Method for producing combination immunometabolic preparation with anti-infectious activity
SUBSTANCE: group of inventions refers to veterinary science and aims at normalising metabolic processes, stimulating immune system and blocking mechanisms of infectious process with a risk of endogenous infection activation. A method for producing a combination immunometabolic preparation with anti-infectious activity involves dissolving succinic acid and levamisole in demineralised water with formalin added. According to the other aspect of the invention, the immunometabolic preparation additionally contains polyethylene glycol. The ingredients are used in the declared ratio.
EFFECT: using this group of inventions provides producing an injection form of the immunometabolic preparation with anti-infectious activity.
2 cl, 3 tbl
The invention relates to veterinary medicine and relates to a method of obtaining a complex of the drug on the basis of succinic acid, levamisole, formalin and polyethylene glycol, which is intended to normalize the metabolism, stimulate the immune system and blocking mechanisms in the development of infectious process, at the risk of activation of endogenous infection, including for the treatment of infectious diseases of viral and bacterial etiology.
A method of obtaining drug "amber biostimulator" to increase the resistance of the organism of animals (RF patent No. 2303979, 2007). The combination of succinic acid and antiseptic stimulant Dorogov second fraction (SDA-f No. 2) provides the ability to get the drug with pronounced immunometabolic activity. However, succinic acid will neutralize the antiseptic activity of the SDA-f No. 2, which ensured a high alkaline reaction.
As the closest analogue of the selected method of obtaining immunotropic antiseptic drug for the treatment and prevention of infectious animal diseases (patent RF №2361579, 2009). This drug contains succinic acid, SDA-f # 2 and formalin (formal amber biostimulant).
In addition immunometabolic drug has a pronounced antiseptic activity. But not what atcom this drug is used as immunostimulant DCA-f No. 2. ASD f-No. 2 obtained by high temperature sublimation of meat and bone meal. The resulting product has a highly variable chemical composition, which complicates its use as a medicinal substance. Also used for the manufacture of the SDA-f No. 2 raw - meat-bone meal can contain high amounts of heavy metals, including radionuclides that accumulate during the life of animals in the bone tissue.
The task of the invention to provide injectable form immunometabolic drug with anti-infective activity.
Serves as immunostimulant to include in the proposed standard drug substance of levamisole.
Levamisole has long been known and are widely used in medicine to stimulate the cellular immune system. In veterinary medicine it is used as an anthelminthic drug. However, levamisole has a strong side, mainly convulsive effect on the body.
What's new is that the integrated product includes in its composition as immunostimulant levamisole; stabilizer and prolongator - polyethylene glycol (PEG).
The combination of metabolica (succinic acid), immunomodulator of levamisole and formalin is quite acceptable due to the different mechanism of action.
Formalin is about the fully exceptionally high antiseptic activity. So, in a concentration of 1:6000 it stops the growth of the typhoid bacilli, and absolutely low concentration of 1:30000 stops the rot broth.
According to Lutcavage NR. (1997), intramuscular 0.2% formalin allows to reduce the virulence of the pathogen virus transmissible gastroenteritis and simultaneously to stimulate specific immune protection. Formaldehyde has a protective effect on the living cells of organs and tissues, inhibits the damaging processes of lipid peroxidation, is present in a normally functioning tissue, can prolong the time of death, heart and brain.
The above effects have formed the basis for inclusion of formaldehyde in the composition of our previously developed immunometabolic drug - amber biostimulant (RF Patent No. 2303979).
The inclusion of the drug PEG provides stability and prolongation of action. It is very important that the glycols with a molecular weight of up to 600 can be mixed with water in any relationship and have a pronounced low toxicity. In pharmacology, the glycols are considered practically non-toxic substances. Currently, the glycols are widely used in pharmaceutical, cosmetic, food industry.
In preliminary experiments nabilah mice was established, that intraperitoneal injection in a volume of 0.25 ml, comprising a standard 10% solution of levamisole, which added a 1% succinic acid, caused a pronounced low toxicity on the body. An even more pronounced reduction of side effects was observed when adding to 1.5 and 2% succinic acid 10% solution of levamisole. In turn, the composition comprising from 1% to 2% succinic acid and 2-3% of levamisole, did not cause any side effects on the body with three times with an interval of 24 hours intraperitoneal administration. Moreover, experimental animals (white mice) became more cheerful, and they had improved appetite.
This object is achieved by inclusion in the composition of the complex preparation of succinic acid, levamisole, formaldehyde and glycol in the following ratio, wt.%:
Succinic acid is 1.0 to 1.5
Levamisole - 2-3
Formalin - 0,3-0,4
The glycol - 7-8
Example 1 of the method of sample preparation of the drug.
For preparation of the comprehensive drug is used in 950 ml of demineralized water, which when heated successively dissolved 10.0 g of succinic acid and 20 g of levamisole (1st version); 15 g of succinic acid and 30 g of levamisole (2nd option). In the cooled to 40-50°C. the composition of each sample was added 10.0 ml of formalin (39% concentration). Add in the solutions is poliatilenglikola to 7-8% increased their density. The total volume was brought to 1000 ml by adding demineralized water. The resulting solution had a pH of 4.5 to 4.7.
In a similar manner there were prepared samples of similar compositions, but without the addition of polyethylene glycol.
Sterilization by autoclaving did not change the transparency of the solution, which testified to the full compatibility of the components.
Test safety. Experiments conducted on white mice. Daily, for 3 days intraperitoneal injection in a volume of 0.25 ml of the above samples of the test drug was not accompanied by the suppression of the General condition experimental animals. On the contrary, the introduction of levamisole in similar concentrations was accompanied by a severe depression lasting from 20 to 30 minutes to 2-3 hours.
The effectiveness of the proposed drug tested in tests of anti-infective and immunometabolic actions.
The test of protective properties
Object for the experiments served as white mice with an average weight of 18-20 g In preliminary experiments on white mice were determined by the minimum lethal dose of cultures of E. coli and S. cholerae suis. The minimum LD100for E. coli was 500 thousand microbial bodies (M.L.) in 1 ml, and for S. suis - 400 thousand M.L.
Conducted two series of tests of two samples of the integrated product, prepared as described in example otlichayushchayasya acid and levamisole. Each sample was tested in two versions: with prolongator PEG without PEG.
The first series. This series was tested the drug, including: 1% succinic acid; 2% of levamisole; 0.3% formalin; 7% of polyethylene glycol (PEG) and without PEG. The drugs were administered intraperitoneally in a volume of 0.25 ml.
The procedure of the experiment was the following. Mice of the first group of trial medication (without PEG) in a volume of 0.25 ml was introduced for 6 hours before infection with E.coli culture. The mice of the second group were injected similarly, the test drug with PEG and after 6 hours spent infection. Mice of the third and fourth groups were first infected with E.coli culture. After 6 hours the mice 3 groups was introduced trial medication without PEG, and mice 4 groups - the test drug with PEG. Control group mice were injected with saline for 3 hours before infection. The results of the study (Protective activity immunometabolic drug when modeling deadly infectious process culture of E. coli in a dose of 1 LD100) are presented in table 1.
Observation of the experimental mice was carried out within three days. During this time period, all mice in the control group fell. At autopsy, they were revealed changes characteristic of acute septic process.
When observation of the mice that were treated with the drug in combination with PEG showed h is about the survival rate was significantly higher. So, in group 4, the survival rate amounted to 85.7% versus 42.9% in relation to animals 3 groups.
The results of the first experimental experience testified to the fact that the metabolic composition has a certain protective effect when knowingly infecting mice with a lethal dose. Protective effect in long periods from infection significantly increased when the drug PEG.
The second series. This series was tested in the drug composition, comprising: a 1.5% succinic acid; 3% of levamisole; 0.3% formalin; 7% of polyethylene glycol (PEG) and without PEG. The drugs were administered intraperitoneally in a volume of 0.25 ml for 6 hours before infection. The results of the research (Protective metabolic activity of the drug in the simulation of mixed infection in white mice by culture of E.coli (2LD50and S. cholerae suis (2LD50) are presented in table 2.
Obtained in two series, the results indicate that the test drug has a definite anti-infective action. While anti-infective activity is expressed increases in distant time periods after infection, when included in the composition of the drug PEG.
The study of metabolic activity
In this series of experiments was used immunometabolic the drug, including: 1% succinic acid; 2% of levamisole; 0.3%formalin; 7% of polyethylene glycol and without PEG. The object of the experiments was suckling piglets-lipotropic 6-7 days of age, had a body weight of up to 1000
In accordance with the principle of analogs selected for the experiment pigs were divided into 2 groups. The piglets of the experimental group (n=8) was injected intramuscularly immunometabolic composition without PEG with multiplicity every 7 days. The amount of administered drug was 1.0 ml - 1,5-2,0-2,5 g In pigs of the second group (n=8) in the same manner and amount used the drug with PEG.
When the clinical observation found that already at day 2 after the introduction of the state of Guinea pigs of both experimental groups was significantly different from their peers in the control group. First, they became more cheerful; secondly, they have become more pronounced nutritional needs. These differences in piglets from the experimental groups relative to individuals from the control group increased with each passing day.
Study of the effect on hematological and immunobiochemical state of Guinea pigs spent on 7-14-21 and 28 days. The research results are reflected in table 3.
Based on the experience and results of the study for Hematology and immunobiochemical state of Guinea pigs made the following conclusion.
Parenteral administration of the test drug on the pigs-hypotrophic the x provides a pronounced stimulation of metabolic processes, what a favorable effect on the acceleration of their physiological development and the elimination of state of immunodeficiency.
|Group||Medication||Processing||The death of mice after (hours)||Survival|
|1 (n=7)||Immunometabolic drug without PEG||6 hours before infection||1||3||-||3||42,8|
|2 (n=7)||Immunometabolic drug +PEG||6 hours before infection||1||2||-||4||67,0|
|3 (n=7)||Immunometabolic the drug without the AG||After 6 hours after infection||2||2||-||3||42,9|
|4 (n=7)||Immunometabolic drug +PEG||After 6 hours after infection||1||6||85,7|
|5 (n=5)||Saline||3 hours before infection||3||2||-||-|
|Group||Medication||The death of mice after (hours)||Survival|
|Immunometabolic drug without PEG||2||1||2||-||5||50|
|2 (n=10)||Immunometabolic drug +PEG||1||2||7||70|
|3 (n=5)||Phys. solution||2||1||2||0||0|
1. The method of obtaining comprehensive immunometabolic drug with anti-infective activity, including dissolved in demineralised water succinic acid, adding formalin solution, characterized in that as immunostimulant use levamisole in the following ratio, wt.%:
2. The method of obtaining comprehensive immunometabolic drug with anti-infective activity, including dissolved in demineralised water succinic acid, adding formalin solution, characterized in that as immunostimulant use levamisole and additionally enter the glycol in the following ratio, wt.%:
|The polyethylene glycol||7-8|
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to the chemical-pharmaceutical industry and represents a method of obtaining a complex preparation for the application in veterinary, possessing immunomodulating and antiseptic properties, which includes mixing succinic acid, levamisole and formalin in distilled water with the following component ratio, wt %: levamisole - 3.0%-3.5%; succinic acid - 2.0%-2.5%; formalin - 0.3%-0.5%, distilled water - the remaining part; sterilisation of the solution by autoclaving is performed in a mode of 1 atm for 20 minutes.
EFFECT: elaboration of the method of obtaining the complex preparation for the application in veterinary, possessing immunomodulating and antiseptic properties.
FIELD: medicine, pharmaceutics.
SUBSTANCE: claimed group of inventions relates to medicine, namely to therapy and immunology, and deals with treatment of autoimmune diseases. For this purpose 3-(5-(4-(cyclopentyloxy)-2-hydroxybenzoyl)-2-((3-hydroxy-1,2-benzisoxazol-6-yl)methoxy)phenyl)propionic acid or its salt is introduced in the form of a combination or a pharmaceutical composition with one or more of TNFα inhibitors.
EFFECT: invention provides efficient treatment of autoimmune diseases due to synergic action of the said components.
6 cl, 1 ex, 1 tbl
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to the pharmaceutical industry, namely to a personal preparation from earthworms for treatment of diabetes. The method of obtaining the personal preparation from earthworms for treatment of diabetes includes placement of sexually mature earthworms in a medium, consisting of organics, which additionally contains a solution of a patient's urine, after that, the sexually mature earthworms are removed and for 3 months each 7-10 days fresh organic food is added to the remaining mass, after that, the sexually mature earthworms are separated, placed in a reservoir and sprayed with an alcohol solution, mixed, poured with the alcohol solution and placed into dark place under the specified temperature, with the solution being mixed daily, with further separation of the extract from the sediment. The personal preparation for treatment of diabetes.
EFFECT: application of the described above preparation increases efficiency of diabetes treatment.
3 cl, 3 dwg, 1 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine, more specifically to a pharmaceutical composition possessing antithrombotic, thrombolytic, immunomodulatory, anti-inflammatory action, normalising lipid and carbohydrate metabolism, more specifically to the pharmaceutical composition of the substance Pijavitum (hereinafter referred to Pijavitum) made from lyophilised medicinal leech. The above pharmaceutical composition is presented in the form of an enteric coated tablet.
EFFECT: coating prevents the active ingredients of Pijavitum from destruction under action of the enzymes and acid medium of the stomach.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to a new antineoplastic drug representing 2-isobutyl-4,6-dimethyl-5-oxypyrimidine of the general formula I specified below. The drug may be used for an adjuvant antineoplastic immune therapy. The invention also refers to a method for preparing 2-isobutyl-4,6-dimethyl-5-oxypyrimidine. According to the method, isocaproic acid amide reacts in ice-cold acetic acid with chloracetyl acetone to form the respective oxazole which reacts with aqueous ammonia which leads to preparation of the above compound.
EFFECT: what is disclosed is a manifested tumour growth inhibition with no signs of the toxic effect on somatic characteristics of tumour-carrier mice.
3 cl, 9 tbl, 6 ex
SUBSTANCE: what is presented is a method for simulating a delayed hyperresponsiveness to mycobacteria bovis. Avirulent mycobacteria bovis of the strain BCG are administered intracutaneously to albino guinea pigs produced by consanguineous mating of brother x sister (F2); that is followed by forming a group (groups) of animals showing various extents of an inflammatory response in reaction to the intracutaneous administration of mycobacteria bovis of the strain BCG 30-35 days later.
EFFECT: method is effective in studying the mechanisms of the delayed hyperresponsiveness and assessing the efficacy of antituberculosis agents.
2 tbl, 5 ex
SUBSTANCE: agent, having adaptogenic and immunomodulating activity, containing medicinal marigold flower heads; rhaponticum carthamoides root and rhizome; horse-heal rhizome; nutmeg fruit; cardamom fruit; calamus root; sweet weed root; ginger rhizome; knotgrass; cinnamon bark; pomegranate; long red pepper; juniper fruit; leather bergenia black leaves; chitosan, taken in a defined amount.
EFFECT: agent has marked adaptogenic and immunomodulating activity.
SUBSTANCE: group of inventions relates to medicine, in particular to gastroenterology, and deal with treatment of ulcerative colitis and Crohn's disease. Method of treatment includes introduction into organism of therapeutically efficient quantity of attaching cells from placenta, cultivated in such a way as not to differentiate into adipocytes or osteocytes. Also claimed is application of said cells for obtaining medication, intended for treatment of ulcerative colitis or Crohn's disease. Claimed produced product for treatment of ulcerative colitis or Crohn's disease includes in packing form pharmaceutically efficient quantity of said cells.
EFFECT: inventions ensure essential reduction of inflammatory process in colon in modelling of said diseases, as well as due to pathological mechanisms of treatment in addition to T-lymphocyte suppression.
31 cl, 5 ex, 12 tbl, 16 dwg
SUBSTANCE: invention refers to medicine, particularly to paediatrics and neonatology, and can be used for treating small premature infants at the hospital stage of developmental care. A therapeutic complex comprises administering a probiotic preparation into the newborns. The preparation is presented with a liquid probiotic containing E.faecium L3 109 CFU in 1 ml. If the enteral nutrition volume of the newborn is 5 ml or more, this preparation is orally administered in a dose of 0.5 ml 3 times a day for 14 days.
EFFECT: method is effective in children with a very low body weight, promotes normalising the intestinal microflora and reducing a rate of manifestations of infectious complications.
2 ex, 3 dwg, 3 tbl
SUBSTANCE: group of inventions relates to biotechnology and medicine. Disclosed is a polysaccharide which is isolated from the Bifidobacterium infantis NCIMB 41003 strain and has the structure [-β(1,3)-D-GalpNAc-β(1,4)-D-Glcp-]n, where said disaccharide unit repeats n times, which yields a polysaccharide with molecular weight greater than 100000 Da. The polysaccharide exhibits immunomodulating activity and is used in preparing medicinal agents for treating or preventing undesirable inflammatory activity, undesirable gastrointestinal inflammatory activity, rheumatoid arthritis and autoimmune disorders.
EFFECT: pharmaceutical composition for treating and preventing inflammatory disorders and a food product containing the isolated polysaccharide are disclosed.
9 cl, 6 dwg, 3 ex
SUBSTANCE: treating a wound surface with dioxidine is followed by an infrared laser light with a permanent magnetic field not earlier than 5 days after the operation. Magnetic induction intensity is within the range of 20-50 mT; a laser pulse repetition frequency is within the range of 80 Hz, and a power is 0.25-0.5 W. The whole postoperative area is subject to the daily distant labile exposure to a defocused beam at 0.5 cm for 30-60 seconds. That is followed by applying tissues with hypertonic solution 3 to 5 times a day; the therapeutic course is 10-15 procedures.
EFFECT: method enables providing higher effectiveness and reducing a length of treatment ensured by the integrated exposure to the antibacterial agents and magnetic laserophoresis in the presented regimen, preventing developing postoperative complications.
SUBSTANCE: group of inventions refers to medicine, namely to medical microbiology, and can be used for treating intestinal yersiniosis, or pseudotuberculosis, or salmonellosis experimentally. That is ensured by simulating one of the above pathologies in experimental animals followed by oral administration of therapeutic preparations in therapeutically effective doses with controlling the content of pathological microflora for a period of time providing elimination of the disease. Stimbifid or a culture fluid of lactic acid bacilli is administered orally as the therapeutic preparation into each animal for the first 2 hours after infection once a day as a monotherapeutic preparation promoting the colonisation resistance of the intestinal mucosa to an agent. Stimbifid is administered into each animal in a dose of 13 mg for 5 days from the moment of the infection, while the culture fluid is administered in an amount of 0.2 ml throughout 7 days from the moment of the injection. What is also presented is a method for preparing an agent for treating intestinal yersiniosis, or pseudotuberculosis, or salmonellosis experimentally.
EFFECT: group of inventions provides a high percentage of pathology elimination by using the prebiotic preparation and the liquid probiotic complex with no accompanying negative consequences.
4 cl, 3 tbl, 3 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to methods of treating or relieving severity of disease in patient, where disease is selected from mucoviscidosis, hereditary emphysema, chronic obstructive pulmonary disease (COPD), "dry eye" disease. Methods include introduction of effective amount of N-(5-hydroxy-2,4-di-tert-butylphenyl)-4-oxo-1H-quinoline-3-carboxamide or pharmaceutical composition, containing said compound, to patient.
EFFECT: treatment of relief of disease severity in patient, where disease is selected from mucoviscidosis, hereditary emphysema, chronic obstructive pulmonary disease (COPD), "dry eye" disease.
16 cl, 15 tbl
FIELD: medicine, pharmaceutics.
SUBSTANCE: claimed invention provides pharmaceutical compositions, which include an effective quantity of ceftaroline fosamile or its pharmaceutically acceptable salt and an antibacterial agent or its pharmaceutically acceptable salt. The antibacterial agent is selected from a group, consisting of meropenem, piperacillin plus tazobactam, amikacin and astreonam.
EFFECT: compositions by the invention produce a synergistic effect on bacteria strains, are used in treatment of bacterial infections.
7 cl, 2 dwg, 17 tbl, 3 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: in formula R1 is H or (1-6C alkyl); R2 represents NRbRc, (1-4C)alkyl, (1-4C)fluoroalkyl, CF3, (1-4C)hydroxyalkyl, -(1-4Calkyl)hetAr1, -(1-4Calkyl)NH2, -(1-4C alkyl)NH(1-4Calkyl), -(1-4Calkyl)N(1-4Calkyl)2, hetAr2, hetCyc1, hetCyc2, phenyl substituted where applicable by NHSO2(1-4Calkyl) or (3-6C)cycloalkyl, substituted where applicable by (1-4C alkyl), CN, OH, OMe, NH2, NHMe, N(CH3)2, F, CF3, CO2(1-4C alkyl), CO2H; C(=O)NReRf or C(=O)ORg; Rb is H or (1-6C alkyl); Rc represents H, (1-4C)alkyl, (1-4C)hydroxyalkyl, hetAr3 or phenyl, wherein the above phenyl is substituted where applicable by one or more substitutes independently from halogen, CN, CF3 and -O(1-4C alkyl); Re represents H or (1-4C)alkyl; Rf represents H, (1-4C)alkyl or (3-6C)cycloalkyl; Rg represents H or (1-6C)alkyl; X is absent or represents -CH2-, -CH2CH2-, -CH2O- or -CH2NRd; Rd represents H or (1-4C alkyl); R3 represents H or (1-4C alkyl); and n is equal to 0-6. The radical values NRbRc, Y, hetAr1, hetAr2, hetAr3, hetCyc1, hetCyc2, NReRf, R4 are specified in the patent claim. The invention also refers to a pharmaceutical composition containing the above compounds, to a method of treating Trk kinase mediated diseases and conditions, such as pain, cancer, inflammation, neurodegenerative disease, Typanosoma cruzi infection, osteolytic disease, and to a method of preparing the above compounds.
EFFECT: invention refers to new derivatives of pyrazolo[1,5-a]pyrimidines possessing an inhibitory activity on tropomyosin-related kinases (Trk).
42 cl, 1 tbl, 105 ex
FIELD: veterinary medicine.
SUBSTANCE: method of emergency prevention and treatment of brucellosis, comprising simultaneous intramuscular administration of antibiotic resistance version of the vaccine strain of Brucella and antibrucellar preparation, characterised in that as the particular preparation the cyprolet- resistant version of strain of Brucella B.abortus 82 CR is used at a dose of 1.109 m.c. , and as the antibacterial preparation the ciprofloxacin (cyprolet) is used which is administered at a dose of 1 and 3 mL/kg, 2 times per day for 5-7 days. The cyprolet- resistant version of Brucella B.abortus 82 CR is obtained by growing a stock culture of Brucella B.abortus 82 on MPGGA, containing 5, 10, 20 mg per 1 ml of medium. Prevention and treatment of brucellosis is carried out 1-10 days prior to the period of greatest risk of infection and in the period of greatest risk of infection.
EFFECT: method increases effectiveness of treatment and prevention of brucellosis due to interference of avirulent strain of Brucella in relation to brucellosis pathogen, induction by cyprolet- resistant version of vaccine strain of mediators of immunogenesis - activators of phagocytosis-cytokines, powerful antibacterial agent cyprolet, which together provide the suppression of reproduction of microbial DNA and death of parasite.
3 cl, 8 ex
FIELD: veterinary medicine.
SUBSTANCE: method comprises hyperimmunisation of bulls-producers with inactivated antigens C1. perfringens, comprising toxoids and somatic antigens of bacteria of serotypes A, C and D in the culture medium with the following ratio of components per 1 litre of antigen: toxoid and somatic antigen of strain No 28 (type A) in the culture medium with a concentration of 1012 · (9-10) m.c. in 1 cm3, cm3 - 300.0-350.0; toxoid and somatic antigen of strain No 392 (type C) in the culture medium at a concentration of 1012 · (9-10) m.c. in 1 cm3, cm3 - 300.0-350.0; toxoid and somatic antigen of strain No 213 (type D) in the culture medium at a concentration of 1012 · (9-10) m.c. in 1 cm3, cm3 - 300.0-350.0; formalin, cm3 - 4.0-5.0. The additional hyperimmunisation of bulls-producers is carried out with inactivated antigens of E.coli, comprising somatic and adhesive antigens K99, A20 in saline, TL-, TS-toxoids of E.coli in the culture medium in the following ratio of components per 1 litre of antigen: somatic and adhesive antigen of strain KV-1 (K99) in saline with a concentration of 1012 · (14-15) bln.c. in 1 cm3, cm3 - 300.0-350.0; somatic and adhesive antigen of strain PZ-3 (A20) in saline with a concentration of 1012 · (14-15) m.c. in 1 cm3, cm3 - 300.0-350.0; formalin, cm3 - 4.0-5.0; thermostable and thermolabile toxoids of E. coli strains KV-1 and PZ-3 in the culture medium (DPR) 1:8-1:16, l - to 1. Hyperimmunisation of bulls-producers is carried out four times by subcutaneous injection in the neck region on one side of the antigen dose C1. perfringens and in the neck region on the other side - E.coli antigen dose, according to the following scheme: on the first day - 8 cm3, after 14 days - 10 cm3, after 28 days - 15 cm3, and after 42 days - 20 cm3 from blood, then serum is separated and preserved.
EFFECT: invention with the further intake enables to obtain the hyperimmune serum, which provides treatment and prevention of mixed infection - anaerobic enterotoxaemia and colibacillosis diarrhoea of calves.
5 tbl, 7 ex
SUBSTANCE: invention refers to veterinary science and aims at preventing and treating the porcine respiratory disease complex PRDC. What is declared is using a recombinant protein ORF2 PCV2 or an immunogenic composition containing the recombinant protein ORF2 PCV2, for preparing a therapeutic agent for preventing and treating the porcine respiratory disease complex (PRDC) caused by Mycoplasma hyorhinis and/or a PRDC-associated clinical symptom of a notable increase of a death rate in the middle to late feeding phase caused by PCV2, and at least one another pathogen causing PRDC in animals, wherein one pathogen causing PRDC is specified in a group containing PRRSV, Mycoplasma hyopneumoniae, Bordetella bronchiseptica, swine influenza virus, Actinobacillus pleuropneumoniae, Mycoplasma hyorhinis. Streptococcus suis and/or Pasteurella multocida.
EFFECT: invention is high-effective for treating and preventing the porcine respiratory disease complex PRDC.
2 dwg, 3 tbl, 3 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: present group of inventions refers to medicine, namely to neurology, and concerns treating infectious diseases accompanied by neurotoxic disorders. That is ensured by administering a pharmaceutical composition containing an activated-potentiated form of human gamma-interferon (IFN-γ) antibodies and an activated-potentiated form of brain-specific protein S-100 antibodies.
EFFECT: drug preparation provides effective treatment of the above pathology ensured by the synergetic action of the ingredients of the composition.
9 cl, 4 dwg, 1 tbl, 2 ex
SUBSTANCE: invention relates to medicine, namely to gynaecology, reflexotherapy and pelotherapy. A method includes carrying out a course of antibacterial and/or antiviral therapy, which is started on 5-7 day of a menstrual cycle. From 5-7 day of the following menstrual cycle a course of pharmacopuncture is performed by introduction of homeopathic preparations into acupuncture points (AP). On 1, 3, 5, 7, 9, 11 and 13 days of the course Traumel C is introduced into points E36 (2), V31 (2), V32 (2), V33 (2), V34 (2). On 2, 4, 6, 8, 10, 12, 14 days of the course Ovarium compositum is introduced in AP Rp6 (2). Simultaneously with the course of pharmacopuncture or starting from 5-7 day of the following menstrual cycle a course of pelotherapy is carried out. Introduction of gel, based on the Dead Sea mud, is performed rectally for 30 minutes, 1 time per day.
EFFECT: method ensures recovery of the two-phase menstrual cycle due to normalisation of endometrium and vagina biocenosis, improvement of local immune and vegetative status, increases duration of remission.
4 cl, 2 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to application of quaternary ammonium compounds, such as benzalkonium chloride, bromide, or iodide or benzethonium chloride, bromide or iodide as medication for prevention precipitation of pradofloxacin of formula
or its pharmaceutically acceptable salt or hydrate into sediment from solutions, containing pradofloxacin in amount 0.1-15% (wt/vol), bivalent cations of metals, such as cations of alkali earth metals in amount 0.5-5% (wt/vol), water and pH regulator - the remaining part, as well as medication with antibiotic action, which contains in dissolved form: pradofloxacin in amount 0.1-15% (wt/vol), quaternary ammonium compound, such as benzalkonium chloride, bromide, or iodide or benzethonium chloride, bromide or iodide, bivalent cations of metals, such as cations of alkali earth metals, in particular Mg2+, in amount 0.5-5% (wt/vol), water and pH regulator.
EFFECT: medication preserves stability for 5 years.
5 cl, 2 tbl, 8 ex