Method of manufacturing replica for analysis of microstructure of frozen rocks in scanning electron microscope

FIELD: chemistry.

SUBSTANCE: sample surface is obtained by split of a monolith, after which a successive application of polymer solutions on the sample is performed. As the polymer solutions used are: first, a 0.5% solution of formvar in dichloroethane, and then, a 2% solution of polymethylacrylate in dichloroethane with an exposure of the sample at a negative temperature to dry each layer and obtain a solid film. After drying the obtained film with particles of soil is detached from the sample and analysed in a scanning electron microscope.

EFFECT: method improvement.

4 dwg

 

The invention relates to methods for producing replicas of polymeric materials for the study of the microfabrics of frozen rocks in the scanning electron microscope.

A known method of manufacturing thin slices frozen at subzero temperatures of rocks to study the microfabrics with light (polarizing) microscope [Rogov, V.V. Study of the microfabrics of frozen rocks (methods results Outlook) // materials of III International conference on permafrost, Science, Coll. the CTD. Novosibirsk, 1978, p.95-98].

However, the known method has a limited scope: the fragility of the sample, heating the surface with subsequent melting ice affects the image and distorts the original structure of the soil; the inability of most studies of small-size components - clay particles and microorganisms.

There is also known a method of study of the microfabrics of frozen rocks in thin sections using light (polarizing) microscope, which after removal of the ice (wydawania or freeze-drying) of the pore space of the sample is filled with resin for fixing. [Rogov VV Applying etching to study the structure of ice in frozen sediments // Vestnik Mosk. University, ser. geogr., 1972, No. 3, p.101-102].

However, this known method has limited areas of the applications: the distortion of the original structure of the soil after the removal of ice, the inability to study the crystalline structure of ice, the spatial layout of the components of the soil relative to the crystals, as well as most small components - clay particles and microorganisms.

The technical result of the claimed invention consists in the preservation of labile components of the frozen ground, extend the scope of the method.

This technical result is achieved by the inventive method of manufacturing replicas for the study of the microfabrics of frozen soils in the scanning electron microscope the sample surface by cleavage of the monolith, then perform sequential drawing on a sample of polymer solutions, which use the first 0.5% solution formvar in dichloromethane and then 2% solution of polymethacrylate in dichloroethane with keeping the sample at subzero temperatures for the drying of each layer and obtain a solid film, after drying, prepare the chin from a sample of the finished film with soil particles and examined under scanning electron microscope.

The production of replicas at low temperatures to preserve the undisturbed structure of the frozen ground leads to the evaporation of ice through the first layer (film formware) and the formation on its surface of a hole of a certain geometric shape according to the size and shapes of the ice crystals, that allows you to get information about its crystal structure. The second layer (film polymethylacrylate) allows to fix the particles of frozen soil in a natural undisturbed state. After drying both layers at subzero temperatures the finished film prepare the chin and examined under scanning electron microscope. The film repeats the pattern of the microfabrics of frozen rocks, therefore, bears the name of the replica (from lat. replico - repeat, eng. replica - print) and can be studied in a scanning electron microscope at various magnifications.

The invention is illustrated illustrative materials, in which figure 1 presents the photo of the replica of the frozen sample with preservation of the natural relief of the surface of the chip; figure 2 is a micrograph of a replica of the frozen ground (figure 1) in the scanning electron microscope; figure 3 is a micrograph of the holes etched in the ice crystals; figure 4 is a micrograph of Bacillus in a bed of ice in frozen soils.

An example of manufacturing of the replicas for the study of the microfabrics of frozen rocks in the scanning electron microscope.

Monolith frozen ground split at subzero temperatures. On fresh fracture of the obtained sample is applied polymer solution (for example, 0.5% solution formvar in dichloroethane) by spread on the surface and leave to complete you is ahania film. Then similarly put the second layer of polymeric material (for example, a 2% solution of polymethacrylate in dichloroethane) and again leave to dry film. The sample is maintained at negative temperatures for the drying of each layer and obtain a solid film. The finished replica adhesively fixed thereto soil particles prepare the chin from a sample cut to a size of the object stage and examined under scanning electron microscope. Thus, the finished replica is a polymer film with soil particles and holes etched on its surface according to the size and shape of ice crystals. We used vacuum scanning electron microscope TM without cryogenic cooling replicas.

Thus, the use of the invention greatly enhances the ability to study the unstable components of the microfabrics of frozen soils in the scanning electron microscope, save made replica for further research.

A method of manufacturing replicas for the study of the microfabrics of frozen soils in the scanning electron microscope, characterized in that the surface of the sample by cleavage of the monolith, then perform sequential drawing on a sample of polymer solutions, which use the form first 0.5% solution formvar in dichloroethane, and then 2% solution of polymethacrylate in dichloroethane with keeping the sample at subzero temperatures for the drying of each layer and obtain a solid film, after drying, prepare the chin from a sample of the finished film with soil particles and examined under scanning electron microscope.



 

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