Combined graft of dermal matrix with mesenchymal multipotent stromal cells, method for preparing it and method for wound healing using it
SUBSTANCE: combined graft (CG) represents a dermal matrix (DM) prepared of a donor layer with self-specific multipotent mesenchymal stromal cells (MMSC) colonised on the surface thereof to the number of not less than 750-1500 thousand per 1 cm2. The group of inventions also refers to a method for preparing the combined graft involving bone marrow sampling from the patient, preparing a MMSC culture, applying a MMSC suspension in the concentration of not less than 1 million/ml on the DM surface at 1 million MMSC per 5 cm2 of the DM.
EFFECT: skin and soft tissue wound healing by accelerating regeneration processes.
The invention relates to medicine and biotechnology and can be used to treat a wide traumatic defects in surgery, traumatology and combustology.
The main method of treatment of extensive traumatic defects is the primary surgical treatment (PHO) wound with subsequent delayed skin-plastic surgery for closure of the skin defect [Alekseev A.A., Sutugin A.M., Kuznetsov V.A. Modern strategy and tactics of treatment of burns, thermal burns and extensive purulent wounds. Postgraduate education at the present stage. M. - 2000. - c. 277-284].
For wound closure used autodermoplasty (ADP) split flap. However, the results of the ADP in purulent surgery remain unsatisfactory, due to incomplete regeneration of the skin and scar formation [Gubin M.A., Aktov V.N., Lakatos C.O Early reconstructive plastic surgery in the treatment of patients after thermal injury of the head and neck: the Man and his health. - Kursk. - 2000. - Vol, 3. - C-84]. We emphasize that, when ADP is required to get a skin flap from the intact surface of the patient's body, which creates additional difficulties.
Development of cellular technologies opens new possibilities in the treatment of wounds using a variety of cells, including mesenchymal multipotent stromal cells (MSC). In clinical PRA is tick use of allogeneic or autologous cells. The type of cells used is determined by the alleged tactics of treatment, due to the nature of the pathological process. To stimulate regeneration at the expense of the resident progenetor cells used bioactive factors secreted from autologous or allogeneic cells when destruction in situ or in vitro. In particular, the effectiveness of this method was demonstrated in the treatment of borderline burns when you saved the source of regeneration of the skin, to stimulate that use alleyball.
When the deficit resident progenetor cells perform their autotransplantation of intact areas, for example, bone marrow (KM).
The closest technical solution adopted for the prototype, is a method of treating Ozegovich RAS IIIa degree, developed in the Institute of JV to them. N.V. Sklifosovsky, which consists in imposing on burn wound dressings containing alleyball [Patent No. 2320349 "Method of treatment Ozegovich wounds" from 27.03.08]. To speed up the process of regeneration stimulated preserved in the derivatives of the skin progenetor cells. Stimulation of cells perform appliqué on the biological wound dressings consisting of perforated hydrophobic silicone polymer base, coated with human collagen type I, attached to allofibroblasts cell line M-22, polucen the mi at the Institute of poliomyelitis and viral encephalitides. M.P. Chumakov. The dressing is made in terms of cultural block. On button located on the bottom of the Petri dishes hydrophobic transparent perforated silicone film "Carbosil-P coated with human collagen type I, transferred allogeneic diploid fibroblast cell line M-22 passage No. 15-25. The planting density is 5.0 and 7.5×104cells on 1 cm2. Cultivation was performed at 37°C in atmosphere containing 5% CO2and 100% humidity. As a culture medium use medium Needle MEM containing 15% serum of cow embryos and α-glutamine. Within 18-24 hours on the surface of the polymer base fibroblasts form a monolayer. On the organosilicon film "Carbosil-P" transfer of alloparental on the wound.
Transplantation alloparental, according to the method prototype, is from time of receipt until 6 days after injury. Optimal is the imposition of this armband for admission of the patient to the admitting office. Before transplantation conduct thorough washing of burn wounds, remove from the wound surface of a foreign body, desquamated epidermis, the imposition of fibrin. Treat the wound with an antiseptic solution, burns IIIa extent impose a bandage with allofibroblasts that capture the wet-drying gauze bandage.
JV is the FDS prototype treatment of burn wounds has the following disadvantages: limited indications for use only when the edge burns IIIa degree; a small number allowable on 1 cm2headbands and short life expectancy in the wound (no more than 2-3 days), which does not allow efficient enough to stimulate regenerative processes in deep wounds where there is no resident stem cells.
Autologous cells in the wound retain their viability for a long time, being able to replace the resident stem cells and stimulating regeneration. DM is the optimal biocompatible carrier, in comparison with the silicon film.
Thus, for the treatment of patients with extensive traumatic soft tissue defects there is a need to develop technologies create a new composite graft with autologous MSC applicable at different stages of treatment.
The technical result is achieved by the use of technology for combined transplant: dermal matrix with autologous MSC providing wound healing of skin and soft tissues, due to the acceleration of regenerative processes.
The method of obtaining a combined transplant (KT)
The technology of CT involves several steps and is carried out in conditions of cultural block to ensuring sterility and biological security of the obtained graft.
1 this is the Receipt of the dermal matrix
Dermal matrix made of split human skin, harvested from a donor cadaver, thickness up to 1 mm Fence of the skin flap is made in the operating room using a disk dermatome in compliance with the rules of asepsis and antisepsis.
The manufacturer of the dermal matrix is divided into 3 phases: separation of dermis and epidermis, devitalizate dermis, ensuring the biocompatibility of the matrix. DM is a plate of white or light beige color, consisting of collagen and elastin fibers without cells and cellular structures.
stage 2. Obtaining autologous MSC bone marrow (KM).
The patient under local anesthesia or General anesthesia in the operating conditions in compliance with the rules of asepsis and antisepsis of the wing of the Ilium prepare KM. The trocar to pierce the soft tissue, the dotted line in the bone. The trocar attach a syringe with a volume of 20.0 ml, containing 5,000 IU of heparin, which gather Malaspina. Prepare 20-40 ml KM. Not later than 2 hours Malaspina take in the culture unit. In the culture unit of KM on the gradient density Linfoot p=1.077 distinguish nucleated cells. Cells are placed in culture flasks to obtain pesticidesin cells. Adherent to plastic cells cultured 3-4 passage in the environment DMEM with the addition of 10% bovine fetal the first serum to obtain culture MMSC. After the third passage in suspension MSC determine the phenotype and the number of damaged cells. The cellular material suitable for the manufacture of CT if he biobazaar contains 80-95% intact cells and more than 80% of cells with the phenotype CD105+CD90+CD45-CD34-. In case of recognition of culture MMSC suitable for the manufacture of CT, from preparing the suspension in the culture medium of DMEM containing 10% fetal bovine serum to cell concentration not less than 1 million/ml For the manufacture of CT use 1 million MMSC 5 cm2EBM.
stage 3. To create a combined transplant DM with MMSC KM.
In terms of the culture unit and ensuring sterility of the transplant DM area of 25-30 cm2placed in a sterile Petri dish, and the appropriate size. In a Petri dish on DM pipette cause cell suspension (MSC) with a concentration of 1 million cells/ml, based on 1 million MMSC 5 cm2DM (for example, 5 ml of suspension containing at least 5-6 million MMSC). For colonization DM MMSC the graft is placed in CO2-incubator at 37°C and 5% concentrations of CO2in the air for 24 hours. Cellular component CT appreciate the vital staining with a fluorescent dye. Preserving sterility CT dropper put sterile fluorescent dye, krasivaya the living cells without damaging them. Using a fluorescent microscope, count the number of adherent cells. CT must contain on its surface 750-1500 th cells on 1 cm2.
Combined transplant is a dermal matrix derived from the donor layer of the dermis by removing cells, fixed to its surface autologous MSC at least 750-1500 thousand on 1 cm2. In appearance CT is a skin plate area of 25-30 cm2thickness of 0.4-0.6 mm In microscopy on the surface of the DM visualized attached cells.
Methods of use.
The method is used in the treatment of wounds requiring plasticity in the deferred period.
Contraindications to the use of the method are:
- blood borne infections
- malignant blood diseases
- reducing the compliance of the patient (dementia and other mental illnesses)
- purulent-septic complications
- fractures of the pelvis.
To calculate required for deferred plastics in the number of CT in PHO, assess the area of the wound. CT is produced in sufficient quantity to complete wound closure.
In the dressing room under sterile conditions in compliance with the rules of asepsis and antisepsis, the patient removes the bandage from the wound. The wound triple handle sterile gauze t is Nanami, soaked in 5% aqueous solution of chlorhexidine, get wet sterile dry gauze pad. To prevent contamination of the wound, the skin around the wound three-treated with 0.5% alcoholic chlorhexidine, avoiding the wound. The wound sterile CT in sterile tray perforined across the surface with a scalpel with a frequency of 1 perforation 5 mm 1 cm2to create ways of outflow of wound, lay on the bottom of the wound cells down model on the wound so that the edges of the CT scan performed at the wound edges is not more than 0.1 to 0.3 see then fixed with a sterile gauze bandage.
Comparative clinical and experimental confirmation of the effectiveness of CT.
In clinical practice we have used DM as a temporary biological coating 7 patients with extensive traumatic defects of the skin. When using DM noted the following positive aspects:
- does not require frequent changes, which avoids daily dressings, subjectively, patients noted decreased pain syndrome;
under the DM creates conducive to healing the microenvironment, the matrix performs a barrier function and prevents contamination of the wound;
- after removing the bottom of the wound covered with bright fine granulations, which prevents the formation of hypertrophic shall ubzow.
But was not observed objective difference in terms of wound healing when using DM and in the treatment of the traditional method.
To confirm the effectiveness of CT was conducted series of experiments on mice.
An experimental study was conducted on 27 outbred white mice. All animals were of the same age (3-5) months. The weight of the specimens was 25-30, Mice were kept on a standard diet of the vivarium. After application of the wound each mouse was kept in a separate cage.
Animals were divided into 3 groups depending on the applied methods of treatment. Anesthetized mice liberated from wool area of the back with scissors was dissected fragment of the skin with a diameter of about 10 mm to fascia. To prevent contraction of the wound edges were added to polyvinyl chloride (PVC) ring with a diameter of 12 mm, Thus, the area of the wound defect was approximately 2% of the body surface.
In group 1 at the bottom of the wound was Packed with sterile gauze swab, moistened with a 5% aqueous solution of chlorhexidine.
In group 2 at the bottom of the wounds were laid DM.
In group 3 at the bottom of the wounds were laid DM attached to its surface autologous MSC (CT).
Headbands to improve contact with the wound was added separate seams, covered with a gauze pad that was attached to the PVC ring.
Conclusion animals from the experiment was performed 3, 7, 14 days. The wound from the surface is scoy was dissected, were evaluated by macroscopic and histological method (degree of maturation of granulation tissue, full epithelialization of the wound surface).
At the conclusion of the experiment in all individuals of a festering wound was not observed. Animals of the first group healing no, the bottom of the wound is covered with granulations. In the second group the bottom of the wound is covered with a scab, tightly fixed on the wound. Animals of the third group showed complete healing of the wound.
Painted hematoxylin-eosin and by the method of van Gison histological specimens were examined at magnification ×100.
In the 1st group using sterile gauze swab, moistened with a 5% aqueous solution of chlorhexidine on 3 days in the bottom of the wound observed necrotic lesions, vascular underlying tissues expanded, full-blooded, with the phenomena of leukostasis and leukodepleted, marginal epithelization poorly expressed. On day 7, on the surface of the wound formed a massive scab, there has been a proliferation of regional epithelial layer with partial ingrowth of scab in the bottom of the wound cell-tissue debris, is formed on the periphery of immature granulation tissue. For 14 days the bottom of the wound filled with immature granulation tissue, epithelialization of the wound surface is incomplete. In granulation tissue, a large number of polim is FNO-nuclear leukocytes (PAL), macrophages and thin-walled vessels.
Thus, to 14 days in this group complete healing of the wound defect is not observed. Remains severe inflammatory infiltration, formed granulation tissue immature. Continues the healing of wounds due to epithelialization.
In group 2 to 3 day DM completely covers the wound and is converted into a homogeneous oxytelinae mass. DM infiltrated by neutrophils at the site of contact with the wound. In the bottom of the wound weak inflammatory infiltration of neutrophils and macrophages, appear isolated fibroblasts, the newly formed vessels are absent. There has been little expansion of the regional epithelial layer with hypertrophy of keratinocytes. On day 7 DM turns into a scab. The bottom of the wound filled with immature granulation tissue with a large number of fibroblasts and moderate amount of PAL and macrophages. There are a large number of newly-formed thin-walled vessels. Small growth boundary epithelial layer of scab. 14 day marked increase in epithelial layer of scab. In granulation tissue remains a large number of thin-walled vessels. There is a slight infiltration of PAL and macrophages, is dominated by fibroblasts.
Thus, the complete healing of the wound defect is not observed. is formed granulation tissue is moderately Mature. Continues the healing of wounds due to severe epithelialization.
In group 3 on 3 day CT completely covers the wound becomes homogeneous oxytelinae mass. CT infiltrated by neutrophils at the site of contact with the wound, it begins to grow regional epithelium at the wound there is a slight inflammatory infiltration, there are newly-formed thin-walled vessels, there is an active migration of fibroblasts. On day 7, significant growth in regenerating epithelial layer under CT, turned into a scab, granulation tissue is moderately Mature with a large number of full-blooded vertically oriented thin-walled vessels of cellular elements is dominated by fibroblasts, there is a moderate amount of leukocytes. 14 day wound covering is missing, has been full epithelialization of the wound surface, the epithelium is only loosely attached to the underlying tissue on its surface begins to form micro, in granulation tissue reduces the number of cellular elements and thin-walled vessels, although they are still vertically oriented, saved a small amount of PAL, the thickness of the formed hem is much less than in the first group.
Thus, when using CT observed acceleration of the maturation of granulation tissue than the Oia with other groups. Inflammatory infiltration of the weak. There is a complete closure of the defect of the newly formed epithelium.
In all groups the healing of the wound defect was due to epithelialization. The best result was observed in the third group, where all mice were epithelialization, which indicates a higher rate of regeneration compared to 1 and 2 groups.
The use of CT allows to stimulate the regeneration of wounds and shortens the period of epithelialization. When using CT were observed reduction phase of inflammation, however, was accelerated maturation of granulation tissue, which indicates the allocation of growth factors, autologous MSC contained in CT. Thus, the reduction phase of inflammation and excretion of growth factors, autologous cells allows to accelerate epithelialization.
The proposed method of obtaining and using CT promising for application in clinical practice, in particular for the recovery of the skin in patients with extensive traumatic wounds with soft tissue defect.
1. Composite graft for repair of the skin, consisting of dermal matrix (DM)obtained from the donor layer of the dermis, with colonized on the surface of autologous mesenchymal multipotent stromal cells is (MMSC), where MMSC contained on the surface of the composite graft in an amount not less 750-1500 th cells on 1 cm2.
2. Composite graft according to claim 1, in which the thickness DM is up to 1 mm.
3. Composite graft according to claim 1, which is designed to treat patients with extensive traumatic soft tissue defects.
4. A method of obtaining a composite graft according to claim 1, including the bone marrow (KM) in patients receiving culture of mesenchymal multipotent stromal cells (MSC), application of a suspension MMSC with cell concentration not less than 1 million/ml on the surface of the dermal matrix (DM) at the rate of 1 million MMSC 5 cm2DM and colonization of the dermal matrix mesenchymal multipotent stromal cells to achieve a number MMSC not less 750-1500 thousand on 1 cm2.
5. The method according to claim 4, in which for getting MMSC of KM on the gradient density Linfoot p=1,077 distinguish nucleated cells, the cells are placed in culture flasks to obtain pesticidesin cells adherent to plastic cells cultured 3-4 passage in the environment DMEM with the addition of 10% fetal bovine serum, culture MMSC, after 3-4 passage assess the suitability of crops MMSC for the manufacture of a combined transplant (KT), and in the case of prirodno and, from preparing the suspension.
6. The method according to claim 5, whereby the cellular material suitable for the manufacture of CT if it contains 80-95% intact cells and more than 80% of cells with the phenotype CD105+CD90+CD45-CD34-.
7. The method according to claim 4, whereby the suspension is prepared in culture medium, DMEM containing 10% fetal bovine serum to cell concentration not less than 1 million/ml
8. The method according to claim 4, in which use dermal matrix (DM)obtained by sampling the skin flap thickness up to 1 mm from the donor-cadaver, separation of the dermis and epidermis, the implementation of the devitalisation of the dermis with getting ready the dermal matrix.
9. Method of recovery of the skin in patients with extensive traumatic wounds with soft tissue defects, including modeling of the shape of the wound composite graft according to claim 1, obtained by the method according to claim 4, after which the combined transplant perforined, lay on the bottom of the wound surface, populated cells down and fix.
10. The method according to claim 8, in which the combined transplant perforined over the entire surface with a frequency of one perforation 5 mm 1 cm2.
11. The method according to claim 8, in which the combined transplant (KT) model on the wound so that the edges of the CT scan performed at the wound edges is not more than 0.1 to 0.3 see
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine and biotechnology. What is presented is a method for improving the immunosuppressive properties of mesenchymal stromal cells of fatty tissues involving human mesenchymal stromal cell culture in DMEM/F12 medium containing penicillin 100 unit/ml and streptomycin 100 unit/ml, glutamine 2 mM, sodium pyruvate 1 mM, 10% foetal bovine serum in the presence of anti-inflammatory TNF-α factor in the concentration of 20 ng/ml and IFN-γ factor in the concentration of 30 ng/ml characterised by the fact that the culture process is carried out in the environment consisting of CO2 5% and air 95% at temperature 37°C for 48 hours.
EFFECT: invention enables improving the immunosuppressive activity of mesenchymal stromal cells and can be used in transplantology and pharmacology.
SUBSTANCE: new strain of hybrid cultured animal cells Mus musculus 2F9 - producer of monoclonal antibodies specific for lysostaphin inhibiting its lytic activity and suitable for its quantitation is proposed. High-affinity monoclonal antibodies (mAb) of hybridoma 2F9 enables to determine lysostaphin in the samples to the concentrations of 0.8 ng/ml. The mAb of hybridoma 2F9 can be used to study the structural and functional properties of lysostaphin and quality control of preparations based on lysostaphin.
EFFECT: improved properties.
3 dwg, 1 tbl, 5 ex
SUBSTANCE: what is presented is a method for cord blood mononuclear cells (cbMNC) expansion ex vivo in the presence of multipotent mesenchymal stromal cells (MMSCs) involving culturing MMSCs from the stromal vascular fraction of fatty tissue to reach a monolayer at the concentration of O2 in the medium of 5%, adding a cbMNC suspension to the MMSCs monolayer, culturing for 72 hours at the concentration of O2 in the medium of 5%, selecting the unattached cbMNCs and replacing the medium, continuing the MMSCs culturing with the attached cbMNCs for 7 days at the concentration of O2 in the medium of 5%.
EFFECT: invention can be used in preparing the stem cell cultures for needs of regeneration medicine.
3 dwg, 3 tbl, 1 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: group of inventions relates to the field of medications and control of their effect. A device for the determination of a chemical compound cardiotoxicity contains a substrate (10), carrying a deformable unit (34), with the unit (34) being partially separated from the substrate by a cavity (32), providing the extraplanar unit deformation. The unit (34) contains the first deformable layer (16), the second deformable layer (20) and a multi-electrode structure (18), layered between the first and second deformable layers. The second deformable layer contains glued to it a cardiomyocyte template (28) and a container for liquid (26). The latter is installed on the substrate for producing an impact on cardiomyocytes with the chemical compound. A method of the claimed device manufacturing includes growth of an oxide layer (12) on the substrate (10), application of the first deformable layer (16) on the oxide layer, application and templating a conductive layer (18) on the first deformable layer with the formation of a multi-electrode structure, application of the second deformable layer (20) on the first deformable layer, templating the second deformable layer to provide access to the multi-electrode structure, application of an adhesive template (24) on the templated second deformable layer, gluing cardiomyocytes (28) to the adhesive template and formation of the cavity (32) under the first deformable layer. The application of the device for purposive search of medications and/or elaboration of medications and to the method of elaboration of a model of a disease with respect to the disease, which is caused or modified with cell stretching, in particular the model of the cardiac disease.
EFFECT: group of inventions ensures an increased accuracy of determination of the chemical compound toxicity with simultaneous simplification of the construction and technology of application.
15 cl, 10 dwg
SUBSTANCE: group of inventions refers to medicine and concerns methods for creation a tooth of a required size, and repair of the lost teeth in the oral cavity by transplantation of the created tooth into the dental loss. Particularly, the method for the tooth creation of a required length in one direction involves the stages: placing the first cell aggregate and the second cell aggregate in a tight contact inside a supporting carrier, wherein the first cell aggregate and the second cell aggregate respectively consists of mesenchymal or epithelial cells; culturing the first and second cell aggregate inside the supporting carrier; the tooth size is corrected by a contact length of the first cell aggregate and the second cell aggregate in the same pre-set direction. A method for determining the contact length of the first and second cell aggregate for the tooth preparation of the required size involves preparing a number of structural types containing structures of various contact length of the first and second cell aggregate in the same pre-set direction; culturing each of a number of structural types inside the supporting carrier; measuring the tooth length prepared at the previous stage in one direction; correlating the given length and contact length providing the basis for calculating the required contact length of the first cell aggregate and the second cell aggregate.
EFFECT: group of inventions enables creating the tooth of the required size that enables preparing a single tooth that can be used as it is in the form of a transplant.
23 cl, 1 tbl, 6 ex, 13 dwg
SUBSTANCE: composition is proposed which comprises stem cells of human amniotic fluid with the phenotype CD73+/CD90+/CD105+/CK19+, nutrient medium, erythropoietin, epidermal growth factor, and collagen taken in an effective amount.
EFFECT: invention enables to increase the proliferative potential and viability of the cells, while simultaneously providing cytoprotective effect on the cells of the transplant and stimulation of migration and proliferation of patient's own cells, and also to reduce significantly the concentration of injectable cells and to activate vascularisation and regeneration at the defect site and can be used in therapy for elimination of congenital and acquired defects of soft tissue arising as the result of injuries, after removal of tumors, congenital diseases, age-related changes or other damages.
2 tbl, 4 ex
SUBSTANCE: invention relates to an agent for treatment of ischemic lesions of tissues, which is a mixture with the ratio of 1.5-3 of two cultures of mesenchymal stem cells, one of which is modified by the genetic structure based on the viral vector which provides hyperproduction of vascular endothelial growth factor, and the other is modified by the genetic structure based on the viral vector which provides hyperproduction of angiopoietin, and a method of treatment of ischemic lesions of tissues by puncture of ischemic tissue, and can be used in medicine.
EFFECT: invention enables to achieve effective vascularisation and repair of ischemic tissue.
4 cl, 4 dwg, 3 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to the field of biotechnology. A method includes stages of cultivating cells, expressing markers, characteristic of a line of pancreatic endocrine cells, in a medium, which contains a sufficient amount of a cyclin-dependent kinase inhibitor to induce increase of MAFA expression. As the cyclin-dependent kinase inhibitor used is ethyl-(6-hydroxy-4-phenylbenzo[4,5]furo[2,3-b])pyridine-carboxylate. The invention can be used in medicine.
EFFECT: claimed is the method of increasing MAFA expression in cells, expressing the markers characteristic of the line of the pancreatic endocrine cells.
3 cl, 10 dwg, 7 tbl, 9 ex
SUBSTANCE: method comprises isolation of mononuclear cells (MNC) from peripheral blood of a patient, separation of cells to adherent and non-adherent fractions, addition of the adherent fraction to the MNC of growth factors, loading of the dendritic cell with antigens of tumour lysate in vitro, the stimulation of maturation of dendritic cells for the next day. At that, the obtained immature DCs are added to lysate-autologous tumour cells at a dose of 100 mcg/ml, and after 48 hours within the subsequent 24 hours the rf-tumour necrosis factor-alpha is applied at a dose of 25 ng/ml. Then, the co-culture is carried out of mature dendritic cells activated with lysate and the non-adherent fraction of MNC at a ratio of 1:10 in the presence of recombinant human interleukin-12 at a dose of 10 ng/ml and the recombinant human interleukin-18 at a dose of 100 ng/ml.
EFFECT: invention enables to improve the level of cytotoxic and interferon-producing activity of antigen-activated dendritic cells while reducing the duration of their culture.
SUBSTANCE: group of inventions refers to medicine, namely to dentistry, and may be used for making a restoration material used for partial dental restoration in the oral cavity. That is ensured by placing the first cell mass formed by mesenchymal or epithelial cells/cell and a second cell mass formed by other mesenchymal or epithelial cells/cell on a carrier. One of the mesenchymal or epithelial cells is made from a dental germ, and the above cell masses are placed in tight contact to each other, but not mixed. The above cell masses are grown to form the whole restored tooth or its germ. That is followed by localising the whole restored tooth or its germ grown that enables implanting the whole restored tooth or is germ within the lost tooth so that a dental crown is directed inside the oral cavity with the dental germ or the tooth being used as the restoration material for preparing an equivalent of the lost tooth within the lost tooth area.
EFFECT: group of inventions enables performing partial dental restoration within the lost tooth area by implanting the restored dental germ or the whole restored tooth, as prepared by the above method.
10 cl, 5 dwg, 2 ex
SUBSTANCE: method for local wound healing involving using a biological dressing which is applied on a wound surface. The biological dressing contains a polymer base of a hydrophobic perforated silicone film coated with a layer of human collagen type I and human diploid cells. The biological dressing is square-shaped and contains the diploid cells in the form of the characterised live cells of M-20 human fibroblasts at the level of passages No. 20-33 in the form of cell monolayers of 70-80% saturation density prepared at a starting density of (4-5)×104 cells per 1 cm2 of the dressing and culturing in a nutrient medium with fibrinolytically active plasma added. Vast injuries may require placing a desired number of the line-on-line dressings on the wound surface. Preferentially, the dressing is applied from the 1st-2nd post-injury day.
EFFECT: improving repair processed in the wound and reducing time of healing.
4 cl, 4 ex, 1 tbl, 3 dwg
SUBSTANCE: medical glue "Neosulphacrylate" contains, wt.p.: ethyl-α-cyanacrylate 76.5-85.0, 3-methacrylcarboxysulpholane 9.0-12.5, decylmetacrylate 6.0-11.0.
EFFECT: glue extends an assortment of compositions of the stated purpose, possesses improved organoleptic properties, increased adhesion and reduced neurotoxicity in tissue gluing, provides an increase of anti-inflammatory action with preservation of such physic-chemical characteristics as rupture strength and elasticity at the level of its prototype.
2 tbl, 6 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to the pharmaceutical industry, namely to an ointment for burns, folliculitis, furunculosis, vasculitis treatment and wound healing. The ointment for burns, folliculitis, furunculosis, vasculitis treatment and wound healing containing: bees wax, line seed oil, kerosene and visceral fat of pig taken in certain proportions.
EFFECT: ointment possesses a biostimulating effect, reduces the wound healing time with no post-therapeutic cicatrisation with good fixation, uniform distribution on the skin surface and ease of use.
9 dwg, 9 ex
SUBSTANCE: experimental wound is cleaned daily, first with normal saline (NaCl), and then coated with modified montmorillonite clay containing 0.1 to 4.35 wt % of silver. The preparation is introduced into the wound in an amount of 0.1 g per a wound of 1.5 cm in diameter.
EFFECT: higher rate of septic wound healing.
3 tbl, 3 dwg
SUBSTANCE: group of inventions relates to medicine, namely to cosmetology, and can be used for treatment of skin aging. For this purpose, used is a medication, which contains a basic fibroblast growth factor (bFGF) as a single active ingredient, which is introduced intracutaneously or subcutaneously into the place of a scar or into the surrounding region, for instance, a keloid, a hypertrophic scar and a scar contracture; in addition, the medication is also intended for treatment of one or more types of skin aging, selected from the following list: skin wrinkles, sagging skin, rough skin, skin thinning and reduction of skin resilience and elasticity because of rupture of dermal tissues or reduction of functions of fibroblast cells, with skin aging being photoaging, and a value of a dose of the basic fibroblast growth factor (bFGF) constituting from 0.1 mcg to 1 mg per 1 cm2 of skin, which represents the treatment target.
EFFECT: inventions ensure significant reduction of wrinkles, improvement of the skin structure, as well as due to an increase of its turgor and an increase of the volume of the subcutaneous adipose cellular tissue.
6 cl, 4 ex, 10 dwg
SUBSTANCE: what is described is a dressing for treating skin conditions and relieving symptoms of skin conditions accompanying blood protein exudation, or for absorbing blood proteins transuding onto the skin.
EFFECT: using the dressing for clearance of waste proteins secreted onto the skin.
8 cl, 22 dwg, 7 ex
SUBSTANCE: ointment contains biologically active substances which are Apis mellifera in an amount of 21-23 wt %, St. John's wort oil in an amount of 12-14 wt %, propolis in an amount of 10-12 wt % and wax in an amount of 7-9 wt %, as well as Vaselin and lanolin as the ointment base.
EFFECT: invention accelerates cell regeneration processes considerably due to a synergetic action of the ingredients.
SUBSTANCE: method involves coating a keratoma surface with a mixture of meadow saffron tincture 1 table spoon and Phellinus tuberculosus tincture 3-4 drops. The mixture is applied once a day on the keratoma and a skin area 1.5-2.0 mm from the keratoma border. The mixture is applied by a thin brush, first with a prime layer and with a second layer after drying.
EFFECT: minimally painful keratoma removal.
SUBSTANCE: material consists of several layers: an inner layer is made from chitosan nanofibres/superfine fibres, and an outside layer are used as an electrical forming substrate and exercise the protective function. The chitosan layer is made from herbal or mixed herbal and animal chitosan and can contain antibiotic. The multilayer material can contain at least one more layer of biopolymer nanofibres/superfine fibres electroformed of cellulose diacetate or gelatin. The three-layer material with the chitosan layer of the nanofibres/superfine fibres is applicable for local wound and burn healing.
EFFECT: material resistance to mechanical stress.
15 cl, 4 dwg, 8 ex
SUBSTANCE: invention refers to medicine, namely dermatology and may be used for stimulating repair of wounds of various geneses. For this purpose, wound cleansing is followed by daily dressings with dihydroquercetin powder applied on a wound surface at the bacterial content no more than 103-4 m.c. per 1 cm2 as a layer of 1-2 mm until wound self-epithelisation, before or after autodermoplasty with a free split-skin flap. That is combined with prescribing the biologically active additive Laviocard+ orally 1 capsule 2 times a day with food for 21 day.
EFFECT: method provides correcting abnormal lipid peroxidation and activating antioxidant protection in local and systemic homeostasis by stimulating and reducing a repair length.
2 ex, 6 tbl
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to the pharmaceutical industry, in particular to the application of the lanocholesterol fraction of wool grease as a bioemulsifier for cosmetic anti-aging preparations. Application of the lanocholesterol fraction of wool grease as the bioemulsifier for cosmetic anti-aging preparations is obtained by alkaline hydrolysis of wool grease with a mixture of ethanol, sodium hydroxide, pyrogallol and water, or with a mixture of ethanol, sodium hydroxide, pyrogallol, toluene and water, taken in a specified ratio, with further separation and purification under certain conditions.
EFFECT: application of the described above fraction from wool grease contributes to long-lasting moistening and enhancement of the water-retaining ability of skin.