Treatment of infection of human or animal body surface

FIELD: medicine.

SUBSTANCE: invention relates to medicine. Described is method of treating infection of human or animal body surface, in particular, contamination with fungi, including application of water liquid on infected body surface, for instance, nail area, with further application of bandage, which includes hydrogen peroxide source. Combinations of bandage liquid for application in method are also provided.

EFFECT: combination makes it possible to considerably reduce or eliminate infection in nail area.

16 cl, 1 tbl, 2 dwg, 2 ex

 

The technical field

This invention relates to the treatment of infections of the body surface of a human or animal, in particular infection by fungi nail area.

Background of the invention

Healthy nails in apparently good condition are important and highly valued aspects of the appearance of the person. Often the appearance, strength and health of nails may be adversely affected by infecting cells of pathogenic fungi, usually of the genus Trychophyton, and there is a great need for treatments that improve the appearance of affected nails by removing infecting fungi. Despite the market presence of numerous therapeutic agents, there is widespread dissatisfaction with existing technologies and products.

Systemically delivered tools can attain the nail region through the bloodstream, but poor penetration into the nail area of flow and serious side effects limit the usefulness of the approach.

Infected mushroom nails often become porous or open due to the impact of penetrating fungus. Thus, often the nail is jointly colonized by bacteria, which can exacerbate the damaging effects of mold, providing additional destructive enzymes and locally active toxins.

Well Izv the STN, even if the fungi nail reduced by known methods of treatment, it is rarely completely eliminated, and for infections tend to recur soon after stopping treatment.

Brief description of the invention

The inventors have found that the active ingredients are not easily penetrate into the nail, and a small amount, if any such there are, of material attached to the upper surface, reaches beneath structures where fungal cells can be in relative safety.

In the first aspect of the invention relates to a method for the treatment of infections of the body surface of a human or animal, such as infection by fungi, including the application of liquid water on the surface of infected body, for example the nail area, with the subsequent application of a dressing, comprising a source of hydrogen peroxide.

In the second aspect of the invention relates to the combination of water and liquid bandage, comprising a source of hydrogen peroxide in the treatment of infections of the body surface of a human or animal, for example, infected mushrooms, in particular, but not exclusively, the nail area of a human or animal.

In the third aspect of the invention relates to the use of a combination of water and liquid bandage, comprising a source of hydrogen peroxide, in the manufacture of a drug product DL the treatment of infections of the body surface of a human or animal, for example, infection by fungi, in particular but not exclusively, the nail area of a human or animal.

Through the application of aqueous liquid on the surface of infected body surface of the body softens and becomes more porous. For example, this may allow the liquid to penetrate deep into the nail area through the existing porosity. Thus, the fluid provides water flow to the interior surface of the body, such as the nail area.

Subsequent application of a dressing containing a source of hydrogen peroxide, thus leading to effective diffusion of hydrogen peroxide educated on water flow in the inner less accessible parts of the body surface, for example the nail area, which allows the hydrogen peroxide to penetrate deeper, allowing you to significantly reduce or eliminate the infection, such as fungi in all the composite parts, such as the nail area.

The nails have a characteristic anatomy and composition, which should be taken into account when considering new approaches to the treatment directed on the nail plate and located beneath the nail bed, as well as all associated structures. The most visible part of the nail, the nail plate consists of hard keratinized structure, composed of dead keratinized cells (corneocytes), popped up the C matrix at the base of the nail. A large part of the nail plate is semi-transparent, allowing the color of the blood supply in the dermis Shine through, giving a pinkish color. The nail itself is relatively devoid of moisture, but upon contact with water, it can become relatively gidratirovannym, taking relaxed and flexible state.

Nail wall, the fold of skin that covers the side of the nail, hold the nail plate in place and protects its edges. Only live reproducibles part of the nail is the nail matrix, which is located directly under the cuticle. New cells are formed in this place and constantly moving as they grow, with the formation of the nail plate. Matrix addition is supplied with nerves, and is also replete with blood vessels to provide cells with food and oxygen.

The nail plate rests on the nail bed, which is continuous with the matrix. It is also abundantly supplied with blood vessels and nerves. Its surface formed with multiple parallel folds, exact match with pleats on the bottom surface of the nail plate. The cuticle is part of the epidermis of the skin that covers the nail. It protects the matrix from bacteria attacks and physical damage.

Cells of the fungus can grow in any part of these structures, and to any treatment it is very important to get all the Uch is tough in order to remove any residual foci of infection, and remove the infection in major parts of the nail plate.

The expression "the nail region" is defined herein to include nail plate, nail bed, nail matrix, nail fold and cuticle.

The invention also relates to a method for processing cells infected with human papillomavirus, including the application of the aqueous liquid as defined in this document, infected cells with subsequent dressing, comprising a source of hydrogen peroxide, as defined in this document.

The invention also relates to the combination of the aqueous liquid as defined in this document, and bandages comprising a source of hydrogen peroxide, as defined herein, for the treatment of cells infected with human papilloma virus.

In a preferred embodiment, the aqueous liquid comprises the enzyme peroxidase. Peroxidase penetrates the surface of the body, such as the nail area.

The enzyme peroxidase after penetration into the inner body surface area, such as a nail, remains essentially inactive as long as the peroxide no diffuses through the water way of dressing. In the presence of peroxidase oxidative effects of hydrogen peroxide increase.

Peroxidase, thus, as was installation is Leno significantly enhances the activity, in particular fungicidal activity, hydrogen peroxide, because it catalyzes the oxidation of susceptible, but the necessary molecules of the fungus on or in the cell membrane and/or cytoplasm of the cells of the fungus.

Any suitable peroxidase can be used, including lactoperoxidase, horseradish peroxidase, iodide peroxidase, hydrogen chloride peroxidase and myeloperoxidase. However, lactoperoxidase and horseradish peroxidase currently preferred.

The concentration of the enzyme peroxidase in aqueous liquid is preferably in the range from 1 to 1000 μg/ml, preferably from 50 to 1000 μg/ml, more preferably from 100 to 500 µg/ml

Water liquid also preferably contains surfactants and/or solvents that were found, increase the penetration of liquid into the surface of the body, such as a nail.

The bandage is preferably in the hydrated state to ensure that the hydrogen peroxide can effectively diffuse into the nail area immediately after dressing. Sufficient water is necessary in the bandage for formation of a contact liquid connection between the nail area and bandage.

Preferably, the osmotic strength of the aqueous liquid is the same or similar to that in the liquid bandage on the I gain the desired flow of fluid and dissolved substances.

The bandage is preferably water gives the nail region using, which is achieved by selection of appropriate osmotic properties in a known manner.

The bandage material can be in the form of a hydrogel, sponge, foam, or any other form hydrophilic matrix, which may contain enough water to allow a controlled flow of diffusion between the bandage and the nail area. Preferably, the bandage contains dissolved substances that serve to regulate the passage of hydrogen peroxide, for example, through formation of hydrogen bonds that can be achieved by appropriate concentrations of polymers, such as polysaccharides, including glycosaminoglycans.

The bandage may include a wet cotton bandage or may contain structural pad material with the wet ingredients. Preferably, however, to bandage contained one or more gels are water-based or water, also referred to as hydrated hydrogels. Such gels can be formed from various materials and may contain different reagents, as will be discussed below.

Typically, the dressing will be in the form of a plate, layer or film. Bandage, alternatively, it may be in the form of amorphous gel or lotion, preferably a hydrogel that does not have some form of ricercari, which can be deformed and which can be shaped in three dimensions, including by extrusion through a nozzle. Amorphous gels usually are not sewn or have low levels of cross-linking. Razzhizhayuschiesya shear amorphous gel can be applied. This gel is a liquid when subjected to shear stress (for example, when poured or extruded through the nozzle), but he freezes when static.

Suitable hydrated hydrogels are disclosed in WO No. 03/090800. The hydrated hydrogel easily include hydrophilic polymeric material. Suitable hydrophilic polymers include polyacrylates and methacrylates, for example, supplied First Water Ltd in the form of a hydrogel plates, including poly-2-acrylamide-2-methylpropanesulfonic acid (polyAMPS) or a salt thereof (e.g. as described in WO No. 01/96422), polysaccharides, such as polysaccharide gums, in particular xanthan gum (e.g., available under the trade name Keltrol), various sugars, polycarboxylic acids (e.g., available under the trademark Gantrez-169 BF from ISP Europe), the copolymer metilfenidato ether and maleic anhydride (e.g., available under trademark Gantrez 139 having a molecular weight in the range from 20000 to 40000), polyvinylpyrrolidone (e.g., in the form of commercially available brands, known as PP K-30 and PVP K-90), the polyethylene oxide (e.g., available under the trademark Polyox WSR-301), polyvinyl alcohol (e.g., available under the trademark Elvanol), crosslinked polyacrylic polymer (e.g., available under the trademark Carbopol EZ-1), cellulose and modified cellulose, including hydroxypropylcellulose (e.g., available under the trademark Klucel EEF), carboxymethylcellulose sodium (e.g., available under the trademark Cellulose Gum 7LF) and hydroxyethyl cellulose (e.g. available under the trade name NATROSOL 250 LR).

A mixture of hydrophilic polymeric materials can be applied in a gel.

In hydrated hydrogel of a hydrophilic polymeric material is a hydrophilic polymeric material preferably is present in a concentration of at least 0.1 percent, preferably at least about 0.5%, preferably at least 1%, preferably at least 2%, more preferably at least 5%, even more preferably at least 10% or at least 20%, preferably not less than 25% and even more preferably at least 30% by weight based on the total weight the gel. Even larger amount to about 40% by weight based on the total weight of the gel can be used.

The preferred hydrated hydrogel comprises poly-2-acrylamide-2-methylpropanesulfonic acid (S) or a salt thereof, preferably to the number of about 20% by weight of the total weight of the gel.

The source of hydrogen peroxide may include hydrogen peroxide per se or hydrogen peroxide in combination with or in conjunction with another element. Alternatively, a source of hydrogen peroxide can be a means of generating hydrogen peroxide.

In a preferred embodiment, the source of hydrogen peroxide are the means of generating hydrogen peroxide, including oxidoreductase enzyme, a source of oxygen and a source of substrate for the enzyme. Oxidoreductase enzyme catalyzes reaction of the corresponding substrate with oxygen to produce hydrogen peroxide.

Oxidoreductase enzymes suitable for use in the invention, and the corresponding substrates (which are present in the blood and tissue fluids) contain the following:

EnzymeSubstrate
Glucose oxidaseβ-D-glucose
HexosaminidaseHexose
CholesterolaemiaCholesterol
GalactosidaseD-galactose
PianosoundsPyranose/td>
CholinesteraseCholine
PiruwatkinazaPyruvate
GlycosidaseGlycolate
Oxidase amino acidsAmino acid

In the currently preferred oxidoreductase enzyme is glucose oxidase. It catalyzes the reaction of the substrate β-D glucose with the formation of hydrogen peroxide and gluconic acid.

Can be applied mixture oxidoreductase enzymes.

Oxidoreductase enzyme and glucose can be thoroughly mixed, optional, along with a source of oxygen. The oxygen may be provided in any suitable oxygen donor, but convenient source is atmospheric oxygen.

If the source of oxygen is oxygen in the atmosphere, dressing preferably includes separate first and second layers. The first layer includes oxidoreductases enzyme and is located near the outer parts of the bandage, that is removed when applying the nail area, where the levels of atmospheric oxygen are the most high. The second layer includes the source substrate and located close to the inner parts of the bandage that is adjacent to the nail area, the AK produced by hydrogel peroxide can act directly in the nail area.

In the preferred form of the layered case for both the first and second layers include crosslinked hydrated hydrogels. Hydrogels can be filled by mechanically reinforced structure, such as a layer of cotton gauze or inert flexible grid, for example, to provide a structurally reinforced hydrogel layer or plate.

Alternatively, the first layer containing the enzyme may be in a dried state, but be placed in fluid communication with the second layer during application, causing the water moves to the first layer with the hydration of the enzyme.

In layered embodiment, it is preferable that the first layer was relatively thin, i.e. from 0.01 to 2.0 mm, and the second layer was relatively thick, i.e. from 0.5 to 5.0 mm, If the first layer is gidratirovannym a hydrogel, then its thickness is preferably from 0.1 to 2.0 mm, If the first layer is a dry film, then its thickness is preferably from 0.01 to 0.1 mm

The ratio of the thickness of the first layer to the given index of the second layer is preferably from 1:2 to 1:200, preferably from 1:5 to 1:50, more preferably from 1:5 to 1:20.

Oxidoreductases the enzyme can easily be immobilized to prevent it from moving in the second layer.

The substrate, such as glucose, can be represented in the forms of hypoxia, including dissolved hydrated structure of the hydrogel present in the form of slowly dissolving solid or encapsulated in another structure for slow release.

It is preferable to adjust the bandage that she had an excess of substrate, so that the bandage was able to function when applying for the formation of hydrogen peroxide over a long period of time, for example, at least one hour, for example from 1 to 10 hours or more.

The combination according to the invention, is generally Packed set, which includes a combination of an aqueous solution and bandage comprising a source of hydrogen peroxide, as described above.

Components usually are in sealed waterproof bags.

The invention will be illustrated as an example and with reference to the following drawings, in which:

figure 1 is a graph showing the measured electric current with respect to time, showing the formation of hydrogen peroxide;

figure 2A is a graph showing curves of the destruction of the fungus T. Rubrum;

figure 2b is another graph showing curves of the destruction of the fungus T. rubrum.

Examples

A brief description of the experiment

Test discs were soaked in 50% serum, containing the her 10 7cells of the fungus to re-create the protein environment. They were placed on the gel spreading layer, containing 50% serum to simulate contact with the nail bed. The disks were dosaged aqueous solutions with horseradish peroxidase or lactoperoxidase, each set had a different dose levels of enzymes. Control disks were left without any peroxidase. The experiment was initiated by the imposition of forming hydrogen peroxide layered patches (see below) on the surface of most test drives and they were left in place for periods of time. Some discs left uncovered as an additional experimental controls to determine how well the cells of the fungi survive in the absence of treatment. At set time intervals disks, which are typical representatives were removed for sampling, and the number of surviving cells of fungi were identified according to standard methods. These experiments showed that hydrogen peroxide delivered layered gel patches that are able to kill cells of fungi, if left in place for several hours. However, the rate of destruction of the fungus greatly increased due to the additional presence of the enzyme peroxidase in contact with the fungus. The enzyme horseradish peroxidase was more powerful than lakhipur xidase, and the effect was generally increased with dose.

Example 1: the Design and evaluation of adhesive layers, transferring the hydrogen peroxide relative to the generation of hydrogen peroxide.

The generation of hydrogen peroxide (H2About2) was measured using electrochemistry (method time-consuming). The bandage was placed on a special sensor that measured the potential supplied to the electrodes in the presence of N2About2.

The dressing consisted of two layers: (i) a layer of hydrated hydrogel and (ii) the activation layer dry film containing glucose oxidase.

Preparation of layer 1: hydrogel plate was prepared as set forth below:

An aqueous solution of 20% sodium salt of 2-acrylamide-2-methylpropanesulfonic acid (Lubrizol Corporation, a 50% aqueous mother solution), 10% glucose (Fisher Scientific, air quality analysis), 0.1% zinc lactate (Aldrich) was prepared. PEG (polyethylene glycol) 700 diacrylate (Aldrich) was included as a cross-linking agent, and 2-hydroxy-2-methylpropiophenone (Aldrich) as photoinitiator. 6.5 g and 13 g of the solution were distributed into 10×10 cm Petri dish and exposed to UV light (100 mW/cm2within 20 seconds.

Preparation of layer 2: dry film was prepared as set forth below/

An aqueous solution of 25 wt.% solution of PVA (polyvinyl alcohol Gohsenol, code EG05P supplied the th Nippon Gohsei) was prepared. In addition, of 40.3 mg of glucose oxidase (Biocatalysts, 150000 unit/g) + 300 mg histidine (Sigma) + 150 mg citric acid (Fisher, quality analysis) + 75 mg of potassium iodide (Sigma) was dissolved in 2 ml of water quality analysis (Fisher). 30 g of 25% solution of PVA was mixed with a solution of glucose oxidase/ histidine/ citric acid/ potassium iodide and allowed to settle to remove any trapped air bubbles. The mixture was then dried with deposition at 50°C to obtain a dry film thickness of 40-45 μm.

Electrochemical analysis

Special 3-electrode sensor (working electrode, counter-electrode and the reference electrode) was used for analysis. The hardware and software Ezescan were purchased from Whistonbrook Technologies, Luton, UK. The electrodes were placed inside a Teflon box and placed in an incubator at 25°C. When applying 20 μl of 0.1 M solution of potassium chloride were applied to the end electrodes of the sensor. a 1.5×2 cm slices, layer 1 plate hydrogel (samples weighing 6.5 g and 13 g) were cut and placed on the solution KS1, ensuring uniform contact with the electrodes and that the bubbles did not catch between the gel and the sensor. The gels were covered to reduce evaporation. Voltage +950 mV was applied to the ends of the electrodes and recorded the generated current. If the constant background current was obtained, a 1.5×2 cm slices dry film applied on the surface the te layer 1 hydrogel plates. The generated current was recorded (see. figure 1)

Figure 1 shows that when the voltage +950 mV was applied to the layer 1 hydrogel plate on the sensor, there was very little of the measured background current, thus a slight interference from materials used in the preparation of the hydrogel, at a specified voltage. After applying the dry film of the enzyme (about 4000 seconds on the graph) is activated chemical process oxidase and is H2O2that rapidly diffuses through the layer 1 of the hydrogel to the electrode, where a marked increase in current. This clearly shows that the two-layer system forms an H2O2inside the two-layer system and delivers it to the contact surface. The current generated in relation to the main plateau of the graph (from 5000 to 13000 seconds)basically corresponds to approximately 0.1% N2O2(aq.). In addition, different thicknesses of layer 1 hydrogel receive very similar curves, except that a thinner gel (6.5 g of gel on the area of 10×10 cm) gave a significantly higher peak current (hence, the concentration of H2O2one hour of application (about 3000 seconds after activation). The measured index of N2O2then gradually reduced until the curves are not returned to the flat frequency response at about 40 000 seconds (n is IMEMO 11 hours) after activation.

Example 2: Preparation containing peroxidase primary samples as water solutions.

Suitable basic medium for peroxidase was prepared as follows: 50 mm sodium phosphate buffer pH 6-6,5 was mixed with 0.2% by weight surface-active agent Tween 20 (Sigma). For this purpose, the enzyme peroxidase was dissolved to obtain a final concentration of 100 µg/ml

The composition of the medium can be changed to provide different properties, if required. For example, the concentration and type of buffer salts can be modified to ensure that the range limits of the buffer action and to change the pH of the solution depending on the application and the optimum pH of enzyme used (e.g., peroxidase); the concentration and type of surfactant can be changed to provide different wetting characteristics, additional polymeric thickeners may be included to reduce the flow characteristics of the fluid (for example, to help prevent the draining of solution from the nail after application); the applied level of the enzyme can also be modified to allow increased or decreased mediated by peroxidase impact on the surface or inside of the processing structure. Additional additives may also be included to increase the effectiveness of dressings, for example antimicro the ing funds.

Example 3: system Test treatments against fungi of the present invention against Trichophyton rubrum in the model system.

In vitro model of the static diffusion in a plane layer was used according to the procedures described in "In vitro diffusion bed, 3-day repeat challenge 'capacity' test for antimicrobial wound dressings", J. Greenman, R. M. S. Thorn, S. Saad, A. Austin International Wound Journal (2006), 3, 322-329.

The inoculum for the test was obtained by emulsification biomass Trichophyton rubrum Mature superficial growth on agar of potato dextrose liquid medium Saboraud, extraction of the suspension, mixing on a vortex-mixer and then deposition of large particles from the solution. The resulting suspension was adjusted spectrophotometrically to obtain a standardized inoculum of approximately 107CFU/ml, which is sufficient density to allow detection of any fungicidal effects (reduction of >103CFU/ml is considered as evidence of a fungicidal effect).

100 μl of a certain monk late resuspendable in replications on cellulose disks on the surface of poly-AMPS test model (which is identical to layer 1 of the hydrogel, as described in example 1, but cast in the form of 25 g of the gel plate on the Cup 10×10 cm), resulting in the tested products can be applied here (figure 1). Experienced double-layer bandage. The layers were prepared as described in the ore 1. The enzyme lactoperoxidase or horseradish peroxidase was prepared by dissolving in water quality analysis to the desired concentration before use. It is believed that lactoperoxidase may exacerbate any antifungal effects of a new local treatment, and for some experimental systems cellulose disks were pre-treated with a solution of lactoperoxidase or horseradish peroxidase to establish different effects. The range of experimental conditions for test specimens and controls are shown in table 1.

Two-layer bandages were activated by connecting the two layers together. Hydrogel plate was placed in contact with the cellulose disk, and the dry film is then superimposed on the upper surface of the hydrogel plates. The subjects of the substrate were incubated at 28°C and set time intervals (0, 1, 2, 4 and 24 h); cellulose disks were removed, resuspendable in PBS, was doing a serial dilution and then spirally were sown on Saboraud dextrose agar. After incubation (5 days) number of dermatophytes, the survivors at different time points after exposure to treatment was determined by counting of colonies (CFU/disk). The results were graphically displayed and analyzed using GraphPad Prism (v.4).

Results

The results of the experiment presented at the Figo is e 2, and obviously, all test conditions showed significant antimicrobial effects, and 3 hours after exposure processing of viable cells could not be detected at any of the local treatments. Lactoperoxidase inside the disk shows amplification of the effects of local processing dependent on concentration, although the 1 μg/ml had no significant effect compared to only test treatment (figure 2A). Horseradish peroxidase inside the disk also increases the effects of local processing dependent on the concentration of way, where even the lowest concentration (1 µg/ml) has a significant effect (figure 2b). In the control samples, there was a slight decrease in the number of juvenile T. rubrum, showing that this body is not very well supported on analytical tablets fungal testbed, especially after 24 hours of incubation, although all test treatment showed significant effects compared with the control. Interesting was that the inactive control treatment offers some protection of the organism compared to the uncovered control at 24 hours.

Conclusions

As the results of the local processing has a significant antimicrobial effect on T. rubrum, reducing the number of viable cells to a level below the minimum point of detection for this system (2×102glue is OK) within 3 hours that shows fungicidal effect (>3 log-fold reduction). As peroxidase and horseradish peroxidase, increased the effects of local treatment, although it is obvious that the horseradish peroxidase is more efficient.

Table 1.
Experimental test and control conditions to determine the antifungal potential of various new treatments.
Control 1Uncovered disc
Control 2Inactive local processing
Test 1Active local treatment
Test 2Active local treatment + 1 µg/ml lactoperoxidase in drive
Test 3Active local treatment + 100 μg/ml lactoperoxidase in drive
Test 4Active local treatment + 500 μg/ml lactoperoxidase in drive
Test 5Active local treatment + 1 μg/ml horseradish peroxidase in drive
Test 6 Active local treatment + 100 μg/ml of horseradish peroxidase in the disk
Test 7Active local treatment+500 μg/ml of horseradish peroxidase in the disk

1. The combination of liquid water to soften the surface of the body of a human or animal with a fungal infection and a bandage comprising a source of hydrogen peroxide, for sequential application to the surface of the body, for use in the treatment of fungal infection of the surface of the body of man or animal.

2. The combination according to claim 1, where the surface of the body is the nail area, especially the nail area of a person.

3. The combination according to claim 1, where the aqueous liquid includes the enzyme peroxidase.

4. The combination according to claim 3, where the peroxidase includes lactoperoxidase, horseradish peroxidase, or a mixture.

5. The combination according to claim 3 or 4, where the concentration of the enzyme peroxidase in aqueous liquid is in the range from 1 to 1000 µg/ml

6. The combination according to claim 1, where the aqueous liquid includes surfactants and/or solvents.

7. The combination according to claim 1, where the bandage transmits water in the nail area in the application.

8. The combination according to claim 1, where the bandage includes a hydrated hydrogel material.

9. The combination according to claim 1, where the source of hydrogen peroxide is a means for obtaining hydrogen peroxide, comprising OK izoradoughty enzyme, the source of oxygen and a source of substrate for the enzyme.

10. The combination according to claim 9, where oxidoreductases the enzyme comprises glucose oxidase.

11. The combination according to claim 9 or 10, where the bandage includes separate first and second layers, the first layer includes oxidoreductases enzyme and is located near the outer parts of the bandage, the second layer includes the source substrate and located close to the inner parts of the bandage.

12. A method of treating infection of the body surface of a human or animal, comprising applying the combination defined in any of paragraphs. 1-11.

13. The method according to item 12, where the infection of the body surface is a fungal infection.

14. The method according to item 12, where the surface of the body is the nail area, especially the nail area of a person.

15. The use of combination defined in any of paragraphs. 1-11, for the manufacture of a medicinal product for the treatment of fungal infection of the surface of the body of man or animal.

16. The application indicated in paragraph 15, where the surface of the body is the nail area, especially the nail area of a person.



 

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3 tbl, 3 dwg

FIELD: medicine.

SUBSTANCE: group of inventions relates to medicine, namely to cosmetology, and can be used for treatment of skin aging. For this purpose, used is a medication, which contains a basic fibroblast growth factor (bFGF) as a single active ingredient, which is introduced intracutaneously or subcutaneously into the place of a scar or into the surrounding region, for instance, a keloid, a hypertrophic scar and a scar contracture; in addition, the medication is also intended for treatment of one or more types of skin aging, selected from the following list: skin wrinkles, sagging skin, rough skin, skin thinning and reduction of skin resilience and elasticity because of rupture of dermal tissues or reduction of functions of fibroblast cells, with skin aging being photoaging, and a value of a dose of the basic fibroblast growth factor (bFGF) constituting from 0.1 mcg to 1 mg per 1 cm2 of skin, which represents the treatment target.

EFFECT: inventions ensure significant reduction of wrinkles, improvement of the skin structure, as well as due to an increase of its turgor and an increase of the volume of the subcutaneous adipose cellular tissue.

6 cl, 4 ex, 10 dwg

FIELD: medicine.

SUBSTANCE: what is described is hydrogel composition containing sodium acrylate, a linking agent, biologically active substances, polyvinyl pyrrolidone, glycerol, propanediol, water, a catalyst agent and a radical polymerisation indicator in the following proportions, wt %: sodium acrylate 2.0-10.0, catalyst agent 0.045-0.48, linking agent 0.195-0.21, radical polymerisation indicator 0.045-0.06, glycerol 4.5-7.5, propanediol 3.0-10.5, biologically active substances 0-1.5, polyvinyl pyrrolidone 0.3-1.5, water - the rest. What is described is a surgical dressing containing a carrier with the hydrogel composition applied thereon.

EFFECT: higher efficacy of the hydrogel composition and surgical dressing thereof, lower labour intensity of the method for preparing the above composition.

10 cl, 1 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: dressing represents a knitted mesh fabric coated with a composition of a biocompatible film-forming polymer of polyvinyl pyrrylidone and drug preparations: iodine, novociane, carboxymethyl cellulose sodium salt, of the following formulation (mg/cm2): iodine 0.0282±20%; polyvinyl pyrrylidone (Mr 20000) 1.2±20%; novociane 0.426±20%; carboxymethyl cellulose sodium salt 1.6-1.9.

EFFECT: using the wipe provides antimicrobial, antiseptic, disinfecting, antifungal and antiprotozoal action ensured by the fact that a polyvinyl pyrrylidone matrix retains iodine in the wipe and promotes its release on the skin.

FIELD: medicine.

SUBSTANCE: postoperative period involves tamponage with Coletex-AGGDM wet tissue within middle nasal passages. The tamponage area is exposed to laser light in the infrared band at a wave length 0.95 mcm at the output power of 2-3 mWt for 4-5 min. After the exposure, the tissue is left for one day. The following procedures are performed with the tissues placed in the middle nasal passage within a natural fistula and on a mucous membrane of a hard palate in a projection of a maxillary sinus bottom. The tamponage area and skin in the projection of the maxillary sinus within an anterior sinus wall are exposed. 2-3 procedures are performed at the output power of 5-7 mWt for 7-10 min for one field. 4-6 procedures are performed at the power of 15-20 mWt for 3-10 min for each field. After the exposure is terminated after each procedure, the endonasal tissue is left for 6-8 hours.

EFFECT: method enables providing higher clinical effectiveness by antibacterial, antiseptic, anti-oedematous effects, recovering the ciliary transport function without injuring the ciliated epithelium; eliminating the postoperative complications, such as sinus bleeding and cicatrical deformity of the mucous membrane.

2 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to chemical-pharmaceutical industry and represents a copper-bearing cellulosic material possessing the fungicidal, bactericidal and deodorant properties, containing a cellulose matrix coated with copper particles prepared by chemical reduction of copper ions absorbed in the cellulose matrix, differing by the fact that the copper ions absorbed in the cellulose matrix are reduced in micelle solution of a cationic surfactant; the material contains copper and copper oxide nanoparticles with the size of 5-19 nm and has a composition as follows, wt %: cellulose matrix 99.5-98.0, copper nanoparticles 0.5-2.0.

EFFECT: said materials can find application in manufacturing sanitary products.

2 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to medicine. What is described is a method for preparing a transdermal therapeutic system based on poly(lactic-co-glycolic acid) involving dissolving poly lactide-co-glycolide and a pharmaceutically active substance in an organic solvent, mixing the prepared solution until dissolved completely, hot air drying until dry completely and having a constant weight to prepare a film, cutting the film into sections and packing, with the relation of lactide and glycolide varying within the range of 95:5 to 5:95 (versions).

EFFECT: preparing the transdermal therapeutic system based on poly(lactic-co-glycolic acid) that is biodegradable.

33 cl, 1 dwg, 3 tbl, 6 ex

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to traumatology and orthopaedics, enabling preparing a biologically active preparation of autoblood for enhancing neogenesis processes. The presented technique involves sampling whole blood, centrifuging, selecting a middle layer of plasma so that to avoid the erythrocyte ingress. The centrifuged platelet concentrate is frozen in a cold room at temperature below minus 1 C°, dried for at least three minutes within the temperature range of 2 C° to 52 C°; the lyophilisate is sterilised before use.

EFFECT: technique of platelet-rich plasma lyophilisation enables preserving the TGF PDGF VEGF factor viability min 1,5 months from the moment of blood sampling.

1 tbl

FIELD: medicine.

SUBSTANCE: what is presented is a boric coating containing a barrier material and an adhesive material. The barrier material and adhesive material are applicable for combined or sequential postoperative application on the mucosal tissue. Such application provides a multilayer film dressing containing a layer of the above barrier material and a layer of the above adhesive material contacting with the barrier material and mucosal tissue. The dressing is left unmoved in place for 48 hours after the postoperative application of the multilayer film on the mucosal tissue. The dressing is absorbed for 14 days. The multilayer film dressing has an external rim surrounding a tonsil bed and a concave centre shaped after the tonsil bed.

EFFECT: reducing or eliminating pain sensations following the mucosal operations, including tonsilectomy, adenoidectomy or other pharyngeal operations.

19 cl, 17 dwg, 3 ex

FIELD: medicine.

SUBSTANCE: invention refers to medicine, more specifically to dressings used in general surgery, traumatology, obstetrics, gynaecology, proctology, dentistry for closure and healing of wounds (including post-operative), bed-sores, ulcers, burns, complicated purulent and putrid infections with an evident purulo-necrotic layer. A wound and burn closure and healing dressing containing a wound or burn facing therapeutic layer consisting of a therapeutic and sorption agent that is a layer of paste-like gel of iron or aluminium gel with added graphite carbon at 1 g of the additive per 100 ml of the gel, of the thickness of 0.5 cm providing a sorption ability not less than 10 g/g.

EFFECT: preparing the wound and burn dressing possessing high antimicrobial activity and analgesic characteristics, improved sorption ability and atraumatic properties.

7 tbl, 23 dwg

Wound tissue // 2483755

FIELD: medicine.

SUBSTANCE: invention refers to medicine, to preparing a therapeutic and preventing agent for treating radioreactions accompanying the course of radiotherapy. A wound tissue contains a textile fibre-porous material with a viscose component coated with a polymer and a drug preparation introduced therein; the novel is the fact that it is suggested to use the textile material which is made of flax-viscose fibres in ratio of components of flax:viscose 40 - 70 and 60-30, respectively. The fibre-porous material is prepared by needle suturing and has area density 180 g/m2 - 260 g/m2. The coating polymer is sodium alginate, while the drug preparation is introduced in the concentration of 0.25-30 wt %. The drug preparation coating the suggested flax-viscose carrier is presented by poorly soluble preparations of Furaginum, metronidazole, 5-fluoruracil, antioxidant Mexidol, biologically active substances - propolis, sea buckthorn and blackberry extracts.

EFFECT: presented drug preparation has proved itself to be easy to use, enabling higher clinical effectiveness, increasing a prolongation time.

6 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to chemical-pharmaceutical industry and represents a sorption plate containing furacilin, differing by the fact that a process for making it involves using the additional excipient therapeutic powdered Kimmeridge clay (blue) Undorovskaya, chitosan solution, dimethylsulphoxide, glycerol, acetic acid and purified water in the following proportions (wt %): furacilin 0.75 - 1.5; dimethylsulphoxide 2.5 - 5.0; blue clay 7.5 - 8.5; 98% acetic acid 1.5 - 3.0; chitosan 2.5 - 4.5; glycerol (7.5 - 8.5); purified water up to 100.0.

EFFECT: invention provides making sorption plates for ensuring wound-healing, anti-inflammatory action in medical practice and higher sorption activity.

9 dwg, 1 tbl

FIELD: medicine.

SUBSTANCE: what is described is a biodegradable haemostatic therapeutic agent for control of bleeding, which provides co-immobilising ε-aminocapronic acid 50 mg, lysozyme 5 mg in distilled water 6.5 l for 3 hours at room temperature for dialdehyde cellulose 1 g at a degree of oxidation 12%. The material is pressed out and dried to residual moisture no more than 10% in the air in darkness. After having dried, the material is milled in a fine mill to particles having a size of 20 to 50 mcm. A rate of control of bleeding is 102 seconds. A time of total resorption is 10 days.

EFFECT: agent provides a high degree of hydrolytic destruction and a good haemostatic activity.

4 cl, 2 ex

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