Method of determining parameters of transformable and inert organic carbon in soils

FIELD: agriculture.

SUBSTANCE: invention relates to the field of soil biology and agroecology, and can be used as criteria for evaluating the soil fertility and potential emission of carbon dioxide with soils in climate change. The method comprises determining the total content of organic carbon in the soil sample (C content), the amount of potentially mineralised organic carbon (C pm) upon incubation of the same sample, resulting in the calculation of the transformed organic carbon content (C trans). The amount of inert organic carbon (C inert) is calculated by the formula C inert = C content - C trans.

EFFECT: acceleration and simplification of determining is achieved.

1 ex, 2 tbl


The invention relates to the field of soil biology and Agroecology. It can be used as a diagnostic criterion of soil fertility, as well as estimates of potential emissions of CO2soils under global climate change.

The prototype [2, 5] is to determine the minimum content of organic carbon of the soil on a permanent clean couple in a long-term field experience (>20 years).

In accordance with Gershenson (1992) gross organic carbon (shaft) consists of inert (With inert), practically not involved in the processes of transformation, and the transformed organic carbon (TRANS). Inert fraction mainly depends on habitat conditions, and convertible, which is easily decomposed in soils, land use systems, including various agricultural practices.

The content of the inert organic carbon identically minimum, most correctly and accurately defined in perpetuating pure Paravani soil.

The contents of transforming organic carbon calculated by the formula

With TRANS = shaft With inert [2, 3].

The main disadvantage of the method definition With inert and TRANS is the need for prolonged field experience (>20 years) with permanent clean provenien soil, requiring significant time, mA is arealnykh and financial costs.

The aim of the invention is accelerated more than 350 times in comparison with the existing prototype definition With TRANS and inert in the soil without conducting long-term field experiments with permanent clean steam.

The method is as follows. In a sample of soil sample to determine the content of organic With Val (% of soil mass) by Tyurin method with spectrophotometric end. In another sample of the same sample to determine the production of S-CO2a 20-day incubation period at a temperature of 22°C and humidity 60% PPV. Next, using equations derived empirically for different taxa of soil, calculate the number of potentially mineralizing carbon, and then using special equations for these same taxa of soil aggregates With the TRANS. Values inert find the difference between values With the shaft and With the TRANS.

A reasonably accurate forecast of the availability of soil potentially mineralizers organic carbon PM without long-term incubation of samples, simulating the vegetation period, a calculation according to the cumulative production WITH CO2(when calculating the estimate of the amount of carbon dioxide is carried out in such carbon) for the 20-day incubation period, using obtained for sod-podzolic soil (equation 1), typical Chernozem (equation 2) and you alotenango Chernozem (equation 3) according to

With PM=5.54+2.04·-CO2r=0.969, n=10 (1),

With PM=13.56+1.63·-CO2r=0.999, n=6 (2),

With PM=4.95+1.94·-CO2=0.998, n=11 (3).

In turn, if the data content in the soil With gr (%) it is possible to calculate the approximate levels in the soils With TRANS (%), using special equations for sod-podzolic soil (equation 4), typical Chernozem (equation 5) and leached Chernozem (equation 6)

With TRANS=7.212·PM-0.146 r=0.951, n=6 (4),

With TRANS=5.290·PM+0.347 r=0.934, n=5 (5),

With TRANS=8.932·PM+0.056 r=0.962, n=10 (6).

An example of the calculation. As an example, consider the typical Chernozem loam Kursk region, experience 2, options permanent pure steam from 1964 and virgin steppe.

The prototype. In the soil samples these options determine the content of the organic With the shaft by Tyurin method with spectrophotometric end [1]. For soil sample permanent pure steam With Val = inert = 2,665, % of soil mass, and for such of virgin steppe With Val = 3,970, % of soil mass ≠ With inert. Then according to the formula for sample permanent pure steam With TRANS = shaft With inert = 0, and for any virgin steppe With TRANS = 3,970, % by weight of the soil - 2,665, % by weight of soil = 1,305, % of soil mass (table 1).

The proposed method. Consider the example of soil samples of the same options. As noted above, the content of experimentally identified Val = inert = 2,665% of soil mass) for sample pair of permanent and shaft=3,970 (% of soil mass) to that of virgin steppe. Is the 20-day incubation of samples according to [4]. On the basis of data on cumulative production WITH CO2when 20-day incubation of these samples are calculated first value With the PM according to equation (2), and then TRANS by equation (5). For sample version of virgin steppe calculated With TRANS = 1,333 (% of soil mass), which is close enough (statistically significant at p=0,95) experimentally determined With TRANS = 1,305 (% of soil mass). Knowing With the shaft and With the TRANS for a sample of typical Chernozem virgin steppe, can be calculated With inert for typical Chernozem Kursk region (3,970-1,333=2,637, % of soil mass), which corresponds to the experimentally determined in the sample permanent pure steam - 2,665 (% of soil mass) (table 2).

In table 1 presents the results of experimental determination of gross organic carbon in samples of sod-podzolic soils of the Vladimir region, typical Chernozem Kursk region and leached Chernozem of the Novosibirsk region in conditions of long-term field experiments. On versions with permanent provenien soils With Val = inert, and TRANS = 0. In all other cases, the transformable content of organic carbon was calculated by the formula With TRANS = shaft With inert and it was always >0.

Are given in table 2 information indicates the absence of doscover the x differences (p=0,95) between the data From the trance, received the prototype in long-term field experiment, and those with the proposed method, using the metric of potentially mineralizing carbon PM, installed in the laboratory conditions of 20-day incubation of the soil sample at a temperature of 22°C and humidity 60% PPV with quantitative account of spin-off CO2.

It is recommended that the content of the transformed organic carbon in soils with friction organic carbon content from 0.5 to 1.5% With the organization of soil mass to use equations (1) and (4); from 1.5 to 3.5% With the organization of soil mass - equation (2) and (5); from 3.5 to 5.5% With the organization of soil mass - equation (3) and (6) (table 2).


1. Dyakonov C.V. research Methods of organic substances in lysimetric waters, soil solutions and other natural objects.// Methods of stationary soil study. - M.: Nauka, 1977. S-226.

2. Kerssens M Is the content of humus to soil fertility and nitrogen cycle. Dedicated to the 100th anniversary of the birthday of Professor, academician Ivina.// Soil science. 1992. No. 10. S-131.

3. Kogut BM Principles and methods content evaluation transforming organic matter in arable soils.// Soil science. 2003. No. 3. S-316.

4. Semenov V.M., Ivannikova L.A., Kuznetsova T.V. Laboratory diagnostics of biological ka is esta soil organic matter.//In kN. Research methods of soil organic matter. 2005. VNIPTIOU. Vladimir. S-230.

5. Körschens M. Die Abbangigkeit der organishchen Bodensubstanz von Standortfactoren ind acker-und planzenbaulichen Massnahmen, ihre Beziehungen zu Bodeneigenschaften und Ertrag compounds Ableitung von erstenBodenftuchtbarkeitskennziffern für den Gehalt des Bodens an organischer Substanz. - Berlin:Akad. Landwirtsch. - Wiss. The DDR. Diss.B. 1980.

Table 1.
The content of total organic carbon (shaft) and transformed organic (TRANS) in non-arable and arable soils stationary experiments, %
№p/pOptionWith ValWith TRANS
Sod-podzolic sandy loam soil. Experience 1
1Permanent pure steam from 19680.487Not ODA.
2Accumulation of 20011.0870.600
3The rotation of 1968, without fertilizer (b/a)0.5220.035
4The same, manure 20 t/ha annually.832 0.345
5The same, manure 10 t/ha + N50P25K60 annually0.7340.247
6Same, N100P50K120 annually0.6200.133
7The same, manure 10 t/ha + N100P50K120 annually0.7630.276
The typical Chernozem loam. Experience 2
8Permanent pure steam from 19642.665Not ODA.
9Virgin steppe3.9701.305
10Permanent winter wheat since 1964, b/a3.1250.460
11Same, NPK3.4500.785
12The rotation of 1968, b/a3.3200.655
13The same, manure + NPK3.5550.890
The leached Chernozem medium. Experience 3
14Permanent pure steam since 19972.865Not ODA.
16Permanent wheat since 1997, b/aOf 3,5500.685
17The rotation of 2001, b/y, the steam cleaner without straw3.4930.628
18Same, pairs of clean straw3.6130.748
19Same, couples busy with straw3.6870.822
20Same, pairs of green manure with straw3.7170.852
21The rotation of the 2001, N40-80P40, steam cleaner without straw3.6270.762
22Same, pairs of clean straw3.7030.838
23Same, couples busy with straw3.7270.862
24Same, pairs of green manure with straw3.8530.988

Table 2.
The comparison of the contents of the convertible (With TRANS) organic substances obtained by the prototype in long-term field experiments (I) and calculated by the proposed method (II) with 20 days ' incubation of the soil, t05-criterion
№p/p∗)With trance % of soil mass
Sod-podzolic sandy loam soil, experience 1
30.035 0.103
The typical Chernozem loam, experience 2
The leached Chernozem medium, experience 3
)Rooms variants of the experiments are the same as in table 1

The method for determining indicators transformable and inert carbon in soils, including the determination of organic carbon, is great for the present, however, which soil sample is divided into two samples, one of them incubated for 20 days at a temperature of 22°C and at a humidity of 60% PPV with a quantitative accounting of evolved carbon dioxide, followed by calculation of the content of potentially mineralizing carbon and further calculation of the content, transforming carbon and the other to determine the content of total organic carbon by difference in the content of total and transformable determine the carbon content of the inert carbon.


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