Protection of bioanalytical chambers for samples
SUBSTANCE: invention relates to a cartridge for a bioanalytical reaction device. The cartridge contains at least one chamber for a sample, which has a wall, through which the said sample can be processed or analysed by the bioanalytical reaction device. The cartridge also contains a case and a platform, and the platform contains the said at least one chamber for the sample and is connected with a possibility of travel with the case in such a way that the platform is able to move between the removed position, in which the wall is protected by the case, and the protruded position, in which the wall is outside the case, by putting operating means in action. The bioanalytical reaction device has a slot for reception of the cartridge, and contains an operating device for moving out and removal of the platform.
EFFECT: technical result which is achieved lies in provision of protection of the sample from contamination and damage without excessive complication of the cartridge construction.
15 cl, 5 dwg
The technical field
This invention relates to a device for performing bioanalytical processing and analysis. In particular the present invention relates to a bioanalytical reaction device and cartridge. This cartridge contains at least one camera for samples for storage of biological samples, which bioanalytical reaction device can process and analyze.
The level of technology
One example of a bioanalytical reaction is a DNA-polymerase chain reaction. Polymerase chain reaction (PCR) is a technology that provides amplification and detection sequences polynucleotides. This technology has a wide range of applications, including the analysis of DNA sequences, detection of genetic mutations, the diagnosis of viral infections and many others. Using PCR specific sequence targets or strand of DNA can be exponentially amplified. Polymerase chain reaction contains cycles of denaturation targets when heated samples, primer, origaudio at a lower temperature, and mediated polymerase pulling at slightly higher temperature. In the last step the DNA polymerase synthesizes a new DNA strand in addition to the matrix circuit Dnpi optimal conditions, the number of strands of the target DNA is duplicated.
In addition to PCR-known other bioanalytical reactions such as ligase chain reaction. In General, some international bioanalytical methods depend on the temperature change of the sample orderly manner. In this regard, there is a need to automate these methods.
The level technique known some mechanical and automated bioanalytical reaction device. Some devices use the cartridge for storage of biological samples so that one cartridge can temporarily stored one or more biological samples, while a biological sample in another cartridge can be processed in the bioanalytical reaction device. The operator need only remove one cartridge and insert another cartridge into the device.
Such cartridges have different interfaces, such as one or more interfaces for heating the sample in the cartridge, as well as one or more interfaces for the optical readout of the reaction, which, for example, is indicated by a specific color samples or covering certain substances.
More specifically, the processed samples are stored in one or more chambers in the cartridge. In General, the interface is provided by a wall of a chamber through which the sample can be heated and analyzed. If the should be performed optical reading, the camera needs to translucent wall interface.
A problem may arise in that such interfaces can be damaged or become dirty. In particular, when the operator takes the hands of such a cartridge, there is a possibility that it touches the cartridge at the location of the interface. The interface in the form of a thin wall may already be damaged by the force applied by the finger. Besides thus can settle on the interface sweat or fat. Damaged or contaminated interface may leak from the cartridge or distortion of optical detection.
The objective of the invention is the provision of nepodrazhaemogo and clean cartridge.
In an exemplary variant of execution of the invention is provided a cartridge for bioanalytical reaction device, and the cartridge contains at least one camera for the sample, and at least one camera for the sample has a wall through which the sample can be processed or analyzed bioanalytical reaction device in which the cartridge includes a housing and platform, and the platform includes at least one camera for the sample, while the platform is connected to the housing for movement so that the platform is moved between the retracted Polo is the group of in which the wall is protected by a housing, and an extended position in which the wall is located outside the housing.
This cartridge is protected from the risk of damage or contamination without undue complication of the design of the cartridge and bioanalytical reaction device.
It is clear that here the term "cartridge" is used for each type of device that can connect to the bioanalytical reaction device. For example, the cartridge can be a holder, shop, cartridge or substrate.
At least one camera for the sample placed on the platform (or disk, or substrate), which may be ejected from the cartridge. In the retracted position the camera for the sample is inside the cartridge. Therefore, the camera is protected from damage or contamination. To use the platform extends from the cartridge, for example to access the interface with heaters and optical sensors biological reaction device.
The wall of the at least one camera for a sample may be heating the interface or, if the wall is translucent (at least for a certain wavelength), the optical interface to interact with components of the bioanalytical reaction device, such as a heater or an optical sensor.
Additional PR is dimensional variant execution is provided a cartridge, in which at least one camera for the sample is connected to the channel for filling at least one chamber for the sample, and this channel ends near Executive tools.
"Near" can be understood as referring to the length of one of the following intervals: [0, 15 mm], [0, 10 mm], [0, 5 mm].
At least one camera for the sample is connected to the channel for filling and draining at least one camera for the sample fluid, such as the solution in which the dissolved sample. Instead of the channel can be used every means, configured to conduct the fluid from one point to another, as, for example, tubing, pipe, or sleeve. One end of the channel may be connected to the pipeline bioanalytical reaction device that can pump the fluid through the pipeline in the camera for testing. This end of the channel is a part of the fluid interface of the cartridge.
The location of this end of the channel near the Executive means has the advantage that the mechanical connection to move the platform and the connection fluid can be combined in a single component cartridge.
Additional exemplary variant execution is provided a cartridge, in which a part of the channel located inside the Executive tools. The channel can be rasmusen shaft for rotation of the platform or in the spindle to move the platform. This gives one the possibility of combining mechanical and connections for fluid cartridge. Additionally, at least one camera for a sample may be filled regardless of the position of the platform.
Additional exemplary variant execution is provided a cartridge, in which the wall is located on the front side of the platform, and the platform has a second side opposite the first side, and with the platform in its extended position accessible from the first side and the second side of the bioanalytical reaction device for processing or analysis of the sample. Sample within at least the camera for a sample may be processed or analyzed equally on both sides of the platform.
According to additional exemplary variant execution is provided a cartridge in which at least one size of cartridge with the platform in its extended position is greater than this size cartridge platform in the retracted position. In this regard, the cartridge with the platform in the retracted position can easily be stored.
According to additional exemplary variant execution is provided a cartridge, in which the platform is connected rotatably to the body. Preferably, the actuating means is a shaft, the platform is connected with the shaft for rotation of the platform around the axis of rotation. More preferably, the shaft continues until the holes in the housing. Thus, it can be easily installed mechanical connection of the contractor bioanalytical reaction device with the cartridge for rotation of the platform. Additionally, the hole in the body can provide a guide for the shaft, and means for the platform.
Alternative additional exemplary variant execution is provided a cartridge, in which the platform is connected with the slidable housing. The actuating means may be a spindle for translational movement of the platform from a retracted position into the extended position.
According to additional exemplary variant of execution, provided a cartridge, in which the platform has the form of a plate, which in the retracted position located between the first wall and the second wall of the housing. Platform in the form of plates, i.e. the component with one dimension much smaller the other two dimensions in different directions, may be provided with more than one camera for samples with camera samples readily available bioanalytical reaction device.
According to additional exemplary variant execution is provided a cartridge, in which the wall of the at least one camera for thin samples. To reduce thermal barrier wall can be tone is Oh and maybe for example, be a foil of high thermal conductivity. Here about the thin walls, I mean a wall that has a thickness of less than about 200 microns. The thin wall can also optimize bandwidth optical interface at least one camera for testing.
According to additional exemplary variant execution is provided a cartridge in which at least one camera for the sample formed by the hole in the platform, which is covered with a foil or a thin layer, forming a thin wall.
Another object of the invention is the bioanalytical reaction device having a slit or a receiver for receiving a cartridge containing the contractor for extension and retraction of the platform of the cartridge. The contractor may be a stepper motor.
According to additional exemplary variant of execution provided bioanalytical reaction device having a capacity for filling at least one chamber for the sample, and the container is connected to at least one camera for the sample pipeline ending in the mechanical connection between the contractor and the Executive the means to move the platform. Inside the mechanical connection may also be a connection for fluid bioanalytical reaction device with the cartridge. Fluid is the tuner or the connection fluid bioanalytical reaction device and the mechanical connection are combined in one component.
According to additional exemplary variant of execution provided bioanalytical reaction device having a presence sensor cartridge to detect the presence and/or correct insertion of the cartridge into the slot. Only when a cartridge is present in the gap, bioanalytical reaction device will affect the pipeline to fill the chamber for the sample. Otherwise, fluids can contaminate the interior bioanalytical reaction device.
According to additional exemplary variant of execution provided bioanalytical reaction device, which is performed with the opportunity to put into action the contractor to move the platform in the extended position when the presence sensor cartridge detects the presence of the cartridge in the slot.
These and other aspects of the invention will be apparent from the following description and explained by reference to the following variant of execution.
Brief description of drawings
Below an embodiment of the present invention is described in more detail with reference to the attached drawings
Figure 1 shows a perspective view of a cartridge for bioanalytical reaction device with the platform in the retracted position.
Figure 2 shows a perspective view of the cartridge of figure 1 with the platform in its extended put the I.
Figure 3 shows a schematic view in cross section of the platform in figure 2.
Figure 4 is a schematic top view of the platform in figure 2.
Figure 5 shows a schematic representation of functional components of the bioanalytical reaction device.
A similar description of options
Figure 1 shows a perspective view of the cartridge 10 for bioanalytical reaction device. The cartridge 10 has a housing 12 with a top cover or wall 14 and the bottom cover or wall 16. It is clear that the terms "upper" and "lower" are used to simplify and not intended to be limiting. For example, the cartridge 10 can be inserted into the bioanalytical reaction device not shown in the direction and in the vertical direction.
Figure 1 shows the platform 30 in the retracted position. The platform 30 is connected rotatably with the housing 12 by means of shaft 32 as the actuator. The shaft 32 is directed by the hole 33 in the top cover 14. Rotation of the shaft 32 around the axis of rotation of the platform 30 may be ejected from the housing 12 of the cartridge 10.
Figure 2 shows a perspective view of the cartridge 10 with the platform 30 in its extended position. The platform 30 is released from the housing 12 through a slot 18 in the body 12 between the top cap 14 and bottom cap 16. Additional rotation of the shaft 32 in isobologram direction around the axis of rotation of the platform 30 may be again retracted in the casing 12. In the retracted position, the platform 30 is protected from damage or contamination. In the retracted position, the platform 30 may be made available to performers, such as the heater and the sensor bioanalytical reaction device.
Additionally, figure 2 can be seen that the platform 30 contains five chambers 34 for samples.
Figure 3 shows a schematic view in cross section of the platform 30. In particular, the left side of the drawing shows a view in cross section of the chamber 34 to sample the right side of the drawing shows a view in cross-section surrounding the axis And rotation.
The platform 30 includes a plate 38, which may be made of plastic. For each camera 34 sample has a hole 36 in the plate 38. One of the first side plate 38 is superimposed on the first or top foil 40. For example, the top foil 40 may be glued to the plate 38. In the shown embodiment, the upper foil 40 has a thickness of about 100 μm. In this area of the holes 36 of the upper foil 40 forms a thin wall of the chamber for the sample, and this thin wall represents heating the interface 44 of the chamber 34 for the sample. If the source of heating or cooling are placed outside the chamber 34 to sample in the field of heating interface 44, heat can be transferred in the internal region of the chamber 34 for samples or leave it.
the and the other second side plate 38, opposite the first side, overlaps with the second or bottom foil 42 translucent material. The bottom foil 42 may be glued or in some other way connected to the plate 38. Moreover, the bottom foil 42 has a thickness of about 100 μm. In the area of the hole 34 of the lower foil 42 forms an optical interface 46 of the chamber 34 for the sample. In this field light can penetrate the translucent bottom foil 42. The light coming from the inner region of the chamber for the sample, can be detected by the optical sensor placed near the optical interface 46 of the chamber 34 for a sample.
Additionally, figure 3 shows the first channel 48 formed by the groove or recess in the surface of the plate 38 and covered the top with foil 40. In the same way, the second channel 50 formed by connecting the camera 34 for the sample with the third channel 52 on the inside of the shaft 32.
It is clear that there are other opportunities for education of the camera 34 to sample and channels 48, 50, 52 within the platform 30. For example, the platform 30 may be made of two parts that are mirror-symmetric with holes and grooves, which form a chamber for the sample and channels, when these two parts are connected with each other. Additionally, it is possible to provide the plate 30 nests. Camera sample can be formed on the plate with foil or a thin layer covering these jacks. This is the case of these cameras for the sample will have only one interface.
Figure 4, represents a schematic top view of the platform 30 can be seen that the chamber 34 for samples connected by fluid through channels 48, 50 with channels 52 formed in the shaft 32 near the axis of rotation. The channels 48 and 50 each chamber 34 for a sample may be filled with fluids, for example a solution containing the DNA fragments to be analyzed or amplification. In addition, the camera 34 for samples can be made conductive gas, such as air, or other fluids or liquids, for example water, through the channels 48, 50 into the chamber 34 for a sample.
The shaft 32 with the channel 52 is a fluid interface 54 of the platform 30.
As fluid interface 54 is located near the axis of rotation, it can be available within the mechanical connection bioanalytical reaction device for rotation of the platform 30. Therefore, the mechanical connection and the connection for fluid of the joint, and the number of connections between the cartridge 10 and bioanalytical reaction device is reduced.
Figure 5 shows a schematic illustration of the bioanalytical reaction device 60. Bioanalytical reaction device 60 has a slot 62 for receiving the cartridge 10. With the help of a contractor 64, for example a stepper motor, which is connected for rotation with the shaft 32, the platform 30 may be nominated from install the ja 10 in the extended position and to return in the retracted position. Figure 5 shows the platform 30 in its extended position. Pipelines 70 for fluid connected to the inlets and outlets, combined with a mechanical connection 66. Inlets and releases are installed in their respective mating parts formed in the shaft 32. Pumping and displacing mechanism 68 may fill the chamber 34 to the sample in the platform 30. Bioanalytical reaction device has one or more heaters 72 for heating the sample inside the chambers 34 for samples with the first side of the platform 30 and one or more optical sensors 74 for analysis of the light emitted from the inner region of the chambers 34 for samples from the second side of the platform 30.
Using the controller 76, which is connected through pipes 78 management contractor 64, the inlet and the displacing mechanism 68, the heater 72 and the optical sensor 74, bioanalytical reaction device 60 can control the analysis and processing of samples in the cells for sample automatic way. For example, bioanalytical reaction device 60 may perform the above-mentioned PCR procedure.
Additionally, it is possible that the bioanalytical reaction device 60 controls the extension and the service platform 30 automatic way. When the operator inserts the cartridge 10 into the slot 62, the mechanical sensor 80 detects the presence of the cartridge 10. Al is ernative, detection can be performed using an optical sensor. With the introduction of the regulator 76 instructs the performer 64 to rotate the platform 30 in the extended position. Then, the controller 76 can be performed some processing, such as filling cameras of different solutions, the heating chambers 34 to sample and analyze the light coming from the camera 34 to sample. When processing and analysis performed, the controller 76 instructs the performer 64 to rotate the platform 30 back in the retracted position, the operator can remove the cartridge 10 from the bioanalytical reaction device 60.
Although the invention has been illustrated and described in detail in the drawings and foregoing description, such illustration and description should be considered illustrative or about and not limiting the invention which is not limited to the disclosed variants of execution. Any changes in the disclosed embodiment can be understood and carried out by a specialist in the field of machinery, while in practice the claimed invention on the basis of a study of the drawings, description and appended claims. In the claims the expression "comprising" does not exclude other elements or steps. A single processor or controller or other unit may fulfill the functions of several items listed in FD is the mule of the invention. The fact that certain measurements are listed in vzaimootnoshyeniya dependent claims does not indicate that the Association of these measurements cannot be used for good. Any reference positions in the claims should not be construed as limiting the scope of invention.
1. The cartridge (10) for bioanalytical reaction device (60)and the cartridge (10) comprises at least one chamber (34) for the sample, and the specified at least one chamber (34) for the sample has a wall through which the sample can be processed or analyzed bioanalytical reaction device (60),
the cartridge (10) includes a housing and a platform (30), with the platform (30) contains the specified at least one chamber (34) for samples
the platform (30) is connected to move with the body so that the actuation of the Executive tools platform (30) is able to move between the retracted position in which the wall is protected by a housing, and an extended position in which the wall is located outside the housing.
2. The cartridge (10) according to claim 1, in which indicated at least one chamber (34) for the sample is connected to the channel to fill the specified at least one chamber (34) for the sample, and the channel ends near the Executive among the STV.
3. The cartridge (10) according to claim 1, in which indicated at least one chamber (34) for the sample is connected to the channel to fill the specified at least one chamber (34) for the sample, and the part of the channel located inside the Executive tools.
4. The cartridge (10) according to claim 1, in which the wall is positioned on the first side of the platform (30), with the platform (30) has a second side opposite the first side, and with the platform in its extended position accessible from the first side and the second side of the bioanalytical reaction device (60) for processing or analysis of the sample.
5. The cartridge (10) according to claim 1, in which at least one size of the cartridge (10) with the platform (30) is in its extended position is greater than this size cartridge (10) with the platform (30) in the retracted position.
6. The cartridge (10) according to claim 1, in which the platform is connected to the housing for rotation.
7. The cartridge (10) according to claim 6, in which the actuating means is a shaft, the platform is connected with the shaft for rotation of the platform (30) around the rotation axis.
8. The cartridge (10) according to claim 1, in which the platform is connected to the housing slidable.
9. The cartridge (10) according to claim 1, in which the platform has the form of a plate, which in the retracted position located between the first wall and the second wall of the housing.
10. The cartridge (10) according to claim 1, in which indicated at least about the on camera (34) for the sample formed by the hole in the platform (30), which is covered with foil, forming a wall.
11. Bioanalytical reaction device (60)having a slot for receiving the cartridge (10) according to claim 1, containing the contractor for extension and lowering of the platform (30) of the cartridge (10).
12. Bioanalytical reaction device (60) according to claim 11, in which the executor is the engine.
13. Bioanalytical reaction device (60) according to item 12, which has the capacity to fill the specified at least one chamber (34) for the sample, and the capacitance connected to the specified at least one camera (34) for the sample pipeline ending in the mechanical connection between the contractor and the Executive means for moving the platform (30).
14. Bioanalytical reaction device (60) according to claim 11, having a presence sensor cartridge to detect the presence of the cartridge (10) in the gap.
15. Bioanalytical reaction device (60) 14, which is performed with the opportunity to put into action the contractor to move the platform (30) into the extended position when the presence sensor cartridge detects the presence of the cartridge (10) in the gap.
SUBSTANCE: method involves the morphological examination of peripheral blood according to Lythos system technology. A blood sample is divided into two portions; one of them is kept for 2-2.5 hours at temperature +37°C. Preparing both samples according to the above technique is followed by a comparative examination to form concretions in sample fascias and counting the number of lines. If the number of lines of the formed concretions in the thermally treated sample fascia is related to the number of lines of the formed concretions in the other sample as three or more, the body adaptation is considered to be high; if the relation falls within the range of one to three, the body adaptation is moderate, while the relation being of zero to one show the low body adaptation. The invention can be applicable to state individual workability in occupational hazards, sub-extreme and extreme environment; for sportsmen; determining the indications for blood donation with no health damage; predicting the clinical course and outcome of an expected surgery, as well as controlling the effectiveness of the prescribed therapeutic and preventive actions.
EFFECT: method enables more accurate evaluation characteristics in the earlier period by detecting markers determining the energetic adequacy of protein structures.
4 dwg, 3 ex
SUBSTANCE: initial blood plasma glucose is measured. That is followed by infusion of a glucose-insulin mixture in the ratio of 1 unit of insulin to 4 g of glucose at 2 mg/kg/min of glucose. Blood plasma glucose is measured after the infusion of the glucose-insulin mixture, and if observing a blood plasma glucose gain by more than 0.7 mmole/l, insulin-resistance is diagnosed.
EFFECT: method provides the effective and easy to implement diagnosis of insulin-resistance which may be used in outpatient practice.
1 dwg, 1 tbl, 1 ex
SUBSTANCE: invention refers to a method for prediction of increasing a cardio-malleolar-vascular stiffness index in the patients with chronic obstructive pulmonary disease combined with ischemic heart disease which involves studying initial systemic inflammatory biomarkers of C-reactive protein (CRP), tumour necrosis factor-alpha (TNF-alpha) and anti-inflammatory interleukine 4 (IL-4) and calculating the discriminant equation D=1.42*(TNF-α)+0.78*(CRP)-0.534*(IL-4), and if D is more than 4.82, a one-year increase of the cardio-malleolar-vascular stiffness index for one year is predicted, and if D is 4.82 or less, no increase of the cardio-malleolar-vascular stiffness index is predicted.
EFFECT: more effective prediction of increasing the cardio-malleolar-vascular stiffness index.
SUBSTANCE: invention refers to medicine, and can be used for the prediction of developing bacterial infections in the patients suffering acute respiratory viral infection prior to clinical presentations. Substance of the method: NAPD-dependent glutamate dehydrogenase activity in peripheral blood lymphocytes of the patient suffering acute viral infection is measured. If the value is more than 2.89 mcU/10000 cells, the bacterial complications are predicted to develop within 3-5 days after admission to hospital.
EFFECT: method implying measuring the one value enables predicting the developing bacterial infections prior to their clinical presentations in the patients suffering an acute respiratory viral disease with 92,31% confidence, conducting the early treatment with antiviral preparations and metabolic immune protectors to prevent the developing bacterial complications.
1 tbl, 1 ex
SUBSTANCE: group of inventions refers to medical instrument engineering. Skin and a calibration sample are exposed to optical radiation in at least Nλ≥3 narrow or wide spectral regions Λk (k=1,…,N). Signals emitted by the skin and calibration sample are recorded with an activated and de-activated radiation emitter. Diffusion reflection factors R(Λk) are derived from the relation
EFFECT: group of inventions enables providing more accurate determination of the depth penetration of light into the skin by eliminating the use of a priori information of an object being tested, the effect of a spread of instrumental constants of the reflected signal recording system, eliminating a contribution of the emission reflected from the skin surface into the recorded optical signals.
2 cl, 11 dwg
SUBSTANCE: group of inventions relates to versions of a method and a device for performing a sample analysis for different analytes. The method includes contact of a body of separated zones of an analysis with a liquid sample, for instance, with whole blood. The zones of analysis are located inside a channel of a microfluidic device. The channel is formed by at least one flexible wall and the second wall, which can be flexible or not. Each zone of the analysis contains a probe-compound, specific to the respective target analyte. The microfluidic device is compressed to reduce the channel thickness, which represents a distance between internal surfaces of walls inside the channel. The presence of each analyte is determined by means of optic detection of interaction in each of a multitude of the analysis zones, for which the distance between internal surfaces in the respective location is reduced. Interaction in each analysis zone indicates the presence of the target analyte in the sample. Capillary structures of devices or those used in methods can contain a matrix, the devices can contain control elements, and, therefore, the methods of carrying out the sample analysis can apply respective control functions.
EFFECT: providing more accurate qualitative and/or quantitative determination of analytes in the sample.
154 cl, 38 dwg
SUBSTANCE: blood serum neopterin is measured in adolescent girls suffering FROM normogonadotropic hypoovarianism and unspecified oligomenorrhea by immunofluorescent assay. If serum neopterin is 9.86 nmole/l or more, autoimmune oophoritis is diagnosed.
EFFECT: using the declared technique enables improving early diagnosis of autoimmune oophoritis with normogonadotropic hypoovarianism and unspecified oligomenorrhea.
1 tbl, 3 ex
SUBSTANCE: examination is preceded by three cycles of serum freezing and thawing for 20 and 10 minutes respectively, mixture disintegration, agitation at 120 vibrations per minute for 30 minutes. That is followed by measuring apolipoprotein B, lipoprotein(a), a relation thereof, apolipoprotein A-I, total cholesterol, triacylglycerol, and a decrease of the relation of apolipoprotein B to lipoprotein(a) by 20% and more, apolipoprotein A-I by 30% and more, an increase of total cholesterol by 18% and more, triacylglycerol by 16% and more as compared to a reference shows progression of atherogenicity in ischemic heart disease.
EFFECT: method enables more effective assessment of progression of atherogenicity in ischemic heart disease.
FIELD: veterinary medicine.
SUBSTANCE: at the age of 1-3 days in the blood of calves the content of foetal haemoglobin, calcium and magnesium is determined. The ratio of calcium and magnesium is calculated. The clinically healthy state and the normal level of development of newborn calves is proved by the level of ratio of calcium/magnesium which is greater or equal to 2.9 and the content of foetal haemoglobin is greater than 60% of the total haemoglobin. The morphofunctional immaturity is proved by the level of the ratio of calcium/magnesium which is less than 2.9 and the content of foetal haemoglobin is less or equal to 60%. Informativeness of the proposed indicators is maintained up to 3 days age, but it is recommended to carry out the assessment of the level of development of calves at the age of 1 day, which enables to carry out more quickly the necessary measures aimed at correcting their physiological state and the prevention of possible diseases. The method enables to assess the muscle metabolism on the ratio of calcium and magnesium, and the usefulness of development of the mechanisms of the haemoglobin synthesis on the level of foetal haemoglobin, changes that occur in the majority of cases of disturbance of prenatal development and/or during birth.
EFFECT: increase in informativeness and the predictive value of the method.
3 tbl, 3 ex
SUBSTANCE: invention refers to medicine, namely to oncology, and can be used for assessing effectiveness of pharmacotherapy aiming at preventing acute postoperative pancreatitis in abdominal surgeries involving splenectomy. Substance of a method: on the first postoperative day, a patient's blood plasma is examined for total BAEE-esterase activity and α-1-proteinase inhibitor activity; a coefficient of 'BAEE-esterase activity/α-1-proteinase inhibitor is calculated. If the derived coefficient is less than 20 units, the applied method for preventive treatment is predicted to be effective, while the value increasing above 20 units shows ineffectiveness of preventive treatment. The presented method is implemented on the day of blood sampling - on the first postoperative day that enables taking adequate measures in a combination with the measures to prevent developing acute postoperative pancreatitis.
EFFECT: invention provides specificity of the method for prediction of preventive effectiveness of 84,1%, and for prediction of ineffectiveness of 98,7%.
SUBSTANCE: system comprises at least two samplers installed in the holes of a bearing element set on a vessel casing. Every sampler is fitted by a tubular lead-in part on the inner installation side of the bearing element and a discharge part on the upper side of the bearing element having sections for hydraulic or pneumatic valves to be connected. Lead-in parts are of different length. The bearing element is made as a removable flange with the holes in it being connecting ports. Each tubular lead-in and discharge parts are made as separate elements. The discharge part is coupled with the connecting port permanently and the tubular lead-in part is coupled with the said port by a detachable joint. Tubular lead-in part of each sampler is made as tubes of lower, middle and upper levels installed in the flange hole on the flange installation side by means of a detachable joint.
EFFECT: universality of a unit due to the possibility of its usage in vessels for fluid products of different types.
10 cl, 5 dwg
FIELD: engines and pumps.
SUBSTANCE: invention relates to supervision of vehicle operating conditions and estimation of harmful emissions. Sampling device comprises sampler connected with exhaust pipe via inlet pipe, sampling resilient chamber arranged in portable appliance and checkout hardware. Said sampling resilient detachable chamber is equipped with remote control shutoff element. Detachable appliance is equipped with the branch pipe with outlet located in one plane with sampler end. Inlet pipe has rotary mechanism for connection with the next sampling resilient chamber and valve to shut off the ICE off-gas ingress into inlet valve. Note here that every sampling resilient chamber has a contact mechanism of sampling outage at filling the entire volume of said chamber. Besides, portable appliance has inner cooling shell and cooled duct for off-gas passage from vehicle exhaust pipe. Said checkout hardware is composed of processor connected with rotary mechanism. The latter connected the next sampling chamber with the device inlet pipe and valve that shuts off ICE off-gas flow into inlet pipe.
EFFECT: off-gas sampling under whatever operating conditions, ruled out occurrence of secondary chemical reactions.
SUBSTANCE: device for preparation, storage and transportation of dry objects of liquids comprises a dry container with an adsorption element whereon a liquid aliquot containing analysed components can be applied and dried. An adsorption element is made of a moisture absorbing porous material having the adsorption capacity not less than 40 mg per cm2 and an ability for reversible desorption of dry components in an immersible sample solution of at least a working area of the adsorption element with the dry sample in the sample solution with an effectiveness of 30% at the end of no more than 300 sec after the contact of the dry sample with the sample solution.
EFFECT: invention enables obtaining the reproducible results of the analysis of biological liquids, facilitates the structure of the sorbent element.
10 cl, 4 tbl, 3 ex
SUBSTANCE: group of inventions relates to the field of technology of cyclic taking of plant samples from clamps, pits, trenches, haystacks, mows and other repositories in agriculture in determining quality indicators of feed and can also be used when taking samples of other non-free-flowing materials, such as peat, soil and snow. The method of taking plant samples is that during rotation of the device of taking the plant samples the auger retraction of the cutting crown into the feed monolith and the auger feeding of the cut-out feed in the storage unit are provided. At that the recording by the fluid content gage of the volume of the taken plant sample is provided, as well as its removal from the moving storage unit. The device of taking the plant samples comprises a shaft with a cylindrical part of the auger and a lower conical auger tip. In the area of the base of the tip the cutting crown is installed and secured with at least one pin, with mortices-hooks at the upper part, by which it docks with projections of the storage unit when turning its projections in the mortices-hooks of the crown in the opposite direction with respect to rotation in cutting the monolith of the feed. The storage unit has free rotation and movement along the shaft with the cylindrical part of the auger for releasing the crown from the hook and extracting the sample taken. In addition, the storage unit is equipped with a mechanical fluid content gage of the volume of the sample taken in the form of a plate resting on the cylindrical part of the auger, connected to the recording tube, sliding along the smooth part of the shaft with the cylindrical part of the auger.
EFFECT: monitoring the filling of the storage unit, which enables to provide the accuracy of determining the volume of the sample taken at a given depth.
2 cl, 3 dwg, 1 ex
SUBSTANCE: pathomorphological determination of the prescription of myocardial infarction is ensured by fixing a tissue sample and placing it into paraffin. Sections are prepared, de-waxed, heated, washed in a buffer solution, incubated in a moisture chamber and processed with a developing agent, dehydrated and enclosed by a medium. The reagent is presented by matrix metalloprotease 9 antibodies in dilution 1:100-1:250. The sections are incubated with the reagent at a temperature of 25°C and a relative humidity of 100% for 60 minutes. If the microscopy detects the bright-coloured neutrophils in peri-infarction vessels and within the infarction zone, the prescription is stated to be 2 hours to 1 day. If observing the neutrophil degranulation and the bright colour of an extracellular matrix within the infarction, the prescription is 1 to 2 days. If the coloured fibroblasts are found in the infarction border, the prescription is stated to be within 3 to 30 days.
EFFECT: method enables differentiating the prescription of myocardial infarction within 2 hours to 30 days.
1 ex, 3 dwg
FIELD: food industry.
SUBSTANCE: method envisages the sample acid hydrolysis, the hydrolysate filtration and chromatographic separation with subsequent automatic identification and quantitative evaluation of amino acids content using an automatic analyser. The invention allows to determine amino acids in the food product proteins composition with amino acids content equal to nearly 0.1-3.5 g/100 g of the product (1.5-17 g/100 g of protein) with application of sequential elution of amino acids with a buffer solutions mixture and simultaneous detection of the components at two wave lengths being 440 and 570 nm.
EFFECT: acceleration of the process of amino acids isolation from the food product and determination accuracy enhancement due to losses decrease and highly sensitive material application.
SUBSTANCE: submersible end of bearing pipe is equipped with metering head with submersible end and circumferential side surface. Said metering head with submersible end is furnished with at least one transducer or inlet for samples chamber arranged inside this device. Note here that said circumferential side surface of bearing pipe or metering head with accommodates inlet extending through intake channel into forechamber arranged inside said pipe or metering head. Forechamber end opposite metering head submersible end has inlet extending into slag sampling chamber arranged inside the device on forechamber side opposite said submersible end.
EFFECT: high-quality samples, precise analysis.
13 cl, 3 dwg
FIELD: machine building.
SUBSTANCE: aspirator-dust sampler consists of a casing, a diaphragm pump with electric drive, a system to stabilise the volume speed of air pumping, systems to measure the volume of the pumped air, a sampling tube and a filter holder with a filter. The diaphragm pump is made as two chambers being set towards each other, rigidly interconnected and driven by an eccentric mechanism. The eccentric mechanism is installed on the electric motor axis so that the front diaphragm position of one chamber corresponds to the opposite diaphragm position of the other chamber. Suction of air into one chamber is accompanied by the discharge of air from the other chamber. Suction valves are set on the movable diaphragms, and the discharge valves - on the stationary chambers' casing. Both chambers are the walls of the air-tight pump casing connected to a suction branch pipe in which a rarefaction sensor is built-in. The two-chamber pump is placed in another external air-tight casing where air from the chambers is discharged to and one wall of which is replaced by a rubber diaphragm serving as a damper together with the inner casing space. An air mass flow metre is built-in in the other wall. The rarefaction sensor and the flow metre are connected to a motor mode control unit and to the unit for the data on air flow, volume of pumped air, weight of dust on the filter and dust concentration.
EFFECT: improved accuracy of sampling and measuring the pumped air volume, keeping and measuring the constant volume speed of air pumping through the filter with dust residues, increased reliability of aspirator performance both at dust sampling and in the course of operation, simplified valve design, simplified measuring procedure for pumped air volume reduced to standard conditions.
3 cl, 1 dwg
FIELD: testing equipment.
SUBSTANCE: prismatic sample has a prism shape, longitudinal and transverse planes of symmetry, two side ledges, arranged longitudinally, at the ends of the prism - support surfaces, and in its central part - surface of loading with a transverse test load. The prismatic sample is additionally equipped with inclined support surfaces arranged on side longitudinal ledges of the prism and characterised by angles of inclination to the longitudinal plane of the prism symmetry 5…20°.
EFFECT: simplification and reduction of cost of prismatic sample testing process with concentrators of mechanical stresses in complex stressed condition, provision of necessary accuracy of modelling of a type of stressed-deformed condition of structure material in focus of its damage.
2 cl, 4 dwg
SUBSTANCE: invention refers to a medical sampling container, particularly to a modified multifunctional sampling container. The container comprises a body (1), a lid (2) on a body (1) opening, a fixed rotating rod (4) mounted on the lid (2) and freely rotating about the lid (2), and a sampling spoon (5) placed at the bottom of the fixed rotating rod (4). The container comprises a mesh filter (3) placed inside the body (1) perpendicular to the lid (2), and a separator connected to the bottom of the mesh filter (3). The above mesh filter (3) and the separator divide the container body (1) on a pump-down chamber (11) and a pump-off chamber (12).
EFFECT: preventing laboratory contamination and contagion, as well as providing odour-control treatment in the laboratory.
7 cl, 3 dwg
SUBSTANCE: invention relates to devices for carrying out laboratory tests. The device for carrying out chemical laboratory tests with electrical current using a micromethod has a housing, a board with flanges - supports and cells, two of which are joined by channels, holding electrode blocks and retaining devices. The housing of the device is in form of a hollow box, the front wall of which slants. There is an outer horizontal flange along the perimetre of the box. On the front flange, there is a rectangular groove, and side flanges have vertical walls which serve as supports for the housing. On the front slanted wall of the box, there is an indicator and spring-loaded fasteners with insulated buttons, the housing of which is connected to a direct current source. The cells are spherical, and the channel is V-shaped.
EFFECT: invention allows for making a simple, compact device, which allows for visual carrying out of chemical laboratory tests with electric current.
6 cl, 5 dwg