C., frost GA, Kutya S.A. manual for making anatomical preparations, Simferopol, 2004). In further bone may be bleached in hydrogen peroxide solution or sunlight. Subsequent use of bone drugs in Museum collections and in practical classes with students of medical schools do not require additional methods of canning.

Despite its apparent strength, bone drugs, especially consisting of spongy bone tissue - the epiphyses of the long bones, pelvis, vertebrae, sacrum, sternum, clavicle subjected to intensive destruction as a result of studying their structure students in practical classes.

Given the great difficulty of obtaining a sufficient number of cadaveric material for educational and research purposes, extension of service life of bone material in the departments of normal anatomy of medical schools is a very important task.

Analogous to this is the manual is a traditional preparation of bone drugs by any of the methods described above without the use of further measures for their conservation (Kuznetsov LE, Khokhlov, V.V. Fadeev, S. p., Shigaev V.B. have been Embalming and restoration of corpses: a Guide. - M., 1999. - 496 S.).

The disadvantage of this method is the gradual destruction of bone tissue in places where bone beam thinning, on the bone surface has irregularities, numerous nutritional holes, small bony prominences. When working students with such bone drugs last experience ongoing traumatic bone microsattelite leading to the calving areas of bone.

The closest analog method is a method of plastination biological tissues (Gaivoronsky IV Grigoryan S. p. Way of polymeric embalming anatomical preparations siloxane compositions (patent RF №2426311), which involves several stages of fixation, dehydration, degreasing, soaking in the siloxane composition without the use of solvents and under the action of ultrasound and polymerization after giving the body the necessary provisions by heating the latter in a thermostat at 35-40°C. the Method of plastination usually used to obtain preparations of soft tissues, however, as described in the above patent is not limited to them. The disadvantage of this method are considerable complexity, the need for expensive reagents and equipment, significant the time for the preparation of drugs and substantial redundancy in the way, given that bone drug is quite solid and conservation needs in enhancing the strength properties of the surface and not in the elasticity of the latter.

Objectives of the invention are acceleration and reduction of the preservation of bone drugs in order to give them the superficial layers mechanical resistance and maintaining the natural color of bone.

The invention consists in the fact that must be cleaned of soft tissue, fat and dried bone (including can take a long time cooked bone preparation) impregnate one way or another (by means of a brush, spray, full immersion) solution, prepared by mixing 1 part of polymer glue "Dragon" and 1÷3 parts of acetone. Processing if necessary, conduct a factor of three. After each time the product is dried.

The technical result is achieved by using the cheap non-toxic widely available components and no need for complex equipment, for example, the vacuum chamber when a minor, in comparison with the closest analogue, the cost of time. Duration of preservation of the bone is from an hour up to 9-12 hours, much of which is occupied by a process of impregnation by dipping and drying of the drug that does not require direct human intervention.

The method implemented is tlaut as follows:

- pre-prepare a mixture of 1 part polymer glue "Dragon" and 1÷3 technical parts of acetone to produce a thorough homogenization of the mixture;

- cleaned from soft tissue fat and dried bone cover the resulting solution (by means of a brush, by spraying or full immersion);

within 7-60 minutes and is fully cured bone;

- if necessary in the presence of a significant amount of nutritional holes in bone, exostosis, irregularities, bone spicules, the processing is repeated after drying two or three times;

- creation of a full mechanical strength of the coating layer occurs within the first day after application.

The choice of a solution of polyvinyl acetate resin in an organic solvent due to its good by dissolving in acetone, as well as the ability to form a strong colorless layer, a well communicating with the surface of the bone tissue. The necessity of acetone in the mixture caused by the need to make the composition of the fluid, is able to penetrate deeply into the bone channels through nutritional holes, gently, without the formation of Saakov to cover the surface of the bone.

Below are examples illustrating the implementation of the proposed method.

Example 1. Took cadaveric femoral bone, scraped the soft tissues of maceration. Spent obese Rivonia in gasoline (the ratio of bone/petrol = 1/5 by weight) with a triple change of solvent within three days. Dried in the sun for 3 days. This bone was fully preserved anatomical structures of the medial and lateral namesake, big and small Vartely, well expressed gluteal tuberosity. In these structures there is a significant number of irregularities, microscopic bone protrusions, rich in holes. The surface of the bones in these rough places, clinging to the skin.

Prepared homogeneous solution containing 1 part of polymer glue "Dragon" and 1 part of technical acetone. Brush the surface of the bones covered by this solution. To said surface bearing a large number of bony prominence and nutritious holes, caused a greater amount of solution. After 7 minutes stated the complete drying of the surface of the bone. Additional processing was held twice. Inspection of the drug through the day showed the presence of a thin dense colorless, virtually undetectable by visual inspection of the surface. The degree of surface roughness of the drug significantly reduced. Within three years, bone is constantly used in the educational process, and violations of the integrity of bone tissue is not found.

Example 2. Took the sacrum, a long time in osteological collections of the Department of normal anatomy, GBOU VPO "Cubans the s state medical University" Ministry of health of Russia. All anatomically important structures of this bone was fully intact. On the whole surface of the bone there is a significant number of irregularities, microscopic bone protrusions, rich in holes. The surface of the bones had considerable roughness, clung to the skin. The sacrum is one of the most sensitive to mechanical influences bone preparation of a human skeleton.

Bone two hours completely immersed in an acetone solution for additional degreasing and penetration of the solvent in the smallest bone channels.

Prepared homogeneous solution containing 1 part of polymer glue "Dragon" and 3 parts of technical acetone. Bone was removed from the acetone, obtusely on the air for one hour and an additional two hours completely immersed in the prepared solution.

Next, the bone was removed from the solution, dried in air for 40 minutes and again covered from above with a solution containing 1 part of a solution of polyvinyl acetate resin in an organic solvent and 1 part of technical acetone to give a surface layer for additional strength.

Through daily inspection of the preparation showed the presence on the surface of a dense, colorless, low-observable by visual inspection, neudalyayuschiysya film. Stated the good, the smoothness of the drug to the touch. the year the drug was used in the learning process, fully preserved, remains strong and not lose the look.

Example 3. Took the bone preparation of the skull base, located in the osteological collection of the Department of normal anatomy of the state budgetary educational institution of higher professional education "the Kuban state medical University" Ministry of health of Russia. Some part of the bone had initial signs of destruction, especially evident in the structure of the small bones of the nasal cavity. The bone was well degreased and dried before the start of the process of conservation.

Immersion of the drug in preservative composition entirely recognized impractical due to the need of the cost of large amounts of reagents. Coating the surface with a brush turned out to be impossible because of the difficulty of access to many areas in the nasal cavity.

Prepared homogeneous solution containing 1 part of polymer glue "Dragon" and 3 parts of technical acetone. The drug is processed on all sides the resulting solution through a household spray to achieve the degree wet the surface. In twenty minutes the drug is completely dry. The same treatment was repeated twice more.

Through daily inspection of the preparation showed the presence on the surface of a dense, colorless, low-observable by visual inspection, neudalyayuschiysya film. Stated the increased smoothness of the drug.

After a year of use in the educational process of ven is at has no damage, the lacrimal bone and other bones of medial wall of orbit, collapsing in case of rough handling students with the drug in the first place, completely intact.

The proposed method can find application in the work of the departments of normal anatomy for prolongation of service life of bone drugs used in the educational process.

The method of conservation of bone anatomical preparations, including cleaning of the bone from the soft tissue, defatting, drying and drawing on the bone preparation of the composition of the preservative, characterized in that the composition of the preservative used polymer glue "Dragon", mixed with acetone in the ratio 1:(1-3), bone drug impregnated 1-3 times, after each time the product is dried.



 

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