Method for local wound healing by means of biological dressing containing live cells of human diploid fibroblasts
SUBSTANCE: method for local wound healing involving using a biological dressing which is applied on a wound surface. The biological dressing contains a polymer base of a hydrophobic perforated silicone film coated with a layer of human collagen type I and human diploid cells. The biological dressing is square-shaped and contains the diploid cells in the form of the characterised live cells of M-20 human fibroblasts at the level of passages No. 20-33 in the form of cell monolayers of 70-80% saturation density prepared at a starting density of (4-5)×104 cells per 1 cm2 of the dressing and culturing in a nutrient medium with fibrinolytically active plasma added. Vast injuries may require placing a desired number of the line-on-line dressings on the wound surface. Preferentially, the dressing is applied from the 1st-2nd post-injury day.
EFFECT: improving repair processed in the wound and reducing time of healing.
4 cl, 4 ex, 1 tbl, 3 dwg
The invention relates to medicine, and biotechnology.
The modern level of development of biology and medicine enables you to create wound dressings containing diploid cells that can actively influence on the process of wound healing [DS. Sarkisov, A.A. Alekseev, V.P. Fogs in kN.: New methods of treatment of burns using cultured alloparental. International Symposium. Saratov, 1998, p.31]. This approach opens the way to improve efficiency, in particular, local edge treatment of burn wounds [S.V. Smirnov, V.B. have been Khvatov. Innovative technologies for the local treatment of burns in the research Institute of emergency care named. N.V. Sklifosovsky. In the book: the New economy. Innovative portrait of Russia. M, the Center for strategic partnership, 2009, s-390].
The prototype. RF patent for the invention №2373944., 23.06.2008 (Method of treatment of burn wounds. Ermolov A.S., Smirnov ST., Grips V.B. have been, Mironov L.L., Conosco I., Zhirkov E.A., Bocharova B.C.). The method of local treatment of burn wounds includes toilet wound overlay on the surface of a biological dressing consisting of hydrophobic perforated silicone film "Carboxyl P"covered
a layer of human collagen type 1, and as allogeneic fibroblasts characterized using cell lines of diploid human fibroblasts M-22 at the level of 15-25 passages, and run what I used with the first two days after burn injury. Local treatment of burn wounds has led to rapid restoration of the epithelium over the entire surface wounds II-IIIA with good functional and cosmetic result. However, used a strain of diploid human fibroblasts M-22 is very demanding and demanding in terms of cultivation, has a lack of proliferative activity that lengthens the time of manufacture of biological dressings and increases the flow cell. The result is the need for cell lines, but is also suitable for the local treatment of pressure sores, bite wounds, long-term healing and burn wounds. For local treatment of skin lesions is proposed to use diploid human cells - fibroblasts line M-20.
Here is a comparative description of the properties of the line M-20 and a licensed line M-22 (used in the prototype) (table).
|Comparative characteristic lines of diploid human cells M-20 and M22|
|Species, cloth||Man, the skin and muscles a 10-week embryo, obtained by performing an abortion on medical until the to be.||Man, the skin and muscles of 8-week embryo, obtained by performing an abortion for medical reasons.|
|Where and when selected||IPWA them. M. Lomakov RAMS, 1986||IPWA them. M.P. Chumakov, RAMS, 1986|
|Characteristics of donor||Cancer, sexually transmitted diseases, hepatitis, tuberculosis in history is not detected; genetic and congenital diseases in the family is not found.|
|The type of growth||fibroblast-like||fibroblast-like|
|Life cycle phase||The formation of 1-3 passages, active growth 4-40, aging from 41 to 48-52.||The formation of 1-3 passages, active growth 4-39, aging 40-70.|
|The method of cultivation||Stationary, inoculation 2 times a week during the active growth phase with a sieving coefficient of 1:2-1:3.|
|Conditions of cultivation||Environment DMEM with the-glutamine with addition of 10% fibrinoliticeski active plasma (FAP) at 37°C and the content of CO 2-5%.||Wednesday Needle MEM with α-glutamine and 10% fetal bovine serum at 37°C and the content of CO2-5%.|
|Separation of cells from the surface growth||A mixture of 0.25% trypsin solution.|
|Conditions of cryopreservation||The composition createsite environment: DMSO 10%, fetal serum of cattle 40%, medium Needle MEM 50%|
|Mode cryopreservation||The starting temperature of 20°C, air temperature drops to +4°C at a rate of 1 deg./min; exposure for 10 minutes, reduce temperature to -30 deg./min with a speed of 1 deg./min; extract 10 minutes; reduce temperature to -150°C at a rate of 10 deg./min. Transfer of frozen samples in liquid nitrogen.|
|Recovery and viability of cells after storage in||In a water bath at 37°C. Viability: not less than 75±5% of cells. Media for cultivation of the same.|
|Karyological properties.||Chariot the p person 2n=46,XY Diploid cells 93,3-to 96.9%. Polyploid cells is not more than 1.6%. The strain has a high genetic stability.||The human karyotype 2n=46,XY Diploid cells 89,7-93,6%). Polyploid cells is not more than 2.3%.|
|Isoenzyme feature||The number of bands isoenzymes MR. FDG and LDH and their electrophoretic mobility coincide with those for human erythrocytes. Mr. FDG slow type|
|Control of contamination:|
|Control of carcinogenic activity|
|In vivo||Not found||Not found|
|In vitro||Not found||Sensitivity to viruses||Poliovirus strains of A. Sabina 1, 2, 3 types a virus vesicularpustular (air force).||The poliovirus strains of A. Sabina 1, 2, 3 types, ECHO, measles, EC, LHM.|
|Biochemical, genetic markers||HLA markers. A*02,03; B*07,40; C*03, 07; DRB1*15, 16; DQB1*05, 06||Not determined.|
|Scope||The test system for determination of interferon status, to obtain a cellular product designed for the treatment of burn (II-IIIa degree) and long-term healing of wounds.||For making polio vaccines from strains of A. Sabina three types.|
In addition, it is shown that the cell line M-20 of 20 arcade produce mRNA α-interferon (Ifnα) and interleukins: IL 1β, 2, 4, 6, 8, 10, 18, that explains the mechanism of improvement of reparative processes in wound-healing.
Passport line of diploid human cells M-20
Designation line: M-20; Species: human; line established in 1986 in the state of IPVA them. M.P. Chumakov RAMS
1. The type of growth: fibroblast-like
2. The number of passages: 50±2
3. Phases of growth: I-1-3 passages, II-4-40, II-41-(50±2)
4. The method of cultivation: stationary, inoculation 2 times a week
5. The cultivation conditions: environment DMEM with α-glutamine with addition of 10% fibrinoliticeski active plasma (FAP) at 37°C and 5% CO2in the atmosphere.
6. Method of separating cells from the surface growth: a mixture of 0.25% solution of trypsin and 0.02% solution of Versene in the ratio (1:1)
Storage conditions: at a temperature of minus 196°C (in liquid nitrogen); (a) composition createsite environment: environment DMEM-50%, cryoprotector DMSO-10%, serum embryos cows - 40%. b) freezing: the starting temperature of 20°C, air temperature drops to +4°C at a rate of 1 deg./min; exposure for 10 minutes, reduce temperature to -30 deg./min with a speed of 1 deg./min; extract 10 minutes; reduce temperature to -150°C at a rate of 10 deg./min. Transfer of frozen samples in liquid nitrogen.
Recovery and viability of cells after cryo-storage:
in a water bath at 37°C. Viability: not less than 75±5% of cells. Media for cultivation of the same.
Isoenzyme description: Number of strips of isoenzymes MR. FDG and LDH and their electrophoretic mobility coincide with those for human erythrocytes. Mr. FDG slow type.
Karyological characteristics: human Karyotype 2n=46, XY. Diploid cells 93,3-to 96.9%. Polyploid cells is not more than 1.6%. The strain has a high gene is political stability.
Control of contamination: bacteria, fungi, mycoplasmas when planting on a selective nutrient medium, the infection of chick embryos and by electron microscopy study revealed that viruses have been detected in animal studies, cell cultures, in chicken embryos, by means of electron microscopy.
Oncogenic security: in vivo - in the study using linear CBA mice tumors were not formed; in vitro control by reverse transcriptase negative.
Biochemical, genetic markers: a) HLA - markers: A*02, 03; B*07, 40; C*03, 07; DRB1*15, 16; DQB1*05, 06. b) Cell line M-20 of 20 arcade produce mRNA α-interferon (Ifnα) and interleukins: IL 1β, 2, 4, 6, 8.10, 18.
Thus, the line of diploid human cells M-20 is characterized according to the methodical recommendations "Certification transplantable cell lines substrates production and control of medical immunobiological preparations RD-42-28-10-89. THE USSR MINISTRY OF HEALTH. M., 1989.- P.16]. It is characterized on the security in accordance with who recommendations and the requirements of Gneissic mibp them. L.A. Tarasevich. In IPVA them. M.P. Chumakov RAMS have seed banks and working cell, capable of providing all the needs of production and research. Line M-20 has a higher proliferative activity than the line M-22, due to the COI is whether under cultivation of cells FAP.
These findings provide the rationale for use of these cells in a new biological dressing to accelerate the healing process in the treatment of bedsores, burns, bite and long-term healing of wounds.
Technical solution aimed at creating a biological dressing with improved properties due to the use of human fibroblasts line M-20, with a higher proliferative activity in the cultivation with the addition of fibrinoliticeski active plasma (FAP), which provides an improvement of reparative processes in the wound and reduce healing time. This armband is made in large cups square shape that provides an additional advantage is the possibility of covering the wound surfaces, including by placing the required number of bands "butt" on the wound field that allows you to close all wound field with extensive damage.
Thus, the object of the invention is a biological dressing for the local treatment of wounds containing polymer base of the hydrophobic perforated silicon film coated with human collagen type I, and diploid human cells, which as a diploid cell contains characterized by living cells fibroblasts line M-20 at the level of p is Saga No. 20-33 in the form of a monolayer of cells to 70-80% of the density of saturation, obtained when the initial density of planting (4-5)×104cells on 1 cm2headbands and cultivation in a nutrient medium with the addition of fibrinoliticeski active plasma (FAP), this armband has a square shape.
The object of the invention is also a method of local treatment of wounds which includes applying to the surface of the wound biological dressings containing polymer base of the hydrophobic perforated silicon film coated with human collagen type I, and diploid human cells, in which a biological dressing is made in the form of "square" and contains as diploid cells characterized by living cells fibroblasts line M-20 level passages No. 20-33 in the form of a monolayer of cells to 70-80% of the density of saturation obtained at initial density of planting (4-5)×104cells on 1 cm2headbands and cultivation in a nutrient medium with the addition of the FAP. When extensive damage can accommodate the required number of bands "butt" on the wound field. Preferably, the bandage should be used 1-2 days after injury.
Example 1. An example of manufacturing a biological dressing.
At the bottom of a square Petri dishes (12×12 cm) with an area of 144 cm2(in the prototype used a round Cup with a diameter of 9 cm and an area of 63 cm2) put hydrophobic, transparent, perforin the bathroom silicone film "Carboxyl-P, cover with a layer of collagen type 1 human, dried, subjected to sterilization by gamma-rays. In a sterile room in the prepared bowl Petri placed a suspension of fibroblasts line M-20 (20-33 passage), seeding of cells is (4-5)×104cells on 1 cm2. Cells cultured overnight in CO2-incubator at 37°C in atmosphere containing 5% CO2. Use a nutrient medium, DMEM with α-glutamine for cell cultures containing 10% FAP. After 18-24 hours, the cells form a monolayer 70-80% density saturation. The thus prepared biological bandage is taken to the clinic in a sealed box (Fig.1, 2).
Figure 1. Biological bandage on the basis of cultivated alloparental line M-20 (and. appearance bandages, century scheme bandages, S. cell line M-20).
Figure 2. Biological dressing containing fibroblast line M-20 before application to the wound.
Example 2. The imposition of a biological dressing in the example of burn wounds: the wound surface to remove dead tissue, products of combustion and pollution. On the prepared wound impose a biological dressing cells (fibroblasts M-20) paying to the surface of the wound. Wound covering fixed wet-drying gauze bandage. The bandage should be used 1-2 days after burn injury. Following ligation are performed every 2-3 days. Transparent PVC the durability of silicone polymer base allows you to monitor the condition of the wound and to monitor its healing, and the gas permeability of the film is comparable to that of healthy skin, provides metabolic comfort in the wound. The hydrophobic nature of the film enables an easy, painless removal in ligation without damage formed on the surface of the wound regenerierung layer neoepitope.
Examples 3-4. Clinical examples of the use of biological dressings containing fibroblasts line M-20 (figures 3 and. - example 3 - example 4):
Example 3. Patient, 32 years old, diagnosis: flame burn I-II-IIIA extent of 65% of the body surface. After 18 hours after injury produced by primary surgical treatment of the wound and the area of 2260 cm2imposed 15 biological dressings containing living fibroblast line M-20 (90 million cells). At 4 days after imposition of a biological dressing (80 million cells across the wound surface of the back layer formed of neoepitopes; 6 days in places applying bandages were observed complete epithelialization of the wound surface without signs of hypertrophy of the epidermis. Brushstrokes-prints from the surface neocapitalist testified to the presence of microflora typical for normal skin. To 12 days in formed epithelium were signs of recovery pigmentation and hair.
Example 4. Patient S., 35 years old, diagnosis: flame burn I-II-IIIA extent of 25% of the body surface. After 24 hours post-injury PR is plagued primary surgical treatment of the wound and the area of 1200 cm 2imposed 8 biological dressings containing living fibroblast line M-20 (40 million cells). On the 4th day imposed a biological dressing containing living fibroblasts (40 million cells)on day 7 in places applying bandages were observed complete epithelialization of the wound surface without signs of hypertrophy of the epidermis. To 15 days in formed epithelium were signs of recovery pigmentation and hair.
Monitoring of patients within the next 2-3 months indicates the formation of a full skin with normal elasticity, pigmentation and hair growth. At check in 3-6 months the skin in the application area of alloparental smooth, supple, normal color with hair. The use of biological dressings comprehensively examined cell lines of live diploid fibroblasts M-20 ensures biological safety cell material and provides improved reparative processes in the burn wound. The presence of seed banks and working cell thoroughly characterized cell lines allows timely and fully meet the needs of the clinic.
1. A biological dressing for the local treatment of wounds containing polymer base of the hydrophobic perforated silicone the film, covered with a layer of human collagen type I, and diploid human cells, characterized in that as a diploid cell contains characterized by living cells fibroblasts line M-20 level passages No. 20-33 in the form of a monolayer of cells to 70-80% of the density of saturation obtained at initial density of planting (4-5)×104cells on 1 cm2headbands and cultivation in a nutrient medium with the addition of fibrinoliticeski active plasma (FAP), this armband has a square shape.
2. The method of local treatment of wounds which includes applying to the surface of the wound biological dressings containing polymer base of the hydrophobic perforated silicon film coated with human collagen type I, and diploid human cells, characterized in that a biological dressing is made in the form of "square" and contains as diploid cells characterized by living cells fibroblasts line M-20 level passages No. 20-33 in the form of a monolayer of cells to 70-80% of the density of saturation obtained at initial density of planting (4-5)×104cells on 1 cm2headbands and cultivation in a nutrient medium with the addition of FAP.
3. The method according to claim 2, characterized in that in case of extensive damage, place the required number of bands "butt" on the wound field.
4. The method according to any the of claim 2 to 3, characterized in that the bandage is applied with 1-2 days after injury.
SUBSTANCE: at the first stage a puncture of the right femoral artery or the right radial artery by Seldinger is performed with further successive catheterisation of the mouth of superior and inferior mesenteric arteries. After that, infusion of 0.5 ml of a 15% solution of xantinol nicotinate and 1.0 ml of a 2% lidocaine solution, diluted in 20.0 ml of a 0.9% solution of sodium chloride is performed. Introduction is carried out under X-ray control.
EFFECT: prevention of endotoxinemia development in the said category of patients due to reduction of spasm of the microcirculatory bed of the small intestine wall, and reduction of oedema and ischemia of the intestine mucosa, blocking the passage of nerve impulses to the intestine wall, which results in reduction of the intestine atony and reduction of translocation of endotoxins from the intestine lumen into the bloodstream.
FIELD: food industry.
SUBSTANCE: what is described is a polymeric composition formulation of poly(hydroxy butyrate-co-hydroxyvalerate) (PHB-co-PHV) with additionally administered poly(D,L-lactide) in a solid ratio of 3:1 and dissolved in chloroform to the concentration of 6-9%; the composition is thereafter mixed for 2 hours and heated to 35°C. What is described is a membrane prepared by electrostatic formation (electrospinning) which involves including biologically active substances of fibronolytic preparations or direct action anticoagulants in the structure of fibres.
EFFECT: membranes possess biocompatible properties, a biodegradation life no more than 60 days, and enable preventing the adhesion formation effectively in experiment.
2 cl, 2 tbl
SUBSTANCE: after a colocolonic anastomosis is created 10-12 cm in a proximal direction from the anastomosis, a double tube is inserted into an intestinal lumen. After a transanal insertion of the tube, an infusion pump is used for dosed introduction of a rifaximin suspension at 0.01 mcg of an active substance per 1 kg of a patient's body weight a minute through one channel and a passive outflow through the second channel. The rifaximin suspension is introduced continuously for the first days, then 3 times a day for 3 hours until observing active intestinal peristalsis.
EFFECT: method provides effective prevention of colocolonic anastomotic leakage, reduces a probability of postoperative complications ensured by avoiding microbial contamination, infection and development of anastomositis, as well as promotes reducing the length of treatment and postoperative stay in hospital.
SUBSTANCE: ronkoleukin is administered in a dose of 500,000 units in a 0.9% physiological saline 20.0 ml. The prepared solution in an amount of 10.0 ml is administered into a round ligament of the liver through a catheter inserted under visual control. The rest amount of 10.0 ml is introduced into incisional wound edges on the 1st and 3rd postoperative days.
EFFECT: method provides stimulating local immunity in both the incisional wound, and the hepatoduodenal region ensured by a separate effect of cytokines on various groups of lymphatic nodes.
SUBSTANCE: invention relates to medicine, namely to surgery and gastroenterology, and can be applied in treatment of patients with acute gastroduodenal bleedings. For this purpose on the 2-nd - 3-rd day after stabilisation of hemostasis a monitor purification with rinsing water is carried out. Temperature of water constitutes 30-32°C, and an intracolonic pressure is 20-25 mm Hg. Adsorbent polyphepan is added into rinsing water in a dose 10 g per 1500 ml of chemically pure decontaminated water of drinking water supply. Speed of supply and exhaust of rinsing water constitutes 1.0-1.6 l/min.
EFFECT: method ensures fast recovery of peristalsis and temperature normalisation, induced by development of endotoxicosis, due to effective purification of intestine from products of blood decay and microbial insemination as a result of selection of optimal parameters of the monitor intestine purification and required adsorbent.
1 tbl, 2 ex
SUBSTANCE: feeding a medical solution is combined with an exposure to low-frequency ultrasound generated by a low-frequency ultrasonic cavitator that is AUZKh-100 PHOTEK apparatus. The medical solution is presented by a solution of metrogyl and dioxydine in ratio 5:1 or 1:1 respectively. The exposure involves feeding the solution at 0.2-0.5 ml/s. The cavitator is placed at 0.2-0.5 cm from a defect surface. First, the defect is exposed to an average-density ultrasonic flow of power 1.6-3 Wt/cm2 for 1-2 min. Then, the exposure is characterised by average-density ultrasonic power 3.1-8 Wt/cm2 for 4-10 min. The therapeutic course is 3-15 sessions every second day.
EFFECT: method provides the maximum mechanical debridement of the purulent-fibrinous impositions and the qualitative wound disinfection ensured by selecting the cavitation mode for specific drug preparations and deep penetration of the above drug preparations into the lesion.
3 cl, 1 ex
SUBSTANCE: invention refers to medicine, namely to therapy, purulent surgery, and may be used for treating chronic wounds. That is ensured by implementing the versions of the methods of treating chronic wounds involving removing a portion of necrotic or other devitalised tissue from the wound with healthy tissue coated with a solvated system of an extracellular polymeric substance. The above system contains an aqueous solution of an agent isolating metal ions, a surfactant and a buffer substance. An osmolarity of the solvated system makes from approximately 1000 to approximately 4000 mOsm of the dissolved substance per one litre. What is also presented is a device for treating the given pathology comprising a wound cleansing instrument, a container for the solvated system of the extracellular polymeric substance, and an aspirator. What is presented is a kit for care for the patients suffering chronic wounds, comprising a tray, a syringe, a vessel with the solvated system of the extracellular polymeric substance, and printed application instructions.
EFFECT: presented group of inventions provide the effective treatment of chronic wounds by introducing the solvated system of the extracellular polymeric substance biocompatible with healthy tissues containing the ingredients preventing the binding of polymer chains in the extracellular polymeric material of a biofilm matrix and promoting transferring them into the solution for more complete removal from the wound.
67 cl, 2 tbl, 1 ex, 3 dwg
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine, particularly to traumatology, orthopaedis, maxillofacial surgery, dentistry, neurosurgery, namely to osteoplastic formulation of biocomposites for treating human bone diseases and injures, and can be used as a material fully degradable in the body and replaceable by new bone tissue, for bone cell regeneration, as a biological filler for bone tissue defects, as an osteoconductive and osteoinductive biological frame for bone tissue regeneration. The hardened biocomposite for bone defect replacement on the basis of calcium phosphate contains magnesium phosphate, calcium hydrophosphate, amino acid; calcium phosphate is biological hydroxyapatite in the following proportions, wt %: hydroxyapatite - 45-47, magnesium phosphate - 32-34, calcium hydrophosphate - 16-19, amino acid - 1-5, as a binding agent - casein.
EFFECT: implementing the expansion of the biocomposites for bone defect replacement and endoprosthesis fixation.
SUBSTANCE: invention refers to medicine, particularly coloproctology, and may be used for treating chronic anal fissure. The method involves an anal fissurectomy with a graduated sphincterotomy and a drug-induced therapy. A patient's preoperative psychoemotional status is assessed by questionnaire survey according to the Hospital Anxiety and Depression Scale (HADS). If the disorders have been shown by the questionnaire survey, namely 8-10 points "subclinical anxiety/depression" or 11 points and more "clinical anxiety/depression", a psychopharmaceutical therapy with Grandaxin or Tianeptine. The preparations are administered in common doses for 4 weeks.
EFFECT: method provides the effective treatment of the disease with comorbid psychoemotional disorders, exposure on the additional mechanisms of the anal fissure pathogenesis in this case.
SUBSTANCE: invention refers to medicine, more specifically to injectable bioresorbable compositions of biocomposite materials for treating human bone diseases and disorders as: a material able to be biodegradable in the body completely and to be replaced by new bone tissue for the purpose of bone cell regeneration, osteoconductive and osteoinductive biological support frame for bone tissue regeneration, applied in traumatology, orthopaedics, maxillofacial surgery, and neurosurgery. The injectable biocomposite material contains biological hydroxyapatite, calcium hydrophosphate, amino acid arginine, as well as phosphoprotein (casein) recovered from skim milk, and glutaric aldehyde as a hardener.
EFFECT: preparing the bioresorbable material having the biocompatibility and the pronounced osteoconductive and osteoinductive properties.
2 cl, 1 ex
SUBSTANCE: medical glue "Neosulphacrylate" contains, wt.p.: ethyl-α-cyanacrylate 76.5-85.0, 3-methacrylcarboxysulpholane 9.0-12.5, decylmetacrylate 6.0-11.0.
EFFECT: glue extends an assortment of compositions of the stated purpose, possesses improved organoleptic properties, increased adhesion and reduced neurotoxicity in tissue gluing, provides an increase of anti-inflammatory action with preservation of such physic-chemical characteristics as rupture strength and elasticity at the level of its prototype.
2 tbl, 6 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to the pharmaceutical industry, namely to an ointment for burns, folliculitis, furunculosis, vasculitis treatment and wound healing. The ointment for burns, folliculitis, furunculosis, vasculitis treatment and wound healing containing: bees wax, line seed oil, kerosene and visceral fat of pig taken in certain proportions.
EFFECT: ointment possesses a biostimulating effect, reduces the wound healing time with no post-therapeutic cicatrisation with good fixation, uniform distribution on the skin surface and ease of use.
9 dwg, 9 ex
SUBSTANCE: experimental wound is cleaned daily, first with normal saline (NaCl), and then coated with modified montmorillonite clay containing 0.1 to 4.35 wt % of silver. The preparation is introduced into the wound in an amount of 0.1 g per a wound of 1.5 cm in diameter.
EFFECT: higher rate of septic wound healing.
3 tbl, 3 dwg
SUBSTANCE: group of inventions relates to medicine, namely to cosmetology, and can be used for treatment of skin aging. For this purpose, used is a medication, which contains a basic fibroblast growth factor (bFGF) as a single active ingredient, which is introduced intracutaneously or subcutaneously into the place of a scar or into the surrounding region, for instance, a keloid, a hypertrophic scar and a scar contracture; in addition, the medication is also intended for treatment of one or more types of skin aging, selected from the following list: skin wrinkles, sagging skin, rough skin, skin thinning and reduction of skin resilience and elasticity because of rupture of dermal tissues or reduction of functions of fibroblast cells, with skin aging being photoaging, and a value of a dose of the basic fibroblast growth factor (bFGF) constituting from 0.1 mcg to 1 mg per 1 cm2 of skin, which represents the treatment target.
EFFECT: inventions ensure significant reduction of wrinkles, improvement of the skin structure, as well as due to an increase of its turgor and an increase of the volume of the subcutaneous adipose cellular tissue.
6 cl, 4 ex, 10 dwg
SUBSTANCE: what is described is a dressing for treating skin conditions and relieving symptoms of skin conditions accompanying blood protein exudation, or for absorbing blood proteins transuding onto the skin.
EFFECT: using the dressing for clearance of waste proteins secreted onto the skin.
8 cl, 22 dwg, 7 ex
SUBSTANCE: ointment contains biologically active substances which are Apis mellifera in an amount of 21-23 wt %, St. John's wort oil in an amount of 12-14 wt %, propolis in an amount of 10-12 wt % and wax in an amount of 7-9 wt %, as well as Vaselin and lanolin as the ointment base.
EFFECT: invention accelerates cell regeneration processes considerably due to a synergetic action of the ingredients.
SUBSTANCE: method involves coating a keratoma surface with a mixture of meadow saffron tincture 1 table spoon and Phellinus tuberculosus tincture 3-4 drops. The mixture is applied once a day on the keratoma and a skin area 1.5-2.0 mm from the keratoma border. The mixture is applied by a thin brush, first with a prime layer and with a second layer after drying.
EFFECT: minimally painful keratoma removal.
SUBSTANCE: material consists of several layers: an inner layer is made from chitosan nanofibres/superfine fibres, and an outside layer are used as an electrical forming substrate and exercise the protective function. The chitosan layer is made from herbal or mixed herbal and animal chitosan and can contain antibiotic. The multilayer material can contain at least one more layer of biopolymer nanofibres/superfine fibres electroformed of cellulose diacetate or gelatin. The three-layer material with the chitosan layer of the nanofibres/superfine fibres is applicable for local wound and burn healing.
EFFECT: material resistance to mechanical stress.
15 cl, 4 dwg, 8 ex
SUBSTANCE: invention refers to medicine, namely dermatology and may be used for stimulating repair of wounds of various geneses. For this purpose, wound cleansing is followed by daily dressings with dihydroquercetin powder applied on a wound surface at the bacterial content no more than 103-4 m.c. per 1 cm2 as a layer of 1-2 mm until wound self-epithelisation, before or after autodermoplasty with a free split-skin flap. That is combined with prescribing the biologically active additive Laviocard+ orally 1 capsule 2 times a day with food for 21 day.
EFFECT: method provides correcting abnormal lipid peroxidation and activating antioxidant protection in local and systemic homeostasis by stimulating and reducing a repair length.
2 ex, 6 tbl
SUBSTANCE: invention can be used for treating degree III-IV radiation injuries on radiation fields following neutron therapy in patients suffering from local recurrent breast cancer (BC). That is ensured by wound irrigation with ozonised distilled water with ozone concentration of 10,000 mcg/l; a skin ulcerous defect is processed in a vacuum container for 10-15 minutes with gaseous ozone with the concentration of 10,000 mcg/l. That is followed by ozonised air applications with the parameters of: peroxide number - 18%, acid number - 71 mg CFU/g on the skin ulcerous defect once or twice a day for 10-15 days every 5-7 days for 2-3 courses.
EFFECT: ulcer detersion from purulent deposits, active regeneration and the skin ulcerous defect healing.
6 dwg, 2 ex
FIELD: textiles, paper.
SUBSTANCE: invention can be used in the textile industry for production of antimicrobial argentiferous cellulosic materials. The method of production of antimicrobial argentiferous cellulosic material comprises processing of the cellulose matrix by the aqueous dispersion of the silver particles for 10-60 sec, obtained by mixing an alkali extract of bast fibres with an aqueous solution of silver nitrate and keeping this mixture at a temperature of 50-95°C for 10-90 min.
EFFECT: invention enables to reduce loss of the active components, to reduce the time and energy consumption during production of antimicrobial materials and also to improve the quality of the processed nonwoven material and environmental safety.
4 cl, 2 tbl, 8 ex